Thus, this work is essentially postponed and will not be carried out directly in 2012 pending the results of optimization of the germplasm conservation activities and application of the standard operating procedures in the genebank. The immediate viability test of 4,820 accessions provided information on the status of rice germplasm stored in the cold storage facility of the PhilRice Genebank. Evaluation of the grain quality of germplasm as well as the important biotic and abiotic stresses of rice is central to preparing the genetic resources for use in research and breeding of rice varieties.
This activity is critical and important to establish the current status (physical location of the seeds and database information) of the rice germplasm. The main tasks in the conservation of rice germplasm are the registration and processing of new acquisitions, low temperature drying of seeds, initial viability tests and monitoring, and packaging for medium and long-term storage. Incoming seeds with viability scores below 85% are set for prime season seed regeneration.
The seeds for each collection are stored in the base (long-term) pool and the active pool (or distribution). 515 and 545 collections of dry and wet season rice germplasm were selected, respectively, and planted in the field and screen for regeneration (Table 3). The data obtained can serve as a basis when planning the regeneration of rice germplasm in the next growing season to achieve sufficient higher harvests.
Also, the online database which will provide basic and other important data related to the accession of germplasm to the PhilRice Genebank for all interested parties, is being completed and improved.
Evaluation of PhilRice Germplasm Collection for Grain Quality and Tolerance to Biotic and Abiotic Stresses
Panangtingiting ti PhilRice germplasm collection para iti kalidad ti bukbukel ken panaganus kadagiti biotic ken abiotic stresses. Dagiti sungbat ti PhilRice germplasm rice accessions iti blast, induce ken BPH/GLH iti sidong dagiti kondision ti balay ti iskrin (2012WS). Panangtingiting ti PhilRice germplasm collection para iti kalidad ti bukbukel.
Information on the morphological and agronomic characteristics, the response to biotic and abiotic stresses and the grain quality characteristics contribute to the importance of the germplasm and their potential for ultimate use by breeders and other stakeholders. Good grain quality determines the consumer acceptability and marketability of rice, and is therefore considered an important part of the rice breeding program. Evaluation and characterization of the PhilRice germplasm collection for grain quality is therefore important for effective use and successful conservation.
This study aims to evaluate the grain quality of the PhilRice germplasm collection as specified in the Descriptors for Rice published by Bioversity International and effectively provide grain quality data through a computerized database system. Most of the accessions had a milled rice recovery of at least 65%, indicating good milling recovery. The majority of accessions from each batch had grain dimensions of long and slender with a grain length and shape (L:W ratio) of at least 6.4 mm and >3.0, respectively.
21 accessions from batch 1, 7 accessions from batch 2 and 24 accessions from batch 3 were pigmented and/or sticky (with opaque endosperm). Most accessions had an amylose content of 12–25%, which is low to medium in classification, while 29 and 84 of 425 accessions from batches 1–3 were identified as waxy and high, respectively. Of the 47 accessions with high AC from batch 1, 11 had medium gel consistency, while 36 had hard gel consistency.
By aroma detection using the KOH sniffing method, 3 analysts scored 54 of the 215 accessions from batch 1 as aromatic. Of the 425 accessions with full grain quality traits from batches 1-3, 51 were identified with generally good grain quality. These accessions had superior head rice yield (grade 1 to premium), chalkiness of less than 5%, long and slender grain dimensions, and an apparently soft to medium cooked rice texture [14.2 to 24.4% amylose content and a medium to low gelatinization temperature ( <74.0°C)].
Seed Quality Testing of Rice Germplasm Susan R. Brena
Only 792 accessions in the total active collections (2,212) stored in the drying room and tested for viability had a viability of 85–100%. Accessions with low viability should be prioritized in the regeneration cycle to be carried out. In addition, the same additions must be checked in the base collection, because the base collection should be the one used in the regeneration.
The core collection of these accessions in the active collection should be generated as soon as possible to maintain a good number of samples in the gene bank collection. This study aims to determine the integrity of the seed files used as a reference standard for germplasm verification in the PhilRice Genebank. MIC Calayugan, TE Mananghaya, VG Dalusong and LM Perez In the development of rice cultivars adapted to specific environmental conditions, genetic resources are important in providing suitable parental donors that can be sources of traits or genes important for yield. high, pest and disease resistance and grain quality.
As part of the preliminary investigation, the focus is to determine potential founder lines in the PhilRice genetic resources. Decisions on founder lines to be included in the MAGIC population will be made with the breeders and considering unique agronomic traits suited to the needs of Philippine rice farmers. Review the strategies carried out in the papers "Population Development by Multi-Parent Advanced Generation Intercrosses (MAGIC) Among Diverse Genotypes to Facilitate Gene Discovery for Various Traits in Rice" and "Development of Multi-Parent Advanced Generation Intercross (MAGIC) Populations using diverse genotypes to facilitate gene discovery for multiple traits in rice (Oryza.
In normal gene bank operations, accessions are obtained and processed by following standard operating procedures, including the preparation of original seed files. In rice, Yashitola et al. 2002) suggested that a single, appropriately chosen SSR marker is sufficient to assess the purity of hybrid seeds in the F1 hybrid and parental lines. This study focused on comparing the DNA fingerprints of the original seed files and the conserved germplasm accessions contained in both active and basic collections.
In recent seed preparation for germplasm regeneration and characterization in 2012 DS, some seed. Although these accessions were not included for the 2012DS, the probability of getting the same scenario in the future remains. Therefore, there is a need to establish the similarity of seed files and those stored in active collections.
96 germplasm accessions were randomly identified (Table 15) for comparison of microsatellite DNA fingerprints of both seed files and active collections. Germplasm utilities used to compare microsatellite DNA fingerprints of seed files and active collections.
