XUMUR npUpOOHblX coeouHeHuu, 2008, M!2 207
YAK 547.913
KOMTIOHEHTHbIH COCTAB II AHTHMHKPOJ>HAH AKTIIBHOCTb 3<f)IIPHOrO MACJIA ARTEMISIA KASAKORUM
E.M. CY/leiiMeHosa,T. Ozelt, RDemircf, B. Demirci6, K.H. C Baser"',CM. Aoe«eHos8
a) E6pmuucKuu HatfUOHaJlbHbluYHu6epcumem lW. JI.H. rYMUJle6a, 010008, AcmaHa, MYHaumnaco6a, 5, mell.: +7(7172) 353806, e-mail: [email protected]
6) C/JapMal1e6mU'leCKuupaKYJ/bmem YHU6epcumema AHaoo.IlY,26470 3cKuzuexup, TyptfUR 6) AD HIIlJ; «C/JumOXUMUJl», 100019,2. Kapa2aHoa, ra3aJ1Ue8a, 5,
paKc: 8(3212) 43 37 73, meJ/.: 8(3212) 43 31 27, e-mail: [email protected]
Artemisia kasakorum (Krasch.) Pavlov (nOJIblHb Ka3axcKaH) - KopHeBH~HbIH cepoBaTO-3eJIeHblH
MHOrOJIeTHHK BblCOTOH )].0 80 CM C HeMHOrOtIHCJIeHHblMH TOJICTOBaTbIMJiI KpenKJilMJiI reHepaTHBHbIMH n06eraMJiI.3H)].eMHtIHbIH TypaHcKHH BH)]., 3KOJIOrHtIeCKJiI npHypO'IeHHbIM K CJilJIbHO 3aCOJleHHblM Tpen'l'IHbIM rJIJilHaM, nYXJIbIM, MOKpbIM JiI JIyroBblM COJIOH'IaKaM. ,I:{aHHbIH BJiI)].
6JIJil30K K CJil6Ji1pCKOMY cTenHoMY BH)].Y - nOJIWHH
CCJIJilTpHHOM (A. nitrosa Web.) [I]. H. B. ilaBJIOB
paccMaTpHBaJI A. kasakorum B KatIeCTBe caMOCTOHTeJIbHOrO BJiI)].a. Ha6JIIO)].eHHH B npHpo)].e JiI C60PbI )].JIH rep6apJilll nOCJIe)].HJilX JIeT nOMOrJIH BbIlIBJilTb era 3KOJIOm1JecKYIO H reorpaqmtIecKYJO npHypOqeHHOCTb, qTO n03BOJIHJIO OTHeCTJiIera K na.ne03H)].eMI1'IHoMY BH)].y C ceBepo-rypaHCKJilM THnOM
apeana [2].
B03)].yIIIHo-cyxoe cbIpbe A. kasakorum C06Ji1panH B OKp.
noceJIKa ,I:{oranaH CeMHnanaTHHCKOHo6naCTH B HatIane aBryCTa 1994 r. H)].eHTHcpHKau.110HHbIH 06paJeu. (N!!1989.07.11.05.16)
XpaHHTCH B <poH)].e rep6apH1l AO I-II1I..J:«<!>JilTOXJilMJiIH».3<pJilpHOe MaCJIO nOJIyqanJil MeTO)].OM rH)].pO)].HCTJilJIJIHUHHB Te'IeHHe2
tIHa annapaTe KneBeH)])Kepa J13 Ha)].3eMHOH 'IaCTH A. kasakorum, H3MeJIb'IeHHOH)].0 'IaCTHU paJMepoM 3-5 MM. BbIXO)].3<pHPHOro MaCJIa 0.12% B nepeC1JeTe Ha B03)].yIIIHo-cyxoe Cblpbe.
XpOMaTO-Macc-cneKTpOMeTpH'IeCKHH aHaJIH3 3<pHpHoro Macna npOBO)].HJIH B YCJIOBI1HX, aHanOmtIHblX YCJIOBI1HM, OnJilCaHHbIM B [3].
TAEJU1l\A I. KOMnOHeHTHblil COCT80 3"'HpHoro MaCJla A. kasakorum
HHACKC
KOMnOHeHT HHAeKc
KOMnOHeHT
I
COAepJKa-
YAep)K. Y.l\CpJK. HHe, %
1032 a-nHHCH 0.1 1616 XOTpHeHOn 0.5
1035 a- TyileH 0.1 1625 4,4- AHMeTHJ16ymn-2-eHonHJI, 1.7
1076 KaM<!>eH 0.3 1638 lIuC-p-MeHTa-2-eH-1-o. 0.6
J 100 YH.l\eKaH 0.1 1642 Tyi1-3-eH-10-am, 0.7
11\8 -nHHeH 0.1 1648 MHpTeHaJlb 0.1
\132 Ca6HHeH 0.1 1658 Ca6HHHJlaQeTaT 12.9
1188 a-TcpnHHelJ 0.3 1670 2,6,10,14- TeTpaMeTHJlneHTaJ.\eKaH**
3.0
1\95
AerH.l\po-1,8-uHHeon0.2 1685 3mn6eH30aT 0.5
1213
1,8-IJ,HHeoJl*4.3 \700 fellT8.l\eKaH 1.1
1244
AMHJI<!>ypaH 0.3 1703 He H.l\eHT.1 2.41255 y- TepnHlJelJ 0.9 1717 He H.l\eHT. 2
2.4
1280 p-IJ,HMeH 3.4 1720
mpallc-CafiHHOJl4.6
1294
1,2,4- TpHMeTHn6eH30Jl1.1 1773 o-KMllHelJ 0.2
1300 TpH.l\eKaH 0.3 1778
He HJI,eHT. 32.2
1355 1,2,3-TpHMemJl6eIJ30Jl 0.7 1792
He H.l\eHT. 42.0
1400
TeTpMeKaH0.6 1802
KYMHIJOB£'Ii1anbJl,erH.l\2.4
1437 a-Tyi1olJ 2.7 1821
He H.l\eIJT. 5 2.41443 2,5- AHMeTHJlCmpeH 0.2 1838 (E)-[1-AaMaCKeIJOH OJ
1445 <l>H.H<!>OJlOH 0.1 1858 He H.l\eHT.6 2.5
1451 -Tyi10H 3.0 1900 HOlJaJleKalJ 1.1
1474 mpaHc-Ca6HHelJrHJl,paT 0.2 1943 He Hl1eHT. 7 \.4
1480 HeponoKcH.l\ 0.1 2000 3i1KOJaIJ 1.8
1500 neHTMeKaH 1.3 2037 He H.l\eIJT. 8
2.8
1506 AeKaHanb 0.1 2050
(E)-HepoJlH.l\on0.8
1532 KaM"'opa 3.7 2131
f eKCarHApo<!>apHe3Hnal.\eTOH Cn.\553 JIHlJaJlOOn 0.2 2144 CnaTyJleHOn 1.7
1573 mpaHc-p-MeHTa-2-eH-I-oJl 0.4 2753 AHTpauelJ 0.7
1582 IIUC-XpH3aHTeHHnaueTaT 0.9 2858 He H,lI,eIJT. 9 0.4
\586 nHHoKapBoH 0.3 2869 He HJl,elIT.10 0.8
1597 EopHHnaueTaT 0.1 2919 He HJI,eHT. I1 0.1
1600 feKCaJleKaH 0.5 2923 He HJI,eIJT. 12 0.4
1611 TepnHlJelJ-4-on 2.5 293\ feKC8.l\eKaIJOBaJlKHcnOTa 2.3
HTOro
86.1
* : OCHOBlJbleKOMnOHeHTblBbll1eJleHbIJKMpIJblMrnpH<!>TOM;
**: onpeJl,enelJO no HHAeKcy Y.l\epJKHOaIJHJIH no 6M6nHoTeKe Wiley
208 XWHUR npUpOOHblX coeouHeHuu, 2008, M2
l1,!1,eHTIUpHIJ.HpOBaJII1 52 KOMflOHeHTa, COCTaBHBllIHX 66.2%
OT Bcex o6HapY)I{eHHbIX KOMflOHeHTOB. OCHOBHbIMH KOMnOHeHTaMH3qJHpHOrO Macna A. kasakorum HBJUIIOTCH ca6HHHJlau.eTaT-12.9%, mpaHc-ca6HHon-4.6, 1,8-u.HHeoJl- 4.3, KaMcpopa- 3.7, n-u.HMeH- 3.4 H ~-TYHOH - 3.0%.
CO,!l,ep)((aHHe yrJleBO,!l,OpO,!l,OB H KHCJ10P°,!l,ocoJ],ep)((aIlU1X COe,!l,HHeHHH
-
COOTBeTCTBeHHO13.9
H47.1%.
OCHoBHaH (44.3%) IfaCTb 3cpHpHoro MaCJ1a - MOHoTepfleHOH,!I,bI H nHllIb 3.1 % COCTaBJ1HIOTcecKBHTepneHOH,!I,bI.KOMnoHeHTHbIH COCTaB 3cpHpHoro MaCJ1aA. kasakorum
flpe,!l,CTaBJleH B Ta6J1. I.
He HH,!I,. I: ElMS, 70 eV,m/z (%rel.int): 189(0.3),134(6), 119(9),108(4),92(49),91(100),71(11),59(9),43(30).
He HHJ],. 2: ElMS, 70 eV,mlz (% reI.int): 224(0.7),193(0.4), 134(5),119(8),108(10),92(48),91(100),57(23),41(5).
He HH,!I,. 3: ElMS, 70 eV,m/z (% reI. int): 253(0.6), 197(5), 183(5),169(2),155(3),141(4),127(12),113(12),99(12),85(42), 71(84),57(100),43(47).
He HH,!I,. 4: ElMS, 70 eV,mlz (% reI.int): 220(0.4), 177(0.3), 165(0.4),153(0.4),134(7),119(10),105(3),92(5), 92(5),91(100), 85(6),57(24),41(13).
He HH]],. 5:ElMS, 70 eV, mlz (% reI.int): 221(0.3),193(0.1), 165(0.4),152(0.4),134(8), 119(10),108(7),92(51),91(100),85(10), 57(16),43(12).
He HH,!I,. 6: ElMS, 70 eV,m/z (% reI. int.): 250( 1),211 (3), 183(7),153(9),141(10),113(12),99(17),85(41), 71(76),57(100), 43(70).
He HH,!I,. 7: ElMS, 70 eV, mlz (% reI. int): 264(1), 217( 1), 134(29),111(36),93(100),91(72),69(14),55(43),43(30).
He HH,!I,. 8: ElMS, 70 eV, m/z (% reI. int): 172(1),134(9), 119(12),92(49),91(100),83(22),55(8),39(3).
,l],JlH npe,!l,BapHTeJlbHOH ou.eHKH aKTHBHOCTH 3cpHpHoro MacJlaA. kasakorum HCnOJ1b30BaJlH MeTO,!l, MHKpOpa3Be,!l,eHH5I [4]. 3cpHpHoe MaCJlO paCTBOp5IJ1H B ,l],MCO, cepHIO pa3BeJ],eHHH 3cpHpHoro MaCJla flpOH3BO)J.HJlH B CTepHJlbHOH ,!I,HCTHnJlHpOBaHHoH BO,!l,eB 96-H'leHCTOH MHKpOTInpOBaJlbHOH nJlaWKe,!l,O 0.97 MKr/MJI. CBe)((eBblpaIlI.eHHble 6aKTepHaJlbHbIe cycneH3HH Mueller-Hinton 6YJlbOHa B )J.BOHHOH KOHIJ.eHTpau.HH H )J.pO)(()((eBYIO cycfleH3HIO Candida albicans npHBO,!l,HJlH K KOHueHTpaUHl1 108 CFU/MJ1 (MacFarland No: 0.5). B KaIfeCTBe KOHTpOJ1H pOCTa HCnOJlb30BaJlH CTepHJlbHYIO ,!I,HCTHJ1J1HpOBaHHYJO BO,!l,Y, B Ka)((,!I,YIO HIfeHKY ,!I,06aBJlHJlH flO 100 MKJ1 MHKpo6HOH cycneH3HH. nOCJle,!l,HHH pH,!I" cO,!l,ep)((aw,HH JlHllIb cepHHHble pa3Be,neHH5I
aHTHMHKpo6Horo areHTa 6e3 MHKpoopraHH3Ma,
HCnOJlb30BaJlH KaK HeranlBHbIH KOHTponb. nOCJle HHry6aIJ.HH npH 37°C B TeIfeHHe 24 If nepBYIO 5I'leHKY 6e3 npHpOCTa onpe)J.eJlHJlH KaK MHHHMaJlbHYIO HHrH6HPYIOW,YIO
KOHIJ.eHTpaUHIO (MI1K). ComaCHO Ta6J1. 2, aKTHBHOCTb 3cpHpHoro MaCJla A. kasakorum cpaBHHMa C aKTHBHOCTbIO npenapaToB cpaBHeHHH XJIOpaMcpeHHKOJIacYKIJ.HHaTOM (.D.JIJI 6aKTepHH) H KeTOKOHa30J10M (.D.JI5I ,np°)I{)KeH)flpOTHBWTaMMOB
Escherichia coli, Pseudomonas aeruginosa H Candida albicans.
TABJU1I.JA 2. AHTHMHKpo6HaH aKTHBHOCTb 31j1HpHoro MaCJla
A. kasakorum
] MHK !
62.5 125 250 250 125 125 125
CT 62.5 7.81 250 62.5 31.25
62.5 125-Kero
MIIKpoopraHII3MEscherichia coli, Gr( -) Staphylococcus aureus, Gr(+) Pseudomonas aeruginosa, Gr( -) Enterobacter aerogenes, Gr( -) Proteus vulgaris, Gr(-) Salmonella typhimirium, Gr( -) Candida albicans, LlpOJlOKII
HCTO'lHIIK A TCC 25922
ATCC 6538 ATCC 27853 NRRL 3567 NRRL 123 NRRL 4420 a.G.UTi£. Fac.
MI1K -
MHHHMaJlbHa5I HHrH6HPYJOlUa5I KOHIJ.eHTpaIJ.HJI3cpHpHorO MaCJIa nOJ1bIHHKa3aXCKOH;
CT .- MI1K CTaH,!I,apTHblx npenapaTOB CpaBHeHHSI -
XJ10paMcpeHHKOJIa cYKlI,HHaTa (,!I,J1H6aKTepHH), KeTOKOHa30JIa (AJ1H,!I,pO)l{)I{eH).
TaKHM 06pa30M, B pe3YJ1bTaTe npOBe,!l,eHHOrO HCCJIe,!l,OBaHlUI Onpe,!l,eJIeH KOMnOHeHTHbIi1 COCTaB 3cpHpHoro MaCJ1a nOJIbIHH Ka3axCKOH, OCHOBHbIM KOMnOHeHTOM KOTOpOro 5IBJ15IeTC5ICa6HHHJIaueTaT.
113yqeHHe aHTHMHKpo6HOH aKTHBHOCTH 3cpHpHoro Macna nOJ1bIHH Ka3aXCKOH nOKa3aJ10 era YMepeHHO BbIpa)((eHHOe ,!I,eHCTBI1e B OTHOllIeHHH rpaMOTpHuaTeJ1bHbIX WTaMMOB.
JU1TEPATYPA
1. (/JJlOpa KG3a.xcmGl-IG, no,i:\ pe,l\. H.B. naBJIOBa, AJ1Ma-ATa, 8, 1965
