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مقایسه خصوصیات بیولوژیکی و مولکولی جدایه ویروس رگه‌ای توتون (Tobacco streak virus) جدا شده از مزارع آفتابگردان ایران با جدایه‌های هندی و سودانی

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E-mail: samin_hf@yahoo.com

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ﺑ تﺎ ﻴ ژﻮﻟﻮ ﻳ ﻜ ﻲ ﻟﻮﻜﻟﻮﻣ و ﻲ

اﺪﺟ ﻳ ﻪ

و ﻳ ﻪﮔر سوﺮ ا

ي نﻮﺗﻮﺗ

(Tobacco streak virus)

زا هﺪﺷ اﺪﺟ

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43R (Local lesion/systemic ) Aizoaceae Tetragonia tetragonoidesL. CLL/M --/ -/-

Chenopodiaceae Chenopodium quinoa L. CLL/M CLL/M;W CLL/-

C. amaranticolor L. CLL/M -/- CLL/-

C. murale L. CLL/- --/ CLL/-

C. foliusum L. CLL/M CLL/M;W CLL/-

Copmositae Lactuca sativa L. -/- --/ -/-

Helianthus annus L. NLL/M -/M;W -/M

Cucurbitaceae Cucumis melo L. CLL/- --/ -/-

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Leguminosae Phaseolus vulgaris L. -/- --/ -/-

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Solanaceae Datura metel L. NLL/M; D --/ NLL/-

Datura stramonium L. NLL;CLL/M; DC --/ NLL/- Lycopersicon esculentum Mill. NLL/M;W --/ -/- Nicotiana benthamiana L. -/M; DC --/ -/L

Nicotiana clevelandii L. NLL/M --/ -/-

Nicotiana debneyi L. NLL/M --/ -/L

Nicotiana glutinosa L. NLL/M;R; DC --/ -/L

Nicotiana hesperis L. NLL/M;W --/ NLL/L

Nicotiana rustica L. NLL/M; DC;W --/ -/-

Nicotiana occidentalis L. NLL/M; DC;W --/ -/L Nicotiana tabacum cv. Samsun L. NLL/M;W --/ -/L Nicotiana tabacum cv. Xanthi L. NLL/M;R;W --/ -/L Nicotiana tabacum cv. White Burley L. NLL/M;W --/ -/-

Physalis floridana L. NLL/M --/ NLL/-

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REFERENCES

1. Abtahi, F. & Koohi Habibi, M. (2009). Host range and some characterization of TSV isolated from lettuce in Iran. African Journal of Biotechnology, 7 (23), 4260-4264.

2. Ali, M. A., Winter, S. & Dafalla, G. A. (2008). Tobacco streak virus infecting faba bean reported for the first time. The British Society for Plant Pathology, 144.

3. Almedia, A. M. R., Sakai, J., Hanada, K., Olivera, T. G., Belintani, P., Kitajima, E. W., Souto, E. R., Novaes, T. G. & Nora, P. S. (2005). Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil. Fitopatologia Brasileira, 30, 366-373.

4. Berguland, D. R. (2007). Sunflower production. Extension Publication. 120pp.

5. Clark, M. F. & Adams, S. A. N. (1977). Characteristics of microplates method of enzyme-linked- immunosorbent assay for detection of plant viruses. Journal of General Virology, 34, 475-483.

6. Dijkstra, J. (1983). Tobacco streak virus in sunflower (Helianthus annus). Netherland Journal of Plant Pathology, 89, 153-169.

7. Greber, R. S. (1971). Some characteristics of Tobacco streak virus isolates from Queesland. QueensI.

Journal of Agriculture and Animal Science, 28, 105-114.

8. Greber, R. S., Klose, M. J., Teakle, D. S. & Milne, J. R. (1991). High incidence of Tobacco streak virus in tobacco and its transmission by Microcephalothrips abdominalis and pollen from Ageratum houstonianum. Plant Disease, 75, 450-452.

9. Hosseini, S., Mosahebi, G. & Koohi Habibi, M. (2006). Distribution of sunflower viruses of Iran. In:

Proceedings of the 10th International Plant Virus Epidermiology Symposium, 15-19 Oct. Hyderabad, India, P. 2_98.

10. Jordan, R. L. (1990). Strategy and techniques for the production of monoclonal antibodies; monoclonal antibody applications for viruses. In serological methods for the detection and identification of viral and bacterial plant pathogens. A Laboratory Manual. APS press. 389pp.

11. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.

12. Motamedi, M. (2008). Characterization of TSV and TSWV isolated from sunflower. M. Sc. dissertation, University of Tehran, Iran (In Farsi).

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13. Milne, R. & Luisoni, E. (1977). Rapid immune electron microscopy of virus preparations. Methods in Virology. Vol. 6. Academic Press, New York. 265-281.

14. Rao, R. D. V. J. P, Reddy, A. S., Reddy Thirumala-Devi, S. V., Rao, S. C., Kumar, V. M., Subramaniam, K., Reddy, T. Y., Nigam, S. N. & Reddy, D. V. R. (2003). The host range of Tobacco streak virus in India and transmission by thrips. Annual Application Biology, 142, 365-368.

15. Ravi, K. S., Buttegereitt, A. S., Kitkaru, A. S., Deshmukh, S., Lesemann, D. E. & Winter, S. (2001).

Sunflower necrosis disease from India is caused by an ilarvirus related to Tobacco streak virus. Plant Pathalogy, 50, 800.

16. Reddy, A. S., Prasada Rao, R. D. V. J., Thirumala-Devi, K., Reddy, S. V., Mayo, M. A., Roberts, I., Satyanarayana, T., Subramaniam, K. & Reddy, D. V. R. (2002). Occurrence of Tobacco streak virus on peanut (Arachis hypogaea) in India. Plant Disease, 86, 173-178.

17. Sambrook, J., Fristch, E. F. & Maniatis, T. (1989). Molecular cloning a laboratory manual. (2nd ed.).

Cold Spring Harbor Laboratory. Cold Spring Harbor, New York.

18. Sambrook, J. & Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual. (3rd ed.). Cold Spring Harbor Laboratory Press, NY.

19. Sharman, M., Thomas, J. E. & Persley, D. M. (2008). First report of Tobacco streak virus in sunflower, cotton, chickpea and mung bean in Australia. Australas. Plant Disease, 3, 27-29.

20. Sharman, M., Persley, D. M. & Thomas, J. E. (2009). Distribution in Australia and seed transmission of Tobacco streak virus in Parthenium hysterophorus. Plant Disease, 93, 708-712.

21. Towbin, H., Staehelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some Applications Academic Science, 76, 4350-4354.

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