VIETNAM MEDICAL JOURNAL N°1 - JULY- 2017 Trong nghien eiTu mdi nhat nam 2015 tac^gia
Mohammad cung nhan manh TB ap va mo benh hpe manh sinh thiet phai du'dc tien hanh dong thdi trong tru'dng hdp npi soi co ket qua nghi ngd ung thd.
V. KET LUAN
Dac diem hinh anh ndi soi da day - Vi tri ton thu'dng hay gap la hang vi (60,5%), va bd cong nho (14,6%). ^
- Ton thu'dng npi soi chii yeu the loet va loet sui (31,2% va38,4%)._
- Npi soi da day chan doan diing dddc 86,9%
so benh nhan UTDD.
Dac diem ket qua x e t nghiem t e bao ap va mo benh hoc
- Te bao ap la phu'dng phap chan doan ddn gian, thdi gian chan doan nhanh, gia thanh re va CO sd phii hdp 98,6% so vdi mo benh hoe.
- Da so the mo benh hpc eua UTDD la ung thd bleu mo tuyen da day chiem 92,7%.
TAI LIEU T H A M KHAO
1. Globocan (2012). Cancer Incidence and Mortality Worldwide: lARC CaneerBase No. 11, Intemadonal Agenqy tor Research on Cancer.
2. Nguyen Thj VUdng (2013). Danh gia ket qua hoa tri lieu pha ao Xelox trong dieu tn bo tra ung thu'dc day Luan an thac si y khoa tru'dng Dai hoc Y Hn.
3. Trjnh Hong Sdn (2001), Ughlen cdu nao vet hach trong dieu tn phauthuat ung thu" da day, Luan ,an Tien sy y hoc, Trudng Oai hoe y Ha Noi.
4. Bui Anh Tuyet (2003), Nhan xet dac dim iam sang, hinh anh noi soi va nid benh hoc cua ung thu' da day di'eu tn tai Benh Vien k tir thang 9/2002- 6/2003. Luan an Thac sy y khoa, TrUcfng OaiH(3eYHaN6i.
5. Trjnh Quae Hoan(2001). Nghien cdu hinh anh giai phau benh hoc cua ung thu'da day. Hoi thao ^n 2- trung tam hdp tae nghien culi eiia To chifc y te the giqfi ve ung thu' da day.Benh vien K. Ha npi. 40-50.
6. Do Du'c Van, Trjnh Hong Scfn, Go Tru'dng Stfn: Adenocareinomes ta trang. Ngoai Khoa 1995;
Tap XXV-2: 1-4.
7. Young JA et al (1980). Three year trial of endoscopic cytology of die stomach and duodenum.ffi/f. 1980 Mar;21(3):241-6.
NGHlfeN CU'U DOC TINH CAP VA DOC TINH BAN TRU-ffNG DIEN
COA B O T A C I D A M I N T H U Y PHAN TIT DA CA TRA, CA BASATHEN DONG VAT THU'C NGHIEM
Le Thi Hong HanhMai Thi Minh Nggc**, T O M TAT
Hue tieu: Danh gia du'dc doc tfnh cap va doe tfnh ban tru'dng diln eiia che pham bgt acid amiii thiiy phan tir da ea tra, ea basa . Doi tu'dng va phu'dng phap nghien CLTU: bpt acid amln dddc danh gia doc tinh cap tren 42 chuot nhat trang va thiT doc tfnh ban tru'dng dien trSn 30 chuot cdng trang theo quy dinh chuan. Ket qua: nghien ciTU doe tinh cap ciia hot acid amin tren chuot nhat tring theo du'dng uong: LD50 = 14,17 ± 0,43 (g/kg TLCT/24h). Nghien eiTu doe tinh ban trddng dien, cho chu6t cong uong bot acid amin 42 ngay vdi lieu 140 mg/kg va 280 mg/ TLCT kg/24h.
Khong c6 su" khac biet co ^ nghTa thong ke so vdi 16 chiTng 6 tat ca eac ehi so: trpng lu'dng ctJ the, so luong hong cau, huyet sac to, so lu'dng bach cau, tieu cau, AST, ALT, ure, ereahnin. Hinh anh mo benh hoc cLia gan, than binh thu'dng.
*Hoc vien Quan y
**Vien Dai hoc Md Ha Noi
***Vien Y hoc Hang Khong^
Chju trach nhiem chinh: Do Minh Trung Email: [email protected] Ngay nhan bai: 12.4.2017 Ngay phan bien khoa hoc: 24.5.2017 Ngay duyet bai: 6.6.2017
*, D6 Minh Trung*, Nguyin Thanh Xuan*, Le Thj Huy£n Trang***, Ph?m Thi Tain**
Td khoa: Acid amin. Doc tinh cap. Doc tinh ban tru'dng dien.
SUMMARY
STUDY ON THE ACUTE AND SUBCHRONIC TOXICITY OF AMINO ACID POWDER FROM HYDROLYZED FROM SKIN OF PANGSIIDEA, PANGASIUS ON EXPERIMENTAL ANIMALS Objectives; Amino acid powder was hydnalyzed from skin of Pangsiidea, Pangasius. Materials and Methods: Amino acid powder was used to study on acute toxieitv in 42 mice and subchronic toxicity 30 rats by Standard Operating Procedure. Results: The result of acute toxicity of the amino acid powder: LDM
= 14,17 ± 0,43 (mg/kg/24h). The studying of the subchronic toxicity demonstrated that daily administering of the amino acid powder with doseage of 140 mg/kg and 280 mg/ TLCT kg/24h during 42 to rats, the base parameters have no signification for tiie weight of the body, Hematological indices (red blood cell, white blood cell, platelet and hemoglobin contents), hepatic indices (AST, ALT activity) and renal dicices (urea, creatinin levels) of rats after amino ado treatment were in normal biological values. Tissue images of the liver and the kidney are normal.
Key words: Acid amin. Acute toxicity, Subchronic toxicity.
148
TAP CHi Y HOC V l i T NAM TAP 456 - THANG 7 - SO 1 - 2017 I. OiDkT V A N OE
Gelatine la protein dddc tao ra td qua trinh thiiy phan collagen tiT da Idn, da bo, da ea, xu'dng. Cae acid amin ehii yeu cau tao nen gelatine la glycine, proline, 4-hydroxyproline va aspartie. Trong do acid amin glycine da dddc chiTng minh co tac dung ngan ngiTa sd lao hoa va tao ra do san chac cho da. Proline va 4- hydroxyproline la cae acid amin ly tu'dng cho viec phuc hoi khdp xu'dng, cd, cac mo va da. Axit aspartie rat quan trpng cho sd tang tru'dng mo va cd, tai tao te bao.
Hien nay vdi san lu'dng ca ea tra, ea basa nguyen lieu hang nam nhd hien nay, eac nha may che bien phai thai ra thi truSng hang tram nghin tan phe pham. Lu'dng chat thai nay eo the dude tan dung de die bien thanh thirc an chan nuoi, dau ea, dlesel sinh hoc, hoac xuat khau de lam nguyen lieu cho cong nghiep dude pham, my pham.
_^Viec tan dung phu pham da ea tra, ca ba sa de thiiy phan tao gelatine va cac ehiet xuat acid amin tao nguon nguyen lieu san xuat thu'c pham chiTc nang va my pham khong chi giai quyet du'dc 6 nhiem moi tru'dng ma eon tao ra eac san pham mdi eo nhieu tiem nang iTng dyng. Tuy nhien che pham acid amin tao dddc can du'dc danh gia tinh an toan tru'dc khi nghien ciTu va iTng dung d cac giai doan tiep theo. Chfnh vi vay trong nghien ciTu nay sau khi thuy phan da ca tra, ca basa bang gelatlnase va tao thanh che pham bpt acid amin chiing tdi tien hanh danh gia doc tfnh cap va doe tfnh ban tru'dng dien eiia che pham acid amin nay.
II. o6\ TU'ONG VA PHUONG PHAP NGHIEN CU'U 2 . 1 . Dol tuftfng n g h i e n c i h j
Bdl tddng nghien ciiii: Che pham bpt Acid amin thiiy phan tiT da ca tra, ca basa (co thanh phan - mg/lOOg mau: Glycine 3570 mg; Arginine 3024mg; Thrlonlne 2070mg; Alanine 70mg;
Methionine 3884mg; Lysine 2350mg) la san pham thuoc de tai ma so KC06.15/11-15.
Dpng vat thi nghiem: Chupt nhat trang dong Swiss tru'dng thanh, dii tieu chuan thf nghiem, khoe manh dat tieu chuan thf nghiem, trong
lu'dng 20g - 22g, Dong vat nghien eiTu do ban chan nuoi Hpe vien Quan y cung cap.
2.2. Phu'dng phap n g h i e n cu'u
^•2.1. Xac djnh ddc tinh cap theo du'dng udng: Xac djnh doc ti'nh cap theo du'dng uong dddc thdc hien theo phu'dng phap llvschitz P.G va cpng sd [1],[3].
2.2.2. NghiSn cud ddc tinh ban tru'dng dien cua ehS' pham bdt Acid amin theo du'dng udng tren chupt cdng trang [4],[5]
- Chupt e6ng_^ dddc phan ehia ngau nhien thanh 3 nhom, moi nhdm ed 10 chupt
+ Lo chiTng (n=10): uong nu'dc cat lieu l,5ml/kgthetrpng/24h.
+ Lo diing thuoc lieu 1 (n=10): uong bot acid amin vdi the tfch l,5ml/kg dich thuoc tddng du'dng lieu 140mg/kg the trpng/24h
+ Lo diing thuoc lieu 2 (n=10): uong bot acid amin vdi the tich l,5ml/kg dich thuoc 'tu'dng du'dng 280mg/kg the trpng/24h
+ Chupt cong trang dddc cho uong nu'dc va thuoc thiT lien tuc trong 6 tuan lien tiep, moi ngay mot lan vao buoi sang
- Cic chi tiSu danh gii:
+ Theo doi tinh trang chung, hoat dong, an uong, trpng lu'dng cd the
+ Huyet hpc: hong cau, hemoglobin, bach cau, tieu cau.
+ Sinh hoa: AST, ALT, ure, creatinin.
Mo benh hoc: vao ngay thd 42, giet chupt, lay gan, than, quan sat hinh anh dai the gan, than va lam tieu ban darih gia hinh anh mo benh hpc.
- Thdi diem xet nghiem: Lay mau xet nghiem cae ehi so sinh hoa, liuyet hpc, ghi tai 3 thdi diem: xuat phat diem, sau 3 tuan, sau 6 tuan nghien cu'u. Thdi gian theo doi la 6 tuan,
- Xdly vi phin tieh so lieu: Phan tieh thong ke bang chu'dng trinh chuyen dung Startview.
III. KFT QUA NGHIEN CU'U
3.1, Ket qui nghien cud ddc tinh ci'p cua bpt^acid amin theo du'dng udng tren chupt nhat trang
Ket qua doe tfnh cap cua che pham bpt acid amin tren chupt nhat bang dddc the hien 6 biing 1:
Bang 1. Ket qua nqhien CLTU doc tfnh cap cua che pham bot acid amin theo dirdnq uonq TT
1 2 3 4 5 6 7
Li€u sii* d u n g (q/liq T L a 7 2 4 h )
11,00 12,00 13,00 14,00 15,00 16,00 17,00
n 06 06 06 06 06 06 06
So lu'dng d p n ^ v a t chet'(sau i tuanj
0 01 02 02 04 05 06
So lu'dng d o n ^ v a t sonq fsau Ituan)"
06 05 04 04 02 01 0
VIETNAM MEDICAL JOURNAL N°1 - JULY- 2017
Xac dinh dUdc LD50 cua che pham bot acid amin theo du'dng uong tren chuot nhat trang la: LDso=
14,17 ± 0,43 (g/l<g TLCT/24h). . , ,
3.2. Ket gua nghien cdu doc tinh ban tritdng dien cua bgt add amln theo dddng uong tren chuot cong trang . .. ^
3.2.1 Tinh trang Chung va sd phat trien trpng Iddng chupt cong trang: Trong thdi gian thi nghiem, chuot d ca 3 io hoat dq'ng binh thirdng, an uong tot, phan kho, i<h6ng thay bieu hien gi bat thu'dng d ca'3 io chuot trong suot thdi gian nghien cu'u.
Bang 2. Anh hu'dng ciia bot acid amin tren sU phat trien tronq iUdnq chuot conq tranq (n = 10}
Chi tieu nghien
cihi
Thdi diem Xet nghiem
Nhom nghien cu'u C x ± SD), n = 10 Chu'ng Bot acid amin Bot acid amin
(1) (140mg/l(g/24h) (2) (280mg/kg/24h) (3) Trong
lUdng
Tru'dc thi
nghiem fa)
152,83 ± 2,23 151,00 ± 9,51 163,17 ± 11,82
p>0,05 Sau 3 tuan (b)Sau 6 tuan (c) 184,83 ± 11,39
172,00 ± 8,74 164,33 ± 12,77 173,67 ± 13,98
177,83 ± 13,00
204,83 ± 22,34p>0,05 P3-,<0,0S P<0,05
Trong iudng cd the chuot (TLCT) d ca 3 nhdm nghien ciru sau 3 tuan va sau 6 tuan deu tang ri ret so vdi xuat phat diem, sU khac biet co y nghTa thdng ke vdi p<0,05. Lo dude diing thuoc lieu 2 sau 6 tuan TLCT tang nhieu hdn so vdi io thuoc iieu 1 va 16 chu'ng ciing thdi diem, su khac biet ro y nghTa thong ke vdi p<0,05.
3.2.2. Anh hu'dng cua bpt add amln dol vdi mot sd chi tieu ve huyet hpc cua chudt Bang 3. Anh hifdng ciia Bot Add Amin den so lu'dng hong cau va ham lu'dng huyet sac td cua chuot conq trang
Nhom nghjen ctfu ( x ± S D ) , n = 10 Chi tieu
nghien
cihi
Thdi diem
Xet nghiem Chu'ng Bot acid amin Bot acid amin (1) (140mq/kq/24hX2) (280mq/kq/24hX3) Hong Cau
(T/l)
Tru'dc thi nghiem(a) Sau 3 tuan (b)
6,57 ± 0,67 6,91 ± 0,57 7,71 ± 0,34 7,90 ± 0,28
p>0,05 p>0,OS
Sau 6 tuan (c) 7,72 ± 0,44 Pi.u<0,OS
P^,>0,05 TrUdc thi nghiem (a
HST (G/L) Sau 3 tuan (b)
11,90 ± 0,77 12,37 ± 0,64
14,08 ± 0,32
p>0,OS p>0,05 Sau 6 tuan (c)15,15 ± 1,32 12,65 ± 1,55 13,45± 0,59
<0,05Pi,j>0,OS Pb,c-.<0,05| p . 0 0 5 r Pb,c-,<0,05 , P^,>0,05 I ' ' > " . " ^ I P|^,>0.05 So lu'dng hong cau va ham lu'dng huyet sac to d 3 to nghien ciru sau 3 tuan deu tangsu' kiiac b]et - , i ^ —«ilr« i.t«^»« i,« K.Jj: f .^ ^n nc\ \/^\- #^..A .^-.ii C ^l.*Sn. IA ^hi'iVin l-Srtn rn r o t crt MrTv r a n Vhrii rlipm tru'dc,
hong cau giam (p>0,05). ^ , Bang 4. Anh^hudng ciia che pham bot Acid amln den so lu'dng bach cau va so lu'dng tieu cau ciia chuot
) nghTa thong ke vdi {p<0,65). Ket qua sau 6 tuan: 16 chutig tang ro ret so vdi cac thdi diem (c, su khac biet co •/ nghTa thong ke vdi (p<0,05). Lo thuoc nghien cu'u lieu 1 va lieu 2 so ludng
conq tranq Cht tieu
nghien cu'u
Thdi diem Xet nghiem
Nhom nghiSn ciTu ( x ± SD), n = 10 _. ,, , , , Bot Acid Amin I Bdt Acid Amln '-""^"9 ' ^ ' (140mo/kq;24h)(2) f280mq;kq;24h)(3) Tru'dc thi nghiem (a)
( G / l ) Sau 3 tuan (b)
6,43 ± 1,32
Sau 6 tuan (c)8,07 ± 1,81
9,34 ± 3,02 6,55 ± 1,35
10.33 ± 1,82
7.87 ± 2,58 P b „ < 0 , 0 5F>i,-, > 0,05
Pb-.c>0,05 P,..<0,05
Pb-, > 0.05
' c<0,05421.17 ± 124,55^
CG/L)
Tru'dc thi nghiem (a)~
464,50 ± 86,57 529.33 ± 73,45
Sau 3 tuan (b)Sau 6 tuan (c)
513,83 ± 60,71 474,33 ± 188,26
522,00 ± 58,83
412,83 ± 31,68
469,67 ± 90,65
424,67 ± 79,74
p>0,05 p>0,05 p>0,05 So Wdng bach cau, tieu cau d 16 chirng cd xu hu'dng tang, 2 lo thuoc lieu 1 va lieu 2 co xu_hu'ang giam so vdi xuat phat diem, tuy nhien cac gia trj deu trong gidi han binh thu'dng cua chupt cong. TUTAP CHi Y HOC VigT HAM TAP 456 - THANG 7 - SO 1 - 2017
bang 4, so sanh trong tirng 16 giii:a cac thdi diem thi nghiem va so sanh giu'a cac 16 d cung mot thdi diem, cac chi so tieu cau ciia chuot nghien ciru khong cd sir khac biet [p > 0,05).
3.2.3. Anh hu'dng ciia che pham bot Acid amln dol vdi chii'e nang gan, than cua chuot Bang 5. Anh hudnq ciia che pham bdt acid amin doi vdi hoat do enzym AST, ALT ciia diuot conq bang Chi tieu
nghien cu'u
Thdi diem Xet nghiem
Nhom nghien cu'u ( x ± SD), n = 10 Chu'ng(l) Bot acid amin
(140mq/kq/24h)(2)
Bot acid amin (28dmq/kq/24)(3) AST (U/l)
Tru'dc
thi nqhiem (a)
242,02 ± 30,79 179,72 ± 25,24 178,22 ± 40,80
Sau 3 tuan (b)Sau 6 tuan (c)
148,10 ± 29,92 133_,47± 26,21
168,98 ± 39,28 103,62 ± 31,78
251,58 ± 92,35 130.97 ± 43,36
ALT (U/L)Tru'dc
thi nqhiem (a)
67,20 ± 10,97 59,87 ± 18,17 56,95 ± 16,50
Sau 3 tuan (b)Sau 6 tuan (b)
56,75 ± 13,42
59,48 ± 8,11 101,42 ± 86,39
Pb-. < 0,05 Pb-( > 0,05 j < 0 , 0 5
Pb-i > 0,05 Pb-c < 0,05 Pc-,<0,05
Pb-i < 0,05 Ps-t < 0,05 Pt-.<0,05 Hdat do enzym _y\ST, ALT ciia 3 nhdm nghien cUu giam so vdi thdi diem trUdc nghien cirli (p<0,05) O thdi diem sau 3 tuan nghien cud, 16 chii'ng hoat dp AST giam manh, d 2 16 dung thuoc giam nhe (p>0,05), 16 thuoc lieu 2 AST tang (p<05) O thdi diem sau 6 tuan lioat dp AST d 16 thuoc li§u 1 va 2 giam so vdi cac thdi diem trUdc do (p<0,05)
I
Hinh 1. Hinh anh mo benh hoc gan chuot o cac nhom nghien cu\i (HE, lOOX) (A: Nhom chuhg (chupt sd^fj; B: Bot acid amin
140mg/kg/2'4h(chuqt so 12);
C: Bpt acid amin 2e0mg/lig/24h (chupt so 16)
Hinh 2. Hinh anh mo benh hpc than chupt d cac nhom nghien c^u (HE, lOOX) (A: Nhom chdng (chupt sd 4); B: Bot Acid Amin
140mg/l<g/24h(chupt sd 12):
C: Bpt Acid Amin 280mg/iig/24i) (chupt sd 16)) 6. Anh hddng ciia die pham bpt acid amin doi vdi nonq do Creatinin va Ure ciia chuot cong tranq Chi tieu
nghien cu'u
Thdi diem Xet nghiem
Nhom nghien cifu ( x ± SD), n = 10 ChiJTig Bdt Add amin Bot Acid amin
(14"0mq/kq/24h) (280mq/kq/24h) Creatinin
( l i m o l / l )
Tru'dc thi nqhiem (a)
Sau 3 tuan(b)
67,23 ± 4,21
78,65 ± 6,53
Sau 6 tuan(c)67,00 ± 6,66
81,00 ± 18,38
85,00 ± 3,96 60,92 ± 27,67
p>0,OS
73,37 ± 8,12 79,97 ± 7,67
p>0,OS p>0,05 Ure( m m o l / l )
Tru'dc thi nqhiem (a) Sau 3 tuan(b)
8,67 ± 1,79 7,95 ± 0,67 5,67 ±\,i
Sau 6 tuan(c)
3,80 ± 0,53
12,28 ±8,33
8,16 ± 1,93
4,32 ± 0,947,89 ± 2,35
p>0,054,01 ±1,31
p>0,05 p>0,05 p>0,05 p>0,0S p>0,OS Ket qua bang 6 cho thay, nong dp creatininva ure cd si/ thay doi nhe khi so sanh trong timg 16 giii^ cac thdi diem thi nghiem va so sanh glD^
cac 16 d ciing m6t thdi diem, tuy nhien cac chi so
deu trong gidi han binh thu'dng, sir khac biet khdng co y nghTa thong ke {p> 0,05).
3.3.4. Ket qua mo benh hpc gan, than cua chupt thi nghiem
VIETNAM MEDICAL JOURNAL N°1 - JULY- 2017 Quan sat dai the bang mat thu'dng va dddi
kfnh liip cd do phong dai 25 lan thay: mau sac, hinh thai eiia gan va than d hai 16 diing add amin khong khac so vdi chifng. _
Ket qua nghien ciid ve mo benh hpe gan, than chupt cho thay che pham bot Acid amin diing du'dng uong vdi lieu 140mg/lcg/24h va lieu 280mg/ltg/24h lien tuc trong 42 ngay, khong gay ton thu'dng tren gan, than cua chupt.
IV. BAN LUic^N
Ket qua nghien cdu doe tfnh cifp eiia bpt acid amin tiiiiy phan tir da ca Tra, ca Basa tren dpng vat thdc nghiem cho thify vdi miTc lieu 11,00 g/kg TLCr/24h {mii'c lieu cao nhat chda xuat hien chuot chet) va vdi mire lieu 17,00 g/kg TLCr/24h {mu'c lieu thap lam chet 100% so chupt), da tinh du'dc LD50 ciia che pham bot Acid amin theo du'dng uong tren chupt nhat trang la: LDso = 14,17 ± 0,43 (g/kg TLCr/24h). Hhd vay, vdi mire lieu LD50 tim dddc tuWng du'dng quy do! ra lieu tren ngu'di la l,18g/kgTLa724h.
061 vdi nghien eiru doe tinh ban tru'dng d i l n eiia bpt acid amin, vdi eac miTc lieu 140 mg va 280 mg/kg the trpng chupt cong trang/24h, quy doi ra lieu tren chupt nhat 240 mg va 480 mg kg the trpng/24h quy doi ra lieu tren ngu'di la 20 mg va 40 mg/kg the trong /24h. Trong thir nghiem nay, ehiing toi co so sanh td chdng (trddc va sau diing thuoc), dong thdi so sanh vdi nhdm doi chu'ng khong diJng thuoc. Mat khac chiing toi thiet ke hai nhom diing thuoc vdi mu'c lieu khac nhau (lieu nay gap doi lieu kia). Ket qua thf
nghiem cho thay d nhu'ng mii'c lieu cao, dirng hang ngay thdi gian dai (42 ngay) khong gay anh hu'dng den tinh trang sire khde, khong anh hu'dng den cd quan tao mau cung nhd chiit nang gan than ciia dong vat thi nghiem [6], V. KET LUAN
Oa xac dinh du'dc LDso theo du'dng udng LDj,,
= 14,17 ± 0,43 (g/kg TLCT/24h). Che pham bot acid amin cho chupt cong trang uong vdi lieu tu'dng irng _ 140mg/kg the trpng/24h va 280mg/kg the trpng/24h/kg lien tuc trong 42 ngay khong anh hu'dng den sd phat trien trong lu'dng cd the, khong doe vdi cd quan tao mau, khong anh hu'dng den chirc nang va mo benli hoc gan, than.
TAI LIEU T H A M KHAO
1. Dd Trung Dam (2014). Phu'dng phap xac djnh doc tinh cap cua thuoc. Nha xuat ban Y hoc,'tr;
154-157
2. Vien Du'dc lieu (2004). Cay thude va dpng vat lam thuoc d Viet Nam, Nha xuat ban khoa hocliy thuit- Ha Noi, tap 1, trang 245-247.
3. Turner A (1965). Sreening methods in pharmacology. Academic press, NewAcademic press, New York and London, Pages 60 - 58.
4. Abrham W.B (1978). Techniques of Animal and Clinical toxicology, Med. Pub. Chicago. Pp: 55-68, 5. WHO (1993). Research guidelines for evaluating
the safety and efficacy of herbal medicines. Manila, philipin, pages 35-41
t>. Olsson 3 and Hahn R. G (1999). Glydne toxicity after high-dose i.v, infusion of 1.5%
glycine in the mouse. British Joumal of Anaesthesias! (2): 250-254.
NGHIEN CU'U DOC TINH CAP VA DOC TINH BAN TRUffNG DIEN CUA VIEN NANG YIO TREN B6NG VAT THITC NGHIEM
Le Minh Hoang', Nguyin Hoang Ngan^, DSu Van Canh , Ph^m Xuan Phong^, Nguyin Duy BSc^
T O M TAT
Vien nang YIO dddc bao che tir Ipc nhung {Comu cervi parvum) va ddng triing ha thao {Cordycefjs milltans), da dddc danh gia tren thu'c nghiem co tae
^Daihgc YddgcCSn Tho
^Hoc vien Quan y
^Vien Yhgc Co truyen Quan doi
^Hgevien Y hgc co'truyen Viet Nam Chiu trach nhiem chinh: Nguyen Duy Bac Email: [email protected] Ngay nhan bai: 9.4.2017 Ngay phan bien khoa hoe: 29.5.2017 Ngay duyet bai: 8.6.2017
dung tot cai thien kha n3ng sinh tinh. Nham dam bao tfnh an toan va tao tien de sir dung vien nang YIO tren lam sang, che pham can Jiien hanh nghi§n ci^
ddc tinh cap va ban tru'dng dien tren dong vat thut nghiem. Phu'dng phap: xac dinh doc b'nh cap dddnq uong tren chuot nhat trSng dong Swiss theo phuWng phap Litchfield - Wilcoxon va doc tfnh ban tru'dng dien tren chuot cong trang dong Wistar. Ket qua: chua tim thay LD50 eiia vien nang YIO theo du'dng u6ng_^tren chuot nhat trang vdi miTc lieu cao nhat eo the dio ehu8t uong trong 24 gid la 25g/kg the trong. Vdi lieu 140 mg/kg/24h va lieu 420 mg/kg/24h cho chuot cong trang uong lien tuc trong 90 ngay, thuoc khong anh hddng den trong iuHng cd the va dien tim ciia chugt^
khong gay cac thay (3oi co y nghfa thong ke cac dii so
