Proceedings of the 15th AAAP Animal Science Congress 26-30 November 2012, Thammasat University, Rangsit Campus, Thailand
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C15-OP-157 Effects of BTN and DGAT1 Genes on Milk Production and Quality Traits in Thai-Holstein Dairy Crossbred
S. Cheunsuk and J. Sanpote (Thailand)
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C15-OP-158 The Interleukin-8 Gene Polymorphism and Its Association with Milk Production Traits in Holstein Cattle
C15-OP-160 Differentially Expressed Genes in the Liver of High and Low Feed Efficiency Bulls
P. F. Arthur, Y. Chen, R. M. Herd, K. Quinn and I. M. Barchia (Australia)
843
C15-OP-161 Genetic Architecture and Population Structure of Hanwoo Cattle
C. Gondro, G. W. Jang, S. H. Lee, S. H. Yeon and H. H. Seong (Australia)
848
C15-OP-162 Associations between Estrogen Receptor Gene Polymorphisms and Fertility in Kabinburi Cows
K. Boonyanuwat, A. Puttaratanang, Y. Lephaichit and P. Luangmaneewech (Thailand)
854
C15-OP-163 Polymorphisms of the Prion Protein Gene (PRNP) in Thai Indigenous Cattle
K. Boonyanuwat, C. Dongpalee, S. Saithong and W. Kamsung (Thailand)
858
C15-OP-164 Genetics Potency of Madura Cattle as Local Indonesian Breed in Madura Island, Indonesia
V. M. A. Nurgiartiningsih, G. Ciptadi, B. Hartono and S. Siswijono (Indonesia)
865
C15-OP-165 Beyond Genome Wide Association Tests in the Livestock Industry: Genetic Architecture of Growth and Carcass Weight in Hanwoo
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and Growth Traits of Limousin Cross Madura Cattle T. Hartatik, S. D. Volkandari and Sumadi (Indonesia)
C15-OP-168 Studying Molecular Markers of Twinning Births in Indonesian Local Beef Cattle and Its Crossbreds A. Anggraeni, S. Arta, H. Hasinah, C. Talib and B. Tiesnamurti (Indonesia)
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C15-OP-169 Identification of Single Nucleotide Polymorphisms in OLR1
Gene Affecting Milk Production in Pakistani Cattle
M. E. Babar, A. Ali, N. Majeed, A. Nadeem and T. Hussain (Pakistan)
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C15-OP-170 Ecat1, A Maternal Effect Gene in Bovine Ovary, Oocyte and
In Vitro Embryo
A. Zahmatkesh, M. D. Joupari, S. A. Mahyari, A. Shirazi and M. A. Roudbar (Iran)
896
C15-OP-171 Growth of Five Breed of Sheep under Station Conditions Subandriyo, E. Handiwirawan, B. Setiadi and I. Inounu (Indonesia)
901
C16-OP-172 Analysis on the Development of the Smallholders Dairying Di Pasuruan Regency, East Java - Indonesia
S. B. Siswijono, I. Subagiyo and V. M. A. Nurgiartiningsih (Indonesia)
905
C16-OP-173 Developing a Novel Method to Cooling Dairy Cattle
T. L. Sy, K. G. Gebremedhin, J. E. Larson, J. Davis and C. N. Lee (USA)
909
C16-OP-174 Farmers’ Skill and Linkage to Milk Market Influence the
Prevalence of Anoestrus in Postpartum Crossbred Cows in Bangladesh
M. M. Kamal, G. Opsomer, N. Parveen, H. W. Momont and M. Shamsuddin (Bangladesh)
914
C16-OP-175 Estimating the Sustainability of Bali Cattle Farming Systems on Ceram Island, Indonesia, by Applying Locally Derived Indicators
F. Attamimi, M. Siegmund-Schultze and A. V. Zárate (Indonesia)
921
C16-OP-176 Factors Affecting the Farm Gate Selling Price of Smallholder Beef Cattle in East Java, Indonesia
A. Priyanti, I. G. A. P. Mahendri, F. Cahyadi and R. A. Cramb (Indonesia)
Proceedings of the 15th AAAP Animal Science Congress 26-30 November 2012, Thammasat University, Rangsit Campus, Thailand
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Relatedness between Polymorphism Growth Hormone Gene and Growth Traits of Limousin Cross Madura Cattle
T. Hartatik, S. D. Volkandari and Sumadi
Faculty of Animal Science, Universitas Gadjah Mada, Bulaksumur, Yogyakarta, Indonesia
The research was conducted to know the genotype of Limousin cross Madura cattle (Limura) and investigate the effect of polymorphism growth hormone (GH) gene for growth traits. The growth traits were examined based on quantitative data which contain birth weight and body weight at day 60, average daily gain (ADG), body length, heart girth, withers height and hip height. The sample for the experiment consist of 35 Limura calves and 10 Madura calves. Analysis of polymorphism GH gene were using Polymerase Chain Reaction-Restriction Fragment Lenght Polymorphism (PCR-RFLP) method. The PCR product of GH gene in this research (211 bp) was digested by AluI enzyme. The relatedness between genotype and growth traits were analyzed using one way ANOVA, where three genotype groups (LL_Limura, LV_Limura, and LL_Madura) as factors. The results indicated that the genotype of Madura cattle were 100% LL and the genotype of Limura cattle were LL (82.86%) and LV (17.14%). There are no significant differences of birth weight and pre-weaning body weight in three groups of genotype. However, both genotype LL and LV of Limura cattle had greater ADG, body length at birth and pre-weaning hip height gain than those of LL_Madura genotypes (p<0.05). In conclusion, the polymorphism GH gene does not affect the growth traits, but the differences of two breeds (Limura dan Madura cattle) seem influence the growth trait of the cattle.
Key Words: Genotype, GH gene, Growth traits, Limousin cross Madura cattle (Limura)
INTRODUCTION
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polymorphism GH gene in Limura and Madura cattle as a control. This molecular approach intends to be an introductory step for genetic characterization of these indigenous cattle (Madura) and the crossbreed (Limura). The relationship between polimorphisme GH gene and growth traits is very important to know the potential of local cattle and the offspring of crossbreed cattle.
MATERIALS AND METHODS
Sample and measurement of growth traits
The sample of the research were consist of 35 Limousin cross Madura calves and 10 Madura calves. Body weight and body size was measured at the birth ( day 1) and 60 days of age (day 60). The body size was measured using a tape FH and ruler FHK brand. The body size data including: body length, heart girth, withers height and hip height. The body length (absolut) is
the straight distance that measured between Tuberculum lateralis to Tuberculum ischiadicum.
Heart girth is the length of the circular (the circumference) as measured on chest that right in the back of withers on the rib (costae) 3-4. Withers height is straight distance from the plane to the highest point in withers. Hip height was obtained by measuring the distance from the plane until the highest point of the hip. Data of body measurement was taken with parallelogram position of the livestock.
Detection of the polymorphism growth hormone gene
DNA sample was extracted from blood sample using SDS/ProteinaseK modified methode from Sambrook et al (1989). A 211 bp fragment of GH was amplified by polymerase chain
reaction (PCR) which refer to Reis etal.(2001). The PCR reaction contains 1 μl DNA sample,
1 μl each primer (10 pmol), 10 μl PCR KIT (Fastart, Roche) and 7 μl aquabidest in total solution 20 μl. Amplification in vitro was conducted for 35 cycles using GeneAmp® PCR
System 9700 (Applied Biosystems™). The PCR product (5μl) was digested with 1 units AluI
enzyme (Fermentas) in a total volume 20 μl and incubated at 37ºCfor 3 hours. The
polymorphism GH gene was analyzed by separating restriction product in vertical electrophoresis (BioRad) with 12% polyacrilamide gel (PAGE), 1X TBE buffer and running at 50 Voltage for 3 hour 30 minutes.
Data analysis
Proceedings of the 15th AAAP Animal Science Congress 26-30 November 2012, Thammasat University, Rangsit Campus, Thailand
883
RESULTS AND DISCUSSION
Based on PCR-RFLP methods, we characterized the associations between genetic polymorphisms in GH gene and growth traits of Limura and Madura cattle. The result of genotyping GH gene of Limura cattle were 82.86% LL and 17.14% LV, whereas Madura cattle were 100% LL. The relationship between genotype and growth traits can be seen in Table 1-3. In this study, we measured and evaluated for ADG, body weight, body lenght, heart girth, withers height, and hip height at day 1 and day 60 of calves.
Table 1 Body weight (kg) and average daily gain (kg/day) of Limura and Madura cattle
Parameter Limura (LL) (n=29) Limura (LV) (n=6) Madura (LL)
(n=10)
Birth weight (day 1) 22.73 ± 4.59 a 21.96 ± 5.61 a 20.55 ± 3.43 a
Body weight day 60 55.39 ± 12.96 a 55.79 ± 18.65 a 44.39 ± 5.78 a
Average daily gain 0.54 ± 0.16 a 0.56 ± 0.24a 0.39 ± 0.09b
Superscript a,b at the same row indicate significantly different (p<0,05)
The results indicated that both genotype LL and LV of Limura cattle had greater ADG, body length at day 1 and hip height gain than those with genotypes LL of Madura (p<0.05). The two genotype within Limura breed does not show the significant different in all growth traits. Average calf birth weight of Madura cattle and crossbreed was greater than that of Bali cattle ( 16 kg for females and 17.5 kg for males). The pre-weaning ADG for Bali cattle was 0.4 kg/day (Julianto et al., 2010). Thus, Limura cattle had ADG higher than that of Bali cattle and Madura cattle. Its seem that Madura and Bali cattle had the same pre-weaning ADG.
Table 2 Body measurement of Limura and Madura cattle at day 1 and day 60 (cm)
Parameter Limura (LL) (n=29) Limura (LV) (n=6) Madura (LL) (n=10)
Day 1:
Body length 55.56 ± 4.74a 54.43 ± 3.03a 49.04 ± 2.03b
Heart girth 67.08 ± 5.29a 66.85 ± 3.31a 64.85 ± 2.76a
Withers height 65.75 ± 5.09a 66.51 ± 4.81a 66.42 ± 2.96a
Hip height 69.36 ± 5.19a 69.53 ± 4.83a 69.61 ± 4.34a
Day 60:
Body length 72.95 ± 5.50 a 73.34 ± 5.66 a 68.28 ± 7.88 a
Heart girth 87.27 ± 6.95 a 87.47 ± 7.49 a 81.74 ± 4.28 a
Withers height 79.10 ± 4.90 a 79.52 ± 5.75 a 79.57 ± 4.38 a
Hip height 84.83 ± 6.43 a 84.21 ± 7.66 a 80.14 ± 4.50 a
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Table 3 Body measurement gain of Limura and Madura (cm/day)
Parameter Limura (LL) (n=29) Limura (LV) (n=6) Madura (LL) (n=10)
Body length gain 0.29 ± 0.09 a 0.31 ± 0.09 a 0.32 ± 0.13 a
Heart girth gain 0.34 ± 0.08 a 0.34 ± 0.08 a 0.28 ± 0.05 a
Withers height gain 0.22 ± 0.05 a 0.22 ± 0.06 a 0.22 ± 0.05 a
Hip height gain 0.26 ± 0.08 a 0.24 ± 0.12 a 0.17 ± 0.06 b
Superscript a,b at the same row indicate significantly different (p<0,05)
In conclusion, the polymorphism GH gene does not affect the growth traits, but the differences of two breeds (Limura dan Madura cattle) seem influence the growth trait of the cattle.
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