Nghien cHru YDHCT Viet Nam So 40 - 2014
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B u ' d c D A U D A N H G I A T A C D U N G C H O N G O X Y H O A C U A PHU*GfNG P H A P L U Y E N T A P DU*OfNG S I N H N G U Y E N V A N HU^CRVGT R E N B E N H N H A N B E O P H I
E v a l u a t i o n on t h e a n t i o x i d a n t effects of N g u y e n V a n H u o n g m e t h o d on obese p a t i e n t s
* Nguyen Thj Bich Hong ', * Nguyen Van Turdug^,
** Do Thi Phu-omg ^ *** Nguyen Thj Van Anh' ' Binh viin Y hoc co truyen Trung ucmg. ^ Khoa YHCT Truang Dgi hoc Y Hd Noi, Tom Tat:
Bio phi la benh khd pho bien tren the gidi vd Viet Nam. Beo phi gidi phong ra nhieu cdc goc tif do. Mue tieu nghien citu: "Ddnh gid tdc dung chong oxy hoa cua phucmg phdp luyen tdp duong sinh Nguyen Van Huong tren benh nhdn beo phi". Phuong phdp nghien cuu: tiin cuu, so sdnfi trudc sau vd so sdnh voi nhom chung. 60 doi tuang beo phi du(rc chia Idm hai nhom: nhom nghiin cuu (30 ddi tuang) tap theo phuang phdp duang sinh Nguyen Vdn Hucmg, nhom chdng (30 ddi tirang) tap theo phuang phdp Hurbbard. Thdi gian luyen tap 15 ngdy. Ket qua: ndng do enzym chong oxy hoa SOD,p, TAS, GPx.p, GPxhc diu tdng sau 15ngdyluy$n tap vdip< 0,0] -0,001 dca hai nhom. Kit iuan: Luyen tap dudng sinh theo phucmg phdp Nguyin Vdn Hudng cd tdc dung Idm tdng cdc chdt chdng oxy hoa tren benh nhdn beo phi
Tur khoa: Beo phi, chSt chong oxy hoa, phironng phap diro-ng sinh Nguyen V3n Hirong, phiTffng phap Hurbard.
Summary
E V A L U A T I O N O N T H E A N T I O X I D A N T E F F E C T O F N G U Y E N V A N H U O N G M E T H O D O N O B E S E P A T I E N T S Obesity is the most common disease in the world and in Vietnam. Obesity releases many free radicals Objectives: To evaluate the antioxidant effect of Nguyen Van Huong method on obese patient. Methods: Prospective clinical trial, comparison ofpre-post treatment and control group.
60 obese patients were divided into two groups • the research group (30 patients) practiced Nguyen Van Huong method and the control group (30 patients) practiced Hurbbard method. The practice time had lasted for 15 days. Results: The concentration of antioxidant enzymes SODtp, TAS, GPx was increased after 15 days with p<0 001- 0.0! in both groups. Conclusion: Practicing Nguyen Van Huong method increases antioxidants in obese patients.
Keywords: Obesity, antioxidants, Nguyen Van Huong method, Hurbard method.
Dia chi lien he: Nguyin Thi Van Anh - Benh vien Y hoc c6 truyfin TW Ngay nhain b i i : 11/02/2014 Dja chi email: [email protected] Ngiy p h i n bi^n: 12/02/2014 Phan bien khoa hoc: PGS.TS. Nguyin Nhiroc Kim Ngay dang bao- 24/-;/9ni4
* ThS.BS; ** PGS.TS; *** TS.BS
Journal of research in viet nam traditional medicine and pharmacy I. DAT VAN DE
Beo phi duac coi la benh ly man tinh, ciing nhu cac benh man tinh khac, beo phi gay ra cac qua trinh oxy hoa, giai phong ra nhieu cac g6c tu do [1].[2]. Khi beo phi t6n tai trong mpt thofi gian dai, ngu6n chat chong oxy hoa trong co the bi can kiet, giam nong dp va boat dp cac enzym nhu SOD, TAS, GPx, so vai nguai khoe manh.
Cac goc tir do se tao ra hang loat phan ling bat loi cho co the [3]. Ben canh cac phuong phap dieu tri beo phi ciia YHHD, thi YHCT ciing co nhieu phirong phap dieu tri nhu phuong phap diing thuoc va khong dung thuoc da dupc ap dung tir lau. Phuong phap luyen tap duong sinh Nguyen Van Huong [4] da dupc ap dung 6 BV YHCT TW tir nam 1957 din nay. Do la phuang phap luyen tap tong hap, la sir ke thiia nhung kinh nghiem quy bau ciia 6ng cha ta de lai va nhung tinh hoa cua y hpc phuang D6ng . Tren thuc te lam sang va nhieu cong trinh nghien ciiu da chiing minh tac dung cua phuang phap nay [6], [7], [8],... Tuy nhien chua co cong trinh nghien ciiu nao danh gia tac dung ciia bai tap dudng sinh Nguyen van HuoTig tren goc tir do a d6i tupng beo phi. Chinh vi vay, chiing toi tien hanh de tai nay nham mue tieu:
"Ddnh gid tdc dung chong oxy hoa cua phucmg phdp luyen tap duang sinh Nguyen Vdn Huang tren benh nhdn beo.phi"
II. DOI TLTgnVG VA PHLTONG PHAP NGHIEN ClTU
2.1. Do! tirtfng nghien cihi G6m 60 doi tupng beo phi, tap luyen tai khoa Cham ciiu duang sinh - Benh vien Y Hpc Co Truyen Trung LTong.
2.1.1. Tieu chudn chpn doi tup'ng:
- NU giai, do tuoi tir 40 - 60 tuoi, ty nguyen tham gia nghien ciiu
- Dli tieu chuan chan doan beo phi theo TCYTTG co BMI > 25
2.1.2. Tieu chudn logi trir - Cac doi tupng co dii tieu chuan tren nhung dang mac cac benh nhiem trung, nhiem virus, cac benh cap tinh khac suy gan, suy than, ung thu,...
- Khong tuan thu quy trinh nghien ciiu
2.2. Phirong phap nghien curu 2.2.1 Thiet ke nghien cuu:
* Phuong phap nghien ciiu: nghien ciiu can thiep, so sanh truac va sau dieu tri, so sanh voi nhdm chiing.
* Co mau nghien ciiu: Gom 60 doi tuong dupc chia thanh 2 nhom
+ Nhom Nghien ciiu (NC): 30 doi tuong dupc luyen tap theo phuang
Nghiin ciiu YDHCT Viet Nam phap Duong sinh cua Nguyin Van Hucmg [4]
- Nhom Chiing (C): 30 doi Uiong duoc luyen tap theo phuong phap Hubbard [5]
Ca hai nhom d6u dugc uong thuoc b6 trg theo phac do nen
Betex (Bl, B6, B12) x 2vien/ngay uflng chia sang chiSu sau an X 15 ngay
Omega-3 (lOOOmg) x 2vien/ngay uong chia sang chieu sau an xl5 ngay
2.2.2. Ngi dung luyen tap
* Nhom nghien cuu
- Tap luyen theo bdi tap DS cua BS Nguyen Vdn Huang
Gom cac phiin: Luyen thu gian- Luyen tho- Luyen a tu the dgng (tu xoa bop, tap van dgng
* Nhom chung
+ Tap luyen theo phucmg phdp Hurbbard
Bang 3.1; Ket qua thay doi nong do enzym chong oxy hoa SODtp
& cac thoi diem nghien cuu.
G6m cac phSn: chixy bo - Tdm hai ndnggidn doifn (xong hai ndng).
- Thai gian tap tir 15h den 16h30 phut hang ngay, lieu trinh hong vong 15 ngay, tai Benh vien Y hpc c6 truyen Tnmg LTong.
2.2.3. Chi so nghien ciru
* Nflng do cac enzym chflng oxy hoa: SOD, TAS, GPx va nflng do chit oxy hoa MDA tai thoi diSm Do, D15 2.3. Bia diim vii thoi gian nghiSn cihi;
Thoi gian nghien cuu: Tit thang 5 den thang 10 nam 2012.
Tai + Benh vien Y hoc c6 truyen trung uong.
+ Labo xet nghiem trung tam - Benh vien Dai hoc Y Ha Ngi.
3.4. Ket qua nghien cihi
* Slf thay doi nong do cdc enzym chong oxy hoa sau 15 ngdy luyen tgp.
^ ~ ~ - ^ ^ ^ Chi so SOD.p Thoi diem danh g i a ^ - ^ ^
Do D,5 p(Di5-Do)
PNC-C
Nong do enzym SOD,p (U/ml) (X±SD) NhomNC
(n=30) 172 ± 27,00 184 ±33,00
< 0,001
NhomC (n= 30) 208 ± 35,00 211 ±33,00
<0,05
>0,05
Journal of research in viet nam traditional medicine and pharmacy No 40 - 2014 Nhan xet: - Sau 15 ngay luyen tap, nong dp enzym ch6ng oxy hoa SODtp tang co y nghia p < 0,001 (nhom nghien ciiu) va p < 0,05 (nhom chiing) va sir khac biet giiia hai nhom khong co y nghia th6ng ke p>0,05.
Bang 3.2: Ket qua thay doi nong dp enzym chong oxy hoa TAS of cac thoi diem nghien cmi cua hai nhom.
^ " ^ ^ . ^ ^ Chi so TAS Thoi dilm danh gS^^.^^^
Do D,5 p(D,5-Do)
PNC-C
Ham Ivong TB enzym TAS (mmoI/1) (X±SD)
NhomNC (n=30) 1,78 ±0,11
1,95 ±0,38
< 0,001
NhomC (n= 30) 1,75 ±0,09 1,89 ±0,14
< 0,001
>0,05
Nhan xet: - Sau 15 ngay luyen tap, nong dp enzym chong oxy hoa TAS tang co y nghia p < 0,001 6 ca hai nhom va su khac biet giua hai nhom kh6ng co y nghia thong ke vai p> 0,05.
Bang 3.3: Ket qua thay doi nong dp enzym chong oxy hoa GPX|p cua 2 nhom is cac thoi diem nghien cihi
^ " ~ ^ ^ ^ , ^ ^ Chi so GPxip Thoi diem danh gia"^^.,,^^^
Do D,5 P(D,5-D„)
PNC-C
Nong do enzym GPx,p(U/gHb) (X±SD)
NhomNC (n=30) 8449 ± 8744 9023 ± 1749
< 0,001
NhomC (n= 30) 7872 ±9178 9988 ±11011
< 0,001
>0,05
Nhan xet; - Sau 15 ngay luyen tap, nong dp enzym chong oxy hoa GPx,p tang co y nghia p < 0,001 a ca hai nhom va khong c6 su khac biet giira nhom voi p>0,05
Bang 3.4: Ket qua thay doi nong do enzym chong oxy hoa GPxhc trung binh cua hai nhom o cac thai diem nghien cihi
^ ~ ~ ~ ^ ^ ^ ^ Chi so GPxkc Thoi diem danh g i S ^ ^ ^ ^
Do D,5 P(D,,-D„)
PNC-C
Nong do enzym GPxhc(U/gHb) (X±SD)
NhomNC (n=30) 69.24 ± 16.74 90.20 ±17.54
<0,01
NhomC (n=30) 59.96 ±20.51 72,86 ±27.19
<0,01
>0.05
Nghien cAu YDHCT Viet Nam
Nhan xet: - Sau 15 ngay luyen tap, nong dp enzym chong oxy h6a GPxhc tang co y nghTa p < 0,01 o ca hai nhom va khong co su khac biet giiia nh6m NC va C voi p>0,05
* Sur thay doi nong do goc tir do sau 15 ngay luyen tap.
Bang 3.5: Ket qua thay doi nong do MDA cua hai nhom o cac thM diem nghien cihi
^ ^ ~ ^ „ ^ ^ Chi s6 MDA Thcci diem danh gia-.^^^^
Do D,5 P(D,5-D„)
PNC-C
Nong do MDA (mmol/l) (X±SD) NhomNC
(n=30) 0,86 ± 0,25 0,75 ± 0,34
>0,05
NhomC (n= 30) 0,59 ± 0,39 0,65 ± 0,36
>0,05
>0,05 Nhan xet: - Sau 15 ngay luyen tap,
nong do goc tu do MDA thay dfli chua CO y nghia thflng ke p > 0,05 o ca hai nhom va khflng co su khac biet ffiiia 2 nhom vai p>0,05
IV. BAN LUAN
* Ve su- thay doi nong do cac enzym chong oxy hoa va goc tur do sau 15 ngay luyen tap duong sinh Nguyen Van Hu-irng.
4.L Anh hirong ciia luyen tap duong sinh len hoat do enzym SOD.
SOD (Superoxid dismutase) la chat chflng oxy hoa co ban chSt enzym, no co trong hflng ciu (SODhJ hay trong huyet tuong, dich mfl, dich tiiy s6ng,...(S0D,p) [3]. Ket qua nghien cim o Bang 3.1 cho thay: Sau 15 ngay luyen tap, nflng do enzym SOD,p tang cfl y nghia p < 0,001 (nhom nghien
cuu) va p < 0,05 (nhom chiing) va su khac biet giira hai nhom khflng co y nghia thflng ke. KSt qua nay cho thiy ham lugng SOD tang len trong qua trinh luyen tap dflng nghia voi qua trinh khii goc ty do nhieu ban.
4.2. Anh hirorng cua luyen tap len hoat dg enzym GPx.
Glutathiol peroxidase (GPJ co o hau het cac mfl, chiem vi tri quan trgng thir hai trong he thong oxy hoa cua te bao, sau SOD [3]. Kflt qua 6 Bang 3.3, 3.4 cho thay, sau thoi gian luyen tap boat do trung binh cua GPxhc, GPxip o ca hai nhom dflu tang len ro ret. Su khac biet truac va sau luyen tap a hai nhom CO y nghia vai p<0,001.
4.3. Anh hu-o^ng ciia luy^n tap len nong do enzym TAS.
Journal of research in viet nam traditional medicine and pharmacy
TAS la trang thai ch6ng oxy hoa toan phan ciia co the bao g6m nhieu he thong phong thii d^ giiip co the ch6ng lai nhiing anh huang co hai ciia g6c tu do va hien tuong peroxi hoa cua co th^
[3]. K8t qua a bang 3.2. cho thay n6ng dp enzyme TAS dupc tang len ro ret sau luyen tap duong sinh hay Hubbard vai p<0,001. So sanh giiia hai nhom a cac thai diem nghien ciiu kh6ng co sir khac biet voi p > 0,05. K6t qua nay chimg minh trang thai chong oxy hoa toan phlin (TAS) diu co anh huong tich cue tii phuang phap luyen tap dudng sinh va phuong phap Hubbard
4.4. Anh hirmig cua luyen tap len MDA
MDA trong huyet tuang phan anh gian tiep boat dp ciia goc tu do th6ng qua qua trinh peroxi boa lipid [3]. Ket qua nghien ciiu o bang 3.5 cho thay ham lupng g6c tu do gian tiep MDA ciia nhom luyen tap duong sinh Nguyen Van Huofng c6 giam nhe nhung chira co y nghia th6ng ke voi p
> 0,05.
Nhu vay, sau luyen tap bang phuang phap duong sinh (nhom nghien ciiu) va phuang phap Hurbbard (nhom chiing) deu lam tang nong do enzyme ch6ng oxy hoa nhu SOD, GPx, TAS va dac biet phuong phap luyen tap duong sinh Nguyin Van Huong da co diu hieu lam giam
n6ng dp goc tu do MDA trong huylt tuong (tuy chua co y nghia thong ke).
Su cai thien nay buoc dau cho th4y khong CO sir khac biet giiia hai nhom.
Phuong phap Hubbard la phuong phap da duoc chiing minh qua nhieu cong trinh nghien ciiu la co tac dung dieu chinh g6c tu do hay thai dpc t6t qua con duong m6 hoi [8], [9]. Trong khi do phuang phap tap duong sinh Nguyen Van Huang [4] la phuang phap luyen tap tong hop, trong do phuang phap tho 4 thi giiip nang va day CO hoanh xuong sau lam long nguc gian t6i da lam tang dung tich s6ng, tang chiic nang trao doi khi a phoi giiip duang khi duoc vao co the va than khi cung cac chat dpc theo duang ho hap cung dupc thai ra den miic toi da; tho 4 thi tao dieu kien tang cuang mau ve tim tot va tong mau vao long mach tot, tang luu luong tuan hoa giiip mau di nu6i duong toan than, qua trinh trao doi chat 6 mo va cac ca quan duoc t6t, ket hap vai thu gian, tho bon thi va luyen dpng giiip tang cuong luu thong khi huyet den nhiing noi xa nhat trong CO the, luu luong mau den gan, than tot hon giiip cho viec bai tiet cac chat dpc hai qua nuoc tieu; tang cuong chiic nang thai dpc ciia gan, trao d6i chat a mo, o da duoc tang cuang lam tang kha nang bai tiet chat dpc hai qua duong mo hoi Nhu vay phuang phap
Nghien cdu YDHCT Viet Nam So 40' 2014
luyen tap Nguyen Van Huang la phuang phap tong hap bao gom luyen thu gian, luyen tha bon thi, luyen dpng da dat dupc hieu qua trong viec dao thai cac chat oxy hoa hay chat dpc hai ra khoi co the o benh nhan beo phi qua nhieu con duong.
V. KET LUAN
Luyen tap duong sinh theo phuang phap Nguyen Van Huong buoc dau co
tac dung lam tang cac chat chong oxy hoa tren benh nhan beo phi the hien:
N6ng dp enzym chong oxy hoa SOD,p, TAS, GPx,p, GPXh dau tang sau 15 ngay luyen tap vai (p< 0,01, p<0,001) a ca nhom tap Duong sinh Nguyin Van Huong va nhom tap Hubbard, sir khac biet giiia hai nhom khong co y nghia th6ng ke vai p>0,05.
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G.( 2006) Obesity: An independent risk factor systemic oxidative stress. Park.J.
Pharm.Sci. 19,62-69
2. Monteiro, R.; Azevedo, I.
(2010), Chronic Imflammation in obesity an the metabolic syndrome.
Mediators. Imflamm, 2010. 289.645.
3. Roberta J.W., Timothy J.P., (1995), 'Tree radicals", clinical biochemistry - metabolic and clinical aspects, pp 165-111
4. Nguyen Van Hirong (1995), Phuang phdp duang sinh, Nxb Y hpc Thanh ph6 H6 Chi Minh, tr.70-90.
5. Hubbard L. (1979) "Purificasion rundown replaces the sweat program", HCO Bulletin.
6. Nguyen Thi Van Anh (2000), Nghien cim tdc dung bdi tap duang sinh ciia Bdc sT Nguyen Vdn Huong tren benh nhdn co hoi chung thieu ndng tudn hoan
nao man tinh, Luan an Thac sy Y hoc, Truang Dai hpc Y Ha Npi.
7. Vo Lull Hoa (2010), Bdnh gid tdc dung bdi tap duang sinh cua Nguyen Vdn Huang tren doi tuang cong nhdn phai nhiem xang ddu co hoi chimg nhiem dpc bezene nghe nghiep, Luan van thac sy y hpc, Trudng dai hpc y Ha Npi.
8. Tru-ffng Thi Xuan Hoa (2012),
"Ddnh gid tdc dung bdi tap duang sinh cua Nguyen Vdn Hudng tren dSi tupng cuu chiin binh phai nhiim Dioxin", Luan van chuyen khoa II.
9. Nguyin Van Tu-ong (2011), Ddnh gid hieu qud dieu chinh gdc tu do cita phuang phdp Hubbard trong thanh Ipc chdt dpc ra khoi ca thi, 6k tai nghien ciiu khoa hpc, tr. 8-13.
