TAP CHl KHQA HQC VA CQNG NGH£. Tnr&ng Dai hpc Khoa hpc-DH Hue Tip 7. So 1 (2017)
N G H I E N C U t I H O A T T I N H K H A N G O X Y H O A V A C A U T R U C C U A P O L Y S A C C H A R I D E T A C H C H I E T T U * N A M T H l T O N G H O A N G
{PheUinus linteus ( B e r k . E t C u r t . ) T e n g )
T r i n Thi Van Thi*, Lg L i m Son, Tran V i n Khoa, H I Minh Tung Khoa Hda hpc. Truang Dgi hpc Khoa hgc - Dgi hpc Hui Emad: tranthivanlhi@gmailcom T O M TAT
Hoat tinh khdng oxy hoa cua polysaccharide (PS) tdch chiet lir nam Thuang hodng (Phellinus linteus (Berk, et Curt.) Teng) dd dupc nghien cuu. PS sau khi chiit xudt vd linh che duac hda tan lgi vdo nude vd dupc kit tua ldn lugl trong ethanol 32", 48° vd 68°. thu dugc cdc phdn dogn tuang ung la PS-E32. PS-E48. PS-E68. Hogt tinh khdng oxy hoa cua cao PS. PS-E32, PS-E48, PS-E68, cao ethanol vd cao nude dupc ddnh gid theo khd nang cho electron vd khd ndng bdt gdc tir do I, I-diphenyl-2-picrylhydrazyl (DPPH). Kit qua cho thdy, so vai curcumin, hogt tinh khdng oxy hoa theo ca chi cho electron cua PS chiit xudt tir ndm Thupng hodng thdp han, nhimg hogt tinh thi hiin qua bat gdc Hr do DPPH thi cao ban. Kit qud khdo sdt cdu true polysaccharide Irong phdn dogn ethanol 48°xdc nhpn PS- E48dupc tgo thdnh tir D-mannose, D-glucose. D-xylose. D-galactose, D-arabinose vdi ty le mol tuang img Id 1,00: 0.29: 0,12: 0.08: 0.06. PS-E48 co bp khung chinh Id mannan, chira chd yiu la lien kit l-*6-D-mannoside. mgch nhdnh co chira cdc lien kit 1^6-D-glucoside vd l-*6-D-galactoside.
Tir kh6a:cdu truepolysaccharide.hogt tinh khdng oxy h6a,Phellinus lintues.
L M d D A U
Nim Thugng hoing (Phellinus linteiis(Berk. et Curt.) Teng) li loai nam g l ty nhien, hifn dang nhgn dugc sy quan tam die bift do cd nhieu hoat tinh sinh hgcquy, hua hen vugt frgi hon ca cac loai Linh chi: khang khdi u, uc chi tl bio ung thu, ting cudng khang thf, khang oxyhda [l-10].Ngay nay, ngudi ta daphan lap giong, thuin dudng v i nudi frdng thanh cdng.
Hifn nay, nam dugc frdng tgi Han Quic, Nhgt Ban, Trung Quoe va budc dau da dugc frdng d Viet Nam.Ciing mgt loai nhung dugc nudi trdng trong difu kien khic nhau,thanh phin va ham lugng cac boat chat frong nam cd thf thay ddi. Do dd, cic miu nam Thugng hoang cd nguon glc khac nhau cd tilled hoat tinh sinh hpc khac nhau. Polysaccharide (PS) la thanh phin hogt chitquan frgng frong nam Thugng hoing. PS la cac polymer thifn nhifn cd can tnicda dgng, phuc tap va d i dugc nghien cuu rpng rii frong y hpc do cic hogt tinh sinh hpc da dang ciia
Nghiin cuv hogt tinh khang oxy hoa vi cau tnie cua polysaccharide tach chiit tir nim Thupng hoing...
chiing [1 IJ.Trong bai bao niy, chung tdi thdng bao nhihig kit qua khio sat diu tifn vf hogt tinh khang oxy hda ciia cao ethanol, cao nude, cao polysaccharide, ddng thdi xac dinh can tnie ciia polysaccharide frong cao phan doan ethanol 48°tii mau nam Thugng hoing (Phellinus linteusiBerk. et Curt.) Teng) frong tai mifn NamVift Nam.
2. NGUYEN LIEU VA PHlTONG PHAP NGHIEN CtTU 2.1. Nguyen lieu
- Nim Thugng hoang duac mua td frang trgi sin xuat tai thanh phd Hd Chi Minhd dang qua thi da say khd va ddng gdi theo tifu chuin cua nha sin xuat. Ten Ioai da dugc nha cung cap giong va nhi san xuit xic nhan la Phellinus Ilnteus{Berk. et Curt.) Teng.
- Nim Dui ga (Pleurotus eryngii): gdm miu cd nguin gdc td Trung Quic vi miu dugc frdng tgi Da Lgt.
2.2. Chiet xuat cao ethaool, cao nirdc, cao PS, tinh chf PS va phSa doan
- Chilt xuit cao ethanol va cao nude; Mau cic loai nam dugcxay nhd,ngam chiet vdi ethanol (EtOH) 96° frong hai tuin dk tach logi cac hgp chit tan frong ethanol, cd duli dung moi, thu dugc cao ethanol. Sau dd, miu tiip tyc dugc chilt bing nude d 1OCC trong 4 gid. Qui trinh chilt dugc lap di lap Igi cho din khi dich chilt cudikhdng cdn polysaccharide (djnh tinh bing phenol-acid sulfiiric [I2]).Cd logidung mdi, thu dugc cao nude.
- Tich chilt va tinh chi cao polysaccharide (PS): Hda tan vdi nude cat viia dii, ket tiia vdi ethanol96° theo ^ If 1:5 (v/v), df qua dfm d-4''C rdi tien hanh ly tam thu lay PS tho. PS tho dupc tinh ehf bing phuong phip chilt vdi hdn hgp thudc thu Sevage (CHCIgiBuOH = 4:1, v/v) trong 30 phiit dk logi protein ty do.Qui trinh tinh chi logi protein niy dugc lip Igi nim lan den khi khdi lugng cao thu dugc gin nhu khdng dii. Sau khi Iy tam, phin tua lin lugt dugc nia sach vdi ethanol tuyft ddi vi acetone trude khi siy khd, thu dugc cao PSdatinh che.
- Phin dogn PS; Hda tan polysaccharide (PS) da tinh che vao nude cat vua dii, ket tiia lai bing ethanol 96" vdi cicty If thi tich djch nudc:ethanol la 1:0,5, 1:1, 1:2,5 tuang iing vdi ndng dd ethanol khic nhau 32°, 48°, 68° dk cd cic phan dogn polysaccharide khac nhau, ky hifu lin lugt la PS-E32, PS-E48, PS-E68.
2.3. Phutfng phap danh gia ho^t tinh khang oxy hoa
2.3.1. Xac dinh khi ning cho elefron theo lyc khang oxy hoi ting (total antioxydant capaci^) frong md hinh phosphomolybdenum
Lyc khing oxy hoa tdng ciia cac cao ethanol, cao nude, cao PS va cic phan dogn PS-E32, PS-
£48, PS-E68 tach chiet td nam Thugng hoing dugc danh gia theo phuang phap phosphomolybdenum ciia Prieto [2]. Phuong phap niy dya tren sy khu Mo (VI) vf Mo (V) bdi cac hop chit cd kha ning khing oxy hda frong mdi frudng acid, tgo thanh phuc phosphate/Mo
TAP CHlKHQA HQC VA CONG NGH£. Tn/mg Dgi hpc Khoa hpc-DH Hui Tip 7, Si 1 (2017) (V) cd mau xanh la ciy. Liy 0,3 mL dich mau khao sat, thfm vao 3 mL dung dich thudc thu (0,6 M acid sulfiiric, 28 mM sodium phosphate va 4 mM ammoni molybdate), day kin va u d 95°C frong 90 phiit. Sau do, mau dugc lam Ignh ve nhift do phdng. Df hip thu ciia dung dich sau phan ung dugc do d budc sdng 695 nm frfn may quet phd tii ngogi - kha kiln UV-Vis DR Jacos V630.Trong miu t i ^ g , dung dich cin phin tich dugc thay bing nude cit. Lyc khang oxy hoa tdng dugc bifu difn theo do hap thu ciia man, dg hip thy cang ldn thi lyc khang oxy hda tdng cang cao.
2.3.2. Danh gii lyc khang oxy hda qua kha ning bit glc ty do DPPH
Dya vao kha nang cic chit kliang hda chuyln hda glc ty do l,l-diphenyl-2- picrylhydrazyl (DPPH) thanh sin phim phin td lam cho dung dich chuyln tu mau tim sang vang. Pha dung dich DPPH ndng do 100 pM frong methanol ngay trude khi diing. Hdn hgp phin ling cd thi tich 3000 pL, gdm 1500 fiL miu khao sit d cac ning dp 100 fig/mL; 20 pg/mL; 4 pg/mL; 0,8 fig/mL va 1500 pL dung dich DPPH ndng dp 100 fiM. Cac hdn hgp phin ling dugc lie frong 1 phut va u d nhift do phdng frong 3D phut, rdi tifn hinh do quang d budc sdng 517 nm [9], Cdng thuc tinh:
SADPPH (%) = [(Ac -As)/Ac] x 100 Trong do:
SA DPPH (^): tic dyng bit gdc ty do (Scavenging Activity) DPPH cua miu.
As : mit do quang cua mau khio sit Ac: mgt dp quang ciia mau frang
Tit ca thi nghiem dugc lap Igi 3 lin. Tac dung bit gdc tu do dugc danh gia qua gia frj ICSO.MIU nim Diii ga (Pleurotus eryngii) glm miu cd ngudn gdc tii Trung Qudc vi man dugc frdng tai Da Lat ciing dupc khao sit df so sinh.
2.3.3. Xac dinh ciu tnie polysaccharide
- Methyl hda: Can 30 mg miu hda tan frong 2 mL dunethyl sulfoxide (DMSO) cho vao binh ciu hai cd cd lip hf thing hdi luu, khuiy tii frong 3 gid. Sau do them 30 mg NaOH tiep tuc khuiy tiJ frong 10 phut, lam lanh d 5''C, them tiip 17 mL (CH3)2S04 vio v i tiip tuc khuiy frong 16 gid. Chilt PS da metiiyl hda bing hon hgp CHCI3: HiO= 2:1 (v/v).
- Thuy phan: Liy 5 mg miu PS da methyl hda thuy phan vdi 3-4 mL dung dich acid CF3COOH 4M tgi 120°C trong 2 gid. Sau do, hdn hgp dugc lam mat vl nhift dg phong, rua bing CHjOH vi lim khd bing khi N2.
- Khu hda: Thfm 4 mL NaBHi 0,25M pha trong dung dich NHiOHIMvao mau sau khi thuy phan, giir d nhift dg phdng frong 30 phiit. Sau do thfm 5 mL dung djchacidCHsCOOH 10% trong CH3OH vio dl trung hoa lugng NaBHi du. Hdn hgp dugc thoi khd bing ddng khi N2.
Nghiin cuv hoat tinh khing oxy hoa vi ciu tnie cua polysaccharide tich chiit tir nim Thirpng hoing...
- Acetyl hda: s i n p h i m da bi k h u h d a d u g c acetyl h d a v o l 1 m L hdn h g p (CH3CO)20;
C5H5N (pyridme) = 1:2 (v/v) tgi 100°C trong 2 gid. D f ngudi h d n hgp, them 5 m L CHsOHvalim khd bang ddng N2. Chiet san p h i m da acetyl h d a b a n g ethyl acetate [13].
M a u d u g c p h a n tich fren thiet bi s i c ky khi ghep khdi phd G C - M S Agilent 7890A sir d u n g cdt tach HP-5MS (30 m x 0,25 mm x 0 , 2 5 ^ m ) , khi mang H e v d i tdc do ddng 1 mL/phut.
C h u o n g frinh nhift d p : tii 120 "C nang nhift d o Ifn d i n 150 °C vdi tdc dp 10 "C/phut, tiip tgc nang nhift dp Ifn dfn 220 "C vdi tdc dp 3 "C/phut, sau d o nang nhiet d o d i n 280 "C vdi t i c do 10 "C/phiit. Detector M S su dung n g u i n ion hda EI, nhift do n g u i n d 230 ""C, vdi c h i dp phan tich scan.
3 . K E T Q U A V A T H A O L U ^
3 . 1 . K h a Dang k h a n g oxy b o a c u a p o l y s a c c h a r i d e t a c h chiet tir n i m T h u g n g h o a a g 3.1.1. Kha nang cho eleefron theo lyc k h i n g oxy hda tdng frong md hmh phosphomolybdenum
Ket qua x a c dinh lyc khang oxy h o i tong cua c a o PS d u g c trinh bay frong hinh 1.
D<iliSpthy(A) • N a m Thinjng hoang
n NSm Dili s^ Viet Nam N l m Dili ga Trung Quoe
• CurcuminB
l » I . l l
0-^N6ngdoO»g/mL9'5
Hinb 1. Lyc khang oxy hda tdng ciia cao PS cic mau namvi curcumin
T u h m h 1, cho t h i y lyc khang oxy hda tong cua cao P S c h i l t t u c i c m i u n i m dfu tang theo chifu tang nong do k h i o sat.
Trong p h u a n g phap xac dinh kha nang cho electron thdng qua lyc k h i n g oxy hda ting theo md hinh n i y , so vdi curcumin-mpt Ioai d u g c lifu cd boat tinh khang oxy hda tdt thi PS nam T h u g n g hoang cd lyc khang oxy h d a t i n g thap h a n . Tuy nhifn so vdi m i u n i m t h y c p h i m nhir n i m Dui g i (Pleurotus eryngii) t u Trung Q u i c va n i m Diii ga {Pleurotus eryngii) frdng tgi Viet
TAP CHl KHOA HQC VA CONG NGHB. TmOng Dgi hgc Khoa hpc - DH Hui Tip 7. Si 1 (2017) Namthi PS nam Thugng hoang cd lyc khang oxy hda tdng cao hon rat nhilu, dac bift li d ndng do cao.
3.1.2..Khi nang bit gdc ty do DPPH
Kha nang bat gdc ty do DPPH cua cao PS nam Thugng hoang (Phellinus linteus) dugc danh gia qua ty If bit gdc tu do DPPH va gia tri ICso, kit qui trinh bay tai hinh 2.
% I N S m Thugng hoang s Nim Diii g i Vi£i Nam 100 • Nlm Qi„ ga Tning Quae
Curcumiiie
11 II I I . I
Hinh 2. Ty If phin fram bit glc ty do vi gii tri ICjoCua cao PS cic mau nim vi curcumin s l lifu tii hinh 2 cho thiy, t^ If bit glc ty do DPPH ciia cao PS tii nim Thugng hoang (Phellinus linteus) tang khi tang nong dp, tuy nhifn d nong dp thip thi sy bien ddi li khdng dang kf. Gia tri ICsociia cao PS td nim Thugng hoang (Phellinus linteus) khdng nhdngthap hon so vdi cic cao PS td nam Diii gi (Pleurotus eryngii) td Trung Qudc va Viet Nam ma cdn thip hon han so vdi curcumin. Day cung la dau hifu cho thay tiem nang iing dung Iam dugc lifu ciia nam Thugng hoang (PheUinus linteus).
Cho dfn nay, chiing tdi chi tim dugc cdng bd [1] su dung phuang phap DPPH de xac dinh khi nang bit glc ty do ciia polysaccharide frong nam Tliugng hoang vdi ECjo la 7,11 mg/mL so vdi chit chuin acid ascorbic cho tin hifu hogt dgng tdt tgi EC;o cd gia tri nong dd 1,64 mg/ mL. Nhu vay, ket qua khao sit ciia chung tdi theo md hinh phosphomolybdenum vi ket qua khao sat theo md hinh DPPH cua cdng trinh [1] deu cho ket qua dang khich If ve kha nang hogt dgng khang oxy hda cua polysaccharide tii nam Thugng hokng(Phellinus linteus).
3.1.3. Kha nang khang oxy hda cua cic cao PS phan dogn
Ket qui xac dinh luc khang oxy hoa tong cua cac cao phan doan polysaccharide dugc thi hifn trfn hmh 3.
Nghien ciiv hogt tinh khing oxy hoa vi ciu tnie cua polysaccharide tach chiit tir nim Thutyng hoing ..
DQhaplhv(A) 0.45
• PL-W BPL-E •PS-E32 • PS-E48 • PS^68
lllil lllll ll III
Hinli 5.L1JC khing oxy h6a tfing ciia cic phin dogn cao PS, cao Pl-E vi cao Pl-W tai cic n6ng df khic nhau, Td kit qua d hinh 3cho thiy ring, hoat tinh khang oxy hda cua cac phan dogn deu tang theo ning dd. Hoat ti'nh khing OTty hda ciia cao ethanol (Pl-E) va cao nude (Pl-W)ludn ludn cao hon cic cao phan dogn PS, frong do lyc khing oxy hda mgnh nhat la cao ethanolva sau do la cao nude. Td kft qua niy chung tdi cho ring, cao ethanol chda nhieu cac hgp chat cd kha nang khang oxy mgnh nhu cac hgp chit phenolic, flavonoid..., cao nude khdng nhiing chiia polysaccharide mi cdn chda mdt lugng cac chit khang oxy hda mgnh khic, chiing da ddng gdp mdt phin frong hogt tinh khing OJQ' hda nay.
3.2. c i u triic cua polysaccharide trong phan dogn PS-E48
Dya vio sic ky dl GC-MS, chiing tdi xac dinh dugc cac dan xuat monosaccharide.
Thdng sl ciia cic din xuit monosaccharide dugc chi ra trong bang 1 va 2.
Bang 1. Cdc din xuit monoDsaccharide cua PS'E48 thi higu trSn sic do GC-MS Hap chat
l,3,4,6-tetra-O-acetyl-2,5-di-0-methyl- D-maimitoI
l,5,6-tri-O-acetyl-2,3,4-tri-0-methyl- D-glucitol
5,6-di-0-acetyl-2,3,4-tri-0-methyl- D-mannonitrile
2,3,4-tri-O-methyl-D-xylopyranose l,5,6-tri-O-acetyl-2,3,4-tri-0-methyl-D- galactitol
2,3,4,6-Ktra-O-methyl- D-mannopyranose
ThM gjan Ivu
(phut) 11,092 25,173 23,652 22,551 24,237 21,644
Dien tich
peak Lo^i li£n kit cua monosaccharide trons PS 2936700 l->3,6-
D-mannofuranoside 1202748 1^6-
D-glucopyranoside 756432 l-»6-
D-mannopyranoside 496907 1-D-xvlopyranoside 436343 l->6-
D-galactopyranoside 353855 -> 1-D-maimopyranoside
TAP CHl IQiQA HQC VA CQNG NGH$. Tw^g Dgi hoc Khoa hpc - PH HuJ Tgp 7. Si 1 (2017)
2,3,4,5-tefra-O-acetyl- 27,600 282635 1 ^ 2 , 3 , 4 -
D-arabinononitrile D-arabinopyranoside Hogc 1^2,3,5-
D-arabinoftiranoside Tu kft qua phan ti'eh cic dan xuat cua monosaccharide bang GC-MC cho thiy thanh phin monosaccharide cd frong PS-E48 ciia nam Thugng hoang gom D-mannose, D-galactose, D-glucose, D-xylose vi D-arabinose. Lien kft 1~»6 glycoside chifm ty If cao nhat.
Td bang 1, chung tdi xic djnh dugc ty If thanh phin cac monosaccharide trong PS-E48, s l lifu dugc frinh biy d bing 2.
Bang 2. Ty le thdnh phan cac monosaccharide Thanh phan Ty le mol
D-maimose 1,00 D-glucose 0,29 D-xylose 0,12 D-galactose 0,08 D-aiabinose 0,06
cua PS-E48 TyIS(%)
62,59 18,60 7,68 6,74 4,37 Sd lifu td bang 1 va 2 cho thiy, thinh phin chinh eua polysaccharide frong PS-E48 ciia nim Thuang hoang Ja D-marmose vdi bd khung chinh cda PS-E48 la mannan, theo ty If D- mannose: D-glucose: D-xylose: D-galactose: D-arabinose = 1: 0,29: 0,12: 0,08: 0,06. Mgch chinh chua chu ylu la lien kit l-»6-D-maimoside, cac mach nhanh chua lien kit chu ylu la l-»6-D-gIucoside va 1^6-D-galactoside. Nghien cuu cua Juan-Juan Pei vi cdng sy [13] da tich dugc polysaccharide (PL-Nl) tii nim Thugng hoing d dgng sgi (hay dgng to chua hmh thanh qua till dugc trdng frong mdi trudng nhan tgo), PL-Nl sau khi phin tich cd thinh phan monosacharide la arabinose, xylose, glucose vi galactose vdi ty If: 4,0: 6,7: 1,3: 1,0. Bd khung PL-Nl ia lien kit (l-^4)-^-D-)0'Iopyranoside. Theo tac gia John R. Baker, liy djch chilt nude nim Thugng hoang phin doan bing (NH4)2S04, thim tach, qua eft DEAE-trisacryl (20 cm x20 cm) thi polysaccharide cd ciu true bd khung chinh la glucomannan, lien kit ^-(1^3)- mannopyranoside la lifn kit trong mach chinh va lifn kit frong mgch nhanh la lien ket (l->6).
4. KET LUAN
Trong nghifn cihi nay, kha ning khang oxy hda ciia cao polysaccharide tach chiet tu nim Thugng hoang (Phellinus linteus) hong tgi Vift Nam va caccao phin dognda dugc khao sat khi nang cho eleefron theo phuong phap phosphomolybdenum (Prieto, 1999) va khi ning bit glc ta do DPPH.Polysaccharide tich chilt tii nim Thugng hoing (Phellinus linteus) frdng tgi Vift Nam thf hifn kha nang cho electron cao ban nhilu so vdi cac loai nim thyc phim Dui ga (Pleurotus eryngii)tii Trung Quic va Vift Nam nhung thip hon so vdi curcumin. Dac bift kha nang cho hydro cua cao polysaccharide cua nim Thugng hoing (Phellinus linteus) cdn cao hon hin curcumm. Phin dogn PS-E48 cd bg khung D- mannan, vdi ty If mol D-mannose: D- glucose: D-xylose: D-galactose: D-arabinose == 1: 0,29: 0,12: 0,08: 0,06, cd mgch chinh chda
Nghien cOv hogt tinh khing oxy hoa vi cau tnie cda polysaccharide tach chiit tir nam Thuvng hoing ..
^ f y l u la lifn ket l->6-D-mannoside, cac mach n h i n h c h u a chu y l u la lien k i t l-*6-D.
g l u c o s i d e v a l-»6-D-ga!actoside.
L G f l C A M O N
Xin chdn thanh cdm an Phong thi nghiem Hoa hoc cdc Hap chdt thien nhien, khoa Hoa hpc, tru&ng Dgi hoc Khoa hoc Hue da ho tramoi dieu kign thugn lgi de co 3u^c nghien cim nay.
T A I LEEU T H A M K H A O
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[6].S. Sorasak, S. Prapairat, K. Chattai (2011). Two hidole Devatives and Phenolic Compound Isolate fix)m Mushroom Phellinus linteus. Chin. J. Nat. Med. Vol. 9, Iss. 3, pp. 0173-0175.
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[8]. Y. Yang, J. Zhang, Y. Liu, Q. Tang, Z. Zhao, W. Xia(2007). Structural elucidation of a 3-O-methyl- D-galactose contaming neufral polysaccharide from the fruitmg bodies of Phellinus igniarius.Carb.
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[9]. T. L. C. Oldoni, S. M. D. Alencar, A. Reis, A. D. Loguercio, R. H. M. Grande (2012). Antioxidant activity by DPPH assay of potential solutions to be applied on bleached teeth, Braz. Dent. J, Vol.
23, Iss. 1, pp. 22-27.
TAP CHl KHQA HQC VA CONG NGH£, Tnrimg Dgi hpc Khoa hgc - DH Hui Tgp 7, Si 1 (2017)
[10].T. Zhu, K. S. H. Kim, C. Y. Chen (2008). A Medicinal Mushroom: Phellinus Lintues, Current Med C/iem., Vol.15, pp. 1330-1335.
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S T U D Y O N ANTIOXTOANT A C T I V I T Y A N D S T R U C T U R E OF P O L Y S A C C H A R I D E E X T R A C T E D F R O M T H U O N G H O A N G M U S H R O O M
(Phellinus linteus (Berk. Et Curt.) Teng)
Tran Thi Van Thi*, Le Lam Son, Tran Van Khoa, Ho Minh Tung
Department of Chemistry, Hue University College of Sciences Emad: [email protected] ABSTRACT
Antioxidant activities of polysaccharide extracted from Thuong hoang mushroom (Phellinus linteus (Berk. Et Curt.) Teng) were studied The polysaccharide (PS) was extracted and purified to remove free protein. The PS was resolved in water and precipitated fy 32°, 48' vd 68 ethanol solutions, yield PS-E32. PS-E48, PS-E-68 fractions respectrvely.The antioxidant capacity of PS, PS-E32. PS-E4S, PS-E68. ethanol and water extract were evaluated by phosphomolybdenum method (Prieto. 1999) and DPPH free radical scavenging capacity. The results showed that the total antioxidant capacity of PS extracted from Thuong hoang mushroom is lower than total antioxidant capacity of curcumin. but the DPPH free radical scavenging capacity of PS is significantly higher than that of curcumin. Structure features of polysaccharides of ethanol 48'fraction (PS-E48) were investigated by monosaccharide compositions and glycoside linked poshion analysis.
The results indicated that PS-E48 were composed of D-mannose, D-glucose, D-xylose. D- galactose. D-arabinose with molar ratios of 1.00: 0.29: 0.12: 0.08: 0 06. respectively. The PS-E48 had D-mannan framework which contained main linkages I—*6-D-mannoside, branches contained linkages such as I^f6~D-glucoside and l—t6-D-galacloside.
Keywords: antioxidant capacity. Phellinus linteus, polysaccharide structure.
