B1J010045 14.

(1)

Lampiran 1. Nama, Spesifikasi, dan Kegunaan Bahan Penelitian

No. Nama Bahan Spesifikasi Kegunaan

1. Larva ikan nilem Berumur 1 hari Hewan uji 2. Pakan ikan

komersial

41% protein, 6% lemak, serat 2,5%, abu 16%, air 11%

Pakan larva 3. K2Cr2O7 Diencerkan menjadi konsentrasi 5

ppm, 10 ppm, dan 15 ppm

Sumber Cr (VI) dan sebagai media pemeliharaan

(2)

30 Lampiran 2. Nama, Tipe, Kegunaan, dan Keberadaan Peralatan yang

Digunakan Selama Penelitian

No. Nama Alat Tipe Kegunaan Tempat

1. Aerator Recent Sumber oksigen Lab. Struktur Perkembangan Hewan

2. Eye piece micrometer

Mengukur sirip post-larva

Lab. Struktur Perkembangan Hewan

3. Gelas ukur 2000 ml - Mengukur volume air Lab. Struktur Perkembangan Hewan

4. Mikroskop cahaya Olympus Mengamati sirip post-larva

5. Milimeter blok - Mengukur panjang tubuh post-larva

6. Saringan - Menyaring oosit dari milt

7. Sarung tangan Melindungi tangan dari media krom

8. Sendok plastik - Mengambil post-larva Lab. Struktur Perkembangan Hewan

9. Spuit injeksi 1 ml Mengambil milt Lab. Struktur Perkembangan Hewan

10. Piring kecil - Tempat fertilisasi oosit Lab. Struktur Perkembangan Hewan

11. Timbangan analitik OHas Menimbang berat K2Cr207

Lab. Lingkungan 12. Wadah baskom - Tempat pemeliharaan

larva

(3)

Lampiran 3. Skema Bagan Metode Kerja

Mg-1 Mg-2 Mg-3 Mg-4 Mg-5 Mg-6 Mg-7 Mg-8

Sampel :

o

Diukur panjang tubuh

o

Dievaluasi bentuk dan ukuran sirip (dorsal, pectoral, abdominal, anal, dan

caudal)

o

Dievaluasi morfologi insang

TABULASI DATA

Perlakuan Ulangan Waktu Pengambilan Data

Mg-1 Mg-2 Mg-3 Mg-4 Mg-5 Mg-6 Mg-7 Mg-8 0 ppm 1 PENGADAAN LARVA UJI

PENGADAAN LARVA UJI

(4)

32 TABULASI DATA (Lanjutan).

Perlakuan Ulangan Waktu Pengambilan Data

Mg-1 Mg-2 Mg-3 Mg-4 Mg-5 Mg-6 Mg-7 Mg-8 10 ppm 1

2 3 4 5 6 15 ppm 1 2 3 4 5 6

Analisis Data:

o

Morfologi sirip dan insang

→ deskriptif

o

Panjang tubuh

→ Anova 1 arah pada tingkat signifikansi p<0,05

(5)

Lampiran 4. Data Hasil Uji LC

50

No. Konsentrasi Cr

(ppm)

Gambar 4.1. Grafik

presentase larva yang hidup pada masing-masing

konsentrasi yang diujikan

0

LC 50 KROM HEKSAVALEN

(6)

34 Lampiran 5. Hasil Analisis ANOVA Luas Kantong Yolk Post-larva Ikan Nilem

(

O. hasselti

C. V.) Hari Ke 2-4

Tests of Between-Subjects Effects

Dependent Variable:luas kantong yolk

Source

Type III Sum

of Squares df Mean Square F Sig. Corrected Model 8.900E11a ₁₁ _8.091E10 _12.132 _.000

Intercept 4.419E12 1 4.419E12 662.675 .000 perlakuan 9.685E10 3 3.228E10 4.841 .005 hari 7.164E11 2 3.582E11 53.710 .000 perlakuan * hari 7.676E10 6 1.279E10 1.918 .097 Error 3.201E11 48 6.669E9

Total 5.629E12 60 Corrected Total 1.210E12 59

a. R Squared = .735 (Adjusted R Squared = .675)

hari pengamatan

Multiple Comparisons

luas kantong yolk

LSD

(I) hari penga matan

(J) hari penga matan

Mean

Difference (I-J) Std. Error Sig.

95% Confidence Interval Lower Bound Upper Bound 2 3 193168.3022* _2.58241E4 _.000 _141245.5291 _245091.0754

4 257025.5922* _2.58241E4 _.000 _205102.8191 _308948.3654

3 2 -1.9317E5* _2.58241E4 _.000 _-2.4509E5 _-1.4125E5

4 63857.2900* _2.58241E4 _.017 _11934.5169 _115780.0631

4 2 -2.5703E5* _2.58241E4 _.000 _-3.0895E5 _-2.0510E5

3 -63857.2900* _2.58241E4 _.017 _-1.1578E5 _-11934.5169

Based on observed means.

The error term is Mean Square(Error) = 6668826013.799. *. The mean difference is significant at the .05 level.

(7)

perlakuan penelitian

Multiple Comparisons

luas kantong yolk

LSD

(I) perlakua n penelitia n

(J) perlakua n penelitia n

Mean

95% Confidence Interval

Lower Bound Upper Bound kontrol 5 ppm 25542.9160 2.98191E4 .396 -34412.3381 85498.1701

10 ppm -14900.0343 2.98191E4 .620 -74855.2884 45055.2197 15 ppm -83014.4770* _2.98191E4 _.008 _-1.4297E5 _-23059.2229

5 ppm kontrol -25542.9160 2.98191E4 .396 -85498.1701 34412.3381 10 ppm -40442.9503 2.98191E4 .181 -1.0040E5 19512.3037 15 ppm -1.0856E5* _2.98191E4 _.001 _-1.6851E5 _-48602.1389

10 ppm kontrol 14900.0343 2.98191E4 .620 -45055.2197 74855.2884 5 ppm 40442.9503 2.98191E4 .181 -19512.3037 100398.2044 15 ppm -68114.4427* _2.98191E4 _.027 _-1.2807E5 _-8159.1886

15 ppm kontrol 83014.4770* _2.98191E4 _.008 _23059.2229 _142969.7311

5 ppm 108557.3930* _2.98191E4 _.001 _48602.1389 _168512.6471

10 ppm 68114.4427* _2.98191E4 _.027 _8159.1886 _128069.6967

(8)

36 Lampiran 6. Hasil Analisis ANOVA Tinggi membran sirip punggung (

dorsal

fin

) Post-larva Ikan Nilem (

O. hasselti

C. V.) Hari Ke 2-7

Tests of Between-Subjects Effects

Dependent Variable:tinggi membran dorsal

Source

Type III Sum

of Squares df Mean Square F Sig. Corrected Model 152491.209a ₂₃ _6630.053 _7.644 _.000

Intercept 4083545.981 1 4083545.981 4.708E3 .000 perlakuan 50106.687 3 16702.229 19.256 .000 hari 50255.687 5 10051.137 11.588 .000 perlakuan * hari 52128.834 15 3475.256 4.007 .000 Error 83269.906 96 867.395

Total 4319307.096 120 Corrected Total 235761.115 119

hari pengamatan

Multiple Comparisons

tinggi membran dorsal

LSD

95% Confidence Interval Lower Bound Upper Bound 2 3 -16.4255 9.31340 .081 -34.9125 2.0615

4 -30.3240* _9.31340 _.002 _-48.8110 _-11.8370

5 -41.6955* _9.31340 _.000 _-60.1825 _-23.2085

6 -45.4860* _9.31340 _.000 _-63.9730 _-26.9990

7 -63.1750* _9.31340 _.000 _-81.6620 _-44.6880

3 2 16.4255 9.31340 .081 -2.0615 34.9125 4 -13.8985 9.31340 .139 -32.3855 4.5885 5 -25.2700* _9.31340 _.008 _-43.7570 _-6.7830

6 -29.0605* _9.31340 _.002 _-47.5475 _-10.5735

7 -46.7495* _9.31340 _.000 _-65.2365 _-28.2625

4 2 30.3240* _9.31340 _.002 _11.8370 _48.8110

3 13.8985 9.31340 .139 -4.5885 32.3855 5 -11.3715 9.31340 .225 -29.8585 7.1155 6 -15.1620 9.31340 .107 -33.6490 3.3250 7 -32.8510* _9.31340 _.001 _-51.3380 _-14.3640

5 2 41.6955* _9.31340 _.000 _23.2085 _60.1825

3 25.2700* _9.31340 _.008 _6.7830 _43.7570

4 11.3715 9.31340 .225 -7.1155 29.8585

(9)

6 -3.7905 9.31340 .685 -22.2775 14.6965 7 -21.4795* _9.31340 _.023 _-39.9665 _-2.9925

6 2 45.4860* _9.31340 _.000 _26.9990 _63.9730

3 29.0605* _9.31340 _.002 _10.5735 _47.5475

4 15.1620 9.31340 .107 -3.3250 33.6490 5 3.7905 9.31340 .685 -14.6965 22.2775 7 -17.6890 9.31340 .061 -36.1760 .7980 7 2 63.1750* _9.31340 _.000 _44.6880 _81.6620

3 46.7495* _9.31340 _.000 _28.2625 _65.2365

4 32.8510* _9.31340 _.001 _14.3640 _51.3380

5 21.4795* _9.31340 _.023 _2.9925 _39.9665

6 17.6890 9.31340 .061 -.7980 36.1760

perlakuan penelitian

Multiple Comparisons

tinggi membran dorsal

LSD

(I-J) Std. Error Sig.

95% Confidence Interval Lower Bound Upper Bound kontrol 5 ppm 39.5897* _7.60436 _.000 _24.4951 _54.6842

10 ppm 46.3283* _7.60436 _.000 _31.2338 _61.4229

15 ppm 52.2247* _7.60436 _.000 _37.1301 _67.3192

5 ppm kontrol -39.5897* _7.60436 _.000 _-54.6842 _-24.4951

10 ppm 6.7387 7.60436 .378 -8.3559 21.8332 15 ppm 12.6350 7.60436 .100 -2.4595 27.7295 10 ppm kontrol -46.3283* _7.60436 _.000 _-61.4229 _-31.2338

5 ppm -6.7387 7.60436 .378 -21.8332 8.3559 15 ppm 5.8963 7.60436 .440 -9.1982 20.9909 15 ppm kontrol -52.2247* _7.60436 _.000 _-67.3192 _-37.1301

5 ppm -12.6350 7.60436 .100 -27.7295 2.4595 10 ppm -5.8963 7.60436 .440 -20.9909 9.1982

(10)

38 Lampiran 7. Hasil Analisis ANOVA Tinggi membran membran sirip perut

(

abdominal fin

) Post-larva Ikan Nilem (

O. hasselti

C. V.) Hari Ke

2-7

Tests of Between-Subjects Effects

Dependent Variable:tinggi membran abdominal

Source

Type III Sum

of Squares df Mean Square F Sig. Corrected Model 356749.393a ₂₃ _15510.843 _9.684 _.000

Intercept 4046338.467 1 4046338.467 2.526E3 .000 perlakuan 134313.167 3 44771.056 27.951 .000 hari 77054.463 5 15410.893 9.621 .000 perlakuan * hari 145381.764 15 9692.118 6.051 .000 Error 153768.354 96 1601.754

Total 4556856.214 120 Corrected Total 510517.748 119

hari pengamatan

Multiple Comparisons

tinggi membran abdominal

LSD

95% Confidence Interval Lower Bound Upper Bound 2 3 -16.4255 12.65604 .197 -41.5475 8.6965

4 -34.1145* _12.65604 _.008 _-59.2365 _-8.9925

5 -36.6415* _12.65604 _.005 _-61.7635 _-11.5195

6 -58.1210* _12.65604 _.000 _-83.2430 _-32.9990

7 -77.0735* _12.65604 _.000 _-102.1955 _-51.9515

3 2 16.4255 12.65604 .197 -8.6965 41.5475 4 -17.6890 12.65604 .165 -42.8110 7.4330 5 -20.2160 12.65604 .113 -45.3380 4.9060 6 -41.6955* _12.65604 _.001 _-66.8175 _-16.5735

7 -60.6480* _12.65604 _.000 _-85.7700 _-35.5260

4 2 34.1145* _12.65604 _.008 _8.9925 _59.2365

3 17.6890 12.65604 .165 -7.4330 42.8110 5 -2.5270 12.65604 .842 -27.6490 22.5950 6 -24.0065 12.65604 .061 -49.1285 1.1155 7 -42.9590* _12.65604 _.001 _-68.0810 _-17.8370

5 2 36.6415* _12.65604 _.005 _11.5195 _61.7635

3 20.2160 12.65604 .113 -4.9060 45.3380 4 2.5270 12.65604 .842 -22.5950 27.6490

(11)

6 -21.4795 12.65604 .093 -46.6015 3.6425 7 -40.4320* _12.65604 _.002 _-65.5540 _-15.3100

6 2 58.1210* _12.65604 _.000 _32.9990 _83.2430

3 41.6955* _12.65604 _.001 _16.5735 _66.8175

4 24.0065 12.65604 .061 -1.1155 49.1285 5 21.4795 12.65604 .093 -3.6425 46.6015 7 -18.9525 12.65604 .138 -44.0745 6.1695 7 2 77.0735* _12.65604 _.000 _51.9515 _102.1955

3 60.6480* _12.65604 _.000 _35.5260 _85.7700

4 42.9590* _12.65604 _.001 _17.8370 _68.0810

5 40.4320* _12.65604 _.002 _15.3100 _65.5540

6 18.9525 12.65604 .138 -6.1695 44.0745

perlakuan penelitian

Multiple Comparisons

tinggi membran abdominal

LSD

(I-J) Std. Error Sig.

95% Confidence Interval Lower Bound Upper Bound kontrol 5 ppm 69.9137* _10.33361 _.000 _49.4016 _90.4257

10 ppm 55.5940* _10.33361 _.000 _35.0819 _76.1061

15 ppm 90.1297* _10.33361 _.000 _69.6176 _110.6417

5 ppm kontrol -69.9137* _10.33361 _.000 _-90.4257 _-49.4016

10 ppm -14.3197 10.33361 .169 -34.8317 6.1924 15 ppm 20.2160 10.33361 .053 -.2961 40.7281 10 ppm kontrol -55.5940* _10.33361 _.000 _-76.1061 _-35.0819

5 ppm 14.3197 10.33361 .169 -6.1924 34.8317 15 ppm 34.5357* _10.33361 _.001 _14.0236 _55.0477

15 ppm kontrol -90.1297* _10.33361 _.000 _-110.6417 _-69.6176

5 ppm -20.2160 10.33361 .053 -40.7281 .2961 10 ppm -34.5357* _10.33361 _.001 _-55.0477 _-14.0236

(12)

40 Lampiran 8. Hasil Analisis ANOVA Tinggi Membran Sirip Ekor (

caudal fin

)

Post-larva Ikan Nilem (

O. hasselti

C. V.) Hari Ke 2-7

Tests of Between-Subjects Effects

Dependent Variable:tinggi membran caudal

Source

Type III Sum

of Squares df Mean Square F Sig. Corrected Model 1.628E6a ₂₃ _70772.387 _14.851 _.000

Intercept 7.396E7 1 7.396E7 1.552E4 .000 perlakuan 569223.883 3 189741.294 39.817 .000 hari 469649.082 5 93929.816 19.711 .000 perlakuan * hari 588891.928 15 39259.462 8.239 .000 Error 457473.626 96 4765.350

Total 7.604E7 120 Corrected Total 2085238.519 119

hari pengamatan

Multiple Comparisons

tinggi membran caudal

LSD

95% Confidence Interval Lower Bound Upper Bound 2 3 -75.8100* _21.82968 _.001 _-119.1416 _-32.4784

4 -137.7215* _21.82968 _.000 _-181.0531 _-94.3899

5 -131.4040* _21.82968 _.000 _-174.7356 _-88.0724

6 -169.3090* _21.82968 _.000 _-212.6406 _-125.9774

7 -185.7345* _21.82968 _.000 _-229.0661 _-142.4029

3 2 75.8100* _21.82968 _.001 _32.4784 _119.1416

4 -61.9115* _21.82968 _.006 _-105.2431 _-18.5799

5 -55.5940* _21.82968 _.012 _-98.9256 _-12.2624

6 -93.4990* _21.82968 _.000 _-136.8306 _-50.1674

7 -109.9245* _21.82968 _.000 _-153.2561 _-66.5929

4 2 137.7215* _21.82968 _.000 _94.3899 _181.0531

3 61.9115* _21.82968 _.006 _18.5799 _105.2431

5 6.3175 21.82968 .773 -37.0141 49.6491 6 -31.5875 21.82968 .151 -74.9191 11.7441 7 -48.0130* _21.82968 _.030 _-91.3446 _-4.6814

5 2 131.4040* _21.82968 _.000 _88.0724 _174.7356

3 55.5940* _21.82968 _.012 _12.2624 _98.9256

4 -6.3175 21.82968 .773 -49.6491 37.0141

(13)

6 -37.9050 21.82968 .086 -81.2366 5.4266 7 -54.3305* _21.82968 _.015 _-97.6621 _-10.9989

6 2 169.3090* _21.82968 _.000 _125.9774 _212.6406

3 93.4990* _21.82968 _.000 _50.1674 _136.8306

4 31.5875 21.82968 .151 -11.7441 74.9191 5 37.9050 21.82968 .086 -5.4266 81.2366 7 -16.4255 21.82968 .454 -59.7571 26.9061 7 2 185.7345* _21.82968 _.000 _142.4029 _229.0661

3 109.9245* _21.82968 _.000 _66.5929 _153.2561

4 48.0130* _21.82968 _.030 _4.6814 _91.3446

5 54.3305* _21.82968 _.015 _10.9989 _97.6621

6 16.4255 21.82968 .454 -26.9061 59.7571

perlakuan peneltian

Multiple Comparisons

tinggi membran caudal

LSD

95% Confidence Interval

Lower Bound Upper Bound kontrrol 5 ppm 134.7733* _17.82386 _.000 _99.3932 _170.1534

10 ppm 164.2550* _17.82386 _.000 _128.8749 _199.6351

15 ppm 169.3090* _17.82386 _.000 _133.9289 _204.6891

5 ppm kontrrol -134.7733* _17.82386 _.000 _-170.1534 _-99.3932

10 ppm 29.4817 17.82386 .101 -5.8984 64.8618 15 ppm 34.5357 17.82386 .056 -.8444 69.9158 10 ppm kontrrol -164.2550* _17.82386 _.000 _-199.6351 _-128.8749

5 ppm -29.4817 17.82386 .101 -64.8618 5.8984 15 ppm 5.0540 17.82386 .777 -30.3261 40.4341 15 ppm kontrrol -169.3090* _17.82386 _.000 _-204.6891 _-133.9289

5 ppm -34.5357 17.82386 .056 -69.9158 .8444 10 ppm -5.0540 17.82386 .777 -40.4341 30.3261

(14)

42 Lampiran 9. Hasil Analisis Uji t Tinggi membran membran sirip perut

(

abdominal fin

) Post-larva Ikan Nilem (

O. hasselti

C. V.)

Perlakuan Kontrol dengan 5 ppm Minggu Ke 2

Group Statistics

perlakuan

penelitian N Mean Std. Deviation Std. Error Mean tinggi membran abdominal kontrol 5 16.3902 2.44393 1.09296

5 ppm 5 5.1038 6.12838 2.74070

Independent Samples Test

Levene's Test for Equality

of Variances t-test for Equality of Means

F Sig. t df

Sig. (2-tailed)

Mean Difference

Std. Error Difference

95% Confidence Interval of the

Difference Lower Upper tinggi

membran abdominal

Equal varianc es assume d

12.231 .008 3.825 8 .005 11.28641 2.95059 4.48234 18.09048

Equal varianc es not assume d

3.825 5.241 .011 11.28641 2.95059 3.80535 18.76748

(15)

Lampiran 10. Hasil Analisis ANOVA Panjang Sirip Punggung (

dorsal fin

)

Post-larva Ikan Nilem (

O. hasselti

C. V.) (µm) Perlakuan

Kontrol Umur 2-8 minggu

ANOVA

panjang sirip dorsal

Sum of Squares df Mean Square F Sig. Between Groups 1.687E8 6 2.811E7 71.738 .000 Within Groups 1.097E7 28 391864.821

Total 1.796E8 34

Post Hoc Tests

Multiple Comparisons

panjang sirip dorsal

LSD

95% Confidence Interval Lower Bound Upper Bound 14 21 -1283.71600* _3.95912E2 _.003 _-2094.7040 _-472.7280

28 -2365.27200* _3.95912E2 _.000 _-3176.2600 _-1554.2840

35 -3790.50000* _3.95912E2 _.000 _-4601.4880 _-2979.5120

42 -4331.27800* _3.95912E2 _.000 _-5142.2660 _-3520.2900

49 -5711.02000* _3.95912E2 _.000 _-6522.0080 _-4900.0320

56 -6605.57800* _3.95912E2 _.000 _-7416.5660 _-5794.5900

21 14 1283.71600* _3.95912E2 _.003 _472.7280 _2094.7040

28 -1081.55600* _3.95912E2 _.011 _-1892.5440 _-270.5680

35 -2506.78400* _3.95912E2 _.000 _-3317.7720 _-1695.7960

42 -3047.56200* _3.95912E2 _.000 _-3858.5500 _-2236.5740

49 -4427.30400* _3.95912E2 _.000 _-5238.2920 _-3616.3160

56 -5321.86200* _3.95912E2 _.000 _-6132.8500 _-4510.8740

28 14 2365.27200* _3.95912E2 _.000 _1554.2840 _3176.2600

21 1081.55600* _3.95912E2 _.011 _270.5680 _1892.5440

35 -1425.22800* _3.95912E2 _.001 _-2236.2160 _-614.2400

42 -1966.00600* _3.95912E2 _.000 _-2776.9940 _-1155.0180

49 -3345.74800* _3.95912E2 _.000 _-4156.7360 _-2534.7600

56 -4240.30600* _3.95912E2 _.000 _-5051.2940 _-3429.3180

35 14 3790.50000* _3.95912E2 _.000 _2979.5120 _4601.4880

21 2506.78400* _3.95912E2 _.000 _1695.7960 _3317.7720

28 1425.22800* _3.95912E2 _.001 _614.2400 _2236.2160

42 -540.77800 3.95912E2 .183 -1351.7660 270.2100 49 -1920.52000* _3.95912E2 _.000 _-2731.5080 _-1109.5320

56 -2815.07800* _3.95912E2 _.000 _-3626.0660 _-2004.0900

(16)

44

42 14 4331.27800* _3.95912E2 _.000 _3520.2900 _5142.2660

21 3047.56200* _3.95912E2 _.000 _2236.5740 _3858.5500

28 1966.00600* _3.95912E2 _.000 _1155.0180 _2776.9940

35 540.77800 3.95912E2 .183 -270.2100 1351.7660 49 -1379.74200* _3.95912E2 _.002 _-2190.7300 _-568.7540

56 -2274.30000* _3.95912E2 _.000 _-3085.2880 _-1463.3120

49 14 5711.02000* _3.95912E2 _.000 _4900.0320 _6522.0080

21 4427.30400* _3.95912E2 _.000 _3616.3160 _5238.2920

28 3345.74800* _3.95912E2 _.000 _2534.7600 _4156.7360

35 1920.52000* _3.95912E2 _.000 _1109.5320 _2731.5080

42 1379.74200* _3.95912E2 _.002 _568.7540 _2190.7300

56 -894.55800* _3.95912E2 _.032 _-1705.5460 _-83.5700

56 14 6605.57800* _3.95912E2 _.000 _5794.5900 _7416.5660

21 5321.86200* _3.95912E2 _.000 _4510.8740 _6132.8500

28 4240.30600* _3.95912E2 _.000 _3429.3180 _5051.2940

35 2815.07800* _3.95912E2 _.000 _2004.0900 _3626.0660

42 2274.30000* _3.95912E2 _.000 _1463.3120 _3085.2880

49 894.55800* _3.95912E2 _.032 _83.5700 _1705.5460

*. The mean difference is significant at the 0.05 level.

(17)

Lampiran 11. Hasil Analisis ANOVA Panjang Sirip Perut (

abdominal fin

)

Post-larva Ikan Nilem (

O. hasselti

C. V.) (µm) Perlakuan Kontrol

Umur 3-8 minggu

ANOVA

panjang sirip abdominal

Sum of Squares df Mean Square F Sig. Between Groups 2.097E7 5 4194117.007 34.288 .000 Within Groups 2935647.336 24 122318.639

Total 2.391E7 29

Post Hoc Tests

Multiple Comparisons

panjang sirip abdominal

LSD

35 -1238.23000* _2.21196E2 _.000 _-1694.7551 _-781.7049

42 -1571.79400* _2.21196E2 _.000 _-2028.3191 _-1115.2689

49 -1753.73800* _2.21196E2 _.000 _-2210.2631 _-1297.2129

56 -2668.51200* _2.21196E2 _.000 _-3125.0371 _-2211.9869

28 21 707.56000* _2.21196E2 _.004 _251.0349 _1164.0851

35 -530.67000* _2.21196E2 _.025 _-987.1951 _-74.1449

42 -864.23400* _2.21196E2 _.001 _-1320.7591 _-407.7089

49 -1046.17800* _2.21196E2 _.000 _-1502.7031 _-589.6529

56 -1960.95200* _2.21196E2 _.000 _-2417.4771 _-1504.4269

35 21 1238.23000* _2.21196E2 _.000 _781.7049 _1694.7551

28 530.67000* _2.21196E2 _.025 _74.1449 _987.1951

42 -333.56400 2.21196E2 .145 -790.0891 122.9611 49 -515.50800* _2.21196E2 _.029 _-972.0331 _-58.9829

56 -1430.28200* _2.21196E2 _.000 _-1886.8071 _-973.7569

42 21 1571.79400* _2.21196E2 _.000 _1115.2689 _2028.3191

28 864.23400* _2.21196E2 _.001 _407.7089 _1320.7591

35 333.56400 2.21196E2 .145 -122.9611 790.0891 49 -181.94400 2.21196E2 .419 -638.4691 274.5811 56 -1096.71800* _2.21196E2 _.000 _-1553.2431 _-640.1929

49 21 1753.73800* _2.21196E2 _.000 _1297.2129 _2210.2631

28 1046.17800* _2.21196E2 _.000 _589.6529 _1502.7031

35 515.50800* _2.21196E2 _.029 _58.9829 _972.0331

42 181.94400 2.21196E2 .419 -274.5811 638.4691

(18)

46

56 -914.77400* _2.21196E2 _.000 _-1371.2991 _-458.2489

56 21 2668.51200* _2.21196E2 _.000 _2211.9869 _3125.0371

28 1960.95200* _2.21196E2 _.000 _1504.4269 _2417.4771

35 1430.28200* _2.21196E2 _.000 _973.7569 _1886.8071

42 1096.71800* _2.21196E2 _.000 _640.1929 _1553.2431

49 914.77400* _2.21196E2 _.000 _458.2489 _1371.2991

(19)

Lampiran 12. Hasil Analisis ANOVA Panjang Sirip Dubur (

anal fin

)

Post-larva Ikan Nilem (

O. hasselti

C. V.) (µm) Perlakuan Kontrol

Umur 2-8 minggu

ANOVA

panjang sirip anal

Total 4.930E7 34

Post Hoc Tests

Multiple Comparisons

panjang sirip anal

LSD

28 -1445.44400* _2.12869E2 _.000 _-1881.4860 _-1009.4020

35 -2026.65400* _2.12869E2 _.000 _-2462.6960 _-1590.6120

42 -2425.92000* _2.12869E2 _.000 _-2861.9620 _-1989.8780

49 -3113.26400* _2.12869E2 _.000 _-3549.3060 _-2677.2220

56 -3487.26000* _2.12869E2 _.000 _-3923.3020 _-3051.2180

21 14 838.96400* _2.12869E2 _.000 _402.9220 _1275.0060

28 -606.48000* _2.12869E2 _.008 _-1042.5220 _-170.4380

35 -1187.69000* _2.12869E2 _.000 _-1623.7320 _-751.6480

42 -1586.95600* _2.12869E2 _.000 _-2022.9980 _-1150.9140

49 -2274.30000* _2.12869E2 _.000 _-2710.3420 _-1838.2580

56 -2648.29600* _2.12869E2 _.000 _-3084.3380 _-2212.2540

28 14 1445.44400* _2.12869E2 _.000 _1009.4020 _1881.4860

21 606.48000* _2.12869E2 _.008 _170.4380 _1042.5220

35 -581.21000* _2.12869E2 _.011 _-1017.2520 _-145.1680

42 -980.47600* _2.12869E2 _.000 _-1416.5180 _-544.4340

49 -1667.82000* _2.12869E2 _.000 _-2103.8620 _-1231.7780

56 -2041.81600* _2.12869E2 _.000 _-2477.8580 _-1605.7740

35 14 2026.65400* _2.12869E2 _.000 _1590.6120 _2462.6960

21 1187.69000* _2.12869E2 _.000 _751.6480 _1623.7320

28 581.21000* _2.12869E2 _.011 _145.1680 _1017.2520

42 -399.26600 2.12869E2 .071 -835.3080 36.7760 49 -1086.61000* _2.12869E2 _.000 _-1522.6520 _-650.5680

56 -1460.60600* _2.12869E2 _.000 _-1896.6480 _-1024.5640

(20)

48

42 14 2425.92000* _2.12869E2 _.000 _1989.8780 _2861.9620

21 1586.95600* _2.12869E2 _.000 _1150.9140 _2022.9980

28 980.47600* _2.12869E2 _.000 _544.4340 _1416.5180

35 399.26600 2.12869E2 .071 -36.7760 835.3080 49 -687.34400* _2.12869E2 _.003 _-1123.3860 _-251.3020

56 -1061.34000* _2.12869E2 _.000 _-1497.3820 _-625.2980

49 14 3113.26400* _2.12869E2 _.000 _2677.2220 _3549.3060

21 2274.30000* _2.12869E2 _.000 _1838.2580 _2710.3420

28 1667.82000* _2.12869E2 _.000 _1231.7780 _2103.8620

35 1086.61000* _2.12869E2 _.000 _650.5680 _1522.6520

42 687.34400* _2.12869E2 _.003 _251.3020 _1123.3860

56 -373.99600 2.12869E2 .090 -810.0380 62.0460 56 14 3487.26000* _2.12869E2 _.000 _3051.2180 _3923.3020

21 2648.29600* _2.12869E2 _.000 _2212.2540 _3084.3380

28 2041.81600* _2.12869E2 _.000 _1605.7740 _2477.8580

35 1460.60600* _2.12869E2 _.000 _1024.5640 _1896.6480

42 1061.34000* _2.12869E2 _.000 _625.2980 _1497.3820

49 373.99600 2.12869E2 .090 -62.0460 810.0380

(21)

Lampiran 13. Hasil Analisis ANOVA Panjang Sirip Ekor (

caudal fin

) Post-larva

Ikan Nilem (

O. hasselti

C. V.) (µm) Perlakuan Kontrol Umur 2-8

minggu

ANOVA

panjang sirip caudal

Sum of Squares df Mean Square F Sig. Between Groups 7.263E7 6 1.211E7 59.598 .000 Within Groups 5687385.677 28 203120.917

Total 7.832E7 34

Post Hoc Tests

Multiple Comparisons

panjang sirip caudal

LSD

28 -2067.08600* _2.85041E2 _.000 _-2650.9660 _-1483.2060

35 -2537.10800* _2.85041E2 _.000 _-3120.9880 _-1953.2280

42 -3072.83200* _2.85041E2 _.000 _-3656.7120 _-2488.9520

49 -3851.14800* _2.85041E2 _.000 _-4435.0280 _-3267.2680

56 -4472.79000* _2.85041E2 _.000 _-5056.6700 _-3888.9100

21 14 1061.34000* _2.85041E2 _.001 _477.4600 _1645.2200

28 -1005.74600* _2.85041E2 _.001 _-1589.6260 _-421.8660

35 -1475.76800* _2.85041E2 _.000 _-2059.6480 _-891.8880

42 -2011.49200* _2.85041E2 _.000 _-2595.3720 _-1427.6120

49 -2789.80800* _2.85041E2 _.000 _-3373.6880 _-2205.9280

56 -3411.45000* _2.85041E2 _.000 _-3995.3300 _-2827.5700

28 14 2067.08600* _2.85041E2 _.000 _1483.2060 _2650.9660

21 1005.74600* _2.85041E2 _.001 _421.8660 _1589.6260

35 -470.02200 2.85041E2 .110 -1053.9020 113.8580 42 -1005.74600* _2.85041E2 _.001 _-1589.6260 _-421.8660

49 -1784.06200* _2.85041E2 _.000 _-2367.9420 _-1200.1820

56 -2405.70400* _2.85041E2 _.000 _-2989.5840 _-1821.8240

35 14 2537.10800* _2.85041E2 _.000 _1953.2280 _3120.9880

21 1475.76800* _2.85041E2 _.000 _891.8880 _2059.6480

28 470.02200 2.85041E2 .110 -113.8580 1053.9020 42 -535.72400 2.85041E2 .071 -1119.6040 48.1560 49 -1314.04000* _2.85041E2 _.000 _-1897.9200 _-730.1600

56 -1935.68200* _2.85041E2 _.000 _-2519.5620 _-1351.8020

(22)

50

42 14 3072.83200* _2.85041E2 _.000 _2488.9520 _3656.7120

21 2011.49200* _2.85041E2 _.000 _1427.6120 _2595.3720

28 1005.74600* _2.85041E2 _.001 _421.8660 _1589.6260

35 535.72400 2.85041E2 .071 -48.1560 1119.6040 49 -778.31600* _2.85041E2 _.011 _-1362.1960 _-194.4360

56 -1399.95800* _2.85041E2 _.000 _-1983.8380 _-816.0780

49 14 3851.14800* _2.85041E2 _.000 _3267.2680 _4435.0280

21 2789.80800* _2.85041E2 _.000 _2205.9280 _3373.6880

28 1784.06200* _2.85041E2 _.000 _1200.1820 _2367.9420

35 1314.04000* _2.85041E2 _.000 _730.1600 _1897.9200

42 778.31600* _2.85041E2 _.011 _194.4360 _1362.1960

56 -621.64200* _2.85041E2 _.038 _-1205.5220 _-37.7620

56 14 4472.79000* _2.85041E2 _.000 _3888.9100 _5056.6700

21 3411.45000* _2.85041E2 _.000 _2827.5700 _3995.3300

28 2405.70400* _2.85041E2 _.000 _1821.8240 _2989.5840

35 1935.68200* _2.85041E2 _.000 _1351.8020 _2519.5620

42 1399.95800* _2.85041E2 _.000 _816.0780 _1983.8380

49 621.64200* _2.85041E2 _.038 _37.7620 _1205.5220

(23)

Lampiran 14. Hasil Analisis ANOVA Lebar Sirip Punggung (

dorsal fin

)

Post-larva Ikan Nilem (

O. hasselti

C. V.) (µm) Perlakuan Kontrol

Umur 2-8 minggu

ANOVA

lebar sirip dorsal

Sum of Squares df Mean Square F Sig. Between Groups 2.397E7 6 3994547.164 7.583 .000 Within Groups 1.475E7 28 526786.153

Total 3.872E7 34

Post Hoc Tests

Multiple Comparisons

lebar sirip dorsal

LSD

95% Confidence Interval Lower Bound Upper Bound 14 21 -328.51000 4.59036E2 .480 -1268.8035 611.7835

28 -389.15800 4.59036E2 .404 -1329.4515 551.1355 35 -1334.25600* _4.59036E2 _.007 _-2274.5495 _-393.9625

42 -1253.39200* _4.59036E2 _.011 _-2193.6855 _-313.0985

49 -818.74800 4.59036E2 .085 -1759.0415 121.5455 56 -2663.45800* _4.59036E2 _.000 _-3603.7515 _-1723.1645

21 14 328.51000 4.59036E2 .480 -611.7835 1268.8035 28 -60.64800 4.59036E2 .896 -1000.9415 879.6455 35 -1005.74600* _4.59036E2 _.037 _-1946.0395 _-65.4525

42 -924.88200 4.59036E2 .054 -1865.1755 15.4115 49 -490.23800 4.59036E2 .295 -1430.5315 450.0555 56 -2334.94800* _4.59036E2 _.000 _-3275.2415 _-1394.6545

28 14 389.15800 4.59036E2 .404 -551.1355 1329.4515 21 60.64800 4.59036E2 .896 -879.6455 1000.9415 35 -945.09800* _4.59036E2 _.049 _-1885.3915 _-4.8045

42 -864.23400 4.59036E2 .070 -1804.5275 76.0595 49 -429.59000 4.59036E2 .357 -1369.8835 510.7035 56 -2274.30000* _4.59036E2 _.000 _-3214.5935 _-1334.0065

35 14 1334.25600* _4.59036E2 _.007 _393.9625 _2274.5495

21 1005.74600* _4.59036E2 _.037 _65.4525 _1946.0395

28 945.09800* _4.59036E2 _.049 _4.8045 _1885.3915

42 80.86400 4.59036E2 .861 -859.4295 1021.1575 49 515.50800 4.59036E2 .271 -424.7855 1455.8015 56 -1329.20200* _4.59036E2 _.007 _-2269.4955 _-388.9085

(24)

52

42 14 1253.39200* _4.59036E2 _.011 _313.0985 _2193.6855

21 924.88200 4.59036E2 .054 -15.4115 1865.1755 28 864.23400 4.59036E2 .070 -76.0595 1804.5275 35 -80.86400 4.59036E2 .861 -1021.1575 859.4295 49 434.64400 4.59036E2 .352 -505.6495 1374.9375 56 -1410.06600* _4.59036E2 _.005 _-2350.3595 _-469.7725

49 14 818.74800 4.59036E2 .085 -121.5455 1759.0415 21 490.23800 4.59036E2 .295 -450.0555 1430.5315 28 429.59000 4.59036E2 .357 -510.7035 1369.8835 35 -515.50800 4.59036E2 .271 -1455.8015 424.7855 42 -434.64400 4.59036E2 .352 -1374.9375 505.6495 56 -1844.71000* _4.59036E2 _.000 _-2785.0035 _-904.4165

56 14 2663.45800* _4.59036E2 _.000 _1723.1645 _3603.7515

21 2334.94800* _4.59036E2 _.000 _1394.6545 _3275.2415

28 2274.30000* _4.59036E2 _.000 _1334.0065 _3214.5935

35 1329.20200* _4.59036E2 _.007 _388.9085 _2269.4955

42 1410.06600* _4.59036E2 _.005 _469.7725 _2350.3595

49 1844.71000* _4.59036E2 _.000 _904.4165 _2785.0035

(25)

Lampiran 15. Hasil Analisis ANOVA Lebar Sirip Perut (

abdominal fin

)

Post-larva Ikan Nilem (

O. hasselti

C. V.) (µm) Perlakuan Kontrol

Umur 3-8 minggu

ANOVA

lebar sirip abdominal

Sum of Squares df Mean Square F Sig. Between Groups 9598963.976 5 1919792.795 14.492 .000 Within Groups 3179326.755 24 132471.948

Total 1.278E7 29

Post Hoc Tests

Multiple Comparisons

lebar sirip abdominal

LSD

35 -424.53600 2.30193E2 .078 -899.6308 50.5588 42 -424.53600 2.30193E2 .078 -899.6308 50.5588 49 -394.21200 2.30193E2 .100 -869.3068 80.8828 56 -1763.84600* _2.30193E2 _.000 _-2238.9408 _-1288.7512

28 21 252.70000 2.30193E2 .283 -222.3948 727.7948 35 -171.83600 2.30193E2 .463 -646.9308 303.2588 42 -171.83600 2.30193E2 .463 -646.9308 303.2588 49 -141.51200 2.30193E2 .545 -616.6068 333.5828 56 -1511.14600* _2.30193E2 _.000 _-1986.2408 _-1036.0512

35 21 424.53600 2.30193E2 .078 -50.5588 899.6308 28 171.83600 2.30193E2 .463 -303.2588 646.9308 42 .00000 2.30193E2 1.000 -475.0948 475.0948 49 30.32400 2.30193E2 .896 -444.7708 505.4188 56 -1339.31000* _2.30193E2 _.000 _-1814.4048 _-864.2152

42 21 424.53600 2.30193E2 .078 -50.5588 899.6308 28 171.83600 2.30193E2 .463 -303.2588 646.9308 35 .00000 2.30193E2 1.000 -475.0948 475.0948 49 30.32400 2.30193E2 .896 -444.7708 505.4188 56 -1339.31000* _2.30193E2 _.000 _-1814.4048 _-864.2152

49 21 394.21200 2.30193E2 .100 -80.8828 869.3068 28 141.51200 2.30193E2 .545 -333.5828 616.6068 35 -30.32400 2.30193E2 .896 -505.4188 444.7708 42 -30.32400 2.30193E2 .896 -505.4188 444.7708

(26)

54

56 -1369.63400* _2.30193E2 _.000 _-1844.7288 _-894.5392

56 21 1763.84600* _2.30193E2 _.000 _1288.7512 _2238.9408

28 1511.14600* _2.30193E2 _.000 _1036.0512 _1986.2408

35 1339.31000* _2.30193E2 _.000 _864.2152 _1814.4048

42 1339.31000* _2.30193E2 _.000 _864.2152 _1814.4048

49 1369.63400* _2.30193E2 _.000 _894.5392 _1844.7288

(27)

Lampiran 16. Hasil Analisis ANOVA Lebar Sirip Dubur (

anal fin

) Post-larva

Ikan Nilem (

O. hasselti

C. V.) (µm) Perlakuan Kontrol Umur 2-8

minggu

ANOVA

lebar sirip anal

Total 5615974.981 34

Post Hoc Tests

Multiple Comparisons

lebar sirip anal

LSD

28 -323.45600* _1.33593E2 _.022 _-597.1097 _-49.8023

35 -470.02200* _1.33593E2 _.002 _-743.6757 _-196.3683

42 -464.96800* _1.33593E2 _.002 _-738.6217 _-191.3143

49 -712.61400* _1.33593E2 _.000 _-986.2677 _-438.9603

56 -1197.79800* _1.33593E2 _.000 _-1471.4517 _-924.1443

21 14 257.75400 1.33593E2 .064 -15.8997 531.4077 28 -65.70200 1.33593E2 .627 -339.3557 207.9517 35 -212.26800 1.33593E2 .123 -485.9217 61.3857 42 -207.21400 1.33593E2 .132 -480.8677 66.4397 49 -454.86000* _1.33593E2 _.002 _-728.5137 _-181.2063

56 -940.04400* _1.33593E2 _.000 _-1213.6977 _-666.3903

28 14 323.45600* _1.33593E2 _.022 _49.8023 _597.1097

21 65.70200 1.33593E2 .627 -207.9517 339.3557 35 -146.56600 1.33593E2 .282 -420.2197 127.0877 42 -141.51200 1.33593E2 .299 -415.1657 132.1417 49 -389.15800* _1.33593E2 _.007 _-662.8117 _-115.5043

56 -874.34200* _1.33593E2 _.000 _-1147.9957 _-600.6883

35 14 470.02200* _1.33593E2 _.002 _196.3683 _743.6757

21 212.26800 1.33593E2 .123 -61.3857 485.9217 28 146.56600 1.33593E2 .282 -127.0877 420.2197 42 5.05400 1.33593E2 .970 -268.5997 278.7077 49 -242.59200 1.33593E2 .080 -516.2457 31.0617 56 -727.77600* _1.33593E2 _.000 _-1001.4297 _-454.1223

(28)

56

42 14 464.96800* _1.33593E2 _.002 _191.3143 _738.6217

21 207.21400 1.33593E2 .132 -66.4397 480.8677 28 141.51200 1.33593E2 .299 -132.1417 415.1657 35 -5.05400 1.33593E2 .970 -278.7077 268.5997 49 -247.64600 1.33593E2 .074 -521.2997 26.0077 56 -732.83000* _1.33593E2 _.000 _-1006.4837 _-459.1763

49 14 712.61400* _1.33593E2 _.000 _438.9603 _986.2677

21 454.86000* _1.33593E2 _.002 _181.2063 _728.5137

28 389.15800* _1.33593E2 _.007 _115.5043 _662.8117

35 242.59200 1.33593E2 .080 -31.0617 516.2457 42 247.64600 1.33593E2 .074 -26.0077 521.2997 56 -485.18400* _1.33593E2 _.001 _-758.8377 _-211.5303

56 14 1197.79800* _1.33593E2 _.000 _924.1443 _1471.4517

21 940.04400* _1.33593E2 _.000 _666.3903 _1213.6977

28 874.34200* _1.33593E2 _.000 _600.6883 _1147.9957

35 727.77600* _1.33593E2 _.000 _454.1223 _1001.4297

42 732.83000* _1.33593E2 _.000 _459.1763 _1006.4837

49 485.18400* _1.33593E2 _.001 _211.5303 _758.8377

(29)

Lampiran 17. Hasil Analisis ANOVA Lebar Sirip Ekor (

caudal fin

) Post-larva

Ikan Nilem (

O. hasselti

C. V.) (µm) Perlakuan Kontrol Umur 2-8

minggu

ANOVA

lebar sirip caudal

Total 3.754E7 34

Post Hoc Tests

Multiple Comparisons

lebar sirip caudal

LSD

28 -1177.58200* _3.06472E2 _.001 _-1805.3610 _-549.8030

35 -1516.20000* _3.06472E2 _.000 _-2143.9790 _-888.4210

42 -1167.47400* _3.06472E2 _.001 _-1795.2530 _-539.6950

49 -1233.17600* _3.06472E2 _.000 _-1860.9550 _-605.3970

56 -3093.04800* _3.06472E2 _.000 _-3720.8270 _-2465.2690

21 14 166.78200 3.06472E2 .591 -460.9970 794.5610 28 -1010.80000* _3.06472E2 _.003 _-1638.5790 _-383.0210

35 -1349.41800* _3.06472E2 _.000 _-1977.1970 _-721.6390

42 -1000.69200* _3.06472E2 _.003 _-1628.4710 _-372.9130

49 -1066.39400* _3.06472E2 _.002 _-1694.1730 _-438.6150

56 -2926.26600* _3.06472E2 _.000 _-3554.0450 _-2298.4870

28 14 1177.58200* _3.06472E2 _.001 _549.8030 _1805.3610

21 1010.80000* _3.06472E2 _.003 _383.0210 _1638.5790

35 -338.61800 3.06472E2 .279 -966.3970 289.1610 42 10.10800 3.06472E2 .974 -617.6710 637.8870 49 -55.59400 3.06472E2 .857 -683.3730 572.1850 56 -1915.46600* _3.06472E2 _.000 _-2543.2450 _-1287.6870

35 14 1516.20000* _3.06472E2 _.000 _888.4210 _2143.9790

21 1349.41800* _3.06472E2 _.000 _721.6390 _1977.1970

28 338.61800 3.06472E2 .279 -289.1610 966.3970 42 348.72600 3.06472E2 .265 -279.0530 976.5050 49 283.02400 3.06472E2 .364 -344.7550 910.8030 56 -1576.84800* _3.06472E2 _.000 _-2204.6270 _-949.0690

(30)

58

42 14 1167.47400* _3.06472E2 _.001 _539.6950 _1795.2530

21 1000.69200* _3.06472E2 _.003 _372.9130 _1628.4710

28 -10.10800 3.06472E2 .974 -637.8870 617.6710 35 -348.72600 3.06472E2 .265 -976.5050 279.0530 49 -65.70200 3.06472E2 .832 -693.4810 562.0770 56 -1925.57400* _3.06472E2 _.000 _-2553.3530 _-1297.7950

49 14 1233.17600* _3.06472E2 _.000 _605.3970 _1860.9550

21 1066.39400* _3.06472E2 _.002 _438.6150 _1694.1730

28 55.59400 3.06472E2 .857 -572.1850 683.3730 35 -283.02400 3.06472E2 .364 -910.8030 344.7550 42 65.70200 3.06472E2 .832 -562.0770 693.4810 56 -1859.87200* _3.06472E2 _.000 _-2487.6510 _-1232.0930

56 14 3093.04800* _3.06472E2 _.000 _2465.2690 _3720.8270

21 2926.26600* _3.06472E2 _.000 _2298.4870 _3554.0450

28 1915.46600* _3.06472E2 _.000 _1287.6870 _2543.2450

35 1576.84800* _3.06472E2 _.000 _949.0690 _2204.6270

42 1925.57400* _3.06472E2 _.000 _1297.7950 _2553.3530

49 1859.87200* _3.06472E2 _.000 _1232.0930 _2487.6510

(31)

Lampiran 18. Hasil Analisis ANOVA Luas Sirip Punggung (

dorsal fin

)

Post-larva Ikan Nilem (

O. hasselti

C. V.) (µm) Perlakuan Kontrol

Umur 2-8 minggu

ANOVA

luas sirip dorsal

Sum of Squares df Mean Square F Sig. Between Groups 1.076E15 6 1.794E14 23.384 .000 Within Groups 2.148E14 28 7.671E12

Total 1.291E15 34

Post Hoc Tests

Multiple Comparisons

luas sirip dorsal

LSD

95% Confidence Interval Lower Bound Upper Bound 14 21 -2.00512E6 1.75168E6 .262 -5.5933E6 1.5830E6

28 -2.93105E6 1.75168E6 .105 -6.5192E6 657110.1567 35 -6.79442E6* _1.75168E6 _.001 _-1.0383E7 _-3.2063E6

42 -6.41127E6* _1.75168E6 _.001 _-9.9994E6 _-2.8231E6

49 -7.94385E6* _1.75168E6 _.000 _-1.1532E7 _-4.3557E6

56 -1.82504E7* _1.75168E6 _.000 _-2.1839E7 _-1.4662E7

21 14 2.00512E6 1.75168E6 .262 -1.5830E6 5.5933E6 28 -9.25931E5 1.75168E6 .601 -4.5141E6 2.6622E6 35 -4.78930E6* _1.75168E6 _.011 _-8.3775E6 _-1.2011E6

42 -4.40615E6* _1.75168E6 _.018 _-7.9943E6 _-8.1799E5

49 -5.93873E6* _1.75168E6 _.002 _-9.5269E6 _-2.3506E6

56 -1.62453E7* _1.75168E6 _.000 _-1.9833E7 _-1.2657E7

28 14 2.93105E6 1.75168E6 .105 -6.5711E5 6.5192E6 21 9.25931E5 1.75168E6 .601 -2.6622E6 4.5141E6 35 -3.86337E6* _1.75168E6 _.036 _-7.4515E6 _-2.7521E5

42 -3.48022E6 1.75168E6 .057 -7.0684E6 107937.4627 49 -5.01280E6* _1.75168E6 _.008 _-8.6010E6 _-1.4246E6

56 -1.53194E7* _1.75168E6 _.000 _-1.8908E7 _-1.1731E7

35 14 6.79442E6* _1.75168E6 _.001 _3.2063E6 _1.0383E7

21 4.78930E6* _1.75168E6 _.011 _1.2011E6 _8.3775E6

28 3.86337E6* _1.75168E6 _.036 _275206.2773 _7.4515E6

42 3.83144E5 1.75168E6 .828 -3.2050E6 3.9713E6 49 -1.14943E6 1.75168E6 .517 -4.7376E6 2.4387E6 56 -1.14560E7* _1.75168E6 _.000 _-1.5044E7 _-7.8678E6

(32)

60

42 14 6.41127E6* _1.75168E6 _.001 _2.8231E6 _9.9994E6

21 4.40615E6* _1.75168E6 _.018 _817993.2423 _7.9943E6

28 3.48022E6 1.75168E6 .057 -1.0794E5 7.0684E6 35 -3.83144E5 1.75168E6 .828 -3.9713E6 3.2050E6 49 -1.53257E6 1.75168E6 .389 -5.1207E6 2.0556E6 56 -1.18391E7* _1.75168E6 _.000 _-1.5427E7 _-8.2510E6

49 14 7.94385E6* _1.75168E6 _.000 _4.3557E6 _1.1532E7

21 5.93873E6* _1.75168E6 _.002 _2.3506E6 _9.5269E6

28 5.01280E6* _1.75168E6 _.008 _1.4246E6 _8.6010E6

35 1.14943E6 1.75168E6 .517 -2.4387E6 4.7376E6 42 1.53257E6 1.75168E6 .389 -2.0556E6 5.1207E6 56 -1.03066E7* _1.75168E6 _.000 _-1.3895E7 _-6.7184E6

56 14 1.82504E7* _1.75168E6 _.000 _1.4662E7 _2.1839E7

21 1.62453E7* _1.75168E6 _.000 _1.2657E7 _1.9833E7

28 1.53194E7* _1.75168E6 _.000 _1.1731E7 _1.8908E7

35 1.14560E7* _1.75168E6 _.000 _7.8678E6 _1.5044E7

42 1.18391E7* _1.75168E6 _.000 _8.2510E6 _1.5427E7

49 1.03066E7* _1.75168E6 _.000 _6.7184E6 _1.3895E7

(33)

Lampiran 19. Hasil Analisis ANOVA Luas Sirip Perut (

abdominal fin

)

Post-larva Ikan Nilem (

O. hasselti

C. V.) (µm) Perlakuan Kontrol

Umur 3-8 minggu

ANOVA

luas sirip abdominal

Total 1.722E14 29

Post Hoc Tests

Multiple Comparisons

luas sirip abdominal

LSD

95% Confidence Interval Lower Bound Upper Bound 21 28 -3.95915E5 6.34162E5 .538 -1.7048E6 912931.1656

35 -1.81355E6* _6.34162E5 _.009 _-3.1224E6 _-5.0470E5

42 -1.57728E6* _6.34162E5 _.020 _-2.8861E6 _-2.6843E5

49 -1.92210E6* _6.34162E5 _.006 _-3.2310E6 _-6.1326E5

56 -6.78164E6* _6.34162E5 _.000 _-8.0905E6 _-5.4728E6

28 21 3.95915E5 6.34162E5 .538 -9.1293E5 1.7048E6 35 -1.41763E6* _6.34162E5 _.035 _-2.7265E6 _-1.0879E5

42 -1.18136E6 6.34162E5 .075 -2.4902E6 127486.4986 49 -1.52619E6* _6.34162E5 _.024 _-2.8350E6 _-2.1734E5

56 -6.38573E6* _6.34162E5 _.000 _-7.6946E6 _-5.0769E6

35 21 1.81355E6* _6.34162E5 _.009 _504700.6724 _3.1224E6

28 1.41763E6* _6.34162E5 _.035 _108785.4744 _2.7265E6

42 2.36272E5 6.34162E5 .713 -1.0726E6 1.5451E6 49 -1.08557E5 6.34162E5 .866 -1.4174E6 1.2003E6 56 -4.96810E6* _6.34162E5 _.000 _-6.2769E6 _-3.6593E6

42 21 1.57728E6* _6.34162E5 _.020 _268428.6994 _2.8861E6

28 1.18136E6 6.34162E5 .075 -1.2749E5 2.4902E6 35 -2.36272E5 6.34162E5 .713 -1.5451E6 1.0726E6 49 -3.44829E5 6.34162E5 .592 -1.6537E6 964016.9976 56 -5.20437E6* _6.34162E5 _.000 _-6.5132E6 _-3.8955E6

49 21 1.92210E6* _6.34162E5 _.006 _613258.0654 _3.2310E6

28 1.52619E6* _6.34162E5 _.024 _217342.8674 _2.8350E6

35 1.08557E5 6.34162E5 .866 -1.2003E6 1.4174E6 42 3.44829E5 6.34162E5 .592 -9.6402E5 1.6537E6

(34)

62

56 -4.85954E6* _6.34162E5 _.000 _-6.1684E6 _-3.5507E6

56 21 6.78164E6* _6.34162E5 _.000 _5.4728E6 _8.0905E6

28 6.38573E6* _6.34162E5 _.000 _5.0769E6 _7.6946E6

35 4.96810E6* _6.34162E5 _.000 _3.6593E6 _6.2769E6

42 5.20437E6* _6.34162E5 _.000 _3.8955E6 _6.5132E6

49 4.85954E6* _6.34162E5 _.000 _3.5507E6 _6.1684E6

(35)

Lampiran 20. Hasil Analisis ANOVA Luas Sirip Dubur (

anal fin

) Post-larva

Ikan Nilem (

O. hasselti

C. V.) (µm) Perlakuan Kontrol Umur 2-8

minggu

ANOVA

luas sirip anal

Total 1.109E14 34

Post Hoc Tests

Multiple Comparisons

luas sirip anal

LSD

95% Confidence Interval Lower Bound Upper Bound 14 21 -6.83273E5 5.10351E5 .191 -1.7287E6 362132.8730

28 -1.26437E6* _5.10351E5 _.020 _-2.3098E6 _-2.1897E5

35 -2.20308E6* _5.10351E5 _.000 _-3.2485E6 _-1.1577E6

42 -2.42658E6* _5.10351E5 _.000 _-3.4720E6 _-1.3812E6

49 -2.75863E6* _5.10351E5 _.000 _-3.8040E6 _-1.7132E6

56 -5.39594E6* _5.10351E5 _.000 _-6.4413E6 _-4.3505E6

21 14 6.83273E5 5.10351E5 .191 -3.6213E5 1.7287E6 28 -5.81101E5 5.10351E5 .265 -1.6265E6 464304.5370 35 -1.51980E6* _5.10351E5 _.006 _-2.5652E6 _-4.7440E5

42 -1.74330E6* _5.10351E5 _.002 _-2.7887E6 _-6.9790E5

49 -2.07536E6* _5.10351E5 _.000 _-3.1208E6 _-1.0300E6

56 -4.71267E6* _5.10351E5 _.000 _-5.7581E6 _-3.6673E6

28 14 1.26437E6* _5.10351E5 _.020 _218968.4660 _2.3098E6

21 5.81101E5 5.10351E5 .265 -4.6430E5 1.6265E6 35 -9.38702E5 5.10351E5 .076 -1.9841E6 106703.7130 42 -1.16220E6* _5.10351E5 _.031 _-2.2076E6 _-1.1680E5

49 -1.49426E6* _5.10351E5 _.007 _-2.5397E6 _-4.4885E5

56 -4.13157E6* _5.10351E5 _.000 _-5.1770E6 _-3.0862E6

35 14 2.20308E6* _5.10351E5 _.000 _1.1577E6 _3.2485E6

21 1.51980E6* _5.10351E5 _.006 _474397.6260 _2.5652E6

28 9.38702E5 5.10351E5 .076 -1.0670E5 1.9841E6 42 -2.23501E5 5.10351E5 .665 -1.2689E6 821905.3610 49 -5.55558E5 5.10351E5 .286 -1.6010E6 489847.4530 56 -3.19286E6* _5.10351E5 _.000 _-4.2383E6 _-2.1475E6

(36)

64

42 14 2.42658E6* _5.10351E5 _.000 _1.3812E6 _3.4720E6

21 1.74330E6* _5.10351E5 _.002 _697898.1410 _2.7887E6

28 1.16220E6* _5.10351E5 _.031 _116796.8020 _2.2076E6

35 2.23501E5 5.10351E5 .665 -8.2191E5 1.2689E6 49 -3.32058E5 5.10351E5 .521 -1.3775E6 713347.9680 56 -2.96936E6* _5.10351E5 _.000 _-4.0148E6 _-1.9240E6

49 14 2.75863E6* _5.10351E5 _.000 _1.7132E6 _3.8040E6

21 2.07536E6* _5.10351E5 _.000 _1.0300E6 _3.1208E6

28 1.49426E6* _5.10351E5 _.007 _448854.7100 _2.5397E6

35 5.55558E5 5.10351E5 .286 -4.8985E5 1.6010E6 42 3.32058E5 5.10351E5 .521 -7.1335E5 1.3775E6 56 -2.63731E6* _5.10351E5 _.000 _-3.6827E6 _-1.5919E6

56 14 5.39594E6* _5.10351E5 _.000 _4.3505E6 _6.4413E6

21 4.71267E6* _5.10351E5 _.000 _3.6673E6 _5.7581E6

28 4.13157E6* _5.10351E5 _.000 _3.0862E6 _5.1770E6

35 3.19286E6* _5.10351E5 _.000 _2.1475E6 _4.2383E6

42 2.96936E6* _5.10351E5 _.000 _1.9240E6 _4.0148E6

49 2.63731E6* _5.10351E5 _.000 _1.5919E6 _3.6827E6

(37)

Lampiran 21. Hasil Analisis ANOVA Luas Sirip Ekor (

caudal fin

) Post-larva

Ikan Nilem (

O. hasselti

C. V.) (µm) Perlakuan Kontrol Umur 2-8

minggu

ANOVA

luas sirip caudal

Total 1.910E15 34

Post Hoc Tests

Multiple Comparisons

luas sirip caudal

LSD

95% Confidence Interval Lower Bound Upper Bound 14 21 -2.93744E6* _1.04232E6 _.009 _-5.0725E6 _-8.0233E5

28 -7.84806E6* _1.04232E6 _.000 _-9.9832E6 _-5.7130E6

35 -1.24586E7* _1.04232E6 _.000 _-1.4594E7 _-1.0323E7

42 -1.27331E7* _1.04232E6 _.000 _-1.4868E7 _-1.0598E7

49 -1.21010E7* _1.04232E6 _.000 _-1.4236E7 _-9.9659E6

56 -2.39656E7* _1.04232E6 _.000 _-2.6101E7 _-2.1831E7

21 14 2.93744E6* _1.04232E6 _.009 _802334.8140 _5.0725E6

28 -4.91063E6* _1.04232E6 _.000 _-7.0457E6 _-2.7755E6

35 -9.52112E6* _1.04232E6 _.000 _-1.1656E7 _-7.3860E6

42 -9.79571E6* _1.04232E6 _.000 _-1.1931E7 _-7.6606E6

49 -9.16352E6* _1.04232E6 _.000 _-1.1299E7 _-7.0284E6

56 -2.10282E7* _1.04232E6 _.000 _-2.3163E7 _-1.8893E7

28 14 7.84806E6* _1.04232E6 _.000 _5.7130E6 _9.9832E6

21 4.91063E6* _1.04232E6 _.000 _2.7755E6 _7.0457E6

35 -4.61050E6* _1.04232E6 _.000 _-6.7456E6 _-2.4754E6

42 -4.88508E6* _1.04232E6 _.000 _-7.0202E6 _-2.7500E6

49 -4.25290E6* _1.04232E6 _.000 _-6.3880E6 _-2.1178E6

56 -1.61176E7* _1.04232E6 _.000 _-1.8253E7 _-1.3982E7

35 14 1.24586E7* _1.04232E6 _.000 _1.0323E7 _1.4594E7

21 9.52112E6* _1.04232E6 _.000 _7.3860E6 _1.1656E7

28 4.61050E6* _1.04232E6 _.000 _2.4754E6 _6.7456E6

42 -2.74586E5 1.04232E6 .794 -2.4097E6 1.8605E6 49 3.57601E5 1.04232E6 .734 -1.7775E6 2.4927E6 56 -1.15071E7* _1.04232E6 _.000 _-1.3642E7 _-9.3720E6

(38)

66

42 14 1.27331E7* _1.04232E6 _.000 _1.0598E7 _1.4868E7

21 9.79571E6* _1.04232E6 _.000 _7.6606E6 _1.1931E7

28 4.88508E6* _1.04232E6 _.000 _2.7500E6 _7.0202E6

35 2.74586E5 1.04232E6 .794 -1.8605E6 2.4097E6 49 6.32187E5 1.04232E6 .549 -1.5029E6 2.7673E6 56 -1.12325E7* _1.04232E6 _.000 _-1.3368E7 _-9.0974E6

49 14 1.21010E7* _1.04232E6 _.000 _9.9659E6 _1.4236E7

21 9.16352E6* _1.04232E6 _.000 _7.0284E6 _1.1299E7

28 4.25290E6* _1.04232E6 _.000 _2.1178E6 _6.3880E6

35 -3.57601E5 1.04232E6 .734 -2.4927E6 1.7775E6 42 -6.32187E5 1.04232E6 .549 -2.7673E6 1.5029E6 56 -1.18647E7* _1.04232E6 _.000 _-1.4000E7 _-9.7296E6

56 14 2.39656E7* _1.04232E6 _.000 _2.1831E7 _2.6101E7

21 2.10282E7* _1.04232E6 _.000 _1.8893E7 _2.3163E7

28 1.61176E7* _1.04232E6 _.000 _1.3982E7 _1.8253E7

35 1.15071E7* _1.04232E6 _.000 _9.3720E6 _1.3642E7

42 1.12325E7* _1.04232E6 _.000 _9.0974E6 _1.3368E7

49 1.18647E7* _1.04232E6 _.000 _9.7296E6 _1.4000E7

(39)

Lampiran 22. Hasil Analisis ANOVA Jumlah

Ray

Sirip Punggung (

dorsal fin

)

Post-larva Ikan Nilem (

O. hasselti

C. V.) (µm) Perlakuan

Kontrol Umur 2-8 minggu

ANOVA

jumlah ray sirip dorsal

Total 84.400 34

Post Hoc Tests

Multiple Comparisons

jumlah ray sirip dorsal

LSD

95% Confidence Interval Lower Bound Upper Bound 14 21 -2.20000* _.72111 _.005 _-3.6771 _-.7229

28 -1.60000* _.72111 _.035 _-3.0771 _-.1229

35 -.60000 .72111 .412 -2.0771 .8771 42 .60000 .72111 .412 -.8771 2.0771 49 -1.60000* _.72111 _.035 _-3.0771 _-.1229

56 -3.00000* _.72111 _.000 _-4.4771 _-1.5229

21 14 2.20000* _.72111 _.005 _.7229 _3.6771

28 .60000 .72111 .412 -.8771 2.0771 35 1.60000* _.72111 _.035 _.1229 _3.0771

42 2.80000* _.72111 _.001 _1.3229 _4.2771

49 .60000 .72111 .412 -.8771 2.0771 56 -.80000 .72111 .277 -2.2771 .6771 28 14 1.60000* _.72111 _.035 _.1229 _3.0771

21 -.60000 .72111 .412 -2.0771 .8771 35 1.00000 .72111 .176 -.4771 2.4771 42 2.20000* _.72111 _.005 _.7229 _3.6771

49 .00000 .72111 1.000 -1.4771 1.4771 56 -1.40000 .72111 .062 -2.8771 .0771 35 14 .60000 .72111 .412 -.8771 2.0771 21 -1.60000* _.72111 _.035 _-3.0771 _-.1229

28 -1.00000 .72111 .176 -2.4771 .4771 42 1.20000 .72111 .107 -.2771 2.6771 49 -1.00000 .72111 .176 -2.4771 .4771 56 -2.40000* _.72111 _.002 _-3.8771 _-.9229

(40)

68

42 14 -.60000 .72111 .412 -2.0771 .8771 21 -2.80000* _.72111 _.001 _-4.2771 _-1.3229

28 -2.20000* _.72111 _.005 _-3.6771 _-.7229

35 -1.20000 .72111 .107 -2.6771 .2771 49 -2.20000* _.72111 _.005 _-3.6771 _-.7229

56 -3.60000* _.72111 _.000 _-5.0771 _-2.1229

49 14 1.60000* _.72111 _.035 _.1229 _3.0771

21 -.60000 .72111 .412 -2.0771 .8771 28 .00000 .72111 1.000 -1.4771 1.4771 35 1.00000 .72111 .176 -.4771 2.4771 42 2.20000* _.72111 _.005 _.7229 _3.6771

56 -1.40000 .72111 .062 -2.8771 .0771 56 14 3.00000* _.72111 _.000 _1.5229 _4.4771

21 .80000 .72111 .277 -.6771 2.2771 28 1.40000 .72111 .062 -.0771 2.8771 35 2.40000* _.72111 _.002 _.9229 _3.8771

42 3.60000* _.72111 _.000 _2.1229 _5.0771

49 1.40000 .72111 .062 -.0771 2.8771