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Lampiran 4. Prosedur pembuatan preparat

Judul : Korelasi Luas Area Wharton’s jelly dengan Luaran Berat Lahir Bayi pada Kehamilan Cukup Bulan

Tempat : Laboratorium Sentral RSUP Dr. Kariadi 1. Potong tali pusat sepanjang 2 cm dari ujung akhir

pemotongan, lalu masukkan kedalam container yang telah berisi Neutral Buffer Formalin dengan perbandingan 1:10. Beri Identitas dan bawa ke laboratorium Patologi Anatomi

2. Jaringan mentah dipotong menjadi 3 bagian (pilih bentuk potongan yang baik) lalu masukkan kedalam kaset. Lalu, masukkan kaset kedalam Neutral Buffer Formalin.

3. Pemasakan jaringan selama 17,5 jam :

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4. Jaringan yang telah melalui proses pemasakan kemudian dibuat menjadi blok jaringan menggunakan alat histocenter. Kemudian blok parafin didinginkan kedalam freezer.

5. Setelah didinginkan, blok jaringan kemudian dpotong menggunakan mikrotom. Hasil pemotongan dimasukkan kedalam air hangat lalu ditempelkan pada kaca objek. Beri identitas

6. Preparat kemudian dipanaskan dalam oven 70% selama 10 menit untuk melelehkan sisa parafin. Kemudian dinginkan dan tata di rak.

7. Proses pewarnaan dengan alat Veristein dengan total waktu 2,5 jam :

- Xylol 1, 2, dan 3 masing-masing 10 menit - Etanol selama 3 menit

- Alkohol 96% selama 3 menit - Alkohol 80% selama 3 menit - Alkohol 70% selama 3 menit - Alkohol 50% selama 3 menit - Bilas dengan air mengalir - Hematoksilin selama 15 menit - HCl selama 1 menit

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- Alkohol 50% selama 3 menit - Alkohol 70% selama 3 menit - Eosin selama 3 menit

- Alkohol 70% selama 3 menit - Alkohol 96% selama 3 menit - Etanol selama 3 menit

- Xylol 1, 2, dan 3 masing-masing selama 10 menit

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Lampiran 5. Lembar pengumpulan data

3. Pendidikan terakhir : a. Tidak sekolah d. SMA/sederajat b. SD/sederajat e. Perguruan Tinggi c. SMP/sederajat

REKAM MEDIS

1. Interval kehamilan : …… bulan 2. Usia Kehamilan : …… minggu

9. Riwayat gangguan fungsi jantung selama kehamilan?

a. Ya b. Tidak

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C. DATA BAYI

Jenis kelamin : Berat lahir :

D. HASIL PEMERIKSAAN HISTOFOTOMETRI

L.TP L.A1 L.A2 L.AT L.V L.PD L.WJ

mm2

Keterangan

L.TP : Luas area tali pusat

L.A1 : Luas area arteri umbilikal 1 L.A2 : Luas area arteri umbilikal 2

L.AT : Luas arteri umbilikal total (L.A1 + L.A2) L.V : Luas area vena umbilikal

L. PD : Luas area pembuluh darah (L.AT + L.V) L.WJ : Luas area Wharton’s jelly (L.TP – L.PD)

Semarang, 2016

Yang mengisi,

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Lampiran 6. Hasil Pemeriksaan

No Usia

Ibu Paritas Pendidikan

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Lampiran 6. Hasil Pemeriksaan (lanjutan)

No Usia

Ibu Paritas Pendidikan

Interval Kehamilan

Usia Kehamilan

Tinggi

Badan Hb

Jenis Kelamin Bayi

Berat

Lahir Bayi L.TP L.A1 L.A2 L.AT L.V L.PD L.WJ

28 31 1 Perguruan Tinggi 24 39 155 Laki-laki 3500 63.064 2.709 2.255 4.964 5.520 10.484 52.580

29 34 1 Perguruan Tinggi 24 38 158 10.4 Laki-laki 3900 49.217 1.438 2.497 3.935 3.120 7.055 42.162

30 36 2 SMA/sederajat 84 38 160 10.1 Laki-laki 3400 84.253 2.673 1.914 4.587 6.086 10.673 73.580

31 38 2 Perguruan Tinggi 108 39 160 12.2 Laki-laki 2900 59.083 3.954 3.058 7.012 5.165 12.177 46.906

34 32 2 SMA/sederajat 48 39 156 9.9 Laki-laki 2900 68.245 1.677 1.879 3.556 2.022 5.578 62.667

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Lampiran 7. Hasil Analisis

Tran_BeratLahirBayi Pearson Correlation 1 .383*

Sig. (2-tailed) .041

N 29 29

L.WJ Pearson Correlation .383* 1

Sig. (2-tailed) .041

N 29 29

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Descriptives

Statistic Std. Error

Interval Kehamilan Mean 26.08 6.488

95% Confidence Interval for

Mean

Std. Deviation 32.441

Minimum 0

Maximum 108

Range 108

Interquartile Range 39

Skewness 1.226 .464

Kurtosis .487 .902

Tinggi Badan Mean 158.28 1.011

95% Confidence Interval for

Mean

Lower Bound 156.19

Upper Bound 160.37

5% Trimmed Mean 158.23

Median 158.00

Variance 25.543

Std. Deviation 5.054

Minimum 148

Maximum 170

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Interquartile Range 7

Skewness .320 .464

Kurtosis .224 .902

Hb Mean 11.144 .1664

95% Confidence Interval for

Mean

Lower Bound 10.800

Upper Bound 11.488

5% Trimmed Mean 11.111

Median 11.100

Variance .693

Std. Deviation .8322

Minimum 9.8

Maximum 13.1

Range 3.3

Interquartile Range 1.0

Skewness .683 .464

Kurtosis .436 .902

Descriptives

Statistic Std. Error

Usia Ibu Mean 29.00 .814

95% Confidence Interval for

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Std. Deviation 4.383

Minimum 23

Maximum 38

Range 15

Interquartile Range 7

Skewness .366 .434

Kurtosis -.846 .845

Jenis Kelamin Bayi

Frequency Percent Valid Percent

Cumulative

Frequency Percent Valid Percent

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Pendidikan

Frequency Percent Valid Percent

Cumulative

Percent

Valid Perguruan Tinggi 20 69.0 69.0 69.0

SD/sederajat 1 3.4 3.4 72.4

SMA/sederajat 7 24.1 24.1 96.6

SMP/sederajat 1 3.4 3.4 100.0

Total 29 100.0 100.0

Paritas

Frequency Percent Valid Percent

Cumulative

Percent

Valid 0 13 44.8 44.8 44.8

1 11 37.9 37.9 82.8

2 5 17.2 17.2 100.0

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Lampiran 8. Dokumentasi

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Lampiran 9. Biodata mahasiswa

Identitas

Nama : Alem Pramudita Wibowo

NIM : 22010113120094

TTL : Wonosobo, 28 Agustus 1995 Jenis Kelamin : Laki-laki

Alamat : Tawangsari Indah K-12 Wonosobo, Jawa Tengah Nomor HP : 082313131919

e-mail : [email protected]

Riwayat Pendidikan Formal

1. Staff Informasi dan Komunikasi HIMA KU Undip 2014/2015 2. Staff Membership and Development AMSA Undip 2014/2015

3. Kepala Bidang Informasi dan Komunikasi HIMA KU Undip 2015/2016 4. Executive Board of Asian Medical Students’ Exchange Program AMSA

Undip 2015/2016

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