35 Induksi Kalus Embriogenik Pada Perbanyakan Leek (Reskhi Oktaviani, Endang Pudjihartati, Maria Marina Herawati)
1 Alumni Fakultas Pertanian & Bisnis Universitas Kristen Satya Wacana, E-mail: [email protected] 2 Dosen Fakultas Pertanian & Bisnis Universitas Kristen Satya Wacana, E-mail: [email protected] Jl. Diponegoro 52-60 Salatiga
3 Pengelola pembibitan Leek di Purwokerto
Reskhi Oktaviani1, Endang Pudjihartati2, Maria Marina Herawati2
Diterima 13 April 2012, disetujui 31 Juli 2012
INDUKSI KALUS EMBRIOGENIK PADA PERBANYAKAN LEEK (
Allium porrum
L.)
EMBRYOGENIC CALLUS INDUCTION ON THE MULTIPLICATION OF LEEK
(
Allium porrum
L.)
ABSTRACT
Multiplication of leek (Allium porrum L.) by tissue culture is an alternative to the provision of seedlings, especially if the varieties no tillering and not able to flowering during plant production in Indonesia. A study was conducted to determine the best various of explant and growth regulator composition to induce embryogenic callus. Primary somatic embryos obtained by planted explant leaf base and root tip on MS medium containing 2.4 D and BA. At a later stage, primary somatic embryos will form a secondary somatic embryo after subculture. 1 month old root tips are the best explants for induce embryogenic callus, while ZPT 1.0 mg / l 2.4 D + 1.0 mg / l BA was the most appropriate composition to induce embryogenic callus.
