Keterangan : y = nilai absorbansi x = konsentrasi asam askorbat

(1)

LAMPIRAN

Lampiran 1. KURVA STANDAR ASAM ASKORBAT

Konsentrasi asam askorbat (ppm)

8 16 20 24

32

40

Absorbansi 1

0,5017

0,3582

0,2925

0,2158

0,0861

0,0133

Absorbansi 2

0,5514

0,3682

0,2993

0,2308

0,0798

0,0182

Absorbansi 3

0,5159

0,3842

0,3185

0,2467

0,1199

0,0221

y = -0.016x + 0.6307 R2 = 0.9815

-0.1 0 0.1 0.2 0.3 0.4 0.5 0.6

0 5 10 15 20 25 30 35 40 45

konsentrasi asam askorbat

a

b

so

rb

an

si

Berdasarkan hasil pembuatan kurva standar untuk analisa asam askorbat, diperoleh

persamaan sebagai berikut:

y = -0,016x +0,6307

dengan R

2

= 0,9815

Keterangan : y = nilai absorbansi

x = konsentrasi asam askorbat

48

(2)

Konsentrasi quercetin (ppm)

10 20 30 40 50 60 70 80 90

Absorbansi

1

0,0875 0,2574 0,4530 0,4949 0,6561 0,6946 0,8031 0,9111 1,0314

Absorbansi

2

0,1501 0,2612 0,3856 0,4930 0,6531 0,7158 0,8203 0,9248 1,0640

Absorbansi

3

0,0930 0,2850 0,3861 0,4847 0,6514 0,7205 0,8206 0,9315 1,0441

x = konsentrasi quercetin

Keterangan : y = nilai absorbansi

dengan R

2

= 0,9892

y = 0,0112x + 0,041

Berdasarkan hasil pembuatan kurva standar untuk analisa total fenol, diperoleh

persamaan sebagai berikut:

Lampiran 2. KURVA STANDAR TOTAL FENOL

kurva standar total fenol

y = 0.0112x + 0.041 R2 = 0.9892

0 0.2 0.4 0.6 0.8 1 1.2

0 20

Konsent

ab

s

o

rb

an

s

i

40 60 80 100

rasi quercetin (ppm)

49

(3)

50

Lampiran 3. HASIL OBSERVASI METODE PEMASAKAN PADA 5 RUMAH MAKAN

Tgl Nama

Rumah Makan

Jns Pemasakan

Jns Sayur

Jumlah

Sayur

Jumlah

Air

Jumlah

Minyak

Proses Pemasakan

09-10-09

Kol putih

55 g

Kembang kol

80 g

Agathis,

Plamongan Indah

Perebusan

- Cap Cay kuah

Sawi hijau

50 g

250 ml

Sayur dimasukkan ke air mendidih

(±100

o

C) dan dimasak selama 4 menit

44 detik

16-10-09 Sawi

putih

100

g

Kembang kol

30 g

Steamboat

House, DP Mall

Perebusan

-

steamboat

Sawi sendok

30 g

500 ml

Sayur dimasukkan ke dalam air

mendidih (98

o

C) dan direbus selama

2-3 menit

17-10-09

Kailan

± 50 ml

Sawi

putih

Kembang

kol

Penumisan

- Ca aneka sayur

Sawi hijau

±50-60 g/

jenis sayur

±100 ml

sebelum ditumis, Kailan direbus ± 3

menit dalam ± 220 ml air mendidih.

Selanjutnya, seluruh sayuran ditumis

selama 53 detik (suhu produk: 88

o

C)

Kembang

kol

Sawi

putih

Sawi

hijau

Kol

Kelapa Gading,

Jl. Gajah Mada

Perebusan

- Cap cay kuah

Brokoli

± 40 g/

jenis sayur

±440 ml

Sayuran dimasukkan ke dalam air

mendidih (±100

o

C) dan dimasak

selama 2 menit

(suhu produk: 91

o

C)

28-11-09 Ratna

Berkah,

Gombel

Penumisan

- ca sawi sendok

Sawi sendok

220 g

±180 ml

± 25 ml

Sayuran dimasukkan ke dalam

minyak panas, lalu ditumis selama ±

1,5-2 menit

(suhu produk: 83,3

0

C)

Penumisan

- ca brokoli

Brokoli

+ sayuran lain

(jamur, wortel,

kapri)

± 130 g

hingga ±

200 g

±180 ml

± 25 ml

Sayuran dimasukkan ke dalam

minyak panas, kemudian ditumis

selama ± 1,5-2 menit (suhu produk:

82,1

0

C)

11-12-09 Mitra

Keluarga,

Bukit Sari

Penumisan

- ca sawi sendok

sawi sendok

± 160 g

±430 ml

(untuk

merebus)

±15 ml

Mula-mula sawi sendok direbus

dalam air mendidih selama 30 detik;

Kemudian baru ditumis selama 45

detik (suhu produk: 75

0

C)

(4)

Lampiran 4. HASIL UJI

HARDNESS

BEBERAPA SAYURAN

BRASSICA

Perlakuan

Rebus 2 menit

Kukus 2 menit

Kukus 3 menit

Kukus 4 menit

Hardness Sawi

sendok (gf)

2153,04 ±

202,74

a

2323,79 ±

549,96

a

2481,81 ±

251,41

a

2320,71±

361,89

a

Hardness

Brokoli (gf)

3625,23 ±

632,93

a

4712, 89 ±

845, 63

b

4270,03 ±

1349,71

ab

4798,02 ±

1070,88

b

Hardness Sawi

Hijau (gf)

1472, 41 ±

661,52

a

1266,30 ±

498,11

a

1458,23 ±

570,84

a

2454,27 ±

572,12

b

Hardness Kol

(gf)

2236,09 ±

1780,87

a

3593,30 ±

1559,35

a

2758,88 ±

1128,92

a

3570,24 ±

871,97

a

Hardness Sawi

Putih (gf)

1789,28 ±

464,95

a

2160,57 ±

325,67

b

2210,46 ±

444,56

b

2308,77 ±

163,57

b

●

Semua nilai merupakan

mean

± standar deviasi

●

Nilai dengan

superscript

yang berbeda antar kolom menunjukkan adanya perbedaan nyata

antar perlakuan (p

≤

0,05) dengan menggunakan uji wilayah Duncan

51

(5)

LAMPIRAN 5. ANALISA VARIANSI

ONE-WAY

TERHADAP

HARDNESS

BEBERAPA SAYURAN

BRASSICA

ANOVA

Sum of Squares df Mean Square F Sig. Sawi sendok Between Groups _486662.628 ₃ _162220.876 _1.207 _.323 Within Groups 4301838.816 32 134432.463

Total _4788501.444 ₃₅

brokoli Between Groups 7776844.782 3 2592281.594 2.539 .074 Within Groups _32673611.428 ₃₂ _1021050.357

Total 40450456.210 35

Sawi hijau Between Groups _7755556.428 ₃ _2585185.476 _7.723 _.001 Within Groups 10711239.567 32 334726.236

Total _18466795.996 ₃₅

Kol Between Groups 11813405.553 3 3937801.851 2.062 .125 Within Groups _61102770.738 ₃₂ _1909461.586

Total 72916176.291 35

Sawi putih Between Groups _1393259.237 ₃ _464419.746 _3.398 _.030 Within Groups 4372997.125 32 136656.160

Total 5766256.362 35

Sawi sendok

Duncan

Subset for alpha = .05 Perlakuan

N

1

rebus 2 menit _{9 2153.037504} kukus 4 menit 9 2320.709569 kukus 2 menit _{9 2323.790351} kukus 3 menit 9 2481.809724

Sig. _.090

Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 9.000.

brokoli

Duncan

N

1 2

rebus 2 menit 9 3625.224777

kukus 3 menit ₉ _4270.028139 _4270.028139 kukus 2 menit 9 4712.892181 kukus 4 menit ₉ _4798.015619

Sig. .185 .304

52

(6)

53

sawi hijau

Duncan

N

1 2

kukus 2 menit 9 1266.301864 kukus 3 menit _{9 1458.232525} rebus 2 menit 9 1472.409679

kukus 4 menit ₉ _2454.267873

Sig. .483 1.000

kol

Duncan

N

1

rebus 2 menit _{9 2236.086059} kukus 3 menit 9 2758.875863 kukus 4 menit _{9 3570.236999} kukus 2 menit 9 3593.300551

Sig. .064

sawi putih

Duncan

N

1 2

rebus 2 menit 9 1789.280786

kukus 2 menit ₉ _2160.570167 kukus 3 menit 9 2210.459620 kukus 4 menit ₉ _2308.765295

Sig. 1.000 .430

(7)

LAMPIRAN 6. ANALISA NORMALITAS DATA PENGUJIAN KOMPONEN

KIMIAWI SAWI PUTIH DAN SAWI SENDOK

Tests of Normality

Kolmogorov-Smirnov(a) Perlakuan

Statistic df Sig.

AO sawi sendok Segar .214 6 .200(*)

Rebus _.243 ₆ _.200(*)

Kukus .316 6 .062

Tumis _.199 ₆ _.200(*)

Askorbat sawi sendok Segar .303 6 .091

Rebus .225 6 .200(*)

Kukus _.156 ₆ _.200(*)

Tumis .242 6 .200(*)

Fenol sawi sendok segar .246 6 .200(*)

rebus _.289 ₆ _.129

kukus .219 6 .200(*)

tumis _.175 ₆ _.200(*)

Kadar air sawi sendok Segar _.109 ₁₈ _.200(*) Rebus .125 18 .200(*)

Kukus _.199 ₁₈ _.058

Tumis .138 18 .200(*) AO sawi putih Segar _.273 ₆ _.184

Rebus _.151 ₆ _.200(*)

Kukus .204 6 .200(*)

Tumis _.266 ₆ _.200(*)

Askorbat sawi putih Segar .187 6 .200(*)

Rebus _.284 ₆ _.142

Kukus .277 6 .169

Tumis _.224 ₆ _.200(*)

Fenol sawi putih Segar _.298 ₆ _.105

Rebus .312 6 .069

Kukus _.293 ₆ _.116

Tumis .299 6 .101

Kadar air sawi putih Segar .197 18 .063 Rebus _.144 ₁₈ _.200(*)

Kukus .196 18 .067

Tumis _.121 ₁₈ _.200(*) * This is a lower bound of the true significance.

a Lilliefors Significance Correction

54

(8)

LAMPIRAN 7. ANALISA VARIANSI

ONE-WAY

DATA PENGUJIAN

KOMPONEN KIMIAWI PADA SAWI SENDOK

ANOVA

Sum of

Squares df

Mean

Square F Sig. AO sawi sendok Between Groups _2417.875 ₃ _805.958 _54.941 _.000 Within Groups 293.392 20 14.670

Total _2711.267 ₂₃

Askorbat sawi sendok Between Groups 60.880 3 20.293 12.270 .000

Within Groups _33.078 ₂₀ _1.654

Total 93.958 23

Fenol sawi sendok Between Groups 1302380.201 3 434126.734 46.874 .000 Within Groups 185232.883 20 9261.644

Total 1487613.084 23

kdr_air_sawi_sendok Between Groups _1501.145 ₃ _500.382 _901.196 _.000

Within Groups 37.756 68 .555

Total _1538.902 ₇₁

Post Hoc Tests

Homogeneous Subsets

AO sawi sendok

Duncan

Subset for alpha = .05

Perlakuan N 1 2 3

segar 6 31.6219

rebus ₆ _38.9802

tumis 6 52.4310

kukus ₆ _56.5152

Sig. 1.000 1.000 .080 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 6.000.

askorbat sawi sendok

Duncan

Subset for alpha = .05 Perlakuan N 1 2 segar 6 26.1642

tumis ₆ _29.4733

rebus 6 29.9342

kukus ₆ _30.0242

Sig. 1.000 .492

fenol sawi sendok

55

(9)

56

Duncan

Perlakuan N 1 2 3

rebus 6 881.6042

tumis ₆ _1252.7232

kukus 6 1312.5030

segar ₆ _1528.5417

Sig. 1.000 .295 1.000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 6.000.

kdr air sawi sendok

Duncan

perlakuan N 1 2 3

tumis 18 83.9365

segar 18 94.2049 kukus ₁₈ _94.3496

rebus 18 94.8469

Sig. _1.000 _.562 _1.000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 18.000.

(10)

LAMPIRAN 8. ANALISA VARIANSI

ONE-WAY

DATA PENGUJIAN

KOMPONEN KIMIAWI PADA SAWI PUTIH

ANOVA

Sum of

Squares df

Mean

Square F Sig. AO sawi putih Between Groups 96.561 3 32.187 6.213 .004

Within Groups _103.613 ₂₀ _5.181

Total 200.174 23

Askorbat sawi putih Between Groups _205.292 ₃ _68.431 _11.129 _.000

Within Groups 122.982 20 6.149

Total _328.273 ₂₃

Fenol sawi putih Between Groups 66168.042 3 22056.014 2.225 .117 Within Groups _198229.150 ₂₀ _9911.458

Total 264397.192 23

Kdr air sawi putih Between Groups _1496.528 ₃ _498.843 _2278.149 _.000

Within Groups 14.890 68 .219

Total _1511.418 ₇₁

Post Hoc Tests

Homogeneous Subsets

AO sawi putih

Duncan

Subset for alpha = .05 Perlakuan N ₁ ₂ rebus ₆ _17.6949

segar 6 18.1468

kukus ₆ _21.5112

tumis 6 22.2583

Sig. _.735 _.576

askorbat sawi putih

Duncan

Perlakuan N 1 2 3

segar 6 16.2217

rebus ₆ _18.6192 _18.6192

tumis 6 21.4725 21.4725

kukus ₆ _23.9858

Sig. .110 .060 .094

57

(11)

58

fenol sawi putih

Duncan

Subset for alpha = .05 Perlakuan N 1 2 rebus ₆ _262.3631

tumis 6 330.1309 330.1309 segar ₆ _372.9524 _372.9524

kukus 6 401.8929

Sig. _.082 _.251

kdr air sawi putih

Duncan

perlakuan N ₁ ₂ ₃

tumis ₁₈ _86.0464

kukus 18 96.3846 segar ₁₈ _96.4063

rebus 18 96.9020

Sig. _1.000 _.890 _1.000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 18.000.

(12)

LAMPIRAN 9. ANALISA KORELASI AKTIVITAS ANTIOKSIDAN, ASAM

ASKORBAT, DAN TOTAL FENOL PADA SAWI SENDOK

►

Sawi sendok Segar

Correlations

AO sendok segar

Askorbat sendok segar

Fenol sendok segar AO sendok segar Pearson Correlation ₁ _.532 _-.636

Sig. (2-tailed) .278 .174

N ₆ ₆ ₆

Askorbat sendok segar Pearson Correlation .532 1 -.919(**)

Sig. (2-tailed) _.278 _.009

N 6 6 6

Fenol sendok segar Pearson Correlation -.636 -.919(**) 1

Sig. (2-tailed) .174 .009

N 6 6 6

** Correlation is significant at the 0.01 level (2-tailed).

►

Sawi sendok rebus

Correlations

AO sendok rebus

Askorbat sendok rebus

Fenol sendok rebus AO sendok rebus Pearson Correlation ₁ _-.204 _-.345

Sig. (2-tailed) .698 .503

N ₆ ₆ ₆

Askorbat sendok rebus Pearson Correlation -.204 1 .844(*)

Sig. (2-tailed) _.698 _.034

N 6 6 6

Fenol sendok rebus Pearson Correlation _-.345 _.844(*) ₁

Sig. (2-tailed) .503 .034

N 6 6 6

* Correlation is significant at the 0.05 level (2-tailed).

►

Sawi sendok kukus

Correlations

AO sendok kukus

Askorbat sendok kukus

Fenol sendok kukus AO sendok kukus Pearson Correlation ₁ _.113 _.175

Sig. (2-tailed) .831 .740

N ₆ ₆ ₆

Askorbat sendok kukus Pearson Correlation .113 1 -.814(*)

Sig. (2-tailed) _.831 _.049

N 6 6 6

Fenol sendok kukus Pearson Correlation _.175 _-.814(*) ₁

Sig. (2-tailed) .740 .049

N 6 6 6

* Correlation is significant at the 0.05 level (2-tailed).

59

(13)

60

►

Sawi sendok tumis

Correlations

AO sendok tumis

Askorbat sendok tumis

Fenol sendok tumis AO sendok tumis Pearson Correlation 1 -.132 -.046

Sig. (2-tailed) _.804 _.931

N 6 6 6

Askorbat sendok tumis Pearson Correlation _-.132 ₁ _-.443

Sig. (2-tailed) .804 .379

N ₆ ₆ ₆

Fenol sendok tumis Pearson Correlation -.046 -.443 1

Sig. (2-tailed) _.931 _.379

N 6 6 6

(14)

LAMPIRAN 10. ANALISA KORELASI AKTIVITAS ANTIOKSIDAN, ASAM

ASKORBAT, DAN TOTAL FENOL PADA SAWI PUTIH

►

Sawi putih Segar

Correlations

AO putih segar

Askorbat putih segar

Fenol putih segar AO putih segar Pearson Correlation ₁ _-.335 _.101

Sig. (2-tailed) .516 .849

N ₆ ₆ ₆

Askorbat putih segar Pearson Correlation -.335 1 -.494

Sig. (2-tailed) _.516 _.319

N 6 6 6

Fenol putih segar Pearson Correlation .101 -.494 1

Sig. (2-tailed) .849 .319

N 6 6 6

►

Sawi putih rebus

Correlations

AO putih rebus

Askorbat putih rebus

Fenol putih rebus AO putih rebus Pearson Correlation 1 -.653 .590

Sig. (2-tailed) _.160 _.218

N 6 6 6

Askorbat putih rebus Pearson Correlation _-.653 ₁ _-.987(**)

Sig. (2-tailed) .160 .000

N ₆ ₆ ₆

Fenol putih rebus Pearson Correlation .590 -.987(**) 1

Sig. (2-tailed) _.218 _.000

N 6 6 6

** Correlation is significant at the 0.01 level (2-tailed).

►

Sawi putih kukus

Correlations

AO putih kukus

Askorbat putih kukus

Fenol putih kukus AO putih kukus Pearson Correlation ₁ _-.853(*) _.883(*)

Sig. (2-tailed) .031 .020

N ₆ ₆ ₆

Askorbat putih kukus Pearson Correlation -.853(*) 1 -.986(**)

Sig. (2-tailed) _.031 _.000

N 6 6 6

Fenol putih kukus Pearson Correlation _.883(*) _-.986(**) ₁

Sig. (2-tailed) .020 .000

N 6 6 6

* Correlation is significant at the 0.05 level (2-tailed). ** Correlation is significant at the 0.01 level (2-tailed).

61

(15)

62

►

Sawi putih tumis

Correlations

AO putih tumis

Askorbat putih tumis

Fenol putih tumis AO putih tumis Pearson Correlation 1 -.956(**) .959(**)

Sig. (2-tailed) .003 .003

N ₆ ₆ ₆

Askorbat putih tumis Pearson Correlation -.956(**) 1 -.872(*)

Sig. (2-tailed) _.003 _.023

N 6 6 6

Fenol putih tumis Pearson Correlation _.959(**) _-.872(*) ₁

Sig. (2-tailed) .003 .023

N ₆ ₆ ₆

** Correlation is significant at the 0.01 level (2-tailed). * Correlation is significant at the 0.05 level (2-tailed).

(16)

LAMPIRAN 11. ANALISA

INDEPENDENT-SAMPLE T TEST

PADA SAWI

PUTIH DAN SAWI SENDOK

Independent Samples Test

t-test for Equality of Means t df

Sig. (2-tailed)

Mean Difference

Std. Error Difference

95% Confidence Interval of the Difference

Lower Upper

AO sgr Eq var ass 6.119 10 .000 13.47333 2.20188 8.56723 18.37944 Eq var not ass 6.119 9.578 .000 13.47333 2.20188 8.53774 18.40893 AO rbs Eq var ass 18.597 10 .000 21.28500 1.14453 18.73482 23.83518 Eq var not ass 18.597 6.864 .000 21.28500 1.14453 18.56771 24.00229 AO kks Eq var ass 21.132 10 .000 35.00333 1.65639 31.31266 38.69401 Eq var not ass 21.132 9.265 .000 35.00333 1.65639 31.27259 38.73408 AO tms Eq var ass 14.507 10 .000 30.17333 2.07987 25.53908 34.80758 Eq var not ass 14.507 5.842 .000 30.17333 2.07987 25.05044 35.29623 Ask sgr Eq var ass 9.170 10 .000 9.94333 1.08430 7.52737 12.35930 Eq var not ass 9.170 5.876 .000 9.94333 1.08430 7.27656 12.61011 Ask rbs Eq var ass 16.618 10 .000 11.31333 .68080 9.79643 12.83024 Eq var not ass 16.618 5.143 .000 11.31333 .68080 9.57782 13.04885 Ask kks Eq var ass 4.318 10 .002 6.03667 1.39804 2.92164 9.15169 Eq var not ass 4.318 5.045 .007 6.03667 1.39804 2.45254 9.62079 Ask tms Eq var ass 6.312 10 .000 7.99833 1.26715 5.17495 10.82171 Eq var not ass 6.312 5.038 .001 7.99833 1.26715 4.74845 11.24822 Fnol sgr Eq var ass 14.171 10 .000 1155.58928 81.54402 973.89789 1337.28068 Eq var not ass 14.171 8.322 .000 1155.58928 81.54402 968.80773 1342.37083 Fnol rbs Eq var ass 18.670 10 .000 619.24105 33.16853 545.33696 693.14514 Eq var not ass 18.670 8.692 .000 619.24105 33.16853 543.80174 694.68036 Fnol kks Eq var ass 15.796 10 .000 910.61012 57.64965 782.15870 1039.06153 Eq var not ass 15.796 8.373 .000 910.61012 57.64965 778.69345 1042.52678 Fnol tms Eq var ass 22.317 10 .000 922.59228 41.34015 830.48069 1014.70388 Eq var not ass 22.317 5.560 .000 922.59228 41.34015 819.46475 1025.71982 Air sgr Eq var ass -10.508 34 .000 -2.20142 .20950 -2.62718 -1.77566 Eq var not ass -10.508 22.031 .000 -2.20142 .20950 -2.63587 -1.76698 Air rbs Eq var ass -15.711 34 .000 -2.05504 .13081 -2.32087 -1.78922 Eq var not ass -15.711 23.755 .000 -2.05504 .13081 -2.32516 -1.78493 Air kks Eq var ass -21.328 34 .000 -2.03618 .09547 -2.23019 -1.84216 Eq var not ass -21.328 33.829 .000 -2.03618 .09547 -2.23023 -1.84213 Air tms Eq var ass -6.610 34 .000 -2.10991 .31921 -2.75863 -1.46118 Eq var not ass -6.610 31.200 .000 -2.10991 .31921 -2.76078 -1.45903

63

(17)

LAMPIRAN 12. PROSES PEMASAKAN SAWI PUTIH DAN SAWI SENDOK

PEREBUSAN

Sawi

sendok

Sawi

putih

PENGUKUSAN

Sawi

sendok

Sawi

putih

PENUMISAN

Sawi

sendok

Sawi

putih

64