Lampiran 3: Bagan Penelitian Ulangan I Ulangan II Ulangan III

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Seleksi : Seleksi lini murni, tiga generasi asal segregate alamiah Daya hasil : 1,6–2,5 t/ha

Warna hipokotil : Ungu

Warna bulu : Coklat kekuningan Warna bunga : Ungu

Warna kulit biji : Kuning Warna hilum : Terang

Bentuk daun : Oblong, ujung runcing Tipe tumbuh : Determinit

Umur berbunga : 35 hari Umur polong matang : 80–82 hari Tinggi tanaman : 60–70 cm Percabangan : 1–2 cabang Bobot 100 biji : 17 g Ukuran biji : Besar Kandungan protein : 39% Kandungan minyak : 20%

Kerebahan : Tidak mudah rebah Ketahanan terhadap

penyakit : Toleran karat daun

Keterangan : Sesuai untuk bahan baku susu kedelai, tempe, dan tahu

Pemulia : Rodiah S., Ono Sutrisno, Gatot Kustiyono, Sumarno, dan Soegito

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Lampiran 2. Jadwal Penelitian.

Jenis Kegiatan Minggu

ke-1 2 3 4 5 6 7 8 9 10

Pembiakan serangga uji X X X X Penyediaan jamur B.

bassiana danM. anisopliae

X X

Pembuatan suspensi B. Bassianadan M. Anisopliae

X X X

Persiapan media tanam X

Pemupukan X

Penanaman benih X

Pemeliharaan tanaman X X X

Pengaplikasian serangga

ke tanaman X

Pengaplikasian Suspensi B. Bassianadan M. Anisopliae

X

Penyungkupan X X X

Peubah Amatan

Persentase Mortalitas ulat

grayak X X

Intensitas serangan X X

Waktu larva mati

X X

Persentase larva menjadi

pupa X

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Keterangan :

P0 : Kontrol

P1 : Suspensi Metharizium anisopliaedengan Kerapatan konidia 104/50 ml air. P2 : Suspensi Metharizium anisopliaedengan Kerapatan konidia 106/50 ml air. P3 : SuspensiMetharizium anisopliaedengan Kerapatan konidia 108/50 ml air. P4 : SuspensiBeauveria bassiana dengan Kerapatan konidia 104/50 ml air. P5 : Suspensi Beauveria bassiana dengan Kerapatan konidia 106/50 ml air. P6 : Suspensi Beauveria bassiana dengan Kerapatan konidia 108/50 ml air

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Lampiran 4. Data Persentase mortalitas larva 1 hsa

Data mentah mortalitas 1 hsa

Perlakuan Ulangan

1 2 3

% mortalitas 1 hsa

Perlakuan Blok Total Rataan

1.00 2.00 3.00

P0 0.00 0.00 0.00 0.00 0.00

Total 0.00 0.00 0.00 0.00

Rataan 0.00 0.00 0.00 0.00

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P1 1 1 2

% mortalitas 2 hsa

1.00 2.00 3.00

P0 0.00 0.00 0.00 0.00 0.00

P1 20.00 20.00 40.00 80.00 26.67

P2 40.00 20.00 20.00 80.00 26.67

P3 40.00 40.00 40.00 120.00 40.00

P4 20.00 0.00 20.00 40.00 13.33

P5 20.00 20.00 40.00 80.00 26.67

P6 20.00 20.00 40.00 80.00 26.67

Total 160.00 120.00 200.00 480.00

Rataan 22.86 17.14 28.57 22.86

Transformasi Arcsin √persentase

1.00 2.00 3.00

P0 4.05 4.05 4.05 12.16 4.05

P1 26.57 26.57 39.23 92.36 30.79

P2 39.23 26.57 26.57 92.36 30.79

P3 39.23 39.23 39.23 117.69 39.23

P4 26.57 4.05 26.57 57.18 19.06

P5 26.57 26.57 39.23 92.36 30.79

P6 26.57 26.57 39.23 92.36 30.79

Total 188.78 153.60 214.11 556.49

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Daftar Sidik Ragam

SK db JK KT F.hit F 0,05 F 0,01 Ket

Blok 2.00 263.84 131.92 3.15 3.89 6.93 tn

P 6.00 2384.18 397.36 9.50 3.00 4.82 **

Galat 12.00 501.81 41.82

Total 20.00 3149.83

FK= 14746.74

SY 3.73 -11.50 1.29 14.30 14.09 13.94 13.83 27.09

I 2.00 3.00 4.00 5.00 6.00 7.00 8.00

SSR 0.05 3.08 3.23 3.31 3.37 3.41 3.44 3.46

LSR 0.05 11.50 12.04 12.37 12.58 12.73 12.84 12.91

Perlakuan P0 P4 P1 P2 P5 P6 P3

Rataan 0.00 13.33 26.67 26.67 26.67 26.67 40.00

a b

c d

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P1 2 2 2

% mortalitas 3 hsa

1.00 2.00 3.00

P0 0.00 0.00 0.00 0.00 0.00

P1 40.00 40.00 40.00 120.00 40.00

P2 40.00 60.00 40.00 140.00 46.67

P3 60.00 60.00 40.00 160.00 53.33

P4 40.00 20.00 40.00 100.00 33.33

P5 40.00 40.00 60.00 140.00 46.67

P6 40.00 20.00 60.00 120.00 40.00

Total 260.00 240.00 280.00 780.00

Rataan 37.14 34.29 40.00 37.14

1.00 2.00 3.00

P0 4.05 4.05 4.05 12.16 4.05

P1 39.23 39.23 39.23 117.69 39.23

P2 39.23 50.77 39.23 129.23 43.08

P3 50.77 50.77 39.23 140.77 46.92

P4 39.23 26.57 39.23 105.03 35.01

P5 39.23 39.23 50.77 129.23 43.08

P6 39.23 26.57 50.77 116.57 38.86

Total 250.98 237.18 262.52 750.68

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SK Db JK KT F.hit F 0,05 F 0,01 Ket

Blok 2.00 45.96 22.98 0.44 3.89 6.93 tn

P 6.00 3777.31 629.55 12.18 3.00 4.82 **

Galat 12.00 620.32 51.69 Total 20.00 4443.59

FK= 26834.57

SY 4.15 -12.79 19.94 26.25 26.01 32.51 32.39 38.97

I 2.00 3.00 4.00 5.00 6.00 7.00 8.00

SSR 0.05 3.08 3.23 3.31 3.37 3.41 3.44 3.46

LSR 0.05 12.79 13.39 13.75 13.99 14.16 14.28 14.36

Rataan 0.00 33.33 40.00 40.00 46.67 46.67 53.33

a b

c

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P1 2 2 3

% mortalitas 4 hsa

1.00 2.00 3.00

P0 0.00 0.00 0.00 0.00 0.00

P1 40.00 40.00 60.00 140.00 46.67 P2 60.00 40.00 60.00 160.00 53.33 P3 60.00 80.00 40.00 180.00 60.00 P4 40.00 20.00 60.00 120.00 40.00 P5 40.00 40.00 60.00 140.00 46.67 P6 40.00 60.00 60.00 160.00 53.33 Total 280.00 280.00 340.00 900.00

Rataan 40.00 40.00 48.57 42.86

Transformasi Arcsin √persentase

1.00 2.00 3.00

P0 4.05 4.05 4.05 12.16 4.05

P1 39.23 39.23 50.77 129.23 43.08 P2 50.77 39.23 50.77 140.77 46.92 P3 50.77 63.43 39.23 153.43 51.14 P4 39.23 26.57 50.77 116.57 38.86 P5 39.23 39.23 50.77 129.23 43.08 P6 39.23 50.77 50.77 140.77 46.92 Total 262.52 262.52 297.13 822.16

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Daftar Sidik Ragam

Blok 2.00 114.09 57.04 0.83 3.89 6.93 tn

P 6.00 4581.95 763.66 11.08 3.00 4.82 **

Galat 12.00 827.08 68.92

Total 20.00 5523.12

FK= 32188.30

SY 4.79 -14.77 24.54 30.80 30.52 36.99 36.85 43.42

I 2.00 3.00 4.00 5.00 6.00 7.00 8.00

SSR 0.05 3.08 3.23 3.31 3.37 3.41 3.44 3.46

LSR 0.05 14.77 15.46 15.87 16.15 16.34 16.48 16.58

Rataan 0.00 40.00 46.67 46.67 53.33 53.33 60.00

a b

c

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P1 3 3 3

% mortalitas 5 hsa

1.00 2.00 3.00

P0 0.00 0.00 0.00 0.00 0.00

P1 60.00 60.00 60.00 180.00 60.00

P2 60.00 60.00 80.00 200.00 66.67

P3 80.00 80.00 60.00 220.00 73.33

P4 40.00 40.00 60.00 140.00 46.67

P5 60.00 60.00 60.00 180.00 60.00

P6 60.00 80.00 60.00 200.00 66.67

Total 360.00 380.00 380.00 1120.00

Rataan 51.43 54.29 54.29 53.33

1.00 2.00 3.00

P0 4.05 4.05 4.05 12.16 4.05

P1 50.77 50.77 50.77 152.31 50.77

P2 50.77 50.77 63.43 164.97 54.99

P3 63.43 63.43 50.77 177.64 59.21

P4 39.23 39.23 50.77 129.23 43.08

P5 50.77 50.77 50.77 152.31 50.77

P6 50.77 63.43 50.77 164.97 54.99

Total 309.80 322.46 321.33 953.59

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SK db JK KT F.hit F 0,05 F 0,01 Ket

Blok 2.00 14.04 7.02 0.21 3.89 6.93 tn

P 6.00 6441.64 1073.61 32.57 3.00 4.82 **

Galat 12.00 395.57 32.96

Total 20.00 6851.26

FK= 43301.52

SY 3.31 -10.21 35.98 49.02 48.83 55.37 55.27 61.86

I 2.00 3.00 4.00 5.00 6.00 7.00 8.00

SSR 0.05 3.08 3.23 3.31 3.37 3.41 3.44 3.46

LSR 0.05 10.21 10.69 10.98 11.17 11.30 11.40 11.47

Rataan 0.00 46.67 60.00 60.00 66.67 66.67 73.33

a b

c d

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P1 3 4 3

% mortalitas 6 hsa

1.00 2.00 3.00

P0 0.00 0.00 0.00 0.00 0.00

P1 60.00 80.00 60.00 200.00 66.67

P2 80.00 60.00 80.00 220.00 73.33

P3 100.00 100.00 100.00 300.00 100.00

P4 60.00 60.00 80.00 200.00 66.67

P5 60.00 80.00 80.00 220.00 73.33

P6 80.00 80.00 100.00 260.00 86.67

Total 440.00 460.00 500.00 1400.00

Rataan 62.86 65.71 71.43 66.67

1.00 2.00 3.00

P0 4.05 4.05 4.05 12.16 4.05

P1 50.77 63.43 50.77 164.97 54.99

P2 63.43 50.77 63.43 177.64 59.21

P3 90.00 90.00 90.00 270.00 90.00

P4 50.77 50.77 63.43 164.97 54.99

P5 50.77 63.43 63.43 177.64 59.21

P6 63.43 63.43 90.00 216.87 72.29

Total 373.23 385.90 425.13 1184.25

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SK db JK KT F.hit F 0,05 F 0,01 ket

Blok 2.00 209.19 104.59 1.82 3.89 6.93 tn

P 6.00 12423.80 2070.63 36.06 3.00 4.82 **

Galat 12.00 689.12 57.43

Total 20.00 13322.10

FK= 66783.79

SY 4.38 -13.48 52.56 52.18 58.59 58.41 71.62 84.87

I 2.00 3.00 4.00 5.00 6.00 7.00 8.00

SSR 0.05 3.08 3.23 3.31 3.37 3.41 3.44 3.46

LSR 0.05 13.48 14.11 14.49 14.74 14.92 15.05 15.13

Rataan 0.00 66.67 66.67 73.33 73.33 86.67 100.00

a b

c d

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P0 0 0 0

% mortalitas 7 hsa

1.00 2.00 3.00

P0 0.00 0.00 0.00 0.00 0.00

P1 80.00 80.00 60.00 220.00 73.33

P2 80.00 80.00 80.00 240.00 80.00

P3 100.00 100.00 100.00 300.00 100.00

P4 80.00 60.00 80.00 220.00 73.33

P5 80.00 80.00 80.00 240.00 80.00

P6 80.00 100.00 100.00 280.00 93.33

Total 500.00 500.00 500.00 1500.00

Rataan 71.43 71.43 71.43 71.43

1.00 2.00 3.00

P0 4.05 4.05 4.05 12.16 4.05

P1 63.43 63.43 50.77 177.64 59.21

P2 63.43 63.43 63.43 190.30 63.43

P3 90.00 90.00 90.00 270.00 90.00

P4 63.43 50.77 63.43 177.64 59.21

P5 63.43 63.43 63.43 190.30 63.43

P6 63.43 90.00 90.00 243.43 81.14

Total 411.23 425.13 425.13 1261.49

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Blok 2.00 18.40 9.20 0.17 3.89 6.93 tn

P 6.00 13505.33 2250.89 40.56 3.00 4.82 **

Galat 12.00 665.99 55.50

Total 20.00 14189.72

FK= 75778.40

SY 4.30 -13.25 59.46 59.08 65.51 65.33 78.54 85.12

I 2.00 3.00 4.00 5.00 6.00 7.00 8.00

SSR 0.05 3.08 3.23 3.31 3.37 3.41 3.44 3.46

LSR 0.05 13.25 13.87 14.25 14.49 14.67 14.79 14.88

Rataan 0.00 73.33 73.33 80.00 80.00 93.33 100.00

a b

c d

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P1 1 1 2

1 2 3

P0 100.00 100.00 100.00 300.00 100.00

P1 20.00 20.00 20.00 60.00 20.00

P2 20.00 20.00 0.00 40.00 13.33

P3 0.00 0.00 0.00 0.00 0.00

P4 20.00 40.00 20.00 80.00 26.67

P5 20.00 20.00 20.00 60.00 20.00

P6 20.00 0.00 0.00 20.00 6.67

Total 200.00 200.00 160.00 560.00

Rataan 28.57 28.57 22.86 26.67

1 2 3

P0 90.00 90.00 90.00 270.00 90.00

P1 26.57 26.57 26.57 79.70 26.57

P2 26.57 26.57 0.00 53.13 17.71

P3 4.05 4.05 4.05 12.16 4.05

P4 26.57 39.23 26.57 92.36 30.79

P5 26.57 26.57 26.57 79.70 26.57

P6 26.57 4.05 4.05 34.67 11.56

Total 226.88 217.04 177.80 621.72

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Blok 2.00 9.23 4.61 0.10 3.89 6.93 tn

P 6.00 13868.65 2311.44 51.13 3.00 4.82 **

Galat 12.00 542.50 45.21

Total 20.00 14420.37

FK= 20802.78

SY 3.88 -11.96 -5.85 7.14 6.92 13.43 13.32 86.57

I 2.00 3.00 4.00 5.00 6.00 7.00 8.00

SSR 0.05 3.08 3.23 3.31 3.37 3.41 3.44 3.46

LSR 0.05 11.96 12.52 12.86 13.08 13.24 13.35 13.43

Rataan 0.00 6.67 20.00 20.00 26.67 26.67 100.00

a b

c

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P0 17.60 20.00 16.10 53.70 17.90 P1 16.60 15.00 13.20 44.80 14.93 P2 16.00 11.70 11.60 39.30 13.10

P3 8.80 10.00 10.00 28.80 9.60

P4 16.10 16.60 13.20 45.90 15.30 P5 15.00 10.20 11.70 36.90 12.30

P6 11.60 8.80 10.20 30.60 10.20

Total 101.70 92.30 86.00 280.00

Rataan 14.53 13.19 12.29 13.33

Transformasi √X+0.5

1 2 3

P0 4.25 4.53 4.07 12.86 4.29

P1 4.14 3.94 3.70 11.77 3.92

P2 4.06 3.49 3.48 11.03 3.68

P3 3.05 3.24 3.24 9.53 3.18

P4 4.07 4.14 3.70 11.91 3.97

P5 3.94 3.27 3.49 10.70 3.57

P6 3.48 3.05 3.27 9.80 3.27

Total 26.99 25.65 24.96 77.60

Rataan 3.86 3.66 3.57 3.70

Blok 2.00 0.30 0.15 3.19 3.89 6.93 tn

P 6.00 2.84 0.47 9.92 3.00 4.82 **

Galat 12.00 0.57 0.05

Total 20.00 3.71

FK= 286.79

KK= 0.06

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Uji Jarak Duncan

SY 0.13 9.21 9.79 9.78 11.88 14.50 14.87 17.46

I 2.00 3.00 4.00 5.00 6.00 7.00 8.00

SSR 0.05 3.08 3.23 3.31 3.37 3.41 3.44 3.46

LSR 0.05 0.39 0.41 0.42 0.42 0.43 0.43 0.44

Rataan 9.60 10.20 12.30 13.10 14.93 15.30 17.90

a

b

c

d

e

f

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P0 26.00 22.00 28.00 76.00 25.33 P1 17.00 17.60 20.50 55.10 18.37 P2 16.10 16.00 17.60 49.70 16.57 P3 14.70 15.00 16.60 46.30 15.43 P4 17.00 20.00 22.00 59.00 19.67 P5 16.60 18.30 19.10 54.00 18.00 P6 15.00 13.20 18.30 46.50 15.50 Total 122.40 122.10 142.10 386.60

Rataan 17.49 17.44 20.30 18.41

Transformasi √X+0.5

1 2 3

P0 5.15 4.74 5.34 15.23 5.08

P1 4.18 4.25 4.58 13.02 4.34

P2 4.07 4.06 4.25 12.39 4.13

P3 3.90 3.94 4.14 11.97 3.99

P4 4.18 4.53 4.74 13.45 4.48

P5 4.14 4.34 4.43 12.90 4.30

P6 3.94 3.70 4.34 11.97 3.99

Total 29.56 29.56 31.82 90.94

Rataan 4.22 4.22 4.55 4.33

Blok 2.00 0.48 0.24 11.37 3.89 6.93 **

P 6.00 2.56 0.43 19.98 3.00 4.82 **

Galat 12.00 0.26 0.02 Total 20.00 3.30

FK= 393.80

KK= 0.03

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Uji Jarak Duncan

SY 0.08 15.17 15.23 16.29 17.72 18.08 19.38 25.04

I 2.00 3.00 4.00 5.00 6.00 7.00 8.00

SSR 0.05 3.08 3.23 3.31 3.37 3.41 3.44 3.46

LSR 0.05 0.26 0.27 0.28 0.28 0.29 0.29 0.29

Rataan 15.43 15.50 16.57 18.00 18.37 19.67 25.33

a

b

c

d

e

f

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P1 0 0 0

Data Persentase Mortalitas 2 Hsa

Perlakuan Ulangan

1 2 3

Perlakuan Ulangan

1 2 3

Perlakuan Ulangan

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Perlakuan Ulangan

1 2 3

Perlakuan Ulangan

1 2 3

Perlakuan Ulangan

1 2 3

waktu kematian

Perlakuan hari

ke-1 2 3 4 5 6 7

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s

Foto pupa S. lituraF. (Kontrol) Foto gejala serangan larva

S. lituraF.

Foto larva S. lituraF. yang terinfeksi M. anisopliae(104)

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Foto larva S. lituraF._yang

terinfeksiB. bassiana_.

Foto larva S. lituraF._{yang tidak}

terinfeksi Foto larva S. lituraF. _yang

terinfeksi B. bassiana ₍₁₀6₎

Foto larva S. lituraF. _yang

terinfeksi B. bassiana ₍₁₀8₎