KHAO SAT HOAT TllMH KHAIXIG OXY HOA T U CAO CHIET ETHAIMOL LA, THAIXI V A VO TRAI CAY DUA (Ananas camasus) VUlXlii TAC CAU, KIEIXI GIAIXIG
Nguygn Trii Thu HiuS Trin Nhan Dang==, Hujnh Van B i ^ T6ng Van Hii^
T6MTAT
Dua CO t^n khoa hoc la Ananas comosus, la loai Uai cay co gia tn dinh duong cao. Khao sat kha nang khang . oxy hoa cua cao chiet la, than va vo trai dua dugc thuc hien nham tim ra nguOn nguyen li?u moi sit dung
trong nganh duoc lieu va my pham. Nghien ciru hieu suat ly trich cao Uong dung moi ethanol 96%, ty 1? phoi tron giua miu Oa - L_EtOH; than - T_EtOH va vo qua - V_EtOH) voi dung moi la 1:4, ket hop danh song sieu am voi cong suat la 120 Walt trong 48 gio. K6t qua cho thay. cao chi^t la, than, vo trai diia deu co ham lugng polyphenol tdng, nghidm thuc L_EtOH (206.1318 ± 0,021) va nghi?m thiic V_EtOH (249,709 ± 0,027 mg/g) cao hon nghiem thirc T_EtOH (159,519 ± 0,066 mg/g). Ngoai ra, hi$u suat trich cao thu dupe lon (doi vol mlu la 2,68% miu than 4,28 % va vo Uai 2,026%). Kha nang khang oiq' hoa DPPH. khir ion Fe^
thi nghiem thiic T_EtOH (567,1 + 28,75 pg/mL va 1983,482 ± 1,73 pg/mL) cao hon nghiem thiic L_EtOH (1190,6 ±106,03 Mg/mL va 6762,647 ± 659,12 ug/mL) va nghidm thiic V_EtOH (632,3 + 37.25 ng/mL va 3818,128 ± 27,13 iig/mL). Nghien cihi da phat hi^n viec tan dung ph^ pham tu la, than va vo qua diia co kha nang khang oxy hoa la nguon nguyen lieu tiem nang trong linh vuc san xusit dugc lieu.
Tir kh6a: Dua, ethanol khang oxy hoa, cao chidt, polyphenol
I.OATVAniDE
Dua CO ten khoa hoc li Ananas comosus la loai trii cay co gia tri dinh duong cao (Hassan etal., 2011;
Morton, 2007). Thit diia co ham luong acid hiiu co cao dac bi^t la acid Mahc, Citric, Fohc va Ascorbic (Ivanova etal., 2019). Mat khic, trong trai diiaco cic hgp chat co tic dung chdng tyrosinase, chong hyaluronidase, chong collagenase vi chdng elastase l i hgp chat Nanocomposite (Nd2Sn207-Sn02), hgp chat acid Ferulic (FA) vi p-coumanc acid (pCA) (Long et al., 2019; Wang et al., 2019; Zinatloo- Ajabshire/'a/,2019).
Trong CO the cua sinh vat (ke ca con nguoi) luon sin sinh ra cac goc tu do, la nhiing phan tir thuong CO mot hoac nhieu hon cic dien tu dpc than, dh phin ung voi cac chat khac din den su hinh thinh cac g6c tu do moi pha huy cac bao quan va cau true ben trong te bao din den dot bien va thoii hoa t^ bao (Hossain va Rahman, 2011; Pham Thi Be Tu va ShinkichiTawata,2015).
Su thoii hoa cua te bao (su oxy hoa te bao) la nguyen nhin chinh gay nen cac benh tat trong ca the
Truong Dai hgc Kien Giang
• Tnrong Dai hgc Can Tho
•' Truang Dai hgc Y Dugc Can Tho Email: [email protected]
con nguoi do tao ra qui nhieu phin ling chiia oxy (Reactive Oxygen Species-ROS (Hileman et al., 2004). Co the dgng vat va ca con ngiroi thirong tao ra cac hop chat co tinh khang oxy hoa. Khi ham lugng cic chit khang oxy hoa trong ca th^ giam xuong se lam tang nguy ca huy hoai cic te bio. Nhiing inh huong bat lgi cua ROS co th^ dupe ngan ngira bing each ho sung cac chat khing oxy hoa tir thuc phim, dupe Ueu (Conforti et al., 2008; Schramm et al., 2003). Cic hgp chat khang oxy hoa l i nhung hgp chat lam cham hoac ngan chan duoc su phit tri^n cua cic goc tu do bio ve te bao va ca the.
Dua Tic Cau thupc tinh Kien Giang tir lau da la dac sin noi tieng v i li cay nim trong danh sich dupe bio ton gen cua tinh Kien Giang ff)e in so 45/DA- SKHCN, ngiy 11 thing 4 nam 2014). Muc ti&u cua nghien ciiu nay li buoc diu dinh tinh cic hpp chat thien nhien va dinh gii kha nang khing oxy hoa tir li, than vi vo qui diia lam duoc lieu nhim tim ra ngu6n nguyen Ueu moi su dung trong nginh dtrpc Ueu v i my pham.
2. NQUyeU UBI VA PHUDNG PHAP 2.1. V§tU$u,h6a chit
Chat doi chimg; Acid Ascobic (99%. merck, Diic); acid gaUic (99%, merck. Nhat Ban).
Dung moi: Ethanol 96%. Methanol 96%. Vi$t N;im.
74 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 4/2020
KHOA HOC CdNG NGHE Hoa chat: DPPH (2,2- Diphenyl-lpiciylhydrazyl
(free radical), 95%), Hang Alfa Aesar, Nhat Bin;
FoUn-Ciocalteu, merck, Diic; NaaCOg 99,8%; Acid Ascobic 95%, ethanol 96%, methanol 96%, acid clohydric (36% HCl), acid sunfuric (HaSO^), nahi hidroxyde. FeCl3(95%), K3[Fe(CN)2], 99,5%, ethyl acetate, sodium acetat buffer (pH=5,5).
2.2. Ddi tupng nghifin ciiu
Li, thin vi vo qua diia (Ananas Comosus) duoc thu hii b viing Cu Lao, Tac Ciu, Kien Giang. Miu dupe dinh danh dua vio dac diem hinh thai theo Pham Hoing Hp (1993).
2.3. Fhuoog phip nghidn ciiu 2.3.1. Phuongphap thu miu
Thu la diia bit dau tir tang thii 3 - 6 (tinh tir gdc I6n) cua cay diia 3 - 5 nam tuoi (Hinh la), than diia (Hinh lb) v i vo diia (Hinh Ic) duac thu tai vi tri GPS (Vi do: 9,85826 B; Kinh dp: 105,13074 D). Thai gian thu miu tir 6-8 gio sing, ngiy 26/11/2019. Miu sau khi thu duac van chuydn v6 Phong thuc hanh sinh hoa, Tnrong Dai hpc Kien Giang, rua sach de kho tu nhien. Sau do, miu duoc cit nho voi kich thuoc 3 x 5 X 0,5 cm (rpng, dii, diy) va xu ly cac buoc tiep theo d^ tao cao chi^t.
Hinh 1. Li (a), thin (b) v i v6 diia {Ananas comosus^
vung Tic Ciu, Ki6n Giang 2.3.2. Phuongphap dieu chi cao (trich ly) Miu li, thin vi vo diia Tic Cau duge sau khi dugc lim sach, de kho tu nhien, cat nho va xac dinh khdi lugng tuoi. Sau do, say kho a 47''C den khdi lirgng khong doi, can lai de x i c dinh khdi lupng kho.
Tiep do, xay nho miu dong goi chan khong, bao quan b nhi^t dp -20''C, chuin hi cho cic cong doan hep theo.
1000 g m i u duoc ngam voi ethanol 96% (w/w) (EtOH), bing phuong phap ngam chiet voi ty le nguyen lieu/dung moi la 1:4 (w/v), nhiet dp trich ly
l i nhiet dp phong ket hgp danh song sieu am voi voi cong suit l i 120 Walt trong 48 gio. Sau do, lpc dich chi^t, lap lai 3 lan, dich chidt cua 3 lin dupe gom lai v i CO quay chin kh6ng de thu hoi dung moi duoi i p suat thap vi nhiet do 47"C (Balakrishnan va Kokilavani,2011).
Bing 1. Cic n g h i ^ thiic cao chi^t ethanol dua (Ananas comosus) viaig Tkc Ciu, Kifen Giang
Ten
nghiem Bp phan thiic
L_EtOH La Diia T_EtOH Than Diia V_EtOH Voqui
Diia
Dung moi Ethanol 96",
4000 mL Ethanol 96"
4000 mL Ethanol 96",
4000 mL
Xu ly song sieu am 120 wait, 60
phiit 120 wait, 60
phiit 120 wait, 60
phiit 2.3.3. Khao sat ham luang polyphenol tdng Ham lugng polyphenol duoc xac dinh dua tren phuong phip sii dung thudc thii Fohn-Ciocalteu. do quang pho b buoc song 765 nm. Chat chuan dupe su dung li acid galUc a 5 ndng dp 0,01; 0,05; 0,1; 0,25; 0,5 mg/mL. Ndng dp cao chidt su dung lan lupt la 0,1; 0,2;
0,3; 0,4; 0,5 m g / m L Him lupng polyphenol tong dupe tinh dua tren phuong trinh duong chuan y = ax + b cua chat chuan li acid galUc (Yadav vi Agarwala, 2011).
Him lupng polyphenol tong: C=cx Vm. Trong do C:
ham lupng polyphenol tdng (mg GAE/g chiet xuat); c:
gii tri X tir duong chuan voi acid gaUic/acid ascobic (pg/mL); V: the tich dich chife't (mL); M: khdi lupng cao chiet c6 trong thd tich V (g).
2.3.4. Phmmg phap khao sat kha nang khang oxy hda cua cao chidt
- Khio sat kha nang khang oxy hda bing DPPH Thi nghiem su dung DPPH ndng dp 0,5 mM pha trong methanol, sodium acetat buffer (pH - 5,5). Hoa tan cao chiet voi ndng do tir 0,1-0,5 mg/mL, acid ascobic ndng dp 0,01-0,05 mg/mL (diing lam dudng chuan). Do dp hap thu b buoc song 517 nm (Pham Thi Be Tu va Shinkichi Tawata, 2015). Mau ddi chiing dupe thuc hien tuong tu nhung thay the cao chiet hing MeOH. Thi nghiem duge lap lai 3 lan. Kha nang lie chd DPPH duoe tinh theo cong tiuic sau:
Phan tram irc che DPPH - - x l O O Trong do: A ; ^0 hap thu cua miu ddi chiing (khong chiia cao chiet); A do hip thu ciia miu. Xiy dung
duong chuan y = ax + b voi phan tram lie chd DPPH b cac ndng dp khic nhau. Tir do, tinh gia tri IC50 cua acid ascobic hay cao chiet
- Khio sit kha nang khing oxy hoa bang nang luc khirFe^^
Thi nghiem dupe thuc hien theo mo ta cua Singhal va cs., 2014, co hieu chinh. Acid ascobic cd ndng dp tir (0,5 - 3,5 pg/mL) dupe sir dung la chat chuan de so sanh vm cic nghiem thiic cao. Do budc song b do hip tiiu 700 nm, thi nghiem dugc lap lai 3 lin (Nguyen Van Ban etal, 2018). Kha nang khu ion Fe^* dupe tinh theo cong thiic:
A-Ao Khi nang khu (%) = - X l O O
nghiem tiuic L_EtOH (206,138 mg GAE/g cao hon 1 ^ lin so voi miu T_EtOH (159,519 mg GAE/g) va thap hon 1,2 lan so voi miu V_EtOH (249.709 mg GAE/g). Him lupng polyphenol cua nghiem tiiirc L_EtOH va nghiem thiic V_EtOH cao hon nghidm thiic T_EtOH khong nhieu do li vi vd trii l i nhirng bg phan tiep xiic true tiep voi moi tnrong va thuong xuyen chiu su chi phdi true tiep vdi m6i truang.
Bang 2. K^t qua dinh lupng h i m lugng polyphenol tdng
Trong do: A li d6 hip thu cua miu cao hoac Acid ascobic; Ao la dp hap phu cua mau tring.
2.4. Phucmg phip phin tich v i xu ly sd lieu Ket qua thuc nghiem dugc nhap lieu bing Microsoft Excel va phan tich bing phan mem MSTATC de phan tich phuong sai ANOVA, he sd bien d6ng (CV) va so sinh trung binh cic nghiem thiic bing kiem dinh LSD (0,05%).
3. KET QUA VA THAO LUAN
3.1. K^t qua dinh lugng h i m lugng polyphenol t^ng
Ham lirgTig polyphenol cua 2 nghiem fhiic dugc xac dinh dua hdn phirong trinh duong chuan acid galUc, bing cich the gii hi OD cua miu vio phuong trinh dudng chuan. Gii tri mg GAE/g chiet xuat cang cao thi ham luong chiing cd trong cao chiet cang cao v i ngupe lai.
Bang 2 cho thay, him lupTig polyphenol tdng trong miu than cay diia cao hon trong miu thit qui
Ten nghiem thiic L EtOH T EtOH V_EtOH
Ham luong irolyphenol (mg GAE/g chiet xuat)
206,138 + 0,020 159,519 ± 0,066 249,709 + 0,027 Nghien cuu niy mdi xac dinh ham lugng polyphenol tdng trong than, l i v i vo diia Tac Cau, Kien Giang. Tuy nhien, thanh phan cu the trong nhdm polyphenol tong moi quydt dinh hoat tinh sinh hpc, dac biet li hoat chat chdng oxy hda. Do dd, can tidn hanh nghien cuu siu hon vd him lugng flavonoid tdng (nhom chat cd vai trd tie che giai phdng cic chat dpc v i acid beo khong bao hda), him luong tannins, coumenns, quinones... Them vio dd, mdi nhdm chat cd vai trd quan trong trong te bio thuc vat giur nhi^m vu khic nhau nhu: tang tinh virng chic, tao miii huong, chat diet khuan va chat dgc voi con tning giy hai.... con ngirdi sii dung nhiing nhdm chat sinh hgc trong thuc vat vdi nhiing vai trd khic nhau nhir giim viem, chdng nhiem trimg, ha huydt ap va chdng oxy hda... Do dd, tuy vio muc dich ciia timg nghien am m i chiing ta cin di siu nghien cmi dinh tinh va dinh lupng cua timg nhdm chat hoac chat cu the.
3.2. Kdt qua khao s i t kha nang khing o:^ hda diia. Trong dd ham lupng polyphenol tong ciia
Bang 3. Phin ti^am lic chd ciia cao chidt li, thin v i vo qui diia vung T i c Ciu b ckc nong dO khic nhau thir nghiem bing phucmg phip bit gdc tif do DPPH
B6phan L EtOH T E t O H V EtOH
CV LSD 0,05%
N6ng do (ug/mU 100 (re/mL)
1,117' 5,008'- 16,504''
9,81%
3,328
200 (ug/mL) 4,972'' 9,872«
21,513''
300 (pg/mL) 9,585«
20,471'' 29,945'
400 (ug/mL) 13,476' 32,901'' 35,963'
500 (lig/mL) 19,639*
43,712"
40,576*
Ghi chii: Tmng ciing mot cdt. cac sd trung binh theo sau bed mot hoSc nhung chu cai gidng nhau thi khac biet khdng coy nghia thdng ke dmuv.y nghia 0,05% bang phep thir LSD
76 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 4/2020
KHOA HOC CdNG NGHE
Hoat tinh chdng oxy hoa cua cic cao chiet Ii, than va vo trii cay diia dugc dinh gii qua kha nang khu gdc tu do DPPH (2,2-diphenyl-l-picrylhydrazyl) vi k h i nang khu ion Fe^* thanh Fe^^. Phan tram lie chd cua cao chiet 6- miu li, than va vd qua diia Tic Ciu bing phuong phap DPPH dugc the hien qua bang 3.
Qua ket qui thd hien d' bing 3, o miu vo qua diia Tic Cau cd pham tram lie chd cao hon so vdi miu than vi miu li b ndng dp 100 - 400 pg/mL v i khong cd khic biet so vdi miu thin d' ndng dp 500 pg/mL.
Cu tiid, tai ndng dp 100 pg/mL, miu V_EtOH co phin tram ue che li 16,504%, cao hon 3,3 lan miu T_EtOH (5,008%) va cao hon 14,77 lan miu L_EtOH (1,117); tai nong dp 200 pg/mL, miu V_EtOH c6 phan tram lic che dat 21,513%, cao hon 2,18 lan mau T_EtOH (9,872%) va cao hon 4,33 lan so vdi miu L_EtOH (4,972%); tai ndng dp 300 pg/mL, miu V_EtOH cd phin tram lie chd deit 29,945%, cao hon 1,46 lin miu T_EtOH (20,471%) va cao hon 3,12 lan so voi miu L_EtOH (9,585%); tai ndng db 400 pg/mL, miu V_EtOH cd phan trSm tic che dat 35,963%, cao hon 1,09 lin miu T_EtOH (32,901%) va cao han 2,67 lan so vdi miu L_EtOH (13,476%); tai ndng dp 500 pg/mL, miu V_EtOH cd phan tram lic ehe dat 40,576%, cao hon 2,06 lin miu V_EtOH (19,639%) vi khac biet khflng cd y nghia so vcri miu T_EtOH (43,712%).
TIT ket qui phan tram tic che cua ba loai miu phd phim cua diia nhan thay, miu V_EtOH cd phan tram
lie chd eao hon miu T_EtOH v i miu L_EtOH khi d- ndng dp thap hon 400 pg/mL, khi ndng dp cao cua miu ti-ong khoang nay tiii b ndng do cing tiiap thi phan tram lic che cua miu V_EtOH cang cd sii khac biet so vdi 2 miu cdn lai. Ddng thoi, miu T_EtOH cd phan tram lic che tang nhanh hon 2 miu cdn lai khi gia tang ndng dp vi phin tram lic che ciia miu niy da cd su khic biet khong cd y nghia khi nong dp cao chiet tang len b 500 pg/mL.
Hossain vi Rahman (2011), da nghien ciiu kha nang khang oxy hda a i a tiiit qua dua khi trich vdi dung moi methanol, ethyl acetate vi nuoc, cho ket qua cao chiet cd kha nang khang oxy hda tren ba loai cao chidt theo thii tu tang dan la: metanol > ethyl acetate > chiet nude. Tuy nhien nhdm tic gia chua nghien ciiu kha nang khang oxy hda d thit qua diia voi cac bg phin khac Oi, than, vd qua).
Ket qua cua bang 3 v i bang 2 cho thay ham luong polyphenol (mg GAE/g chiet xuat) cd lien quan true tiep den phan tram lie che khi khao sat bing phuong phap khii gdc tu do DPPH eua miu V_EtOH so vdi miu T_EtOH v i miu L_EtOH. Him lupng polyphenol tong cang cao thi phan tram ue che cang lon din ddn kha nang lie che IC50 cang nhd va k h i nang khang oxy hda cing cao.
Khi nghien ciiu kha nang khir ion Fe^ thanh Fe^*, phin tram ue che ciia cao chiet o mau L_EtOH, T_EtOH va V_EtOH cua cay diia Tic Cau duge the hien qua bing 4 va hiiih 2.
Bing 4. Kdt qua phin tram lie chd DPPH ciia cao chidt L_EtOH, T_EtOH v i V_EtOH a cic ndng dO khic nhau thu nghidm bing phuong phip khii ion Fe^ thinh F e ^
Bpphan LEtOH T E t O H VEtOH CV LSD 0,05%
Nong do (ug/mL) 100 (yg/mL)
5,574' 5,093"
0,71°
2,61%
0,274
200 (ug/mL) 6,574«
7,398°
1,757'"
300 (ng/mL) 7,040"
9,155°
3,231"
400 (pg/mL) 7,399°
10,42' 3,952'
500 (pg/mL) 8,46ff"
11,130°
6,103*
Ghi chu: Trong cung mot cdt, cac sd trung binh theo sau bdi mot hoac nhung chu cai gidng nhau thi khac biit khdng cd y nghia thdng ke amuc y nghia 0,05% bkig phep thuLSD
Qua ket qua the hien a bang 4, xet tren cimg 1 ndng dp, phan tram lie ehe cua miu V_EtOH cho ket qua thap hon miu L_EtOH v i miu T_EtOH v i gia hi IC50 cua cic miu dtrpc tinh bing trung binh phan tram lie chd cua mau qua 3 lan lap lai tai cac ndng do khic nhau vi thd hien qua phuong trinh y=ax + b, khi
y=50 (Hinh 2).
Kha nang khang oxy ciia cao chiet dupe the liien qua gii tri 1C50, tai ndng do mau do tic che duoc 50%
gdc tu do. Gii tri IC50 cing thap miu se cd hoat tinh khang oxy hda cang cao va ngupe lai.
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Vitamin C
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N^d(>CHClMt(|l^W
Hinh 2. Phuong trinh dudng chulu cua vitamin C v^
cao chiAL_EtOH (a),T_EtOH (b) vaV_EtOH (c) trong thi nghidm kh^ng oxy hoa v6i kha n^ng khu
ionFe^
Ghi chu: Gia tri phan tram uc che la gia tri trung bmh cua ba lan lap lai.
I Brings. K^t qua khao s^t kha ndng khdng oxy h6a cua 3 nghifem thifc cap c h i ^ I, Ten nghiem
thuc
T_EtOH V_EtOH I Acid ascobic
IC50DPPH (ug/mL) 1190,6+106,03'
567,1 + 28,75' 632,25 + 37,25°
101,8 + 0,22''
IC50khus4t Ug/mL) 6763+ 659,12'"
3,482 ± 1,73"' 3818 +27,13' 11,014+ 0,47' Ghi chu: Trong cimg mot cot, cac sd dung binh theo sau bdi mot hoac nhung chu cai gidng nhau thi khac biet khong co y nghia thdng ke a muc y nghia 0,05%bingphep thuLSD
Ket qua tir bang 5 cho thay, ca ba mdu d^u co kha ndng khang oxy hoa. Mdu than cay dua Tdc Cau, Kien Giang Cr.EtOH) cho kha ndng khang oxy hoa tot hon miu la (L_EtOH) va mdu vo (V_EtOH) tren ca hai phuang phap thu kha ndng khang oxy hoa va ca ba mdu deu cho kha ndng khang did'p ban so voi vitamin C.
Dot voi kha ndng bdt goc tu do DPPH so sanh vdi ket qua cua Shete etal. (2015) nghien cihi tren la va cti cua hai loai cay Amorphophallus konkanensis va Amorphophallus bulbifer (ho Araceae) ly trich vol dung moi la ethanol 90% voi gia tri IC50 lan luot la 2700 pg/mL va 3020 pg/mL cho thay kha ndng khang oxy hoa cCia mSu la (1190,6 + 106,03), than (567,1 ± 28,75) va man vo qua (632,25 ± 37,25) ciia
cay diia vimg Tdc Can cho ket qua cao hon. Ket qua nay ciing cao hon nghien ciiu cOa Ngu\ en Vdn Bdn, et al. (2018), tren be cay mon ngiia ly trich bang ethanol 96% co ket hgp danh song sieu am trong 60 phut o 42''C CO kha ndng bdt gdc tu do DPPH voi IC50 la 1947 + 109,4 pg/mL. Them vao do, ket qua nghien ciiu tren la, than va vo qua diia viing Tdc Can cho thay kha ndng khang oxy hoa cao thong qua chi so 1C50 so voi nghien ciiu ciia Basu etal. (2012) tren d6i tuong cdy Colocasia esculenta, ly trich bdng phuong phdp Soxhlet va sir dung dung moi ethanol cho gia W IC50 la 1343,88 p g / m L
Khi so sanh giua ba nghiim thtic cao chiet voi nhau thi nghiem thiic cho gia IC50 thap nhat la T_EtOH aC50 - 567,1 + 28,75 ng/mL) d6ng nghia voi vi§c cho kha ndng khdng oxy hoa tot hon so voi nghiem thiic V_EtOH QCSO = 632,25 ± 37,25 ug/mL) vd mdu L.EtOH (1C50 = 1190,6 ± 106,03). Ndng luc khu cua cac hop chat thuc vat co boat tinh sinh hgc phan anh kha ndng khang oxy hoa Uiong qua kha ndng cho nang lugng dien tu (Koksal et al., 2008).
Cac hgp chat chong oxy hoa lam giam su hinh thanh sdt Fe^* thanh dang sdt Fe'* do kha ndng khii ctia chiing. Dg hap thu cang cao cho thay ndng luc khtr cang manh.
Doi vgi kha nang khir Fe^, khi so sanh giiia ba nghiem thtic cao chiet cung cho ket qua tuong hr vgi phuang phap bdt g6c tu do DPPH. Kha ndng khir Fe^* cang manh khi gid tri IC50 cang thap. Kdt qua cho fhay, gid hi IC50 thap nhat la mdu T_EtOH 0C50 - 1983,482 + 1,73 pg/mL) d6ng nghia voi viSc cho kha ndng khang oxy hoa tot ban so voi nghidm thiic V_EtOH (1C50 - 3818 ± 27,13 pg/mL) va mlu L_E10H (IC50 - 6763 + 659,12).
Nhu vay, khi sir dting hai phuong phap thir hoat tinh khang oxy khac nhau mac dii sd lieu cd sai khac nhung kdt qua thi gidng nhau vd kha ndng khang cxy hda di tir cao den thap theo thii tu cao chiet ethanol la:
than > vo > Id o cdy diia dupe trdng tai viing Tdc Cau, tinh Kien Giang.
4. KET LUAN
Cao chidt la, than va vd qua diia d Tdc Cau, Kien Giang cd ham lugng polyphenol tdng 6 cdc nghiem thiic cao chiet L_EtOH, T_EtOH va V_EtOH lan lugt la 206,1318 ± 0,021 mg GAE/g, 159,519 ± 0,066 mg/g va 249,709 + 0,027 mg GAE/g. Kha ndng khang oxy hda d nghiem thiic T_EtOH cao hon nghiem
78 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 4/2020
KHOA HOC C 6 N G NGHE
t h u c c a o c h i ^ t L _ E t O H v i n g h i e m thiic c a o c h i e t V ^ E t O H . N g h i e m thiic T _ E t O H , L . E t O H v i V _ E t O H cho IC50 voi D P P H v i khir s i t l i n l u o t la 567,1 + 28,75 v i 1983,482 + 1,73 p g / m L ; 1190,6 ±106,03 p g / m L v i 6762,647 ± 659,12 p g / m L v a 632,3 ± 37,25 p g / m L v i 3818,128 ± 27,13 p g / m L .
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A SURVEY ON ANTIOXIDANT ACTIVATION FROM ETHANOL EXTRACHON FROM -THE LEAVES, STEMS AND PEELS OF PINEAPPLE (Ananas comosu^ ATTAC CAU, KIEN GIANG REGION
Nguyen Thi Thu Hau^ Tran Nhan Dung^, Huynh Van Ba^ Tong Van Hai^
^Ken Giang University
^Can Tho University
^Can Tho University of Medicine and Pharmacy, Email: ntthau @vnkgu. edu. vn Summary
Pineapple is a fruit with very high nutritional value, its scientific name is Ananas comosus which cultivates in many different ecological regions in Vietnam. Although there have been a number of studies on chemical composition of edible part from pineapples but the use of pineapple waste products for medicinal materials purposes has not yet been addressed. A survey on oxidation properties from extracting leaves, stems and peels of pineapples was conducted In order to find whether it may a potential sources of matenals used in pharmaceutical and cosmetic industry. A study of extraction efficiency in 96% ethanol solvent, the mixing ratio between samples (leaves - L_EtOH; stems - T_EtOH and peeis - V__EtOH) and the solvent is 1 : 4, combined ultrasonic waves with a capacity of 120 Walt within 48 hours. The results showed that in the extract from leaves, stems and peels of pineapples contained the total polyphenol content of treatment L_EtOH (206.1318 + 0.021) and V_EtOH (249.709 ± 0.027 mg/g) is higher than T_EtOH (159 519 ± 0 066 mg/g). Besides, the obtained extraction efficiency is great (the sample of leaves is 2.68%, stems is 4,28% and peels is 1.137%). In terms of the DPPH oxidation resistance and deionized Fe3+, the treatment T_EtOH (567,1 ± 28 75 pg/mL and 1983.482 + 1,73 Mg/mL) was higher than the treatment L_EtOH (! 190.6 +106 03 (jg/mL and 6762.647 ± 659,12 Mg/mL) and the treatment V_EtOH (632.3 ± 37.25 |ig/mL and 3818.128 + 27,13 Mg/mL). The research suggestes that the pineapple waste products from leaves, stems and peels may used as an antioxidant properties as a potential source of materials in the field of pharmaceutical production
Keyword: Antioxidant, extraction, pineapple, polyphenol Ngudi phin b i ^ : GS.TS. Nguyen Cong Khin Ngiy nhin bii: 25/02/2020
Ngiy thdng qua phin bi^n: 26/3/2020 Ngiy duy$t dang: 02/4/2020
80 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 4/2020
