TAP CHl Y HOC VlgT NAM TAP 459 - THANG 10 - SO 2 - 2017 biet tren theo chung toi la do benh nhan trong
nghien cu\i ciia chung tdi du'dc sang tpc eac tru'dng hdp lao khang da thuoc, mot nhdm dai dien ma eac nghien cdu tru'dc van xep vao lao phoi tai phat.
Ve can lam sang: Benh nhan co nhieu ton thu'dng xen ke, trong dd ton thu'dng xd vol chiem ty le eao (96,4%), tiep den la ton thu'dng not (89,1%), ton thu'dng tham nhiem (69,1%), ton thu'dng hang (58,2%). U Thanh Phiic [4]
thay d benh nhan tru'dc tai trj: xd hang (50,0%), not hang (29,2%). Hoang Thj Phu'dng [5] thay 95,0% cd ton thu'dng hang va xd liang d benh nhan tru'dc tai trj. Cd sy' khac nhau ve hinh thai ton thu'dng la do doi tu'dng nghien ciili d moi nghien cu'u cd si/ khac nhau. Cac nghien ciTu tru'dc cung thay sd lu'dng bach cau, bach eau lympho d benh nhan LPTP thu'dng d miTc binh thu'dng, tang bach c^u, tang ty le bach rau da nhan trung tfnh gap d mot so tru'dng hdp cd bpi nhilm vi khuan ngoai lao [2], [3]. Hb mau thu'dng thap d benh nhan LPTP cung du'dc eac nghien cu'u chi ra [6]. MuTc dp AFB(+) trong ddm d cac nghien culi tru'dc hay gap (-^) 2* va 3* [2], [3]. Mac du du'dc sang lpc lao khang da thuoc ngay tCr dau cho doi tu'dng nghien ciiXi bang xet nghiem Gene- Xpert, nhu'ng chung^toi van gap 01 tru'dng hdp MDR. Ket qua vi khuan lao khang thuoc trong nghien cuXi ciia chiing tdi thap hdn so vdi ket qua eiia cac nghien cu'u tru'dc [2], [3].
Co sir khac biet ve mu'c dp AFB va t / le khang thuoc, theo chiing toi la vi doi tu'dng MDR trong nghien cu'u cua chung toi da du'dc sang Ipc bang Gene-Xpert ngay tir (^u.
V. KET LUiSkN
Qua nghien culJ 55 benh nhan lao phoi tai phat sau lao phoi mdi tai Benh vien 74 Trung u'dng chung toi riit ra mot so ket luan sau: Dac
diem lam sang: Ty le nam: 78,2%, nO': 21,8%, tuoi trung binh: 56,98±17,01, tai phat sdm:
49,2%, tai phat muon: 50,8%. Bieu hien: Sotve chieu: 63,6%, chan an: 63,6%, met mdi; 54,6%, ho khac ddm: 74,6%. Can lam sang: Ton thu'dng xdvoi: 96,4%, not: 8 9 , 1 % , tham nhilm: 69,1%, hang: 58,2%. Bach cau d gidi han binh thu'dng:
69,1%, Hb giam: 60,0%, Hb trung binh: 119,5 ± 45,2. AFB du'dng tinh 1* chiem ty le cao hdn cae mire do khac (30,9%). Ty le khang thuoc:
51,6%, khang 1 thuoc: 62,5%, 2 thuoc: 31,3%, 3 thuoc: 6,2%, khang da thuoc (MDR): 6,2%.
Khang vdi SM; 56,5%, E: 21,7%, INH: 17,4% va khang vdi R: 4,4%.
TAI L l | U T H A M KHAO
1. B6 Y Te- CTCLQG (2015), "Hddng dan quan iy benh lad, NXB y hgc.
2. Hoang Ha (2009), NghiSn edu mot sd dac dii'm lam sang, can lam sang, smh hpc r:ua vi khuan lao d benh nhan lao phoi dieu tri lai, Luan an tien sT y hoc, Tru'cing dai hoc Y Ha Ngi.
3. Hy Ky Phooncj (2001), DSc diem iam sang, can lam sang va ket qua tai tri 6 benh nhan lao phoi tai phat tai Benh vien lao va binh phoi Ha Noi nam 1997-2000, Luan van tot nghiep BSCKII, tru'dng dai hoc YHa Noi.
4. \k Thanh Phiic {X^^^V'^Nghien edu dae diem iam sang, can lam sang va tmh hinh khang thua:
cua benh nhan dieu tn dieu tn lai sau bo tri', Luan van thac sT y hoc, Tru'dng dai hoc Y Ha noi.
5. Hoang Thj Phu'tfng (1999J, " Nhan xet nguyen nhan bo tn va mot so dae diem Jam sang, c|n lam sang qua 35 benh nhan lao phoi du'dc dieu_tri tai tri", Hoi nghi khoa hoc ve lao va benh phoi, Vien lab va benh ph6i-9/l999, tr. 45.
6. Crofts J.P., Andrews N J., Barker R.D., et al (2010),
"Risk factors for recunent tuberculosis in England and Wales, 1998-2005", Thrxax, 65, pp. 310-14.
7. Songyi H., Soonduck K., Jongsoon P., et al (2003), "Characteristics of Relapsed Patients with Pulmonary Tuberculosis Registered at Two Public Health Centers in Seoul", Korean Journal of Epidemiology, 25(2), pp. 92-99.
DANH GlA T A C D U N G DIEU TRI DAI THAO DlTffNG TYP 2 THE KHI AM LU'O'NG HU* TREN THITC NGHIEM CUA BAI THUOC
"TRI B A DIA H O A N G H O A N "
Nguyen Vinh Quoc*, Tran Cong Tru-ong*
TOM TAT
*Vien Yhoc CO truyen Quan dpi Chjii traeh nhiem chinh: Nguyen Vinh Quoc Email: [email protected] Ngay nhSn bai: 4.8.2017 Ngay phan bien khoa hoc: 29.9.2017 Ngay duyet bai: 4.10.2017
Myc tieu: danh gia tac dung cita bai thuoc Tn ba dia lipang hoan tren mo hinh eh^upt cong trang du'dc gay dai thao du'dng typ 2 the khi am ludng hi/.
Phu'dng phap nghien cihi: ^ay mo hinh chuot cong trang dai thao du'dng typ 2 the khi am lu'dng hd bang tiem phiic mac dung djch STZ 1 % lieu 30 mg/kg 1 lan duy nhat va nude sae bai thuoe ha khi lieu 9g/kg/ngay trong 14 ngay lien tyc; chuot dai: tieu chuan sau gay
VIETNAM MEDICAL JOURNAL N°2 - OCTOBER - 2017 mo hinh du'dc phan thanh 16 uong nu'dc muoi sinh ly,
16 uong glicl'azid 20mg/kg/ngay va 16 uong Tri ba dja hoang hoan lieu 16,8g/kg/ngay lien tuc trong 10 ngay; quan sat toan trang, xac djnh lu'Ong thirc an va nu'dc uong tieu thu hang ngay, can trpng lu'dng chuot, dmh lu'Ong di/dng mau, xet nghiem mo benh hpc tieu dao tuy de danh gia. Ket qua: bai thudc co tac dung cai thien cac tneu chiiYig lam sang, mire giam Glucose mau 13,7% so vdi tru'dc dieu tri tuy nhien khac biet chu^ CO y nghTa thong ke (p>0,05). Kha nang hoi phue ciia te bao tieu dao tuy kem hOn so vdi 16 doi chiJng uong Giiclazid.
Tir khoa: thi/c nghiem, dai thao du'dng typ 2, Tn ba dja hoang hoan.
SUMMARY
EVALUATING EFFECTS OF "TRI BA DIA HOANG HOAN" I N THE TREATMENT OF TYPE 2 DIABETES OF DEFIQENCY OF BOTH VITAL
ENERGY AND YIN I N THE EXPERIMENT Objective: the research was conducted for the purpose of evaluating the effects of "Tri ba dia hoang hoan" on the white rat model of type 2 diabetes mellitus in the type of yin and vital energy deficiency in the experiment. Method: rats were caused diabetes by STZ 1% at 30mg/kg body weight/day and decoction of "Ha khi" formulas during 14 days; rats successfully caused diabetes were divided into 3 groups: oral saline group, glidazid 20mg/kg/day group and "Tn ba dia hoang hoan" 16.8g/kg/day group in 10 days; health condition observing, food and drink daily consumed amount determining, mouse weighting, blood glucosequanbfying, histopathological pancreatic islets examining were methods used to evaluate the effects of medication. Results: The traditional medicine formulas improved clinical symptoms, reduced 13.7% blood glucose, however the difference was not statistically different (p>0.05). The recovery ability of pancreahc islets of study group was less than Glidazid group.
Keywords: experimental animal; type 2 diabetes, Tri ba dia hoang hoan.
L OAT VAN DE
Trong nhutig nam gan day, cung vcli dieu kien song du'dc nang eao, benh dai thao du'dng (OTO) cung du'dc phat hien vdi ty le tang dang ngai: khoang 300% trong vong 10 nam trd lai day. Tai Viet Nam, ty le mac benh DTO khoang 2,7%. Tdi nam 2025, diT bao toan eau se co khoang 380 trieu ngu'di mac benh, trong dd 95%
la €)T£) typ 2. Benh DTO tien trien lau ngay neu khong du'dc kiem soat tot se dan den eac bien chu'ng tai cae cd quan dich nhu" nao, tim, than, mat, mach mau va than kinh ngoai bien... [1]. Y hpc CO truyen vdi bien chu'ng luan tri sau sac va phu'dng du'dc phong phu da va dang the hien nhu'ng net u'u viet trong dieu tri chu'ng tieu khat - j D p t chu'ng benh cd nhieu diem tu'dng dong vdi benh OTO. Oa ed nhieu bai thuoe vi thudc du'dc
su" dyng du'dc nghien cuU de dieu tri benh nay va da eho mot so ket qua kha quan.
Oe tai "Hrdp tie nghien cuti mot so cd phddng de dieu trj mot sd binh do rdi loan chuyen hoa tren thu'c nghiem va lam sang" la nhiem vu hdp tae quoc te ve khoa hoe va eong nghe theo nghi dinh thu' du'dc Bp Khoa hoc va Cong nghe. Bo Quoc phdng giao nhiem vu eho Vien Y hoc co truyen Quan dpi thu'c hien. "Tri ba dja hoang hoan" la mot trong 05 bai thuoe eo phu'dng diftJc Hgi dong xet duyet thuyet minh lu'a chpn de nghien culi doi vdi benh ly OTO typ 2. Chung toi tien hanh nghien cu'u nay vdi muc tieu danh gia tac dung ha du'dng huyet ciia bai thuoe nay tren mo hinh dpng vat thi/e nghiem gay OTO typ 2 the khi am luSng hu'.
II. t)6l TU'ONG VA PHUONG PHAP NGHIEN OjU 2 . 1 . Dong vat thu'c nghiem: Chuot cong trang du'c Wistar, trpng lu'dng trung binh 200 ± 20g (tu'dng du'dng 8 tuan tuoi), du'dc cung cap bcfi Ban chan nuoi - Hoc vien Quan y. Chuot du'dc nuoi trong dieu kien phong thi nghiem tai khoa Nghien cull thy'c nghiem - Vien YHCT Quan doi.
2.2. Bai thuoc nghien cu'u
- Bai thuoc CO phu'dng Tri ba dia hoang hoan:
Thue dia: 15g; Hoai sdn: 15g; Mau ddn bl: ISg;
Hoang ba: 15g; Trach ta: 15g; Sdn thu: 15g;
Phuc linh: 15g; Tri mau: 15g.
- Bai thuoc gay ha khi: Thanh bi 18g; Chi xac 18g; Phu tij- che 15g [ 2 ] .
Hai bai thudc tren du'dc sae va co den ty le 3/1 (3gam du'dc lieu lay 1ml eao).
2.3. Phu'dng phap tien hanh 2.3.1. Thu'c nghiem giai doan 1: gay mo hinh ehupt DTD typ 2 the khi am lu'dng hu': Can trpng lu'dng ehupt tru'dc khi thu'c nghiem, cho ehupt an thCre an giau nang lu'dng thdi gian 21 ngay, khong han che lu'dng thu'c an.
Ngay thu" 22, tiem phiic mac dung dieh ST2 1 % lieu 30 mg/kg the trpng - 1 lan duy nhat.
Sau khi tiem STZ, eho ehupt an thu'c an pho thong trong 14 ngay. Sau do cho chuot uong nu'dc sac bai thuoe ha khi lieu 9g/kg the bpng 1 lan/ ngay trong 14 ngay lien tuc de gay mo hinh chu'ng tieu khat the khi am luSng hu' theo Y hoc CO truyen [ 2 ] .
2.3.2. Thu'c nghiem giai do^n 2: chuot dat tieu chuan nghien cuxi du'dc chia thanh 3 15, moi
16 16 chuot: ^ - Lo 1: uong nu'dc muoi sinh ly lOml/kg the
trong x 1 Ian/ngay.
- Lo 2: udng glidazid 20mg/kg the trpng x 1 Ian/ngay,
212
TAP CHi Y HOC VlgT NAM TAP 459 - THANG 10 - SO 2 - 2017 - Ld 3: udng Tri ba dja hoang hoan lieu
16,8g/kg X 1 Ian/ngay.
Chuot du'dc uong thuoc vao luc 8h lien tuc trong 10 ngay.
Trong qua trinh nghien eulJ thu'c nghiem giai doan 2, cac chuot dUdc nudi dudng bang thu'c an pho thong.
2.4. Chi tieu quan sat va danh gia - Quan sat toan trang: Hang ngay quan sat cae hoat dpng cua ehupt, ghi nhan cac thay doi ve van dpng, tinh than, mau sac, tinh trcing long, hinh the, each an uong, tfnh chat phan, nu'dc tieu.
- Xae dinh lu'dng thu'c an va nu'dc udng tieu thu hang ngay, trpng lu'dng chuot: vao cac thdi
diem tru'dc va sau uong thudc nghien cu'u.
- Djnh lu'dng du'dng mau bang may thu" du'dng huyet nhanh: vao cac thdi diem tru'dc khi udng thuoc va sau uong thuoe nghien euli 10 ngay.
- Xet nghiem md benh hpc tieu dao tuy: sau khi ket thuc thy'c nghiem, lay mau lach cua cac 16 ehupt lam tieu ban md benh hoc.
2.5. Phu'dng phap thong ke: Theo thuat toan thong ke bang phan mem SPSS 16.0 for Windows. SLT dyng cae thuat toan: tinh so trung binh; tinh dp lech ehuan (SD); so sanh 2 gia tn trung binh dung T- test Student. Khac biet co y nghTa khi p<0,05.
in. KET QUA NGHIEN CLTU
3.1. Cac chi tieu quan sat chung tren chuot thu'c nghiem Bing 1. Tinh trang ehung eua chuot tru'dc va sau uong thuoc Lo chuot
Nu'dc muoi sinh ly Glidazid Tri ba dia hoanq hoan
Trang thai tinh than Cham chap Binh thu'dng Binh thu'dng
Trang thai van aonq Nam tai cho, it
van donq Hoat dpng binh
thu'dng Hoat dpng binh
thiidnq
Mau sac, tinh tranq lonq Long khdng bdng, thd
rap, xu, runq lonq Bdng, mu'di, mem Bdng, mu'di, mem
Dai tien Tao Binh thu'dng Binh thu'dng
Tieu tien Tieujtien
nhieii Tieu tien binh thu'dng
Tieu tien nhieu Nhin xet: tru'dc khi tien hanh thy'c nghiem ^iai doan 2, ehupt d cac 16 deu xuat hien trang thai tinh than cham chap d cac mu'c dp kliac nhau, nam co quap, it van dpng, Idng xu khdng bdng, rung long, dat tien tao, tieu nhieu i/dt miin eu'a Idt day long. O giai doan 2 ciia thy'c nghiem, sau 10 ngay uong tliuoc, chuot d Id udng giiclazid va bai thuoe co phu'dng Tri ba dia hoang hoan nhan thay eac bieu hien lam sang eua ehupt cd xu hu'dng cai thien tot. Cae ehupt trd lai hoat dpng binh thu'dng, long bong, mu'dt, mem, dai tien het tao. Tuy nhien ehupt d 16 udng Tri ba dia hoang hoan nhan thay mu'c dp tieu tien khdng thay doi so vdi tru'dc dieu tn va vdi Id udng nu'dc m u ^ s i n h ly.
Bang 2. Ltffflg thu'c an t i j u t h u cua chuot [ g / l O O g trong lu'dng; A- ± S D ) Lo chuot
Nu'dc muoi sinh ly
So lu'dng Tru'dc uong thuoc Sau uong thuoc I p (tr-s)
16 6,12 ± 1,24 6,25 ± 1,36
>0,05Glidazid
16 5,21 ± 1,12
>Q,05Tri ba dja hoang hoan
5,41 ± 1,32
>0,05
Nhin xet: khong thay sy' thay doi ve tieu thy thu'c an tru'dc va sau nghien cu'u giii'a cae Id ehupt uong nu'dc muoi sinh ly, Glidazid va Tri ba dja hoang hoan(p>0,05). „
Bang 3. Tieu t h y nu'dc uong ciia chuot ( m l / l O O g trong lu'gng; x ± S D )
Lo chuot So lu'dng Tru'dc uong thuoc Sau uong thuoc P ( t r - s ) Nu'dc muoi sinh ly
Giiclazid
16 17,5 ± 2,1 18,1 ± 2,e
12,9 ± 5,6
>0,05
<0,05
16 18,6 ± 4,5
I7,l ± 3,9 I 17,2 ± 3 , 5 ' j > q ^ _ ,
*.• p<0,05 khi so vdi io dung Gliciazide tsi cung thdi diem.
yVAa/fxef.'khdng cd su* thay ddi ve tieu thu nu'dc tru'dc uong thuoc giu'a cac Id ehupt uong nu'dc muoi sinh ly,' Glydazid va Tri ba'dia hoang hoan. Lu^dng nu'dc tieu thy d Id udng giiclazid sau uong thudc thap hdn cd y nghia so vdi 16 uong nu'dc muoi sinh ly va Tri ba dia hoang hoan (p<0,05). Lu'dng nu'dc tieu thy 616 udng Tri ba dia hoang hoan va 16 uong nu'dc mu^sinh ly khong khac biet vdi p>0,05.
Bang 4 . Tronq lu'dnq chugt d cac Id ( g a m ; x ± S D ) Tri ba dja hoang hoarT
Sau uong thuoc Lo chugt So lu'dng I Tru'dc uong thuoc
267,6 ± 20,1
Nu'dc muoi sinh ly
16 245,8 ± 15,1
216,0 ± 18,3
Glidazid
239,2 ± \SJ_
228,5 ± 18,2
>0,Q5
Z 1 0 , U X l O , .
212,5 ± 17,i
>0,05 Tri ba dia hoanq hoarT
>0,05
>Q,05VIETNAM MEDICAL JOURNAL N°2 - OCTOBER - 2017
/VAan jref/khong CO sy" thay doi ve trpng lifdng cua ehupt tru'dc va sau 10 ngay uong thuoc gjfe cac 16 uong nu'o'c niuoi sinfi ly, gliciazld va Tri ba dia hoang haan(p>0,05).
3.2. Ket qua x e t n g h i e m du'dng mau
Bang 5. Ket qua x e t n g h i e m Glucose m a u ( v ± S D )
Sd I Glucose mau ( m m o l / l ) | Ty le
J u ' a n g _ Tr"IFdcTrc "~[ Sau NC ' g i a m (°/o) P ^^^'^) Ld chuot
Nude muoi sinh IY__
16
GiiclazidTn ba dia hoang hoan
16
15,0 ± 5,2 14,6 ± 4,7
'': p<0, Nhan xet: bai thuoc Tn ba dia hoang hoan CO tac dung lam giam nhe dydng huyet d miTc 13,7% so vdi tru'dc dieu tri, mu'c bien doi chi/a CO y nghia thong ke so vd: thdi diem tri/dc khi udng thudc vdi p>0,05. Mdc bien doi Glucose mau sau khi dieu tn cua Tn ba dia hoang hoan khong khac biet vdi 16 chuot diing nyde muoi sinh iy (p>0,05).3.3. Ket qua x e t n g h i e m md benh hpc;
Ket qua giai phau mo benh hoc tuyen tuy phii hdp vdi eac tneu chu'ng lam sang va cac xet nghiem can lam sang. Sau 10 ngay dieu trj, d Id chuot uong bai thuoe Tri ba dia hoang hoan va Id ehupt uong nu'dc muoi sinli ly nhan thay cd khoang 40% te bao dao tuy thoai hda, chu vi tieu dao hep, dirng sat nhau. Kich thu'dc te bao Idiong deu, mot sd te bao cd bao tu'dng thoai hoa, mat cau triic dang hat, bat mau eosin manh, nhan te bao khdng deu, mdt so nhan to.
Trong khi do, 16 ehupt thu'c nghiem uong Glidazid cd bieu hien hoi phue te bao tieu dao tuy the hien d so lu'dng te bao thoai hda cdn khoang 20%, mot so te bao ed nhan khong deu.
14,6 ± 4,8 l o r ± 5 5
2,7 29 E
1
>p,05
<0 05
Anh 1. Te bio tieu dao tuy id udng nu'dc mudii "
Anh 2, TS bao tieu dao tuy Id udng giiclazid (HEx 400)
05 kiT_jo I'd lo dung Glcia^ de ta cung th'Fd&ii
Anh 3. Te bao tieu dao tuy lo uong Tn ba dia hoang hoan (HE x 400) IV. BAN LUAN
4 . 1 . Md hinh nghien cu'u va k@t qua:
Phu'dng phap gay mo hinh OTO tren dpng vat thy'c nghjem bao gom phu'dng phap su'dung hoa chat, phau thuat hoac bien ddi gen...[2]'. Tror^
nghien euli nay ehung tdi sir dyng hda ehat STZ ket hdp vdi bai thudc ha khi theo ly luan Y hoc CO truyen de gay OTO the khi am ludng hu'trSn thye nghiem. Cac ket qua thu du'dc d giai doan 1 cua thu'c nghiem ve bleu hien lam sang, ket qua xet nghiem glucose mau d thdi diem sau liem STZ ket hdp uong thudc ha khi, ket qua xet nghiem mo benh hpc tuyen tuy cua ehupt thu'c nghiem dddc xac djnh la dat yeu cau so vdi muc tieu cua thy'c nghiem [ 3 ] .
4.2. Bai thuoc nghien ed'u: Y hpc co truyen eho ranq^ ban ehat OTO la do the chat am hu", am hu" de hda tao nhiet lam tdn thu'dng phan am cang nang. Am phan ton thu'dng d l gay hao Idii, khf suy lam cho huyet djch van hanh khdng du^c thdng dat tir do dan tdi' chu'ng khi am ludng hiT, huyet mach d trd [ 4 ] . Cac nghien cihj t i i ^ nghiem da chuTig minh cac bien chufhg t i ^ thu'dng than kinh do OTO la do tang dp nhjft mau, tang ket tap tieu cau, giam lull ludng tuan hoan, dac biet la tuan hoan ngoai vi..., vikdffiu tn can het su'c chu trpng ich khi dudng am, tiTbo can than, hoat huyet hda tr thdng lac [5]^ BaJ thuoc Tri ba dja hoang hoan gom cac vi thuoc l»
than du'dng am sinh tan djch, thanh nhiet ..,mgt sd nghien ciili da chirtig minh bai thuoe co tac dung ho trd dieu tri DTO [6], [7].
Trong nghien eiiu eua cliung tdi, sau 10 ngay uong thuoe Tri ba dja hoang hoan lieu 16,8g/lt9
TAP CHl Y HOC VlgT HAM TAP 459 - THANG 10 - SO 2 - 2017
•• the trpng thay ehupt ed eai thien trang thai tinh than, van dpng, tinh trang Idng, dai tien het tao.
Tuy nhien tinh trang tieu nhieu van chu^ du'dc ' cai thien. Tac dyng ha glucose mau tren ehupt d ,1 muTe 13,7% so vdi tru'dc dieu trj, mu'c giam nay
> chu^ cd y nghia thdng ke so vdi tru'dc dieu tri, co
^ the do thdi gian nghien ciru ngan nen tliuoe
^ chua the hien tac dung mot each ro ret, theo
^ chiing toi can cd them cac nghien culi khac vdi
^ thdi gian durig thuoc dai hdn de cd danh gia j khaeh quan. O Id chiTng du'dng sir dung glidazid I thay lam giam glucose mau ed y nghTa so vdi f tru'dc dieu tn (p<C,05). Day la thudc tan du'dc
% thu'dng du'dc SLT dung tren lam sang dieu trj DTD
I
typ 2 nen cung the hien rd tac dung tren thy'c nghiem. Ket qua xet nghiem m6 benh hpc tuyen tuy d Id udng Tri ba dja hoang hoan thay rang Wia nang hoi phuc cua te bao tieu dao tuy kem ' hdn so vdi Id dol chuTig udng Giiclazid, ket qua nay cung phii hdp vdi hieu qua ha glucose mau cita ehupt thUc nghiem du'dc trinh bay tai bang 5 . trong nghien culi cua chung tdi.3 V. K ^ LUijiN
Bai thudc Tri ba dia hoang hoan trong thdi gian uong thuoc 10 ngay vdi lieu 16,8g/kg the tnDng chda thay cd tac dung ha dudng h u y ^ rd ret tren thy'c nghiem md hinh DTO typ 2 the khi am . ludng hu'. Kha nang hoi phue cua te bao tieu dao , tuy kem hdn so vdi 16 ddi chimg udng Giiclazid.
TAI LI§U T H A M KHAO
1. Do Trung Quan (2006). Bien ehuhg Dai thao dddng va dieu tn, NXB Y hoe. Ha Noi.
2. R g (2005). 'P0m^ '^miff '^SM, AgOl
^^m^, =itm.
Banh Thanh (2005). Phu'dng phap nghien ciTu thu'c nghiem Y hpc co truyen. NXB Y hoc, Bac kinh.
i. Nguyin Vinh Quoc (2013). Gay mo hinh dai thao dirdng typ 2 the khi am ludng hU tren ehupt cong trang. Tap chi Yhoc Vletnam,Z, 50-55.
4. 4=EEJS^, 4 ^ ^ f l : (2007). '^mtm ifflS32^?7
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NGHIEN Cii'u T A C DUNG AN THAN, GlAl LO AU C O A CAO L 6 N G DU'O'NG TAM AN THAN TREN THU'C NGHIEM
Nguyen Van Tam*, Nguyen Tran Giang Hu-ffng**, Ph^m Thi Van Anh**, D6 Thi Phuong**, Nguyin Thi Thanh Loan**
epng nang cao; lam giam thdi gian bam tren true quay Rotarod va tren mo hinh do sire bam. Ngoai Va, cao Idng Dudng tam an than lam giam so lln ehupt di chuyen theo chieu ngang va theo chieu dpc tren mo hinh do hoat dpng ky. Ket luan: Cao long Du'dng tam an than lieu 13,3 ml/kg/ngay va lieu 39,9 ml/kg/ngay the hien tac dung an than va giai lo au.
Tir khoa: Cao Idng Dudng tam an than, an than, giai lo au, dpng vat thUc nghiem.
SUMMARY
EVALUATE SEDATIVE AND ANXIOLYTIC EFFECTS OF THE "DUONG TAM AN THAN"
EXTRACT I N ANIMALS Objectives: The "Duong tam an than" extract was used to evaluate sedative and anxiolytic effects in animals. Methods: The sedative and anxiolytic effects were assessed using an elevated plus maze, rotarod test, home - cage activity and grip strength test.
TOM TAT
Muc tieu: Nghien culj du'dc thu'c hien nham danh gia tac dung an than, glai lo au e^a cao long DuSng tam an than tren thu'c nghiem. Phu'dng phap nghien cu'u: Tac dung an than, giai lo au du'dc danh gia tren mo hinh dau epng nang eao, mo hinh true quay Rotarod, mo hinh do hoat dpng ky va md hinh do su'c bam. Ket qua: Cao long Dudng tam an than Iam tang cd <i nghTa thdi gian IUIJ lai nhanh md, tang s5 lan vao nhanh md cua cJiupt tren mo hinh dau
*Benh vien Y hgc td truyen Thanh Hoa
* * Trddng Dai hpc Y Ha Noi
Chju trich nhiem ehinh: Nguyen Thj Thanh Loan Email: [email protected] Ngay nhan bai: 19.7.2017 Ngay phan bien khoa hoc: 15.9.2017 Ngay duyit bai: 3.10.2017
