PENGARUH EKSTRAK PHALERIA MACROCARPA TERHADAP INDEK MITOSIS SEL DAN PERTUMBUHAN DIAMETER KARSINOMA EPIDERMOID - Diponegoro University | Institutional Repository (UNDIP-IR)

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76

LAMPIRAN 1

Gambar / foto sel mitosis pada jaringan karsinoma epidermoid (anak panah merah

dengan perbesaran 400x)

Kelompok kontrol

Kelompok P1

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77

LAMPIRAN 2

Analisa Statistik Perubahan Diameter Tumor

Case Summaries

Selisih diameter tumor

3 1.1000 .26458 1.0000 .90 1.40

3 .4333 .25166 .4000 .20 .70

3 .4000 .10000 .4000 .30 .50

3 -.2667 .20817 -.2000 -.50 -.10

12 .4167 .53739 .4000 -.50 1.40

Kelompok

N Mean Std. Deviation Median Minimum Maximum

Case Summaries

3 3

1.3000 2.4000 1.47986 1.34536 .6000 2.0000

.30 1.30

3.00 3.90

3 3

.5667 1.0000 .55076 .78102

.3000 .6000

.20 .50

1.20 1.90

3 3

1.1000 1.5000 1.47986 1.57162

.4000 .8000

.10 .40

2.80 3.30

3 3

.9667 .7000

1.15902 .95394

.4000 .2000

.20 .10

2.30 1.80

12 12

.9833 1.4000 1.08362 1.22771 .4000 1.0500

.10 .10

3.00 3.90

N Mean

Std. Deviation Median Minimum Maximum N

Mean

Std. Deviation Median tumor pre test

Diameter tumor post

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78

NPar Tests Kruskal-Wallis Test

Tests of Normality

.314 3 . .893 3 .363

.219 3 . .987 3 .780

.175 3 . 1.000 3 1.000

.292 3 . .923 3 .463

Kelompok K P1 P2 P3 Selisih diameter tumor

Statistic df Sig. Statistic df Sig. Kolmogorov-Smirnova Shapiro-Wilk

Lilliefors Significance Correction a.

Test of Homogeneity of Variances

1.129 3 8 .394

Levene

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79

Mann Whitney Test

Test Statisticsb

.000 6.000 -1.964 .049

.100a Mann-Whitney U

Wilcoxon W Z

Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]

Tumor diameter diference

Not corrected for ties. a.

Grouping Variable: Group b.

.000 6.000 -1.964 .049

Wilcoxon W Z

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80

.000 6.000 -1.964 .049

Wilcoxon W Z

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81

.000 6.000 -1.964 .049

Wilcoxon W Z

Grouping Variable: Group b.

.000 6.000 -1.964 .049

Wilcoxon W Z

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82

LAMPIRAN 3

Analisa Statistik Indek Mitosis

NPar Tests

Kruskal-Wallis Test

Case Summaries

Index mitosis

3 3.2000 .34641 3.4000 2.80 3.40

3 1.1333 .11547 1.2000 1.00 1.20

3 1.0667 .11547 1.0000 1.00 1.20

3 .6667 .11547 .6000 .60 .80

12 1.5167 1.04606 1.1000 .60 3.40

Kelompok

N Mean Std. Deviation Median Minimum Maximum

.385 3 . .750 3 .000 Index mitosis

Statistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova Shapiro-Wilk

Test of Homogeneity of Variances

Index mitosis

5.333 3 8 .026

Levene

Statistic df1 df2 Sig.

Ranks Index mitosis

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83

NPar Tests

Mann-Whitney Test

NPar Tests

Mann-Whitney Test

Test Statisticsa,b

9.804 3 .020 Chi-Square

df

Asymp. Sig.

Index mitosis

Kruskal Wallis Test a.

Grouping Variable: Kelompok b. Index mitosis

N Mean Rank Sum of Ranks

.000 6.000 -2.023 .043 .100a Mann-Whitney U

Wilcoxon W Z

Index mitosis

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84

NPar Tests

Mann-Whitney Test

NPar Tests

Mann-Whitney Test

.000 6.000 -2.023 .043 .100a Mann-Whitney U

Wilcoxon W Z

Index mitosis

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85

NPar Tests

Mann-Whitney Test

3.000 9.000 -.745 .456 .700a Mann-Whitney U

Wilcoxon W Z

Index mitosis

.000 6.000 -2.023 .043 .100a Mann-Whitney U

Wilcoxon W Z

Index mitosis

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86

NPar Tests

Mann-Whitney Test

.000 6.000 -2.023 .043 .100a Mann-Whitney U

Wilcoxon W Z

Index mitosis

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87

LAMPIRAN 4

Uji Korelasi Selisih Diameter Tumor dan Indek Mitosis

Explore

Nonparametric Correlations

Case Summaries

12 12

.4167 1.5167

.53739 1.04606

.4000 1.1000

-.50 .60

1.40 3.40

N Mean

Std. Deviation Median Minimum Maximum

Selisih diameter

tumor Index mitosis

.105 12 .200* .988 12 .999

.369 12 .000 .742 12 .002

Selisih diameter tumor Index mitosis

Statistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova Shapiro-W ilk

This is a lower bound of the true significance. *.

Correlations

1.000 .813**

. .001

12 12

.813** 1.000

.001 .

12 12

Correlation Coefficient Sig. (2-tailed) N

Index mitosis Spearman's rho

Selisih diameter

tumor Index mitosis

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88

LAMPIRAN 5

Patologi Anatomi Sampel, Ukuran Diameter Tumor Pre dan Post Perlakuan, Indek

Mitosis Post Perlakuan

SCORING INDEX M ITOSIS DALAM 5 LAPANG

PANDANG BESAR HASIL PATOLOGI ANATOM I

KELOM POK I II III IV V M ean

Kont rol

A 4 3 3 5 2 3.4 SQUAM OUS CELL CA WELL

DIFFERENTIATED

B 2 3 4 3 2 2.8 SQUAM OUS CELL CA Insit u

C 4 3 2 3 5 3.4 SQUAM OUS CELL CA Insit u

m ean 3.2

P1

A 1 2 1 1 1 1.2

KERATOACANTOM A DENGAN SQUAM OUS CELL CA

M IKROINVASIF

B 0 1 2 1 1 1 PAPILOM A CELL SQUAM OUS

DENGAN FOKUS CA

C 3 1 1 1 0 1.2 SQUAM OUS CELL CA Insit u

M ean 3.4

P2

A 1 1 1 0 2 1 SQUAM OUS CELL CA WELL

DIFF DENGAN NEKROSIS LUAS

B 2 2 0 1 1 1.2 SQUAM OUS CELL CA WELL

DIFFERENTIATED

C 1 0 2 2 0 1 SQUAM OUS CELL CA WELL

DIFFERENTIATED

m ean 1.06

P3

A 0 1 1 2 0 0.8 SQUAM OUS CELL CA WELL

DIFFERENTIATED

B 1 0 0 1 1 0.6 SQUAM OUS CELL CA Insit u

C 1 1 0 1 0 0.6 SQUAM OUS CELL CA INSITU +

M IKROINVASIF

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89

Data Ukuran Diameter Tumor Pre dan Post Perlakuan

Kelompok

Diameter

tumor pre test

(cm)

Diameter

tumor post

test (cm)

Selisih diameter

tumor (cm)

Kontrol

A

3 3,9

0,9

Keterangan :

K

: Kontrol

P1

: Phaleria macrocarpa 0,0715 mg (0,36 ml/hari).

P2

: sitostatika Paclitaxel 175 mg/m

2

dan cisplatin 50 mg/m

2

P3

: sitostatika Paclitaxel 175 mg/m

2

dan cisplatin 50 mg/m

2

dan dikombinasikan

dengan Phaleria macrocarpa 0,0715 mg (0,36 ml/hari)

A

: tanda asam pikrat pada mencit di kepala

B

: tanda asam pikrat pada mencit di kaki depan

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90

LAMPIRAN 6

Foto Mencit yang Diinduksi Tumor, Perlakuan, dan Pengukuran Tumor

Gambar. Mencit yang diinduksi

karsinoma epidermoid

Gambar. Pemberian Phaleria macrocarpa

via sonde

Gambar. Penyuntikan sitostatika

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91

LAMPIRAN 7

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92

LAMPIRAN 8

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