Pengaruh ekstrak temu mangga (Curcuma mangga Val et Zyp) terhadap kadar kolesterol total, kolesterol HDL, kolesterol LDL dan trigliserid pada tikus putih jantan - Widya Mandala Catholic University Surabaya Repository

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Lampiran 1 Perhitungan Penetapan Kadar Abu Serbuk Rimpang Temu Mangga

Replikasi W (krus + abu ) (g)

W krus

kosong W abu (g) W simplisia Kadar abu 1

2 3

19,1730 19,1741 19,1738

19,0421 19,0423 19,0426

0,1309 0,1318 0,1312

2,0140 1,9987 2,0101

6,5 6,5 6,6

W (krus + abu) (g) – w kurs kosong (g) Kadar abu (%) =

W simplisia x 100%

Perhitungan surut Pengeringan Serbuk Rimpang Temu Mangga

Replikasi

Hasil Surut Pengeringan 1

2 3

7,2 7,4 7,2

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Lampiran 2

Hasil Uji Pemantapan Mutu Reagen Diagnostik dan Alat Pengukur

Serum kontrol Nilai hasil pemeriksaan

Rentang nilai yang diperkenankan

Keterangan

Normal 90,5 mg/dl (79,3 – 107,5) mg/dl Sesuai Patologis 209 mg/dl (171 – 223) mg/dl Sesuai

Senyawa Larut Etanol

Berat ekstrak Berat filtrat kering Senyawa larut etanol

5,1520 0,4150 8,0 %

0,4150 % Senyawa larut etanol =

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Y2 ij

Lampiran 3 PERHITUNGAN ANAVA

KOLESTEROL HARI KE 15

P E R L A K U A N JUMLAH

K- 15% 20% 25% K+ JUMLAH

1 54 45 50 35 49

2 45 56 56 52 60

3 53 58 44 44 38

4 54 59 39 58 57

5 41 61 44 42 35

n 5 5 5 5 5 25

RATA-RATA 49.40 55.80 46.60 46.20 47.80 -

Ji 247 279 233 231 239 1229

Ji2 61009 77841 54289 53361 57121 303621 Perhitungan JK :

k = 5 n = 5 N = 25

∑ = 62015

J = ∑Ji = 1229

JK TOTAL = ∑ – = 1597.360

∑J J2 JK. Py =

n – N = 306.560

JK. Ey = TOTAL – Py = 1290.800 J2

N

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TABEL ANAVA

SV db JK RJK F hit. F (0.05)

Py 4 306.5600 76.6400 1.1875 2.8661

Ey 20 1290.8000 64.5400

TOTAL 24 1597.3600 - - -

db (TOTAL) = kn – 1 RJK = JK / db

db (Py) = (k – 1) F hitung = RJK (Py) / RJK (Ey) db (Ey) = db (TOTAL) – Py)

Kriteria Pengujian :

- Bila F hitung > F (0.05) maka berbeda signifikan PENGUJIAN HIPOTESIS :

a. H : Pi = 0

Yang berarti tidak ada perbedaan EFEK yang signifikan sebagai akibat perbedaan perlakuan.

b. KESIMPULAN :

Karena F hitung < F (0.05) maka H diterima.

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Lampiran 4

UJI TUCKEY (HSD)

PERL

MEAN

K- 49.40

0.15 55.80

0.2 46.60

0.25 46.20

K+ 47.80 K- 49.40 0.00 6.40 ts 2.80 ts 3.20 ts 1.60 ts 0.15 55.80 0.00 9.20 9.60 ts 8.00 ts

0.2 46.60 0.00 0.40 ts 1.20 ts

0.25 46.20 0.00 1.60 ts

K+ 47.80 0.00

RJK = 64.540 q(5%/2; p;db) = 4.24

n = 5 HSD (5%) = q

n RJK

= 15.33 db = 20

Keterangan:

(6)

Y2 ij

KOLESTEROL HARI KE 30

K- 15% 20% 25% K+ JUMLAH

1 3 21 35 37 51

2 2 19 32 40 50

3 3 25 30 42 39

4 4 26 29 46 53

5 4 17 28 39 41

n 5 5 5 5 5 25

RATA-RATA 3.20 21.60 30.80 40.80 46.80 -

Ji 16 108 154 204 234 716

k = 5 n = 5 N = 25

∑ = 26702

J = ∑Ji = 716

JK TOTAL = ∑ – = 6195.760

∑J J2 JK. Py =

n – N = 5895.360 JK. Ey = TOTAL – Py = 300.400

J2 N

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TABEL ANAVA

Py 4 5895.3600 1473.8400 98.1252 2.8661 Ey 20 300.4000 15.0200

TOTAL 24 6195.7600 - - -

a. H : Pi = 0

b. KESIMPULAN :

Karena F hitung > F (0.05) maka H ditolak.

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Lampiran 6

PERL

MEAN

K- 3.20

0.15 21.60

0.2 30.80

0.25 40.80

K+ 46.80 K- 3.20 0.00 18.40 * 27.60 * 37.60 * 43.60 * 0.15 21.60 0.00 9.20 * 19.20 * 25.20 *

0.2 30.80 0.00 10.00 * 16.00 *

0.25 40.80 0.00 6.00 ts

K+ 46.80 0.00

RJK = 15.020 q(5%/2; p;db) = 4.24

n = 5 HSD (5%) = q 7.349

n RJK ₌

db = 20 Keterangan:

(9)

Y2 ij

LDL HARI KE 15

K- 15% 20% 25% K+ JUMLAH

1 50 41.9 47.8 28.3 42.9

2 40.2 53.2 50.5 42.3 57.8 3 47.8 53.3 39.8 42.5 35.1 4 54.6 55.1 36.9 56.4 56.4

5 34 58.6 41.2 44.7 30.7

n 5 5 5 5 5 25

RATA-RATA 45.32 52.42 43.24 42.84 44.58 -

Ji 227 262 216 214 223 1142

k = 5 n = 5 N = 25

∑ = 54025.12

J = ∑Ji = 1142

JK TOTAL = ∑ – = 1858.560

∑J J2 JK. Py =

n – N = 303.932

JK. Ey = TOTAL – Py = 1554.628 J2

N

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TABEL ANAVA

Py 4 303.9320 75.9830 0.9775 2.8661

Ey 20 1554.6280 77.7314

TOTAL 24 1858.5600 - - -

a. H : Pi = 0

b. KESIMPULAN :

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Lampiran 8

PERL

MEAN

K- 45.32

0.15 52.42

0.2 43.24

0.25 42.84

K+ 44.58

K- 45.32 0.00 7.10 ts 2.08 ts 2.48 ts 0.74 ts 0.15 52.42 0.00 9.18 ts 9.58 ts 7.84 ts

0.2 43.24 0.00 0.40 ts 1.34 ts

0.25 42.84 0.00 1.74 ts

K+ 44.58 0.00

RJK = 77.731 q(5%/2; p;db) = 4.24

n = 5 HSD (5%) = q

n RJK

= 16.718 db = 20

Keterangan:

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Y2 ij

LDL HARI KE 30

K- 15% 20% 25% K+ JUMLAH

1 5 21.2 32.9 35.2 50.5

2 1.9 19.1 31.3 37.7 48.6

3 0 23.7 29.2 39.3 36.1

4 5 27 27.6 43.8 55.1

5 4.4 16.9 25.4 38 36.7

n 5 5 5 5 5 25

RATA-RATA 3.26 21.58 29.28 38.80 45.40 -

Ji 16 108 146 194 227 692

k = 5 n = 5 N = 25

∑ = 24950.76

J = ∑Ji = 691.6

JK TOTAL = ∑ – = 5818.338

∑J J2 JK. Py =

n – N = 5368.790 JK. Ey = TOTAL – Py = 449.548

J2 N

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TABEL ANAVA

Py 4 5368.7896 1342.1974 59.7132 2.8661 Ey 20 449.5480 22.4775

TOTAL 24 5818.3376 - - -

a. H : Pi = 0

b. KESIMPULAN :

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Lampiran 10

PERL

MEAN

K- 3.26

0.15 21.58

0.2 29.28

0.25 38.80

K+ 45.40 K- 3.26 0.00 18.32 * 26.02 * 35.54 * 42.14 * 0.15 21.58 0.00 7.70 ts 17.22 * 23.82 *

0.2 29.28 0.00 6.52 * 16.12 *

0.25 38.80 0.00 6.60 ts

K+ 45.40 0.00

RJK = 22.477 q(5%/2; p;db) = 4.24

n = 5 HSD (5%) = q

n RJK

= 8.990 db = 20

Keterangan:

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Y2 ij

HDL HARI KE 15

K- 15% 20% 25% K+ JUMLAH 1 14.6 15.5 16.4 12.9 14.5 2 15.7 16.6 15.9 11.3 18.4 3 14.8 14.9 15.4 17.5 13.7

4 16.6 16.5 14.9 17 20.6

5 12.2 17.4 18.4 19.1 13.7

n 5 5 5 5 5 25

RATA-RATA 14.78 16.18 16.20 15.56 16.18 -

Ji 74 81 81 78 81 395

k = 5 n = 5 N = 25

∑ = 6338.13

J = ∑Ji = 394.5

JK TOTAL = ∑ – = 112.920

∑J J2 JK. Py =

n – N = 7.724

JK. Ey = TOTAL – Py = 105.196 J2

N

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TABEL ANAVA

Py 4 7.7240 1.9310 0.3671 2.8661

Ey 20 105.1960 5.2598

TOTAL 24 112.9200 - - -

a. H : Pi = 0

b. KESIMPULAN :

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Lampiran 12

PERL

MEAN

K- 14.78

0.15 16.18

0.2 16.20

0.25 15.56

K+ 16.18 K- 14.78 0.00 1.40 ts 1.42 ts 0.78 ts 1.40 ts 0.15 16.18 0.00 0.02 ts 0.62 ts 0.00 ts

0.2 16.20 0.00 0.64 ts 0.02 ts

0.25 15.56 0.00 0.62 ts

K+ 16.18 0.00

RJK = 5.260 q(5%/2; p;db) = 4.24

n = 5 HSD (5%) = q

n RJK

= 4.349 db = 20

Keterangan:

(18)

Y2 ij

HDL HARI KE 30

K- 15% 20% 25% K+ JUMLAH

1 12.4 12 12.9 14 16.5

2 10.5 12.5 13.5 13.5 16

3 7.6 11.7 12.8 14.3 14.1 4 11.4 12.9 13.8 13.4 20.7 5 10.8 12.3 11.4 15.4 14.1

n 5 5 5 5 5 25

RATA-RATA 10.54 12.28 12.88 14.12 16.28 -

Ji 53 61 64 71 81 331

k = 5 n = 5 N = 25

∑ = 4509.93

J = ∑Ji = 330.5

JK TOTAL = ∑ – = 140.720

∑J J2 JK. Py =

n – N = 91.776

JK. Ey = TOTAL – Py = 48.944 J2

N

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TABEL ANAVA

Py 4 91.7760 22.9440 9.3756 2.8661

Ey 20 48.9440 2.4472

TOTAL 24 140.7200 - - -

a. H : Pi = 0

b. KESIMPULAN :

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Lampiran 14

PERL

MEAN

K- 10.54

0.15 12.28

0.2 12.88

0.25 14.12

K+ 16.28

K- 10.54 0.00 1.74 ts 2.34 ts 3.58 * 5.74 * 0.15 12.28 0.00 0.60 ts 1.84 ts 4.00 *

0.2 12.88 0.00 1.24 ts 3.40 *

0.25 14.12 0.00 2.16 ts

K+ 16.28 0.00

RJK = 2.447 q(5%/2; p;db) = 4.24

n = 5 HSD (5%) = q

n RJK

= 2.996 db = 20

Keterangan:

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Y2 ij

TRIGLISERID HARI KE 15

K- 15% 20% 25% K+ JUMLAH

1 43 43 43 48 53

2 53 47 57 55 53

3 50 48 48 45 33

4 30 52 35 51 56

5 46 49 56 32 40

n 5 5 5 5 5 25

RATA-RATA 44.40 47.80 47.80 46.20 47.00 -

Ji 222 239 239 231 235 1166

k = 5 n = 5 N = 25

∑ = 55826

J = ∑Ji = 1166

JK TOTAL = ∑ – = 1443.760

∑J J2 JK. Py =

n – N = 40.160

JK. Ey = TOTAL – Py = 1403.600 J2

N

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TABEL ANAVA

Py 4 40.1600 10.0400 0.1431 2.8661

Ey 20 1403.6000 70.1800

TOTAL 24 1443.7600 - - -

a. H : Pi = 0

b. KESIMPULAN :

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Lampiran 16

PERL

MEAN

K- 44.40

0.15 47.80

0.2 47.80

0.25 46.20

K+ 47.80

K- 44.40 0.00 3.40 ts 3.40 ts 1.80 ts 2.60 ts 0.15 47.80 0.00 0.00 ts 1.60 ts 0.80 ts

0.2 47.80 0.00 1.60 ts 0.80 ts

0.25 46.20 0.00 0.80 ts

K+ 47.00 0.00

RJK = 70.180 q(5%/2; p;db) = 4.24

n = 5 HSD (5%) = q

n RJK

= 15.885 db = 20

Keterangan:

(24)

Y2 ij

TRIGLISERID HARI KE 30

K- 15% 20% 25% K+ JUMLAH

1 2 9 25 29 35

2 3 12 21 34 37

3 3 15 18 35 35

4 2 8 26 28 43

5 2 12 20 32 42

n 5 5 5 5 5 25

RATA-RATA 2.40 11.20 22.00 31.60 38.40 -

Ji 12 56 110 158 192 528

k = 5 n = 5 N = 25

∑ = 15616

J = ∑Ji = 528

JK TOTAL = ∑ – = 4464.640

∑J J2 JK. Py =

n – N = 4290.240 JK. Ey = TOTAL – Py = 174.400

J2 N

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TABEL ANAVA

Py 4 4290.2400 1072.5600 123.0000 2.8661 Ey 20 174.4000 8.7200

TOTAL 24 4464.6400 - - -

a. H : Pi = 0

b. KESIMPULAN :

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Lampiran 18

PERL

MEAN

K- 2.40

0.15 11.20

0.2 22.00

0.25 31.60

K+ 38.40

K- 2.40 0.00 8.80 * 19.60 * 29.20 * 36.00 * 0.15 11.20 0.00 10.80 * 20.40 * 27.20 *

0.2 22.00 0.00 9.60 * 16.40 *

0.25 31.60 0.00 6.80 *

K+ 38.40 0.00

RJK = 70.180 q(5%/2; p;db) = 4.24

n = 5 HSD (5%) = q

n RJK

= 15.885 db = 20

Keterangan:

(27)

Lampiran 19

Perhitungan Persen (%) Penurunan Kadar Kolesterol Total (Hari ke-30)

Kelompok No

Persen (%) Penurunan Kadar Kolesterol total

Hari ke – 30

K1

1 2 3 4 5

2,00 16,52 20,83 22,22 13,93

K₂

1 2 3 4 5

31,53 27,82 27,52 26,12 26,41

K3

1 2 3 4 5

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Persamaan garis regresi antara persen penurunan kadar kolesterol total (y) terhadap dosis ekstrak rimpang temu mangga 1,5 g, 2,0 g, 2,5 g/kg BB (x) sebagai berikut :

a = - 8,3100, b = 18,0420, r = 0,9528

y = - b x + a

y = - 18,0420 x – 8,3100

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Lampiran 20

Perhitungan Persen (%) Peningkatan Kadar Kolesterol HDL (Hari ke-30)

Kelompok No

Persen (%) Peningkatan Kadar Kolesterol HDL

Hari ke – 30

K1

1 2 3 4 5

9,43 10,28

6,22 11,15 11,50

K₂

1 2 3 4 5

11,11 14,34 11,66 14,44 6,16

K3

1 2 3 4 5

(30)

Persamaan garis regresi antara persen peningkatan kadar kolesterol HDL (y) terhadap dosis ekstrak rimpang temu mangga 1,5 g, 2,0 g, 2,5 g/kg BB (x) sebagai berikut :

a = - 0,8620, b = 6,7120,

r = 0,6986

y = - b x + a

y = - 6,7120 x – 0,8620

Kesimpulan : r hitung (0,9528) > r tabel (0,514), maka ada hubungan antara peningkatan dosis dengan efek peningkatan kadar kolesterol HDL.

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Lampiran 21

Perhitungan Persen (%) Penurunan Kadar Kolesterol LDL(Hari ke-30)

Kelompok No

Persen (%) Penurunan Kadar Kolesterol LDL

Hari ke – 30

K1

1 2 3 4 5

32,31 26,60 36,37 38,18 20,58

K₂

1 2 3 4 5

50,00 44,35 43,84 40,29 40,77

K3

1 2 3 4 5

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Persamaan garis regresi antara persen penurunan kadar kolesterol LDL (y) terhadap dosis ekstrak rimpang temu mangga 1,5 g, 2,0 g, 2,5 g/kg BB (x) sebagai berikut :

a = - 8,7146, b = 26,3220

r = 0,9231

y = - b x + a

y = - 26,3220 x – 8,7146

(33)

Lampiran 22

Perhitungan Persen (%) Penurunan Kadar Trigliserid (Hari ke-30)

Kelompok No

Persen (%) Peningkatan Kadar Trigliserid Hari ke – 30

K₁

1 2 3 4 5

8,91 11,21 14,28 7,14 10,81

K2

1 2 3 4 5

22,72 17,94 16,98 16,00 17,85

K₃

1 2 3 4 5

(34)

Persamaan garis regresi antara persen penurunan kadar Trigliserid (y) terhadap dosis ekstrak rimpang temu mangga 1,5 g, 2,0 g, 2,5 g/kg BB (x) sebagai berikut :

a = - 16,4106, b = 18,0940

r = 0,9337

y = - b x + a

y = - 18,0940 x – 16,4106

(35)

Lampiran 23 Tabel Uji HSD (0,05)

DAFTAR E

Nilai Rentang Student untuk = 0,05

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Lampiran 24 Tabel Korelasi (r)

HARGA KRITIK DARI r PRODUCT MOMENT

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Lampiran 26

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