RESULTS
4.3 Polymerase chain reaction-restriction fragment length polymorphisms (PCR- RFLP) for miR 146a (G>C) polymorphism in ALL patients
Figure 4.3 Demonstrate PCR products after restriction in ALL patients.
Lane 1, M is 50 base pair marker
Lane 2, ALL 2 is PCR product in ALL patients and genotype is G/G Lane 3, ALL 3 is PCR product in ALL patients and genotype is G/G Lane 4, ALL 4 is PCR product in ALL patients and genotype is G/G Lane 5, ALL 5 is PCR product in ALL patients and genotype is C/G Lane 6, ALL 6 is PCR product in ALL patients and genotype is C/G Lane 7, ALL 7 is PCR product in ALL patients and genotype is C/G Lane 8, ALL 8 is PCR product in ALL patients and genotype is C/C Lane 9, ALL 9 is PCR product in ALL patients and genotype is C/C Lane10, ALL 11 is PCR product in ALL patients and genotype is C/C
147 bp 122 bp
Figure 4.4 Demonstrate PCR products after restriction in ALL patients.
Lane 1, M is 50 base pair marker
Lane 2, ALL 13 is PCR product in ALL patients and genotype is G/G Lane 3, ALL 15 is PCR product in ALL patients and genotype is C/G Lane 4, ALL 18 is PCR product in ALL patients and genotype is C/C Lane 5, ALL 19 is PCR product in ALL patients and genotype is C/G Lane 6, ALL 20 is PCR product in ALL patients and genotype is C/C Lane 7, ALL 21 is PCR product in ALL patients and genotype is G/G Lane 8, ALL 22 is PCR product in ALL patients and genotype is C/C Lane 9, ALL 23 is PCR product in ALL patients and genotype is C/G Lane10,ALL 24 is PCR product in ALL patients and genotype is C/G
147 bp 122 bp
Figure 4.5 Demonstrate PCR products after restriction in ALL patients.
Lane 1, M is 50 base pair marker
Lane 2, ALL 26 is PCR product in ALL patients and genotype is C/G Lane 3, ALL 27 is PCR product in ALL patients and genotype is C/G Lane 4, ALL 28 is PCR product in ALL patients and genotype is C/C Lane 5, ALL 29 is PCR product in ALL patients and genotype is G/G Lane 6, ALL 30 is PCR product in ALL patients and genotype is C/G Lane 7, ALL 31 is PCR product in ALL patients and genotype is G/G Lane 8, ALL 32 is PCR product in ALL patients and genotype is C/G Lane 9, ALL 33 is PCR product in ALL patients and genotype is C/C Lane10, ALL 34 is PCR product in ALL patients and genotype is C/G
122 bp 147 bp
Figure 4.6 Demonstrate PCR products after restriction in ALL patients.
Lane 1, M is 50 base pair marker
Lane 2, ALL 36 is PCR product in ALL patients and genotype is C/G Lane 3, ALL 38 is PCR product in ALL patients and genotype is C/C Lane 4, ALL 40 is PCR product in ALL patients and genotype is C/G Lane 5, ALL 41 is PCR product in ALL patients and genotype is C/C Lane 6, ALL 43 is PCR product in ALL patients and genotype is C/C Lane 7, ALL 44 is PCR product in ALL patients and genotype is C/G Lane 8, ALL 46 is PCR product in ALL patients and genotype is C/G Lane 9, ALL 47 is PCR product in ALL patients and genotype is C/G Lane10,ALL 48 is PCR product in ALL patients and genotype is C/G
122 bp 147 bp
Figure 4.7 Demonstrate PCR products after restriction in ALL patients.
Lane 1, M is 50 base pair marker
Lane 2, ALL 50 is PCR product in ALL patients and genotype is C/C Lane 3, ALL 53 is PCR product in ALL patients and genotype is C/C Lane 4, ALL 56 is PCR product in ALL patients and genotype is C/C Lane 5, ALL 57 is PCR product in ALL patients and genotype is C/G Lane 6, ALL 58 is PCR product in ALL patients and genotype is C/G Lane 7, ALL 62 is PCR product in ALL patients and genotype is C/C Lane 8, ALL 63 is PCR product in ALL patients and genotype is C/C Lane 9, ALL 64 is PCR product in ALL patients and genotype is C/C Lane10, ALL 65 is PCR product in ALL patients and genotype is C/C
122 bp 147 bp
Figure 4.8 Demonstrate PCR products after restriction in ALL patients.
Lane 1, M is 50 base pair marker
Lane 2, ALL 57/574 is PCR product in ALL patients and genotype is C/C Lane 3, ALL 58/589 is PCR product in ALL patients and genotype is C/G Lane 4, ALL 48 is PCR product in ALL patients and genotype is C/G Lane 5, ALL 49 is PCR product in ALL patients and genotype is C/G Lane 6, ALL 55 is PCR product in ALL patients and genotype is C/C Lane 7, ALL 66 is PCR product in ALL patients and genotype is C/C Lane 8, ALL 68 is PCR product in ALL patients and genotype is C/G Lane 9, ALL 69 is PCR product in ALL patients and genotype is G/G Lane10,ALL 70 is PCR product in ALL patients and genotype is G/G
122 bp 147 bp
Figure 4.9 Demonstrate PCR products after restriction in ALL patients.
Lane 1, M is 50 base pair marker
Lane 2, ALL 71 is PCR product in ALL patients and genotype is C/G Lane 3, ALL 72 is PCR product in ALL patients and genotype is C/G Lane 4, ALL 73 is PCR product in ALL patients and genotype is C/C Lane 5, ALL 74 is PCR product in ALL patients and genotype is C/C Lane 6, ALL 75 is PCR product in ALL patients and genotype is C/G Lane 7, ALL 77 is PCR product in ALL patients and genotype is C/G Lane 8, ALL 78 is PCR product in ALL patients and genotype is C/C Lane 9, ALL 80 is PCR product in ALL patients and genotype is C/G Lane10, ALL 97 is PCR product in ALL patients and genotype is C/G
122 bp 147 bp
Figure 4.10 Demonstrate PCR products after restriction in ALL patients.
Lane 1, M is 50 base pair marker
Lane 2, ALL 98 is PCR product in ALL patients and genotype is C/C Lane 3, ALL 99 is PCR product in ALL patients and genotype is C/G Lane 4, ALL 100 is PCR product in ALL patients and genotype is C/C Lane 5, ALL 101 is PCR product in ALL patients and genotype is C/C Lane 6, ALL 102 is PCR product in ALL patients and genotype is C/C Lane 7, ALL 103 is PCR product in ALL patients and genotype is C/C Lane 8, ALL 104 is PCR product in ALL patients and genotype is C/G Lane 9, ALL 105 is PCR product in ALL patients and genotype is C/G Lane10,ALL 106 is PCR product in ALL patients and genotype is C/C
122 bp 147 bp
Figure 4.11 Demonstrate PCR products after restriction in ALL patients.
Lane 1, M is 50 base pair marker
Lane 2, ALL 107 is PCR product in ALL patients and genotype is C/G Lane 3, ALL 108 is PCR product in ALL patients and genotype is C/C Lane 4, ALL 109 is PCR product in ALL patients and genotype is C/G Lane 5, ALL 110 is PCR product in ALL patients and genotype is C/G Lane 6, ALL 111 is PCR product in ALL patients and genotype is C/C Lane 7, ALL 112 is PCR product in ALL patients and genotype is G/G Lane 8, ALL 113 is PCR product in ALL patients and genotype is C/G Lane 9, ALL 114 is PCR product in ALL patients and genotype is C/C Lane10,ALL 115 is PCR product in ALL patients and genotype is G/G
122 bp 147 bp
Figure 4.12 Demonstrate PCR products after restriction in ALL patients.
Lane 1, M is 50 base pair marker
Lane 2, ALL 117 is PCR product in ALL patients and genotype is C/C Lane 3, ALL 118 is PCR product in ALL patients and genotype is C/G Lane 4, ALL 119 is PCR product in ALL patients and genotype is C/C Lane 5, ALL 120 is PCR product in ALL patients and genotype is C/G Lane 6, ALL 121 is PCR product in ALL patients and genotype is C/G Lane 7, ALL 122 is PCR product in ALL patients and genotype is C/C Lane 8, ALL 123 is PCR product in ALL patients and genotype is C/G Lane 9, ALL 124 is PCR product in ALL patients and genotype is C/G Lane10,ALL 125 is PCR product in ALL patients and genotype is C/G
122 bp 147 bp
Figure 4.13 Demonstrate PCR products after restriction in ALL patients.
Lane 1, M is 50 base pair marker
Lane 2, ALL 126 is PCR product in ALL patients and genotype is C/G Lane 3, ALL 127 is PCR product in ALL patients and genotype is C/C Lane 4, ALL 58/317 is PCR product in ALL patients and genotype is C/G Lane 5, ALL 58/399 is PCR product in ALL patients and genotype is C/C Lane 6, ALL 58/467 is PCR product in ALL patients and genotype is C/C Lane 7, ALL 58/515 is PCR product in ALL patients and genotype is C/G Lane 8, ALL 58/516 is PCR product in ALL patients and genotype is C/G Lane 9, ALL 58/517 is PCR product in ALL patients and genotype is C/G
122 bp 147 bp
4.4 Polymerase chain reaction-restriction fragment length polymorphisms (PCR-