• Nghien
CLPU- Ky thuat
proprietary Ciproxin tablets and generic ciprofloxacin tablets using handheld Raman spectroscopy", Joumal of Raman Spectroscopy, 43(8), pp. 1049-1057.
3. Degardln Klara, Roggo Yves, Margot Pierre (2014), "Understanding and fighting the medicine counterfeit mari<et", Joumal of Phannaceutical and Biomedical/Analysis, 87, pp. 167-175.
4. Hajjou Mustapha, Qin Yanyan, Bradby Sanford, Bempong Daniel, Lukulay Patrick (2013), "Assessment of the performance of a handheld Raman device for potential use as a screening tool in evaluating medicines quality", Joumal of Pharmaceutical and Biomedical Analysis, 74, pp. 47-55.
{Ngay nhan bai: 06/11/2015 - Ngay duyet dang: 06/01/2016)
Nghien cu'u tac dung tang lye
cua che pham TDOOOl tren thyc nghiem
Le Thi Ngoc Diep, Tran Thanh Tung*, Pham Thi Van Anh Bg mon Dugc ly — Trucmg Dgi hgc Y Hd Ngi
' E-mail: maiphuong2005'a)gmail. com
Summary
Objective; The preparation TDOOOl was investigated for the enhancing effect on the physical strength In Swiss mice of both sexes by 3 experimental models: Forced swimming endurance test, rotarod performance test and grip-strength test The animals were divided into four groups: I - control;
ll - positive control (received Arcallon (sulbutlamlne) 96 mg/kg); III & IV - treated with TDOOOl 10.8 ml/
kg (equivalent clinical dose) and 32.4 ml/kg (three-time clinical dose). At the doses of 10.8 ml/kg and 32.4 ml/kg, TD0001: (1) Increased swimming endurance time without any increase in blood glucose and albumin levels after 2 weeks In swimming endurance tests; (2) Increased both the duration of stay on Rotarod apparatus and the strength of mice in grip-strength tests after 5 days. Thus, TDOOOl at the tested doses (equh/alent to and three-fold the clinical dose) enhanced the physical strength of Swiss mice. The effects were significantly similar in both treatment groups.
Keywords: Preparation TDOOOl, Swiss mice, enhancing physical strength effect, experiment
Oat van de
Suy nhuge ca t h i la tinh trang suy nhuge phirc tap dugc dac trung bai met mdi keo dai khdng dugc cai thien khi nghi ngai, cac trieu chirng ed t h i trim trpng han sau hoat ddng t h i lue hoac nhu'ng sang chin v l tinh thin. Trieu chirng ehinh eiia benh bao gdm met mdi keo dai, ngii khdng ngon giie, dau diu, kem tap trung va suy giam tri nhd, an ulng khdng ngon mieng, dau khap, dau ca, y l u ea gay anh huang Idn din cupc sdng hang ngay ciia benh nhan. Tang luc bao g i m cac thao duge hoac thulc sii' dung tang cudng su'c d l khang ciia ca t h i vdi stress, chin thuang, met mdi, diiu trj su mit can bing cua ca t h i , thiic diy he thing miln djch, tang nang lugng va tinh thin minh min. Adaptogen
TAP CHI Dl/OC HOC - 1/2016 (S6 477 NAM 56)
dugc ehl djnh eho benh nhan duge chan doan suy nhuge ea t h i . Theo Trung tam kilm soat djch benh IVIy, nam 2008 tai nudc nay ed han mdt trieu ngudi mie suy nhuge ca t h i , x i p xl 80%) trong s l dd khdng dugc chin doan. Tai Anh, theo bao cao eiia Djeh vu sire khde quic gia (National Health Service), s l ngudi bj anh huang bai tinh trang nay la 250.000 ngudi " l Cdn tai Viet Nam, ty ie nay ia 3-4% dan s l trong cpng ddng. Nguyen nhan ciia tinh trang nay d i n nay van chua dugc lam sang td, nhung eae nha khoa hpc cho ring tinh trang nhiim trung, rdi loan v l miln djch, stress, chin thuang va ngd ddc cd t h i la y l u to thuc diy. Hien tai chua cd bien phap dac hieu d l diiu trj. Cae bien phap ehii y l u la luyen tap t h i due, nang cao sire d l khang cua ea the... Them vao dd, benh nhan cd t h i sir dung
• Nghien CLPU - Ky thuat
them mdt s l thulc d l cai thien trieu chirng ttf dd nang eao sire khde. Tren t h i gid-i da cc nhilu nghien ciru v l tac dung tang lue eiia mdt s l loai dugc lieu nhu nghien ciru ciia S. Aydin v l A. pallida va T. argentea, nghien ciru cila F.
Wiegant v l £. senticosus va R. rosea ,...i^^'. Tai Viet Nam, nhilu bai thulc y hpc co truyin giup tang eudng sire khde nhu "Bat tien trudng thp hoan", "Thap toan dai bl",... da duge irng dung tren lam sang. C h i phim TD001 dua theo bai thule CO phuang la "Sam linh bach truat hoan"
thanh phin bao gdm: dang sam, bach truat, phuc iinh, hoai son, lien nhuc, y dT, cam thao bie gia them san tra, maeh nha va thin khuc. Bai thulc c l phuang nay da dugc dung tren lam sang vdi tac dung phuc hdi ca t h i sau suy nhuge, nhung cho din nay chua cd mdt nghien ctru nao chirng minh tac dung nay w. Vi vay chung tdi tiln hanh d l tai nhim muc tieu: Banh gia tac dung tang luc ciia ehl phim TDOOOl tren chupt nhit trang ehiing Swiss.
Nguyen lieu, ddi tu'O'ng va phu'ang phap nghien cu'u
Nguyen lieu
- Thudc nghien ciru: c h i phim TDOOOl cua Cdng ty C l phin Sao Thai Duang, dang Ip dung dich 100 ml bao gim: dang sam 8 g, bach truat 8 g, phiic linh 12 g, hoai san 8 g, lien nhuc 8 g, y dT 12 g, cam thao bae 4 g, maeh nha 12 g, son tra 4 g va thin khtle 12 g. IVIdi Ip chira 556 g dupc lieu.
Thuoc chimg dirang: Arcallon (biet dugc eua sulbutiamin) ciia Hang Les Laboratoires Servler Industrie, Phap.
- Dung cu nghien ciru: True quay Rotarod 7650, may do su'c keo 7106 - Hang Ugo-Basile, Italy May dinh lugng dudng huylt On Call EZ II - Hang Aeon - My.
Opng vat nghien ciru
Chudt nhit tring chiing Swiss, ea 2 gilng khde manh, trpng lupng 23 + 2 g do Vien Ve sinh Djch t i Trung uang cung cip. Dong vat dugc nudi 5-10 ngay trudc khi nghien cu-u va trong suit thdi gian nghien ciru trong dllu kien phdng thi nghiem vd-i diy du thirc an va nudc ulng tai Bd mdn Duge iy - Trudng Dai hpe Y Ha Ndi.
Phu'O'ng phap nghien cu'u
Nghien ciru anh huv'ng cua TD0001 tren mo hinh chugt bo'i
Dua theo md hinh chupt bai ciia Brekhman 1980 i^'^i, ehupt dugc chia ngiu nhien thanh 4 Id:
- Ld 1: (chu'ng, n = 10) uong nud-e muli sinh ly 0,2 ml/10 g.
- Ld 2: (n 10) ulng Arcalion lilu 96 mg/kg (Lilu tuang duang lam sang, he s l ngoai suy 12).
- Ld 3: (n = 10) ulng TD0001 lilu thip 10,8 ml/kg (Tuang duang lilu lam sang, he s l ngoai suy 12).
- Ld 4: (n = 10) ulng TDOOOl lilu cao 32,4 ml/
kg (Gip 3 lin tren lam sang).
Ngay ulng 1 lin vao buoi sang trong 2 tuin lien tuc. Theo ddi thai gian bai trudc khi ulng thulc, sau uong thulc 2 tuin. Thdi gian bai dugc tinh ttf lue eho chupt vao binh nudc d i n khi ehudt chim trong nudc 8 giay. Ngoai danh gia thai gian bai, cac chupt sau khi bai l i n euli duge lay mau de djnh lupng glucose, albumin.
Nghien ciru anh huxrng cua TD0001 len SU'C bam cua chugt
Dua theo md hinh true quay Rotarod ciia Dunham Mlya i=i, chia Id nhu muc 'Nghien ciru anh hw&ng cua TD0001 tren mo hinh chupt bo'i", ngay ulng 1 lin vao bull sang trong 5 ngay lien tuc. Theo ddi cac chi tieu 6- 2 thdi dilm: trud-c nghien ciru (TO) va sau ulng thulc 5 ngay (T1), ngay thu' 5 hoat ddng dugc ghi a thai dilm 1 gid- sau ulng thudc. Tai m i l thdi diem nghien ciru, tiln hanh theo doi thdi gian bam eiia chudt (ttf khi dat len cho tdi khi chudt rai khdi true quay).
Kit qua dupe so sanh su khac nhau v l thdi gian bam tren true quay.
Nghien ciru inh hwdng cua TDOOOl len sire keo cua chugt
Dua tren md hinh do su'c keo ciia Cabe v^
cdng su R, chia Id nhu muc "Nghien ciru anh hwdng cua TD0001 tren mo hinh chugt bo'i", ngay ulng 1 lin vap bull sang trong 5 ngay lien tuc. Theo ddi cac ehl tieu 6' 2 thdi diem nghiin cu-u: trudc nghien ciru (TO) va sau ulng thulc 5 ngay (Tl), ngay thir 5 hoat ddng dugc ghi d- thdi diem 1 gid- sau ulng thule. Tai mdi thd-i dilm:
chupt nhit dupe dat len dTa Perpex ciia thilt bj 12 TAP CHI DUQC HOC - 1/2016 fSO 477 NAM 561
Nghien CLPU - Ky thuat
do sire bam. Dat chudt dau hudng v l phia tay va test trudc sau (Avant-apres). Bleu d i l n dang n i m , khi keo dudi chupt ngupc lai thi thee phan X ± SD.
K i t qua nghien ciru
xa chudt se bam vao tay nam de chdng iai, khi dd SU'C keo t i i da nay se d u g c bd nhd- ghi luu lai.
Xu" ly s6 lieu ^^'- "^""S '^"^ TD0001 tren mo hinh chupt bo'i
Cac sd lieu thu thap d u a c xif ly bang phuang
phap t h i n g ke y sinh hpc theo t- test - Student ^"^ hwdng cua TD0001 den th&i gian bo'i Bang 1 : Su thay ddi thdi gian bai ciia chudt sau udng thudc 2 tuin
STT ^^'^'°.
diing thuoc 1 NaCI 0,9%
2 Arcalion 96 mg/kg 3 TD0001 10,8 ml/kg 4 TDOOOl 32,4 ml/kg
n
10 10 10 10
Thoi Tmuc udng thudc
104,67 ±33,75 102,75 ±24,53 105,87 ±36,44 97,44 + 30,86
gian boi (giay) Sau uong thuoc 2 tuan
121,92 ±42,58 160,18 ±50,99 163,83 ±44,32 155,46 ±39,42
% thay doi so v&i tnr&c diing thuoc
116,48 155,89 154,75 159,54
p so v&i trw&c uong thuoc
<0,01
< 0,001
< 0,001
< 0,001
p so v&i 161
<0,05
<0,05
<0,05 Sau 2 t u i n u l n g thulc, Arcalipn va thulc thu- tuang d u a n g nhau va tuang d u a n g vdi Arcalion ca 2 lilu d i u lam tang thai gian bai (p < 0,001). l i l u 96 mg/kg vai p > 0,05.
C h i p h i m TD0001 a ca 2 lilu 10,8 ml/kg va ^„fj hwdng cua TD0001 len nong dp 32,4 ml/kg lam tang thai gian bai ciia ehupt tupng glusoce va albumin trong mau chupt ngay irng la 34,37 va 27,51%), tae dung ciia 2 l i l u sau bo'i
Bang 2: Ham luung albumin STT Cac Id diing thuoc
1 NaCI 0,9%
2 Arcalion 96 mg/kg 3 TD0001 10,8 ml/kg 4 TDOOOl 32,4 ml/kg
va glucose mau ngay sau boi tai thdi diem sau udng thudc 2 tuan n
10 10 10 10
Albumin (g/dl) 3,49 ± 0,49 3,70 ± 0,72 3,78 ± 0,64 4,01 ± 0,86
Glucose (mmol/l) 7,79 ± 0,94 8,41 ±1,26 7,76 ±1,16 8,38 ±0,71
p so v&i 1
>0,05
>0,05
>0,05
>0,05 N i n g dp glucose va ham lugng albumin d sau 2 t u i n dting thulc.
cac Id dung Arcalion, TDOOOl lieu 10,8 ml/kg va j a c dung cua TD0001 tren mo hinh true 32,4 ml/kg khdng cd s u thay doi so vdi id chirng quay Rotarod
Bang 3: Su thay ddi thdi gian bam cua chudt
STT ^^'^'°.
diing thuoc 1 NaCI 0,9%
2 Arcallon 96 mg/kg 3 TDOOOl 10,8 ml/kg 4 TDOOOl 32,4 ml/kg
11
10 10 10 10
Th&i gian bam (giay) Tnr&c uong thuoc
222,45 ±62,13 232,91 ± 54,25 239,08 ± 67,08 224,62 ±61,69
Sau udng thudc 5 ngay 233,64 ± 70,73 303,00 ± 78,28 322,00 ± 55,53 288,15 ±52,88
% thay doi so v&i 16 chirng
t29,69 t37,82 t23,33
p so v&i trw&c uong thuoc
>0,05
<0,05
<0,01
<0,05
p so v&i 161
<0,05
<0,01
<0,05 Arcallon va thule thu ca 2 lilu d i u lam tang ciia chudt tuang irng la 37,82 va 23,33%, tac dung rd ret sire bam ciia chupt so vdi thdi d i l m trudc ciia 2 l i l u TDOOOl tuang duang nhau va t u a n g nghidn cu'u, s u khac biet ed y nghTa t h i n g ke. d u a n g vdi Arcalion 96 mg/kg vdi p > 0,05.
C h i p h i m TDOOOl d ca 2 lilu lam tang sire bam jac dung cua TD0001 tren mo hinh do su'c keo Bang 4: Su thay ddi site keo ciia chupt
g j ^ Cac 16 Sipc keo (g) % thay doi so v&i p so v&i trum p so v&i dung thuoc Tnrdc udng thudc Sau uong thuoc 5 ngay 16 chirng uong thuoc 161 1 NaCI 0,9% 10 219,08 ±44,48 242,64 ±48,53 >o,05 2 Arcalion 96 mg/kg 10 233,92 ±48,24 285,90 + 42,99 ]17,83 < 0,05 < 0,05
3 TDOOOl 10,8 ml/kg 10 21217 ±39,02 264,58 ±73,61 19,04 <0,05 >0 05 4 TD0001 32,4 ml/kg 10 213,38 ±53,07 256,69 ±44,20 |5,79 < 0,05 > 0,05
TAP CHI Dl/OC HOC - 1/2016 (SO 477 NAM 56^ ]3
• Nghien
CLPU- Ky thuat
Arcalion va thule thu ea 2 lilu lam tang sire keo cua chudt. TDOOOl d ca 2 lilu lam tang sire keo eua ehudt tuang irng la 9,04 va 5,79%, tae dung cua 2 lilu TD0001 tuang duang nhau va tuang duang vdi Arcalion vdi p > 0,05.
Ban luan
Chi pham TDOOOl dugc san xuit dua theo bai thule eo phuang "Sam Ilnh bach truat tan" gia giam. Tren md hinh chudt bai, TD0001 lilu 10,8 va 32,4 ml/kg lam keo dai thd-i gian bai cua chupt sau khi ulng thulc lien tuc trong 2 tuin, thdi gian bai tang them tuang irng la 54,75 va 59,54%, tac dung giu-a hai lilu tuang duang nhau va tuang duang vdi Arcalion lilu 96 mg/kg (tang 55,89%).
Ning dp glucose va ham lupng albumin trong mau chupt ngay sau khi bai khdng khac biet gitra cac Id. Theo kit qua trong nghien ciru ciia R.
Duraisami v l tac dung ching stress va tang luc cua biu nau {Aegle marmelos) tren chudt cing, biu nau lilu 200 va 400 mg/kg dung trong 7 ngay ed tac dung keo dai thdi gian bai tuang irng la 91,6 va 163,2%. Trong ngay diu sau khi ulng biu nau ning dp glucose va ham lupng albumin cd tang len, nhung nhu'ng ngay sau trd v l binh thudng, theo tac gia nong dp cac chit nay khdng thay dii la do ca ehl tu dllu hda eiia ca the P'.
Tren md hinh true quay Rotarod: TDOOOl lilu 10,8 va 32,4 ml/kg da keo dai thdi gian bam eiia ehudt sau khi ulng thulc lien tuc trong 5 ngay, thdi gian bam tang them 37,82 va 23,33%, tuang duang vdi Arcallon lilu 96 mg/kg (tang them 29,69%). Tren md hinh sire keo: TDOOOl lilu 10,8 va 32,4 ml/kg da lam tang sire keo eua ehudt sau khi ulng thulc lien tuc trong 5 ngay sire keo eiia chupt da tang them 9,04 va 5,79%, tac dung tuang duang vdi Arcalion lilu 96 mg/kg (tang them 17,83%). So sanh kit qua tren md hinh true quay Rotarod va dp sire keo vdi nghien ciru cua Fulvio Rieli Mendes v l tac dung tang luc eiia thao dupc vung Nam My ed ten khoa hpc la Baccharis trimera vdi lilu 40 va 200 mg/kg va Davllla rugosa lilu 20 va 100 mg/kg trong 45 ngay kit qua cho thiy ca hai thao dugc nay diu khdng the hien tac dung tang luc '^i.
TDOOOl bao gdm cae vi thulc bo khi kien ty, dud'ng vj nhu: dang sam, hoai san, lien nhuc, maeh nha ed tae dung bo khi, kien ty, dudng vj, gliip tieu hda, hip thu thirc an va qua trinh van chuyen tan dich. Khi cdng nang cua ty vj dugc eudng kien.
thi ca t h i mau ddi, muon an va an ngon mieng han. hoai san va thin khuc, ngoai t^c dung ly khi hda thip chu'a dau bung, day bung. ThO'c an ulng vao vi nhd' sir van hda, hip thu ciia tyf de phat trien ca nhuc. Ngpai ra hoai san, y dT, lien nhuc cd thanh phin ehii ylu la glucld, lipid, protein, vitamin A, B, C va mdt s l ylu td vi lugng quan trpng nhu sat, calci, phospho giup eg the phat triln, nang cao sire d l khang.
Kgt luan
Tren md hinh chudt bgi: TD0001 lilu 10,8 va 32,4 ml/kg ulng lien tuc trpng 2 tuan ed tac dung lam tang cudng sire b i n , the hien qua viec lam kep dai thdi gian bai, khdng anh hup'ng din ning dp glucose va albumin trong mau chudt sau khi bai.
-Tren md hinh true quay Rotarod: TD0001 lilu 10,8 va 32,4 ml/kg ulng lien tuc trong 5 ngay cd tac dung lam tang su'c luc the hien qua viec lam tang cudng thdi gian bam tren true quay
- Tren md hinh do su'c keo ehupt: TD0001 lilu 10,8 va 32,4 ml/kg ulng lien tuc trong 5 ngay cd tac dung lam tang su-c luc the hien qua viee lam tang eudng su'c keo.
Tai lieu tham khao
1 G. Ranjith (2005), "Epldermiology of chronic fatigue syndrome". Occupational Medicine, Vol. 55, pp. 13-19.
2. Wiegant F. A. C, Surinova S., Ytsma E., Langelaar-Makkinje M., Wikman G., Post J. A. (2008),
"Plant adaptogens Increase lifespan and stress resistance in C. Eiegans", Biogerontology, 10 (1), pp.
27-42.
3. R. Duraisami, Vijay A. Mohite, Amit J. Kasbe (2010), "Anti stress, adaptogenicaclivity of standardized dried fruit extract of Aegle marmelos against diverse stressors", Asian Journal of Pharmaceutical and Clinical Research, Vol. 3, Issue 4, pp. 1-3.
4. Pham Van Bay (2008), "Nghien ciru tac dung ctia bai thuoc "Sam linh bach truat tan" trong dieu trj chirng hu lao a nguoi cao tuoi", Luan van Chuyen khoa 11, Bai hoc Y Ha m\.
5. Ngd Danh Luc (2009), "Danh gia tac dung cua bai thulc nhan sam dud'ng vinh thang dllu tri chu-ng hu lao the tam ty hu", Luan van tiin sTY hoc, Truong Dai hpc Y Ha Npi.
6. Fulvio Rieli Mendes (2007), "Evaluation of Baccharis trimera and Davllla mgosa in tests for adaptogen activity". Phytotherapy Research, 21, 517- 522.
{Ngay nhan bai: 16/11/2015 - Ngay duyet dang: 06/01/2016)
14 TAP CHi DU'OC HOC - 1/2016 fSO 477 NAM 561
