iinaiiei lal Province were inter\iewed. Descriptive statistieal tools and .Ajiova analysis were used to show hvelihi- livelihood resources, the \-ulnerabiht> of Uvelihood strategies, and financial efficiency. The research r. ' " " ' thai, in the present conditions, the household had abundant labor resources but the number of depen- , ^ "'^'i^
die difficulties in h ^ n g costs and education levels of the household member was low. However, in tei ,• • nauu.d capital, the area of ownership of the models varied considerably Financially, the diversity of bou.'-'-M'•'' iiuoine sources \% a,', not high Regarding social capital, the low participation rate of the association, it limiied . ischolds access to information. In terms of physical capital, most households satisfied with transportation, in ciH'^'^- """^
dykes. And among three main livelihood activities was having the statistically significant difterenees n efficiency and aquacultural production was a promising model for households income.
Key words: Climate change, \-ulnerability, flood area, livelihoods
Ngdy n h d n bdi: 29/4/2020 Ngutfi p h d n bien: PGS. TS. D d o T h e A n h Ngdy phdn bien: 13/5/2020 Ngdy duyet d a n g : 2 0 / 5 / 2 0 2 0
NGHIEN CLTU NUOI TAO Spirulinaplatensis BANG NLfdC THAI AO NUOI CA L O C (Chantia striata) VA ANH HUONG CUA MAT DO NUOI DEN SU* T A N G SINH KHOI
Dfltfng Hoang Oanh', Nguyen Thi Triic Linh'.
Nguyen Hodng Lam va Ph^m Kim Long' TOM
TAT
Bai viet trinh bJv nghien eflu u n dung nguon nUde thii ao nuoi cd Idc da xfl ly de nudi tdo Spirulina platensis.
Tbi nghii'm gdm eo 4 nghiem thflc, mdi nghiem thflc lap lai 3 lin. Nghiem thflc 1: mdi trfldng nfldc thdi ao nudi c i loc ed mdt dp lao ban dau la 1 - 10' tb/ml. (10%). Nghiem thflc 2: mdi trfldng nfldc thii ao nudi cd ldc cd mat d p tdo ban dau la I ..^ • 10' lb/mL(15%). Nghiem thfle 3 mdi trfldng nfldc thai ao nudi cd Idc cd mat d p tdo ban dau Id 2 ^ 10' ib/mL (20%). Nghiem thfle ddi ehflng: Mdi trfldng Zarrouk co mat dp t i o ban dau Id i » 10' tb/mL (10%).
Ket qud nghien eflu cho thay NIT dat mat dp dai cUe dai 52 681 ± 2 8 ! tb/mL d ngay nudi thfl 15, cd sinh khdi tdo thu dflpc 8.8f* .1 0,24g/L N 12 m i l dp dat cflc dai 54.134 + 489 tb/mL d ngay nudi thfl 13, cd sinh khdi t a o t h u dflpc 10,29 ± 0,10g/L. N T 3 m i t do dai cflc dai 54.617 ± 1.164 ib/mL d ngdy nudi thfl 11, cd sinh khdi tdo thu dflpc 10,6 ± 0,31g/L N T D C d.it m i l d p ciic dai ^-5 218 + 567 tb/mL d ngay nuoi thfl 16, co sinh khdi tdo thu dflpc 10,29 ± 0.29 g/L. Khi sfl dyng nfltfc thdi ao nudi cd loe d mat dp tdo ban dau 15 - 20% % dat sinh khdi tdo cao so vdi nudi tf mdt dp t i o ban dau 10% (p < 0.05). Hdm Iflong Protein eua ido ly le thuan vdi mat do nudi ban dau va ty 1^
nghjch vtfi ihdi gian nudi
Til khda: Spirulina platensis. Channa striata, nfldc thai nudi trong thiiy sin
I.DATVANDE
N g h e nudi e i ldc (Channa striata) t r o n g nhieu ed tdo xoan Spirulina (Le H o i n g Viet vd N g u y e n Vd n d m qua dd m a n g lai hi?u q u i kinh te c a o c h o n h i e u Chdu Ngdn, 2015). D o dd, nfldc nuoi cd ldc chi c a n n d n g ddn tinh Trd \ ' i n h , n h a t Id huy?n T r i Cfl. Tuy bd sung lflpng n h d k h o d n g chat cd it, j d u n g d e n h i e n , \nec m d r p n g dien tich nudi m d i each tfl phdt nudi sinh khdi tdo Spirulina platensis dai chat Iflpng k h d n g t h e o sfl k h u y e n c a o vd quy hoach cua ngdnh tdt. Ngoii ra, tan d u n g n g u d n n u d e thai tfl n u d i ca n d n g n g h i e p d i d a n d e n nguy cP l d n ve d n h i e m mdi lde se de nudi tdo Spirulina platensis se tiet kiem chi trfldng nfldc. Sfl gia tang vc dien tich nudi k e o t h e o phi, gidm gia thdnh sdn p h a m d d n g thui gdp p h a n sfl su)' gidm •Jfle chiu tdi cfla m d i trfldng. Nguy ctf d lam gidm dflpe d n h i e m m d i trfldng.
n h i e m hflu Ctf di?n r d n g d e d p a p h i vd n g h i e m t r o n g Tdo Spirulina sp. dflpc d u n g t r o n g xfl iy m d i h e sinh thai thuy sinh Trong khi dd, n g u d n d i n h t r u d n g nfldc va la thflc a n giau d m h dfldng dflpc dfldng tfl nfltfc thdi ed ldc dflpc d d n h g i i l i chfla rat cdc ddi t u p n g thfly s a n , gia sue va gia ...Vti ^fl j '^
n h i e u chat d i n h dfldng cd t h e 1dm phi dfldng cac vi Tan d u n g n g u o n nfldc t h i i a o n u d i ea !-, .g („•; ._^^^' t a o k h i d f l p c t b i i trflc tiep v i o m d i trfldng, t r o n g d d h i m lflpng d i n h dfldng d e nudi ta< -mtim!^"^
' TrUdng D41 hoc T r i \ ' i n h
Tap chi Khoan DC Co g - ; - , \zngnghi$pVie!Nam-So 5,; -4) XCC
nhim gdp phan giim thieu tinh trang d nhiem mdi
tn/dng cung nhfl mang 1^ hieu qua kinh te li viec can thiet nghien cfla Nghien cflu cilia Nguyen Thi Thanh Nhi^n (2010) sii dung nfldc thai ao nudi ci tre de nudi tio, ket qui cho thay d nghiem thflc sfl dyng 100% mdi trUdng nfldc thai dat mat dp cao nhat d ngiy thfl 5, cac nghiem thflc sfl dung mdi trfldng nfldc thdi hi^u qua thap htfn nghiem thflc sii dung mdi trUdng Zarrouk. Nghien cflu cua Ngd Thuy Thuy Tam (2009) cho thay mat dp nudi thich hpp cho sfl phit trien cua tdo li 30.000 tb/mL sau 15 ngiy nudi vi ty 1? thu sinh khdi 25%/ngiy se dflpc sfl dung de nudi vdi be cd the tich ldn htfn.
Tuy nhien, eac nghien eflu tren cung chi dflng lai d mflc dp danh gid bieu qua sfl dung nUdc thai de nudi tio eung nhfl sfl phal trien cua tio Spirulina
platensis nhUng chfla nghien cflu mdi tflpng quangifla sfl gia ting sinh khoi tao trong mdi trUdng nfldc thai vdi ty Ie cap gidng ban dau nham thu dUpc sinh khdi tio cao nhat. Bai bio niy trinh bay ket qua nghien cflu nudi tao Spirulina platensis bing nfldc thai ao nudi cd ldc va dnh hfldng cua mat dp nudi den sfl tang sinh khdi,
IL \'AT Ll£U VA PHUONG P H A P N G H I £ N CL-l'
2.1. Vat lieu nghien ciiu
Bang l.\'at heu nghien cflu Diing cu vi thiet hi thi nghiem
Lam + Lamel Binh djnh mflc Cdc thfly tinh Micropipet, dau col Binh xit cdn Den cdn, cdn 70", 90"
Pipetpaster Binh tam giac 1 lit H^ thdng syc khi 2.2. Phflpng phap b6 tri thi nghiem
Thi nghiem cd 4 nghiem thflc: Nghiem thflc 1 mdt dp tio cay la 10% (1«10' tb/mL); Nghiem thflc 2:
mdt dp tio cay la 15% (1,5 ^10-'tb/mL); Nghiem thflc3:
mdt dp tio cay la 20% (2 x 10"' tb/mL); Nghiem thflc ddi chflng: mat dp tao cay 10% (1 x 10* tb/mL).
Thi nghiem dflpc bd tri trong binh tam gidc cd the tich 1 lit, mdi nghiem thflc lap lai 3 lan. Tien hanh nudi vdi cfldng do anh sang: 2.500 lux, chieu sang 12/24, mdi trfldng dinh dfldng cho tdo phdt trien dil0c cung cap vdo ngdy dau tien bd tri thi nghiem, sue khi lien tyc trong suot qud trinh nudi.
Mdi trfldng nudi cay va dung cu nudi cay dUpc hap khii trflng bing ndi hap tiet trung d 12rC trong 15 phflt. Theo ddi cac yeu to mdi trfldng va mdt dp tao hang ngdy
Xfl ly nfldc thai ao nudi ca ldc do nfldc thai tfl ao nudi cd chfla thanh phan can IP Iflng cao vi viy nfldc thdi cho qua he thong Ipc thd de xif ly thanh phan can Itf ldng. Sau khi da xu ly cac thanh phan
Dung cu vi thiet bi thi nghiem Kinh hien vi
NOI hap lift trflng Budng dem Sedgwick-Rafter Miy do cfldng dp inh sang
•May do pH (WTW pHoto Flex) Cdn dien lfl 4 so le + Giay bac Bep dun + ei lfl Mdi trfldng dinh dfldng Den huynh quang
can iP Iflng, thinh phan hflu cp khd phan huy dflpc xfl ly tiep bing phfltfng phdp ozon xuc tdc (sue ozon) vd ddng thdi di?t khuan cho nUdc. Sau dd, nfldc thai dfltfc cho chay qua cac cdt lpc tinh kich thfldc 1 micron, muc dich Id de loai bd cdc chat can cdn sdt cua he thong lpc thd, dieu ndy giflp cho ngudn nfldc sach htfn vd cudi cung nfldc thii dflpc cho vdo xd chfla vd sue ozon mpt tan nfla trfldc khi dfla vdo sfl dung.
Nfldc thai ao nudi cd ldc sau xfl ly cd gid trj pH bing 7,5 vd nitrat dat 8,8 mg/L, cdc yeu td nhfl TSS, amonium khdng phit hien. Ngoii ra, nfldc nay cdn chfla nhieu khoang chat nhfl Mg^% Ca'". Na', SO^^
Cl, Fe-". Nhflng khoang chat niy la ngudn dinh dfldng quan trpng trong viec nudi tio Spirulina
platensis.Tdo Spirulina platensis dUtfc thflc hien nudi trong
mdi trUdng nfldc thii ao nudi ca ldc sau khi d i xtf
ly vi bo sung them thinh phan dinh dfldng theo
bang 2.
et Nam-So Sir J :ojo Bang 2. Cae thanh phdn hoa hoc bd sung vao mdi IrUdng nfloe thdi ea loc so voi mdi trfldng Zarrouk
.Mdi trfldng nfldc thai c i ldc bd sung d i n h dfldng
Mdi trfldng Z a r r o u k ( N T D C )
Jhdnh phdn Lieu pha ^ . , . . Tieu pha , , ; , Thanh phan (g/L) *••"" (g/L) EDTA 0.012 EDTA 0,08 N J N O 0.375 NaNO, 2,5 FeS0,.7H,0 0.0015 FeSO,,7H O 0,01 NaHCl), 2,52 N J H C O 16,8
K.HPO 0,5 K,SO, 1.0 NaC! 1,0 MgSO,:H(l 0,2 CJCI 211 O 0,04 2.2.2. Cdc chi tieu theo ddi
a) Vieo doi cue yi'u id nun trUdng trong qud Irinh nghien cuu
Kiem tra p H 2 lan/ngay Iflc sang: 8 h; chieu. 2 h Kiem tra nhi^t d p nfldc 2 l i n / n g i y luc sang: S h.
chieu: 2 h.
b) PhUdngphdp \ac dinh smh khoi tdo - Xdc dinh thdi diem thu tao: thu lao Iflc 8 h sang.
Phfltfng p h i p xdc d i n h m a t d o ido: D u n g M i e r o p i p e p ed t h e tich 1 ml h u t t i o tfl t r o n g binh l a m gidc vdo d n g ly l a m e d the lieh 10 niL. lien h a n h p h a loang bdc 5 (1 luan d a u tien) vd p h a loang bae 10 (ludn tiep t h e o ) , sau d d l i c d e u vd hut 1 mL d i p h a l o i n g vdo b u d n g d e m Sedgwick-Rafter co t h e lich I m L , dd\ lamel lai vd tien h d n h d e m tdo dai dien tren 125 d (25 d/gdc: 4 gdc vd 1 gifla) d vdt k i n h 10, ldp lai 3 ldn d e m .
- C a e h tinh mat d p tao:
Sd Ifltfng t i o = T x (.A N) » \ ' p h a loang Trong do: T: tong sd te biw di'm diidc: A: tong so o cua hudng dem: N. long so d dem duoc: V pha lodng the lich pha lodng.
The tich b u d n g d e m : 1,0 m L . Budng d e m tdo Sedgwick Rafter.
X.ie d m h s i n h k h d i l a o s a u k h i ket t h u c t h i n g h i e m : .Xae d j n h t h d i gian t a o d a t cue d a i , sau 1 d e n 2 ngdv tien h d n h t h u h o a c h l i o bdng Ifltfi loe
ICI 11
:h Jn
nuhiui CO kich Ihfltfc mde Ifldi Id 5 - 10 mi.
c i n sinh khdi t i o tfloi b i n g cdn 4 .so le lflpng t i o d cac n g h i e m thflc t h i nghi.
thflc d d i chflng. M i u l i o d d sdy k h d tieu p r o t e i n t h d ( d a m ) .
c) PhUdngphdp phdn tich Protein cuu . <' -V platensis
Protein: Theo phflong phdp TC\'N 432S-1 2007.
2.2.3. PhUdngphdp xd ly so lieu
Cdc sd lieu dflpc phdn tich bing phdn mem Excel 2010 vd SPSS 20.0 Analyze, Compare Means, Oneway Anova, Post Hoc Multiple Comparisons vdi phep kiem dmh Duncan's Test vd Tuke\ Test dflpc sfl dung de xic dinh sfl khic biet cd y nghia thdng ke vdi mflc y nghia p < 0,05. Tat ci cdc sd li^u trong tbi nghiem dflpc trinh bay dudi dang trung binh (Mean) ± do lech chuan chuan (SD).
2.3. Tiidi gian va dia die'm nghien ciiiu Nghien cflu dfltfc thfle hien tfl thing 1/2018 den 11/2018, tai Khoa Nong nghiep - Tliuy san, Trfldng Dai hoe Trd Vinh.
III. KET QUA \A THAO 1 UAN
3.1. Bien ddng mdi trUdng cP ban trong qua trinh nudi tio
3.7./. YeutopH
Ket qud h i n h I c h o thay p H 6 c a c n g h i e m thflc Irong q u i trinh n u d i cd gid t r i t r u n g b i n h lan Iflpt:
N TDC: 9,60 ± 0,02. N T l 9,58 ± 0,04, NT2- 9,55 ± 0,03, N 1 3' 9,64 ± 0,03. p H cfla m o i n g h i e m thflc d a t gid tri eao nhal d nhflng ngdy n u d i k h i c n h a u , c u t h e N T D C : 10,04 ± 0,02 d ngay n u d i thfl 16, N T l : 10,48
± 0,07 d ngay nudi thfl ] 5, N T 2 : 10,49 ± 0,05 d n g i y nudi thfl 13, N T 3 : 10,39 ± 0,05 d ngay nudi thfl 11.
^ Sd di NT3 dat gia tri p H c a o d ngay i hfl 1] |a d o mdt d p tdo ban ddu dflpc b d t r i cao d a n d e n sfl p h a t t n e n cfla tdo dien ra n h a n h hOn eac n g h i e m thflc eon lai. N h i n c h u n g , k h o i n g d a o d d n g pH cfla c a c nghiem thfle p h u htfp vdi ket l u i n c u a lru(,ng Van Lung (2004) e h o rang tdo Spirulina sdng trong m d i irutfng giau HCC) , p H lfl 8.3 - 10,1 v., D^ai^ - ^ j O a n h (2019) c h o rang p H c h o tdo Spiruhna platen-^
p h i t t n e n tfl H,5 - 9,5.
ng nghiep Vie! Nam • i d •
-NTDC (10%) - N T l (10%) - N T 2 ( 1 5 % ) - N T 3 (20%)
3 4 5 6 7 S 9 10 11 12 13 U 15 16 17
Hinh 1, Bieu dd the hien gia trj pH trung binh hdng ngiy
3.1.2. Nhiet doHinh 2 cho thay nhiet dp d cic nghiem thflc trong qui trinh nudi dat gid tri trung binh lan Iflpt: NTDC.
30,46 ± 0,04, NTl: 30,35 ± 0,3. NT2: 30,30 ± 0,21, NT3:30,37 ± 0,3. Nhiet dp cua cac nghiem thflc tang tuong ddi dn dinh trong nhflng ngay ddu. Tuy nhien vdo ngdy thfl 11, 12 vd 15, 16 cua thi nghiem d cdc nghiem thflc cd sfl gidm nhiet dp nhflng chech lech
khdng qud 2'C do trong qua trinh bd tri thi nghiem thdi net ben ngodi khdng on dinh, xdy ra nhflng Iran mfla bit thfldng nhflng khdng lim inh hfldng den sfl phdt trien cfla tdo. Kel qua niy phfl hpp vdi nghien cflu cfla Vonskhak vi Tomaselli (2000) cho ring cd nhieu lodi Spirulina thich hpp sinh trfldng d khoang nhiet dd tfl 24 - 42"C.
27.0 25.0
V"**^V^^y?^
-NTDC- N T l lOH -NT2 15H -NT2:0'!o 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
Ngiy Hinh 2. Bieu dd the hien bien ddng nhiet dd trung binh hang ngay 3.2. Di^n bien mat dp tao trong qua trinh thi nghiem
Qua ket qua d bang 3 cho thay mat dd tan d cdc Id ihi nghiem cd sfl phal trien tdng dan deu theo thdi gian. Gia tri ^'e mat dp eua cdc thi nghiem tudn theo qui ludt, cu the d NT3 (20"n) mat dp tdo dat cflc dai 54.617 ± 1.164 ib/niL d ngdy nudi thfl 11.
NT2 (15%) mat dp lao dat cflc dai 54.134 ± 489 tb/mL d ngdy nudi thfl 13. NTl (10%) mat dd tao dat cflc dai 52.681 ± 281 tb/mL d ngdv nudi thfl 15.
NTDC mat dp tio dat cflc dai 54.218 ± 567 tb/mL d ngdy nudi thfl 16. Sd di, mat dp tao d NTDC vd N'TI chu ki sinh trfldng phdt tnen chdm htfn vd keo ddi hon Id do mat dd tdo ban ddu thap hPn so vpi N*r2 vd NT3. Iheo Le Van Cdt (2006), tdo Spirulina khi dat mat dp cao se che chan bdt dnh sang vd qud trinh quang htfp se kem di ddng thdi kim ham sfl phat trien tiep theo cua tdo. Dieu nay phu hpp vdi sfl phit tnen cua NT3, tao dat mat dp ciic dai d ngdy nudi thfl 11 vd tan lui d cdc ngay sau dd. Sfl phdt trien
cflc dai liep theo d NT2 Id ngay nudi Ihfl 13 vd tiep tuc lan lui cdc ngdy sau dd, d NTl vd NTDC tdo dat mat dd cflc dai d ngdy nudi thfl 15 vd 16.
d NTDC vd NTl dflpc nudi vdi mat dp ban dau li 10% nhflng ket qud NTl dflpc nudi tfl nfldc nudi cd ldc dat mat dd tdi flu trfldc NTDC 1 ngdy nhflng mat dd cung khdng dat bang NTDC. Cf NT2 vd NTS tuy mat dp bd tri ban ddu khac nhau nhflng tfl ngdy nudi thfl 7 - 10 cd 2 nghiem thflc khdng cd sfl khdc biet thdng ke (p < 0,05). Tfl ngiy thfl 11, mat dd tao tf NT3 dai cflc dai vd cd sfl khic biet ihong ke so vdi NT2 vd giim mit dp dan d cdc ngay tiep theo.
Trong khi dd, NT3 dat mdt dp tdo cflc dai d ngiy
thfl 13 (54.134 ± 489 tb/mL) vd mat dp nay cung
tflpng ddng so vdi mat dd tdo d NT3 ngdy thfl 11
(54.617 ± 1.164 tb/mL). Dieu ndy cho thay, khi nudi
tio Spirulina plalensis vdi ty le mat dp tio ban dau
20% thdi gian nudi ngan nhat.
Nsa>
17 Chl,
Bang 3
STDC 10.000 ± 0 14 769 ± 154 15 7,S- t 284' 1 , S 5 4 7 ± : 9 ; ' 20.253 ± 528' 23 471 ± 2 740"
29 7 7 » r 2 7 W 35.662 ± 1.946' 38.3.S7t 2.126' 40.027 ± 2.486' 41.236 ± .375' 43 502 *659' 4 6 . 2 I 8 ± 1 I57>
47.S78 1 1 957"
50.556 ± 1.314- .54 21H±56'c 5041.S ± 2 147"
T i n j ; : : .iiini; \c ni.,1 J o Ngh . \ T I (IO'„J
10.000 ± 0 13.697 ±77.3' 17.324 = 5 9 r 22.520 : "0,«- 25.8.57 ± 378"
29.8.53 ± 7 2 1 "
34 000 ±.54 r 37 4S0 z 488- 40.955 ± 558*
42.382 ± 8 1 8 ' 45 146 ± 1.064' 4(, 3,17 t 1 223'
47 997 ± 7"8 51.053 ±543- 52.681 ± 28 d 47 947 ± 690' 0 ± 0 '
ua 1,10 a cac nghiem thuc em thiic
NT2 115%) 15.000 ± 0 17.528 ± 20"
20.466 ± 843"
25.062 ± 62"
28.881 ± 6 4 8 "
34.840 ± 209' 38.826 ± 995' 41.102 ±1.199' 43.395 ± 1.062'"
46.560 ± 6 1 2 ' 50.533 ± 323"
52.467 ± 1 9 7 ' j 54.134 ± 489' 48.680 ± 6 2 5 ' 44.008 ± 1 7 4 0 ' '
0 ± 0 "
0 ± 0 '
Si ,"
20 Olio ^ ' 23 5 ; ; 110S-' 2 S 0 ' , i tSOS-' 29 "S t t 569-' 35 03 "• ± 603'' 37.561 ± 182-' 40 5,10 ± I ' l l ' 4 3 . 0 s ; ± 745' 44.01,; ± 1.681' 49,324 1 1.539' 54.617 ± 1.164-1 45.187 ± 2.679- 41.613 ± 2.147' 0 ± 0 ' 0 ± 0 ' 0 ± 0 ' 0 ± 0 "
-lui- So liia Irinh hay Irang hdng Id gui In Irung binh ± do lech chuan (SD) (Ib/mL). Cdc gid tri trong cimg mot
tac ,lid cdi khdc nhau Id khdc nhau ^dy nghia thong kc (p < 0.05).3,3, Sinh khoi tao thu hoach cl cac nghiem thilc Ngav CUOI ciing ket thuc vice xac dinh mat do tao ciia cic nglii,'ni thilc (Bang 3). Tien hanh thu sinh khoi tdo ciing ng^y va co ket qua nhii bang 4.
Ket qua bang 4 cho thay \ 7 3 dat sinh khoi cao nhat (10,6 ± 0,31 g/L) ke den la NTDC dat sinh khoi
(10,29 ± 0,29 g/L), ke- do la NT2 (10,29 ± 0,10 g/L), thap nhat la NTl (8,88 ± 0,24 g/L). Ca 3 nghiem thflc (NTDC, NT2, NTS) deu khong co khac biet y nghia thong ke vdi nhau nhflng ca 3 nghiem thflc NT lai c6 stJ khac biet thong ke doi vcfi NTl.
Nghiem thflc Sinh Ivhoi lao
Bang 4. Sinh kh NTDC
(ngay thu 17) 10,29 - 0.2 b
Ihu hoach (g/L)
NTl NT2 (ngay thu 16) (ngay thu 15)
8,88±0,2*a_ 10,29 ± 0 , r b
NT3 (ngay thu 13)
10,6±0,3'b
Ghi chu. Soliculrinh ba, tnnig /„i„s lag,., ,r,:n.,,g bmh ± do lich chudn (SD) (g/L). Cdc gid tn irong ci,ng mot luiiigcocac ,huiai khac nhattklhw iib.iu CO, ilC'H'i'-'lor.'C KC (p < 0,05). 's "i"^3.4. Ket qua phan tich ham Iflong protein Ket qua phan tich ham lfldng protein qua bang 5 cho thay ham Ifl<?ng protein ca 4 nghiem thflc dat tfl 65,40 - 68.89'^,, Trong do, ham Iflong protein a NT3 d^t chi so cao ban NT2, XTI va NTDC. Tu\
nhien, ket qua nay hoan toan phu h(?p vdi nghien cflu cfla Belay (2002) va Tran Thj Le Trang (2013), tao Spirulina plalensis la mpt loai tao lam co gia tri dinh dfldng rat cao, vdi ham Iflpng protein chiem tdi 56 - 77% khoi Ifldng khd. Mat khac, theo thfl tfl
NTDC, NTl, NT2 va NT3 cd ham lupng p„tein lang dan va ty Ie nghich vdi thdi gian nuoi cua cac nghiem thflc nay. Theo Vd Hong Tru,,,, « cong tac vien (2017) xac dinh khi nuoi ta
' gian cang dai ham luong protein cang giam va ham lupne carbohydrate cang cao. Nghien cflu cho thay „ ^ ^ , tao trong moi trudng nfldc thai ao nuo, ci lie dat ham Iflpng prulein cao hon moi truon • .',,rroi,l, "•
nu6i tao d mat do ban dau cang ca<, s,', ,,„ ™
cang giam thi ham Iflpng protein dat cao "'"
Tap chl Khoa hoc Cong - j -, Nong nghiep Vii.
Bing 5. Kft qud phin tich him lUpng protein cua tdo Spirulina platensis
NTDC N T T ~ \ T 2 NT3 (ngaythu 17) (ngdythu 16) (^d) thu 15[ (ngdythuU) Chi s6 protein tho 65,40% 66.45% 68,12% 6S.S9'V
Nghiem thflc
IV. KET LUAN VA DE NGHI 4.1. Kel luan
Nfldc thai ao nudi ca ldc cd the sfl dung de nudi tio Spirulina platensis.
Nudi tdo d mat dp tio ban dau 15 - 20% % dat sinh khdi lio cao so vdi nudi d mat do tdo ban dau 10%.
Him lupng Protein cua tdo ty le thudn vdi mat dp nudi ban dau vd ty le nghich vdi thdi gian nudi.
4.2. De nghi
Nghien cflu them ve dnh hfldng cua cfldng do dnh sdng ldn hPn 2500 lux vdi thdi gian chieu sdng ddi hpn 12/24 h de rflt ngdn dflpc thdi gian nudi.
TAILIEUTHAMKHAO
Le Van Cdt. 2006. NUdc nuoi thdy sdn chdt lUdng vd gidi phdpidi thien chdt lUdng. Nhd xual bin Khoa hpc va Ky thudt, Hi Npi, 424 trang.
TrUdng Vin Lung, 2004. Cong nghe sinh hoc mgt so lodi tdo kinh te Nhd xual bin Khoa hpc ky thual. Hi Ndi, 141 trang.
NguySn Thi Thanh Nhiln, 2010. SU dung nUdc thai ao nuoi cd lii- de nuoi Ido Spiruhna trong phong thi nghiem vdo miia mUa. Ludn van lot nghiep dai hpe trfldng Dai hpc Can Thtf.
Dodn Thi Oanh, 2019, Nghien eflu lam sach CO, tfl khi thii dot than bdng ky thuat xuc tdc - hap phu de Idm ngudn cacbon nudi vi khuan lam Spirulina plalensis giiu dmh dfldng.
Ngd Thuy Thuy Tam, 2009. Phdt trien nuoi sinh kiwi Ido Spiruhna plalensis trong phdng thi nghiem. Ludn vin tdt nghiep dai hpe Trfldng Dai hpc Can Thtf.
Tran Thi Le Trang. 2013. Anh hfldng cua cac mflc nittf khac nhau le sinh trfldng, ham Iflpng protein vd lipid cua tio Spiruhna platensis (Geitler, 1925) nudi trong nfldc man. Tap chi Khoa hoc - TrUdng Dai hoc Can Thd. Phan B, sd 26 trang 180-187.
Vd Hong Trung, Nguyin Thi Bich Ngpc, Train Huynh Phong, Nguyen Thi Hong Phiic, 2017. Anh hudng cua ehdt Iflpng anh sing Ien sfl tang trfldng, ham lupng carbohydrate vd protein d Spirulina sp. Tap chi Khoa hoc - TrUdng Dai hgc sU pham thdnh pho H6 Chi .\hnli, lap 14. so 12. trang 117-126.
Le Hoing Viet vi Nguyin Vo Chiu Ngdn, 2017 Xfl ly nfldc thdi lfl him ii biogas bing ao thim eanh tdo Spirulina sp. Tap chi Khoa hoc - TrUdng Dai hgc Cdn T^id.Sd 49a. trang 1-10
Tieu chuan Viet Nam TCVN 4328-1:2007 (ISO 5983- 1:2005) ve thflc an chin nudi - Xie djnh ham Iflpng Nittf vd tinh ham Iflpng protein thd - Phfltfng phap Kjeidahl.
Belay A., Kato, T., Ota. 2002. The Potential Application of Spiruhna (Arthrospira) as a Nutritional and Therapeutic Supplement in Health Management. Ihe Journal of the American Nutraceutical Association Vol. 5, No 2.
Vonshak and Tomaselli, 2000. Arthrospira (Spiruhna):
systematics and eeophysiology. In: Whitton, B.A , Polls, M. (Eds,), Ecology of Cyanobacleria, Kluwer.
The Netherlands, pp. 505-523.
Study on Spirulina platensis culture by using treated wastewater from snake head (Channa striata) and effect of algae density on biomass
The .study aimed to use treated wastewater from snakehead pond for micro algae Spirulina plalensis cukiire. The experiment consisted of 4 treatments, which used the same source of treated wastewater from snake head fish pond,.Each experiment was replicated 3 times. The first treatment was tested with an initial density of 1 » 10*cells/inL (10%) of Spirulina platensis. The density of Spirulina platensis in second and third experiment was 1,5 >< 10* cells/
mL (15%) and 2 « IO''cells/ml. (20%), respectively. The Control treatment used standard Zarrouk environment with the initial density of Spirulina plalensis at 1 " lO^'cells/mL (10%). The results showed that treatment 1 reached the maximum density of 52,681 +281 cells/mL within 15 days of culture, with the biomass weight of 8.88 ± 0.24 g/L Tn treatment 2, (15% initial algae cells), il reached the maximum density of 54,134 ± 489 cells/mL within 13 days, with obtained algae biomass weight of 10.29 ±0.10 g/L. In treatment 3,(20% initial algae cells), it reached the Uiaximum density of 54,617 ± 1,164 ceils/mL only within 11 days (biomass weight of 10.6 ± 0.31 g/L). The control treatment reached the highest density of 54,218 ± 567 cells/mL al the 16"'day, with tbebiomass weight of 10.29 ± 0.29 g/L Keywords: Spiruhna platensis, Channa striata, aquaculture wastewater
Ngay nhin bai: 20/4/2020 Ngiy phin bien: 11/5/2020
Ngfldi phdn bi^n: TS. Pham Thii Giang Ngiy duyet dang: 20/5/2020
