124661410404263 suppl Megga Pikoli

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Bacterial diversity in subbituminous coal and soil

Figure 2: Principal component analysis and biplot representing the soil samples. Only the squared cosines of the variables >0.95 are sh own in the correlation circle.

Figure 4: Diversity indices of Shannon, evenness and dominance among samples with different DNA extraction method. Values in dominance index were multiplied by10x to be shown more clearly.

Suppl.

International Journal of Integrative Biology

_{A journal for biology beyond borders}

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Figure 5:Phylogenetic tree comparing sequence of DGGE bands in are a I. T he area is according to the division shown in Figure 3. T he codes initiated by CO, CS, CF, and CR are those from this study, and the others with their accession number are from GenBank. Hyme nobacter soli was selected as the outgroup species. T he scale bar represents 0.05 substitutions per nucleotide position. Numbers at the nodes are the

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Figure 6:Phylogenetic tree comparing sequence of DGGE bands in area II. T he area is according to the division shown in Figure 3. T he codes initiated by CO, CS, CF, and CR are those from this study, and the others with their accession number are from GenBank. Verrucomicrobium spinosum was selected as the outgroup species. The scale bar represents 0.02 substitutions per nucleotide po sition. Numbers at the nodes are the bootstrap values (%); only values of ≥50% are shown.

Table 1: Description and site coordinate of samples.

S ample Description S ite Coordinate

Coal mixed soil (CS) Coal mined soil S3˚43’44.1”E103˚43’38.1”

Coal seam (CO) Coal seam that has not been mined S3˚44’29.2”E103˚45’11.7”

Coal roof (CR) Horizon of soil on the top of the coal seam S3˚44’29.2”E103˚45’11.7”

Coal floor (CF) Horizon of soil below the coal seam S3˚44’29.2”E103˚45’11.8”

Table 2: Proximate properties of coal samples.

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Figure 7:Phylogenetic tree comparing sequence of DGGE bands in area III. T he area is according to the division shown in Figure 3. T he codes initiated by CO, CS, CF, and CR are those from this study, and the others with their accession number are from GenBank. Gemmata obscuriglobus was selected as the outgroup species. The scale bar represents 0.05 substitutions per nucleotide position. Numbers at the nodes are the bootstrap values (%); only values of ≥50% are shown.

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Table 3: Physical and chemical properties of soil samples.

Characteristics Coal mixed soil (CS ) Coal roof (CR) Coal floor (CF)

Soil category Silty clay Clay Clay

Electrical conductivity (dS/m) 1.64 1.88 0.60

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Table 4: Number of DGGE bands.

Sample Direct method (L) Filtration method (F) Blending method (B)

Coal seam (CO) 35 30 32

Table 5: Similarity of selected sequences to the closest relatives of 16S rRNA gene derived from the respective bands in DGGE gel.

Code of

number Closest sequence in GenBank