• Nghien cu-u - Ky thuat
Hai dammaran saponin phan lap tir loai Gynostemma burmanicum King ex Chakrav.
Than Thj Kieu My", Phjm Thanh Ky, Pham Tiian Anh
Bo man Dupe tieu. Trvcmg Bai hoc Dime Ha NQI 'E-mail: thankieurnyl^gmail.com Summaryeth^!r^Me7bi^Z^^'^'^r!""'^''^''''^"''"''="-''^''''"'''''P^''«^"-^'r'l'>''-hexan
Keywords. Mian dian Jaoguian, Gynostemma burmanicum. dammaran saponin.
Precisa, tu say Memmert, may c j l quay Buchi Rotavapor R-200, bin mong silica gel trang sin G F ^ (Merck).
- Silica gel pha ttiuwng (Merck), c& liat 0,063- 0,200 mm, silica gei pha 6io RP18 (Merek) sephadex LH20 (Merck).
- Bo pho kh6i tr§n may Agilent 1100 LC-IV1SD- Trap-SL tai Vien Ho^ hoc - Vien Han lam Khoa hoc vi cong nghJ M ^ Nam.
- Bo ph6 cOng h u * i g tir hat nhin tren m i y Bruker AM-500 tai Vi^n Hoa hoc - Vi?n Han l i m Khoa hpc va cong ngh0 Vi$t Nam.
- C i c h b a c h ^ , thuoc thi> dat tiSu chuin phan tich.
Phuwng p h i p nghien cCinj
Chiet xu4t cao toan phJn tii duoc lieu bang phuOTig phap chiit h6i li/u v i i dung moi l i ethanol 70/4, sau do chiSt vin cic dung moi cd dp phin cjpc tang dJn gSm n-hexan, ethyl acetat, n-butanol Binh tinh cac phan doan thu du^c bSng phin ung hoa hpc va sac ky top mong theo tai lipu «'.
^ Phin lap saponin tir c i n phin doan n- butanol bang sac ky cpt vdri chit nhol cpt la silica gel pha thyong (co hat 0,063-0,200 mm), silica gel pha dao RP18 v i sephadex LH20. Kiim tra dp tinh khiSt cua chSt phin IJp du'pc b4ng sSc k« Idp mong. ' ^
Nhan dgng chit phin l i p dupe bing phuonq phip phin Bch do liju ph6, bao gSm ph6 ESI-MS 'H- NMR, " C - NMR, BEPT, HMBC, HSQC
€ a t van de
Giioc4lam(GCL)Gynostemmapen(ap/,y//um (Thunb.) Makino thu h i i » Viet Nam da dupe Chung minh mOt s6 t i c dgng dang chu i nhu ha cholesterol w, ha dupng huylt, tang cuOng d i p ling miin djch H, I>C c h i kh6i u i»i. Nhom chit hi>u CO dupe quan t i m nghiin cuu trong c i c loii thuoc chi Gynostemma Blume l i saponin va flavonoid. Qua khao sat, loii Gynostemma burmanicum King ex Chakrav. (giio c6 lam Miin B«n), thu h i i tai B i c Kan da dupe ngu*i dan d|a phuwng si> dgng nhung chua cd nghiSn ci>u nao vi loii n i y * Vi#t Nam. B i i b i o niy cdng bo ket qua phin l i p v i nhin dang hai saponin ti> loai Gynostemma burmanicum King ex Chakrav thu h i i tai B i c Kan.
Nguyen l i f u va p h l K m g ph^p Nguyen l i | u
Mau nghien cuu thu hii tai huyjn Chp B6n tinh Bao Kan v i o thing 5 nam 2015, da dupe thani dinh ten khoa hpc l i Gynostemma burmanxum King ex Chakrav. (giio co lam Miin Bien) tieu bin luu tro tai BO man Thg-c vjt - Tnrong Bai hpc Dupe H i N«i (ky hipu 24/2015). Bupe iiju sau khi thu hil phoi 6. noi thoing mat, sly p nhi#t dd 6 0 ^ v i b i o quin trong tOi nilon kin 6 noi khd rao ddng l i m nguyen li§u chilt xult.
H6a chat VS thiet bj nghien c m i - C i n ky thuat Saitonous, cin phin ttch TAP CHi Dir<?C HQC - 9/2016 ( S 6 4«5 NAM 56)
• Nghien ciJu - Ky thuat
Ket qua va ban luan Ket qua ve c h i l t xuat
Chilt hoi luu 3 kg giio co lam Mien Bien khd vd'i 12 L ethanol 70% a 80°C, chilt 3 Ian, m i i l i n 60 phut. Gpp djch chiet 3 lin. d t thu hoi ethanol dual ap suit giim thu dupe djch chiet nupc, de lang qua dSm, va ipc lay djch Ipc. Tilp tyc co djch Ipe o 100"C de thu dupe dich dam die. TTiem nupc c i t
vao dich dam die, l i e cho phan t i n d I u r6i chill l i n lupt vd'i n-hexan, ethyl acetat, n-buthanol. M5i dung moi lie 3 Ian, gOp cac djch chilt thu dupe, cat thu h i i dung moi dudi ap suit g i i m thu duvc eac can H (13 g), E (84 g), B (180 g) v i phin dich nud'c c6 d i n can N (36 g).
^ Bjnh tinh saponin trong c i c d n H, E, B va N bang phin ling hda hpe oho k i t qua saponin c6 trong d n B va N.
PtiiaiCmg Hien tiling
Hien tirong tao Ixpf OflttxittjensaulSphtit Hien tiring ptd huyet Hong cau t)i piia v& tren phiert ki Phan i)ng Liebemann-Burchard V6ngti'mS6gii>a2lffpdiatteng Phan img Rosenthaler Mau xanh tfm
Binh tinh d n B va N bing d e kj lop mdng v6i h i dung mdi CHjCI^- MeOH - H p (4 : 1 : 0,1), hipn v l t bang phun vanillin/ H^SO, 10%/ ethanol.
say 11()»C trang 10 philt. K i t qua cho thIy cin B cho 6 vet ro, d n N cho 4 v l t rd.
P e a t Steit
Total Height 22S9 96 Rf
0.564 0.623 0.685
0 764 0.829 0.874 ToiaAeaWax.
J 5 a _
1357 3 60.06
jg1.9 12.92
0592
283614 Hmti ^: sic ky do cin B
IM
9J8
T ^ CHf DirgrC Hpc - 9/2016 (S6 485 NAM 56)
Nghien cCeu - Ky thuat
T i K k 8
Peak Stan0 8 1 6
Rf Max.
933.6 J»L
61.03
Total HeIsM 1S29 57 ToiaAea 115967 6U
End
0-872 1.7 3604.6
57.22
31.0B
1284.51 l l . o T Hlnh 2: SicKet qua phdn l^p
C i n khoing 10 g d n B hda tan voi lupng t i i Ihilu methanol r l i h i p phu vao lupng vi>a du silica gel cp hat 63-200 pm. Khai trien cpt d o ky silica gel CCT hat 63-200 pm, rira giii bing hp dung moi CHjClj - MeOH - HjO cd do phan cue tang d i n (7:1:0.1; 5:1:0.1: 3:1:0.1; 1:1:0.1), 1 L mSi ho, thu dupe 4 phan doan kii hipu thu tv la B l B2 B3
B4. . . .
Cit thu h i i dung mdi phin doan B4 toi d n thu dupe khoing 4,40 g c i n . Tilp tge phin tach d n B4 vdi c i t sephadex LH20, n>a giii bing ethanol 70%. Hung djch n>a giai vao eac ong nghipm cd dinh s i . Kilm tra c i c Ing bing TLC, hp dung mdi khai tnen CH,CI,-MeOH- H,0 (4:1:0,1), thule thu vanillin«^SO, 10%/ ethanol. Gpp cic Ing cd thinh phin tuong tg. nhau thinh 3 phan doan B4 1 B4.2,B4.3.
C I tpi d n phin doan B4.3 thu dupc 0 70 g d n , tiep tge then khai s i c ky cpt vol ehat'nhli d t l i silica gei pha d i p RP18, n>a giii bing he dung m l i MeOH - H,0 (70:30). Hu'ng dich n>a giii T*P CHl DirtJC HQC - 9/2016 ( S 6 485 NAM 56)
•kfdScinN
vao cac I n g nghiem cd dinh so. Kiem tra cic phan doan bing U C , gpp cac ong cd cae v l t tupng Hr.
Tif Ing s i 103- 130 thIy xult hi#n 1 v l t m i u tim d i m , cd tdi d n , ket tnh lai nhilu l i n thu dm?c 141 mg chit k i t tinh m i u tring, ky hiSu l i GB. Bu-02.
TO' Ing s i 142-170 thIy xuit hi|n 1 v l t m i u tim dam, CO ton d n , k i t tinh Ipi nhieu l l n thu dupc 32 mg chat kit tinh m i u trang nga ky hieu l i GB. Bu-01.
N h i n dang eac hpp chat Hop chat GB.Bu.02
^ Hpp chat GB.Bu-02 thu dupc dupi dang b|t ket tinh mau trang. P h i ESI-MS cho pie [M+NaJ- 1101,5 m/z, tCf dd eho thIy M = 1078, tuwng O'ng vdi cong thue phin tl> CggHjpO
Pho <H-NMR eua GB.Bu-02 xult hipn ehu y l u cae tin hiiu thupc 2 vung, vung tnrang cao l i c i c tin hipu cua phin aglycon, vung tnrang trung binh la eac tin hipu eOa c i e phan ti> duirng. Xem cac tin hieu eua khung aglycon thiy xuat hi|n
• Nghien cu'u - Ky thuat
8 nhdm methyl dud'i dang singlet tai 5„ 0,88 (3H, H-29); 0,94 brs (6H, H-19 va H-30); 1,03 (3H, H-18); 1,09(3H, H-28); 1,38 (3H, H-21); 1,65 (3H, H- 27) va 1.71 (3H, H-26). Tin hieu 1 proton olefin duoi dang triplet tai S„ 5,15 (1H, t. J = 7,0) gpi y su cd mat cda 1 lien k i t d l i trong khung d u trOc GB.Bu-02. Tin hiiu cua 4 proton anomer tai 5„
4,32(1H,d,J = 7,0Hz);4,45(1H,d.J = 7,5 Hz);
4,59 (1H, d, J = 8,0 Hz) va 4,70 (1H, d, J = 8,0 Hz) chd thay k h i nang t i n tai 4 dud'ng trong d u tnic cua GB.Bu-02.
TO p h i "C-NMR, DEFT 90 vi DEPT 135 cd the nhin thay tin hieu cua 53 nguyen tt> carbon, trong dd cd 8 nhdm CH3-, 13 nhdm CH^<, 26 nhom - C H < v i 6 c a r b o n b i c 4 .
Phin tieh cic tin hipu tren p h i HMBC cho thay tucng tic giua proton olefin H-24 (8„ 5,15) vdi C-26 (Sj 25,9)/C-27 (6^ 18,0); tupng t i e giija proton H-26 (5„ 1,71) va H-27 (5„ 1,65) vpl C-24 (5j 126,1) va C-25 (5^ 132,2) khang djnh su cd mat cOa nil ddl tpi vj trf C-24. Cac tuong t i c xa giiya prptpn H-19 (S„ 0,94) vdi cartjdn 0-9 (6^ 51,1) va C-10 (5^ 37,9), proton H-21 (5„ 1,38) vpi carbon C-17 (Sj 52,8) v i C-22 (5^ 36,7) cung khang «nh vi tri nhdm methyl d eic vi tri n i y
K i t hpp diJ Iliu pho HSQC vi HMBC khang dinh vi trf cOa nhdm methyl d vj tri 0-19 v i C-30 CO pie tin hieu proton d n g huwng tai vj tri 6„ 0.94,
Tin hieu tuong tac HMBC eua khung aglycon tai C-3 (6^ 91,3) vdi proton H-1' cDa phin ti>
d u l n g p-D-glucosetai6„ 4,45 (1H, d, J = 7,5 Hz);
tupng t i c eua C-20 (6^ 84,98) vol proton H- r tai 6„ 4,59 (1H, d, J = 8,0 Hz cda phin tl> P-B- glucose thu hai khang djnh vi tri gan eac dupng tai 0 3 va C-20. Cac dud'ng eon lai cung dupe nhin thay tuong t i c eua C-Z (6^ 81,02) cda phin tir du^mg 3-B- glucdse vd'i H-1" tai S„ 4,70 (1H, d, J
= 8.0 Hz) cua p-D- glucose thu' hai; v i turang Be cua C-6'" (65 70,05) cda du^mg p-D- glucose vol H- 1"" tai 5„ 4,32 (1H. d, J = 7,0 Hz) eua dupng a-l_-arabindpyranosyl. Cae tin hi0u tuong tac tren p h i HMBC cho thIy GB.Bu-02 la mpt saponin triterpen cd phin aglycon l i khung dammaran v i CO 4 phan tie dupng, trong do tai vi tri C-3 cd 2 phin tu. dud'ng p- D- glucose, vl tri C-20 cd 1 phan tu' dud'ng p-B-glucose v i 1 phin tu duerng a-L- arabinopyranosyl.
So sinh so Ilpu pho ghi dupe cfla GB.Bu-02 vol s i lipu pho trong t i i lipu tham khio H (bSng 1) gpi y c i u tnlc GB.Bu-02 nhu hinh 3.
Hinh 3; Cau triic GB Bu-02 Bang1:5iil/pup>io NMR cua hgp chit GB.Bu-02
c
1 2
^C 39,6 26,7
4."
40,2 27,2
^"
1,02m/1,76m 1,34 m/1,91m
HMBC B g 10 11 12 13 14 15
40,5 50,6 37,3 31,1 70,6 49,8 51,8 31,1
40,9 51,1 37,9 31,5 71,1 49.5 52,4 30,8
1,47 m
1,06 nV 1,81m 3.50 m 1,76 m
1,28 nV 1,78 m IB 19 19
30 18
TAP CHf DtrqC H p c - 9/2016 ( S 6 485 NAM 56)
• Nghien ciJu - Ky thuat
?y '/.g l.74miz,lBin 9lueopyranosyl]-20-0-[p-B-aiucopyranosyl(1^6)- 52.1 52.a 2,3ld(l(a.5-l0,5) 21 a - l - - a r a b i n o p y r a n o s y i ] - 3 j e , 1 2 ; 8 , 2 0 ; S - 16.4 16,3 ijCKs trihydroxydammar-24-en. Hop c h i t n i y d i d u p c KJ KJ 0.94 lis l i o ^ " * * Panax ginseng ( n h i n sam), Panax e3.s 84.9 - „ 2 1 r rmtoginseng (tam thit).
a ' 22.3 1.38 s ' 1 7 2 — Nhan dang hifp chat GB.Bii-01 W 36,7 1.54ir<181rii 21 * P * ^ ' G B . B u - 0 1 t h u d u p c d u d ' i d a n g 23.6 23,8 206mate * ' ' ^ ' ' " ' ' "^" " ^ " ^ " 9 ^ - P * ' * ^ S / - M S c h o p i c
„ „ , „ ; -^^^^^ ' " ' ung VPI clng thuc phan ti>C„Hn,.
| J 1 , J 132,2 - T - , , _ . . . , , 47 so 17
— _ T u d u lieu c i c pho 'H-/V1MR, "C-AIMR, DEPT ZZ ^ ' !^^!i ! ? — 90, DEPT « 5 cda GB.Bu-01 nhpn thay cd k h i
— ' "'° ! * i ! 24 nhieu diem tuong t u n h u cae du' l i i u p h i cSa hpp a s 28,4 1,119 s 4,5 c h i t GB.Bu-02. Cu t h l la:
" • ° ' ° . ' °.°»= 3 _ _ P h i 'H-NMR eua GB.Bu-01 cung x u i t h i i n c i c '76 1/.4 1194s 13 " n hipu t h u l c 2 vung, vung tnjiing cao l i eac tin h i i u cua phan aglycon, vung tnrdng trung binh 105,3 105,3 4,45 d (7,5) I '^ '^^ " " ^Wu cOa c i c p h i n ti> dudng. Xem eac 83.7 81,1 i ^ y "h h*d d a khung aglycon thiy xult hien 8 nhdm 784 774 ts^ ™ * ^ ''"*'' '^"^ = ' " 9 1 * 1 Praton olefin dupi 721 71; ^ f ? ?^"9 " P « >«! 5 5.15 (1H, t, J = 7,0), proton niy , . ; " . =2^5 tuong t i c vdi vol C-26 tpi 5„ 25,9 v i vdi 0-27 tpi a
" ' ' " • ' IHiD 18.0 trdn p h i HMBC B i l u niy cho thIy kha nang ' ' ' ' ^' " ' " " M m hpp chit GB.Bu-01 cd cung c i u tnjc phin aglycon '_ la khung dammaran vdi GB.Bu-02.
106,2 104.4 4,70(1(8,0) 2 Juy nhidn, tiF vi#e phin tieh eac p h i 'H-NMR '"S 76,6 3.42 m Ph" "C-NMR ciia GB.Bu-01 thIy thilu 6 tin hi$u 76,4 78,5 zsiii '"i ' ^ * ° " " ^ 1° "n hiSu proton so vdi dii li#u 72,0 71,4 J^ P ™ "="= GB.Bu-02. N h u v i y cd kha nang hpp 782 777 J^ chit GB.Bu^ll cd d u tnjc tuong tg. nhu hpp chit
^ - n ! ™ ! _ P h i 'H-NMR xuit hiln tin hipu cua 3 proton
„ „ . „ . . a"dniertai8„4,33(1H,d,J = 7,0Hz);4,32(1H d
- s H f ' - ^ 2 ^ thi 'fh"''-"' "•?' ""• '••'-'•'> "^) ^h«"9 * h
' " « 3,14 m t h i m kha nang ton tpi 3 d u ^ n g trong d u trde cCia 78,6 78,4 336^^ GB.Bu-01.
S8.8 71,5
M7m P h i n tfch eac tin h i j u t r i n p h i HMBC cho thay 3^4 m d i e tupng t i e cda khung aglycon tai C-3 (6 90 6) 3,37iTi».03a(lO) r— ! " ' P™*"" " " 1 ' ""^ P h ^ " 1 * ' ' " i n s P-D-glucose tai
«H '1.33 (1H, d, J = 7,0 Hz); tai 6- 84,9 ( C-20) v l i 432dl70) F — f . T , ^ " : ' " ' ^ ' ^ " ' ' ' ^ ' < ^ " ' ' ' " ' = 8 ' ° H ^ ) ' = " = P h a n
^ : ^ ™ ) 5 tu p-[>glucose thii hai; tin hi|u lupng t i c tai C-6"
MiiE (5j 70,1) eua dui'ng p-l>.glueose vdi H- V" tai 5
—^^ ' ' . 3 2 ( 1 H , d , J = 7,5Hz)euadu6ngarablnose "
— ^ ^ ,'^=«^ tin h i i u tuong t i e tren p h i HMBC cho
^.'im'J.'em thay GB.Bu-01 l i mpt saponin triterpen e l p h l n '«ctimgCD,OD,'ih&l!5MHz,'ilo6S00UHr, aglycon la khung dammaran v i cd 3 p h i n ti>
'sd mi pti6 Its cang b6 tmng 1^1 li4u^ •'^^hS. trong dd 2 phin tu. dudng p-D-glucose 1 can cu v i o s i Iliu phi ghi dupc va d l i chilu P^"^" » " " ^ ^ S °-l-arabinopyranpse.
vdi t i i liiu tham khio II, hpp chit GB Bu-02 dupc *• * , ? " ' ' u . ' * " P^° * ' dupe cua GB.Bu-01 xic dlnh l i : 3 - a , p - D g l u c o p ^ n e s y l ( 1 . 2 , r D - gpl f e l f t r S c G r B l ^ ; S S 4 ' ' ° ' ' * ' " ' ^ ' T^T CHi DirtJC HQC - 9/2016 ( S 6 485 N A M 56)
• Nghien cipu - Ky thuat
Hlnh 4: Cau (r Bang 2: S6 lieu pho NMR cua GB.Bu-01
c
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 28 30
S*
39,6 26,7 89.3 40,1 56,8 18,9 35,6 40,5 50,6 37,3 31,1 70,6 49,8 51.8 31,1 27,1 52,1 16,4 16,7 63,8 22,7 36,6 23,6 126,1 131,3 26,2 18,3 28.5 17,0 17,8
S^
40,2 27,2 90,6 40,3 57,6 19,2 35,8 40,9 51,1 37,9 31,5 71.1 49,7 52,4 30,8 27,2 52,9 16,3 16,7 84,9 22,4 36,7 23,8 126,1 132.2 25,9 18,0 28,4 16,8 17,4
mutt (J in Hz]
1.02 m/1,74 m 1,74 m/1,89 m 3,17 m 0,81 d (11) 1,49 m/1,58 m 1,31 m/1,57 m 1,45 m
1.03 s/1,58 m 3.75 m 1,76 m
1,31m/1.81m 1,74 m/1,96 m 2,31 dd (8,5-10,5)
1,03 s 0,94 brs
1,38 s 1,56 m/1,81m 2,06 m/2,17m 5,151 (7,0)
1.71s 1,65 s 1,07 s 0,90 s 0,94 bfs
HMBC
r, 28.29
IS, 28, 29 18
19
30 18
21
17,1"
17 26,27
27 26
5 3-Glc
1' 2' 3' 4'
105,3 83,7 78,4 72,1
106,7 77,5 78,3 71,7
4,33 d f7,0) 3,33 m 3,66 m 3,28 m
3
5' 6'
78,2 63,1
77,7 Q2.B
3,38 m 3,31m/3,83 m 2[>^lc
1"
2"
3"
4"
5"
6"
98,4 75,2 78,6 71,2 76,9 69,5
98,1 75,6 78,6 71,4 76,6 70.1
4,59 d (8,0) 2- 3,14 m 3,35 m 3,27 m 3,24 m 3,75 m/4,03d (11,5) 1'"
20-Glc-Ara 1'"
2'"
3'"
4'"
5"' 104,7 72,4 74,3 68,8 65,7
105,5 74,8 75,2 71,1 66.7
4,32(1(7,5) 20 3,23 m
3,23 m 3,36 m 3,18 m/3,87 m ' do trong CDfiD, '•3od125 MHz. 'dodSOO MHz,
' s6 ii$u ph6 di cong b6 trong t^i li$u "<
Can ci> v^o s6 liSu pho ghi du-g^ vk d6i chieu vdi tai ligu tham IthSo PI, hop chat GB. Bu-01 difp'c xac djnh Id: 3-O-)3-Dglucopyranosyl-20-O-
[p-D-glucopyranosyl(1-^6)-a-L-arabinopyranosyl]- 3p,12p,20p-trihydro)q«Jammar-24-en. Hpp ch^t nSy da dugc phSn l^p ti> Panax ginseng (nhan sam).
Ket luan
Da chilt xuat saponin tir \oia Gynostemma bumianicum King et Chakrav. thu h^i d Bac Kgn.
K i t qua djnh tfnh cho thay phSn do^n chilt bing n-butanol chiia nhilu saponin han c5.
Bing phyo-ng ph^p sac k^ c$t da phan lap du'p'c hai dammaran saponin, k^ hi0u la GB.Bu-02 va GB.Bu-01. Can ci> vao cdc dO liSu pho MS, p h i 'H- NMR, "C- NMR, DEPT, HSQC, HMBC vS so sdnh vd'i dO li^u pho & tdi lj$u tham khao, da nh§n dgng du-p-c GB.Bu-02 Id: 3-O-p- Dglucopyranosyl(1-»2)-p-D-glucopyranosyl]- 2 0 - O - i p - D - g l u c o p y r a n o s y l { 1 - » 6 ) - a - L - arablnopyranosyl]-3p, 12p,20p-trihydnDxydammar- 24-en hay cdn gpi Id ginsenosid Rb2; da nh|n dgng T ^ CHi DVQC HQC - 9/2016 {S6 485 NAM 56)
• Nghien ciru - Ky thuat
dup'C GB.Bu^)1 Id: 3-O^-t>fllucopyranosyf-20-O- [p-D-glucopyranosyl(1-.6)-ct-L-arabinopyranQsyl]- 3^, 12p,20p-bihydroxydammar-24-en.
Day la cong bo dIu tien ve dammaran saponin phdn Idp Kf loar Gynostemma bunnanicum King ex Chakrav. (giao co lam Miin Dien) thu hdi a Viet Nam.
Tai lieu tham khao 1. Pham Thanh K^, Vu Di>c Canh, Phgm Thanh Hucng, Nguyen Kim Phirong (2007), "Nghign cmj tac dgng ha diolesterol mau cua duoc ttSu giao c6 lam - Gynostemma pentaphyllum (Thunb.) Makino," Tap chi Du^hQC. so 373, thang 5/2007 tr 9-10
2. Phgm Thanh Ky, Phan Thj Phi Phi, P. T. T. Anh (2007), "Nghien ci>u tec dyng tang dap irng mien djch cua duOT li#u giao co lam [Gynostemma pentaphyllum (Thunb.) IMakino)." T^p chi Thdng tin YDuoc 5 tr 35- 38.
3. Phgm TTianh K^', Phgm T. Huwng, T. L. V. Hien (2007), "Nghien oHi tdc dyng i>c che kh6i u cua saponin chiet ti> gido c6 lam [Gynostemma pentaphyllum (Thunb.) Makino)." Tap chi Thong tin YDu^. 11.
4. Pham Thanh Ky (2006), TTiuc tSp Duoc lieu (phan hoa hgc).
5. Cho. Lee., et al. (2010). 'Physicochemical charactenzatran and NMR assignments of ginsenosldes Rb1, Rb2, Re, and Rd isolated from Panax ginsend' J Ginseng Res., Vo\ 34, No. 2, pp. 113-121 (Ngay nh$n bai: 06/07/2016 - Ngay duyet dSng: 01/09/2016)
Cac hop chit triterpenoid tu- cay buc nau (Mallotus mollissimus (Geisel.) Airy-Shaw)
Phan Minh Giang", Ngo Th| Thu Huyin, Phan T6ng S m
Khoa Hoa hoc, Truang Dai hgc Khoa hQc Tu nhirn. D^l hoc QuSc gia Ha Npi E-mail: phanmiiibgiang@yahoo. comSummary
Keymwtfs: Ma/tote mollissimus, Euphorbiaoeae, Hiteipenad. tamxerane Datvan de
Chi Malhtus (hp Euphorblaceae) c(S si^ 6a dang sinh hoc vi d u tnJc hba hoc. Pham HoSng H« (nam 1999) da IhSng ke 33 ioii Mallotus cOa Vl^t Nam m. Cic nghiSn o i u trong khoSrg 30 n^m cho thay dc toil Malbtus sinh t6ng hyp cac chit chuyen hda thi> c4p thupc cSc nh6m terpenoid steroid, flavonoid, benzopyran. coumahnolignold vi phioraglucinol; cac chat nSy cho mot pho tong do hoat tinh sinh hoc hCru ich nhu- chong ung thir. b i o ve gan, chong oxy hoa ... K. Nhieu loai MalkXus la cac cay thu6c co tniy4n do dd cac hyp chit trong cSc cSy Mallohis ciia Viet Nam rat duwc cltCi y nghien a j u nhu' c i c loai ba bet tiing (M apelta), ba bit nhin (M. glabriusculus), bum bup nau (M paniculatus). ba bst dd (M. metcamanus) m6i khS (M. p«ca(us). cSnh kiln (M. philippensis) ba b i t liin (M. nanus), bum bgp gal (M. barbatus) vi bilm bup bdng to (M. macmstachyus) i«!.
TAP CHi Dir(?C HQC - 9/2016 (S6 485 NAM 56)
cay buc nau (M. mollissimus (Geisel.) Alry,^haw syn. M. riclnokles (Pers.) Miill-Arg.) ciJa Viot Nam chua d u ^ nghien ciXi ve thinh phan hda hpc nhung da co mot s4 cdng bo ve hoat tinh chdng oxy hda va khang ky sinh triing Leismania cCIa M.
mollissimus " i . Bai bao nay lin 6hu Sen cdng b6 cac ket qua phin lap va xac dlnh cau tnic cda 4 chat triterpenoid va p-sitosterol tir ia cay bgc nSu.
Nguyen ll$u va p h u o n g phap n g h B n c i n j Phuomg p h i p va thiet bj
Pho h4ng ngoai (IR) dm?c ghi tren thilt bi Impact^lO-Nicolet FT-IR spectrometer. Pho kh6i lu^ng phun diSn tit (ESI-MS) diryc ghi trdn hs thong LC/MSD Trap Agilen Senes 1100. Cdc ph6 cpng hird'ng ti> hat nhan prpton ('H-NMR 500 MHz) duprc ghi tren thilt bi Bruker Avance 500 v»i tetrametylsilan (TMS) la chit ChuIn nSi zero (5 = 0). Dp chuyln dich hod hoc 6 duyc b i l u thl bang ppm, J tinh theo Hz. S i c ky I6p mdng
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