Penetapan Kadar Asam Benzoat Dalam Sediaan Sirup Multivitamin Secara Spektrofotometri Ultraviolet

29

Lampiran 1

Hasil Kromatografi Lapis Tipis

30

Spectrophotometric Report

Data information

File Name: C:\Documents and Settings\ Administrator\My Documents\ OT\Pengawet\Bk. Benzoat 1.pho Analyst: System Administrator

Date/Time: 03/01/2012 12:00 PM

Instruments Information Instrument Name: Instrument 1 Instrument Type: UV-1800 Series

Spetrum

Standard Table

No Sample ID Type Conc WL225.0 Wgt.Factor Comments

1 Bk. Benzoat 1 Standard 0.2000 0.4392 1.000 1 ad 10 5 ad 10

Sample Table

No Sample ID Type Conc WL225.0 Comments

31

Lampiran 2

Hasil Spektrofotometri Ultraviolet

Lampiran 3

Perhitungan Hasil Kromatografi Lapis Tipis

Rumus:

Harga Rf =

Harga Rf :

Baku Asam Benzoat =

= 0,57

Sampel Sirup

=

= 0,52

32

Lampiran 4

Perhitungan Kadar Asam Benzoat dalam Sirup Multivitamin

Kadar Asam Benzoat 1

Data

Au

: 0,5273

Ab

: 0,4392

Kb

: 2 mg/ml

Vtb

: 50 µl

Vtu

: 200 µl

Veu

: 5 ml

B

: 25046,5 mg

F

: 1

Kadar Baku

: 99,94%

Kadar asam benzoat dapat dihitung:

Kadar

=

x

Kb

x

x F x Kadar Baku

Kadar

=

x

2

x

x 1 x 99,94%

Kadar

= 0,01197%