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( DOI: 10.22059/ijhs.2020.302255.1800
* Corresponding author E-mail:[email protected]
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Effect of Hana leaf extract and sodium nitroproside on some physiological characterestics and vase life of chrysanthemum cut flower
(Chrysanthemum morifolium)
Sadaf Noorani Niaki1, Raheleh Ebrahimi2*, Orang Khademi3 and Foad Fatehi4
1, 2. M. Sc. Graduate and Assistant Professor, Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran
3. Associate Professor, Faculty of Agriculture, Shahed University, Tehran, Iran 4. Associate Professor, Department of Agriculture, Payame Noor University, Tehran, Iran
(Received: May 05, 2020- Accepted: Oct. 04, 2020)
ABSTRACT
In order to investigate the effect of Hana extract and sodium nitroprusside on antioxidant properties and vase life of chrysanthemum, an experiment as factorial in a completely randomized design with three replications was conducted. The first factor was Hana leaf extract at three levels (0, 100 and 200 ppm), the second factor was sodium nitroprusside at three levels (0, 0.5 and 1 mM) and the third factor was sampling time at three levels (1, 6 and 12 day). The results showed that with increasing sampling time, flower diameter, chlorophyll content, relative water content and relative fresh weight decreased and the activity of antioxidant enzymes increased significantly. The extract of hana and sodium nitroprusside at high concentrations reduced the activity of antioxidant enzymes and improved chrysanthemum quality parameters, indicating their positive effects on the elimination of free radicals. The Hana extract treatment on sixth day of sampling time was the best treatment for improving the parameters of chrysanthemum flower. The Hana leaf extract and sodium nitroprusside treatments suppressed reduction of chlorophyll a, chlorophyll b and total chlorophyll leaf content. The results of this study showed that hana extract and sodium nitroprusside had positive effect on vase life of chrysanthemum cut flower.
Keywords: Antioxidant enzymes, chlorophyll content, relative water content.
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Table 1. Results of variance analysis of Hana leaf extract, sodium nitroproside and sampling time on chlorophile content, and antioxidante enzymes of chrysanthemum cut flower.
Mean of squares.
Polyphenol oxidase
Ascorbate peroxidase
Catalyse
Total chlorophile
Chlorophile b
Chlorophile a
d.f.
Source of variation
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0.09 1
Sampling time (S)
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0.00009ns 4
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0.0004ns 2
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0.00008ns 0.0003ns
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0.00066 0.00066
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