VIETHAM MEDICAL JOURNAL N°2 - JUNE - 2017 gia nao ve ddng maeh hang tren sieu am
Doppler hay chdc nang eae day than kinh tren nghien eiTu dan truyen du'dc bao cao lai de xac nhan thanh cdng eua khau ndl vi phau d cac BN nay. Cac tru'dng hdp ndi ghep du'dng vat tai BV Viet Oitc eiia chiing tdi deu cd thdng bao ve xuat hien chiTc nang eudng du'dng sau khi kham lai mdt thang sau md [6]. (Hinh 2). Cam giae du'dc bao ton d 82%. Hep va dd nieu dao ehi xuat hien d 20%.
Mat da van ed the xay ra nhuhg nd thu'dng d mire nheva ndng [2,3,4,6,7].
IV. KET LU^N
Ghep lai bang vi phau la phau thuat duy nhat da chirng mmh diidc hieu qua trong viee ciiu van tinh hoan, du'dng vat dirt rdi. Mdt t / le mac benh tam than cao du'dc tim thay trong sd nhihig BN edn rat tre nay, va nguyen nhan thu'dng la do BN tif gay ra. Ngay khi tiep nhan BN can chi dinh sdm kham hdi chan chuyen khoa tam than de dn d|nh BN tru'dc va sau khi phau thuat. Han ehe ve tlidl gian thieu mau cue bd la rat quan trpng cho sir thanh edng eiia p h l u thuat. Chifc nang sinh san cd the du'dc duy tri vdi thdi gian thieu mau cue bd chirng 6 gid, va chirc nang ndi tiet du'dc duy tri vdi thdi gian thieu mau cue bd dai hdn.
Neu ndl ghep thanh cdng thi ket qua chu'c nang,
tham my va tam ly se cao hdn rat nhieu so vdi tai tao tiang cac chat lieu tir than hay nhan tao.
Chinh vi vay viec chuyen BN den cac trung tSm Tao hinh Vi phau cd kinh nghlemse giup toi da hda thanh cdng d nhu'ng BN nay.
TAI UEU THAIVl KHAO
1. Becker M, Hofner K, Lassner F, Pallua N, Berger A. Replantation of the complete external genitals.
Plast Reconstr Surg. 1997 Apr; 99(4): 1165-8.
2. Flores RL, Hazen A, Gatiano RD, Klapper AM, Levine JP. Non extremity replantation: the management of amputations of the facial parts and testicle. Clin Plast Surg. 2007 Apr; 34(2): 197-210.
3. Griffiths M, Britto J, Frame J. 5-year follow-up of replantation of penis and testis in a child Lancet. 2003 Jan 18;361(9353):263.
4. Jezior JR, Brady JD, Schlossberg SI«t.
Management of penile amputation injuries. World J Surg 2001 Dec;25(i2): 1602-9. Review.
a. Lin SD, Lai CS, Su PY. Replantation of the testis by microsurgical technique Plast Reconstr Surg 1985;76(4): 620-5.
6. Tran Thj Thanh Huyen,Nguyen Hong Ha. BUdc dau irng dung cac kT thuat tao hinh dieu tri thirdng ton mac phai cO quan sinh due ngoai nam gicii. Y hoc Viet Nam, 2014 thang 12, tr 159-163.
/ . Virasoro R, Tonkin JB, McCammon KA, Jordan GH.Penile Amputation: Cosmebc and Functional Results. Sex I^ed Rev, 2015 Jul;3(3):214-222.
DANH GIA TAC DUNG CHONG VIEM DI ITNG VA LAM BEN TE B A O MAST CIJA CAO DAC EZ TREN THITC NGHIEM
Nguyin Mgnh T u y i n ' , Pham Thi VSn Anh^
T 6 I V I TAT
Mgc tieu: Danh gia tac dung chdng viem di iTng va lam ben te bao mast ciia cao dac EZ tren thUc nghiem. Bdi tu'dng va phu'dng phap: Cao dac bai thuoc dUde bao che tir dich chiet nu'dc va dich chiet ethanol bai thudc EZ dudc danh gia tac dung chdng viem di iing cap tinh va lam ben te bao mast thong qua md hinh thiTc nghiem tren chupt nhat trang bang tac nhan gay viem di utig compound 48/80. Kit qua:
Cao EZ-HjO 6 lieu 3,5g/kg va 10,5 g/kg, cao E2-EtOH lieu l,92g/kg co tac dung co tac dung chong viem di irng thUc nghiem tren chuot nhat trang do compound 48/80 gay ra. Cao E2-H2O d ea 2 lieu thir 3,5g/kg va 10,5 g/kg, cao EZ-EtOH d ca 2 lieu thir l,92g/kg va
' Trddng Dai hoc Dddc Ha Ngi
^Trddng Dai hgc Y Iia Nqi
Chju trach nhiem chinh: Nguyen Manh Tuyen Email: [email protected] Ngay nhan bai: 24.3.2017 Ngay phan bien khoa hoc; 15.5.2017 Ngay duyet bai: 23.5.2017
5,76g/kg d§u khdng the hien tae dung lam ben viJng te bao mast khi ii vdi compound 48/80. KeiluamCao dac EZ cd tac dung chdng viem di ijng va co xu hifdng lam ben te bao mast tren thUc nghiem.
T&khda: cao dac EZ, dimg viem di ifng, te bao maft.
SUMMARY
EFFECTS OF ANTI-ALLERGIC AND SUSTAINABLE MAST ACTIVITIES CELLS OF
VISCOUS EZ ON EXPERIMANT MODELS Objectives: To evaluate effects of anti-allergic and sustainable mast cells activities of EZ viscous extracts. Subjects and methods: EZ viscous extracts, which were made fnam the ethanol 70% and water extracts were evaluated the effect of anti- allergic on foot edema model and the effect on sustainable mast cells models by allergic agent as compound 48/80. Results: The results showed ttiat, the viscous extracts of EZ remedy, which were made from the water and ethanol extracts in dose of 14.4g/kg and ethanol extract of EZ in dose of 43.2 g/kg (raw materials) showed significantly inhibitory effects on inflammatory-edema effects on foot edema
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TAP CHi Y HQC VIET NAM TAP 455 - THANG 6 • SO 2 - 2017 model induced by compound 48/80 (p<0.05). But the
extract had no effect of sustainable mast cells.
Conclusion: EZ viscous extracts (both water and ethanol extracts) may be beneficial in the regulation of immediate-type allergic reaction.
Keyvvords: EZ remedy viscous extract, anti- allergic, inflammatory, sustainable mast cells, I. OAT VAN €)E
Bai thudc EZ du'dc xay du'ng du^ tren cd sd ly luan y hoc co truyen ket hdp vdl cae ket qua nghien ciiu thiic nghiem vdi thanh phan ehii du'dc la cac vj thudc Kim ngan dang, Niie nac, Hoe hoa... nham mue dieh dieu tn eae trieu chii'ng benh viem da cd dia. Cae nghien eiru da cong bd cho thay: eao ehiet ethanol va eao chiet nu'dc EZ cd tac dung khang viem cap va man tinh'^^'^^^ Nghien ciru nay du'dc tiep tuc thi/c hien nham mue tieu danh gia tae dung ehdng di iTng ciia eao EZ chiet ethanol va ehiet nu'dc tren thy'c nghiem thdng qua tac dung chong viem di irng va t^c dung lam ben viTng te bao mast tren chuot nhat trang.
II. £>dl TU'ONG VA PHU'OTMG PHAP NGHIEN CUXJ 1 . Ddl tu'dng nghien cu'u:
Bai thuoc EZ gdm: kim ngan dang i^Caulis cum folium Lonic^rae), niic nae {Cortex Orvxyli), hoe hoa {Fios Styphnobbiijaponiciimaturi), thu'dng nhT tir {Fructus Xanthii strumarii), hoang ba {Cortex Phellodendri) dat tieu ehuan DDVN IV.
Cao dac d&dc bao che t& dich chiet ethanol bai thudc EZ (ggi tat la cao EZ- EtOH): can rieng tirng du'cfe lieu da du'dc xi^ ly dat tieu chuan theo khdi lu'dng tu'dng irng vdi 5 thang (300g du'dc lieu). Chiet hdi lu'u rieng tirng vi bang ethanol 70% 3 ian, moi lan 2h vdl lu'dng ethanol gap 5 lan du'dc lieu. Phoi hdp djch chiet, cat thu hoi dung mdi, ly tam lay dich. Cd dac tren each thiiy thu du'dc 40g cao dae, hay l g eao dae tu'dng du'dng 7,5g du'dc lieu.
Cao dac d&dc bao chet& dich chiit n&dc bai thudc EZ'(ggi tat la cao EZ-H2O): can rieng tirng du'dc lieu da du'dc xii ly dat tieu chuan theo khdi lu'dng tu'dng irng vdi 5 thang (300g du'dc lieu). Chiet xuat bang nu'dc theo phu'dng phap sac, dun sdi 3 lan, moi lan I h , lu'dng nu'dc gap 6 lan du'dc lieu. Loc ndng qua vai gac, ly tam loai tap chat Id lirng va gdp dich chiet.' Cd dae thu du'dc 73,8g eao dae, hay l g eao dae tu'dng ifng vdi 4 , l g dUdc lieu.
2. b o n g vat nghien ciru: Chuot nhat trang chiing Swiss, thuan chiing, ca hai^ gidng, nang 25+2 gram do Vien Ve sinh dich te trung u'dng cung cap. Ddng vat du'dc nudi trong dieu kien phdng thi nghiem Bd mdn Du'dc ly, Tru'dng dai
hoc Y Ha Ndi tir 5- 7 ngay tru'dc khi nghien cii'u va trong sudt thdi gian nghien ciru.
3. Phu'dng phap nghien cu'u
3.1. Nghiin c&u tac dung chdng di &ng bang md hinh gay viim cap tinh trin chan chuot nhat trang: 70 ehudt nhat trang du'dc chia ngau nhien lam 7 Id, mdi Id 10 con.
Ld 1 (Chirng sinh ho(^: cho udng nu'dc cat vdi the tieh tu'dng du'dng mau thir;
Ld 2 (Md hinh): cho udng nu'dc cat vdi the tieh tu'dng du'dng mau thir;
Ld 3 (Chirng du'dng 1): eho udng methylprednisolon 6 mg/kg can nang chupt (mg/kg);
Ld 4 (Cao EZ-H2O lieu thap): eho udng cao EZ- H2O lieu 3,5g/kg (tudng img vdi 14,4g Du'dc lieu/kg);
Ld 5 (Cao EZ-H2O lieu cao): cho udng cao EZ-H2O lieu 10,5g/kg (tu'dng img vdi 43,2g Du'dc lieu/kg);
Ld 6 (Cao EZ-EtOH lieu thap): cho udng cao EZ-EtOH lieu l,92g/kg (tu'dng irng vdi 14,4g Dddc lieu/kg);
Ld 7 (Cao EZ-EtOH lieu cao): cho udng cao EZ-EtOH lieu 5,76g/kg (tu'dng ifhg vdi 43,2g Diidc lieu/kg).
Cae mau thir du'dc pha den ndng dp thich hdp de moi lan cho thude tu'dng irng vdi b,2mL/l6g can nang ehudt. Bong vat thi nghiem du'dc eho udng nu'dc cat, m l u chiTng du'dng hoac thude thir vdi lieu nhu' tren trong 5 ngay lien tiep. Cen ngay thir 5, sau khi eho udng mau trang, thuoc ehij'ng, mau thir 1 gid, ngoai trir Id chu'ng sinh hoc dUde tiem 0,02mL nu'dc mudi smh ly, cac Id cdn lai du'dc tiem 0,02mL dung dieh compound 48/80 0,4% (pha trong nu'dc mudi sinh ly) 0,02mL du'di da gan ban chan phai.
Chi sd nghien e&u.
- Chieu day chan ben phai ehudt du'dc do tru;dc va sau khi tiem compound 48/80 tai thdi diem 30, 60 phiit d tat ca cac Id chupt.
- Od gia tang the tich chan chupt: The tich ehan ben phai ciia chupt dddc do tru'dc va sau khi tiem compound 48/80 tai thdi diem 30, 60 phiit d tat ca eac Id ehudt de xae dinh dd gia tang the tich d 2 thdi diem 30, 60 phiit so vdi ban dau. So sanh gia tri trung binh ve mdc dp gia tang the tich giiTa eae Id de danh gia tac dung chdng dj irng.
3.2. Banh gia v&ng b'en tebao mast: Thi nghiem dUdc bd tri tu'dng tu' nhu' mue 2.4.1. vdi 70 eh^udt nhat trang du'dc chia ngau nhien lam 7 Id, mdi Id 10 con. Thiet ke cae Id chu'ng sinh hpc, chirng du'dng va thir vdi lieu thir tu'dng tii. Oen ngay thir 5, sau khi eho udng thudc thir, moi ehudt du'dc tiem mang bung 1,5ml nddc muoi sinh ly. Sau do mo chuot, thu djch 6 bung, ly tam de thu thap te bao mast, rdi ii vdi dung dich compound 48/80 ndng dd Ipg/mL trong 10 phiit.
201
VIETNAM MEDICAL JOURNAL N°2 - JUNE - 2017 Rieng Id 1 khdng u vdi compound 48/80. Sau do,
te bao mast du'dc nhudm xanh toluidine 0,1%, dem sd te bao mast khdng bj giang hda. So sanh ty le phan tram \s bao mast khdng bi giang hda giuS cae Id de danh gia tae dung bao ve ie bao mast.
4. Xur ly so lieu: Cae sd lieu thu thap du'dc xu" ly bang phu'dng phap thdng ke y sinh hoc theo TJest - Student. Ket qua du'dc trinh biy dang: x ± SE. Su" khae biet ed y nghTa thdng ke khi gia tri p < 0,05.
III. KET QUA NGHIEN CU'U
1 . Tac dung chong viem d| i^ng cua cao dac EZ
Bang 1. Anh h&dng aia cao dac EZ ten the b'ch chan chugt (n=10) Lo
Lo 1 fChLfna sinh hoc) Lo 2 (Mo hinh)
Lo 3 (l^ethyl- prednislon, 6mq/lcq) Lo 4 (EZ-H,0, 3,5q/lcq:
Lo 5 (EZ-H2O, 10,5g/kq) Lo 6 (EZ-EtOH,
1,92 q/l<q) Lo 7 (EZ-EtOH,
5,76q/kq)
Chieu day chaachuot tai cac thdi diem ( X ± SE, mm) Ophiit
1,57 ± 0,02 1,55 ± 0,03 1,50 ± 0,02 1,51 ± 0,02 1,51 ± 0,02 1,52 ± 0,02 1,50 ± 0,03
30 phut 2,09 ± 0,05 3,67 ± 0,10
3,85 ± 0,08 3,78 ± 0,08 3,79 ± 0,11 3,65 ± 0,10 3,89 ± 0,10
60 phut 1,73 ± 0,05 3,32 ± 0,08
3,35 ± 0,08 3,48 ± 0,12 3,56 ± 0,03 3,45 ± 0,10 3,68 ± 0,08
Gia tang the tich chSn ehudt tai cac thdi diem ( X ± SE,o/ol
30 phut 10,21 ± 2,63 67,89 ± 7,33' 46,08 ± 3,40' 43,75 ± 4 , 0 2 "
41,22 ± 3,89"
41,42 ± 6,58' 56,40 ± 7,75
60 phut 5,14 ± 1,61 52,48 ± 8,23"
42,80 ± 4,54 42,55 ± 6,42 37,72 ± 3,46 24,12 ± 6,17*''
40,32 ± 7,39 Ghi chu: so vdi Id 2 (md hinh): : p<0,05;
p<0,01; so vdi Id 3 (chiTng du'dng Methyl- prednisolon, 6mg/kg), ": p<0,05. Compound 48/80 gay phii ehan ehudt rd rang so vdi Id ehimg sinh hpe ehi tiem nu'dc mudi sinh ly (p<0,01), chirng td md hinh gay viem bang compound 48/80 thu'c sir thanh cdng. Methylprednisolon 6mg/kg cd tae dung lam giam sir gia tang the tich chan chupt cd khac biet_cd y nghTa thdng ke so vdi Id md hinh.
' 6 Id 4 va 5, cao EZ-H2O d lieu 3,5g/kg va 10,5 g/kg deu the hien tae dyng ii'e che gia tang the tich chan ehudt d thdi diem 30 phiit (p<0,01), nhu'ng khong lam giam ehieu day ehan chuot so vdi Id md hinh.
a Id 6, cao EZ-EtOH, lieu l,92g/kg cd tac dung ire che gia tang the tich ehan chupt d thdi diem 30 phiit (p<0,05) va thdi diem 60 phiit (p<0,05) nhu'ng khdng lam giam chieu day chan ehudt so vdl Id md hinh. 6 thdi diem 60 phut, eao EZ-EtOH, lieu l,92g/kg cdn ed tae dung ii'e che gia tang the tich etian chupt manh hdn so vdl thude chiTng du'dng methyl-prednisoion, lieu 6mg/kg (p<0,05).
O Id 7, cao EZ-EtOH, lieu 5,76g/kg khdng co tac dung giam kich thu'dc chan ehudt va giam gia tang the tieh ehan ehudt so vdl Id md hinh cd y nghTa thong ke.
2. Tac dung iam vij'ng ben te bao mast ciia cao EZ Bang 2. Anh hu'dng cua cao EZ ien tv ie vd cua tebao mast
Lo L6 1: Chu'nq sinh hoc Lo 2: Mo hinh compound 48/80 Lo 3: Methylprednision (6mq/kq)
Lo 4: EZ-H2O (3,5q/kq) Lo 5: EZ-H2O (10,5q/kq) L06: EZ-EtOH (1,92 q/kq) Lo 7: EZ-EtOH (5,76 q/kq)
n 10 10 10 10 10 10 10
Ty ie t e bao mast v3 ( x ± SE, % ) 3,0 ± 1,3 14,5 ± 2,7 ( P I - . < 0 , 0 1 )
8,5 ± 2,1 14,5 ± 1,9 13,0 ± 1,7 10,5 ± 1,6 14,0 ± 3,8 O Id md hinh, compound 48/80 gay v3 te bao
mast vdi ty le 14,5%, khac biet ro ret so vdi nhdm chu'ng sinh hpc vdi p < 0,01.
Thuoc chutig du'dng methylprednisolon, lieu 6 mg/kg cd xu hu'dng giam ty le v3 te bao mast tuy nhien sir khac biet so vdi Id md hinh khdng cd y nghTa thdng ke (p>0,05).
Cac mau thir cao EZ-HjO va EZ-EtOH d ca hai
lieu thu' deu khdng lam giam ty le vd te bao mast ed y nghTa thong ke so vd^ Id md hinh (p>0,05).
IV. BAN LUAN
Compound 48/80 la san pham polymer hda eua n-methyl-p-methoxy-phenethylamlne vdl formaldehyde tir 3-6 ddn phan ed vai trd la mpt di nguyen gay kfch iTng gial phdng histamin titt?
TAP CHl Y HOC VIET HAM TAP 455 - THAHG 6 - SO 2 - 2017 bao mast, tir dd kieh thich te bao mast giai
phdng tiep cae ehat trung gian gay ra eae bieu hien dj iTng. Compound 48/80 khi sir dung vdl lieu khae nhau va du'dng dimg khae nhau gay ra cac bieu hien dj img eung khac nhau. Khi dimg lieu eao tiem tru'c tiep ed the gay sdc phan ve, trong khi diing lieu thap tiem du'di da ehi gay ra cac tinh trang phu ngira, do do compound 48/80 la chat thirdng du'dc dung trong cae md hinh dj umg^^J Hien tu'dng viem dan tdi phii ia mpt hien tu'dng hay gap d dj iTng, nguyen nhan chu yeu la do gian maeh thoat dieh, day la ed ehe gay viem cap tren ehan ehudt nhat trang.
Ket qua nghien citu eung eho thay eao EZ-H^O d ca 2 lieu thir 3,5g/kg (tu'dng uYig vdi 14,4g du'dc lieu/kg) va 10,5g/kg (tu'dng iTng vdl 43,2g du'dc liiu/kg), cao EZ-EtOH d lieu l,92g/kg (tu'dng irng vdi 14,4g du'dc lieu/kg) cd tac dung ire ehe viem di irng trong md hinh gay phii ehan ehudt.
Cac tac gia Han Qude (2008) cho rang, djch chiet niTdc cua kim ngan dang ed tae dung len thu the PAR2 (proteinase-aetivated receptor 2) gay phii d ehudt d lieu 50, 100 va 200mg/kg eho tae dung ije ehe phii chan ehudt ro ret (iic ehe thay ddi chieu day va tinh tham maeh mau)^''l Rutin va quercetin trong hoa hoe eung ed tae dung iic che phii ban chan ehudt do histamin gay nen.
Theo cd ehe dj iTng hien tu'dng vd te bao mast la hien tu'dng trung gian de glai phdng eac yeu t d trung gian gay viem. Compound 48/80 ed tac dung lam ly gial te bao mast, do do khi ii te bao mast vdi compound 48/80 se xac dinh du'dc kha nang ben vij'ng ciia te bao mast. Cao EZ ehiet nu'dc va con d ca 2 lieu thOr cd xu hu'dng lam giam ty le te bao mast khi u vdl compound 48/80, tuy nhien t i e dung chu'a rd ret (p>0,05).
Do vay cac nghien ciTu tir gde dp nay can du'dc
tiep tuc tien hanh de tim hieu them cd che tae dung eiia bai thudc EZ.
V. KET LUAN
Cao E2-H2O d lieu 3,5g/kg va 10,5 g/kg, cao EZ-EtOH lieu l,92g/kg cd tae dung cd tac dung chdng viem cap thy'c nghiem tren chupt nhat trang do compound 48/80 gay ra. Cao EZ-EtOH lieu 5,76g/kg khdng cd tae dung nay.
Cao EZ-H2O d ea 2 lieu thir 3,5g/kg va 10,5 g/kg, eao EZ-EtOH d ea 2 lieu thir l,92g/kg va 5,76g/kg deu khdng ed tac dung lam ben vu'ng te bao mast khi u vdi compound 48/80.
TAI UEU T H A M KHAO
1. Nguyen Manh Tuyen va cs (2015), Danh gia tac dung chong viem thUc nghiem ciia cao dae bao che tir dich chiet ethanol bai thudc EZ, Tap chi Dddc hoc, so 7, tr6-10.
z. Nguyen Manh Tuyen va cs (2015), Danh gia tac dung chong viem thyc nghiem ciia cao dac bao che tiT dich chiet rnrdc bai thudc EZ, Tap chi Dudc hoc, so 10, tr 6-10.
3. Chandrashekhar V M l , Haiagali KS, Nidavani RB (2011), Anti-allergic activity of German chamomile {Matricaria recutita L.) in mast cell mediated allergy model, J Ethnopharmacol 137(1) 336-40.
4. H. R. Chitme, Mallikarjun Malipatil, V. M.
Chandrashekhar and P. M. Prashant (2010), Antiallergic activity of Arlstolochi biacteolata Lank in animal model, Indian Journal of Experimental Biology, Vol. 48, pp. 46-52.
s. Jin-Mu Y et al (2000), Ixons dentata Green Sap Inhibits Both Compound 48/80-Induced Aanaphylaxis- ijke Response and IgE-Mediated Anaphylacbc Response in Munne Model, Bic^. Phann. Bull. 25 (1); 5-9.
6. Wedemeyer J, Tsai M, GIN S J (2000), Roles of mast cells and basephiis in innate and accquire immunity, Curr Opin Immunol 12(6) 624-31.
7. Yoo HJ, Kang H3, Song YS, Park EH, Lim CI (2008), "Anti-angiogenic, anti-nociceptive and anti-inflammatory activities of Lonlcera japonica extract, J Pharm Pharmacol, 60 (6): 779-86.
THU'C TRANG NHIEM K H U A N BENH VIEN TAI BENH VIEN S A N NHI NINH BINH N A M 2 0 1 5 - 2 0 1 6
TOM TAT
Bang nghien ciru md ta cat ngang cd phan tich 'Benh vi0n San Nhi Ninh Binh
^IJen hiep cac hoi Khoa hgc va ky thuat tinh Ninh Binh Chju trach nhiern chinh: Do Van Dung
Email: [email protected] Ngay nhan bai; 22.3.2017 Ngay phan bien khoa hpc; 15.5.2017 Ngay duyet bai: 24.5.2017
Bui Thi HiTffng', Do Van Dung^
tren 1450 benh nhan co thdi gian nam vien tir 48 gid trd len tai Benh vien Sim Nhi Ninh Binh trong nam 2015-201*6, ket qua cho thay:
- Ty le hien mac nhiem khuan benh viSn tai Benh vien San Nhi Nmh Binh la 7,17%. Trong do cao nhat la nhiem khuan ho hap vdi 56,73%; tiep den la nhiem khuan tieu hoa vdi 17,31%; nhiem khuan vet mo la 15,38%; nhilm khuan da, mo la 6,73%, thap nhat la nhiem khuan huyet vdi 3,85%.
- Tac nhan gay nhiem khuan benh vien:
Pseudomonas aeruginosa, tu cau vang va Klebsiella pneumonia la nhCTng chiing vi khuan hang dau gay
