28
7. LAMPIRAN
Lampiran 1. Kurva Standar Tokoferol
Tokoferol
ppm
abs
0
0.0392
40
0.1407
80
0.2509
120
0.3866
160
0.5098
200
0.6722
y = 0.0031x + 0.0184
R² = 0.9941
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0
50
100
150
200
250
Series1
Linear (Series1)
Lampiran 2. Kurva Standar Total Fenol
y = 0.0042x + 0.0369
R² = 0.9978
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
0
50
100
150
200
250
Series1
Linear (Series1)
Total Fenol
ppm
abs
0
0.0414
50
0.2564
100
0.4265
150
0.6591
200
0.8792
Lampiran 3. Pembuatan Media MRS
broth
Medium MRS
broth
dibuat dengan mengencerkan 52,2 gram bubuk
Merck
MRS
broth
dalam 1 liter aquades, kemudian medium diaduk hingga terlarut dengan merata. Setelah
itu medium disterilkan menggunakan
autoclave
pada suhu 121
o
C selama 15 menit.
Lampiran 4. Data Absorbansi Tokoferol
Batch 1
Lampiran 5. Data Absorbansi Total Fenol
Batch 1
0 1 0.2011 0.036 0.004 25 10 10318.75 1031.875 2 0.2036 0.036 0.004 25 10 10475 1047.5 3 0.2006 0.036 0.004 25 10 10287.5 1028.75 4 0.2002 0.036 0.004 25 10 10262.5 1026.25 5 0.2037 0.036 0.004 25 10 10481.25 1048.125 6 0.2021 0.036 0.004 25 10 10381.25 1038.125 1 1 0.2051 0.036 0.004 25 10 10568.75 1056.875 2 0.2046 0.036 0.004 25 10 10537.5 1053.75 3 0.2076 0.036 0.004 25 10 10725 1072.5 4 0.2047 0.036 0.004 25 10 10543.75 1054.375 5 0.2034 0.036 0.004 25 10 10462.5 1046.25 6 0.2037 0.036 0.004 25 10 10481.25 1048.125 2 1 0.2132 0.036 0.004 25 10 11075 1107.5 2 0.2164 0.036 0.004 25 10 11275 1127.5 3 0.2148 0.036 0.004 25 10 11175 1117.5 4 0.2162 0.036 0.004 25 10 11262.5 1126.25 5 0.2157 0.036 0.004 25 10 11231.25 1123.125 6 0.2143 0.036 0.004 25 10 11143.75 1114.375 3 1 0.2214 0.036 0.004 25 10 11587.5 1158.75 2 0.221 0.036 0.004 25 10 11562.5 1156.25 3 0.2201 0.036 0.004 25 10 11506.25 1150.625 4 0.2206 0.036 0.004 25 10 11537.5 1153.75 5 0.2207 0.036 0.004 25 10 11543.75 1154.375 6 0.2218 0.036 0.004 25 10 11612.5 1161.25 4 1 0.2409 0.036 0.004 25 10 12806.25 1280.625 2 0.241 0.036 0.004 25 10 12812.5 1281.25 3 0.2401 0.036 0.004 25 10 12756.25 1275.625 4 0.2416 0.036 0.004 25 10 12850 1285 5 0.2389 0.036 0.004 25 10 12681.25 1268.125 6 0.2401 0.036 0.004 25 10 12756.25 1275.625 5 1 0.2504 0.036 0.004 25 10 13400 1340 2 0.2512 0.036 0.004 25 10 13450 1345 3 0.2503 0.036 0.004 25 10 13393.75 1339.375 4 0.2514 0.036 0.004 25 10 13462.5 1346.25 5 0.2525 0.036 0.004 25 10 13531.25 1353.125 6 0.2518 0.036 0.004 25 10 13487.5 1348.75 5.23907594 5.9116763 3.78456294 7.7055175 7.97% 11.48% 23.24% 29.77% 1277.70833 1345.41667 9.32696025 9.43246278 1036.77083 1055.3125 1119.375 1155.83333 0.00% 1.79% Stdev Ekstrak si Pengen ceran Total Fenol (ppm) Rata2 T.Fenol Total Fenol (mg/100g) % Peningkatan Hari ke-Fermentasi Spontan a b UlFermenta
si BAL
a
b
Ekstra
ksi
Pengencera
n
Total Fenol
(ppm)
Total Fenol
(mg/100g)
Rata2
T.Fenol
(mg/100g)
stdev
%
Peningkatan
0
1
0.2029
0.036
0.004
25
10
10431.25
1043.125
2
0.2031
0.036
0.004
25
10
10443.75
1044.375
3
0.2046
0.036
0.004
25
10
10537.5
1053.75
4
0.2029
0.036
0.004
25
10
10431.25
1043.125
5
0.2022
0.036
0.004
25
10
10387.5
1038.75
6
0.2033
0.036
0.004
25
10
10456.25
1045.625
1
1
0.2132
0.036
0.004
25
10
11075
1107.5
2
0.2122
0.036
0.004
25
10
11012.5
1101.25
3
0.2124
0.036
0.004
25
10
11025
1102.5
4
0.214
0.036
0.004
25
10
11125
1112.5
5
0.2108
0.036
0.004
25
10
10925
1092.5
6
0.2105
0.036
0.004
25
10
10906.25
1090.625
2
1
0.2295
0.036
0.004
25
10
12093.75
1209.375
2
0.2287
0.036
0.004
25
10
12043.75
1204.375
3
0.2268
0.036
0.004
25
10
11925
1192.5
4
0.2307
0.036
0.004
25
10
12168.75
1216.875
5
0.2304
0.036
0.004
25
10
12150
1215
6
0.231
0.036
0.004
25
10
12187.5
1218.75
3
1
0.2327
0.036
0.004
25
10
12293.75
1229.375
2
0.2325
0.036
0.004
25
10
12281.25
1228.125
3
0.2338
0.036
0.004
25
10
12362.5
1236.25
4
0.2333
0.036
0.004
25
10
12331.25
1233.125
5
0.232
0.036
0.004
25
10
12250
1225
6
0.2356
0.036
0.004
25
10
12475
1247.5
4
1
0.256
0.036
0.004
25
10
13750
1375
2
0.2564
0.036
0.004
25
10
13775
1377.5
3
0.2531
0.036
0.004
25
10
13568.75
1356.875
4
0.2573
0.036
0.004
25
10
13831.25
1383.125
5
0.2564
0.036
0.004
25
10
13775
1377.5
6
0.257
0.036
0.004
25
10
13812.5
1381.25
31.63%
1044.79167 4.9634
8.4448
1101.14583
1209.47917 9.8537
8.0177
1233.22917
18.04%
0.00%
5.39%
15.76%
9.4428
1375.20833
Ul
Hari
Lampiran 6. Analisis Data Tokoferol Fermentasi Spontan
Cas e Proces s ing Sum m ary
4 100.0% 0 .0% 4 100.0% 4 100.0% 0 .0% 4 100.0% 4 100.0% 0 .0% 4 100.0% 4 100.0% 0 .0% 4 100.0% 4 100.0% 0 .0% 4 100.0% 4 100.0% 0 .0% 4 100.0% Lama_Fermentasi Hari ke-0 Hari ke-1 Hari ke-2 Hari ke-3 Hari ke-4 Hari ke-5 Fermentas i_Spontan
N Percent N Percent N Percent
V alid Mis sing Total
Cases
Tes ts of Nor m ality
.243 4 . .932 4 .607 .239 4 . .959 4 .772 .261 4 . .881 4 .343 .251 4 . .913 4 .500 .177 4 . .989 4 .954 .214 4 . .982 4 .916 Lama_Fermentasi Hari ke-0 Hari ke-1 Hari ke-2 Hari ke-3 Hari ke-4 Hari ke-5 Fermentas i_Spontan
Statistic df Sig. Statistic df Sig. Kolmogorov-Smirnova Shapiro-Wilk
Lilliefors Signif ic ance Correc tion a.
ANOV A
Fermentas i_Spontan
10591.009
5
2118.202
228.594
.000
166.792
18
9.266
10757.801
23
Betw een Groups
Within Groups
Total
Sum of
Squares
df
Mean Square
F
Sig.
Fe rm entas i_Spontan
Dunc an
a4
234.6875
4
235.2917
4
250.2292
4
269.2708
4
275.1875
4
291.4167
.782
1.000
1.000
1.000
1.000
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Hari ke-5
Sig.
N
1
2
3
4
5
Subs et f or alpha = .05
Means f or groups in homogeneous subsets are display ed.
Uses Harmonic Mean Sample Siz e = 4.000.
a.
Tes t of Hom ogene ity of V ariance s
Fermentas i_Spontan
.824
5
18
.549
Levene
Lampiran 7. Analisis Data Tokoferol Fermentasi Penambahan BAL
Cas e Proce ss ing Sum m ary
4 100.0% 0 .0% 4 100.0% 4 100.0% 0 .0% 4 100.0% 4 100.0% 0 .0% 4 100.0% 4 100.0% 0 .0% 4 100.0% 4 100.0% 0 .0% 4 100.0% Lama_Fermentasi Hari ke-0 Hari ke-1 Hari ke-2 Hari ke-3 Hari ke-4 Fermentas i_BA L
N Percent N Percent N Percent
V alid Mis sing Total
Cases
Tes ts of Norm ality
.214 4 . .963 4 .798 .191 4 . .973 4 .861 .239 4 . .938 4 .641 .378 4 . .804 4 .110 .238 4 . .940 4 .656 Lama_Fermentasi Hari ke-0 Hari ke-1 Hari ke-2 Hari ke-3 Hari ke-4 Fermentas i_BA L
Statistic df Sig. Statistic df Sig. Kolmogorov-Smirnova Shapiro-Wilk
Lillief ors Signif icance Correc tion a.
ANOV A
Fermentas i_BA L
9233.053
4
2308.263
246.111
.000
140.684
15
9.379
9373.738
19
Betw een Groups
Within Groups
Total
Sum of
Squares
df
Mean Square
F
Sig.
Tes t of Hom ogene ity of V ariance s
Fermentas i_BA L
5.182
4
15
.008
Levene
Statistic
df 1
df 2
Sig.
Fe rm entas i_BAL
Dunc an
a4
237.0208
4
252.8750
4
265.6458
4
281.3333
4
298.6667
1.000
1.000
1.000
1.000
1.000
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Sig.
N
1
2
3
4
5
Subs et f or alpha = .05
Means f or groups in homogeneous subsets are display ed.
Uses Harmonic Mean Sample Siz e = 4.000.
ANOV A
Fermentas i_Spontan
478058.8
5
95611.761
1514.264
.000
1894.222
30
63.141
479953.0
35
Betw een Groups
Within Groups
Total
Sum of
Squares
df
Mean Square
F
Sig.
Lampiran 8. Analisis Data Total Fenol Fermentasi spontan
Cas e Proces s ing Sum m ary
6 100.0% 0 .0% 6 100.0% 6 100.0% 0 .0% 6 100.0% 6 100.0% 0 .0% 6 100.0% 6 100.0% 0 .0% 6 100.0% 6 100.0% 0 .0% 6 100.0% 6 100.0% 0 .0% 6 100.0% Lama_Fermentasi Hari ke-0 Hari ke-1 Hari ke-2 Hari ke-3 Hari ke-4 Hari ke-5 Fermentas i_Spontan
N Percent N Percent N Percent
V alid Mis sing Total
Cases
Tes ts of Nor m ality
.271 6 .191 .855 6 .172 .307 6 .079 .858 6 .181 .142 6 .200* .991 6 .991 .170 6 .200* .957 6 .795 .274 6 .178 .914 6 .464 .208 6 .200* .947 6 .719 Lama_Fermentasi Hari ke-0 Hari ke-1 Hari ke-2 Hari ke-3 Hari ke-4 Hari ke-5 Fermentas i_Spontan
Statistic df Sig. Statistic df Sig. Kolmogorov-Smirnova Shapiro-Wilk
This is a low er bound of the true signif icanc e. *.
Lilliefors Signif ic ance Correc tion a.
Tes t of Hom ogene ity of V ariance s
Fermentas i_Spontan
2.864
5
30
.031
Levene
Statistic
df 1
df 2
Sig.
Fe rm entas i_Spontan
Dunc an
a6 1038.6979
6
1053.0729
6
1118.1771
6
1145.2604
6
1275.0000
6
1356.0938
1.000
1.000
1.000
1.000
1.000
1.000
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Hari ke-5
Sig.
N
1
2
3
4
5
6
Subs et for alpha = .05
Means f or groups in homogeneous subsets are display ed.
Uses Harmonic Mean Sample Siz e = 6.000.
Lampiran 9. Analisis Data Total Fenol fermentasi Penambahan BAL
Cas e Proce ss ing Sum m ary
6 100.0% 0 .0% 6 100.0% 6 100.0% 0 .0% 6 100.0% 6 100.0% 0 .0% 6 100.0% 6 100.0% 0 .0% 6 100.0% 6 100.0% 0 .0% 6 100.0% Lama_Fermentasi Hari ke-0 Hari ke-1 Hari ke-2 Hari ke-3 Hari ke-4 Fermentas i_BA L
N Percent N Percent N Percent
V alid Mis sing Total
Cases
Tes ts of Norm ality
.260 6 .200* .828 6 .103 .145 6 .200* .995 6 .997 .154 6 .200* .967 6 .869 .215 6 .200* .948 6 .723 .200 6 .200* .941 6 .668 Lama_Fermentasi Hari ke-0 Hari ke-1 Hari ke-2 Hari ke-3 Hari ke-4 Fermentas i_BA L
Statistic df Sig. Statistic df Sig. Kolmogorov-Smirnova Shapiro-Wilk
This is a low er bound of the true signif ic anc e. *.
Lillief ors Signif icance Correc tion
a.
Tes t of Hom ogene ity of V ariance s
Fermentas i_BA L
2.068
4
25
.115
Levene
Statistic
df 1
df 2
Sig.
ANOV A
Fermentas i_BA L
387764.1
4
96941.016
2676.494
.000
905.485
25
36.219
388669.5
29
Betw een Groups
Within Groups
Total
Sum of
Squares
df
Mean Square
F
Sig.
Fe rm entas i_BAL
Dunc an
a6 1046.0417
6
1116.3542
6
1205.5729
6
1262.0833
6
1373.3333
1.000
1.000
1.000
1.000
1.000
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Sig.
N
1
2
3
4
5
Subs et f or alpha = .05
Means f or groups in homogeneous subsets are display ed.
Uses Harmonic Mean Sample Siz e = 6.000.
Lampiran 10. Analisis Data Tokoferol Tiap Perlakuan Fermentasi
Uji T Tokoferol Biji Kakao Pada 0 Jam Fermentasi
Uji T Tokoferol Biji Kakao Pada 24 Jam Fermentasi
Uji T Tokoferol Biji Kakao Pada 48 Jam Fermentasi
Group Statis tics
4
234.6875
3.29448
1.64724
4
237.0208
.18478
.09239
PERLA KUA N
Spontan 0 jam
BAL 0 jam
TOKOFEROL
N
Mean
Std. Deviation
Std. Error
Mean
Group Statis tics
4
235.2917
4.35066
2.17533
4
252.8750
1.92149
.96075
PERLAKUAN
Spontan 24 jam
BAL 24 jam
TOKOFEROL
N
Mean
Std. Deviation
Std. Error
Mean
Group Statis tics
4
250.2292
2.08653
1.04326
4
265.6458
4.51662
2.25831
PERLAKUAN
Spontan 48 jam
BAL 48 jam
TOKOFEROL
N
Mean
Std. Dev iation
Std. Error
Mean
Inde pe nde nt Sam ples Te st
13.303 .011 -1.414 6 .207 -2.33333 1.64983 -6.37032 1.70365 -1.414 3.019 .252 -2.33333 1.64983 -7.56530 2.89863 Equal variances as sumed Equal variances not assumed TOKOFEROL F Sig. Levene's Test f or Equality of Variances t df Sig. (2-tailed) Mean Dif f erence Std. Error
Dif f erence Low er Upper 95% Conf idence
Interval of the Dif f erence t-test f or Equality of Means
Inde pe nde nt Sam ples Te st
1.142 .326 -7.394 6 .000 -17.58333 2.37804 -23.40220 -11.76447 -7.394 4.127 .002 -17.58333 2.37804 -24.10620 -11.06046 Equal variances as sumed Equal variances not assumed TOKOFEROL F Sig. Levene's Test f or Equality of Variances t df Sig. (2-tailed) Mean Dif f erence Std. Error
Dif f erence Low er Upper 95% Conf idence
Interval of the Dif f erence t-test f or Equality of Means
Inde pe nde nt Sam ples Te st .120 .741 -9.269 6 .000 -23.47917 2.53297 -29.67712 -17.28121 -9.269 5.990 .000 -23.47917 2.53297 -29.67955 -17.27879 Equal variances as sumed Equal variances not assumed TOKOFEROL F Sig. Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Low er Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Uji T Tokoferol Biji Kakao Pada 72 Jam Fermentasi
Uji T Tokoferol Biji Kakao Pada 96 Jam Fermentasi
Group Statis tics
4
269.2708
1.25900
.62950
4
281.3333
3.06941
1.53471
PERLAKUAN
Spontan 72 jam
BAL 72 jam
TOKOFEROL
N
Mean
Std. Dev iation
Std. Error
Mean
Inde pe nde nt Sam ples Te st
6.017 .050 -6.197 6 .001 -15.41667 2.48764 -21.50371 -9.32962 -6.197 4.225 .003 -15.41667 2.48764 -22.18098 -8.65235 Equal variances as sumed Equal variances not assumed TOKOFEROL F Sig. Levene's Test f or Equality of Variances t df Sig. (2-tailed) Mean Dif f erence Std. Error
Dif f erence Low er Upper 95% Conf idence
Interval of the Dif f erence t-test f or Equality of Means
Inde pe nde nt Sam ples Te st
2.169 .191 -7.272 6 .000 -12.06250 1.65879 -16.12142 -8.00358 -7.272 3.982 .002 -12.06250 1.65879 -16.67642 -7.44858 Equal variances as sumed Equal variances not assumed TOKOFEROL F Sig. Levene's Test f or Equality of V ariances t df Sig. (2-tailed) Mean Dif f erence Std. Error
Dif f erence Low er Upper 95% Conf idence
Interval of the Dif f erence t-test f or Equality of Means
Group Statis tics
4
275.1875
3.50949
1.75475
4
298.6667
3.65339
1.82669
PERLAKUAN
Spontan 96 jam
BAL 96 jam
TOKOFEROL
N
Mean
Std. Dev iation
Std. Error
Mean
Lampiran 11. Analisis Data Total Fenol Tiap Perlakuan Fermentasi
Uji T Total Fenol Biji Kakao Pada 0 Jam Fermentasi
Uji T Total Fenol Biji Kakao Pada 24 Jam Fermentasi
Uji T Total Fenol Biji Kakao Pada 48 Jam Fermentasi
Group Statis tics
6 1038.6979
10.59078
4.32367
6 1046.0417
2.20676
.90091
PERLAKUAN
Spontan 0 jam
BAL 0 jam
t.f enol
N
Mean
Std. Deviation
Std. Error
Mean
Group Statis tics
6 1053.0729
4.18991
1.71052
6 1116.3542
7.56138
3.08692
PERLAKUAN
Spontan 24 jam
BAL 24 jam
t.f enol
N
Mean
Std. Deviation
Std. Error
Mean
Group Statis tics
6 1118.1771
12.77726
5.21630
6 1205.5729
8.22871
3.35935
PERLA KUA N
Spontan 48 jam
BAL 48 jam
t.f enol
N
Mean
Std. Deviation
Std. Error
Mean
Inde pe nde nt Sam ples Te st
9.135 .013 -1.663 10 .127 -7.34375 4.41653 -17.18440 2.49690 -1.663 5.433 .153 -7.34375 4.41653 -18.42979 3.74229 Equal variances as sumed Equal variances not assumed t.f enol F Sig. Levene's Test f or Equality of V ariances t df Sig. (2-tailed) Mean Dif f erence Std. Error
Dif f erence Low er Upper 95% Conf idence
Interval of the Dif f erence t-test f or Equality of Means
Inde pe nde nt Sam ples Te st
1.063 .327 -17.931 10 .000 -63.28125 3.52916 -71.14471 -55.41779 -17.931 7.806 .000 -63.28125 3.52916 -71.45486 -55.10764 Equal variances as sumed Equal variances not assumed t.f enol F Sig. Levene's Test f or Equality of Variances t df Sig. (2-tailed) Mean Dif f erence Std. Error
Dif f erence Low er Upper 95% Conf idence
Interval of the Dif f erence t-test f or Equality of Means
Inde pe nde nt Sam ples Te st 4.033 .072 -31.801 10 .000 -98.33333 3.09219 -105.223 -91.44351 -31.801 7.053 .000 -98.33333 3.09219 -105.634 -91.03261 Equal variances as sumed Equal variances not assumed t.f enol F Sig. Levene's Test f or Equality of V ariances t df Sig. (2-tailed) Mean Dif f erence Std. Error
Dif f erence Low er Upper 95% Conf idence
Interval of the Dif f erence t-test f or Equality of Means
Uji T Total Fenol Biji Kakao Pada 72 Jam Fermentasi
Uji T Total Fenol Biji Kakao Pada 96 Jam Fermentasi
Group Statis tics
6 1145.2604
4.51740
1.84422
6 1262.0833
6.41856
2.62037
PERLA KUA N
Spontan 72 jam
BAL 72 jam
t.f enol
N
Mean
Std. Deviation
Std. Error
Mean
Group Statis tics
6 1275.0000
6.87216
2.80555
6 1373.3333
3.18484
1.30021
PERLA KUA N
Spontan 96 jam
BAL 96 jam
t.f enol
N
Mean
Std. Deviation
Std. Error
Mean
Inde pe nde nt Sam ples Te st
1.244 .291 -14.086 10 .000 -87.39583 6.20443 -101.220 -73.57149 -14.086 8.539 .000 -87.39583 6.20443 -101.548 -73.24401 Equal variances as sumed Equal variances not assumed t.f enol F Sig. Levene's Test f or Equality of V ariances t df Sig. (2-tailed) Mean Dif f erence Std. Error
Dif f erence Low er Upper 95% Conf idence
Interval of the Dif f erence t-test f or Equality of Means
Inde pe nde nt Sam ples Te st
1.829 .206 -36.458 10 .000 -116.82292 3.20429 -123.963 -109.683 -36.458 8.977 .000 -116.82292 3.20429 -124.074 -109.572 Equal variances as sumed Equal variances not assumed t.f enol F Sig. Levene's Test f or Equality of V ariances t df Sig. (2-tailed) Mean Dif f erence Std. Error
Dif f erence Low er Upper 95% Conf idence
Interval of the Dif f erence t-test f or Equality of Means
