P CHl" Y DLf PC Hpc CAN THP

T»P CHl" Y DLf PC Hpc CAN THP - SO 11-12/2018 TAI L I E U T H A M K H A O

1. Arana M., et al. (2013), "Adipose tissue-derived mesenchymal stem cells: isolation, expansion, and characterization", Cellular Cardiomyoplasty: Methods and Protocols, pp. 47-61.

2. Amhold S. and Wenisch S. (2015), "Adipose tissue derived mesenchymal stem cells for musculoskeletairepairin veterinary medicine", j4mer/c(3nyouraa/o/'5(emce//.y. 4(1), p. 1.

3. BaerP.C. and GeigerH. (2012), "Adipose-derived mesenchymal sttomal/stem cells: tissue localization, characteri2ation, and heterogeneity", Stem cells international. 2012.

4. Banas A., et al. (2007), "Adipose tissue-derived mesenchymal stem cells as a source of human hepatocytes", Hepatology. 46(1), pp. 219-228.

5. Bunnell B.A., et al. (2008), "Adipose-derived stem cells: isolation, expansion and differentiation". Methods. 45(2), pp. 115-120.

6. Dominici M., et al. (2009), "Heterogeneity of multipotent mesenchymal sttomal cells:

from sttomal cells to stem cells and vice versa", Transplantation. 87(9S), pp. S36-S42.

7. Frese L., Dijkman P.E., and Hoerstrap S.P. (2016), "Adipose tissue-derived stem cells in regenerative medicine". Transfusion Medicine andHemotherapy. 43(4), pp. 268-274.

9. Kim N, and Cho S.-G. (2013), "Clinical applications of mesenchymal stem cells". The Korean journal of internal medicine. 28(4), pp. 387-402.

lO.MaUam E., et al. (2010), "Characterization of in vitto expanded bone marrow-derived mesenchymal stem cells from patients with multiple sclerosis". Multiple Sclerosis.

11.Meirelles L.d.S. and Nardi N.B. (2003), "Murine marrow-derived mesenchymal stem cell:

isolation, in vitto expansion, and characterization", British journal of haematology.

123(4), pp. 702-711.

12.Mohammadi-Sangcheshmeh A., et al. (2013), "Isolation, characterization, and mesodermic differentiation of stem cells from adipose tissue of camel (Camelus dromedarius)". In Vitro Cellular & Developmental Biology-Animal. 49(2), pp. 147-154.

13.Niyaz M., et al. (2012), 'Isolation, cuhuring and characterization of rat adipose tissue-derived mesenchymal stem cells: a simple technique", Turkish Journal of Biology. 36(6), pp. 658-664.

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(Ngdy nhgn bai: 10/11/2017- Ngay duyet ddng: 08/01/2018)

BON HOP C H A T P H A N LAP TU" T H A N C A Y BBVI BIP CLINACANTHUS NUTANS (BURM. F.) LINDAU, A C A N T H A C E A E

Nguyin Thi Trang Ddi'*, Phan Nggc Vdn Anh', Huynh Nggc Thu/

I. Trudng Dgi hgc YDugc Cdn Tha 2. Bgi hgc YDugc TP. Ho Chi Minh 'Email: dai ngiryenthiti-ang(^yahoo.com

TOM T A T

Dgt v^n di: Sim hip tir Idu da dugc xem la vj thudc eo truyin a Thdi Lan, Indonesia, Malaysia. Y hgc ddn gian cua cdc nude dd ghi nhgn, Bim bip cd tdc dung chdng oxy hda. khdng khudn, khdng viem, trj cdn trimg^ eon, sdt, ban da, ly. ddi thdo dudng. Muc tiiu nghiin ciru: Phdn lgp mgt sd ftpp ehdt tir dich chiet thdn Bim bip vd xdc djnh cdu true cdc hgp chdt ndy. Doi tugng

379

TAP CHI Y Dl/QfC Hpc c X N T H g - s 6 11-12/2018

vd phuang phdp nghiin cd:u: Chiit ngdm kiet bgt thdn vdi cdn 96%. Bdng ky thugt chiit phdn bS Idng - long vdi dung mdi cd do phdn cgc tdng ddn, thu dugc ede phdn dogn cao chiet dichloromethan vd ethyl aeetat Ung dmg ky thugt sde ky cot di phdn lap cdc hap chdt. Cdu trite hda hgc cua cdc hgp chdt ndy dugc xdc dinh bdng kp thudt pho MS, NMR. Kit qud: 10 kg hot thdn Bim bip khd thu dugc 56,68 g cao dichloromethan vd 15,18 g cao ethyl aeetat. Tir cao dichloromethan dd phdn lgp duge 4 hgp ehdt CN6-2 (stigmasterol), CN25-2 (isoliguiritigenin), CN25-3 (a. 4, 2' 4'-tetiahydroxydihydroehalcon), CN25-4 (glycerin 1-monotricosanoat) Ketlu^n:

Kit qud ndy cd thi tgo ca sd cho viic nghiin ciru tdc dung sinh hgc cita thdn Bim bip vd sir dung cdc chdt ndy phyc vu eho cdng tdc kiim nghiim, tiiu chudn hda chdt luang cdc sdn phdm va ngityen liiu chira djch chiit than Bim bip.

Tir khda: Bim bip; Isoliguiritigenin, a,4,2'.4'-tetrahydroxydihydrochalcon. Glycerin 1- monotricosanoat, stigmasterol

A B S T R A C T

F O U R C O M P O U N D S I S O L A T I O N F R O M S T E M S CLINACANTHUS NUTANS ( B U R M . F.) L I N D A U , A C A N T H A C E A E

Nguyen Thi Trang Dai', Phan Ngoc Van Anh', Huynh Ngoc Thu/

Cantho University of Medicine and Pharmacy HoChiMinh University of Medicine and Pharmacy Background: Clinacanthus nutans as considered as a traditional medicine plant in Thaland, Indonesia, Malaysia. Traditional medecine has noted that Clinacanthus nutans has antioxidant, antibacterial and anti-imflammatory. Activities treatment for insect bite, fever, sldn ras and diabetes Objective: Isolation and determination structure of compound from stems of Clinacanthus nutans Method: The Clinacanthus nutans stems were perolated with 96% ethanol, by liquid-liquid distributing extraction with graduated solvent. Obtained fractional extract dichloromethan and ethyl aeetat, purified by using conventional column chromatography. The chemical structures of the isolated compounds were elucidated by with MS and NMR technique.

Results: from 10kg of dried stem of Clinacanthus nutans, 56,68 g of dichloromethan fraction and 18.15 g ethyl aeetat fraction was obtained. From this fraction, four compounds CN6-1. CN6-2, CN12-1 were isolated. The elucidated structure of these compounds were methylcosanoat, stigmasterol and n-octacosanoic respectively. Conclusion: The results offer the reference standards for Clinacanthus nutans stems extract which can be used for further research on its biological effects or preparation of therapeutic medications.

Keywords: Clinacanthus nutans, Isoliquiritigenin, a,4.2',4'-tetiahydroxydihydrochalcon, Glycerin 1-monotiicosanoat. stigmasterol

I. D ^ T VAN D E

Bim bip (Clinacanthus nutans (Burm. f.) Luidau, Acanthaceae) tir lau da dugc xem Id vi thudc cd truyen d Thdi Lan, Indonesia, Malaysia. Y hgc ddn gian cua cac nude da ghi nhan, bun bip cd tdc dung chdng oxy hda, khang khudn, khdng viem, dOc tS bao [2],[5], hi cdn triing cdn, sdt, ban da, ly, ddi thao dudng [1],[4]. Tai ViSt Nam, chua cd nhiing bao cdo nghien ciru sau v l tdc dung sinh hgc vd hda hgc tii cay bim bip. Tron^ bai bdo ndy bao cdo ket qud budc ddu phan l^p va xac dinh cdu tnic hda hgc cua 4 hgp chat tmh khilt phan lap dugc tir than cay bim bip (Clinacanthus nutans (Burm.f.) Lindau) mpc tai ViSt Nam.

n . D O I TU*CflVG VA PHUOfNG P H A P N G H I E N ClTU 2.1. Ddi tu-gng nghien cihi

380

TAP CHf Y DLTgC HQC CAN T H g - SO 11-12/2018

Loai bim bip nghien ciiu dugc thu hai d mii Cdm, Chau Ddc, An Giang vao thdng 12/2015. Mau nghiSn ciiu dugc TS. Vd Van Chi giam dinh tSn khoa hpc Clinacanthus nutans (Brum, f.) Lmdau, hp 6 rd (Acantiiaceae), mang sd hieu Bbl215 vd dugc luu tai Phdng Tieu ban thuc vat, BO mdn Dugc lieu, khoa Dugc, DH Y Dupe TP. Hd Chi Minh.

2.2. Phirong phap nghien ctiu Phirang phdp phan lgp cdc hgp chat

Chiet ngam kiSt bgt thdn vdi cdn 96% thu dugc cao chiit toan phdn. Bdng ky thuat chiet phan bo Idng - Idng vdi dung mdi cd dp phan cue tang d^, thu dugc cac phdn doan cao chiSt dichloromethan va ethyl aeetat. Sau dd dung ky thudt sdc k^ c^t dl phdn l|.p cdc hgp chat, sdc ky cdt dugc tiin hanh vdi silica gel pha thudn (0,040 - 0,063 mm, Merck).

Theo ddi cdc phdn doan sdc ky bdng sdc ki lop mdng (SKLM), dugc thuc hien ttSn bdn mdng ttdng sin DC-AlufoIien 60 F254 (Merck) (silica gel, 0,25 mm). Phdt hien chdt bdng den tii ngoai d hai budc sdng 254 nm vd 365 nm, dung tiiuoc thti Id dung dich vanillin 1%

ttong cdn tuyet ddi va dung dich H2SO4 5% ttong cdn tuyet ddi.

Phmmg phdp xdc dinh cau true cdc hgp ch&t

cau tnic cac hgp chat phan lap dupe dga tten cac thdng sd v$t ly va cac phuang phap phd bao gdm: phd khdi lugng (MS), phd cdng hudng tir hat nhan (ID-, 2D-NMR) va so sanh dir lieu phd thu dugc vdi cac dii lieu phd da cdng bd. Phd MS dugc do ttSn nmy Agilent 1100 LC/ MSD Trap SL, phd cdng hudng tii hat nhan dugc do ttSn may Broker AM500 FT-NMR. Chdt ndi chudn la tettamethyl silan tai Vien Hda hpc, ViSn Hdn ldm Khoa hpc va Cdng nghe ViSt Nam.

III. KET QUA VA BAN LUAN Chiit xuat vaphan lgp

Nguyen lieu la 10 kg bgt than Clinacanthus nutans dugc chiSt ngam kiet vdi 90 lit ethanol 96% cho dSn khi hSt chdt hda tan ttong dung mdi. Lay 300 g cao chiSt cdn 96%

chiSt phdn bd Idng - long ldn lugt vdi cac dung mdi cd do phan cue tang dan: 6 lit dichloromethan va 4 lit ethyl aeetat. Loai dung mdi, thu dugc 56,68 g cao dichloromethan va 15,18 g cao ethyl aeetat dimg phan lap.

Phan igp cao dichloromethan

Cao dichloromethan tiin hanh sdc ky cot pha thudng vdi silica gel cd h^t vira ( Merck; 0,4-0,63 |im) dung moi rua giai la dichloromethan, sau dd tang dan do phan cue bdng each su dung he dung mdi dichloromethan-methanol, bat dau bdng ty IS (99:1) sau dd tdng dan ty IS methanol tach thdnh 34 phan dogn (CNl-34).

Tir phdn doan CN6 va CN25 tiep tuc dugc tiin hanh sac k;^ c^t pha thuang vdi silica gel cd hat vira (Merck; 0,4-0,63 pm), dung mdi nia giai la n-hexan-ethylacetat vdi ty IS ethylacetat tang ddn. Phan doan CN6 thu dugc 1 chdt kit tinh CN6-2 (10 mg), phan doan CN25 tiiu dugc 3 chdt kit tinh Id CN25-2 (10 mg), CN25-3 (6 mg) vd CN25-4 (18 mg). Lam sach cdc chdt nay bdng kit tinh ttong dung mdi tiiich hgp.

Xac dinb cau trAc cdc hgp chdt Hop chat CN25-2

Tinh till hinh kim, mau ttdng, ESI-MS m/z: 256,7 [M+H]^, 254,8 [M-H]" 'H-NMR (CD3OD, 500 MHz) 5H: 7,81 (IH, d, J=15,5 Hz, H- p), 7,98 (IH, d, J=9,0 Hz, H-6'), 7,65 (2H, d, J=2,0 Hz, H-2, H-6), 7,62 (IH, d, J-16,5 Hz, H- a), 6,88 (2H, d, J- 9,5 Hz, H-3, H-5), 6,44 (IH, dd, J=2,5, 9,0 Hz, H-5'), 6,32 (IH, d, J-2,0 Hz, H-3'); "C-NMR 381

TAP CHi Y DUgC HQC (^K THg - s6 11-12/2018

(CD3OD, 125 MHz) 5c: 193,5 (C=0), 167,5 (C-4'), 166,3 (C-2'), 161,5 (C-4), 145,6 (C- p), 133,3 (C-6'), 131,8 (C-2, C-6)), 127,8 (C-1), 118,3 (C-a), 117,0 (C-3, C-5), 114,7 (C- 1'), 1 0 9 , 1 ^ 5 ' ) , 103,8 (C-3').

Phd khdi ESI-MS cua hop chdt CN25-2 cho pic ion phan tii d m/z: 256,7 [M+H]*, 254,8 [M-H]" cho phep gpi y cdng thiic phan tii la: C15H12O4 cd M = 256, ggi y day Id mpt flavonoid.

Phd '^C-NMR cua hgp chat CN25-2 cho thdy 15 tin hieu cdng hudng cua carbon ttong do c6 6 carbon bac IV, 9 carbon bac III, khdng cd tin hieu cua carbon bac II va bac I.

Trong sd do cac carbon bac IV cd dp dich chuyen hda hgc 5c tai 193,5 ppm; 167,5 ppm;

166,3 ppm; 161,5 ppm d vimg trudng thap dac tnmg cho cac carbon lai hda sp^ va cd gdn di td cd dp am dien ldn thdng thudng Id oxy. Carbon bac IV cd 5c tai 193,5 ppm la nhdm C=0 cua vdng C. Cdn lai 2 carbon bac IV vdi dp dich chuyen hda hgc 5c 127,8 ppm;

114,7 ppm la cac carbon bac IV mang n6i ddi cua vdng tham. Trong sd cdc carbon bac III cd 2 tin hieu kep (# 4 CH) d 5c 131,8 ppm vd 117,0 ppm, chiing td cd su ddi ximg va sir doi xiing chi cd the tten vdng B, 4 CH ddi xiing nay thudc vdng B (2/6 va 3/5), chiing td vong B cd nhdm the thdng thudng Id hydroxy. Hai proton ddi xiing 6H 7,65 ppm va 6,88 ppm cd tuong tac HMBC vdi 8c 161,5 ppm, nhdm the -OH gan vao carbon ndy (C-4).

Hai carbon bac IV 5c 167,5 ppm va 166,3 ppm cd gdn vdi di td co do dm dien lon thdng thudng la hydroxy. Cac carbon bac III, 1 carbon b^c III cd 5c 133,3 ppm, gid tri 8H Id 7,98 ppm la mpt dinh ddi (d) cd t u a i ^ tdc vdi hdng sd ghep J = 9,0 Hz diSu nay cho tiidy proton nay tuang tac oriho vdi mgt proton khac, 1 carbon bac III cd 5c 103,8 ppm vdi gid tri 5H 6,32 ppm Id mdt dinh ddi (d) cd tuong tdc vdi hang so ghep J= 2,0 Hz, diiu nay cho thdy proton nay cd tuong tac meta vdi mpt proton khdc, 1 carbon bdc III cd 5c 109,1 ppm cd gid tti 5H 6,44 ppm Id hai dinh ddi (dd) cd tuong tdc vdi hdng sd ghep Jj = 9,0 Hz vd J2 = 2,5 Hz, chiing td proton ndy cd tuong tdc ortho vdng tiiom vdi proton 5H la 7,98 ppm va tuong tdc meta vdng thom vdi proton 6,32 ppm.

Tir kSt qud phdn tich phd ID, 2D~NMR, MS, kit hgp vdi so sanh tdi liSu [5] cd thi ket luan hop chdt CN25-2 la isoliquiritigenin,

Hgp chat CN25-3

Tiki till hinh kun mau ttdng nga. ESI-MS m/z: 297,3 [M+Na]^, 254,8 [M-H-H2O]"

. 'H-NMR (CD3OD, 500 MHz) 5H: 7,75 (IH, d, J=8,5 Hz, H-6'), 7,34 (2H, d, J=8,5 Hz, H-2, H-6), 6,84 (2H, d, J=8,5 Hz, H-3, H-5), 6,52 (IH, dd, J=2,0 Hz, 8,5 Hz, H-5'), 6,37 (IH, d, J=2,5 Hz, H-3'), 5,40 (IH, dd. J=2,5 Hz, 13,0 Hz, H-a), 3,06 (IH, dd, J=13,0 Hz, 16,5 Hz, H-a), 2,71 (IH, dd, J=3,0, 17,0 Hz) '^C-NMR (CD3OD, 125 MHz) 5c: 193,5 (C=0), 166,7 (C-4'), 165,5 (C-2'), 159,0 (C-4), 131,3 (C-1), 129,8 (C-6'), 129,0 (C-2, C- 6), 116,3 (C-3, C-5), 115,0 (C-1'), 111,7 (C-5'), 103,8 (C-3'), 81,0 (C-a), 45,0 (C-p).

Phd khdi ESI-MS cua hpp chdt CN25-3 cho pic ion phdn tii d m/z: 297,3 [M+Na]"", 254,8 [M-H-HaO]-, cho phdp ggi y cong tiiuc phan tii la: CisHuOj cd M = 274 ggi y ddy la mot flavonoid.

Phd C-NMR ciia hgp chdt CN25-3 cho thdy 15 tin hieu cdng hudng cua carbon ttong do cd 6 carbon bdc IV, 8 carbon b|ic III, 1 carbon bac II va khdng cd tin hieu ciia carbon bac I. Trong sd dd cdc carbon bac IV co do dich chuySn hda hpc 5c tai 193,5 ppm;

166,7 ppm; 165,5 ppm; 159,0 ppm d vimg tiidng thdp dac tnmg cho cdc carbon lai hda sp va cd gdn di td cd dp am dien ldn thdng thudng la oxy. Carbon bac IV cd 6c tai 193,5 ppm Id nhdm C = 0 ciia vdng C. Cdn lgi 2 carbon bdc IV vdi dp dich chuyin hda hpc Sc 131,1 ppm; 115,0 ppm la cdc carbon bac IV mang ndi doi cua vdng thom. Trong sd cac 382

TAP CHI Y Dl/Qt Hpc CAN THg - SO 11-12/2018

carbon bac III cd 2 tin hieu kep (# 4 CH) d 5c 129,0 ppm va 116,3 ppm, chiing td cd su ddi xiing, su ddi xiing chi cd thS tten vdng B, 4 CH ddi xiing nay thudc vdng B (2/6 vd 3/5), chiing td vdng B cd nhdm thi tiidng thudng la hydroxy. Hai proton doi xiing 5H 7,34 ppm va 6,84 ppm cd tiiong tdc HMBC vdi 5c 159,0 ppm, nhdm thS -OH gdn vao carbon nay (C-4).

Hai carbon bac IV 5c 166,7 ppm vd 165,5 ppm cd gdn vdi di td co dp am dien ldn thdng thudng la hydroxy. Cac carbon bac m , 1 carbon bgc III cd 5c 129,8 ppm, gia tti 5H la 7,75 ppm la mdt dinh ddi (d) cd tuong tac vdi hdng sd ghep 7 = 8,5 Hz diiu nay cho tiiay proton nay tuang tac ortiio vdi mdt proton khac, 1 carbon b$c III cd 5c 103,8 ppm vdi gid tr; 5H 6,37 ppm la mdt dmh ddi (d) cd tirong tdc vdi hdng sd ghSp J - 2,5 Hz, diSu nay cho tiiay proton nay cd tirong tac meta vdi mot proton khac, 1 carbon bac III cd 5c 111,7 ppm cd gia tri 5H 6,52 ppm la hai dinh ddi (dd) cd tuong tdc vdi hdng sd ghep J ; = 8,5 Hz va J2 = 2,0 Hz, chimg td proton ndy cd tuong tac ortiio vdng thom vdi proton 5H Id 7,75 ppm va tuong tdc meta vdng thom vdi proton 6,37 ppm. Carbon bac III cdn lai cd 5c 81,0 ppm 5H 5,40 ppm, cd gan vdi nhdm thi cd dp am dien ldn thdng thudng Id hydroxy.

Carbon bdc II cd 5c 45,0 ppm, 5H 3,06 ppm cd tuong tac HMBC qua 3 cau ndi voi C=0, proton nay la H-P va tuong tac COSY vdi proton carbon bac III 5H 5,40 ppm proton nay la H-a.

c a c dii lieu phd cua hgp chat CN25-3 dugc so sanh vdi dii li^u phd cua cac dan chat chalcon da dugc cdng bd trudc dd [6] va cdu tnic hgp chdt CN25-3 dugc xdc dinh la a, 4, T, 4'-tetrahydroxydihydrochalcon.

Hffp chdt CN2S-4

Tinh till hinh manh mau ttdng. ESI-MS m/z: 429,1 [M+H]""; 393,0 [M+H-2H2O].

'H-NMR (CDCI3, 500 MHz) 5H: 4,15 (IH, m, H-1), 4,21(1H, m, H-1), 3,93 (IH, m, H-2), 3,60 (IH, m, H-3), 3,69 (IH, m, H-3), 2,35 (2H, t, J=8,0 Hz, H-a), 1,27 (2H, m, H-3'), 1,25 (br, s, I7CH2), 1,63 (2H, t, J=7,5 Hz, H-4' ), 1,29 (2H, m, H-2'), 0,88 (3H, t, J=7,5 Hz, H-1'), '^C-NMR (CDCI3,125 MHz) 6c: 174,3 (C=0), 65,2 (C-1), 70,2 (C-3), 63,3 (C- 3), 34,2 (C-a), 31,9 (C-3'), 29,6 (I7CH2), 24,9 (C-4'), 22,7 (C-2'), 14,1 (C-1').

Phd khdi ESI-MS cua hgp chdt CN25-4 cho pic ion phdn tii d m/z: 429,1 [M+H]*, 393,0 [M+H-2H2O] cho phep ggi y cdng thirc phan tii la: C26H52O4 cd M = 428.

Phd C-NMR cua hgp chdt CN25-4 cho thdy tin hieu 1 carbon bac IV, 1 carbon bac III, 23 carbon bac 11, 1 carbon b$c I. Carbon bac IV cd dp dich chuySn hda hpc 5c tai 174,3 ppm la nhdm C = 0 . Carbon bac III cd 5c 70,2 ppm la carbon C-2 cua glycerol.

Carbon b a c l cd 5c 14,1 ppm la carbon ddu mach cua acid beo. Trong sd cac carbon bac II, carbon cd 6c 63,3 ppm va 5c 65,2 ppm la cua glycerol, cac carbon bac II cd 5c 34,2 ppm, 31,9 ppm, 24,9 ppm, 22,7 ppm la carbon cua day acid bSo.

Pho HMBC cua CN25-4 cho thdy carbon 6c 65,2 ppm cd hai proton 5H4,21ppm va 8H 4,15 ppm dlu tuong tac HMBC qua 3 day n6i vdi nhdm C=0, nSn carbon la C-l cua glycerol tao Uen kit ester vdi acid b^o. Carbon b|ic II 5c 63,3 ppm cd hai proton 6H 3,69 ppm va 6H 3,60 ppm dlu tuong tac COSY vdi proton 5H 3,93 ppm C-2 ciia glycerol, nen carbon 5c 63,3 ppm la C-3. Carbon bac II cua day acid beo cd 5c 34,2 ppm, 6H 2,35 ppm tuong tac HMBC vdi C = 0 nSn la carbon gdn voi C = 0 . Carbon bac I cd 6c 14,1 ppm la carbon ddu mach cua acid bSo (C-1') cd 5H 0,88 ppm tuong tac COSY vdi proton 5H 1,29 ppm ciia 5c 22,7 ppm la carbon Id C-2' gdn true tiSp vdi C-1', 5H 0,88 ppm cua C-1' tuong tac HMBC qua 3 day n^i vai carbon bac II cd 6c 31,9 ppm, nen carbon ndy Id C-3', carbon bdc II cd 5c 24,9 ppm Id C-4'.

383,

TAP CHf Y DirpC HQC CAN THg - SO 11-12/2018

Cac do lieu pho cua hop chat CN25-4 duac so sanh yod dft lieu ph6 cua cac din chat monoglycerid da dugc cong bo [7] va cau tnic hgfp chat CN25-4 dugrc xac dinh la glycerin 1-monotricosanoat.

Hap chat CN6-2

Tinh thS hinh kim manh, mau tring. 'H-NMR (CDCI3, 500 MHz) 6H: 1,85, 1,08 (2H, m, H-1), 1,83, 1,51 (2H, m, H-2), 3,52 (IH, m, H-3), 2,29, 2,28 (2H, m, H-4), 5,35 (IH, m, H-6), 1,98, 1,48 (2H, m, H-7), 1,47 (IH, m, H-8), 0,93 (IH, m, H-9), 1,50 (2H, m, H-11), 2,00, 1,16 (2H, m, H-12), 1,03 (IH, m, H-14), 1,55,1,03 (2H, m, H-15), 1,70,1,28 (2H, m, H-16), 1,16 (IH, m, H-17), 0,70 (3H, s, H-18), 1,01 (3H, s, H-19), 2,01 (IH, m, H-20), 1,03 (3H, s, H-21), 5,16 (IH, dd, J=15,0, 8,5 Hz, H-22), 5,02 (IH, dd, J=15,0, 8,5 Hz, H-23), 1,53 (IH, m, H-24), 1,53 (IH, m, H-25), 0,92 (3H, d, J=5,5 Hz, H-26), 0,81 (3H, s, H-27), 1,54, 1,20 (2H, m, H-28), 0,84 (3H, m, H-29); "C-NMR (CDCI3, 125MHz) 5c: 37,3 (C-1), 31,7 (C-2), 71,8 (C-3), 42,3 (C-4), 140,8 (C-5), 121,7 (C-6), 31,9 (C-7), 31,9 (C-8), 50,2 (C-9), 36,5 (C-10), 21,1 (C-11), 39,7 (C-12), 42,3 (C-13), 56,9 (C- 14), 24,4 (C-15), 28,9 (C-16), 56,0 (C-17), 12,1 (C-18), 19,4 (C-19), 40,5 (C-20), 21,1 (C- 21), 138,3 (C-22), 129,3 (C-23), 51,3 (C-24), 31,9 (C-25), 21,2 (C-26), 18,9 (C-27), 25,4 (C-28), 12,2 (C-29).

Ph6 C-NMR va DEPT, chat CN6.2 co 29 tin hieu carbon cpng huong, ttong co bao gom 3 tin hieu carbon b$c IV, 11 tin hi|u carbon bac III, 9 tin hieu carbon b^c II, 6 tin hieu carbon bac I cho thay CN6.2 co cSu true dac tnmg cua mot Idiimg sterol.

Tin hieu cua carbon tai vi tri 6c I40,8ppm cho thay day la carbon lai hoa sp^ hon n&a day la carbon bac IV, vay day chi co the la carbon mang n6i doi tr8n vong (C-5). Ba tin hi|u carbon bac III tai vi tri 5c 138,3; 129,3; 121,7ppm, day ciing la 3 carbon lai h6a sp^ trSn ph6 HMBC cho thay proton gan voi carbon a vi tri 5c 138,3ppm tuong tac voi carbon a vi tri 129,3ppm va ngugc 1^, chting to 2 carbon nay da lien ket true tiep voi nhau, vay day la 2 carbon nam tren nhanh (C-22, C-23), ben canh do tir du- USu pho H ya HSQC con chi ra rang 2 proton tren 2 carbon nay nam 6 vi tri trans v6i nhau voi 5H 13n lirgl la 5,16ppm (IH; rfrf; 15; 8,5Hz) \k 5,02ppm (IH; rfrf; 15; 8,5Hz); con Isii carbon tai vi tri 5C 12I,7ppm la carbon nam trSn vong (C-6). Tin hi?u carbon tai vi tri 5c 71,8ppm nSm trong vung truong thdp, cho thdy carbon nay da gin voi di t6 c6 dg Sm di^n cao la oxy, vay day la carbon gSn vdi nhom p-hydroxy (C-3). B6n tin hieu carbon co 5c < 20ppm va 2 tin hi$u 6c 21,2ppm va 21,lppm la cua 6 carbon b^c I. Cdc tin hieu carbon bac II co 8c nam trong khoang (21,lppm - 3I,9ppm) nam trong vimg truang tuong doi thap hon carbon bac I, con lai cac tin hieu carbon b^c III co 5c > 31,9ppm.

Tu do lieu ph6 ID, 2D -NMR, kk hgp so sanh vdi tai lieu tham khao [3] co thS khing dinh CN6.2 la stigmasterol tmh sach.

Theo nghiSn cim cua Awad va Fink (2000), stigmasterol la m6t phytosterol dugc su dung trong phdng ngOa ung thu bu6ng tning, ung thu tuy^n ti^n liet, tmg thu vu va ung thu ket trang.

384

TAP CHIY DlfOC HOC CAN THOf - SO 11-12/2018

CN 25-2 isoliquiritigenin CN25-3 a,4,2',4'-tetrahydroxydihydroclialcon

^CHj

CN25-4 glycerin 1 -monotricosanoat

CN6-2 Stigmasterol

Hinh 1. Cdng thuc cdu tgo cdc chdt phdn l^ dugc IV. K E T L U ^

Tir 300 g cao cdn 96% cua than bim bjp Clinacanthus nutans (Burm. f.) Lmdau tach phan doan bang ky thudt phan bd Idng - long, thu dupe 56,68 g dichloromethan va 15,18 g cao ethyl aeetat. Cao dichloromethan triSn khai qua cot sac ky thu dupe 34 phan doan. TiSn bdnh sac ky cot cd fiSn phan doan 6 va phan doan 25 thu duac 4 chdt tinh khiSt. Cau tnic cac chat dupe xac dinh la Isoliquiritigenin (CN25-2), a,4,2',4'- tetrahydroxydihydrochalcon (CN25-3), Glycerin 1-monotricosanoat (CN25-4) va Stigmasterol (CN6-2).

TAI L I E U T H A M K H A O

1. Vo Van Chi va Trdn Hop (1999), Cdy cd cd ich d Viet Nam tap 1, NXB Giao due tr. 212- 213.

2. AruUappan et al (2014), "In vitro screening of cytotoxic, antimicrobial and antioxidant activities of Clinacanthus nutans (Acanthaceae) leaf extracts", Trop. J. Pharm. Res., 13(9), 1455.

3. Damrong Kongduang, et al. (2008), "Biosynthesis of p-sitosterol and stigmasterol proceeds exclusively via the mevalonate pathway in cell suspension cultures of Croton stellatopilosus", Tetrahedron Letters, 49,4067-4072.

4. Siat Yee Fong (2015), "Genetic, phytochemical and bioactivity studies of Clinacanthus nutans (Burm. f) Lindau (Acanthaceae)", A thesis submitted in fidfilment of the requirements for the degree of Doctor of Philosophy (Applied Biology and Biotechnology), Rmit University.

5. Yahaya R-, et al (2015), "Clinacanthus nutans (Burm.f.) Lindau: An Usefiil Medicinal Plant of South-East Asia", International ofPharnutco^osy and Phytochemical Research, 7(6), 1244-1250.

385

TiflLP CHI Y Dl/Qt HQC CAN THg - s 6 11-12/2018

Ma Q., et al. (2016), "A new isoflavanone from the tmnk of Horsfieldia pandurifolid", Natural Product Research, 30(2), 131-137.

Hemandez-Galicia E, et ai (2007), "Monogiycerides and fatty acids from Ibervillea sonorae root: isolation and hypoglycemic activity", Planta Med, 73(3), 236-240.

Atif B. Awad and Carol S. Fink (2000), "Phytosterols as Anticancer Dietary Components:

Evidence and Mechanism of Action", The Journal of Nutiition, 130:2127-2130.

(Ngdynhdnbdi: 10/11/2017-Ngdy duyet dang: 02/01/2018)

NGHIEN CifV M O HINH B E N H TAT T A I T R A M Y TE XA CUA T i N H CA M A U N A M 2014

Phgm Thj Tam'*, Li Minh Hiru', Hupnh QuSc Vi^t^, Hupnh Kim HSn/

1. Trudng Dgi Hgc Y Dugc Can Tha 2.SdYtiCaMau

*Email:pttam@ctump edu.vn TOM TAT

Dgt van di: Diiu lain kinh ti xd hdi vd Idi sdng cua ngirdi ddn cd dnh hudng mgnh me den md hinh binh tdt [IJ. Dd cd mgt sd nghiin ciht md hinh binh tdt tgi ede binh viin. nhung nghiin euu md hinh b^nh tgt tgi cgng dong cdn khd it, nin chira cung cap dugc cdc bang chiing ddy dii ve nhu cdu chdm sde sue khde eua nhdn ddn. Muc tiiu nghiin cu-u: Xdc dinh ti li cdc chuang benh vd binh mac cao nhdt theo ICDIO eua ede b?nh nhdn khdm chira benh tgi cdc trgmy te xa phudng tinh Cd Mau, ndm 2014. Doi tugng vd phuang phdp nghiin cira: Nghiin euu duac tiin hdnh bdng phuang phdp hdi evru dir liiu ho sa benh dn luu tiii tren mdy tinh cua 130813 ngudi benh din khdm vd diiu trf tu ngdy 1/1/2014 den 31/12/2014 tgi 8 trgm y ti tinh Cd Mau.

Phdn logi bfnh duac xep theo chuang dua theo each phdn logi binh tdt theo ICD-10 theo bgmdS k^ tu eua to chuc y te the gidi gdm 21 chuang. Ket qua: Cdc chuang b^nh chiim ti le cao nhdt tgi trgm y ti la he hd hdp 59,4%; h^ tudn hodn 13,12%; benh hi tieu hda 10%. Otre em. binh he ho hdp (chuangX) Id chii yiu, vdi ti li 91,1% dtre em dirdi dtudivdvd 89,7% tie em 6-15 tudi O ngudi 16 - 59 tudi cao nhdt la Binh hi hd hdp (chuang X) 55,5%. b^nh hi tiiu hda (XI) 15%.

b^nh hi turn hodn (chuang IX) 8,8%, 0 ngudi tir 60 tudi tra lin, bon chirang benh mdc chiim ti li cao nhdt ldn lugt Id Id benh hi tudn hodn (chuang LX) 32,7%, B$nh h4 hd hdp (chuang 20 28%.

Binh h4 ca xuang khdp (chuang XUl) 11.9% vd binh hi tiiu hda (XI) 11,7%. Kit lugn: Mo hinh binh tgt tgi tram y te thay ddi ed xu hudng gia tdng nhdm b^nh khdng ldy, gidm nhom binh nhiim trimg vd chdn thuang ngg dgc.

Tir khda: ICD-10, ngi tiu, ngogi tru, chuang hinh. md hinh binh tgt ABSTRACT

RESEARCH ON PATTERNS O F DISEASES IN C O M M U N E HEALTH C E N T E R S IN 2014

Pham Thi Tam', Le Minh Huu', Huynh Quoc Viet^, Huynh Kim Hon/

1. Can Tho University of Medicine and Pharmacy 2. Ca Mau Department of Health Background: Socioeconomic conditions and lifestyles of people have a strong influence on disease patterns [If. While there have been a number of disease pattern studies in hospitals, 386