113
Lampiran 1. Komposisi media RPMI-1640
Komponen Konsentrasi (mg/l) Garam anorganik Ca(NO3)2.4H2O 100 KCl 400 MgSO4.7H2O 100 NaCl 6000 NaHCO3 2000 Na2HPO4 anhydrous 800 Komponen lain D-Glukosa 2000 fenol merah 5,3 glutathione (tereduksi) 1 HEPES (d) 5958 Asam Amino L-arginin HCl 200 L-asparagin 56,82 L-asam aspartat 20 L-sistin 50 L-asam glutamat 20 L-glutamin 300 Glisin 10 L-histidin 15 L-hidroksiprolin 20 L-isoleusin 50 L-leusin 50 L-lisin HCl 40 L-metionin 15 L-fenilalanin 15 L-prolin 20 L-serin 30 L-threonin 20 L-triptofan 5 L-tirosin 20 L-valin 20 Vitamin D-biotin 0,2 B-Ca pantotenat 0,25 kolin klorida 3 asam folat 1 i-inositol 35
114 Lanjutan tabel 1 Komponen Konsentrasi (mg/l) nikotinamida 1 PABA 1 Piridoksal HCl 1 Riboflavin 0,2 Tiamin HCl 1 Vitamin B12 0,005
115
Lampiran 2. Komposisi balanced salt solution PBS (Harrison and Rae, 1997) Komponen Konsentrasi (mg/l) CaCl2.H2O 132,5 KCl 200 KH2PO4 200 MgCl2.6H2O 100 NaCl 8000 Na2HPO4 1150
116
Lampiran 3. Proses Produksi Pangan Iradiasi yang shelf-stable (IAEA, 1995) Langkah kunci dalam produksi pangan iradiasi adalah sebagai berikut:
1. Masaklah terlebih dahulu daging mentah, unggas, dan seafood sampai keadaan matang (medium done) dengan syarat suhu internalnya kira-kira 75°C, untuk menginaktivasi enzim pengurai jaringan, kecuali bahan yang dimasak telah diproses secara termal sebelumnya (misalnya diasapkan (hot-smoked), dikuring, atau dibuat pastrami)
2. Secara hermetis kemaslah secara vakum dalam kemasan individu yang berupa kantong multilapis alumunium foil, seperti yang dikembangkan oleh program US Army dan disetujui oleh FDA atau yang setara.
3. Bekukan produk dalam kemasan secara kriogenik atau mekanis hingga mencapai -40°C dan iradiasi saat masih dalam keadaan beku untuk mempertahankan kualitas gizi. Menumpuk kemasan dengan dry ice merupakan cara yang efektif dan tidak mahal untuk mempertahankan keadaan beku selama proses iradiasi yang panjang.
4. Untuk iradiasi gamma atau sinar X, kemas kantong-kantong pengemas individual beku dalam karton pengemas dan simpan dalam keadaan beku atau dengan dry ice sebelum diiradiasi. Jika waktu iradiasi lebih dari satu jam, dry ice harus ditaruh di sekitar karton pengemas untuk mempertahankan keadaan beku (hard frozen) selama proses iradiasi. Bahkan pada energy maksimum 10 MeV iradiasi electron /electron beam (yang memiliki daya penetrasi rendah) harus diterapkan pada tiap kantong produk yang dibekukan, misalnya: kantong yang diletakkan dalam posisi tidur pada ban berjalan yang membawa produk-produk tersebut saat diiradiasi dengan electron beam. Kantong yang berukuran lebih tebal daripada beberapa sentimeter harus diiradiasi pada tiap sisi (two-pass, 50% dari total minimum dosis pada tiap kali) untuk mencapai keseragaman. Laju dosis iradiasi dengan electron beam normalnya merupakan dosis yang sangat tinggi sehingga tidak membutuhkan penanganan aktif untuk mempertahankan keadaan beku selama iradiasi (waktu paparan normalnya
117
terlalu sebentar untuk kemungkinan terjadinya kenaikan suhu yang signifikan).
5. Lakukan proses iradiasi dengan dosis minimum yang telah ditentukan (25-45 kGy tergantung produknya) untuk memberikan tingkat 10-12 jaminan sterilitas antibotulinum dengan menggunakan dosimetri yang sesuai. Dosis minimum yang dibutuhkan untuk berbagai produk telah ditentukan oleh studi produk yang diinokulasi dengan C. botulinum yang dilakukan oleh program US Army. Pada level dosis minimum ini, produk steril secara mikrobiologi dan dapat disimpan selama bertahun-tahun dalam kondisi suhu ruang karena enzim endogen yang akan mendegradasi produk, meskipun tidak ada mikroorganisme yang hidup, telah diinaktivasi dengan panas sebelum dikemas dan diiradiasi.
6. Segera setelah iradiasi, biarkan suhu produk naik secara perlahan hingga mencapai suhu ruang. Kemas secara individu kantong-kantong produk dalam karton pengemas seperti saat sebelum diiradiasi.
118
Lampiran 4. Tabel data hasil pembacaan absorbansi uji proliferasi limfosit
Sampel 1x 2x 4x 11 Nov 06 2,170 2,338 2,436 1,592 1,099 1,693 1,666 2,052 1,553 DIPA 14 Jun 07 2,385 1,44 1,208 2,228 1,431 1,528 1,939 1,745 1,788 No label 14 Jun 07 1,882 0,783 1,324 2,777 1,478 1,303 2,161 1,612 1,887 Kontrol Rendang 1,478 1,413 1,345 1,68 1,229 1,536 1,633 1,196 1,338 K+LPS 1,155 1,504 1,377 K+PWM 1,992 1,683 0,741 K- 1,094 1,321 1,428
119
Lampiran 5. Contoh cara perhitungan untuk mendapatkan Indeks Stimulasi (I.S.) proliferasi limfosit
*Sampel 11 November 06, pengenceran 1x Diketahui : Absorbansi sampel = 2,170
Absorbansi kontrol negatif = 1,904 maka
I.S. = Abs ekstrak sampel Abs kontrol negatif
I.S. = 2,170 1,094 I.S. = 1,984
120
Lampiran 6. Tabel data hasil pembacaan absorbansi uji hemolisis eritrosit pengenceran 1x Sampel Waktu pengamatan (jam) 0 1 2 3 4 5 11 Nov 2006 1x 0,120 0,122 0,128 0,128 0,132 0,137 0,117 0,119 0,119 0,118 0,124 0,121 0,121 0,130 0,125 0,143 0,130 0,132 DIPA 14 Jun 2007 1x 0,162 0,127 0,133 0,122 0,129 0,131 0,119 0,126 0,122 0,122 0,130 0,132 0,114 0,118 0,122 0,116 0,124 0,123 No Label 14 Jun 2007 1x 0,137 0,137 0,138 0,142 0,137 0,141 0,143 0,159 0,164 0,172 0,171 0,179 Kontrol Rendang 1x 0,126 0,126 0,128 0,180 0,117 0,129 0,166 0,164 0,168 0,170 0,182 0,173 0,134 0,131 0,131 0,138 0,123 0,133 K+ 1x 0,106 0,103 0,117 0,114 0,116 0,123 0,105 0,104 0,113 0,108 0,108 0,115 0,108 0,119 0,134 0,134 0,147 0,160 K- 1x 0,095 0,101 0,107 0,106 0,111 0,115 0,105 0,122 0,132 0,138 0,151 0,162 0,107 0,105 0,113 0,110 0,117 0,121
121
Lampiran 7. Tabel data hasil pembacaan absorbansi uji hemolisis eritrosit pengenceran 2x Sampel Waktu pengamatan (jam) 0 1 2 3 4 5 11 Nov 2006 2x 0,127 0,139 0,145 0,151 0,150 0,155 0,110 0,112 0,113 0,112 0,115 0,116 0,108 0,108 0,108 0,109 0,112 0,112 DIPA 14 Jun 2007 2x 0,114 0,112 0,135 0,113 0,114 0,114 0,126 0,131 0,109 0,130 0,132 0,137 0,114 0,120 0,122 0,123 0,132 0,133 No Label 14 Jun 2007 2x 0,140 0,137 0,137 0,132 0,142 0,140 0,120 0,115 0,118 0,112 0,117 0,120 0,119 0,113 0,116 0,108 0,112 0,112 Kontrol Rendang 2x 0,122 0,131 0,141 0,130 0,158 0,144 0,119 0,108 0,117 0,111 0,112 0,114 0,134 0,142 0,146 0,146 0,149 0,153
122
Lampiran 8. Tabel data hasil pembacaan absorbansi uji hemolisis eritrosit pengenceran 4x Sampel Waktu pengamatan (jam) 0 1 2 3 4 5 11 Nov 2006 4x 0,122 0,136 0,159 0,200 0,200 0,226 0,109 0,107 0,106 0,111 0,110 0,108 0,110 0,101 0,102 0,103 0,106 0,106 DIPA 14 Jun 2007 4x 0,115 0,107 0,113 0,112 0,111 0,115 0,111 0,106 0,111 0,115 0,106 0,106 0,099 0,100 0,103 0,104 0,104 0,104 No Label 14 Jun 2007 4x 0,109 0,119 0,116 0,113 0,119 0,120 0,106 0,118 0,112 0,111 0,118 0,124 0,108 0,109 0,113 0,106 0,113 0,115 Kontrol Rendang 4x 0,109 0,101 0,106 0,103 0,106 0,100 0,104 0,102 0,106 0,105 0,106 0,103 0,109 0,112 0,116 0,113 0,116 0,113
123
Lampiran 9. Contoh cara perhitungan % hemolisis *sampel 11 Nov 06, pengenceran 1x jam ke-5
Diketahui: Absorbansi sampel = 0,137 Absorbansi Kontrol (-) = 0,115
% Hemolisis =
=
124
Lampiran 10. Tabel data hasil pembacaan absorbansi pengukuran kapasitas antioksidan (metode DPPH) Sampel Absorbansi 1x 2x 4x 11 Nov 06 1,190 1,035 1,020 1,193 1,030 1,024 DIPA 14 Jun 07 1,050 1,030 1,020 1,051 1,033 1,022 No label 14 Jun 07 1,060 1,055 1,045 1,060 1,057 1,040 Kontrol Rendang 0,995 1,025 1,045 0,996 1,025 1,044
125
Lampiran 11. Contoh perhitungan untuk mendapatkan % kapasitas antioksidan tersisa
*sampel 11 Nov 06 pengenceran 1x ulangan 1
Diketahui: Abs sampel: 1,190 Abs kontrol (-): 1,350 maka
% Kapasitas Antioksidan tersisa =
=
= 11,85%
Kapasitas ini setara dengan kapasitas antioksidan vitamin C (x) sebesar: y = 1,3227 – 0,0011x
1,190 = 1,3227 – 0,0011x x = 120,64 ppm
126
Lampiran 12. Tabel data hasil pembacaan absorbansi pengukuran kadar Malonaldehida Sampel Absorbansi 1x 2x 4x 11 Nov 06 0,458 0,250 0,170 0,432 0,238 0,172 DIPA 14 Jun 07 0,464 0,283 0,166 0,370 0,207 0,110 No label 14 Jun 07 0,462 0,270 0,180 0,538 0,369 0,213 Kontrol Rendang 0,542 0,340 0,230 0,562 0,310 0,225
127
Lampiran 13. Contoh perhitungan untuk mendapatkan kadar malonaldehida *sampel 11 Nov 06 pengenceran 1x ulangan 1
Diketahui : Abs sampel : 0,458 (y)
Dengan memasukkan nilai y = 0,458 ke persamaan y= 0,0025x + 0,1172 maka
y = 0,0025x + 0,1172 0,458 = 0,0025x + 0,1172 0,3408 = 0,0025x
128
Lampiran 14. Hasil analisis statistik uji proliferasi limfosit pada selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors N Sampel 11 Nov 06 3 DIPA14Jun 3 Kontrol_R 3 Ktrl Std 3 LPS 3 NL14Jun 3 PWM 3 Ulangan 1 7 2 7 3 7
Tests of Between-Subjects Effects Dependent Variable: Absorbansi
Source
Type III Sum
of Squares Df Mean Square F Sig.
Model 44,445(a) 9 4,938 43,894 ,000
Sampel 2,754 6 ,459 4,080 ,018
Ulangan ,660 2 ,330 2,932 ,092
Error 1,350 12 ,113
Total 45,795 21
a R Squared = ,971 (Adjusted R Squared = ,948)
Post Hoc Tests
129 Multi ple Comparisons
Dependent Variable: Absorbansi Tukey HSD -,28967 ,273871 ,929 -1,24818 ,66885 ,19633 ,273871 ,989 -,76218 1,15485 ,45200 ,273871 ,657 -,50652 1,41052 ,39900 ,273871 ,763 -,55952 1,35752 -,62567 ,273871 ,324 -1,58418 ,33285 ,24733 ,273871 ,965 -,71118 1,20585 ,28967 ,273871 ,929 -,66885 1,24818 ,48600 ,273871 ,586 -,47252 1,44452 ,74167 ,273871 ,177 -,21685 1,70018 ,68867 ,273871 ,235 -,26985 1,64718 -,33600 ,273871 ,871 -1,29452 ,62252 ,53700 ,273871 ,482 -,42152 1,49552 -,19633 ,273871 ,989 -1,15485 ,76218 -,48600 ,273871 ,586 -1,44452 ,47252 ,25567 ,273871 ,959 -,70285 1,21418 ,20267 ,273871 ,987 -,75585 1,16118 -,82200 ,273871 ,112 -1,78052 ,13652 ,05100 ,273871 1,000 -,90752 1,00952 -,45200 ,273871 ,657 -1,41052 ,50652 -,74167 ,273871 ,177 -1,70018 ,21685 -,25567 ,273871 ,959 -1,21418 ,70285 -,05300 ,273871 1,000 -1,01152 ,90552 -1,07767* ,273871 ,024 -2,03618 -,11915 -,20467 ,273871 ,986 -1,16318 ,75385 -,39900 ,273871 ,763 -1,35752 ,55952 -,68867 ,273871 ,235 -1,64718 ,26985 -,20267 ,273871 ,987 -1,16118 ,75585 ,05300 ,273871 1,000 -,90552 1,01152 -1,02467* ,273871 ,033 -1,98318 -,06615 -,15167 ,273871 ,997 -1,11018 ,80685 ,62567 ,273871 ,324 -,33285 1,58418 ,33600 ,273871 ,871 -,62252 1,29452 ,82200 ,273871 ,112 -,13652 1,78052 1,07767* ,273871 ,024 ,11915 2,03618 1,02467* ,273871 ,033 ,06615 1,98318 ,87300 ,273871 ,083 -,08552 1,83152 -,24733 ,273871 ,965 -1,20585 ,71118 -,53700 ,273871 ,482 -1,49552 ,42152 -,05100 ,273871 1,000 -1,00952 ,90752 ,20467 ,273871 ,986 -,75385 1,16318 ,15167 ,273871 ,997 -,80685 1,11018 -,87300 ,273871 ,083 -1,83152 ,08552 (J) Sampel DIPA14Jun Kontrol_R Ktrl Std LPS NL14Jun PWM 11 Nov 06 Kontrol_R Ktrl Std LPS NL14Jun PWM 11 Nov 06 DIPA14Jun Ktrl Std LPS NL14Jun PWM 11 Nov 06 DIPA14Jun Kontrol_R LPS NL14Jun PWM 11 Nov 06 DIPA14Jun Kontrol_R Ktrl Std NL14Jun PWM 11 Nov 06 DIPA14Jun Kontrol_R Ktrl Std LPS PWM 11 Nov 06 DIPA14Jun Kontrol_R Ktrl Std LPS NL14Jun (I) Sampel 11 Nov 06 DIPA14Jun Kontrol_R Ktrl Std LPS NL14Jun PWM Mean Dif f erence
(I-J) Std. Error Sig. Lower Bound Upper Bound
95% Conf idence Interv al
Based on observ ed means.
The mean dif f erence is signif icant at the ,05 lev el. *.
130
Homogeneous Subsets
Absorbansi Tukey HSD Sampel N Subset 1 2 Ktrl Std 3 1,00000 LPS 3 1,05300 PWM 3 1,20467 1,20467 Kontrol_R 3 1,25567 1,25567 11 Nov 06 3 1,45200 1,45200 DIPA14Jun 3 1,74167 1,74167 NL14Jun 3 2,07767 Sig. ,177 ,083Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares
The error term is Mean Square(Error) = ,113. a Uses Harmonic Mean Sample Size = 3,000. b Alpha = ,05.
131
Lampiran 15. Hasil analisis statistik uji proliferasi limfosit semua sampel pada selang kepercayaan 99%
Univariate Analysis of Variance
Between-Subjects Factors N Sampel 11 Nov 06 3 DIPA14Jun 3 Kontrol_R 3 Ktrl Std 3 LPS 3 NL14Jun 3 PWM 3 Ulangan 1 7 2 7 3 7
Tests of Between-Subjects Effects Dependent Variable: Absorbansi
Source
Type III Sum
of Squares df Mean Square F Sig.
Model 44,445(a) 9 4,938 43,894 ,000
Sampel 2,754 6 ,459 4,080 ,018
Ulangan ,660 2 ,330 2,932 ,092
Error 1,350 12 ,113
Total 45,795 21
132
Lampiran 16. Hasil analisis statistik uji proliferasi limfosit sampel 11 Nov 06 pada berbagai pengenceran pada selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors N Sampel 11Nov06_1 3 11Nov06_2 3 11Nov06_4 3 Ulangan 1 3 2 3 3 3
Tests of Between-Subjects Effects Dependent Variable: IndeksStimulasi
Source
Type III Sum
of Squares df Mean Square F Sig.
Model 21,659(a) 5 4,332 85,677 ,000
Sampel ,011 2 ,005 ,107 ,901
Ulangan 1,819 2 ,909 17,986 ,010
Error ,202 4 ,051
Total 21,861 9
133
Lampiran 17. Hasil analisis statistik uji proliferasi limfosit sampel DIPA 14 Juni 07 pada berbagai pengenceran pada selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors N Sampel DIPA_1 3 DIPA_2 3 DIPA_4 3 Ulangan 1 3 2 3 3 3
Tests of Between-Subjects Effects Dependent Variable: IndeksStimulasi
Source
Type III Sum
of Squares df Mean Square F Sig.
Model 19,208(a) 5 3,842 32,477 ,002
Sampel ,447 2 ,224 1,890 ,264
Ulangan ,038 2 ,019 ,159 ,858
Error ,473 4 ,118
Total 19,681 9
134
Lampiran 18. Hasil analisis statistik uji proliferasi limfosit sampel No Label 14 Juni 07 pada berbagai pengenceran pada selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors N Sampel NL_1 3 NL_2 3 NL_3 3 Ulangan 1 3 2 3 3 3
Tests of Between-Subjects Effects Dependent Variable: IndeksStimulasi
Source
Type III Sum
of Squares df Mean Square F Sig.
Model 20,185(a) 5 4,037 47,989 ,001
Sampel 2,041 2 1,020 12,131 ,020
Ulangan ,183 2 ,092 1,089 ,419
Error ,336 4 ,084
Total 20,521 9
a R Squared = ,984 (Adjusted R Squared = ,963)
Post Hoc Tests
Sampel
Multi ple Comparisons Dependent Variable: IndeksStimulasi
Tukey HSD 1,08967* ,236815 ,022 ,24566 1,93367 ,90533* ,236815 ,040 ,06133 1,74934 -1,08967* ,236815 ,022 -1,93367 -,24566 -,18433 ,236815 ,735 -1,02834 ,65967 -,90533* ,236815 ,040 -1,74934 -,06133 ,18433 ,236815 ,735 -,65967 1,02834 (J) Sampel NL_2 NL_3 NL_1 NL_3 NL_1 NL_2 (I) Sampel NL_1 NL_2 NL_3 Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound
95% Conf idence Interv al
Based on observ ed means.
The mean dif f erence is signif icant at t he ,05 lev el. *.
135
Homogeneous Subsets
IndeksStimulasi Tukey HSD Sampel N Subset 1 2 NL_2 3 ,98800 NL_3 3 1,17233 NL_1 3 2,07767 Sig. ,735 1,000Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares
The error term is Mean Square(Error) = ,084. a Uses Harmonic Mean Sample Size = 3,000. b Alpha = ,05.
136
Lampiran 19. Hasil analisis statistik uji proliferasi limfosit sampel No Label 14 Juni 07 pada berbagai pengenceran pada selang kepercayaan 99%
Univariate Analysis of Variance
Between-Subjects Factors N Sampel NL_1 3 NL_2 3 NL_3 3 Ulangan 1 3 2 3 3 3
Tests of Between-Subjects Effects Dependent Variable: IndeksStimulasi
Source
Type III Sum
of Squares df Mean Square F Sig.
Model 20,185(a) 5 4,037 47,989 ,001
Sampel 2,041 2 1,020 12,131 ,020
Ulangan ,183 2 ,092 1,089 ,419
Error ,336 4 ,084
Total 20,521 9
137
Lampiran 20. Hasil analisis statistik uji proliferasi limfosit sampel Kontrol Rendang pada berbagai pengenceran pada selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors N Sampel K_1 3 K_2 3 K_3 3 Ulangan 1 3 2 3 3 3
Tests of Between-Subjects Effects Dependent Variable: IndeksStimulasi
Source
Type III Sum
of Squares df Mean Square F Sig.
Model 11,697(a) 5 2,339 289,202 ,000
Sampel ,085 2 ,042 5,247 ,076
Ulangan ,152 2 ,076 9,369 ,031
Error ,032 4 ,008
Total 11,729 9
138
Lampiran 21. Hasil analisis statistik uji hemolisis eritrosit sampel 11 Nov 06 pada jam ke-5 pada selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors N Sampel 1,00 3 2,00 3 3,00 3 Ulangan 1,00 3 2,00 3 3,00 3
Tests of Between-Subjects Effects Dependent Variable: Absorbansi
,171a 5 ,034 32,179 ,003 ,001 2 ,000 ,304 ,754 ,006 2 ,003 3,048 ,157 ,004 4 ,001 ,175 9 Source Model Sampel Ulangan Error Total Ty pe II I Sum
of Squares df Mean Square F Sig.
R Squared = , 976 (Adjusted R Squared = ,945) a.
139
Lampiran 22. Hasil analisis statistik uji hemolisis eritrosit sampel DIPA 14 Juni 2007 pada jam ke-5 pada selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors 3 3 3 3 3 3 1,00 2,00 3,00 Sampel 1,00 2,00 3,00 Ulangan N
Tests of Between-Subjects Effects Dependent Variable: Absorbansi
,134a 5 ,027 290,417 ,000 ,001 2 ,000 4,336 ,100 5,00E-005 2 2,50E-005 ,271 ,776 ,000 4 9,23E-005 ,134 9 Source Model Sampel Ulangan Error Total Ty pe II I Sum
of Squares df Mean Square F Sig.
R Squared = , 997 (Adjusted R Squared = ,994) a.
140
Lampiran 23. Hasil analisis statistik uji hemolisis eritrosit sampel No Label 14 Juni 2007 pada jam ke-5 pada selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors 3 3 3 3 4 4 4 1,00 2,00 3,00 4,00 Sampel 1,00 2,00 3,00 Ulangan N
Tests of Between-Subjects Effects Dependent Variable: Absorbansi
,198a 6 ,033 186,486 ,000 ,002 3 ,001 3,823 ,076 ,001 2 ,000 1,538 ,289 ,001 6 ,000 ,199 12 Source Model Sampel Ulangan Error Total Ty pe II I Sum
of Squares df Mean Square F Sig.
R Squared = , 995 (Adjusted R Squared = ,989) a.
141
Lampiran 24. Hasil analisis statistik uji hemolisis eritrosit sampel kontrol pada jam ke-5 pada selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors 3 3 3 3 3 3 1,00 2,00 3,00 Sampel 1,00 2,00 3,00 Ulangan N
Tests of Between-Subjects Effects Dependent Variable: Absorbansi
,162a 5 ,032 134,475 ,000 ,003 2 ,002 6,895 ,051 ,000 2 ,000 ,769 ,522 ,001 4 ,000 ,163 9 Source Model Sampel Ulangan Error Total Ty pe II I Sum
of Squares df Mean Square F Sig.
R Squared = , 994 (Adjusted R Squared = ,987) a.
142
Lampiran 25. Hasil analisis statistik persen hemolisis eritrosit semua sampel pada jam ke-5 pada selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors 3 3 2 3 3 5 5 4 1,00 2,00 3,00 4,00 5,00 Sampel 1,00 2,00 3,00 Ulangan N
Tests of Between-Subjects Effects Dependent Variable: PersenHemo
157585,422a 7 22512,203 120,325 ,000 726,438 4 181,610 ,971 ,480 2295,430 2 1147,715 6,134 ,029 1309,665 7 187,095 158895,088 14 Source Model Sampel Ulangan Error Total Ty pe I II Sum
of Squares df Mean Square F Sig.
R Squared = , 992 (Adjusted R Squared = ,984) a.
143
Lampiran 26. Hasil analisis statistik uji kapasitas antioksidan DPPH semua sampel pada pengenceran 1x dan selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors 2 2 2 2 4 4 1 2 3 4 Sampel 1 2 Ulangan N
Tests of Between-Subjects Effects Dependent Variable: KapAnt iox
3561,977a 5 712,395 167786,9 ,000 227,374 3 75,791 17850,719 ,000 ,017 1 ,017 4,030 ,138 ,013 3 ,004 3561,990 8 Source Model Sampel Ulangan Error Total Ty pe I II Sum
of Squares df Mean Square F Sig.
R Squared = 1, 000 (Adjusted R Squared = 1,000) a.
Post Hoc Tests
Sampel
144 Multi ple Comparisons
Dependent Variable: KapAntiox Tukey HSD 14,5200* ,06516 ,000 14,2056 14,8344 4,0750* ,06516 ,000 3,7606 4,3894 4,7800* ,06516 ,000 4,4656 5,0944 -14,5200* ,06516 ,000 -14,8344 -14,2056 -10,4450* ,06516 ,000 -10,7594 -10,1306 -9,7400* ,06516 ,000 -10,0544 -9,4256 -4,0750* ,06516 ,000 -4,3894 -3,7606 10,4450* ,06516 ,000 10,1306 10,7594 ,7050* ,06516 ,005 ,3906 1,0194 -4,7800* ,06516 ,000 -5,0944 -4,4656 9,7400* ,06516 ,000 9,4256 10,0544 -,7050* ,06516 ,005 -1,0194 -,3906 (J) Sampel 2,00 3,00 4,00 1,00 3,00 4,00 1,00 2,00 4,00 1,00 2,00 3,00 (I) Sampel 1,00 2,00 3,00 4,00 Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound
95% Conf idence Interv al
Based on observ ed means.
The mean dif f erence is signif icant at t he ,05 lev el. *.
Homogeneous Subsets
KapAntiox Tukey HSDa,b 2 11,7400 2 21,4800 2 22,1850 2 26,2600 1,000 1,000 1,000 1,000 Sampel 2,00 4,00 3,00 1,00 Sig. N 1 2 3 4 SubsetMeans f or groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = ,004. Uses Harmonic Mean Sample Size = 2,000. a.
Alpha = ,05. b.
145
Lampiran 27. Hasil analisis statistik uji kapasitas antioksidan DPPH semua sampel pada pengenceran 1x dan selang kepercayaan 99%
Univariate Analysis of Variance
Between-Subjects Factors 2 2 2 2 4 4 1,00 2,00 3,00 4,00 Sampel 1,00 2,00 Ulangan N
Tests of Between-Subjects Effects Dependent Variable: KapAnt iox
3561,977a 5 712,395 167786,9 ,000 227,374 3 75,791 17850,719 ,000 ,017 1 ,017 4,030 ,138 ,013 3 ,004 3561,990 8 Source Model Sampel Ulangan Error Total Ty pe I II Sum
of Squares df Mean Square F Sig.
R Squared = 1, 000 (Adjusted R Squared = 1,000) a.
Post Hoc Tests
146 Multi ple Comparisons
Dependent Variable: KapAntiox Tukey HSD 14,5200* ,06516 ,000 13,9593 15,0807 4,0750* ,06516 ,000 3,5143 4,6357 4,7800* ,06516 ,000 4,2193 5,3407 -14,5200* ,06516 ,000 -15,0807 -13,9593 -10,4450* ,06516 ,000 -11,0057 -9,8843 -9,7400* ,06516 ,000 -10,3007 -9,1793 -4,0750* ,06516 ,000 -4,6357 -3,5143 10,4450* ,06516 ,000 9,8843 11,0057 ,7050* ,06516 ,005 ,1443 1,2657 -4,7800* ,06516 ,000 -5,3407 -4,2193 9,7400* ,06516 ,000 9,1793 10,3007 -,7050* ,06516 ,005 -1,2657 -,1443 (J) Sampel 2,00 3,00 4,00 1,00 3,00 4,00 1,00 2,00 4,00 1,00 2,00 3,00 (I) Sampel 1,00 2,00 3,00 4,00 Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound
99% Conf idence Interv al
Based on observ ed means.
The mean dif f erence is signif icant at t he ,01 lev el. *.
Homogeneous Subsets
KapAntiox Tukey HSDa,b 2 11,7400 2 21,4800 2 22,1850 2 26,2600 1,000 1,000 1,000 1,000 Sampel 2,00 4,00 3,00 1,00 Sig. N 1 2 3 4 SubsetMeans f or groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = ,004. Uses Harmonic Mean Sample Size = 2,000. a.
Alpha = ,01. b.
147
Lampiran 28. Hasil analisis statistik pengukuran kadar MDA semua sampel pada pengenceran 1x dan selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors 4 4 2 2 2 2 1 2 Ulangan 11Nov 06 DIPA Kontrol NoLabel Sampel N
Tests of Between-Subjects Effects Dependent Variable: kadarMDA
170545,843a 5 34109,169 82,289 ,002 11,520 1 11,520 ,028 ,878 3446,080 3 1148,693 2,771 ,212 1243,520 3 414,507 171789,363 8 Source Model Ulangan Sampel Error Total Ty pe I II Sum
of Squares df Mean Square F Sig.
R Squared = , 993 (Adjusted R Squared = ,981) a.
148
Lampiran 29. Hasil analisis statistik pengukuran kadar MDA sampel 11 Nov 2006 pada berbagai pengenceran dan selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors 3 3 2 2 2 1 2 Ulangan 11Nov _1 11Nov _2 11Nov _4 Sampel N
Tests of Between-Subjects Effects Dependent Variable: kadarMDA
40490,726a 4 10122,682 645,579 ,002 34,560 1 34,560 2,204 ,276 12885,973 2 6442,987 410,905 ,002 31,360 2 15,680 40522,086 6 Source Model Ulangan Sampel Error Total Ty pe I II Sum
of Squares df Mean Square F Sig.
R Squared = , 999 (Adjusted R Squared = ,998) a.
Post Hoc Tests
149 Multi ple Comparisons
Dependent Variable: kadarMDA Tukey HSD 80,40000* 3,959798 ,004 57,07380 103,72620 109,60000* 3,959798 ,002 86,27380 132,92620 -80,40000* 3,959798 ,004 -103,72620 -57,07380 29,20000* 3,959798 ,032 5,87380 52,52620 -109,60000* 3,959798 ,002 -132,92620 -86,27380 -29,20000* 3,959798 ,032 -52,52620 -5,87380 (J) Sampel 11Nov _2 11Nov _4 11Nov _1 11Nov _4 11Nov _1 11Nov _2 (I) Sampel 11Nov _1 11Nov _2 11Nov _4 Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound
95% Conf idence Interv al
Based on observ ed means.
The mean dif f erence is signif icant at t he ,05 lev el. *.
Homogeneous Subsets
kadarMDA Tukey HSDa,b 2 21,52000 2 50,72000 2 131,12000 1,000 1,000 1,000 Sampel 11Nov _4 11Nov _2 11Nov _1 Sig. N 1 2 3 SubsetMeans f or groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 15,680. Uses Harmonic Mean Sample Size = 2,000. a.
Alpha = , 05. b.
150
Lampiran 30. Hasil analisis statistik pengukuran kadar MDA sampel 11 Nov 2006 pada berbagai pengenceran dan selang kepercayaan 99%
Univariate Analysis of Variance
Between-Subjects Factors 2 2 2 3 3 11Nov _1 11Nov _2 11Nov _4 Sampel 1 2 Ulangan N
Tests of Between-Subjects Effects Dependent Variable: kadarMDA
40490,726a 4 10122,682 645,579 ,002 12885,973 2 6442,987 410,905 ,002 34,560 1 34,560 2,204 ,276 31,360 2 15,680 40522,086 6 Source Model Sampel Ulangan Error Total Ty pe I II Sum
of Squares df Mean Square F Sig.
R Squared = , 999 (Adjusted R Squared = ,998) a.
Post Hoc Tests
Sampel
151 Multi ple Comparisons
Dependent Variable: kadarMDA Tukey HSD 80,40000* 3,959798 ,004 27,14642 133,65358 109,60000* 3,959798 ,002 56,34642 162,85358 -80,40000* 3,959798 ,004 -133,65358 -27,14642 29,20000 3,959798 ,032 -24,05358 82,45358 -109,60000* 3,959798 ,002 -162,85358 -56,34642 -29,20000 3,959798 ,032 -82,45358 24,05358 (J) Sampel 11Nov _2 11Nov _4 11Nov _1 11Nov _4 11Nov _1 11Nov _2 (I) Sampel 11Nov _1 11Nov _2 11Nov _4 Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound
99% Conf idence Interv al
Based on observ ed means.
The mean dif f erence is signif icant at t he ,01 lev el. *.
Homogeneous Subsets
kadarMDA Tukey HSDa,b 2 21,52000 2 50,72000 2 131,12000 ,032 1,000 Sampel 11Nov _4 11Nov _2 11Nov _1 Sig. N 1 2 SubsetMeans f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 15,680. Uses Harmonic Mean Sample Size = 2,000. a.
Alpha = ,01. b.
152
Lampiran 31. Hasil analisis statistik pengukuran kadar MDA sampel DIPA 14 Juni 2007 pada berbagai pengenceran dan selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors 2 2 2 3 3 DIPA_1 DIPA_2 DIPA_4 Sampel 1 2 Ulangan N
Tests of Between-Subjects Effects Dependent Variable: kadarMDA
35455,262a 4 8863,815 213,972 ,005 12386,210 2 6193,105 149,501 ,007 1276,625 1 1276,625 30,818 ,031 82,850 2 41,425 35538,112 6 Source Model Sampel Ulangan Error Total Ty pe II I Sum
of Squares df Mean Square F Sig.
R Squared = , 998 (Adjusted R Squared = ,993) a.
Post Hoc Tests
153 Multi ple Comparisons
Dependent Variable: kadarMDA Tukey HSD 68,80000* 6,436231 ,016 30,88574 106,71426 110,16000* 6,436231 ,006 72,24574 148,07426 -68,80000* 6,436231 ,016 -106,71426 -30,88574 41,36000* 6,436231 ,042 3,44574 79,27426 -110,16000* 6,436231 ,006 -148,07426 -72,24574 -41,36000* 6,436231 ,042 -79,27426 -3,44574 (J) Sampel DIPA_2 DIPA_4 DIPA_1 DIPA_4 DIPA_1 DIPA_2 (I) Sampel DIPA_1 DIPA_2 DIPA_4 Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound
95% Conf idence Interv al
Based on observ ed means.
The mean dif f erence is signif icant at t he ,05 lev el. *.
Homogeneous Subsets
kadarMDA Tukey HSDa,b 2 9,76000 2 51,12000 2 119,92000 1,000 1,000 1,000 Sampel DIPA_4 DIPA_2 DIPA_1 Sig. N 1 2 3 SubsetMeans f or groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 41,425. Uses Harmonic Mean Sample Size = 2,000. a.
Alpha = ,05. b.
154
Lampiran 32. Hasil analisis statistik pengukuran kadar MDA sampel DIPA 14 Juni 2007 pada berbagai pengenceran dan selang kepercayaan 99%
Univariate Analysis of Variance
Between-Subjects Factors 3 3 2 2 2 1 2 Ulangan DIPA_1 DIPA_2 DIPA_4 Sampel N
Tests of Between-Subjects Effects Dependent Variable: kadarMDA
35455,262a 4 8863,815 213,972 ,005 1276,625 1 1276,625 30,818 ,031 12386,210 2 6193,105 149,501 ,007 82,850 2 41,425 35538,112 6 Source Model Ulangan Sampel Error Total Ty pe I II Sum
of Squares df Mean Square F Sig.
R Squared = , 998 (Adjusted R Squared = ,993) a.
Post Hoc Tests
Sampel
Multi ple Comparisons Dependent Variable: kadarMDA
Tukey HSD 68,80000 6,436231 ,016 -17,75804 155,35804 110,16000* 6,436231 ,006 23,60196 196,71804 -68,80000 6,436231 ,016 -155,35804 17,75804 41,36000 6,436231 ,042 -45,19804 127,91804 -110,16000* 6,436231 ,006 -196,71804 -23,60196 -41,36000 6,436231 ,042 -127,91804 45,19804 (J) Sampel DIPA_2 DIPA_4 DIPA_1 DIPA_4 DIPA_1 DIPA_2 (I) Sampel DIPA_1 DIPA_2 DIPA_4 Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound
99% Conf idence Interv al
Based on observ ed means.
The mean dif f erence is signif icant at t he ,01 lev el. *.
155
Homogeneous Subsets
kadarMDA Tukey HSDa,b 2 9,76000 2 51,12000 51,12000 2 119,92000 ,042 ,016 Sampel DIPA_4 DIPA_2 DIPA_1 Sig. N 1 2 SubsetMeans f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 41,425. Uses Harmonic Mean Sample Size = 2,000. a.
Alpha = ,01. b.
156
Lampiran 33. Hasil analisis statistik pengukuran kadar MDA sampel No Label 14 Juni 2007 pada berbagai pengenceran dan selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors 2 2 2 3 3 NLabel_1 NLabel_2 NLabel_4 Sampel 1 2 Ulangan N
Tests of Between-Subjects Effects Dependent Variable: kadarMDA
63153,585a 4 15788,396 175,843 ,006 14914,293 2 7457,147 83,054 ,012 1153,707 1 1153,707 12,849 ,070 179,573 2 89,787 63333,158 6 Source Model Sampel Ulangan Error Total Ty pe II I Sum
of Squares df Mean Square F Sig.
R Squared = , 997 (Adjusted R Squared = ,991) a.
Post Hoc Tests
157 Multi ple Comparisons
Dependent Variable: kadarMDA Tukey HSD 72,20000* 9,475583 ,031 16,38166 128,01834 121,40000* 9,475583 ,011 65,58166 177,21834 -72,20000* 9,475583 ,031 -128,01834 -16,38166 49,20000 9,475583 ,063 -6,61834 105,01834 -121,40000* 9,475583 ,011 -177,21834 -65,58166 -49,20000 9,475583 ,063 -105,01834 6,61834 (J) Sampel NLabel_2 NLabel_4 NLabel_1 NLabel_4 NLabel_1 NLabel_2 (I) Sampel NLabel_1 NLabel_2 NLabel_4 Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound
95% Conf idence Interv al
Based on observ ed means.
The mean dif f erence is signif icant at t he ,05 lev el. *.
Homogeneous Subsets
kadarMDA Tukey HSDa,b 2 31,72000 2 80,92000 2 153,12000 ,063 1,000 Sampel NLabel_4 NLabel_2 NLabel_1 Sig. N 1 2 SubsetMeans f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 89,787. Uses Harmonic Mean Sample Size = 2,000. a.
Alpha = ,05. b.
158
Lampiran 34. Hasil analisis statistik pengukuran kadar MDA sampel No Label 14 Juni 2007 pada berbagai pengenceran dan selang kepercayaan 99%
Univariate Analysis of Variance
Between-Subjects Factors 3 3 2 2 2 1 2 Ulangan NL_1 NL_2 NL_4 Sampel N
Tests of Between-Subjects Effects Dependent Variable: kadarMDA
63153,585a 4 15788,396 175,843 ,006 1153,707 1 1153,707 12,849 ,070 14914,293 2 7457,147 83,054 ,012 179,573 2 89,787 63333,158 6 Source Model Ulangan Sampel Error Total Ty pe I II Sum
of Squares df Mean Square F Sig.
R Squared = , 997 (Adjusted R Squared = ,991) a.
Post Hoc Tests
159 Multi ple Comparisons
Dependent Variable: kadarMDA Tukey HSD 72,20000 9,475583 ,031 -55,23295 199,63295 121,40000 9,475583 ,011 -6,03295 248,83295 -72,20000 9,475583 ,031 -199,63295 55,23295 49,20000 9,475583 ,063 -78,23295 176,63295 -121,40000 9,475583 ,011 -248,83295 6,03295 -49,20000 9,475583 ,063 -176,63295 78,23295 (J) Sampel NL_2 NL_4 NL_1 NL_4 NL_1 NL_2 (I) Sampel NL_1 NL_2 NL_4 Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound
99% Conf idence Interv al
Based on observ ed means.
Homogeneous Subsets
kadarMDA Tukey HSDa,b 2 31,72000 2 80,92000 2 153,12000 ,011 Sampel NL_4 NL_2 NL_1 Sig. N 1 SubsetMeans f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 89,787. Uses Harmonic Mean Sample Size = 2,000. a.
Alpha = ,01. b.
160
Lampiran 35. Inform concern
INFORM CONCERN
PERNYATAAN KESEDIAAN MENJADI RESPONDEN PENELITIAN
Saya yang bertanda tangan di bawah ini:
Nama : Ari Try Purbayanto
Jenis kelamin : Laki-laki
Pekerjaan : Mahasiswa Ilmu dan Teknologi Pangan (ITP)
NRP : F24052702
Alamat : Jalan Raya Darmaga Gang Nurul Asman RT 01 RW Darmaga Bogor
Telepon : 085697486586
Menyatakan dalam keadaan sehat dan bersedia atau tidak berkeberatan untuk dilakukan pengambilan darah untuk keperluan penelitian yang berjudul “Kapasitas Antioksidan, Proliferasi Limfosit dan Hemolisis Eritrosit Manusia Ekstrak Rendang Iradiasi Dosis Tinggi.” Penelitian ini dilakukan dalam rangka penulisan skripsi oleh Kamalita Pertiwi/ NRP F24052300.
Pengambilan darah dilakukan di Klinik Farfa Darmaga- Bogor pada bulan Februari 2008 - April 2009.
Demikian keterangan ini dibuat untuk digunakan seperlunya.
Bogor, Februari 2008 Petugas Pengambil Darah Responden