KARAKTERISASI ENZIM KITINASE DARI Bacillus sp. BK17, ISOLAT POTENSIAL PENGENDALI HAYATI JAMUR PATOGEN TANAMAN
ABSTRAK
Karakterisasi kitinase yang meliputi pH dan suhu optimum, Km dan Vmax telah dipelajari dari isolat Bacillus sp. BK17. Kitinase kasar dari isolat yang dikultur dalam medium garam minimum dengan kitin koloidal cair selama 5 hari dipresipitasi dengan beberapa konsentrasi amonium sulfat. Aktivitas kitinase tertinggi didapatkan pada presipitasi amonium sulfat 50% dengan aktivitas spesifik sebesar 0,545 Unit. Aktivitas enzim optimum pada pH 6 dan suhu 35 oC yaitu sebesar 0,0750 U/ml. Kitinase Bacillus sp. BK17 memiliki aktivitas sebesar 0,0112 U/ml pada gerusan miselium Sclerotium rolfsii. Enzim ini memiliki Km sebesar 0,46 g dan Vmax sebesar 158,73 g.
Kata kunci: Bacillus sp., Kitinase, Sclerotium rolfsii.
CHARACTERIZATION OF CHITINASE OF Bacillus sp. BK 17, A POTENTIAL ISOLATE FOR CONTROLLING PLANT PATHOGENIC
FUNGI
ABSTRACT
Characterization of chitinase of including pH and temperature, Km and Vmax of Bacillus sp. BK 17 has been conducted. Crude extract of Bacillus sp. BK17 growing in minimum salt medium with colloidal chitin for 5 days was precipited with ammonium sulphate. Optimum chitinase activity was found in 50% ammonium sulphate precipitation with specific activity of 0.545 Units. Chitinase activity in homogenated mycelia of Sclerotium rolfsii was 0.0012 U/ml. The Km and Vmax of the enzyme was 0.46 µg and 158.72 µg, respectively.
Keywords: Bacillus sp. BK 17, Chitinase, Sclerotium rolfsii
