ABSTRACT
PEBRIAN INDRA RISKY DALIMUNTHE. Response Cylindrocladium sp. To the active substance Mancozeb Fungicide In Vitro. Guided by EDY BATARA MULYA SIREGAR and NELLY ANNA.
Cylindrocladium sp. is one of the pathogens trigger dangerous diseases that blight on eucalyptus plants in the pulp industry. Widely spread and prevention with the use of a contact fungicide mancozeb 80% WP.
Research on Response Cylindrocladium sp. To the active substanceMancozeb Fungicide In Vitro has been completed. The purpose of this study was to determine the response Cylindrocladium sp. on the provision of different concentrations and characterize the growth of colonies (shape, color, and texture). Research using completely randomized design (CRD) non factorial with basic media PDA. Concentrations used were 0 mg / ml (control) 0.4 mg / ml (M1), 0.8 mg / ml (M2), 1.2 mg / ml (M3), and 1.6 mg / ml (M4). Parameters measured were Cylindrocladium colony diameter sp., The percentage of colonies relative barriers Cylindrocladium sp., Spore density Cylindrocladium sp., Macroscopic and microscopic observation. Treatment significantly affect colony growth, relative barriers and spore density. Macroscopic characteristics include a white colony, the hyphae such as cotton, spread evenly and thicken in the middle. Microscopic characteristics which include changes in the structure of the hyphae, branching hyphae, density klamidiospora, Konia increasingly stunted and swelling
of such tumors in a set of hyphae.
Keywords: Response, In Vitro, Cylindrocladium sp., Fungicide Mancozeb 80% WP.
ABSTRAK
PEBRIAN INDRA RISKY DALIMUNTHE. Respon Cylindrocladium sp. Terhadap Fungisida Berbahan Aktif Mancozeb Secara In Vitro. Dibimbing oleh EDY BATARA MULYA SIREGAR dan NELLY ANNA
Cylindrocladium sp. adalah salah satu patogen pemicu munculnya penyakit berbahaya yaitu hawar daun pada tanaman ekaliptus pada industri pulp. Penyebarannya secara luas dan pencegahannya dengan penggunaan fungisida kontak mancozeb 80% WP.
Penelitian tentang Respon Cylindrocladium sp. Terhadap Fungisida Berbahan Aktif Mancozeb Secara In Vitro telah selesai dilakukan. Tujuan penelitian ini adalah menentukan respon Cylindrocladium sp. terhadap pemberian konsentrasi yang berbeda dan mengkarakterisasi pertumbuhan koloni (bentuk, warna, dan tekstur). Penelitian menggunakan Rancangan Acak Lengkap (RAL) non Faktorial dengan media dasar PDA. Konsentrasi yang digunakan adalah 0 mg/ml (kontrol) 0.4 mg/ml (M1), 0.8 mg/ml (M2), 1.2 mg/ml (M3), dan 1.6 mg/ml (M4). Parameter yang diamati adalah diameter koloni Cylindrocladium sp., Persentase hambatan relative koloni Cylindrocladium sp., kerapatan spora Cylindrocladium sp., pengamatan makroskopis dan mikroskopis. Perlakuan berpengaruh nyata terhadap pertumbuhan koloni, hambatan relatif dan kerapatan spora. Karakteristik makroskopis meliputi koloni berwarna putih, hifa seperti kapas, menyebar merata dan menebal di tengah. Karakteristik mikroskopis yang meliputi perubahan struktur pada hifa, percabangan hifa, kerapatan klamidiospora, konia yang semakin kerdil dan terjadi pembengkakan seperti tumor pada sekumpulan hifa.
Kata kunci: Respon, In Vitro, Cylindrocladium sp., Fungisida Mancozeb 80% WP.
