LAPORAN PRAKTIKUM
LAPORAN PRAKTIKUM
MIKROBIOLOGI FARMASI
MIKROBIOLOGI FARMASI
UJI POTENSI ANTIBIOTIK
UJI POTENSI ANTIBIOTIK
ANGGOTA KELOMPOK : ANGGOTA KELOMPOK : E
ETTIIK K PPUUJJI I HHAASSTTUUTTII ((2200114444116666AA)) SORAY
SORAYA PUTRI ORSHITA A PUTRI ORSHITA RESMIRESMI (20144168A)(20144168A) M
MIIRRAAZZIIZZA A AAMMAANNDDAA ((2200114444116699AA)) A
ASSAALLIIA A NNOOPPRRIIYYAANNTTI I MMEERRLLII ((2200114444117700AA))
FAKULT
FAKULTAS
AS FARMASI
FARMASI
UNIVERSITAS SETIA BUDI
UNIVERSITAS SETIA BUDI
SURAKARTA
SURAKARTA
2015
UJI POTENSI ANTIBIOTIK
I. TUJUAN
M!"#"$ %&' **'+,* &-'*"# -.' *'#.#-'#, %*/* "&"##' ''*'+ %#.*%#*/,* %*/* "'*%
II. DASAR TEORI
A*'#.#-'#, %! "*$ ,## ,!" */ %#!"#,* -! -/*#" !#%+&3 '"+, '++** "*$ %* "'+,'+ *-/* */ %#.+' " "#*''#,3 %* % ,% *%! &+ */!.' &-"" &*'#*/ % ,!#%+&* "'+ "&"#" '+ .#! #,--/*#"5 A*'#.#-'#, "+%! .*, %#/+*,* -! ",' +*'+, &*/-.'* ../# &*,#' '+' &*,#' #*,"#5 A,* ''&# ,#.' &,#*
*/ '#%, "#-* %* &,#* */ '#%, '+*'" %# *'##,-. ! %&' .!,* ./# &"#*5 ,'# &*.. &*,#' #*# %&' *%# "#"'*"# '!%& &*/-.'* %*/* *'##,-.5 A*'#.#-'#, %#/+*,* +*'+, */-.'# ../# *#" #*,"# ,#.' ,+* '+ +/ +*'+, &*"# #*,"#3 #"* &% &.%!* ."5
U# &-'*"# *'#.#-'#, " #,-.#--/#, %! "+'+ ',*#, +*'+, *'&,* "+'+ &-'*"# *'#.#-'#, %*/* */+,+ , "*$ '".+' '!%& &'+.+!* #,--/*#" +# */ &, %* ""+#5 E, */ %#'#.+,* &%
"*$ +# %&' .+& !.'* &'+.+!*5
A*'#.#-'#, %! ';' ,## */ %#!"#,* #,-;-/*#" !#%+& '+' +*/# %* .,'# '*!3 */ ##,# ,!"#' '#,* '+ */!.' &'+.+!* .*, .,'# %* ..& #+" ."3 "%*/,* '-,"#"#'"* ./#
*+"# '# ,#5
K/#'* *'#.#-'#, +*'+, &' ,#* %#'+,* -! "* I*//#" %5A<*% =#*/ '!+* 1928 (&*#"##*)5 T'&# &*+* #*# .+ %# &,.*/,* %* %#&/+*,* % '&# %# '!+* 1941 -! %5=- (O<-%)5 K+%#* .*, ' #* %*/* ,!"#' *'#.#-'#, %##"-# -! &*#%#,;&*#%#, %#
"++! %+*#3 ,* ''&# .!+.+*/ %*/* "#' '-,"#"* !* ..& " */ %&' %#/+*,* "./# -.'5 P'+.+!* %* &*/"* .,'#;.,'# %#&*/+!# -! ../# ' ,## % #*/,+*/* ,* &*/+! ' ,##3 , .,'# "&'# ./, *++ '+ *+!# ' ,## #'+5 P#"'#$5 # .,'#;.,'# #'+ ''#, %* ./, *++ ,! ' ,## ,#' ".+' !-'<#" (>) %* ".#,* ,#' ".+' !-'<#" (;)5 ,'#;.,'# */ '#%, ./,3 &'+.+!* ,--*#* %&' %#&*/+!# -! ';' ,##. &#"'#$ #'+ %#".+' !-'-&#" 5
S+'+ ' *'##,-. */ #% ##,# '-,"#"#'" ",'#5 I"'#! #*# .'# .!$ "+'+ -.' ..! ./# &"#' ''&# '#%, .!,* #**/5 S#*/,#3 '-,"#"#'" ",'# .#! ."#' '# %* .+,* ."-+'? #*# .'# .!$ "+'+ -.' */ &% ,-*"*'"# ''*'+ %&' %#'-*"# -! #**/3 %&' +", &"#' 5
A*'#.#-'#, */ #% "./# -.' !+" *+!# "' ; "' .#,+' :
15 M&+*# ,&+* +*'+, '#,* '+ */!.' &'+.+!* #,--/*#" */ +" (.-% "&'+ *'#.#-'#)5
25 T#%, *#.+,* '%#* "#"'*"# %# #,--/*#" &'!-/*
@5 T#%, *#.+,* &*/+! "&#*/ ("#% ') */ .++, &% !-"'3 "&'# ,"# /#3 ,+",* "3 ##'"# .+*/3 %* "./#*
45 T#%, *//*//+ ,"#.*/* - */ *- %# !-"' "&'# - +"+" '+ - ,+#'5
III. CARA KERJA
15 M.+' .5 25 M*/#*-,+"# .,'# " &'* %*/* ,&" #%#5 @5 M" &% %#*%#*/ '.+*/ %*/* %#',*5 45 M*+*//+ ,+*/ .#! *#' / .,'# .%#+"# &% %#+ /5 5 M+.*/# %# %*/* .-- &-& ".*, 6 +.*/5 65 M"+,,* *'#.#-'#, &% "'#& +.*/ ""+# ** ".*, 0B5 75 M*/#*,+."# " 24;48 %*/* "+!+ @70C5
IV. DATA PERCOBAAN & PERHITUNGAN 5 D' &-.*
.5 P!#'+*/*
S& S&1 > S&2 > S&@ 1620 > 180 > 2080 0
S' S'1 > S'2 > S'@ 1770 > 1990 > 2180 2940
LS& S&@ F S&1 2080 F 1620 460 LS' S'@ F S'1 2180 F 1770 410
.
LSp+ LSt(
d−1)
. I . n . h
460+410(
3−1)
.0,3010.7 .2
10@3 2@ S& ∑ sp n . d 5550 7.3 264328 S'
∑ St n . d
5940 7.3 28238 L-/ &-'*"# '#- -.' R ySp−
ySt b
264,28−282,85 103,23NO S& 1 S& 2 S& @ S' 1 S' 2 S' @
1 2@0 240 290 280 290 @10 2 210 20 @00 280 280 @20 @ 270 280 @20 280 @10 @20 4 2@0 280 @00 210 280 290 2@0 20 280 210 260 @10 6 200 20 270 220 270 @00 7 20 @00 @20 290 @00 @@0 1620 180 2080 1770 1990 2180
0318 P-'*"# A*'#.#-'#, A*"'# -/ R 0366 < 100 66 K'*/* % +! &*/** * +! &#,+"# */ %#/+*,* I -/ '#*/,' %-"#" ! +! -.' */ %#/+*,* V.
VI. PEMBAHASAN
P% &-.* ,# #*# %#,+,* &**#'#* &-'*"# *'#.#-'#,5P-.* #*# .'++* +*'+, **'+,* ."* &-'*"# *'#.#-'#, "& '!%& *'#.#-'#,
"'*%5 S+'+ *'#.#-'#, +,* ,-*"*'"# ''*'+ / %&' **,* +*/"#* #'+ "./# .,'#-"''#, '+ .,'#-"#%5 P-'*"# */ %#.#,* *++' ,-& !+"! 9 ; 103 %# + #'+ .'# *'#.#-'#, "& '#%, *+!# "' +*'+, %&' %#%,* %# &"*5
P% &-.* ,# #*#3 '-% */ %#/+*,* % &**'+* &-'*"# *'#.#-'#, %! '-% &*'&* %*/* &*/ "##*% #'+ *//+*,* &-'- +*'+, */+# *'#.#-'#, &% %# *+'#* / */ .#"# #*-,++ .,'# &% $* &'#5 P-'*"# %&' %#'*'+,* %*/* */+,+ -* .*#*/ */ %#!"#,* %* .*%#*/,** %*/* %#' -* .*#*/ %# *'#.#-'#, "'*% .,+5
S' &*//+** .#,* .,'# */ %#&,# %! !+" .#,* +*# (&+ "'#%)5 M,"+% %# .#,* +*# %! .,'# */ %#.# %# " */"+*/ ,+%#* %#.#,,*3 .+,* %# .,'# */ %##"-"# %# .-'-#+ ,#*#" ("& %!3 ""3 +#*3 %* "./#*)5
P% &-.* #*# *'#.#-'#, */ %#/+*,* %! ,-*#,- 8 %* "+"&*"# .,'#* %! Escherichia coli ,* *++' ,-& %* #''+ */ % *'#.#-'#, ,-*#,- "*"#'# '!%& .,'# Escherichia coli.
P-.* %#$# %*/* *#&,* ' %* .!* */ %#.+'+!,* "&'# "+"&*"# *'#.#-'#, "& %* "'*%3 NA */ '! %#&+ "+"&*"# .,'#3 '.+*/ ,"#3 &-'-3 '.+*/,"#%*&#&' */ "+%!%#"'##""#'.#!%!++ %# %+'-,3 #*# %#,+,* +*'+, */!#*%,* ' %# ,-*'#** #* */ %&' *//*//+ % &-.*5 T.+*/,"#%#,#*/,* %# % -*3 ''&# -+ &#&' '#%,.-!%#,#*/,*%#% -* ,* -+ &#&' +&,*' */ &+*#",3 #,%#,#*/,*%*/*&*"* , '.+*/ ,*+# "!#*// ", '#%, ,+' /#5
%#,+,* &./#* &% &+,* %" $* &'# *%# 6 " ."5 S'#& #*# %#.# . +'* "& '#*//# (S') %* *%! (S&) +*'+,
&+%! % &*/'*5 P% &*//+** $* &'#3 */* %#.#,* % ,-*%#"# '.+,3 / #"# $* '#%, ',-*'#*"# -! +% +5
S+ '!& &*/* &-"%+ !+" %#,+,* " "&'#"3 ! #*# %#,+,* +*'+, */!#*%# ,-*'#*"# */ '%# -! #,-. #* */ %&' +", &-.*5 S'! "+"&*"# .,'# %#&+,* %*/* *+'#* /%* %#%#,* !#*// .,+5 P-"" &.+'* +.*/ !+" %#,+,* %*/* &' %*/* *//+*,* &-'-3 */* .#,* $* &'# '.+, '+ +*'+, */!#*%# .,'# %# + "+, , % $*5 S'! ,* %! */ %#./# '%# '! %#+.*/#3 , %#"+,,*! +'* *'#.#-'#, %*/* %-"#" *%!3 **/! %* '#*//# %# +'* .,+ +&+* +'* "&5 M"#*/ F "#*/ +.*/ %##"#*'#.#-'#, %*/* *//+*,* #,-&#&'5 M#,-&#&' */ %#/+*,* !+"! ."#!
P*/#"#* *'#.#-'#, %#,+,* %# %,'  / ''& "&'#"5 T'&# */* "&# '#& &% #,-&#&' #,+' '., ,* ,* &*/+!# +! +'* */ %#.#5 P*/#"#* *'#.#-'#, +/ !+" %*/* !'#;!'#3 */* "&# *'#.#-'#, ,+ %# +.*/ "-# / !"# */ %#&-! ,"#5 P% "' *'",* *'#.#-'#, !+" '&' &% +.*/3 %* +.*/ */ %#.*'+, !+" .+' / *'#.#-'#, .%#+"# "&+* %* -* */ %#!"#,* +/ .+' (%#' */ %#!#'+*/ +%!)5
S'! "+ +.*/ '#"#3 $* &'# !+" %#.+*/,+" %*/* ,-* % ,%* '#%, %#.#,3 ,+%#* %##*,+."#,* &% "+!+ @70C */ +&,* "+!+ -&'#+
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
D* %# %' %# &-! ." &-'*"# *'#.#-'#, ,-*#,- #*# *++' #''+ &% ,-& bel! l"#"$ +*'+, %#+ %# &"* ,* &-'*"#* ,+*/ %# "' */ .,+ #'+ 9;12
VII. KESIMPULAN
15 U# P-'*"# *'#.#-'#, K!-*#- "." 66
25 U# &-'*"# *'#.#-'#, ,!-*#- %#.$! "'*% #'+ ,+*/ %# 9;125
VIII. DAFTAR PUSTAKA
P'#$#3T520083 Mikrobiologi Farmasi5 J,': E*//
H%#-'--3R5S51985M#,-.#--/# D" % P,',: PT5 G%#5
