RESULTS
4.4 Polymerase chain reaction-restriction fragment length polymorphisms (PCR- RFLP) for miR 146a (G>C) polymorphism in controls
4.4 Polymerase chain reaction-restriction fragment length polymorphisms (PCR-
Figure 4.15 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 56/315 is PCR product in controls and genotype is C/G Lane 3, 56/351 is PCR product in controls and genotype is C/C Lane 4, 56/470 is PCR product in controls and genotype is G/G Lane 5, 56/501 is PCR product in controls and genotype is C/G Lane 6, 56/503 is PCR product in controls and genotype is C/C Lane 7, 56/540 is PCR product in controls and genotype is C/G Lane 8, 56/597 is PCR product in controls and genotype is C/G Lane 9, 56/717 is PCR product in controls and genotype is C/G Lane10,56/738 is PCR product in controls and genotype is C/G
122 bp 147 bp
Figure 4.16 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 56/496 is PCR product in controls and genotype is C/G Lane 3, 56/523 is PCR product in controls and genotype is C/G Lane 4, 56/739 is PCR product in controls and genotype is G/G Lane 5, 56/741 is PCR product in controls and genotype is C/G Lane 6, 56/762 is PCR product in controls and genotype is C/G Lane 7, 56/779 is PCR product in controls and genotype is C/G Lane 8, 56/811 is PCR product in controls and genotype is G/G Lane 9, 56/846 is PCR product in controls and genotype is C/G Lane10,56/922 is PCR product in controls and genotype is C/G
122 bp 147 bp
Figure 4.17 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 57/739 is PCR product in controls and genotype is C/C Lane 3, 57/769 is PCR product in controls and genotype is C/C Lane 4, 57/900 is PCR product in controls and genotype is C/C Lane 5, 57/859 is PCR product in controls and genotype is C/G Lane 6, 56/23 is PCR product in controls and genotype is G/G Lane 7, 56/95 is PCR product in controls and genotype is C/C Lane 8, 56/96 is PCR product in controls and genotype is C/G Lane 9, 56/148 is PCR product in controls and genotype is C/C Lane10,56/248 is PCR product in controls and genotype is C/G
122 bp 147 bp
Figure 4.18 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 58/492 is PCR product in controls and genotype is C/C Lane 3, 57/40 is PCR product in controls and genotype is C/G Lane 4, 57/100 is PCR product in controls and genotype is G/G Lane 5, 57/117 is PCR product in controls and genotype is C/G Lane 6, 57/155 is PCR product in controls and genotype is C/G Lane 7, 57/176 is PCR product in controls and genotype is C/C Lane 8, 57/193 is PCR product in controls and genotype is C/G Lane 9, 57/324 is PCR product in controls and genotype is C/G Lane10,57/353 is PCR product in controls and genotype is C/C
122 bp 147 bp
Figure 4.19 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 57/33 is PCR product in controls and genotype is C/C Lane 3, 57/77 is PCR product in controls and genotype is G/G Lane 4, 57/116 is PCR product in controls and genotype is G/G Lane 5, 57/215 is PCR product in controls and genotype is C/C Lane 6, 57/351 is PCR product in controls and genotype is G/G Lane 7, 57/360 is PCR product in controls and genotype is C/G Lane 8, 57/655 is PCR product in controls and genotype is C/C Lane 9, 57/687 is PCR product in controls and genotype is C/G Lane10,57/701 is PCR product in controls and genotype is C/G
122 bp 147 bp
Figure 4.20 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 55/810 is PCR product in controls and genotype is C/G Lane 3, 56/883 is PCR product in controls and genotype is G/G Lane 4, 56/890 is PCR product in controls and genotype is C/C Lane 5, 56/903 is PCR product in controls and genotype is G/G Lane 6, 56/931 is PCR product in controls and genotype is C/C Lane 7, 56/996 is PCR product in controls and genotype is C/G Lane 8, 58/335 is PCR product in controls and genotype is C/G
122 bp 147 bp
Figure 4.21 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 56/34 is PCR product in controls and genotype is C/C Lane 3, 57/558 is PCR product in controls and genotype is C/G Lane 4, 57/580 is PCR product in controls and genotype is C/C Lane 5, 56/41 is PCR product in controls and genotype is C/G Lane 6, 57/382 is PCR product in controls and genotype is C/C Lane 7, 57/373 is PCR product in controls and genotype is C/G Lane 8, 57/829 is PCR product in controls and genotype is C/C Lane 9, 57/735 is PCR product in controls and genotype is C/C Lane10,57/834 is PCR product in controls and genotype is C/C
122 bp 147 bp
Figure 4.22 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 57/434 is PCR product in controls and genotype is G/G Lane 3, 57/454 is PCR product in controls and genotype is C/G Lane 4, 57/489 is PCR product in controls and genotype is C/C Lane 5, 57/497 is PCR product in controls and genotype is C/C Lane 6, 57/526 is PCR product in controls and genotype is G/G Lane 7, 57/535 is PCR product in controls and genotype is C/G Lane 8, 57/536 is PCR product in controls and genotype is G/G Lane 9, 57/537 is PCR product in controls and genotype is C/C Lane10,57/721 is PCR product in controls and genotype is G/G
122 bp 147 bp
Figure 4.23 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 57/174 is PCR product in controls and genotype is C/C Lane 3, 57/173 is PCR product in controls and genotype is C/C Lane 4, 57/204 is PCR product in controls and genotype is C/C Lane 5, 57/213 is PCR product in controls and genotype is C/C Lane 6, 57/232 is PCR product in controls and genotype is G/G Lane 7, 57/241 is PCR product in controls and genotype is C/G Lane 8, 57/280 is PCR product in controls and genotype is C/G Lane 9, 57/282 is PCR product in controls and genotype is C/G
122 bp 147 bp
Figure 4.24 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 57/47 is PCR product in controls and genotype is C/G Lane 3, 58/696 is PCR product in controls and genotype is C/C Lane 4, 57/115 is PCR product in controls and genotype is C/G Lane 5, 57/36 is PCR product in controls and genotype is C/G Lane 6, 57/75 is PCR product in controls and genotype is C/G Lane 7, 57/90 is PCR product in controls and genotype is C/C Lane 8, 57/68 is PCR product in controls and genotype is C/G Lane 9, 57/70 is PCR product in controls and genotype is C/G Lane10,57/74 is PCR product in controls and genotype is C/G
122 bp 147 bp
Figure 4.25 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 57/81 is PCR product in controls and genotype is C/C Lane 3, 57/35 is PCR product in controls and genotype is C/G Lane 4, 57/101 is PCR product in controls and genotype is C/G Lane 5, 57/120 is PCR product in controls and genotype is C/G Lane 6, 57/121 is PCR product in controls and genotype is C/G Lane 7, 57/144 is PCR product in controls and genotype is G/G Lane 8, 57/151 is PCR product in controls and genotype is C/G Lane 9, 57/156 is PCR product in controls and genotype is C/C Lane10,57/166 is PCR product in controls and genotype is C/C
122 bp 147 bp
Figure 4.26 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 57/340 is PCR product in controls and genotype is C/G Lane 3, 57/339 is PCR product in controls and genotype is G/G Lane 4, 57/879 is PCR product in controls and genotype is C/G Lane 5, 56/26 is PCR product in controls and genotype is G/G Lane 6, 57/501 is PCR product in controls and genotype is C/G Lane 7, 57/597 is PCR product in controls and genotype is C/G Lane 8, 57/371 is PCR product in controls and genotype is C/G Lane 9, 57/406 is PCR product in controls and genotype is C/C Lane10,57/309 is PCR product in controls and genotype is C/C
122 bp 147 bp
Figure 4.27 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 57/570 is PCR product in controls and genotype is C/C Lane 3, 57/714 is PCR product in controls and genotype is C/G Lane 4, 57/903 is PCR product in controls and genotype is C/C Lane 5, 57/483 is PCR product in controls and genotype is G/G Lane 6, 57/830 is PCR product in controls and genotype is C/C Lane 7, 57/787 is PCR product in controls and genotype is C/G Lane 8, 57/706 is PCR product in controls and genotype is C/G Lane 9, 57/885 is PCR product in controls and genotype is C/C Lane10,57/349 is PCR product in controls and genotype is C/G
122 bp 147 bp
Figure 4.28 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 57/559 is PCR product in controls and genotype is C/C Lane 3, 57/356 is PCR product in controls and genotype is C/C Lane 4, 57/405 is PCR product in controls and genotype is C/G Lane 5, 57/317 is PCR product in controls and genotype is C/G Lane 6, 57/555 is PCR product in controls and genotype is G/G Lane 7, 57/629 is PCR product in controls and genotype is C/G Lane 8, 57/389 is PCR product in controls and genotype is C/C Lane 9, 57/411 is PCR product in controls and genotype is C/C Lane10,57/456 is PCR product in controls and genotype is C/C
122 bp 147 bp
Figure 4.29 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 57/286 is PCR product in controls and genotype is C/C Lane 3, 58/678 is PCR product in controls and genotype is C/G Lane 4, 58/655 is PCR product in controls and genotype is C/G Lane 5, 58/670 is PCR product in controls and genotype is C/C Lane 6, 58/664 is PCR product in controls and genotype is G/G Lane 7, 58/700 is PCR product in controls and genotype is C/C Lane 8, 58/318 is PCR product in controls and genotype is C/G Lane 9, 58/341 is PCR product in controls and genotype is C/G Lane10,57/318 is PCR product in controls and genotype is C/G
122 bp 147 bp
Figure 4.30 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 58/22 is PCR product in controls and genotype is C/C Lane 3, 58/106 is PCR product in controls and genotype is C/G Lane 4, 58/128 is PCR product in controls and genotype is C/G Lane 5, 58/148 is PCR product in controls and genotype is C/G Lane 6, 58/161 is PCR product in controls and genotype is C/C Lane 7, 58/289 is PCR product in controls and genotype is C/G Lane 8, 58/389 is PCR product in controls and genotype is C/G Lane 9, 55/26 is PCR product in controls and genotype is C/G Lane10,55/29 is PCR product in controls and genotype is G/G
122 bp 147 bp
Figure 4.31 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 58/142 is PCR product in controls and genotype is C/C Lane 3, 58/144 is PCR product in controls and genotype is C/C Lane 4, 58/174 is PCR product in controls and genotype is C/G Lane 5, 58/106 is PCR product in controls and genotype is C/G Lane 6, 58/5 is PCR product in controls and genotype is C/G Lane 7, 58/55 is PCR product in controls and genotype is G/G Lane 8, 58/223 is PCR product in controls and genotype is C/G Lane 9, 58/233 is PCR product in controls and genotype is G/G Lane10,58/260 is PCR product in controls and genotype is C/C
122 bp 147 bp
Figure 4.32 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 58/249 is PCR product in controls and genotype is C/C Lane 3, 58/28 is PCR product in controls and genotype is C/C Lane 4, 58/44 is PCR product in controls and genotype is C/G Lane 5, 58/45 is PCR product in controls and genotype is C/C Lane 6, 58/381 is PCR product in controls and genotype is C/C Lane 7, 58/80 is PCR product in controls and genotype is C/C Lane 8, 58/202 is PCR product in controls and genotype is C/G Lane 9, 58/375 is PCR product in controls and genotype is C/G Lane10,58/207 is PCR product in controls and genotype is C/G
122 bp 147 bp
Figure 4.33 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 58/563 is PCR product in controls and genotype is G/G Lane 3, 58/580 is PCR product in controls and genotype is C/C Lane 4, 58/525 is PCR product in controls and genotype is C/G Lane 5, 58/602 is PCR product in controls and genotype is C/C Lane 6, 58/612 is PCR product in controls and genotype is C/C Lane 7, 58/637 is PCR product in controls and genotype is G/G Lane 8, 58/648 is PCR product in controls and genotype is C/G Lane 9, 58/653 is PCR product in controls and genotype is C/C
122 bp 147 bp
Figure 4.34 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 58/248 is PCR product in controls and genotype is C/G Lane 3, 58/290 is PCR product in controls and genotype is G/G Lane 4, 58/373 is PCR product in controls and genotype is C/C Lane 5, 58/386 is PCR product in controls and genotype is C/C Lane 6, 58/390 is PCR product in controls and genotype is C/G Lane 7, 58/410 is PCR product in controls and genotype is C/G Lane 8, 58/432 is PCR product in controls and genotype is C/G Lane 9, 58/451 is PCR product in controls and genotype is C/G Lane10,58/469 is PCR product in controls and genotype is C/G
122 bp 147 bp
Figure 4.35 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 58/301 is PCR product in controls and genotype is G/G Lane 3, 58/364 is PCR product in controls and genotype is C/G Lane 4, 58/404 is PCR product in controls and genotype is C/G Lane 5, 58/431 is PCR product in controls and genotype is G/G Lane 6, 58/444 is PCR product in controls and genotype is C/C Lane 7, 58/477 is PCR product in controls and genotype is C/C Lane 8, 58/491 is PCR product in controls and genotype is C/C Lane 9, 58/523 is PCR product in controls and genotype is C/C Lane10,58/535 is PCR product in controls and genotype is G/G
122 bp 147 bp
Figure 4.36 Demonstrate PCR products after restriction in controls.
Lane 1, M is 50 base pair marker
Lane 2, 58/276 is PCR product in controls and genotype is C/G Lane 3, 58/287 is PCR product in controls and genotype is C/G Lane 4, 58/292 is PCR product in controls and genotype is G/G Lane 5, 58/304 is PCR product in controls and genotype is C/G Lane 6, 58/311 is PCR product in controls and genotype is C/G Lane 7, 58/328 is PCR product in controls and genotype is C/G Lane 8, 58/327 is PCR product in controls and genotype is G/G Lane 9, 58/334 is PCR product in controls and genotype is C/G Lane10,58/340 is PCR product in controls and genotype is C/G
122 bp 147 bp