PRODUKSI BIOETANOL DARI AMPAS SAGU
(Metroxylon
Sp)
MELALUI PROSES
PRETREATMENT
DAN METODE
SIMULTANEOUS
SACCHARIFICATION
FERMENTATION
(SSF)
Skripsi Sarjana Kimia
Oleh
KHAIRUNNISAH
BP : 0910413084
JURUSAN KIMIA
FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM
UNIVERSITAS ANDALAS
PRODUKSI BIOETANOL DARI AMPAS SAGU
(Metroxylon
Sp)
MELALUI PROSES
PRETREATMENT
DAN METODE
SIMULTANEOUS
SACCHARIFICATION
FERMENTATION
(SSF)
Oleh
KHAIRUNNISAH
BP : 0910413084
SkripsidiajukanuntukmemperolehgelarSarjanaSains
padaJurusanKimiaFakultasMatematikadanIlmuPengetahuanAlam UniversitasAndalas
JURUSAN KIMIA
FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM
UNIVERSITAS ANDALAS
PADANG
2013
ABSTRACT
BioethanolProductionfromSagoWaste (Metroxylon sp)withPretreatment
ProcessandSimultaneousSaccharificationandFermentation(SSF)Method
By:
Khairunnisah(0910413084)
MarniatiSalim,MS*,ElidaMardiah,MS**
*AdvisorI,**AdvisorII
Sago waste is one of biomass lignocellulose that potential to produce
bioethanol.Lignocellulosematerialconsistofthreemajorcomponentsuchas
cellulose,hemicelluloseandlignin.Theavailableofligninbecomeinhibitorfor
enzymaticprocessitmustremovedbypretreatment.Researchforbioethanol
productionfromsagowastethroughpretreatmentprocessbyusingavariation
ofalkalinesolution;1%NaOH,8%NH4OH,1%NaOH+4%NH4OHand1%
NaOH+8%NH4OH witharatiosolidtoliquid1:10(w/v)afterthat,followedby
SSFmethodinvolvedtwokindoffungilike Trichoderma viride strainT1skfor
saccharificationand Saccharomyces cerevisiae forfermentationprocess.The
optimumconditionsforthereductionofsamplesobtainedafterpretreatmentat
concentrationof1%NaOH+8%NH4OHat49,3017%withalongincubation
period of 3 days with a temperature 500C. Result of determination cellulase
enzymeactivity from Trichoderma viride strainT1skby0.1%CMCis0,1144
unitandthehighestglucoseyieldis921,25µg/mLfrom0,9gramssubstrateof sagowastewhiletheoptimumtimeforthesaccharificationprocessobtainedin
75minutes.EthanolcontentwasanalyzedbyGC/MSresulted12.99%(%area)
orequalwith0,38mLethanolyieldfor168hoursoffermentation.
Keywords: Sagowaste,bioethanol,alkalinepretreatment,SSF
DAFTARISI
HALAMANPENGESAHAN... iii
HALAMANPERNYATAAN.... iv
HALAMANMOTTODANPERSEMBAHAN... v
INTISARI... vii
ABSTRACT.... viii
UCAPANTERIMAKASIH.... ix
DAFTARISI... xi
BABII.TINJAUANPUSTAKA 2.1. TanamanSagu (Metroxylon sp) ... 5
2.2. BiomassaLignoselulosa... 7
2.2.1Hemiselulosa... 7
2.2.2Lignin... 8
2.2.3Selulosa... 9
2.3. Pretreatment lignoselulosa... 9
2.4.ProduksiBioetanoldenganSSF. ... 12
2.5.GC/MS... 15
BABIII.METODOLOGIPENELITIAN... 16
3.3.1LarutanNaOH1%... 17
3.5.4PeremajaanJamur Trichoderma viride StrainT1sk... 19
3.5.5ProduksiEnzimSelulase... 20
3.5.6PenentuanAktivitasEnzimdenganSubstratCMC... 20
3.5.7SakarifikasiEnzimatikVariasiJumlahSubstratAmpasSagu... 20
3.5.8SakarifikasiEnzimatikVariasiLamaSakarifikasiAmpasSagu... 21
3.5.9IsolasidanPemurnian Saccharomyces cerevisiae ... 21
3.5.10PersiapanInokulum Saccharomyces cerevisiae ... 21
3.5.11ProduksiBioetanoldenganSSF... 22
3.5.12PenentuanKonsentrasiEtanol... 22
BABIV.HASILDANPEMBAHASAN 4.1 Pretreatment sampeldenganbasaNaOHdanNH4OH... 23
4.2 PeremajaanJamur Trichoderma viride StrainT1sk... 26
4.3 SakarifikasiEnzimatik... 26
4.3.1HasilAktivitasEnzimdenganSubstratCMC... 27
4.3.3PengaruhLamaSakarifikasiTerhadapKadarGlukosa... 28
4.4 Isolasi Saccharomyces cerevisiae dariFermipan... 29
4.5 BioetanoldenganSSF... 29
BABV.KESIMPULANDANSARAN... 33
5.1 Kesimpulan... 33
5.2 Saran... 33
DAFTARPUSTAKA... 34
LAMPIRAN... 37
