Identitas Jurnal Ilmiah : a. Nama Jurnal : IOP Conference Series: Earth and

(1)

LEMBAR

HASIL PENILAIAN SEJAWAT SEBIDANG ATAU PEER REVIEW KARYA ILMIAH : JURNAL ILMIAH

Judul Jurnal Ilmiah (Artikel) : The Roles of Macrobenthic Molluscs as Bioindicator in Response to Environmental Disturbance : Cumulative k-dominance curves and bubble plots ordination approaches

Jumlah Penulis : Sapto P , Fuad M, Amalia A, Widowati, Suhartana/5 orang Status Pengusul : penulis utama/penulis anggota

Identitas Jurnal Ilmiah : a. Nama Jurnal : IOP Conference Series: Earth and

Environmental

b. Nomor ISSN : Online ISSN: 1755-1315;Print ISSN:

1755-1307

c. Volume, nomor, bulan tahun : Vol. 55, No. 012022, 2017, pp. 755-1315

d. Penerbit : IOP Publishing

e. DOI artikel (jika ada) : 10.1088/1755-1315/55/1/012022 f. Alamat web jurnal :

JURNAL : http://iopscience.iop.org/article/10.1088/1755-1315/55/1/012022 ARTIKEL : http://iopscience.iop.org/article/10.1088/1755-1315/55/1/012022/pdf

g. Terindeks di Scopus SJR (2017) 0.149;

https://www.scimagojr.com/journalsearch.php?q=19900195068&tip=sid&clean=0 Kategori Publikasi Prosiding : Prosiding Internasional Terindeks

(beri pada kategori yang tepat) Prosiding Internasional Prosiding Nasional Hasil Penilaian Peer Review :

Komponen Yang Dinilai

Nilai Reviewer

Nilai Rata-rata Reviewer I Reviewer II

a. Kelengkapan unsur isi jurnal (10%) 2,25 2,25 2,25

b. Ruang lingkup dan kedalaman pembahasan (30%) 7,50 7,50 7,50

c. Kecukupan dan kemutahiran data/informasi dan metodologi (30%)

7,50 6,75 7,12

d. Kelengkapan unsur dan kualitas terbitan/jurnal (30%) 5,25 6,75 6,00

Total = (100%) 22,50 23,25 22,87

Nilai Pengusul 2,25 2,32 2,28

Semarang, Agustus 2018

Reviewer 1 Reviewer 2

Prof . Dr. Ir Ambariyanto, M.Sc. Prof. Drs. Ocky Karna Radjasa, M.Sc., Ph.D.

NIP. 196104131988031002 NIP. 196510291990031001

Unit kerja : FPIK Undip Unit kerja : FPIK Undip

(2)

LEMBAR

HASIL PENILAIAN SEJAWAT SEBIDANG ATAU PEER REVIEW KARYA ILMIAH : JURNAL ILMIAH

Judul Jurnal Ilmiah (Artikel) : The Roles of Macrobenthic Molluscs as Bioindicator in Response to Environmental Disturbance : Cumulative k-dominance curves and bubble plots ordination approaches

Jumlah Penulis : Sapto P , Fuad M, Amalia A, Widowati, Suhartana/5 orang Status Pengusul : penulis utama/penulis anggota

Identitas Jurnal Ilmiah : a. Nama Jurnal : IOP Conference Series: Earth and

Environmental

b. Nomor ISSN : Online ISSN: 1755-1315;Print ISSN:

1755-1307

c. Volume, nomor, bulan tahun : Vol. 55, No. 012022, 2017, pp. 755-1315

d. Penerbit : IOP Publishing

e. DOI artikel (jika ada) : 10.1088/1755-1315/55/1/012022 f. Alamat web jurnal :

JURNAL : http://iopscience.iop.org/article/10.1088/1755-1315/55/1/012022 ARTIKEL : http://iopscience.iop.org/article/10.1088/1755-1315/55/1/012022/pdf

g. Terindeks di Scopus SJR (2017) 0.149;

https://www.scimagojr.com/journalsearch.php?q=19900195068&tip=sid&clean=0

Kategori Publikasi Prosiding : Prosiding Internasional Terindeks (beri pada kategori yang tepat) Prosiding Internasional

Prosiding Nasional Hasil Penilaian Peer Review :

Komponen Yang Dinilai

Nilai Maksimal Prosiding

Nilai Akhir Yang Diperoleh Prosiding

Internasional Terindeks

Prosiding Internasional

Prosiding Nasional

a. Kelengkapan unsur isi jurnal (10%) 3,00 2,25

b. Ruang lingkup dan kedalaman pembahasan (30%)

9,00 7,50

c. Kecukupan dan kemutahiran

data/informasi dan metodologi (30%)

9,00 7,50

d. Kelengkapan unsur dan kualitas terbitan/jurnal (30%)

9,00 5,25

Total = (100%) 30,00 22,50

Nilai Pengusul = 40% x 22,50 x1/4= 2,25 Catatan Penilaian artikel oleh Reviewer :

1. Kesesuaian dan kelengkapan unsur isi paper:

Penulisan sudah sesuai dengan ‘ Guide for Author’’ ( Title, Introduction, Materials and method, Results and Discussion, Conclusion, Acknowledgement, Reference) dengan system Author Unsur dalam artikel ditulis lengkap. Latar belakang riset komprehensif dan terstruktur dengan baik. Gambar/Grafik khususnya gambar 1,2 dan 4 nampak kurang besar

2. Ruang lingkup dan kedalaman pembahasan:

Ruang lingkup pembahasan baik, materi artikel berhubungan dengan aplikasi biomonitoring. Paragraf kesimpulan mengulang bagian hasil 3. Kecukupan dan kemutakhiran data/informasi dan metodologi:

Data diambil dengan metodologi yang memadai. Metode grafik dan ordinasi relevan untuk penentuan tingkat gangguan lingkungan 4. Kelengkapan unsur dan kualitas terbitan: .

Artikel dipublikasikan pada jurnal yang terindek scopus .

Semarang, Agustus 2018 Reviewer 1

Dr. Ir. Ambariyanto, M.Sc.

NIP. 196104131988031002

Unit kerja : FPIK Undip

(3)

LEMBAR

HASIL PENILAIAN SEJAWAT SEBIDANG ATAU PEER REVIEW KARYA ILMIAH : JURNAL ILMIAH

Judul Jurnal Ilmiah (Artikel) : The Roles of Macrobenthic Molluscs as Bioindicator in Response to Environmental Disturbance : Cumulative k-dominance curves and bubble plots ordination approaches

Jumlah Penulis : Sapto P , Fuad M, Amalia A, Widowati, Suhartana/5 orang Status Pengusul : penulis utama/penulis anggota

Identitas Jurnal Ilmiah : a. Nama Jurnal : IOP Conference Series: Earth and

Environmental

b. Nomor ISSN : Online ISSN: 1755-1315;Print ISSN:

1755-1307

c. Volume, nomor, bulan tahun : Vol. 55, No. 012022, 2017, pp. 755-1315

d. Penerbit : IOP Publishing

e. DOI artikel (jika ada) : 10.1088/1755-1315/55/1/012022 f. Alamat web jurnal :

JURNAL : http://iopscience.iop.org/article/10.1088/1755-1315/55/1/012022 ARTIKEL : http://iopscience.iop.org/article/10.1088/1755-1315/55/1/012022/pdf

g. Terindeks di Scopus SJR (2017) 0.149;

https://www.scimagojr.com/journalsearch.php?q=19900195068&tip=sid&clean=0

Kategori Publikasi Prosiding : Prosiding Internasional Terindeks (beri pada kategori yang tepat) Prosiding Internasional

Prosiding Nasional Hasil Penilaian Peer Review :

Komponen Yang Dinilai

Nilai Maksimal Prosiding

Nilai Akhir Yang Diperoleh Prosiding

Internasional Terindeks

Prosiding Internasional

Prosiding Nasional

a. Kelengkapan unsur isi jurnal (10%) 3,00 2,25

b. Ruang lingkup dan kedalaman pembahasan (30%)

9,00 7,50

c. Kecukupan dan kemutahiran

data/informasi dan metodologi (30%)

9,00 6,75

d. Kelengkapan unsur dan kualitas terbitan/jurnal (30%)

9,00 6,75

Total = (100%) 30,00 23,25

Nilai Pengusul = 40% x 23,25x1/4 =2,32 Catatan Penilaian artikel oleh Reviewer :

1. Kesesuaian dan kelengkapan unsur isi paper:

Sistematika artikel sesuai dengan instruction for Author ( Title, Introduction, Materials and method, Results and Discussion, Conclusion, Acknowledgement, Reference). Referensi perlu ditambahkan untuk menguatkan hasil dan pembahasan

2. Ruang lingkup dan kedalaman pembahasan:

Ruang lingkup pembahasan baik, dengan materi artikel berhubungan dengan komputer.. Kedalaman pembahasan secara scientific masih kurang, dan materi hasil lebih fokus pembahasan pada aspek aplikasi

3. Kecukupan dan kemutakhiran data/informasi dan metodologi:

Metodologi relevan dengan bidang ilmu ekologi perairan. Tidak ada rekomendasi utnuk bidang yang diberikan 4. Kelengkapan unsur dan kualitas terbitan: .

Artikel dipublikasikan pada jurnal terindek scopus dengan SJR (2017) 0.149

Semarang, Agustus 2018 Reviewer 2

Prof. Drs. Ocky Karna Radjasa, M.Sc., Ph.D.

NIP. 196510291990031001

Unit kerja : FPIK Undip

(4)

Sapto Purnomo Putro

(5)

Document details

30 of 39

The Roles of Macrobenthic Mollusks as Bioindicator in Response to

Environmental Disturbance: Cumulative k-dominance curves and bubble plots ordination approaches

(Conference Paper) (Open Access)

, , , ,

Center of Marine Ecology and Biomonitoring for Sustainable Aquaculture (Ce-MEBSA), Integrated Laboratory, Diponegoro University, Jl. Prof. H. Soedarto, SH, Semarang, 50275, Indonesia

Biology Department, Science and Mathematics Faculty, Diponegoro University, Prof. H. Soedarto, SH street, Semarang, 50275, Indonesia

Mathematics Department, Science and Mathematics Faculty, Diponegoro University, Prof. H. Soedarto, SH street, Semarang, 50275, Indonesia

Abstract

Floating net cage is one of the aquaculture practice operated in Indonesian coastal areas that has been growing rapidly over the last two decades. This study is aimed to assess the roles of macrobenthic mollusks as bioindicator in response to environmental disturbance caused by fish farming activities, and compare the samples within the locations using graphical methods. The research was done at the floating net cage fish farming area in the Awerange Gulf, South Sulawesi, Indonesia at the coordinates between ° ′- ° ′ LS and ° ′- ° ′ BT, at the polyculture and reference areas, which was located km away from farming area. Sampling period was conducted between October to June . The sediment samples were taken from the two locations with two sampling time and three replicates using Van Veen Grab for biotic and abiotic assessment. Mollusks as biotic parameter were fixed using

% formalin solution and were preserved using % ethanol solution after mm mesh size. The macrobenthic mollusks were found as many as species consisting of families and classes (gastropods and bivalves). Based on cumulative k-dominance analysis projected on each station, the line of station K T (reference area; ﬁrst sampling time) and KJAB P T (polyculture area; second sampling time) are located below others curves, indicating the highest evenness and diversity compared to the other stations, whereas station K T (reference area; ﬁrst sampling time) and K T (polyculture area, second sampling time) are located on the top, indicate the lowest value of evenness and diversity. Based on the bubble plots NMDS ordination, the four dominant taxa/species did not clearly show

involvement in driving/shifting the ordinate position of station on the graph, except T. agilis. However, the two species showed involvement in driving/shifting the ordinate position of two stations of the reference areas from the first sampling time by Rynoclavis sordidula, and three stations of the polyculture areas from the first sampling time by Gemulla sp. away from other stations, thus are considered as bioindicators of environmental disturbance. These results imply that cumulative k-dominance curves and bubble plots ordination may be effective method to assess the indication of disturbance of the area. © Published under licence by IOP Publishing Ltd.

SciVal Topic Prominence

Topic:

Prominence percentile: 93.494

Author keywords

bubble plots cumulative k-dominance Macrobenthic mollusk polyculture

◅ Back to results ◅ Previous Next ▻



^Export



^Download



^Print



^E-mail



Save to PDF

⋆

Add to List ^More...

▻

View at Publisher

IOP Conference Series: Earth and Environmental Science Volume 55, Issue 1, 1 March 2017, Article number 012022

2nd International Conference on Tropical and Coastal Region Eco Development 2016, ICTCRED 2016; Bali; Indonesia; 25 October 2016 through 27 October 2016; Code 126824

Putro, S.P.^a  Muhammad, F.^a Aininnur, A.^b Widowati^c Suhartana^d

a

b

c

 View additional aﬃliations



View references (18)



Benthos | Estuary | Biotic indices



PlumX Metrics

Usage, Captures, Mentions, Social Media and Citations beyond Scopus.

Metrics

3

Citations in Scopus

83rd percentile

1.65

Field-Weighted Citation Impact

Cited by 3 documents

, ,

(2020) International Journal of Innovation, Creativity and Change

, ,

(2018) Journal of Physics:

Conference Series

, ,

(2018) Journal of Physics:

Conference Series

Inform me when this document is cited in Scopus:

Brought to you by Universitas Diponegoro

Search Sources Lists SciVal ↗ Create account Sign in

(6)

Source details

IOP Conference Series: Earth and Environmental Science

Scopus coverage years: from 2010 to Present ISSN: 1755-1307 E-ISSN: 1755-1315

Subject area:

Earth and Planetary Sciences: General Earth and Planetary Sciences Environmental Science: General Environmental Science

View all documents

▻

Set document alert



Save to source list Journal Homepage

CiteScore 2018

0.44 ^

SJR 2018

0.170 ^

SNIP 2018

0.536 ^

CiteScore CiteScore rank & trend CiteScore presets Scopus content coverage

Calculated using data from 30 April, 2019

CiteScore

*CiteScore includes all available document types

0.44

⁼

Citation Count 2018 Documents 2015 -

2017*

=

Metrics displaying this icon are compiled according to , a collaboration between industry and academia.

2018

^

▻ 2,434 Citations

▻ 5,583 Documents

▻

View CiteScore methodology CiteScore FAQ

▻

Last updated on 09 April, 2020

CiteScoreTracker 2019

0.45

⁼

Citation Count 2019 Documents 2016 - 2018

=



Updated monthly

▻ 6,772 Citations to date

▻ 15,183 Documents to date

↗

Snowball Metrics

CiteScore rank

Category Rank Percentile

Earth and Planetary Sciences

#125/181 30th

Environmental Science

#143/192 25th



General Earth and Planetary Sciences

General Environmental

▻

View CiteScore trends

🔗

Add CiteScore to your site

About Scopus

What is Scopus Content coverage Scopus blog Scopus API Privacy matters

Language

⽇本語に切り替える切换到简体中文切換到繁體中文 Русский язык

Customer Service

Help Contact us

We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the .

↗

Terms and conditions Privacy policy ↗ Elsevier B.V ↗

use of cookies

Brought to you by Universitas Diponegoro

Search Sources Lists SciVal ↗ Create account Sign in

(7)

5/18/2020 Conference Policies

econference.undip.ac.id/index.php/ictcred/2016/about/editorialPolicies#custom-3 3/4

Important Dates

Abstract submission deadline : May 31^th, 2016 (extended to June 20, 2016)

Acceptance notification : August 1^st, 2016 Early bird registration : Before August 21^th, 2016 Full Text Submission : August 31^st, 2016

ICTCRED 2016 Conference : October 25^th- 27^th 2016

Keynote Speakers

Prof. Ocky Karna Rajasa

Director of Research and Public Services at Ministry of Research, Technology and Higher Education - Indonesia

"Policy on Coastal Research in Indonesia"

Prof. Ambariyanto

Diponegoro University - Indonesia

"Update in Coastal Development"

Prof. Makoto Tsuchiya University of the Ryukyus - Japan

"Coral Reef Ecosystem"

Prof. Chin Kun Wan

Chun Shan Medical University - Taiwan / President on International Society for Nutraceutical and Functional Foods

"Current Progress in Nutraceutical for Tropical Disease"

Prof. Peter Gell

Federation University of Australia - Australia

"Pollution and Coastal Environmental Changes in Tropical Region"

Prof. Johan C. Winterwerp TU Delft - Netherland

"Restoring eroding mangrove-mud coasts through Building with Nature"

Registration Fees

(8)

Scientific Committee/Editor

Hadiyanto (Diponegoro University, Indonesia) Makoto Tsuchiya (University of the Ryukyus, Japan) Heru Susanto (Diponegoro University, Indonesia)

Muhammad Mukhlisin (Semarang State Polytechnic, Indonesia) Jamari (Diponegoro University, Indonesia)

Hussein Gasem (Diponegoro University, Indonesia) Ambariyanto (Diponegoro University, Indonesia) Muhammad Zainuri (Diponegoro University, Indonesia) Muhammad Ali (Mataram University)

Sultana MH Faraz (Diponegoro University, Indonesia) Gerard Pals (Vrije Universiteit Amsterdam, Netherland)

Randi Hagerman (University of California Davis, United States) Flora Tassone (University of California Davis, United States) Herawati Sudoyo (Eijkman Institute, Indonesia) Ocky Karna Rajasa (MRTHE, Indonesia)

Craig Starger (Colorado State University, United States)

CN Ravishankar (Central Institute of Fisheries Technology, India) Agus Sabdono (Diponegoro University, Indonesia)

Maria Barbosa (Wageningen University, Netherland) Yasuhiro Igarashi (Japan)

Irwandi Jaswir (IIUM, Malaysia)

Michio Hidaka (University of the Ryukyus, Japan)

Organizing Committee

Munawar A Riyadi Chair

Tri Indah Winarni Co-Chair of TLS Diah Permata Wijayanti Co-Chair of CRED

Eko Susanto Secretary

Desrina Proceeding

Muh. Arfan Publication

This conference has been conducted by Institute of Research and Community Services, Diponegoro University, Indonesia 25-27 October 2016

2

2nd International Conference on Tropical and Coastal Region Eco Development 2016 IOP Publishing

IOP Conf. Series: Earth and Environmental Science 55

(2017) 011001 doi:10.1088/1755-1315/55/1/011001

(9)

5/15/2020 IOP Conference Series: Earth and Environmental Science, Volume 55, 2017 - IOPscience

https://iopscience.iop.org/issue/1755-1315/55/1 1/12

NOTICE: Ensuring subscriber access to content on IOPscience throughout the coronavirus outbreak - see our remote access guidelines.

investigated to assist with coastal management. Coastal erosion, which threatens public and private infrastructure, is one of the major problems along the harbour beaches. A study of sediment transport is essential to identify the challenges encountered by the stakeholders in coastal management. Darwin Harbour, located in the tropical, cyclone prone area of Australia, was, until recently, considered a near pristine estuary. A semi-diurnal macro-tidal embayment, the tidal variation in the harbour reaches up to 8 m with a mean tidal range of 3.7 m. The beach morphology consists of sandy pocket beaches between coastal cliffs, sandbars, rocky shore platforms, tidal flats and mangrove fringes. A two-dimensional depth averaged finite-element hydrodynamic model (RMA-2), coupled with a sediment transport model (RMA-11) from Resource Modelling Associates, has been used to infer the sources and the depositional areas of sand in the harbour. Grain size distributions and geochemical analysis are also used to characterize the sand and its source(s). Initial results show that the beach sand is mostly of offshore origin with small sand input from the rivers. Potential supplementary sand sources are the eroded materials from the shore platforms and the rocky cliffs. Due to the rapid development in Darwin Harbour, this study is fundamental in understanding coastal processes to support decision making in coastal management, particularly in a macro-tidal, tropical estuary.

Keywords: Darwin Harbour, macro-tidal, sand transport, coastal erosion, RMA

1. Introduction

Coastal erosion is a natural phenomenon. In fact, coast lines change continually, controlled by the interaction of the local hydrodynamics and their morphology. Coastal change is a longstanding problem that mankind has had to deal with to provide safety from flooding and to protect transportation infrastructure. Conventionally, coastal erosion is managed locally using hard engineering approaches, such as sea walls or breakwaters, which do not guarantee good outcomes and often create erosion in other areas [1, 2]. These consequences often stem from engineering decisions that only consider the immediately affected area, underestimating the processes that are occurring in the wider coastal zone.

1

2nd International Conference on Tropical and Coastal Region Eco Development 2016 IOP Publishing IOP Conf. Series: Earth and Environmental Science 55

(2017) 012012 doi:10.1088/1755-1315/55/1/012012

International Conference on Recent Trends in Physics 2016 (ICRTP2016) IOP Publishing Journal of Physics: Conference Series 755 (2016) 011001 doi:10.1088/1742-6596/755/1/011001

Content from this work may be used under the terms of theCreative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.

Published under licence by IOP Publishing Ltd

(13)

Past and Future Ecosystem Change in the Coastal Zone

P Gell¹

1

Water Research Network, Federation University Australia, Ballarat, Australia E-mail: p.gell@federation.edu.au

Abstract. The coastal zone is in a constant state of flux. Long term records of change attest to

high amplitude sea level changes. Relative stability though the Late Holocene has allowed for the evolution of barrier dune systems, estuaries and coastal lakes with associated plant and faunal associations. This evolution has been interspersed with changes in the balance between climate driven changes in outflow from catchments. These interactions have been considerably disturbed through the impacts of industrialised people who have diverted and consumed water and invested in infrastructure that has impacted on river flows and the tidal prism in estuaries. This has impacted their provisioning services to humans. It has also impacted their regulating services in that development along the coastline has impacted on the resilience of the littoral zone to absorb natural climate extremes. Looking from the past we can see the pathway to the future and more easily recognise the steps needed to avoid further coastal degradation. This will increasingly need to accommodate the impacts of future climate trends, increased climate extremes and rising seas. Coastal societies would do well to identify their long term pathway to adaptation to the challenges that lie ahead and plan to invest accordingly.

Keywords. estuaries, paleolimnology, climate change, hydroecology, sediments, nutrients,

salinization

1. Introduction

The coastal zone is in a constant state of change. This is most evident through the daily cycles of the tides that, particularly in the macrotidal zones of the world, inundate and then strand the marine littoral zones. This alone demands that the biological communities affected by the rise and fall of tides be adaptable to inundation and exposure, and in estuaries, exposed variously by marine waters and those flowing from the hinterland which are usually fresh to oligosaline. This balance varies seasonally with, in temperate zones, wet seasons coinciding with destructive waves regimes leading to the opening of estuary mouths, while the dry season and associated constructive wave regimes lead to mouth closure and the establishment of lagoonal conditions. The consequences are a winter of tidal and river flushing with oxygenated water and a summer with little flow, stratification, hypersalinity and de-oxygenation.

These seasonal patterns are taken to extreme under multi-year (e.g. El Nino Southern Oscillation) and even multi-decadal (Inter-decadal Pacific Oscillation) phases of wet and dry conditions exacerbating or subduing the contrasts between seasons. Further the frequency of these cycles extends out to multi-

1

2nd International Conference on Tropical and Coastal Region Eco Development 2016 IOP Publishing IOP Conf. Series: Earth and Environmental Science 55

(2017) 012001 doi:10.1088/1755-1315/55/1/012001

International Conference on Recent Trends in Physics 2016 (ICRTP2016) IOP Publishing Journal of Physics: Conference Series 755 (2016) 011001 doi:10.1088/1742-6596/755/1/011001

(14)

Antioxidant activity of three microalgae Dunaliella salina, Tetraselmis chuii and Isochrysis galbana clone Tahiti

Ita Widowati

¹

, Muhammad Zainuri

¹

, Hermien Pancasakti Kusumaningrum

²

, Ragil Susilowati

³

, Yann Hardivillier

⁴

, Vincent Leignel

⁴

, Nathalie Bourgougnon

⁵

, Jean-Luc Mouget

⁴

1

Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Jl. Prof. Soedarto SH, Tembalang, Semarang -50275 , Indonesia

2

Department of Biology, Faculty of Sciences and Mathematic, Diponegoro University, Jl. Prof Soedarto SH, Tembalang, Semarang -50275, Indonesia

3

Alumny Master of Coastal Ressource Management, Faculty of Fisheries and Marine Science, Diponegoro University, Jl. Prof.Soedarto SH Tembalang, Semarang-50275 , Indonesia

4

Laboratoire du Mer Molécule et Santé, Université du Maine, Le Mans, France

5

Laboratoire de Chimie Biologie Marine, Université de Bretagne Sud, Vannes, France

Email : ita_jusup@yahoo.co.id

Abstract. Natural alternatives antioxidant source has become a trending topic in the past decades to replace synthetic antioxidant. Microalgae have been mentioned to show interesting bioactive properties and one of them is its antioxidant activity. This study aims to evaluate the potential of three microalgae Dunaliella salina, Tetraselmis chuii and Isochrysis galbanaas new source of natural antioxidant. Proximate analysis and total phenolic content of D. salina, T. chuii and I.

galbanas were determined. Antioxidant activity of methanolic extracts of these three species prepared in different concentration (50, 100, 250, 500, and 1000 ppm) was performed through DPPH assay. I. galbana clone Tahiti demonstrated a highest antioxidant potential with 61.64 of inhibition at 50 ppm followed by D. salina with 58.45 % of inhibition and T. chuii with 52.58 % of inhibition. I. galbana clone Tahiti was the best antioxidant with total phenol content of 17.798 mg GAE g

^-1

extract at 50 ppm; followed by T. chuii 16.868 mg GAE g

^-1

extract and the lowest was D. salina with 4.672 mg GAE g

^-1

extract. Results suggest that these microalgae posses antioxidant potential which could be considered for future applications in medicine, dietary supplements, cosmetics or food industries.

Keywords: antioxidant activity, DPPH, microalgae, total phenol.

1. Introduction

Indonesia, as an archipelagic state surrounded by ocean, presents a potential as a microalgae source because of its huge natural biodiversity. Microalgae are distributed everywhere: in salt, brackish or freshwater, in tropical to cold regions and sometimes as symbionts with other organisms, and some species are used and cultivated by farmers.

1

2nd International Conference on Tropical and Coastal Region Eco Development 2016 IOP Publishing IOP Conf. Series: Earth and Environmental Science 55

(2017) 012067 doi:10.1088/1755-1315/55/1/012067

International Conference on Recent Trends in Physics 2016 (ICRTP2016) IOP Publishing Journal of Physics: Conference Series 755 (2016) 011001 doi:10.1088/1742-6596/755/1/011001

(15)

Study on Anticancer Activity of Extracts of Sponges Collected from Biak Water, Indonesia

A.Trianto

^1,2

, A. Ridhlo

²

, D.W. Triningsih

²

, and J. Tanaka

³

1 Natural Product Laboratory, Centre of Research and Services (Laboratorium Terpadu) Diponegoro University,

2 Department Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, Indonesia

3 Department Chemistry, Biology, and Marine Science, University of The Ryukyus, 1-Senbaru, Nishihara, Okinwa, Japan.

E-mail:

a g u s t r i a n t o . u n d i p @ g m a i l . c o m

Abstract. Indonesia is center of biodiversity where marine sponges are abundant. a source of bioactive compounds with various pharmaceutical properties such as anticancer, antifungal, antibacterial, antioxidants, anti-inflammatory, and anti-malarial. In a continuation of a search for biologically active molecules from marine organisms we investigated the potency of marine sponges as anticancer. A total of 106 sponge specimens were collected between 3-40 m depths by SCUBA diving in Biak waters during August 2005. The specimens were extracted with methanol to provided crude extracts. The methanolic extracts were tested against NBT-T2 cell line. The assay result showed that 8.5 %, 29.2 % and 46.2

% of the extract have activity against the cell line at 0.1, 1.0 and 10.0 g/mL. While, a 16.0

% of the extract did not showed activity against the cell line.

Keywords : sponge, anti-cancer, NBT-T2, screening, extract

1. Introduction

Sponges are the most primitive of multicellular invertebrate that distribute widespread in the marine ecosystem. Sponges are sessile animal with commonly have soft body that easy to be predated by various animal such as such as ﬁshes, turtles, sea urchin, nudibranches, and ﬂat worms [1][2].

Sponges also compete for space either with other sessile organisms or fouling organisms. For their survival sponges produce various classes of secondary metabolites such as alkaloid, steroid, glycosides, macrolides, and terpenoid [3].

Many researches showed that the secondary metabolites from sponges have pharmacological properties as anticancer, antibacterial, antifungal, antioxidant and other activities [4][5][6][7].

Cancer cells develop the resistance against the drug basically in two method i.e. by develop the membrane cell and development pumping system or known as mdr type 1 and mdr type 2. On the mdr type 1 the cell membrane will selectively pass the substrate that enter to cell. Once the cell recognize the anticancer drug the membrane will refuse it [8].

Marine organisms have proven to be an exceptionally valuable resource for bioactive natural products discovery efforts, particularly in the area of potential anticancer compounds. Metabolites isolated from marine samples often possess unique structural features and incorporate new or unusual assemblages of functional groups. They have demonstrated a broad range of potent biological

1

2nd International Conference on Tropical and Coastal Region Eco Development 2016 IOP Publishing IOP Conf. Series: Earth and Environmental Science 55

(2017) 012069 doi:10.1088/1755-1315/55/1/012069

International Conference on Recent Trends in Physics 2016 (ICRTP2016) IOP Publishing Journal of Physics: Conference Series 755 (2016) 011001 doi:10.1088/1742-6596/755/1/011001