MODULE 4: Microbiology (4.17 ECTS)
Degree Programme: Undergraduate Programme in Pharmaceutical Science & Technology Title of Module: Microbiology
Abbreviation: MB
Title of course: FA2114 Pharmaceutical Microbiology
Semester: 3
Responsible for module: Dr. Marlia Singgih Wibowo
Lecturer: Dr. Debbie S Retnoningrum
Dr. Marlia Singgih Wibowo
Dr. rer. nat. Catur Riani
Dr. Elin Julianti Language: Indonesian & English
Assignment to Programme: Undergraduate Programme in Pharmaceutical Science and Technology, Compulsory Class, Semester 3
Class Type/Hours: Lecture / 2h per week, practicum / 4 h per week weeks, tutorial / 1h per week
Workload: Study time in class : 32h
Laboratorium activity : 48h
Study time at home : 64h
Credits: 4.17 ECTS
Requirements: Registered to Undergraduate Programme in Pharmaceutical Science and Technology
Study Goals: Knowledge:
Describe and explain basic microbiology knowledge, methods and its application in pharmacy.
Skills:
Apply and demonstrate aseptic work, sterilization process, identification bacteria with conventional methods, calculate bacteria number in samples, determine and measure antibiotic potency, demonstrate PCR, electrophoresis of DNA & protein.
Competence:
Analyze and interpret data of basic microbiology methods.
Summarize scientific article content in written report and presentation.
Content: Structure, function and composition of prokaryotic cells (eubacteria &
archaebacteria) & virus; growth of microbe & virus; diversity of microbe &
virus; genetics of microbe; role of microbiology in Pharmacy; uniqueness of microbes (Presentation of written assignment); structure, function &
diversity of fungi and algae; structure, function & diversity of protozoa;
deterioration caused by microbe; microbial preservation & storage; standard quality of microbiology & basic of microbial control; microbiology assay for
pharmaceutical products; laboratory safety, audit & risk assessment;
secondary metabolite bioactive of marine microorganism for drug discovery.
Laboratory work modul: medium preparation and sterilization of instrument;
aseptic technique and inoculation methods, preparing subculture on solid and liquid medium; isolation,identification and confirmation microbes (Gram Staining); microbial contamination/ limit test and sterility test; determination of potency of antimicrobial agent; polymerase chain reaction (PCR); DNA and protein electrophoresis.
Assessment: Mid semester exam, final exam, posttest, presentation, written assignment, class participation, lab report, lab exam
Media: Slides, Guest lecture from vaccine industry, Laboratory practice Literature: Madigan, M.T., et al., Brock Biology of Microorganisms, 8th ed.,
Prentice Hall Int.Inc., 2006 , and new edition
Denyer SP, Baird RM, Guide to Microbiological Control in
Pharmaceuticals and Medical Devices , CRC Press, New York, 2007
Denyer S.P., Hodges N.A., Gorman S.P., Hugo and Russell’s Pharmaceutical Microbiology, 7th ed., 2004, Blackwell Science, Massachusetts.
Deacon J.W, Modern Mycology, 3rd ed., Blackwell Science, Edinburgh, 1997
Prave, P. et al.(ed), Basic Biotechnology : a Student’s guide, VCH, New York, 1987
Neidleman S.I, and Laskin A.I., Advances in Applied Microbiology, Academic Press, New York, 1997.
Nikapitiya, C., Bioactive Secondary Metabolites from Marine Microbes for Drug Discovery. In Advances in Food and Nutrition Research; Kim, S.-K., Ed; Academic Press: San Diego, 2012; Vol. 65, Chapter 24, pp 363-387.
Farmakope Indonesia ed.4, DepKes RI, Jakarta, 1995.
