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Lampiran 1 Analisis deskriptif pada lima kelompok sampel ikan tuna mata besar

DESCRIPTIVE STATISTICS

********************************************************

SAMPLE GROUP 1

Locus 1 # obs. at locus= 50

allele: # obs: allele freq: # hets: het freq: 1 1 0.0200 0.0000 0.0000 2 25 0.5000 0.0000 0.0000 3 7 0.1400 0.0000 0.0000 4 2 0.0400 0.0000 0.0000 5 7 0.1400 0.0000 0.0000 6 1 0.0200 0.0000 0.0000 7 1 0.0200 0.0000 0.0000 8 2 0.0400 0.0000 0.0000 9 1 0.0200 0.0000 0.0000 10 1 0.0200 0.0000 0.0000 11 1 0.0200 0.0000 0.0000 12 1 0.0200 0.0000 0.0000 13 0 0.0000 0.0000 0.0000 14 0 0.0000 0.0000 0.0000 15 0 0.0000 0.0000 0.0000 16 0 0.0000 0.0000 0.0000 17 0 0.0000 0.0000 0.0000 18 0 0.0000 0.0000 0.0000 19 0 0.0000 0.0000 0.0000 20 0 0.0000 0.0000 0.0000 21 0 0.0000 0.0000 0.0000 22 0 0.0000 0.0000 0.0000 23 0 0.0000 0.0000 0.0000 Heterozygosity: 0.7048 Heterozygosity (unbiased): 0.7119 Heterozygosity (direct count): 0.0000

--- Results over all loci

Ave. sample size: 50.0000 Ave. heterozygosity: 0.7048

Ave. heterozygosity (unbiased): 0.7119 Ave. heterozygosity (direct count): 0.0000 % polymorphic loci (no criterion): 100.0000 % polymorphic loci (99% criterion): 100.0000 % polymorphic loci (95% criterion): 100.0000 ---

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52

Lanjutan Lampiran 1

SAMPLE GROUP 2

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Lanjutan Lampiran 1

SAMPLE GROUP 3

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54

Lanjutan Lampiran 1

SAMPLE GROUP 4

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Lanjutan Lampiran 1

SAMPLE GROUP 5

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56

Lampiran 2 Analisis jarak genetik pada lima kelompok sampel ikan tuna mata besar

GENETIC DISTANCES

NEI'S (1972/1978) IDENTITIES/DISTANCES

Populations unbiased unbiased compared dist. ident. dist. ident. 1 vs. 2 0.0596 0.9421 0.0341 0.9665 1 vs. 3 0.3545 0.7015 0.3241 0.7232 1 vs. 4 0.1840 0.8319 0.1371 0.8719 1 vs. 5 0.0589 0.9428 0.0363 0.9644 2 vs. 3 0.5145 0.5978 0.4830 0.6169 2 vs. 4 0.2178 0.8043 0.1698 0.8439 2 vs. 5 0.0383 0.9625 0.0145 0.9856 3 vs. 4 0.1615 0.8508 0.1086 0.8971 3 vs. 5 0.6621 0.5158 0.6334 0.5308 4 vs. 5 0.3261 0.7218 0.2809 0.7551

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Lampiran 3 Analisis keragaman lima kelompok sampel ikan tuna mata besar

Exact tests for population differentiation (Raymond and Rousset 1995) # of dememorization steps: 1000

# of batches: 10

# of permutations per batch: 2000

Pairwise analysis of all populations

Groups compared: 1 vs. 2

locus 1 : p = 0.1266 S.E. :0.0174 Results over loci not calculated

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58

Lampiran 4 Distribusi haplotipe pada lima kelompok sampel ikan tuna mata besar

KELOMPOK SAMPEL 1

Enzim

Taq l Afa l Mbo l Hin6 l

A B C D E A B C D E A B C D E A B C D E Komposit haplotipe 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 AAAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 BAAB 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 BAAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 BBAA 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 BABA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 BBAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 BBAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 BBAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 BBAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0 BBCA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 BBAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 BBCB 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 ABCB 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 BBBA 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 0 1 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 CBAA 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 0 0 0 BCAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 BBAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 BBCB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB

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Lanjutan Lampiran 4

KELOMPOK SAMPEL 2

Enzim

A B C D E A B C D E A B C D E A B C D E Komposit haplotipe 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 BAAA 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 BAAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 ABAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 BAAB 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 BAAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 BBCB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 BBAC 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 BBCB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 BBAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 BBAA 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 BBCB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 ABAD 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 BBCB 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 BBCB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 BBAA 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 BBCB

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60

Lanjutan Lampiran 4

KELOMPOK SAMPEL 3

Enzim

A B C D E A B C D E A B C D E A B C D E Komposit haplotipe 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 ABCB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 1 0 0 0 0 BADA 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 BBBA 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 ABCB 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 BAAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 BAAA 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 BBCB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 BBAC 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 ABCB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 BBBA 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 BBCB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 ABAB

(19)

Lanjutan Lampiran 4

KELOMPOK SAMPEL 4

Enzim

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62

Lanjutan Lampiran 4

KELOMPOK SAMPEL 5

Enzim

A B C D E A B C D E A B C D E A B C D E Komposit haplotipe 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 BEAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 BBAC 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0 BBCA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 BBAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 BBAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 BBAA 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 BBCB 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 BAAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 BBAC 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 BBAC 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 BBCB 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 BBAB