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Lampiran 4. Prosedur pembuatan preparat
Judul : Korelasi Luas Area Wharton’s jelly dengan Luaran Berat Lahir Bayi pada Kehamilan Cukup Bulan
Tempat : Laboratorium Sentral RSUP Dr. Kariadi 1. Potong tali pusat sepanjang 2 cm dari ujung akhir
pemotongan, lalu masukkan kedalam container yang telah berisi Neutral Buffer Formalin dengan perbandingan 1:10. Beri Identitas dan bawa ke laboratorium Patologi Anatomi
2. Jaringan mentah dipotong menjadi 3 bagian (pilih bentuk potongan yang baik) lalu masukkan kedalam kaset. Lalu, masukkan kaset kedalam Neutral Buffer Formalin.
3. Pemasakan jaringan selama 17,5 jam :
4. Jaringan yang telah melalui proses pemasakan kemudian dibuat menjadi blok jaringan menggunakan alat histocenter. Kemudian blok parafin didinginkan kedalam freezer.
5. Setelah didinginkan, blok jaringan kemudian dpotong menggunakan mikrotom. Hasil pemotongan dimasukkan kedalam air hangat lalu ditempelkan pada kaca objek. Beri identitas
6. Preparat kemudian dipanaskan dalam oven 70% selama 10 menit untuk melelehkan sisa parafin. Kemudian dinginkan dan tata di rak.
7. Proses pewarnaan dengan alat Veristein dengan total waktu 2,5 jam :
- Xylol 1, 2, dan 3 masing-masing 10 menit - Etanol selama 3 menit
- Alkohol 96% selama 3 menit - Alkohol 80% selama 3 menit - Alkohol 70% selama 3 menit - Alkohol 50% selama 3 menit - Bilas dengan air mengalir - Hematoksilin selama 15 menit - HCl selama 1 menit
- Alkohol 50% selama 3 menit - Alkohol 70% selama 3 menit - Eosin selama 3 menit
- Alkohol 70% selama 3 menit - Alkohol 96% selama 3 menit - Etanol selama 3 menit
- Xylol 1, 2, dan 3 masing-masing selama 10 menit
Lampiran 5. Lembar pengumpulan data
3. Pendidikan terakhir : a. Tidak sekolah d. SMA/sederajat b. SD/sederajat e. Perguruan Tinggi c. SMP/sederajat
REKAM MEDIS
1. Interval kehamilan : …… bulan 2. Usia Kehamilan : …… minggu
9. Riwayat gangguan fungsi jantung selama kehamilan?
a. Ya b. Tidak
C. DATA BAYI
Jenis kelamin : Berat lahir :
D. HASIL PEMERIKSAAN HISTOFOTOMETRI
L.TP L.A1 L.A2 L.AT L.V L.PD L.WJ
mm2
Keterangan
L.TP : Luas area tali pusat
L.A1 : Luas area arteri umbilikal 1 L.A2 : Luas area arteri umbilikal 2
L.AT : Luas arteri umbilikal total (L.A1 + L.A2) L.V : Luas area vena umbilikal
L. PD : Luas area pembuluh darah (L.AT + L.V) L.WJ : Luas area Wharton’s jelly (L.TP – L.PD)
Semarang, 2016
Yang mengisi,
Lampiran 6. Hasil Pemeriksaan
No Usia
Ibu Paritas Pendidikan
Lampiran 6. Hasil Pemeriksaan (lanjutan)
No Usia
Ibu Paritas Pendidikan
Interval Kehamilan
Usia Kehamilan
Tinggi
Badan Hb
Jenis Kelamin Bayi
Berat
Lahir Bayi L.TP L.A1 L.A2 L.AT L.V L.PD L.WJ
28 31 1 Perguruan Tinggi 24 39 155 Laki-laki 3500 63.064 2.709 2.255 4.964 5.520 10.484 52.580
29 34 1 Perguruan Tinggi 24 38 158 10.4 Laki-laki 3900 49.217 1.438 2.497 3.935 3.120 7.055 42.162
30 36 2 SMA/sederajat 84 38 160 10.1 Laki-laki 3400 84.253 2.673 1.914 4.587 6.086 10.673 73.580
31 38 2 Perguruan Tinggi 108 39 160 12.2 Laki-laki 2900 59.083 3.954 3.058 7.012 5.165 12.177 46.906
34 32 2 SMA/sederajat 48 39 156 9.9 Laki-laki 2900 68.245 1.677 1.879 3.556 2.022 5.578 62.667
Lampiran 7. Hasil Analisis
Tran_BeratLahirBayi Pearson Correlation 1 .383*
Sig. (2-tailed) .041
N 29 29
L.WJ Pearson Correlation .383* 1
Sig. (2-tailed) .041
N 29 29
Descriptives
Statistic Std. Error
Interval Kehamilan Mean 26.08 6.488
95% Confidence Interval for
Mean
Std. Deviation 32.441
Minimum 0
Maximum 108
Range 108
Interquartile Range 39
Skewness 1.226 .464
Kurtosis .487 .902
Tinggi Badan Mean 158.28 1.011
95% Confidence Interval for
Mean
Lower Bound 156.19
Upper Bound 160.37
5% Trimmed Mean 158.23
Median 158.00
Variance 25.543
Std. Deviation 5.054
Minimum 148
Maximum 170
Interquartile Range 7
Skewness .320 .464
Kurtosis .224 .902
Hb Mean 11.144 .1664
95% Confidence Interval for
Mean
Lower Bound 10.800
Upper Bound 11.488
5% Trimmed Mean 11.111
Median 11.100
Variance .693
Std. Deviation .8322
Minimum 9.8
Maximum 13.1
Range 3.3
Interquartile Range 1.0
Skewness .683 .464
Kurtosis .436 .902
Descriptives
Statistic Std. Error
Usia Ibu Mean 29.00 .814
95% Confidence Interval for
Std. Deviation 4.383
Minimum 23
Maximum 38
Range 15
Interquartile Range 7
Skewness .366 .434
Kurtosis -.846 .845
Jenis Kelamin Bayi
Frequency Percent Valid Percent
Cumulative
Frequency Percent Valid Percent
Pendidikan
Frequency Percent Valid Percent
Cumulative
Percent
Valid Perguruan Tinggi 20 69.0 69.0 69.0
SD/sederajat 1 3.4 3.4 72.4
SMA/sederajat 7 24.1 24.1 96.6
SMP/sederajat 1 3.4 3.4 100.0
Total 29 100.0 100.0
Paritas
Frequency Percent Valid Percent
Cumulative
Percent
Valid 0 13 44.8 44.8 44.8
1 11 37.9 37.9 82.8
2 5 17.2 17.2 100.0
Lampiran 8. Dokumentasi
Lampiran 9. Biodata mahasiswa
Identitas
Nama : Alem Pramudita Wibowo
NIM : 22010113120094
TTL : Wonosobo, 28 Agustus 1995 Jenis Kelamin : Laki-laki
Alamat : Tawangsari Indah K-12 Wonosobo, Jawa Tengah Nomor HP : 082313131919
e-mail : [email protected]
Riwayat Pendidikan Formal
1. Staff Informasi dan Komunikasi HIMA KU Undip 2014/2015 2. Staff Membership and Development AMSA Undip 2014/2015
3. Kepala Bidang Informasi dan Komunikasi HIMA KU Undip 2015/2016 4. Executive Board of Asian Medical Students’ Exchange Program AMSA
Undip 2015/2016
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