Calibration Curves of Spirillum species strain NOX grown in varied concentration of sodium acetate-C using both CFU

(1)

Calibration Curves of Spirillum species strain NOX grown in varied concentration of sodium acetate-C using both CFU

and ATP measurements

碳源: CH3

COONa

醋酸鈉濃度: 0,10,50,100,150,200 ug/L

水源: 學校實驗用

RO

水?????Source water drown from deep well was pretreated

with ??????? . and then treated by ?????? ; The NPDOC values was 0.764 mg/L..[This value looks higher than other literatures cited. Do you have measure the molecular weight ?????]

培養體積: 300mL

圖

1

為

NOX 菌種在不同醋酸鈉添加濃度下之生長曲線圖，由圖可知第 0

到

5

天屬對數生長期，不同醋酸鈉濃度下

NOX

皆快速增長，第

5

天後低醋酸鈉濃度開始進入平穩期，高醋酸鈉濃度則在第

8

或

10

天達到平穩期，無添加醋酸鈉時

NOX

僅利用原水中的有機質(NPDOC為

0.7 mg/L)，因此菌數較其他有添加

醋酸鈉者為低。

另將生長曲線之最大菌落數與個別對應之

acetate-C 濃度，繪製圖 2

並求出

NOX 之產率，相關研究之產率整理如表 1

所示，由表中可發現本研究所得產率

值與

van der kooij et al. (1995)之研究結果值均屬視為在同級次方(order)範圍，表

示實驗之 QA/QC 應是值得信賴的。

本研究也利用

ATP

測定作為輔助，來觀察不同醋酸鈉濃度添加下

NOX

生長曲線，取生長曲線之

ATP

最大值與對應的醋酸鈉濃度做率定曲線，結果顯示如圖

3，r

值為

0.9923 顯示此方法為可行。

(2)

incubation time (days)

0 2 3 5 6 8 10 12

co lo ny c ou nt ( C F U /m L )

1e+2 1e+3 1e+4 1e+5 1e+6 1e+7 1e+8

0 10 50 100 150 200 acetate concentration (ug acetate-C/L)

圖

1 Spirillum species strain NOX 於各種 acetate-C 濃度下之生長曲線[insignificant difference between 200

與

150 g acetate-C/L]

strain NOX

acetate concentration (ug acetate-C/L)

0 20 40 60 80 100 120 140 160

m ax im um c ol on y co u nt ( C F U /m L )

0 1e+6 2e+6 3e+6 4e+6 5e+6 6e+6 7e+6

r=0.9971

yield=3.82*10

⁷

CFU/ug acetate-C

圖

2 Spirillum species strain NOX

之最大菌落數與

acetate-C 之產率關係

(3)

(larger circle should be modified)

In this study, the growth yield of NOX, 3.82 CFU/µg acetate-C .was calculated by CFU versus sodium acetate-C. Compared with those values of literatures, same order was found in this experiment. It proved that the selection of source water , the procedure of experiment, and environment condition in our laboratory

表

1 相關研究 Spirillum species strain NOX

之產率

菌種

Van der kooij et al.(1995)

賴氏(2000) 鄭氏(2008) 陳氏(2012)

CFU/µg acetate-C

NO X

1.210

⁷

3.510

⁷

1.5310

⁷

1.110

⁷

acetate concentration (ug acetate-C/L)

0 20 40 60 80 100 120 140 160

R L U / s ec

0.0 2.0e+4 4.0e+4 6.0e+4 8.0e+4 1.0e+5 1.2e+5 1.4e+5

r=0.9923

圖

3 各種 acetate-C 濃度與 ATP

值之率定曲線

(4)

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0

3 0 0 3 5 0 4 0 0 4 5 0 5 0 0 5 5 0

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0

3 0 0 3 5 0 4 0 0 4 5 0 5 0 0 5 5 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0

0 1 0 0

1 0 1 5 0

5 0 2 0 0

E m i s s i o n ( n m ) E m i s s i o n ( n m )

E xc it at io n ( n m ) E xc it at io n ( n m ) E xc it at io n ( n m )

不同醋酸鈉濃度菌數最大值之

EEFM

圖

(5)

acetate concentration (ug acetate-C/L)

A ve ra ge f lu or es ce nc e in te ns it y (% )

0 20 40 60 80 100

I II III IV V

T ot al A F I

0 500 1000 2000

0 10 50 100 150 200

不同醋酸鈉濃度菌數最大值之

AFI

(6)

(7)

(8)

不同醋酸鈉濃度菌數最大值天數

UV200-400

斜率

(9)

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

3 0 0 3 5 0 4 0 0 4 5 0 5 0 0 5 5 0

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

3 0 0 3 5 0 4 0 0 4 5 0 5 0 0 5 5 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0 0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0

d a y 0 d a y 6

d a y 8

d a y 1 0

d a y 1 2 d a y 5

d a y 3 d a y 2

E m i s s i o n ( n m ) E m i s s i o n ( n m )

E xc it at io n ( n m ) E xc it at io n ( n m ) E xc it at io n ( n m ) E xc it at io n ( n m )

(10)

圖

5 NOX

在醋酸鈉濃度

10 ug/L

培養不同天數之 EEFM 圖

(11)

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0 1 6 0 0 1 7 0 0 1 8 0 0 1 9 0 0 2 0 0 0

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0 1 6 0 0 1 7 0 0 1 8 0 0 1 9 0 0 2 0 0 0

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0 1 6 0 0 1 7 0 0 1 8 0 0 1 9 0 0 2 0 0 0

3 0 0 3 5 0 4 0 0 4 5 0 5 0 0 5 5 0 2 0 0

2 5 0 3 0 0 3 5 0 4 0 0

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0 1 2 0 0 1 4 0 0 1 6 0 0 1 8 0 0 2 0 0 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0 1 6 0 0 1 7 0 0 1 8 0 0 1 9 0 0 2 0 0 0

3 0 0 3 5 0 4 0 0 4 5 0 5 0 0 5 5 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0 1 6 0 0 1 7 0 0 1 8 0 0 1 9 0 0 2 0 0 0

E m i s s i o n ( n m ) E m i s s i o n ( n m )

E xc it at io n ( n m ) E xc it at io n ( n m ) E xc it at io n ( n m ) E xc it at io n ( n m )

d a y 0 d a y 6

d a y 2 d a y 8

d a y 3 d a y 1 0

d a y 5 d a y 1 2

圖

6 NOX

在醋酸鈉濃度

50 ug/L

(12)

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0 1 2 0 0 1 4 0 0 1 6 0 0 1 8 0 0 2 0 0 0 2 2 0 0 2 4 0 0 2 6 0 0 2 8 0 0 3 0 0 0

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0 1 2 0 0 1 4 0 0 1 6 0 0 1 8 0 0 2 0 0 0 2 2 0 0 2 4 0 0 2 6 0 0 2 8 0 0 3 0 0 0

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0 1 2 0 0 1 4 0 0 1 6 0 0 1 8 0 0 2 0 0 0 2 2 0 0 2 4 0 0 2 6 0 0 2 8 0 0 3 0 0 0

3 0 0 3 5 0 4 0 0 4 5 0 5 0 0 5 5 0

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0 1 2 0 0 1 4 0 0 1 6 0 0 1 8 0 0 2 0 0 0 2 2 0 0 2 4 0 0 2 6 0 0 2 8 0 0 3 0 0 0

3 0 0 3 5 0 4 0 0 4 5 0 5 0 0 5 5 0

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0 1 2 0 0 1 4 0 0 1 6 0 0 1 8 0 0 2 0 0 0 2 2 0 0 2 4 0 0 2 6 0 0 2 8 0 0 3 0 0 0

d a y 0 d a y 6

d a y 8 d a y 2

d a y 3

d a y 5 d a y 1 2

d a y 1 0

E m i s s i o n ( n m ) E m i s s i o n ( n m ) E xc it at io n ( n m ) E xc it at io n ( n m ) E xc it at io n ( n m ) E xc it at io n ( n m )

圖

7 NOX

在醋酸鈉濃度

100 ug/L

(13)

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0 1 2 0 0 1 4 0 0 1 6 0 0 1 8 0 0 2 0 0 0 2 2 0 0 2 4 0 0 2 6 0 0 2 8 0 0 3 0 0 0 3 2 0 0

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0 1 2 0 0 1 4 0 0 1 6 0 0 1 8 0 0 2 0 0 0 2 2 0 0 2 4 0 0 2 6 0 0 2 8 0 0 3 0 0 0 3 2 0 0

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0 1 2 0 0 1 4 0 0 1 6 0 0 1 8 0 0 2 0 0 0 2 2 0 0 2 4 0 0 2 6 0 0 2 8 0 0 3 0 0 0 3 2 0 0

3 0 0 3 5 0 4 0 0 4 5 0 5 0 0 5 5 0

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0 1 2 0 0 1 4 0 0 1 6 0 0 1 8 0 0 2 0 0 0 2 2 0 0 2 4 0 0 2 6 0 0 2 8 0 0 3 0 0 0 3 2 0 0

3 0 0 3 5 0 4 0 0 4 5 0 5 0 0 5 5 0

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0 1 2 0 0 1 4 0 0 1 6 0 0 1 8 0 0 2 0 0 0 2 2 0 0 2 4 0 0 2 6 0 0 2 8 0 0 3 0 0 0 3 2 0 0

d a y 0

d a y 2

d a y 6

d a y 8

d a y 1 0 d a y 3

d a y 1 2 d a y 5

E m i s s i o n ( n m ) E m i s s i o n ( n m )

E xc it at io n ( n m ) E xc it at io n ( n m ) E xc it at io n ( n m ) E xc it at io n ( n m )

(14)

圖

8 NOX

在醋酸鈉濃度

150 ug/L

(15)

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0 1 6 0 0 1 7 0 0 1 8 0 0

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0 1 6 0 0 1 7 0 0 1 8 0 0

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0 1 6 0 0 1 7 0 0 1 8 0 0

3 0 0 3 5 0 4 0 0 4 5 0 5 0 0 5 5 0

2 0 0 2 5 0 3 0 0 3 5 0 4 0 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0 1 6 0 0 1 7 0 0 1 8 0 0

01 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0 1 6 0 0 1 7 0 0 1 8 0 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0 1 6 0 0 1 7 0 0 1 8 0 0

01 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0 1 6 0 0 1 7 0 0 1 8 0 0

3 0 0 3 5 0 4 0 0 4 5 0 5 0 0 5 5 0

0 1 0 0 2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0 1 5 0 0 1 6 0 0 1 7 0 0 1 8 0 0

d a y 0 d a y 6

d a y 2 d a y 8

d a y 1 0 d a y 3

d a y 1 2 d a y 5

E m i s s i o n ( n m ) E m i s s i o n ( n m )

E xc it at io n ( n m ) E xc it at io n ( n m ) E xc it at io n ( n m ) E xc it at io n ( n m )

圖

9 NOX

在醋酸鈉濃度

200 ug/L

(16)

0.0 0.5 1.0 1.5 2.0

0 10 50 100 150 200

0.0 0.5 1.0 1.5 2.0

A bs or b an ce ( cm

-1

) 0.0 0.5 1.0 1.5 2.0

Wavelength (nm)

200 300 400 500 600

0.0 0.5 1.0 1.5 2.0

Wavelength (nm)

200 300 400 500 600

ug acetate-C/L ug acetate-C/L

0 10 50 100 150 200

Day 0 Day 6

Day 2 Day 8

Day 3

Day 5

Day 10

Day 12

A b so rb an ce ( cm

-1

) A bs or b an ce ( cm

-1

) A b so rb an ce ( cm

-1

)

圖

10

不同醋酸鈉濃度及培養天數 UV-VIS吸收光譜圖(原樣)(200-600nm)

(17)

ab so rb an ce ( cm

-1

) 0.0 0.5 1.0 1.5 2.0

0 10 50 100 150 200

ab so rb an ce ( cm

-1

) 0.0 0.5 1.0 1.5 2.0

ab so rb an ce ( cm

-1

) 0.0 0.5 1.0 1.5 2.0

wavelength (nm)

200 220 240 260 280 300 320 340 ab so rb an ce ( cm

-1

)

0.0 0.5 1.0 1.5 2.0

wavelength (nm)

200 220 240 260 280 300 320 340

ug acetate-C/L

Day 0 Day 6

Day 2 Day 8

ug acetate-C/L

ug acetate-C/L ug acetate-C/L

0 10 50 100 150 200

Day 3 Day 10

Day 5 Day 12

ug acetate-C/L 0 10 50 100 150 200 ug acetate-C/L

0 10 50 100 150 200

ug acetate-C/L 0 10 50 100 150 200

圖

11

不同醋酸鈉濃度及培養天數 UV-VIS吸收光譜圖(原樣)(200-350nm)

(18)

ab so rb an ce ( cm

-1

) 0.0 0.2 0.4 0.6 0.8 1.0 1.2

1.4

0

10 50 100 150 200

ab so rb an ce ( cm

-1

) 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4

ab so rb an ce ( cm

-1

) 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4

wavelength (nm)

200 300 400 500 600

ab so rb an ce ( cm

-1

) 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4