Pengaruh Interesterifikasi pada Lemak Sapi dan Minyak Kelapa Sawit terhadap Profil Lipida Marmut

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Lampiran 1. Bagan Alur Prosedur Interesterifikasi Kimia

150 ml sampel

Ditambah 15 ml NaOCH

3

0,1N

Diaduk dengan kecepatan 4000 rpm

pada suhu 60-70

o

C selama variasi

waktu (30,60,90,120 menit)

Hasil reaksi

Ditambah asam Sitrat 20% (b/v)

Dimasukkan dalam corong pisah

Ditambah 150 ml n-heksan

Dicuci dengan akuades 3 (tiga) kali

Lapisan atas

Ditambah Na

2

SO

4

anhidrat

Disaring

dirotarievaporasi

Ditentukan titik leburnya

Larutan siap diuji

pada marmut

Lampiran 2. Bagan Alur Penentuan Titik Lebur

Dimasukkan dalam pipa kapiler Ø 1 mm

Dibekukan selama 24 jam dalam

freezer

Titik lebur sampel

Dimasukkan dalam alat Melting

Point Aparatus

Dinaikkan suhu pada alat secara

bertahap & perlahan hingga meleleh

Dicatat titik leburnya

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Lampiran 3.

Bagan Alur Pengujian Efek terhadap Profil Lipida Serum Darah

Marmut

Marmut

Kadar Profil

Lipida Awal

Dikondisikan selama 2 minggu

Diukur serum

d

h

Diberi perlakuan selama

21 hari

Diukur kadar profil

lipida pada hari ke-1,7

dan 14

Lampiran 4. Bagan Alur Pengambilan Serum Darah Marmut

Marmut

Dipuasakan selama 10-14 jam

Dipangkas bulu kakinya

Disikat kuku kakinya dengan

sikat gigi basah

Kuku yang bersih

Dipotong dengan pemotong

kuku sampai berdarah

Dibilas dengan alkohol

Darah marmut

Didiamkan selama 10 menit

Disentrifugasi dengan kecepatan

3000 rpm selama 15 menit

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Lampiran 5. Bagan Alur Pengukuran Kadar Kolesterol

Dipipet sebanyak 10 µl

Dimasukkan dalam tabung reaksi

yang berisi larutan reagensia

kolesterol sebanyak 1 ml

Kadar kolesterol total

Serum

Dihomogenkan

Diinkubasi pada suhu kamar

selama 20 menit

Diukur pada mikrolab 300 dengan

panjang gelombang 546 nm

Lampiran 6. Bagan Alur Pengukuran Kadar Trigliserida

Dipipet sebanyak 10 µl

Dimasukkan dalam tabung reaksi

yang berisi larutan reagensia

trigliserida sebanyak 1 ml

Kadar trigliserida

Serum

Dihomogenkan

Diinkubasi pada suhu kamar

selama 20 menit

Diukur pada mikrolab 300 dengan

panjang gelombang 546 nm

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Lampiran 7. Bagan Alur Pengukuran Kadar HDL

Dipipet sebanyak 100 µl

Dimasukkan dalam tabung reaksi

yang berisi larutan reagensia

kolesterol HDL sebanyak 1 ml

Kadar HDL

Serum

Dihomogenkan

Diinkubasi pada suhu kamar

selama 20 menit

Diukur pada mikrolab 300 dengan

panjang gelombang 546 nm

Lampiran 8. Data Kadar Kolesterol Total Serum Darah Marmut (mg/dl)

Perlakuan

Berat

Badan

(gram)

Kadar Kolesterol (mg/dl)

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Lampiran 9. Data Kadar Trigliserida Serum Darah Marmut (mg/dl)

Perlakuan

Berat

Badan

(gram)

Kadar Trigliserida (mg/dl)

Lampiran 10. Data Kadar HDL Serum Darah Marmut (mg/dl)

Perlakuan

Berat

Badan

(gram)

Kadar HDL (mg/dl)

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Lampiran 11. Data Kadar LDL Serum Darah Marmut

Perlakuan

Berat

Badan

(gram)

Kadar LDL (mg/dl)

Lampiran 12. Analisis Statistik Kolesterol Total Pemberian Lemak Sapi

Univariate Analysis of Variance

Dependent Variable:Hasil Pengamatan

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Lanjutan Lampiran 12.

Tests of Between-Subjects Effects Dependent Variable:Hasil

Pengamatan

Partial Eta Squared

Model 20983.413

a

24 874.309 107.32

1 .000 .981 2575.705 1.000 Intercept 331735.25

3 1

15483.013 4 3870.753 475.13

3 .000 .974 1900.534 1.000 Menit_Ke *

Hari_Ke 4897.387 16 306.087 37.572 .000 .923 601.152 1.000

Error 407.333 50 8.147

Total 353126.00

0 75 Corrected

Total 21390.747 74

a. R Squared = .981 (Adjusted R Squared = .972)

Post Hoc Tests

Lama Reaksi (menit)

Multiple Comparisons Hasil Pengamatan

Tukey HSD

Mean Difference

(I-J) Std. Error Sig.

95% Confidence Interval

Lower Bound Upper Bound

Lanjutan Lampiran 12

Based on observed means.

The error term is Mean Square(Error) = 8.147. *. The mean difference is significant at the .05 level.

Homogeneous Subsets

Hasil Pengamatan

Means for groups in homogeneous subsets are displayed.

Based on observed means.

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Lampiran 13. Analisis Statistik Trigliserida Pemberian Lemak Sapi

Univariate Analysis of Variance

Dependent Variable:Hasil Pengamatan

Lanjutan Lampiran 13.

Tests of Between-Subjects Effects Dependent Variable:Hasil

Pengamatan

Partial Eta Squared

Model 22195.813

a

24 924.826 113.522 .000 .982 2724.527 1.000

Intercept

337747.853 1 337747.853 4.146E

4 .000 .999 41458.411 1.000

Corrected Total _{22603.147 74}

Post Hoc Tests

Lama Reaksi (menit)

Multiple Comparisons Hasil Pengamatan

Tukey HSD

Mean Difference

(I-J) Std. Error Sig.

95% Confidence Interval

Lower Bound Upper Bound

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Lanjutan Lampiran 13.

90 0 6.4000* 1.04222 .000 3.4507 9.3493

Based on observed means.

The error term is Mean Square(Error) = 8.147. *. The mean difference is significant at the .05 level.

Homogeneous Subsets

Hasil Pengamatan

Means for groups in homogeneous subsets are displayed.

Based on observed means.

Lampiran 14. Analisis Statistik HDL Pemberian Lemak Sapi

Univariate Analysis of Variance

Descriptive Statistics Dependent Variable:Hasil

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Lanjutan Lampiran 14.

Tests of Between-Subjects Effects

Dependent Variable:Hasil

Source

Type III Sum

of Squares df Mean Square F Sig.

Partial Eta

Squared

Corrected Total 480.667 74

a. R Squared = .330 (Adjusted R Squared = .009)

Post Hoc Tests

Lama Reaksi (Menit)

Multiple Comparisons

Mean Difference

(I-J) Std. Error Sig.

95% Confidence Interval

Lower Bound Upper Bound

Lanjutan Lampiran 14.

Based on observed means.

The error term is Mean Square(Error) = 6.440. *. The mean difference is significant at the .05 level.

Homogeneous Subsets

Means for groups in homogeneous subsets are displayed.

Based on observed means.

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Lampiran 15. Analisis Statistik LDL Pemberian Lemak Sapi

Univariate Analysis of Variance

Descriptive Statistics Dependent Variable:Hasil

Lanjutan Lampiran 15.

Tests of Between-Subjects Effects

Dependent Variable:Hasil

Source

Type III Sum

of Squares df Mean Square F Sig.

Partial Eta Squared

Corrected Model _13606.441a

24 566.935 66.725 .000 .970

Corrected Total _{14031.267 74}

a. R Squared = .970 (Adjusted R Squared = .955)

Post Hoc Tests

Lama Reaksi (Menit)

Multiple Comparisons

Mean Difference

(I-J) Std. Error Sig.

95% Confidence Interval

Lower Bound Upper Bound

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Lanjutan Lampiran 15.

120 0 3.6400* 1.06436 .011 .6281 6.6519

30 6.1733* 1.06436 .000 3.1614 9.1853

60 1.1067 1.06436 .836 -1.9053 4.1186

90 1.9867 1.06436 .348 -1.0253 4.9986

Based on observed means.

The error term is Mean Square(Error) = 8.497. *. The mean difference is significant at the .05 level.

Homogeneous Subsets

Hasil

Means for groups in homogeneous subsets are displayed. Based on observed means.

Lampiran 16. Analisis Statistik Kolesterol Pemberian Minyak Kelapa Sawit

Univariate Analysis of Variance

Descriptive Statistics Dependent Variable:Hasil Pengamatan

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Lanjutan Lampiran 16.

Tests of Between-Subjects Effects Dependent Variable:Hasil

Pengamatan

Partial Eta Squared

Model 8252.320

a

24 343.847 36.736 .000 .946 881.658 1.000

Intercept 189907.68

0 1

Total 198628.00

0 75 Corrected

Total 8720.320 74

a. R Squared = .946 (Adjusted R Squared = .921)

Post Hoc Tests

Lama Reaksi (menit)

Multiple Comparisons Hasil Pengamatan

Tukey HSD

Mean Difference

(I-J) Std. Error Sig.

95% Confidence Interval

Lower Bound Upper Bound

Lanjutan Lampiran 16.

Based on observed means.

The error term is Mean Square(Error) = 9.360. *. The mean difference is significant at the .05 level.

Homogeneous Subsets

Hasil Pengamatan

Means for groups in homogeneous subsets are displayed. Based on observed means.

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Lampiran 17. Analisis Statistik Trigliserida Pemberian Minyak Kelapa Sawit

Univariate Analysis of Variance

Descriptive Statistics Dependent Variable:Hasil Pengamatan

Lanjutan Lampiran 17.

Tests of Between-Subjects Effects

Dependent Variable:Hasil

Pengamatan

Partial Eta

Squared

Model 11225.520

a

24 467.730 49.971 .000 .960 1199.308 1.000

Intercept

194463.480 1 194463.48

0 2.078E4 .000 .998 20776.013 1.000

b. Computed using alpha = .05

Post Hoc Tests

Lama Reaksi (menit)

Multiple Comparisons Hasil Pengamatan

Tukey HSD

Mean Difference

(I-J) Std. Error Sig.

95% Confidence Interval

Lower Bound Upper Bound

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Lanjutan Lampiran 17.

60 0 6.3333* 1.11714 .000 3.1720 9.4946

Based on observed means.

The error term is Mean Square(Error) = 9.360. *. The mean difference is significant at the .05 level.

Homogeneous Subsets

Means for groups in homogeneous subsets are displayed. Based on observed means.

Lampiran 18. Analisis Statistik HDL Pemberian Minyak Kelapa Sawit

Univariate Analysis of Variance

Dependent Variable:Hasil

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Lanjutan Lampiran 18.

Tests of Between-Subjects Effects

Dependent Variable:Hasil

Source

Type III Sum

of Squares df Mean Square F Sig.

Partial Eta

Squared

Post Hoc Tests

Lama Reaksi (Menit)

Multiple Comparisons

Mean Difference

(I-J) Std. Error Sig.

95% Confidence Interval

Lower Bound Upper Bound

Lanjutan Lampiran 18.

120 0 -4.3333* .75542 .000 -6.4710 -2.1956

30 -1.7333 .75542 .164 -3.8710 .4044

60 -.0667 .75542 1.000 -2.2044 2.0710

90 .7333 .75542 .867 -1.4044 2.8710

Based on observed means.

The error term is Mean Square(Error) = 4.280. *. The mean difference is significant at the .05 level.

Homogeneous Subsets

Hasil

Means for groups in homogeneous subsets are displayed. Based on observed means.

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Lampiran 19. Analisis Statistik LDL Pemberian Minyak Kelapa Sawit

Univariate Analysis of Variance

Descriptive Statistics Dependent Variable:Hasil

Lanjutan Lampiran 19.

Tests of Between-Subjects Effects

Dependent Variable:Hasil

Source

Type III Sum

of Squares df Mean Square F Sig.

Partial Eta

Squared

Corrected Total 9183.911 74

a. R Squared = .944 (Adjusted R Squared = .917)

Post Hoc Tests

Lama Reaksi (Menit)

Multiple Comparisons

Mean Difference

(I-J) Std. Error Sig.

95% Confidence Interval

Lower Bound Upper Bound

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Lanjutan Lampiran 19.

90 0 9.7067* 1.17203 .000 6.3900 13.0233

Based on observed means.

The error term is Mean Square(Error) = 10.302. *. The mean difference is significant at the .05 level.

Homogeneous Subsets

Hasil

Means for groups in homogeneous subsets are displayed. Based on observed means.

Lampiran 20. Pengujian Beda Nilai Rata-Rata Kadar Kolesterol Total Setelah

Pemberian Lemak Sapi dan Minyak Kelapa Sawit Hasil

Interesterifikasi

Hari ke-

Rata-rata Kadar (n=3) Kolesterol total (mg/dl)

Lemak Sapi

Minyak Kelapa Sawit

0

44.67

44.00

Kedua sampel adalah independen, maka dilakukan uji t beda antara dua mean dengan

taraf kepercayaan 95% untuk mengetahui apakah rata-rata kedua sampel

sama (σ

1

=

σ

2

)

atau berbeda (σ

1

≠ σ

2

)

2.

Nilai kritis t yang diperoleh dari tabel (t

2

0,05/2

Daerah kritis penerimaan : -2,3060

≤ t

(8))adalah = 2,3060

o

Daerah kritis penolakan : t

≤

2,3060

o

< -2,3060 dan t

o

3.

T hitung antara dua mean

> 2,3060

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Lanjutan Lampiran 20.

4.

Karena t

o

= 2.81 > 2.3060, maka hipotesa H

o

ditolak. Berarti terdapat

Lampiran 21. Pengujian Beda Nilai Rata-Rata Kadar Trigliserida Setelah Pemberian

Lemak Sapi dan Minyak Kelapa Sawit Hasil Interesterifikasi

Hari ke-

Rata-rata Kadar (n=3) Trigliserida (mg/dl)

Lemak Sapi

Minyak Kelapa Sawit

0

48.67

48.00

Kedua sampel adalah independen, maka dilakukan uji t beda antara dua mean dengan

taraf kepercayaan 95% untuk mengetahui apakah rata-rata kedua sampel

sama (σ

1

=

σ

2

)

atau berbeda (σ

1

≠ σ

2

)

2.

Nilai kritis t yang diperoleh dari tabel (t

2

0,05/2

Daerah kritis penerimaan : -2,3060

≤ t

(8))adalah = 2,3060

o

Daerah kritis penolakan : t

≤

2,3060

o

< -2,3060 dan t

o

3.

T hitung antara dua mean

> 2,3060

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Lanjutan Lampiran 21.

4.

Karena t

o

= 2.55 > 2.3060, maka hipotesa H

o

ditolak. Berarti terdapat

Lampiran 22. Pengujian Beda Nilai Rata-Rata Kadar HDL Setelah Pemberian

Lemak Sapi dan Minyak Kelapa Sawit Hasil Interesterifikasi

Hari ke-

Rata-rata Kadar (n=3) HDL (mg/dl)

Lemak Sapi

Minyak Kelapa Sawit

0

14.67

14.33

Kedua sampel adalah independen, maka dilakukan uji t beda antara dua mean dengan

taraf kepercayaan 95% untuk mengetahui apakah rata-rata kedua sampel

sama (σ

1

=

σ

2

)

atau berbeda (σ

1

≠ σ

2

)

2.

Nilai kritis t yang diperoleh dari tabel (t

2

0,05/2

Daerah kritis penerimaan : -2,3060

≤ t

(8))adalah = 2,3060

o

Daerah kritis penolakan : t

≤

2,3060

o

< -2,3060 dan t

o

3.

T hitung antara dua mean

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Lanjutan Lampiran 22.

4.

Karena t

o

= 3.05 > 2.3060, maka hipotesa H

o

ditolak. Berarti terdapat

Lampiran 23. Pengujian Beda Nilai Rata-Rata Kadar LDL Setelah Pemberian

Lemak Sapi dan Minyak Kelapa Sawit Hasil Interesterifikasi

Hari ke-

Rata-rata Kadar (n=3) LDL (mg/dl)

Lemak Sapi

Minyak Kelapa Sawit

0

20.27

20.07

Kedua sampel adalah independen, maka dilakukan uji t beda antara dua mean dengan

taraf kepercayaan 95% untuk mengetahui apakah rata-rata kedua sampel

sama (σ

1

=

σ

2

)

atau berbeda (σ

1

≠ σ

2

)

2.

Nilai kritis t yang diperoleh dari tabel (t

2

0,05/2

Daerah kritis penerimaan : -2,3060

≤ t

(8))adalah = 2,3060

o

Daerah kritis penolakan : t

≤

2,3060

o

< -2,3060 dan t

o

3.

T hitung antara dua mean

> 2,3060

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Lanjutan Lampiran 23.

4.

Karena t

o

= 3.07 > 2.3060, maka hipotesa H

o

ditolak. Berarti terdapat

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Lampiran 25. Gambar Alat

Microlab 300 (Vital Scientific)

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