Lampiran A. Data Rataan Jumlah Spermatozoa Mencit
Rataan jumlah spermatozoa mencit (Mus musculus) dengan pemberian vitamin E setelah mendapat kombinasi ekstrak air biji pepaya (Carica papaya L.) dan testosteron undekanoat (TU) (juta/ml, X±SD)
Lama Pemberian (minggu) Kontrol (juta/mL) Perlakuan (juta/mL)
T0 66.13±38.10 61.53±16.86 T1 39.83±7.26 133.27±49.33 T2 84.93±15.68 63.13±26.50 T3 104.40±13.41 11.47±5.57 T4 117.27±23.93 3.50±3.02 T5 65.23±23.83 27.07±23.50 T6 72.13±22.87 72.13±21.09
Hasil Uji Statistik SPSS
Mann Whitney Tests of Normality .108 35 .200* .961 35 .245 .203 35 .001 .845 35 .000 Kelom pok Kontrol Perlakuan JlhSperm
Statisti c df Sig. Statisti c df Sig.
Kolmogorov-Sm irnova Shapiro-Wi lk
This is a lower bound of the true s igni ficance. *.
Lil liefors Si gnifi cance Correction a.
Test of Homogeneity of Variance
3.387 1 68 .070 2.566 1 68 .114 2.566 1 54.820 .115 2.980 1 68 .089 Based on Mean Based on Median Based on Median and with adjusted df
Based on trimm ed m ean JlhSperm Levene Statisti c df1 df2 Sig. Ranks 35 44.36 1552.50 35 26.64 932.50 70 Kelompok Kontrol Perlakuan Total JlhSperm
Friedman test Wilcoxon Test Test Statisticsa 302.500 932.500 -3.641 .000 Mann-Whitney U Wi lcoxon W Z
As ymp. Sig. (2-tailed)
JlhSperm
Groupi ng Variable: Kelom pok a. Ranks 3.20 1.80 4.40 6.00 6.20 3.00 3.40 K0 K1 K2 K3 K4 K5 K6 Mean Rank Test Statisticsa 5 16.971 6 .009 N Chi-Square df As ymp. Sig. Friedman Test a. Test Statisticsc -1.214a -.674b -1.483b -2.023b -.405a -.405b -2.023b .225 .500 .138 .043 .686 .686 .043 Z
As ymp. Sig. (2-tailed)
K1 - K0 K2 - K0 K3 - K0 K4 - K0 K5 - K0 K6 - K0 K2 - K1
Based on positive ranks. a.
Based on negative ranks . b.
Wilcoxon Signed Ranks Test c.
Test Statisticsc
-2.023a -2.023a -1.753a -1.753a -2.023a -1.753a -2.023b
.043 .043 .080 .080 .043 .080 .043
Z
As ymp. Sig. (2-tailed)
K3 - K1 K4 - K1 K5 - K1 K6 - K1 K3 - K2 K4 - K2 K5 - K2
Based on negative ranks . a.
Based on positive ranks. b.
Wilcoxon Signed Ranks Test c.
Test Statisticsc
-.944a -.944b -2.023a -2.023a -1.753a -1.753a -.405b
.345 .345 .043 .043 .080 .080 .686
Z
As ymp. Sig. (2-tailed)
K6 - K2 K4 - K3 K5 - K3 K6 - K3 K5 - K4 K6 - K4 K6 - K5
Based on positive ranks. a.
Based on negative ranks . b.
Wilcoxon Signed Ranks Test c.
Friedman Test
Wilcoxon Test
T-TEST K0P0 (Transform square) Ranks 4.80 6.80 5.60 2.10 1.20 3.80 3.70 P0 P1 P2 P3 P4 P5 P6 Mean Rank Test Statisticsa 5 24.323 6 .000 N Chi-Square df As ymp. Sig. Friedm an Test a. Test Statisticsc -2.023a -.674a -2.023b -2.023b -1.753b -1.753b -1.753b .043 .500 .043 .043 .080 .080 .080 Z
As ymp. Sig. (2-tailed)
P1 - P0 P2 - P0 P3 - P0 P4 - P0 P5 - P0 P6 - P0 P2 - P1
Based on negative ranks . a.
Based on positive ranks. b.
Wilcoxon Signed Ranks Test c.
Test Statisticsb
-2.023a -2.023a -2.023a -2.023a -2.023a -2.023a -1.214a
.043 .043 .043 .043 .043 .043 .225
Z
As ymp. Sig. (2-tailed)
P3 - P1 P4 - P1 P5 - P1 P6 - P1 P3 - P2 P4 - P2 P5 - P2
Based on positive ranks. a.
Wilcoxon Signed Ranks Test b.
Test Statisticsc
-2.023a -1.753a -1.214b -1.826b -2.023b -2.023b -.405b
.043 .080 .225 .068 .043 .043 .686
Z
As ymp. Sig. (2-tailed)
P6 - P2 P4 - P3 P5 - P3 P6 - P3 P5 - P4 P6 - P4 P6 - P5
Based on positive ranks. a.
Based on negative ranks . b.
Wilcoxon Signed Ranks Test c.
Independent Samples Test
5.101 .054 .650 8 .534 1521.2660 2339.0580 -3872.61 6915.143 .650 5.568 .541 1521.2660 2339.0580 -4311.70 7354.235 Equal variances
as sumed Equal variances not ass umed K0P0
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
T-TEST K1P1 (Transform Log)
T-TEST K2P2
T-TEST K3P3
T-TEST K4P4 (Transform Square Root)
Independent Samples Test
4.492 .067 -4.190 8 .003 -93.43200 22.29961 -144.855 -42.00900 -4.190 4.173 .013 -93.43200 22.29961 -154.346 -32.51768 Equal variances
as sumed Equal variances not ass umed K1P1
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Independent Samples Test
.366 .562 1.583 8 .152 21.80000 13.76768 -9.94833 53.54833 1.583 6.494 .161 21.80000 13.76768 -11.27624 54.87624 Equal variances
as sumed Equal variances not ass umed K2P2
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Independent Samples Test
3.414 .102 14.308 8 .000 92.93400 6.49528 77.95585 107.91215 14.308 5.341 .000 92.93400 6.49528 76.55251 109.31549 Equal variances
as sumed Equal variances not ass umed K3P3
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Independent Samples Test
.009 .925 13.425 8 .000 9.17200 .68319 7.59656 10.74744 13.425 7.987 .000 9.17200 .68319 7.59610 10.74790 Equal variances assumed Equal variances not assumed K4P4 F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
T-TEST K5P5
T-TEST K6P6 (Transform Cube)
Independent Samples Test
.002 .963 2.550 8 .034 38.16600 14.96677 3.65257 72.67943 2.550 7.998 .034 38.16600 14.96677 3.65143 72.68057 Equal variances assumed Equal variances not assumed K5P5 F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Independent Samples Test
.230 .644 2.808 8 .023 39.07000 13.91313 6.98626 71.15374 2.808 7.948 .023 39.07000 13.91313 6.94997 71.19003 Equal variances assumed Equal variances not assumed K6P6 F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Lampiran B. Data Rataan Motilitas Spermatozoa Mencit
Rataan motilitas spermatozoa mencit (Mus musculus) dengan pemberian vitamin E setelah mendapat kombinasi ekstrak air biji pepaya (Carica papaya L.) dan testosteron undekanoat (TU) (%, X±SD)
Lama Pemberian (minggu) Kontrol (%) Perlakuan (%)
T0 68.83±28.11 71.67 ±15.17 T1 73.00±5.06 78.67±5.91 T2 84.83±4.27 81.83 ±3.75 T3 88.67±3.85 25.83±17.75 T4 87.00±2.09 11.17 ±13.18 T5 81.83 ±11.61 26.83 ±16.71 T6 84.67±6.96 52.00 ±20.86
Hasil Uji Statistik SPSS
Mann - Whitney Test
Tests of Normality .227 35 .000 .727 35 .000 .179 35 .006 .897 35 .003 Kelom pok Kontrol Perlakuan Motilitas
Statisti c df Sig. Statisti c df Sig.
Kolmogorov-Sm irnova Shapiro-Wi lk
Lil liefors Si gnifi cance Correction a.
Test of Homogeneity of Variance
40.970 1 68 .000 39.824 1 68 .000 39.824 1 66.360 .000 41.611 1 68 .000 Based on Mean Based on Median Based on Median and wi th adjusted df
Based on trimm ed m ean Motilitas Levene Statisti c df1 df2 Sig. Ranks 35 47.91 1677.00 35 23.09 808.00 70 Kelompok Kontrol Perlakuan Total Motilitas
N Mean Rank Sum of Ranks
Test Statisticsa 178.000 808.000 -5.106 .000 Mann-Whitney U Wilcoxon W Z
As ymp. Sig. (2-tailed)
Motilitas
Grouping Variable: Kelompok a.
Friedmann Test Friedmann Test Wilcoxon Test T-TEST K0P0 Ranks 4.00 1.60 3.40 5.60 4.50 4.30 4.60 K0 K1 K2 K3 K4 K5 k6 Mean Rank Test Statisticsa 5 10.086 6 .121 N Chi-Square df As ymp. Sig. Friedman Test a. Ranks 5.40 6.00 6.40 2.20 1.40 2.90 3.70 P0 P1 P2 P3 P4 P5 P6 Mean Rank Test Statisticsa 5 24.753 6 .000 N Chi-Square df As ymp. Sig. Friedman Test a. Test Statisticsc -.813a -1.483a -2.023b -2.023b -2.023b -1.483b -1.219a .416 .138 .043 .043 .043 .138 .223 Z
As ymp. Sig. (2-tailed)
P1 - P0 P2 - P0 P3 - P0 P4 - P0 P5 - P0 P6 - P0 P2 - P1
Based on negative ranks . a.
Based on positive ranks. b.
Wilcoxon Signed Ranks Test c.
Test Statisticsb
-2.023a -2.032a -2.023a -2.023a -2.023a -2.023a -2.023a
.043 .042 .043 .043 .043 .043 .043
Z
As ymp. Sig. (2-tailed)
P3 - P1 P4 - P1 P5 - P1 P6 - P1 P3 - P2 P4 - P2 P5 - P2
Based on positive ranks. a.
Wilcoxon Signed Ranks Test b.
Test Statisticsc
-2.023a -1.214a -.674b -1.753b -1.483b -2.023b -1.461b
.043 .225 .500 .080 .138 .043 .144
Z
As ymp. Sig. (2-tailed)
P6 - P2 P4 - P3 P5 - P3 P6 - P3 P5 - P4 P6 - P4 P6 - P5
Based on positive ranks. a.
Based on negative ranks . b.
Wilcoxon Signed Ranks Test c.
T-TEST K1P1
T-TEST K2P2
T-TEST K3P3 (Transform Square)
T-TEST K4P4 Ranks 5 5.90 29.50 5 5.10 25.50 10 Kelompok Kontrol Perlakuan Total K0P0
N Mean Rank Sum of Ranks
Test Statisticsb 10.500 25.500 -.419 .675 .690a Mann-Whitney U Wi lcoxon W Z
As ymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
K0P0
Not corrected for ties . a.
Groupi ng Variable: Kelom pok b.
Independent Samples Test
.332 .581 -1.630 8 .142 -5.67000 3.47843 -13.69126 2.35126 -1.630 7.814 .143 -5.67000 3.47843 -13.72470 2.38470 Equal variances
as sumed Equal variances not ass umed K1P1
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Independent Samples Test
.000 .999 1.182 8 .271 3.00000 2.53848 -2.85375 8.85375 1.182 7.870 .272 3.00000 2.53848 -2.87066 8.87066 Equal variances
as sumed Equal variances not ass umed K2P2
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Independent Samples Test
.179 .684 12.384 8 .000 6954.3080 561.55665 5659.356 8249.260
12.384 6.819 .000 6954.3080 561.55665 5619.278 8289.338 Equal variances
as sumed Equal variances not ass umed K3P3
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
T-TEST K5P5
T-TEST K6P6
Lampiran C. Data Rataan Viabilitas Spermatozoa Mencit Ranks 5 8.00 40.00 5 3.00 15.00 10 Kelompok Kontrol Perlakuan Total K4P4
N Mean Rank Sum of Ranks
Test Statisticsb .00 0 15 .000 -2.627 .00 9 .00 8a Ma nn-Whitn ey U Wi lcoxo n W Z
As ymp . Sig . (2-tailed ) Exact Sig. [2 *(1-taile d Sig .)]
K4 P4
No t correcte d fo r ties . a.
Groupi ng Variab le: Kelom pok b.
Independent Samples Test
.938 .361 6.042 8 .000 54.99800 9.10192 34.00893 75.98707 6.042 7.132 .000 54.99800 9.10192 33.55607 76.43993 Equal variances
as sumed Equal variances not ass umed K5P5
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Ranks 5 7.80 39.00 5 3.20 16.00 10 Kelompok Kontrol Perlakuan Total K6P6
N Mean Rank Sum of Ranks
Test Statisticsb 1.000 16.000 -2.410 .016 .016a Mann-Whitney U Wilcoxon W Z
As ymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
K6P6
Not corrected for ties . a.
Grouping Variable: Kelompok b.
Rataan viabilitas spermatozoa mencit (Mus musculus) dengan pemberian vitamin E setelah mendapat kombinasi ekstrak air biji pepaya (Carica papaya L.) dan testosteron undekanoat (%, X±SD)
Lama Pemberian (minggu) Kontrol (%) Perlakuan (%)
T0 70.00±26.36 56.17±0.95 T1 58.00±8.49 41.83±4.14 T2 70.17±18.77 30.67±7.18 T3 63.17±6.02 32.27±8.34 T4 67.67±9.10 24.33±3.79 T5 69.00 ±11.17 36.83 ±18.15 T6 59.40±6.11 47.67±20.16
Hasil Uji Statistik SPSS T-TEST K0P0
T-TEST K1P1
T-TEST K2P2
T-TEST K3P3
Independent Samples Test
45.546 .000 1.173 8 .275 13.83200 11.79563 -13.36876 41.03276 1.173 4.010 .306 13.83200 11.79563 -18.88438 46.54838 Equal variances
as sumed Equal variances not ass umed K0P0
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Independent Samples Test
5.334 .050 3.826 8 .005 16.16400 4.22449 6.42232 25.90568 3.826 5.801 .009 16.16400 4.22449 5.74067 26.58733 Equal variances
as sumed Equal variances not ass umed K1P1
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Independent Samples Test
2.149 .181 4.396 8 .002 39.50200 8.98530 18.78185 60.22215 4.396 5.146 .007 39.50200 8.98530 16.59983 62.40417 Equal variances
as sumed Equal variances not ass umed K2P2
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
T-TEST K4P4
T-TEST K5P5
T-TEST K6P6
Lampiran D. Data Rataan Morfologi Spermatozoa Mencit
Independent Samples Test
.202 .665 6.717 8 .000 30.90200 4.60083 20.29246 41.51154 6.717 7.277 .000 30.90200 4.60083 20.10601 41.69799 Equal variances
as sumed Equal variances not ass umed K3P3
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Independent Samples Test
1.749 .223 9.833 8 .000 43.33400 4.40696 33.17154 53.49646 9.833 5.349 .000 43.33400 4.40696 32.22442 54.44358 Equal variances
as sumed Equal variances not ass umed K4P4
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Independent Samples Test
2.215 .175 3.375 8 .010 32.16533 9.53019 10.18868 54.14199 3.375 6.651 .013 32.16533 9.53019 9.38824 54.94243 Equal variances
as sumed Equal variances not ass umed K5P5
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Independent Samples Test
6.104 .039 1.246 8 .248 11.73327 9.42021 -9.98977 33.45630 1.246 4.729 .271 11.73327 9.42021 -12.90636 36.37290 Equal variances
as sumed Equal variances not ass umed K6P6
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Rataan morfologi spermatozoa mencit (Mus musculus) dengan pemberian vitamin E setelah mendapat kombinasi ekstrak air biji pepaya (Carica papaya L.) dan testosteron undekanoat (TU) (%, X±SD)
Lama Pemberian (minggu) Kontrol (%) Perlakuan (%)
T0 93.53±1.27 83.17±1.37 T1 92.37±2.21 74.43±8.53 T2 93.17±1.81 70.90±7.83 T3 92.83±0.95 60.23±7.09 T4 92.83±0.46 67.33±10.13 T5 93.83±1.62 74.00±4.43 T6 92.50±1.18 73.83±2.01
Hasil Uji Statistik SPSS
Mann-Whitney TEST Tests of Normality .155 35 .033 .941 35 .060 .097 35 .200* .951 35 .120 KELOMPOK KONTROL PERLAKUAN MORFOLOGI
Statistic df Sig. Statistic df Sig.
Kolmogorov-Smirnova Shapiro-Wilk
This is a lower bound of the true s ignificance. *.
Lilliefors Significance Correction a.
Test of Homogeneity of Variance
45.037 1 68 .000 42.979 1 68 .000 42.979 1 36.281 .000 43.124 1 68 .000 Based on Mean Based on Median Based on Median and wi th adjusted df
Based on trimm ed m ean MORFOLOGI Levene Statisti c df1 df2 Sig. Ranks 35 53.00 1855.00 35 18.00 630.00 70 KELOMPOK KONTROL PERLAKUAN Total MORFOLOGI
Friedman Test
Friedman Test
Wilcoxon Test Test Statisticsa .000 630.000 -7.210 .000 Mann-Whitney U Wilcoxon W Z
As ymp. Sig. (2-tailed)
MORFOLOGI
Grouping Variable: KELOMPOK a. Ranks 4.60 3.50 4.30 3.90 3.80 4.80 3.10 K0 K1 K2 K3 K4 K5 K6 Mean Rank Test Statisticsa 5 2.510 6 .867 N Chi-Square df As ymp. Sig. Friedman Test a. Ranks 7.00 4.20 3.80 1.40 3.00 4.20 4.40 P0 P1 P2 P3 P4 P5 P6 Mean Rank Test Statisticsa 5 18.257 6 .006 N Chi-Square df As ymp. Sig. Friedman Test a. Test Statisticsb -2.023a -2.023a -2.023a -2.023a -2.023a -2.023a -.944a .043 .043 .043 .043 .043 .043 .345 Z
As ymp. Sig. (2-tailed)
P1 - P0 P2 - P0 P3 - P0 P4 - P0 P5 - P0 P6 - P0 P2 - P1
Based on positive ranks. a.
Wilcoxon Signed Ranks Test b.
Test Statisticsc
-1.761a -.813a -.135a -.405a -2.023a -.674a -.813b
.078 .416 .893 .686 .043 .500 .416
Z
As ymp. Sig. (2-tailed)
P3 - P1 P4 - P1 P5 - P1 P6 - P1 P3 - P2 P4 - P2 P5 - P2
Based on positive ranks. a.
Based on negative ranks . b.
Wilcoxon Signed Ranks Test c.
T-TEST K0P0 T-TEST K1P1 T-TEST K2P2 Test Statisticsc -.674a -1.214a -2.023a -2.023a -1.753a -1.625a .000b .500 .225 .043 .043 .080 .104 1.000 Z
As ymp. Sig. (2-tailed)
P6 - P2 P4 - P3 P5 - P3 P6 - P3 P5 - P4 P6 - P4 P6 - P5
Based on negative ranks . a.
The sum of negative ranks equals the sum of positive ranks. b.
Wilcoxon Signed Ranks Test c.
Independent Samples Test
.212 .658 12.435 8 .000 10.36600 .83359 8.44373 12.28827 12.435 7.951 .000 10.36600 .83359 8.44165 12.29035 Equal variances
as sumed Equal variances not ass umed K0P0
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Ranks 5 8.00 40.00 5 3.00 15.00 10 KELOMPOK KONTROL PERLAKUAN Total K1P1
N Mean Rank Sum of Ranks
Test Statisticsb .000 15.000 -2.611 .009 .008a Mann-Whitney U Wi lcoxon W Z
As ymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
K1P1
Not corrected for ties. a.
Groupi ng Variable: KELOMPOK b. Ranks 5 8.00 40.00 5 3.00 15.00 10 KELOMPOK KONTROL PERLAKUAN Total K2P2
N Mean Rank Sum of Ranks
Test Statisticsb .000 15.000 -2.619 .009 .008a Mann-Whitney U Wilcoxon W Z
As ymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
K2P2
Not corrected for ties. a.
Grouping Variable: KELOMPOK b.
T-TEST K3P3
T-TEST K4P4
T-TEST K5P5
T-TEST K6P6
Independent Samples Test
4.031 .080 10.190 8 .000 32.60200 3.19934 25.22431 39.97969 10.190 4.144 .000 32.60200 3.19934 23.83924 41.36476 Equal variances
as sumed Equal variances not ass umed K3P3
F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Ranks 5 8.00 40.00 5 3.00 15.00 10 KELOMPOK KONTROL PERLAKUAN Total K4P4
N Mean Rank Sum of Ranks
Test Statisticsb .000 15.000 -2.652 .008 .008a Mann-Whitney U Wi lcoxon W Z
As ymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
K4P4
Not corrected for ties. a.
Groupi ng Variable: KELOMPOK b. Ranks 5 8.00 40.00 5 3.00 15.00 10 KELOMPOK KONTROL PERLAKUAN Total K5P5
N Mean Rank Sum of Ranks
Test Statisticsb .000 15.000 -2.643 .008 .008a Mann-Whitney U Wilcoxon W Z
As ymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
K5P5
Not corrected for ties. a.
Grouping Variable: KELOMPOK b.
Independent Samples Test
2.616 .144 17.921 8 .000 18.66600 1.04155 16.26417 21.06783 17.921 6.472 .000 18.66600 1.04155 16.16182 21.17018 Equal variances assumed Equal variances not assumed K6P6 F Sig.
Levene's Test for Equality of Variances
t df Sig. (2-tailed) Mean Difference
Std. Error
Difference Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Lampiran E. Dokumentasi Penelitian
1. Foto Alat dan bahan
Gambar 1: Kamar Hitung pada Gambar2: Counter perbesaran 4x10
Gambar 5 : Jarum Gevage Gambar 6: Ekstrak air biji pepaya
2. Foto Kerja
Gambar 1: Pemberian Vitamin E Gambar 2 : Penyuntikan TU
Gambar 3 : Pengambilan kauda Gambar 4: Suspensi Spermatozoa epididimis
Gambar 5: Sperma dalam kamar Gambar 6: a). Sperma normal dan hitung b). Sperma abnormal Lampiran F. Cara Pembuatan Ekstrak Air Biji Pepaya
Diambil biji pepaya Dicuci biji
Dikeringkan dengan incubator pada suhu 50°C Dihaluskan dengan diblender dan diayak
Ditimbang bubuk halus biji pepaya
Dimasukkan kedalam bejana yang telah diisi air Direbus hingga mendidih
Disaring dengan kertas saring
Dipanaskan
Dilarutkan dengan akuabidestilata sesuai dengan kebutuhan penelitian
Pepaya (Carica papaya L.)
Bubuk halus biji pepaya
Air rebusan mendidih
Residu Hasil
Rendeimen
Lampiran G. Pembuatan Suspensi Vitamin E
Digunting permukaan kapsul Dituang kedalam gelas ukur Dilarutkan dengan Castrol oil Dihomogenkan
Kapsul Vitamin E
Lampiran H. Pengambilan Sekresi Cauda Epididimis
Didislokasi leher Dibedah
Diambil bagian cauda epididimis kanan dan kiri Diletakkan kedalam kaca arloji yang berisi 1 ml NaCl 0,9%
Dipotong sedikit dengan pisau dan jarum pentul dengan perlahan hingga epididimis keluar dan tersuspensi
Suspensi Sperma dari cauda epididimis Mencit (Mus musculus
Lampiran I. Penentuan Jumlah Spermatozoa (WHO,1998)
Dihomogenkan
Diteteskan pada kamar hitung Neubaeur Ditutup dengan kaca penutup
Diamati dibawah mikroskop cahaya dengan perbesaran 400x
Dihitung jumlah sperma pada kotak/ bidang A, B, C, D dan E
Dimasukkan kedalam rumus penentuan jumlah sperma /ml suspensi sekresi cauda epididimis
Jumlah Spermatozoa Suspensi Sperma dari
Lampiran J. Pengamatan Motilitas Spermatozoa (Partodihardjo, 1980)
Dihomogenkan
Diteteskan pada kamar hitung Neubaeur Ditutup dengan kaca penutup
Diamati dibawah mikroskop cahaya dengan perbesaran 400x
Dihitung jumlah spermatozoa yang bergerak dan tidak bergerak yang dinyatakan dalam % (Partodihardjo, 1980)
Motilitas Spermatozoa Suspensi Sperma dari
Lampiran K. Pengamatan Viabilitas Spermatozoa (WHO,1998)
Dihomogenkan
Diteteskan pada kamar hitung Neubaeur Ditutup dengan kaca penutup
Diteteskan pewarnaan giemsa disekitar pinggir kaca penutup
Diamati dibawah mikroskop cahaya dengan perbesaran 400x
Dihitung terhadap 100-200 spermatozoa dinyatakan dalam bentuk persen hidup yang didapat dari hasil bagi jumlah spermatozoa hidup dengan jumlah total spermatozoa hidup dan mati yang dikalikan dengan 100%
Viabilitas Spermatozoa
Suspensi Sperma dari cauda epididimis
Lampiran L. Pengamatan Morfologi Spermatozoa (WHO, 1998)
Dihomogenkan
Diteteskan pada kamar hitung Neubaeur Ditutup dengan kaca penutup
Diteteskan pewarnaan giemsa disekitar pinggir kaca penutup
Dilakukan pengamatan dibawah mikroskop dengan perbesaran 400x
Ditentukan pada kelainan bentuk primer dan sekunder. Berdasarkan hal ini dihitung spermatozoa yang normal dari 100-200 spermatozoa yang teramati.
Ditentukan persentase spermatozoa normal dengan cara membagi spermatozoa bentuk normal dengan jumlah total spermatozoa normal dan abnormal lalu dikalikan dengan 100%.
Morfologi Spermatozoa Suspensi Sperma dari
Lampiran M. Uji Skrinning Fitokimia Biji Pepaya Terhadap Alkaloid
Dikeringkan Dihaluskan
Diambil sebanyak 3 g dan dimasukkan kedalam erlemeyer yang berisi 100ml methanol
Dipanaskan hingga ¼ volume awal Disaring
Ditetesi pereaksi Ditetesi pereaksi Ditetesi pereaksi Ditetesi Meyer Wagner Bouchard dragendorf Diamati Diamati Diamati Diamati
Biji Pepaya (Carica papaya L.)
Ekstrak
Tabung I Tabung II Tabung III Tabung IV
Lampiran N. Uji Skrinning Fitokimia Biji Pepaya Terhadap Flavonoid
Dikeringkan Dihaluskan
Diambil sebanyak 3 g dan dimasukkan kedalam erlemeyer yang berisi 100ml methanol
Dipanaskan hingga ¼ volume awal Disaring
Ditetesi pereaksi Ditetesi pereaksi Ditetesi pereaksi Ditetesi FeCl3 MgHCl H2SO4 NaOH 10%
Diamati Diamati Diamati Diamati
Biji Pepaya (Carica papaya L.)
Ekstrak
Tabung I Tabung II Tabung III Tabung IV
Lampiran O. Uji Skrinning Fitokimia Biji Pepaya Terhadap Steroid
Dikeringkan Dihaluskan
Diambil sebanyak 3 g dan dimasukkan kedalam erlemeyer yang berisi 100ml n- heksan
Dipanaskan hingga ¼ volume awal Disaring
Ditetesi pereaksi Ditetesi reagent Salkowsky Ditetesi libe CeSO4 1% H2SO4 men-Bouchard
Diamati Diamati Diamati
Biji Pepaya (Carica papaya L.)
Ekstrak
Tabung I Tabung II Tabung III
Lampiran P. Uji Skrinning Fitokimia Biji Pepaya Terhadap Terpenoid
Dikeringkan Dihaluskan
Diambil sebanyak 3 g dan dimasukkan kedalam erlemeyer yang berisi 100ml kloroform
Dipanaskan hingga ¼ volume awal Disaring
Ditetesi pereaksi Ditetesi reagent Salkowsky Ditetesi Liber CeSO4 1% H2SO4 men- bauchard Diamati Diamati Diamati
Biji Pepaya (Carica papaya L.)
Ekstrak
Tabung I Tabung II Tabung III
Lampiran Q. Varietas-Varietas Pepaya (Carica papaya L.) di Indonesia.
1. Pepaya Medan
a. Pepaya Medan
Deskripsi Morfologi:
Kulit buah bewarna hijau, halus dan licin. Daging buah bewarna kuning kemerahan dan rasanya manis. Berat buah kurang lebih 1,5 kg.
b. Pepaya Bangkok
Deskripsi Morfologi:
Varietas pepaya Bangkok dikenal juga dengan nama pepaya Thailand. Kulit luar kasar dan tidak rata. Pepaya Bangkok berukuran lebih besar dan bentuknya lebih bulat dibandingkan pepaya Cibinong. Daging buah bewarna merah jingga dan berat buah kurang lebih 3,5 kg (Kalie, 1996).
3. Pepaya Cibinong
c. Pepaya Cibinong
Deskripsi Morfologi:
Bentuk buah panjang besar dan lancip pada bagian ujungnya. Tangkai buah panjang. Daging buah bewarna merah kekuningan, rasanya kurang manis. Berat varietas pepaya kurang lebih 2,5 kg/buah (Kalie, 1996).
4. Pepaya Solo
d. Pepaya Solo
Deskripsi Morfologi:
Ukuran buah kecil dan bentuknya mirip buah alpukat. Berat buah antara 0,4-0,1 kg/buah. Daging buah bewarna kuning, beraroma dan rasanya manis (Kalie, 1996).
e. Pepaya Burung
Deskripsi Morfologi:
Daging buah bewarna kuning dan berair banyak. Kulit buah bewarna hijau kekuning. Rasa asam manis (Kalie, 1996).
6. Pepaya Jinggo
f. Pepaya Jinggo
Deskripsi Morfologi:
Daging buah bewarna merah dan berair banyak. Kulit buah bewarna kuning. Berat buah kurang lebih 1,5 kg/buah (Kalie, 1996).
7. Pepaya California
g. Pepaya California
Deskripsi Morfologi:
Warnanya lebih mengkilap, bentuknya agak lonjong, daging buahnya tebal, bijinya lebih sedikit, dan rasanya manis (Kalie, 1996).
