KHOA HOC C 6 N G NGHE
PHAIXI L A P , TUYEIM CHOIXI VI KHLIAIXI IXIQI SIMH R i CAY M i A DUdlXIG CO KHA IXIAIXIG Cd DflVH DAIVI V A
TOIXIG HOP IIXIDOLE ACETIC ACID
Nguyen Qudc KhuongS U 'V^nh ThiicS Le Thi My ThuS Urn Tlii Y^n Nhi==, Vo Van ljng3, Tran Chi ^rhan^ Ly Ngpc Thanh XuSn^ v^ NguySn Thi Thanh Xuan^
T6MTAT
Su dung vi Idiuan la mpt trong nhung bi€n phap trien vong giup canh tac mia flirong bSn vung thong qua viec giam thieu luang phan bon hoa hoc. Muc ti&u cua nghien cuu la chon duac nhirng dong vi khuan co dinh dam va tong hop lAA noi sinh r& cay mia duong. Sau mSu r^ mia tr6ng tren dat phen duac thu thap tai huyen Phung Hiep, tinh Hau Giang. Ket qua phan lap dupc 28 dong vi khuan noi sinh rfi cay mia duong tren moi truang NFB, voi 24 dong vi khuan co kha nang song duac trong dieu kien chua. Trong do, nam dong vi khuan co dinh dam tot nhat la KClc. PB3b, KClf, KC2d, KClbl, voi ham lupng 12,1 - 18,5 mg/L va ba dong vi khuan tong hgp lAA tot nhat la HAle, PB2e va KCld, voi ham lupng 12,1 - 29,4 mg/L. Cdn sir dung hon hpp cac dong vi khuan da tuyen chon nham h6 trg sinh truong cay mia, mac du cac dong vi khuan tr^n deu so hiru hai chiic nang.
Tiikh6a: Cd dinh dam, dat phen, tdng bop lAA. mia dudng, vi khudn noi sinh.
I.DATVANDE
Dien tich canh tac mia b ca nuoc c6 xu huong giam, voi dien tich 284,2 nghin ha nam 2015 con chi 269,0 nghin ha nam 2018 (Nien giam Thong ke, 2018). Trong do, Hau Giang da tung duoc xem la mot trong nhimg vimg dat co dien tich mia duong lon nhat ciia vung DBSCL. Tuy nhien, trong nhimg nam g4n day, dien tich mia cung giam dan do nhi^u nguyen nhan nhu gia ban va anh hirong cua xam nhap man. Theo thdng ke ngi bp nam 2020 dien tich canh tac mia b Hau Giang giam chi con khoang 6.000 ha, so vcri dien tich mia cua tinh nam 2010 la 13.173 ha. Hien nay, huyen Phung Hiep co dien tich h6ng mia lon nhat tinh Hau Giang chiem den 2.000 ha, dien tich dat canh tac mia nay chii yeu sii dung phan hoa hoc nen co the lam gia tang chi phi san xuat va anh huong bat loi den moi truong.
Canh tac mia duong can luong lon phan bon hoa hgc vi luong duong chat cay mia hap thu cao. Cu the la, luong hap thu N (kg N ha') 263-328 tai huyen Cii
' Bp mon Khoa hpc cay tr6ng. Khoa Nong nghiep. Trucmg Dai hoc CSn Tha
- Smh vien nganh Khoa hoc cay trong khoa 44. Khoa Nong nghiep. Truang Dai hpc Can Tho
-* Tmang Dai hpc An Giang. Dai hoc Quoc gia thanh pho Ho Chi Minh
Email: nqkhuong@cCu edu.vn; [email protected]
Lao Dung, tinh Soc Trang, 25L306 doi voi mfa to va goc tai Long My, tinh Hau Giang (Nguyfin Quoc Khuong va ctv., 2015a, b; Nguyen Quoc Khuong va Ngo Nggc Hung, 2014; 2015a). Tuy nhien, kha nang cung cap dam tir dat thap, voi 84-109 kg N ha"^
(Nguyen Quoc Khuong va Ngo Ngpc Hung, 2015b).
Ngoai ra, bon phan dam giiip tang sinh truang (Nguyen Qudc Khuong, Ngo Ngpc Hung, 2013;
Nguyen Quoc Khuong va ctv., 2014a) va nang suat mia tai DBSCL (NguyM Qudc Khuong va ctv., 2014b, c; 2016a, b; Nguyin Qudc Khuong va Ngo Nggc Hung. 2015c). Phan dam dugc sii dung voi lugng lon nen co the gay ra nhirng anh huong bat lpi cho moi tiirdng thong qua phat thai khi nha kinh (Degaspan etal., 2020; Lopes etal, 2018). Do do, de dap ling muc tieu canh tac mia duong ben vimg, bien phap sinh hpc can duoc nghien ciru de thay the mot phan ngudn phan dam hda hoc da sii dung nhu hien nay. Trong do. cac dong vi khuan noi sinh re mia dudng cd kha nang cd dinh dam va tong hop indole acetic acid (lAA) la mot trong nhimg bien phap tiem nang.
Hien nay, nhieu nghien cuu cho thay vi khuan ngi sinh rk tac dong tich cue d^n sinh truong cay mia, thdng qua kha nang tiet cac chat kich thich sinh trudng thirc vat nhu lAA (Da Silva et i gibberelbns. cytokmins (Chauhan et al, 20n^ 2015), dinh dam (Figueh-edo et al, 2017: Wei
•^^3/-. 2014), 110 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 7/2020
KHOA HOC C 6 N G NGHE
hoa tan lan k h o t a n ( d e S a n h F e r r a r a etal, 2012) v a mot so h o p c h a t k h a c n h u H C N . c h i t i n a s e . a m m o n i a , cellulose v a p e c t i n a s e ( R o d r i g u e s et al, 2016). DiSu nay gop p h a n t a n g t h a n h p h d n n a n g s u a t v a n a n g suat cay m i a ( C h a u h a n et al, 2 0 1 3 ) . T u y n h i e n . i i t4n d u n g k h a n a n g b a n d i a d e n t i n h t h i c h n g h i c u a cac dong vi k h u a n . v i e c a p d u n g c a c c h u n g vi k h u a n diich n g h i v o l m o i t n r o n g d a t c h u a a H a u G i a n g trong vifec g i a m t h i e u p h a n b o n h o a h o c t r o n g c a n h tac mia o D B S C L la c d n t h i e t . D o d o . v i e c p h a n l a p . tuyen c h o n c a c d o n g vi k h u a n t h i c h n g h i t r o n g di^u kien pH t h d p c u a d a t p h e n c a n h t a c m i a d u o n g la r a t quan trpng. Vi v a y . n g h i e n c u u d u o c t h u c h i e n n h d m xac dinh d u o c c a c d o n g v i k h u d n c o k h a n a n g c 6 dinh d a m v a t o n g h o p c h a t k i c h t h i c h s i n h t n r o n g thuc vat lAA o d i e u k i e n c h u a .
2. VAT IIEUVA PHUONG PHliP
2.i.vatu$u
Mdu rk cay mia duong duoc thu tai xa Kinh Cung. xa PhuOTig Binh va xa Hoa An ciia huyen Phung Hi^p. tinh Hau Giang.
Moi truong NFB (g L') g6m: 5 acid malic. 5 K^HPO,, 0.2 MgSO.,-7H20. 0,02 CaClj. 0.1 NaCl, 4.5 KOH. 4 ml FeEDTA (1.64%), 2 ml dung dich nguyen td vi lugng, 1 ml dung dich vitamin, 2 ml broraothymol blue 0,5% trong KOH 0,2 N va 20 agar.
Moi truong Burk's (g L') gdm: 10 Sucrose. 0.41 KH2PO,. 0,52 KzHPO,, 0,05 NajSO,,. 0.2 CaCl,. 0.1 MgSO,-7HjO. 0.005 FeSO,-m.fl, 0.0025 Na2MoO,-2H20 va 20 agar.
2.2. Phuong phap
Thu miu re. Mdu r i cay mia duong duoc thu tir muoi cay mia khac nhau trong mot raong mia duong vao giai doan 120 ngay sau khi trdng de tron lai thanh mot mdu. Cac mdu duoc ky hieu la PBl. FB2 va FB3 dugc thu tai xa Phuong Binh. cac mau KCl va KC2 tai Kinh Cung va mdu HAI tai Hoa An. huyen Phung Hiep. tinh Hau Giang. Mdu duoc tru hong dieu kien 4°C cho den khi su dung de phan lap vi khudn.
Phan lap vi khuan ndi sinh: Can 2 gram mdi mau rS da dugc rua sach bdng nuoc may. Mau dugc cat dianh doan nho khoang 1 cm, cho vao binh tam giac 250 ml, sau do them 10 ml cdn 96%. ldc nhe trong 10 phiit, mdu dugc rOa sach bdng nude cdt vo himg 3 lin (5 phiit/ldn), bd sung 5 ml calcium hypochlonde 2%, ldc nhe h-ong 10 phut. ROra sach mdu bdng nude
cat vd triing 4 lin (5 phut/ldn). Hut 200 pi a i a nude nia ldn thu 4 trai d^u tren cac dia chira moi trudng TYGA. u a nhiet do 30°C trong 48 gid. Cdc dia mdi trudng nay khong xuat hien khudn lac dugc xem la mdu da dat yen cdu khu trung. Gia nhuyfen cdc mdu bdng cdi chay vd triing. Them 1.0 ml nuoc cat vd trung vao cdi, khuay ddu va hiit 500 pi dich trich mdu cho vao cac dng nghiem chiia mdi tiudng NFB ban dac khdng N (moi nghiem thiic lap lai 3 lan). Day kin cac dng nghiem. u d 30°C khoang 2 - 4 ngay. Cac mdu cd mot Idp mang mdng gan be mat mdi trudng thi chiing td cd su hien dien cda vi khuan npi sinh.
Trai lop mdi hudng ban dac cd chiia vi khuan ndi sinh sang mdi trudng khdng dam tuong ling. u cr 30"C hong 48 gid. Cac khuan lac khac nhau xuat hien tren bd mat mdi b-udng dugc hep tuc cay chuydn 2-3 ldn sang cac dia mdi trudng tuong ung cho den khi cac khuan lac dat thuin.
Dac diem khuan lac: Md ta hinh thai khuan lac gdm man sdc. hinh dang, dang bia khuan lac va do ndi. Nhudm Gram de xac dinh gram vi khuan.
Phuong phap danh gia kha nang chiu mdi tivdng chua ciia vi khuan. Tdt ca 28 ddng vi khuan dugc dieu chinh ODJM - 0.5. hut 0.5 mL dung dich cua mdi ddng vi khudn da didu chinh OD66o= 0,5 cho vao dng nghiem chiia 9.0 mL mdi truang NFB. Sau 48 gid u. dung dich khudn dugc do tren may quang phd d budc sdng 660 nm.
Phuong phap dmh lupng dam. Hai muoi bdn ddng vi khuan cd kha nang sdng hong dieu kien chua dugc nudi trong mdi trudng Burk's khdng dam 0 dang Idng de danh gia kha nang cd dinh dam. Hut 0.5 mL dung dich cua mdi ddng vi khuan da dieu chinh ODsjo - 0.5 cho vao dng nghiem chua 5 mL mdi trudng Burk's Idng khdng dam. sau dd ldc vdi tdc dd 120 vdng/phut g didu kien tdt. mdi ddng vi khudn cd 3 ldn lap lai. Dung dich mdi trudng Burk's khdng cd vi khuan duoc sir dung la mdu ddi chiing. Sau 48 gid u. hut 1.0 mL dung dich vi khuan de ly tam 15 phut d tdc dd 10.000 vdng phut'. Dung dich sau ly tam dugc dinh lugng dam bdng phuong phap hien mau blue phenol (Nelson etal., 1983), sau dd do mdu hen may quang phd g budc sdng 640 nm.
Phuong phap dinh lugng / I L Cac ddng vi khuan duoc tuydn chon cd kha nang chiu dmrc mdi trudng chua va kha nang cd dinh dam dugc su dung de danh gid kha nang cung cap lA-\ trong mdi mrdng NFB. pH 4.0. Bo sung 100 pg/L hen chat Uyptophan
N6NG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 7/2020 I I I
KHOA HOC C d N G NGHE
nhdm hd frg tdng hgp lAA. Cho 1.0 mL dung dich cac ddng vi khuan cd gia hi ODea, d' 0,5 vao dng nghidm cd chua sSn 9.0 mL mdi trudng NFB da cd tryptophan va u trong 48 gid. mdi ddng vi khudn dugc thuc hien voi 3 lap lai. Dung dich mdi trudng cd tryptophan ma khdng cd bo sung vi khuan dugc su dung ldm mdu ddi chiing. Sau dd hut 1.0 mL dung dich khuan de ly tam d tdc do 10.000 vong phur' trong 15 phut va ham lugng lAA dugc xac dinh bdng phugng phap so mau Salkowski va duoc tdm tdt nhu sau: 0,75 mL dung dich trich da dugc ly tam trdn vdi 3.0 mL tec chat Salkowski (4,5 g/L FeC13 hong 10,8 M H2SO4) duoc tl trong 20 phut a nhiet dp phdng. Ke ti^p, ham lugng lAA dugc xac dinh g budc sdng 535 nm (Glickman and Dessaux. 1995).
Xti ly sd heu: Sti dung phin mem SPSS phien
ban 16.0 so sanh khdc biet trung binh va phan tich phuong sal bdng kiem dinh Duncan.
3 . KET QUA Vit THAO LUMI
3.1. Phan lap va d$c dilm cua vi khuin ndi sinh
rk cAy mia dudngKdt qua phan lap dugc 28 ddng vi khuan ndi sinh rd cay mia dudng. Trong dd. miu rd PBl phan lap dugc cac ddng vi khudn PBld. PBle, PBlf. mdu PB2 gdm PB2b. PB2c2. PB2d. PB2e. PB2f va mdu PBS gdm PB3b, PB3d. PB3e, PB3f. Tuong hi tir mdu KCl phan lap dugc cdc ddng vi khuan KClal, KClbl.
KClb2, KClc. KCld, KCle. KClf va miu KC2 phan lap duoc KC2c, KC2d. KC2e. KC2f va miu HAI phan lap dugc HAla, HAlb. HAlc. HAle, HAlf.
Dac didm cda cac ddng vi khuan ndi sinh phan (a)
lap tir rd cay mia dudng dugc xac dinh b bdng 1. Cac khuan lac cd man hdng hong, trdng due hoac vang nhat sau 24 gid nudi ciy hen mdi hudng NFB. Trong do, khudn lac mau hdng due chiem so lugng Idn nhdt. voi 24/28 ddng. chidm 85,7% frong khi dd khuan lac mau hing hong va vang nhat chiem ti le thap hon, vdi 10.7% (3 ddng) va 3.6% (1 ddng), theo thii hr. Cic khuan lac dugc phan lap tren mdi trudng NFB ddu cd bia nguyen. vdi dudng kinh khuan lac chii yeu 0.5-1.5 mm. Tat ca cac ddng vi khuan cd dang lai.
Bang 1. Hinh thdi khufc l»c aia cdc ddng vi khuan phan Idp tir moi tnidng NFB
10 0-
7 5 -
1
2 5 -
/ / A
1
/ ^
MeBi\ = Std Dev
\
N = 0.66
=0 338 28
^
Dac didm
Mau sdc
Dudng kinh khuan lac
(mm) Dang bia
Ddndi
Hmh thai khudn lac Trdng due Trdng
trong Vang nhat
0.5-1,5
>1,5 Nguyen Rang cua
Lai Md
Sd lugng (ddng) 24
3 1 24 4 28 0 28 0
Tile
(%)
85,7 10,7 3.6 L 85.7
14.3 100 0 100
0
3.2. Tuy& chpn vi khudn ndi sinh rS ciy mia dudng cd kha nang chiu dugc mdi trudng chua vd chiic nang cd djnh dam
0 5 10 15 I'o Ham hivng dun ( m ^ )
Hinh 1. Tdn sait dia cdc ddng vi khuin cd kha nang (a) chju dupc difa kifn chua vd (b) cd dinh dim
tr*n mdi tmdng NFB
112
NONG NGHIEP VA PHAT TRIEN NONG THON - KV 2 - THANG 7/2020KHOA HOC CdNG NGHC
Danh gid kha nang chiu mdi hirdng chua cda 28 dong vi khuan, ket qua cho thay tat ca cac ddng ddu c6giatriODBa,nhdhgnl,5 (Hinh la).
Trong dd. cd 4 ddng vi khudn thich nghi tdt nhit trong didu kien chua Id PBld, PB2d, PB4d va KC2c. Bdn canh dd, cd 4 ddng vi khudn cd gia hi ODMO be hon 0,2. nghia la bdn ddng vi khudn nay cd thich nghi rdt kem h o n g dieu kien chua. Vi vay. chi CO 24 ddng vi khudn dugc su dung de danh gia kha nang cd djnh dam. Ngoai ra. dieu dang chii y la tin suat cua cdc ddng vi khuan chiem da sd o khoang ODjao 0.75-1,0.
Trong 24 ddng vi khuan chiu pH chua vdi kha nang cd dinh dam da dang, hdu het cac ddng nay cd kha nang cd dmh dam thap vdi ham lugng dam nhd hon 5,0 mg/L (Hinh lb). Tuy nhidn, trdn mdi tnidng NFB cdc ddng vi khuan cd su bidn ddng rat Idn vd kha nang cd dinh dam. vdi ham lugng 0,47- 18,5 mg/L va trung bmh 5,36 mg/L. Ben canh dd.
nam ddng vi khuan cd khd nang cd dinh dam lon hon 10.0 mg/L (Hinh l b ) .
3.3. Ddnh gid khd nang cd dinh dam vd tuydn chpn vi khudn n$i sinh r^ cdy mia dudng cd kha nang tdng hgp lAA dupc phdn Idp tir mdi trudng NFB
|.
j
b
il
efQd de 1
iiiJi
• ) * ' ^ ^ ^ ? * c'^' < * ^ ^ Ten cac diing \i khuan
Hinh 2. K h a n S n g c 6 d i n h d a m c u a cAc d o n g vi k h u & i nOi s i n h rk mia dubng T r o n g m o i t r u o n g N F B l o n g , 13 t r o n g sd 2 4 c a c dong vi k h u a n n p i s i n h c o h a m l u o n g d a m c o d i n h t o t nhat diroc t r i n h b a y 6 h i n h 2. T r o n g d o , d o n g vi khuan K C l c d a t k h a n a n g c o d i n h d a m c a o n h a t (18,5 m g / L ) , b d n d o n g vi k h u a n c 6 k h a n a n g c d d i n h a^m t h a p h o n la P B 3 b , K C l f , K C 2 d , K C l b l vdi h a m lugng 12,1-14,2 m g / L C a c d o n g vi k h u a n c o n lai c o kha n a n g c d d i n h d a m t h a p , voi 3,07-8,98 m g / L (Hinh 2 ) . K ^ t q u a n a y c h o t h a y d a y la c a c d d n g vi
k h u a n c o k h a n a n g c u n g c a p d a m t r o n g d i e u k i ^ n m 6 i t r u a n g p H t h a p .
D o n g vi k h u a n n6i s i n h H A l e c o k h a n a n g t d n g h g p lAA c a o n h a t , k h a c bi^t y n g h i a t h d n g kt 5% s o voi c a c d o n g vi k h u a n c o n lai, v m h a m l u g n g LAA t u o n g l i n g la 29,38 v a 17,84 - 4,00 m g / L . T r o n g d o . d o n g vi k h u a n P B 2 e co h a m l u o n g lAA c a o ti^p t h e o , voi 17,84 m g / L t r 6 n m o i t r u a n g N F B ( H i n h 3 ) .
HAII HAle PB2e KCld Ten cac dong n khuan H i n h 3. K h a n d n g t 6 n g hgp lAA ciia vi k h u ^ nOi
s i n h rk m i a d u 6 n g
H i e n n a y n h i e u d o n g vi k h u a n n d i sinh cSy m i a d a d u g c p h a n lap v a t u y ^ n c h g n d^ h 6 trgr s i n h t n r o n g cay mia. C u t h e la, d o n g vi k h u a n Klebsiella variicola DX120E d u g c p h a n l a p t u rk g i d n g m i a R O C 2 2 t r d n g tai T r u n g Q u d c c o k h a n a n g co d m h d a m ( U n et al., 2012; W e i et al, 2 0 1 4 ) . K ^ t q u a t m m g t u d i n g dugrc t r i n h b a y b o i T a u l e et al. (2019), d o n g vi k h u 3 n Kosakonia s p . UYSOIO d u g c s u d u n g Ak t h i i c d a y s u s i n h t r u o n g c u a c a y mia. D d i voi k h a n a n g t o n g h a p lAA, c a c d o n g vi k h u a n Klebsiella sp. K F A 1.3, K F A 1.2, KRC 2.2. KRB 1.2 d u g c p h a n l a p tir t h a n , la v a rk m i a d u o n g v a o t h a i d i e m 6 t h a n g tuoi a Brazil c o tXkra n a n g c u n g c a p lAA c h o c a y t r d n g , voi n d n g d o 109-128 p g m l ' ( R o d r i g u e s et al., 2 0 1 6 ) . N g o a i r a , d o n g vi k h u a n ngi s i n h c a y m i a Pantoea agglomerans 33.1 tiet r a lAA 100 p g / m l ( Q u e c i n e etal., 2 0 1 2 ) . M o t sd n g h i e n c m i c u n g c h o t h a y v i e c t u y e n c h o n vi k h u a n d u g c t h u c h i e n d e c h g n c a c d o n g vi k h u a n c o n h i e u chiic n a n g k h a c n h a u . C u t h e la, t h e o n g h i e n c u u c u a M o d i v a P a t e l (2017), d o n g vi k h u a n F 1 8 1 , F 2 7 1 , F 3 2 3 , F372, F 3 7 3 , F 5 3 1 , E L B l v a E S B 4 c o c a c c h u c n a n g n h u h o a t a n l a n , t d n g h g p LAA, s i d e r o p h o r e s v a c d d i n h d a m , vi v a y c a c d o n g vi k h u a n n a y giiip t a n g n a n g s u a t m i a . Viec b d s u n g d o n g vi k h u a n Bacillus spp. H 1 5 giiip t a n g 42% n a n g s u a t m i a ( C h a u h a n et al., 2013) c u n g n h u d o n g vi
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 • THANG 7/2020 113
KHOA HOC CONG N G H |
khuan Delftia acidovorans IAC-BECa-023 tang hdm lugng dam (da Silvemi et al, 2019). Ben canh dd.
viec dp dung ddng vi khuan Pseudomonas sp.
SugS_49 giup tang nang suat lua mi hong didu kien nha ludi (Pirhadi et al., 2018). Ngoai ra, su dung ddng vi khuan ndi smh cay mia Klebsiella sp. KFA 1.3 giup tang sinh khdi khd cua than va rd bdp (Quecine etal, 2012). Dieu ndy cho thay hem nSng ciia cac ddng vi khuan da dirge phan lap va tuyen chon trong nghien cuu ndy cd the ap dung tren cac loai cay trdng khac. Qua cac nghien cuu tren cho thdy. cac ddng vi khuan ddu giup cay trong phat trien hon. Nghia Id. cac ddng vi khudn cd thd cung cap bd sung dmh dudng va cac chat kich thich sinh truong thuc vat cho cay trdng. Trong nghien cuu nay. cac ddng VI khuan dugc tuyen chpn cho mdt chuc nang manh nhat dong thdi cung cd cdc chiic nang cdn lai nhung g muc dp thap hon.
4. KET LUAN VA BE NGHI 4.LK^tluan
Tren mdi trudng NFB da phan lap dugc 28 ddng vi khudn ndi sinh rd cay mia dudng trdng tren ddt phen tai huyen Phung Hiep. tinh Hau Giang. Tuyen chpn dugc 5 ddng vi khudn cd kha nang cd dinh dam bao gdm KClc. PB3b. KClf. KC2d. KClbl vd 3 ddng VI khuan tiem nang tdng hgp lAA nhu HAle. PB2e va KCld trong dieu kien chua. Cdc ddng vi khudn ndi sinh rd cay mia dudng cung cap dugc lugng dam va lAA Idn lugt la 12.1 - 18,5 m g / L va 12,1 - 29,4 mg/L.
Day Id cdc ddng vi khudn hem nang su dung trong canh tac mia g DBSCL.
4.2. Dd nghi
Dinh danh cac ddng vi khuan tidm nang da dugc tuyen chpn de ung dung vdo hong canh tdc mia duong trdng h e n dat phen.
TAI UEU n i A M KHAO
1. Chauhan. H.. Bagyaraj. D. J.. Sharma. A (2013). Plant growth-promoting bacterial endophytes from sugarcane and their potential in promoting growth of the host under field condiUons. Expenmental Agriculhire. 49(1): 43-52.
2. Da Silva. J. F , Barbosa. R R.. de Souza. A.
N., da Motta. 0 . V.. Tebteira. G. N.. Carvalho, V. S..
de'Souza. A. L S. R.. de Souza Filho, G. A. (2015).
Isolation of Pantoea ananatis from sugarcane and characterization of its potential for plant growth
promohon. Genetics and Molecular Research. 14 (4):
15301-15311.
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ISOLATION AND SELECTION OF NITROGEN FIXING, INDOLE ACETIC ACID PRODUCING BACTERIA FROM SUGARCANE ROOT IN ACID SULFATE SOIL
Nguyen Quoc Khuong, Le Vmh Thuc, Le Thi My Thu, Luu Thi Yen Nhi, Vo Van Ung, Tran Chi Nhan, Ly Ngoc Thanh Xuan, Nguyen Thi Thanh Xuan
Summary
Use of bacteria is one of the most potential meUiods for sustainable cultivation of sugarcane by reducing the volume of chemical fertilizers. The objective of tiiis research was to select the endophytic bacteria from sugarcane root possessing the ability of nitrogen fixation and lAA production. Six sugarcane root samples grown in acid sulfate soil were collected for isolating bacteria in Phung Hiep district. Hau Giang province. The results showed that 28 strains of endophytic bacteria were isolated from medium NFB.
with 24 stiains having the ability of resistance to acidity. The selected bactena of nitrogen (bdng function were included stiains KClc. PB3b. KClf. KC2d and KClbl. witti their concentiations 12 1-18.5 mg/L. and chosen strams of lAA synthesis were included HAle. PB2e and KCld. with their contents ranging 12.1 - 29.4 mg/L. It is recommended that mixed cultures should be used as plant growth promoters for synergic roles to support sugarcane growth and yield, although they possess both functions.
Keywords: Acid sulfate soil endophytic bacteria, niuogen-hxing, lAA, sugarcane.
Ngudi phan bidn: PGS.TS. Ld Nhu Kidu Ngay nhjnba: 13/3/2020 Ngiy thdng qua phan bidn: 14/4/2020 Ng^y duydt dSng; 21/4/2020
1,6 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 7/2020
