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Penetapan Kadar Fenol Total Ekstrak Etanol Daun Salam yang Diberi Perlakuan Dètente Instantanée Côntrolée (DIC) (Tekanan 1,9 bar dan Waktu
10,86 detik) pada Berbagai Waktu Ekstraksi Priskila Wasti S, 2009
Pembimbing: (I) Indrajati Kohar,(II) Ryanto Budiono
ABSTRAK
Untuk mengetahui waktu ekstraksi optimal daun salam yang diberi perlakuan Dètente Instantanée Côntrolée (DIC) (tekanan 1,9 bar dan waktu 10,86 detik) pada berbagai waktu ekstraksi. Sebelum diukur absorbansinya secara spektrofotometri Vis, total fenol direaksikan dengan pereaksi Folin Ciocalteu. Absorbansi tersebut diukur pada panjang gelombang 757,5 nm dan digunakan asam galat sebagai pembanding. Persentase hasil dari beberapa waktu ekstraksi 5 menit, 15 menit, 30 menit, 1 jam, 1,5 jam, 3 jam, 4 jam, 5 jam, 6 jam, 7 jam, 8 jam, dan 9 jam masing-masing adalah 10.79%, 10.54%, 11.13%, 13.53%, 13.95%, 14.34%, 13.99%, 15.33%, 14.77%, 15.91%, 17.17%, dan 14.52% maka dengan uji statistik ANAVA dan dilanjutkan uji BNT, dapat diketahui bahwa waktu ekstraksi optimal daun salam adalah 8 jam.
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Determination of Total Phenol on Extract Etanol of Polyanthi Folium by Pretreated Dètente Instantanée Côntrolée (DIC) (1,9 bar and 10,86 seconds) at
Various Extraction Time Priskila Wasti S, 2009
Supervisor: (I) Indrajati Kohar,(II) Ryanto Budiono
ABSTRACT
To determine the optimum extraction time of Polyanthi Folium extract by pretreated Dètente Instantanée Côntrolée (DIC) (1,9 bar and 10,86 seconds) at various extraction time has been done. Before the absorbance measured by visible spectrophotometry, phenol total was reacted with Folin Ciocalteu reagent. The absorbance was measured at 757,5 nm and gallic acid solution is used as a standart. The percentages of each extraction time 5 minutes, 15 minutes, 30 minutes, 1 hour, 1,5 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours are 10.79%, 10.54%, 11.13%, 13.53%, 13.95%, 14.34%, 13.99%, 15.33%, 14.77%, 15.91%, 17.17%, dan 14.52% respectedly. It was concluded that the optimum extraction time of Polyanthi Folium was 8 hours, using ANOVA and LSD for statistic al test.