Lampiran 1.Tanaman dan BuahAsam Bunga (Citrus cf. nobilis Lour)
1.1 Tanaman Asam Bunga (Citrus cf. nobilis Lour)
Lampiran 2. Hasil Uji Aktivitas Antioksidan Minyak Atsiri Kulit Buah Asam
Bunga
% Peredaman =
blanko
−
sampel
blanko
x
100%
1.
Konsentrasi 10 ppm
% Peredaman =
1,094
−
1,002
1,094
x
100%
= 8,40
%
2.
Konsentrasi 20 ppm
% Peredaman =
1,094
−
0,935
1,094
x
100%
= 14,53
%
3.
Konsentrasi 30 ppm
% Peredaman =
1,094
−
0
,883
1,094
x
100%
= 19,28
%
4.
Konsentrasi 40 ppm
% Peredaman =
1,094
−
0,832
1,094
x
100%
= 23,94
%
Peredaman radikal bebas oleh minyak atsiri kulit buah asam bunga
Sampel
Absorbansi
% Peredaman
Blanko
1,094
-
10 ppm
1,002
8,40
20 ppm
0,935
14,53
30 ppm
0,883
19,28
Lampiran 3. Perhitungan Nilai IC
50Minyak Atsiri Kulit Buah Asam Bunga
Jadi persamaan garis regresi Y = 0,5049X + 0,838
Lampiran 4. Hasil Data Kromatogram Hasil Analisa GC-MS Minyak Atsiri Kulit
Chromatogram minyak as bunga 1 C:\GCMSsolution\Data\Project1\analisa as bunga\minyak as bunga1.qgd
TIC*1.00
10.0 20.0 30.0 40.0
min
Peak Report TIC
Peak# R.Time Area Area% Height Name
1 6.706 1127495 0.64 407639 .ALPHA.-PINENE, (-)- $$ Bicyclo[3.1.1]hept-2-ene, 2,6,6-trim 2 8.166 1697541 0.96 596839 Sabinene $$ Bicyclo[3.1.0]hexane, 4-methylene-1-(1-methylethy 3 8.285 14096001 7.95 4632104 l-.beta.-Pinene $$ Bicyclo[3.1.1]heptane, 6,6-dimethyl-2-methyl 4 8.838 1711258 0.97 578553 .beta.-Myrcene $$ 1,6-Octadiene, 7-methyl-3-methylene- (CAS) 5 9.303 2147807 1.21 684545 Octanal (CAS) n-Octanal $$ n-Octylal $$ Antifoam-LF $$ Octa 6 10.161 6693231 3.77 1953476 Benzene, methyl(1-methylethyl)- (CAS) Cymol $$ Cymene $$ 7 10.371 79876032 45.05 17901825 l-Limonene $$ Cyclohexene, 1-methyl-4-(1-methylethenyl)-, (S) 8 10.440 6980228 3.94 1894115 1,8-Cineole $$ 2-Oxabicyclo[2.2.2]octane, 1,3,3-trimethyl- (CA 9 11.127 267467 0.15 91637 Trans-Ocimene $$ 1,3,7-Octatriene, 3,7-dimethyl- (CAS) 2,6-Di 10 11.551 13454664 7.59 4315105 .gamma.-Terpinene $$ 1,4-Cyclohexadiene, 1-methyl-4-(1-meth
11 12.048 1104647 0.62 343388 1-Nonanol
12 12.738 819417 0.46 262385 .ALPHA.-TERPINOLENE $$ Cyclohexene, 1-methyl-4-(1-met
13 13.197 2602799 1.47 829401 LINALOOL L $$
14 13.386 551567 0.31 168566 Nonanal (CAS) n-Nonanal $$ n-Nonylaldehyde $$ Nonaldehyde 15 15.323 2654401 1.50 843111 CITRONELLA $$ 6-Octenal, 3,7-dimethyl- (CAS) Citronellal $
16 15.727 319763 0.18 80673 MENTHOFURAN $$
17 15.829 427165 0.24 93260 Isoborneol
18 16.267 4379087 2.47 1331261 l-4-Terpineol $$ 3-Cyclohexen-1-ol, 4-methyl-1-(1-methylethyl) 19 16.793 9822624 5.54 2978368 .ALPHA. TERPINEOL $$
20 17.350 851112 0.48 241241 Decanal (CAS) n-Decanal $$ Decyl aldehyde $$ Decaldehyde $ 21 17.874 252622 0.14 74120 trans-Carveol $$ 2-Cyclohexen-1-ol, 2-methyl-5-(1-methylethen 22 18.216 4016044 2.26 1227934 .beta.-Citronellol $$ 6-Octen-1-ol, 3,7-dimethyl- (CAS) Citronel 23 18.697 2551487 1.44 801529 Z-Citral $$ 2,6-Octadienal, 3,7-dimethyl-, (Z)- (CAS) Neral $$ .
24 19.198 963006 0.54 295810 GERANIOL $$
25 19.796 3225504 1.82 882547 E-Citral $$ 2,6-Octadienal, 3,7-dimethyl-, (E)- (CAS) Geranial $ 26 21.458 305214 0.17 102271 Farnesol $$ 2,6,10-Dodecatrien-1-ol, 3,7,11-trimethyl- (CAS) F 27 22.262 624796 0.35 195853 .gamma.-Dodecalactone $$ 4-Hydroxydodecanoic acid lactone $ 28 22.412 3861812 2.18 1005849 Evodone $$
Lampiran 4. Kondisi Alat GC-MS
Method [Comment]
===== Analytical Line 1 =====
[AOC-20i]
# of Rinses with Presolvent 2
# of Rinses with Solvent(post) 3
# of Rinses with Sample 2
Plunger Speed(Suction) :High
Viscosity Comp. Time :0.2 sec
Plunger Speed(Injection) :Middle
Syringe Insertion Speed :High
Injection Mode :Normal
Pumping Times 5
Inj. Port Dwell Time :0.3 sec
Terminal Air Gap :No
Plunger Washing Speed :High
Washing Volume :8uL
Syringe Suction Position :0.0 mm
Syringe Injection Position :0.0 mm
Use 3 Solvent Vial :1 vial
[GC-2010]
Column Oven Temp. :60.0 °C
Injection Temp. :270.00 °C
Injection Mode :Split
Flow Control Mode :Pressure
Pressure :70.0 kPa
Total Flow :62.4 mL/min
Column Flow :1.16 mL/min
Linear Velocity :39.4 cm/sec
Purge Flow :3.0 mL/min
Split Ratio :50.0
High Pressure Injection :OFF
Carrier Gas Saver :OFF
Splitter Hold :OFF
Oven Temp. Program
Rate Temperature(°C) Hold Time(min)
- 60.0 5.00
4.00 260.0 10.00
2.00 280.0 5.00
< Ready Check Heat Unit >
Column Oven : Yes
SPL1 : Yes
MS : Yes
< Ready Check Detector(FTD) > < Ready Check Baseline Drift > < Ready Check Injection Flow >
SPL1 Carrier : Yes
SPL1 Purge : Yes
< Ready Check APC Flow > < Ready Check Detector APC Flow >
External Wait :No
Equilibrium Time :3.0 min
[GC Program]
[GCMS-QP2010 Plus]
IonSourceTemp :280.00 °C
Interface Temp. :270.00 °C
Solvent Cut Time :0.00 min
Detector Gain Mode :Relative Detector Gain :0.00 kV
Threshold 1000
[MS Table] --Group 1 - Event 1--
Start Time :1.00min
End Time :80.00min
ACQ Mode :Scan
Event Time :0.50sec
