Penggunaan 2 Suhu Sentrifuse yang Berbeda dan Phenol Red pada Sampel Buffy Coat dalam Perhitungan Jumlah CD3 - CD4.

(1)

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  

















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 

 

 

 

 

  

  

 

   



    



   

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 

utuh (Kelompok[K]-1], BC dengan phenol red dan nonrefrigerated sentrifuge (K-2), BC non phenol red – non refrigerated sentrifuge (K-3) , dan BC non phenol red – refrigerated sentrifuge (K-4). Replikasi dilakukan 3 kali. Rata – rata jumlah CD3 – CD4 (sel /l) adalah 1382- 544(K-1), 110 – 46 (K-2), 2578 -1147(K-3), dan 2601 -1208.



red (K-2) dibandingkan dengan sampel yang tidak menggunakan phenol red (K –3). Hal ini tidak terjadi pada penggunaan suhu sentrifuge yang berbeda (K –3 dan K-4).



BC non phenol red – non refrigerated centrifuge (G-3) , and BC non phenol red – refrigerated centrifuge (G-4). Triple replication was applied on each procedure. Mean values of CD3 – CD4 count (cell / 110 – 46 (G-2), 2578 -1147(G-3), and 2601 -1208. (G-4). On this study, there was a big difference on CD3-CD4 count between samples with phenol red (G-2) compared to phenol free samples (G –3), but it was not on samples prepared with two different centrifuge temperatures (G –3 and G-4).



 

   

Warna merah pada pH 7.2 – 7.4 dapat berubah menjadi kuning atau

    

(7)

  

Metode penelitian observasional ini telah disetujui oleh Komite Etik Rumah Sakit Sanglah – Fakultas



Besar Veteriner (BBVet) pada periode 1 May – 31 Juli, 2015.

 

Hasil perhitungan jumlah CD3 – CD4 ditampilkan pada Tabel 1.

(8)

 

phenol red (K-2) dibandingkan dengan sampel yang tidak menggunakan phenol red (K –3). Hal ini tidak

terjadi pada penggunaan suhu sentrifuge yang berbeda (K –3 dan K-4). Media tanpa

  



Riset ini merupakan bagian penelitian dari Hibah Pekerti – Desentralisasi 2015, yang didanai oleh DIKTI – Universitas Udayana.



Hulspas R., O’Gorman M.R.G., Wood B.L., Gratama J.W., and Sutherland D.R.(2009). Considerations



Cytometry Part B (Clinical Cytometry) 76B:355–364.

 



Effects Caused By Centrifugation. Journal Of Engineering Studies And Research – Volume 20 No.

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  

                      



 

                          

 

