T a p chf K h o a hoc va C o n g n g h e B i e n ; T a p 14, S o 2 ; 2014: 155-162 I S S N : 1859-3097 htQj://www.vjs.ac.vn/index.php/jmst
ANH HUdNG CUA MANNAN OLIGOSACCHARIDE BO SUNG VAO
THirC AN LEN PROTEIN TRONG CO, HINH THAI RUQT VA TE BAO MAU CUA CA KHOANG CO NEMO, AMPHIPRION OCELLARIS
D o H u n H o a n g ' , H o a n g Dire L v , P h a m X u a n Ky, D a n g T r a n Tii T r a m , N g u y e n Thi K i m Bich, H o S o n LSm, T r a n V a n H u y n h , D a o Viet H a , N g u y i n T h u H 6 n g , P h a n B a o V i
V i e n H a i d u o n g h p c , V i e n H a n lam Khoa hgc va C o n g n g h e Viet N a m E-mail: d o h u u h o a n g 2 0 0 2 @ y a h o o . c o m
N g a y nhan bai: 17-2-2014
TOM TAT: Cd khoang cd (Amphiprion ocellaris) (-2,4 cm) duac nudi trong he thing loc tudn hodn thi tich 50 lit, mat do 20 con/be vd cho dn trong 10 ludn bdng cdc thirc dn co bo sung 6 nong dg mannan oligosaccharide (MOS): ddi chicng. 0.05%; 0.10%; 0.15%; 0,20%; 0.25%vd Id bdsung 0,30%. Cdc chi tieu do dge bao gom. ty le chu vi ben trong vd ben ngodi thdnh rugt. hdm lugng protein trong ca vd ti bdo mdu long so Kit qud nghien cicu cho thdy, ty le chu vi thdnh ruol cd cao nhdt d 16 cd hdm luang MOS O.I0%vdO.l5% ly le ndy thdp nhdt d 16 doi chimg vd 16 bd sung 0,3%
MOS (P < 0,05). dndng do thdp hon hodc cao han 0.1 - 0,15% cd diu co dien tich be mat thdnh rugt thdp (P < 0,05). Sau 4 tudn nuoi hdm lugng protein trong ca cd dao dong tic I2,85%P den 15.53%P. Protein trong ca cd d tudn thic 10 cao han so vdi ludn thu 4, nhung khong sai khdc (P>0.05) vd hdm lugng protein ciing khong co khdc nhau co y nghTa thdng_ ke giira cdc 16 thi nghiim dcdc ldn do (tudn I vd tudn 10) (P < 0.05) Tdng s6 te bdo mdu cd d tudn thic 10 cao nhdt d 16 cho an 0.05% (26.22^l(f ± 2,80^I(f tb/miyC) ki den Id nghiem thicc bd sung 0,25% MOS m 0,10%
MOS dat gid iri ldn lii^ la 2l,54^l()^±3,65xld'tb/mm'yd 20,73^10'± 7,88x10^ tb/mrr/ Mat do te bdo mdu thdp nhdt d Id cd cho dn 0.15% MOS (11.02^10' ± 2.00^l(f ib/mn^). Mat do te bdo mdu sai khdc coy nghta thong ke giiai Id cho dn 0.05% MOS (Id 2) so voi 16 ddi chimg MOS vd Id cd bo sung 0,15% MOS (P<0.05).
Tie khoa: Cd khoang c6, Amphiprion ocellaris. mannan oligosaccharide, hinh thai rugt, protein, ti bdo mdu.
M d D A U k h a n g sinh hoac hda c h i t t h u o n g d u o c su dung.
D a y la giai phap tuc thai va tac dgng xau toi Ca khoang c o thuge h p P o m a c e n t n d a e la ^ ^ ^ xruang. Nhieu c o n g trinh nghien c u u da loai ca canh bien c o gia tri t h u o n g mai va ^hung minh b o sung prebiotics co th^ lam tang chiing d u g c nuoi lam canh p h o bien vi chiing ^^^ ^ g ^-^^ trucmg va sue khoe vat nuoi. Tuy CO mau s k dep Loai ca k h o a n g c o n e m o ^ j ^ ^ ^ ^ ' ^^^^ ^^ ^^^^ j^j^h j^g^jgn ^uu b 6 sung {Amphiprion ocellaris) d u g c cho sinh san nhan ^^^ '^^^^^ ^-^^^ ^^^^ ^^^ ^,j^j ]^ mannan t^o thanh c o n g trong dieu kien nuoi nhot. T u y oligosaccharide len ca khoang c6.
nhien trong qua trinh nuoi ea t h u a n g b o an, ca _ con cham lan, ty le s 6 n g thSp. Vi vay, tim kiem Prebiotics la loai d u o n g chat chuyen bi?t
giai ph'ap n a n g c a o tdc d o sinh t r u o n g va ty le danh cho mot so n h o m sinh vat co lgi cho c o s6ng ca khoang c 6 c o n la v§n d^ dn th.£t hien the vat chu. T h o n g qua viec c u n g cap dinh nay T r o n g qua trinh nuoi, khi ca bi benh, d u o n g mot each c o c h g n lpe cho mgt hoac mgt
D6 Huu Hoang, Hoang Bice Lu,.
so vi sinh vat co lgi trong duang mot, prebiotic lam thay d6i co chgn lpe he vi sinh vat duong rupt ciia vat chti. Prebiotic con dugc xem la chat kich thich he mien djch cua vat chu [16].
Mannan oligosaccharide (MOS), la mpt loai prebiotic tu nhien, dugc chiec xuat tii thanh te bao nam men (Saccharomyces cereviciae), dugc su dung nhieu trong ehan nuoi gia siic, gia cam. Ket qua nghien cuu cho thay bo sung mannan oligosaccharides vao thuc an da cho nhieu hieu qua tich cue doi vai nhieu loai thuy san khac nhau nhu: ca bon [7], ca tam [3], ca trap [10]; ca da tron [15], ca hoi [6, 7], ca chep canh [1,2].
Nham cung cap eo so khoa hpe cho viec su dung Mannan oligosaccharide (MOS) bo sung vao thuc an de tang cuong siic khoe ciia ca nuoi, chiing toi bo tri thi nghiem nghien cuu anh huong ciia cac nong dp MOS bo sung vao thiic an len hinh thai rugt, ham lugng protein va te bao mau ciia ca Ichoang co nemo, Amphiprion ocellaris o giai doan con non.
VAT LIEU VA PHU'ONG PHAP NGHIEN CUtJ
H§ thong nuoi va ca thi nghiem
He thong nuoi: tong so 21 be thuy tinh, the tich 50 lit, phan chia thanh 3 he thong loc tuan hoan, tu do chiing toi do eae yeu to moi truong theo 3 he thong nay. Moi be nho va be Ige tuan hoan deu co gan he thong sue khi 24/24. Toe do nucrc chay o moi be khoang 0,5 lit/phiit. Su dung ca khoang co nemo (Amphiprion ocellaris), khoe manh co kieh thuoc trung binh 24,15 mm, chgn Ige tir trai san xuat giong ca khoang co, phong Cong nghe nuoi trong. Thf nghiem tien hanh tai Tram thirc nghiem va co so nuoi ciia phong Cong nghe Nuoi trong Vien Hai duong hoc tu thang 5/2013 den thang 11/2013.
Chuan bj thirc an thi nghiem
Sir dung thiic an cong nghiep Lansy (dam 40%, lipid 9%, 2,5% xa, kich thuoc hat - 0,8 mm) lam thiie an doi chiing. Thiic an tren CO ham lugng va thanh phan dinh duong ehinh phu hop cho ca canh [14, 17]. Sii dung eh^
pham thuong mai Bio-Mos^ (Allteeh, USA).
Sir dung 6 ham luong MOS khac nhau (0,05;
0,10; 0,15; 0,2; 0,25; 0,3%) bd sung vao thiic
an t6ng hgp Lansy, d^ kho va bao quan o 4"C cho den khi su dung.
Bo tri thi nghiem
Ca thi nghiem dugc tha ngau nhien vao b^
thi nghiem voi so lugng 20 con moi be va dugc nuoi thich nghi 7 ngay truac khi tien hanh thi nghiem. Thf nghiem bao gom 7 nghiem thiic vii 3 lan lap cho moi nghiem thuc. Tung nhom 3 bi thf nghiem dugc chgn ngau nhien va cho an 1 loai thiic an co ham lugng MOS tren va nuoi trong 10 tuan. Thu mlu dem te bao mau (2 tuan/lan), protein trong co phan tich liic bat dau, tuan 4 va tuan 10, thu mau rugt g tuan thu 10.
Cham soc va quan ly ca thi nghiem Ca se cho an moi ngay 2 lan vao buoi sang va buoi chieu. Lugng Ihiie an dugc cung cap theo nhu cau cua ea bang each quan sat true tiep. Trong suot thai gian thi nghiem: nhiet do dao dgng trong khoang 28,5 - 29,9"C, pH dao dpng trong khoang: 7,8 - 8,1. Dp man nam trong khoang 33,81 - 34,45%o va NH4/NH3 nSm trong khoang 0 - 0,25 ppm.
Thu thap va xii' ly so lieu
Ty le chu vi trong vd ngodi thdnh rugt: Mau rupt ca thu m6i be 1 con (3 con moi nghiSin thiic) vao luc ket thiie thi nghiem (tuan 10).
Giai phau va co dinh rupt ca trong dung dich formol 10% truac khi tien hanh cdt tieu ban.
Tieu ban dupc quan sat duoi kinh hi^n vi Olympus CX 31 6 dp phong dai 100 lan va duoc chup bang may anh Olympus E330. Do chu vi mat trong va ngoai thanh rugt (moi 16 do 5 mau) cua ca bang phan mem ImageJ, theo phuang phap ciia Dimitroglou et al. [6]. Ty le chu vi ben ngoai va chu vi ben trong thanh nigt (PR) dugc tinh theo cong thiic: PR = a/b.
Trong d6: a: chu vi trong ciia rugt.; b: chu vi ngoai cua rugt.
Hdm luang protein trong cct cd khoang co nemo: Mau phan tich protein trong eo ca dupc thu vao liic bit dau thi nghiem, tuSn thir 4 va tuan thii 10. Moi bk thu ngiu nhien 1 ca the, tong so mau cho mdi nghiem thirc la 3 6 moi lan thu mau. Ham lugng protein tdng s6 trong ea ca dugc phan tich dua theo phuang phap ciia Bradford (1976).
Te bdo mau long so: Mat do te bao mau ca dugc thu luc ket thu thi nghiem, moi bi thu ngiu nhien 1 ca the, s6 mlu la 21 ca t h i Mau dugc thu tir tmh mach duoi bing ong tiem 1 mL, CO chiia san 0,2 mL nuac muoi sinh ly lam chat chong dong mau. The tich mau thuc te moi ISn hut dugc ghi lai de tinh ty le pha loang.
Dem mau b5ng buong dem hong eau Neubauer Improved Bright-line duai kinh hien vi o dp phong dai 400 lan. Mat do hong cau dugc tfnh theo cong thiic sau: Mat do te bao mau (HC/mm^) = A X 4000 x B/SO. Trong do: A:
tong so lugng te bao mau dem dugc tren 5 6 dem Ion hay 80 6 dem nho (moi 6 dem lan co 16 6 nho); B: he so pha loang; The tfch 1 6 dem nho la 1/4000 mm^ (dien tich 1 6 nho:
1/400 mm^, chieu day tir mat buong dem den lamelle: 1/10 mm).
Thong ke so lieu. Tfnh toan gia trj trung binh, sai so, ty le song bang phan mem Excel.
So sanh ham lugng protein trong co, ty le chu
Anh hucmg cua oligosaccharide bd sung . . VI thanh rupt va so lugng te bao mau cua ca nuoi giira cac 16 thi nghiem bang phuang phap phan tich phuang sai (ANOVA). Gia tri P <
0,05 duac xem la khac nhau eo y nghTa thong ke giira cac gia trj trung binh.
KET QUA
Mot so yeu to moi tnrcmg trong be thi nghiem
Qua bang I thay rang trong suot thoi gian thi nghiem nhiet dp dao dgng trong khoang 28,5 - 30''C, pH dao dpng trong khoang: 8 - 8,3. Dp man nam trong khoang 34 - 35%o, NH4/NH3 nam trong khoang 0 - 0,25 mg/L va NO3 dao dpng tu 0 - 0,25 ,mg/L. Theo cac nghien cim truac day, cac yeu to moi trudng trong be thi nghiem (bang 1) deu nim trong ngu'ong cho phep va khong anh huong den sir tang truong va ty le song cua ca khoang co nemo (Ha Thi Le Loc, 2005).
Nhl^t d 9 (°C) 28,5 - 30°C
B a n g L C a c y e u t o m o i t r u o n g 1 D o m^n [%o) pH
3 4 - 3 5 8 - 8,3
:rong be thi nghiem.
NH4/NH3 (mg/L) 0 - 0 , 1
NO] (mg/L) 0 - 0 , 2 5
Hinh thai ruot ciia ca nemo khi cho an cac ham lu-^Tig MOS khac nhau
Bang 2: Ty le chu vi ben trong va chu vi ben ngoai thanh mgt (PR) ciia ca nemo 6 tuan 10
Lo thi n g h i e m 1 2 3 4 5 6 7
PR 1,66 ± 0,71"
1,72 ± 0 . 0 9 "
2 , 1 7 * 0 , 0 9 "
2,12 ± 0 , 1 5 "
1,8010,05"
1 , 7 0 1 0 , 1 1 "
1,6210,05"
Ld /• doi chimg. Ld 2: 0.05%. Lo 3: 0.10%. Lo 4. 0.15%, Lo 5: 0.20% Lo 6. 0.25%. Lo 7: 0.3%
So heu trinh bdy la gid trj trung binh ± sai sd.
So lieu cimg cot co cdc chic cdi khdc nhau the hien sai khdc coy nghTa thong ke (P <0,05)
Ty Ic giira chu vi ben trong va chu vi ben ngoai dat gia tri eao nhat a 16 3 va 4 {lan lugt ia 2,12 ± 6,15 va 2,17 ± 0,09) va thap nhlt a 16 I va io 7 (gia tri \kn lugt la 1,66 ± 0,71 va 1,62 ± 0,05). Ty le nay khong co su sai khac co y nghia thong ke giira cac 16 1, 2, 5, 6 va 7 (P >
0,05) va giua 16 3 va 16 4 (P > 0,05). Tuy nhien, ty ie giira chu vi ben trong va chu vi ben ngoai ciia ca 6 16 3 va 16 4 (0,10% va 0,15%
MOS) cao hon so voi cac 16 con lai (P < 0,05) (bang 2, hinh 1).
Do Hihi Hoang, Hoang Diic Lu,.
Hinh 1. Lat cat ngang mo rugt ca khoang c6 nemo 6 tu^n 10
Trong dd A. B. CD. E. Fvd H Idnhcnt Id cdc 16 ihi ngluOm 1.2. 3. 4. 5. 6 & 7 Ld I ddi chimg. L6 2:
0.05% Lo 3: 0.10%. La 4. 0.15% Lo 5 0,20"/,, Lo 6: 0.25%. Lo 7:0 3%
Him lu'9^g protein trong cof ciia ca nemo Ham lugng protein trung binh ciia ca luc bat dau thi nghiem la 12,33%. Sau 4 tuSn cho an, h l ^ lugng protein thap nhat o 16 ddi chiing (161) va 16 3 (0,10% MOS) gia tri lan lugt la 12,85%P va 12,64%P; ham lugng protein cao nhit 6 cac 16 4 (0,15% MOS) va 16 5 (0,20%
MOS), lan lugt la I5,34%P va 15,53%P. Ham lugng protein co xu huong tang theo thai gian (tuan 10 cao hon tuan thit 4) trong ciing 1 16 thuc an. Tuy nhien, kk qua phan tfch protein 6 tuan thii 4 va tuan 10, khong co su sai khac eo y nghTa th6ng ke giiia ham lugng protein trong CO ca khoang co nemo a cac 16 thi nghiem khac nhau (P > 0,05). Duy nhat nhom ca nemo an b6 sung 0,2% MOS c6 ham lugng protein trong ca 6 tuan 10 cao han co y nghTa so vai tuan thii 4 {P< 0,05) (hinh 2).
L6 1 L6 2 Lo 3 L6 4 Lo 5 L6 6 Ld 7
L61 L6 2 L6 3 L6 4 L o 5 L6 6 U6 7 Ham lugng MOS bo sung (%) Hinh 2. Ham lugng protein (% TL T) ciia ea
khoang co nemo a cac 16 thf nghiem Loi ddi chirng. Lo 2: 0.05'yo. L6 3 0.10%,L64-
0.15%. Lo 5. 0.20%. Ld 6- 0,25% Lo ?• 0.3%
So heu trinh hdy Id gid tri trung binh ± sai so. So li^u cd cdc chic cdi khdc nhau the hien sai khdc co y
nghia thdng ke (P <0.05)
Anh huong cua oligosaccharide hd sung ...
Mat d^ te bao mau cua ca nemo Vao tuan thu 10 o tSt ca cac nghiem thirc, mat do te bao mau dat gia tri cao nhat (26,22 xio* ± 2,80x10'tb/mrp^ tai nghiem thiic b6 sung 0,05 % MOS (i6 2), ke dkn la nghiem thiic b6 sung 0,25%
MOS (16 6) va 0,10% MOS (16 3) dat 2i,54xl0^±
3,65x10'tb/mm^ va 20,73xip^± 7,88x10'tb/mm^
mat dp te bao mau ca thip nhat (11,02x10' ± 2,00x|0'^ tb/mm^) a nghiem thirc 0,15% MOS (16 4). Te bao mau sai khac c6 y nghia giiia 16 cho an b6 sung 0,05% MOS (16 2) so voi 16 d6i chiing 0,0 % MOS va 16 cho an thuc an c6 bo sung 0,15%
MOS (16 4) (P < 0,05) nhung khong co s[i khac biet CO y nghia giira voi cac nghiem thirc b6 sung 0,1 %, 0,20%; 0,25% va 0,30% MOS (P > 0,05) (hinh 3).
Lo 1 16 2 Lo 3 Lo 4 Lo 5 L6 6 L6 7 Ham \{ftjr\g MOS {%) b i sung
Hinh 3. Mat do te bao mau ca nemo (tb X1 OVmm^) o eac 16 b6 sung MOS khac nhau Ld 1: ddi chicng Lo 2: 0.05%, Lo 3 0.10%, Lo 4:
0.15% Lo 5: 0.20% Lo 6 0.25%, Ld 7: 0.3%
So lieu trinh bdy la gid tri trung binh ± sai so. So lieu CO cdc chic cdi khdc nhau the hien sai khdc co y
nghTa thdng ke (P <0.05)
THAO LUAN
Be mat thanh rupt la noi hap thu chat dinh duong va trao d6i nang lugng va vat chat quan trong ciia co the. Dien tich be mat rugt Ion va khoe manh giup qua trinh trao doi chat hieu qua hon, lam giam nang luong tieu hoa (Sweetman va cs.. 2008). Ngoai ra, thanh rugt la noi cac vi sinh vat gay benh de dang xam nhap vao eo the vat chu Tiianh rugt khoe manh, voi he yi sinh vat CO lgi se lam tang hieu qua trao doi dinh duong va siic khang ciia vat chii. Nhieu nghien ciru cho thay hinh thai rugt ciia ea ciing thay doi khi ca bi nhiem vi khuan gay benh (Ringo
D6 Him Hoang, Hoang Diic Lu,.
va cs., 2007; Ringo va cs., 2004). Ty le giua chu vi ben trong va ben ngoai 6ng tieu hoa cita ea la mgt trong nhiing chi tieu dugc sir dung pho bien de danh gia hinh thai cua rugt ca va giap xae [5-8, 18].
Ket qua nghien cim eiia chung toi cho thay CO sir anh huong ro ret ciia ham lugng MOS bo sung va hinh thai rugt eiia ea khoang c6 sau 10 tuan nu6i. Ket qua nay cdng phii hgp voi eac ket qua nghien ciiu tren eae loai ca khac cho thay chuvl thanh rugt cua vat nu6i cung tang ro ret khi b6 sung MOS vao thiic an cua chiing [6- 8]. Ket qua de tai eo so nam 2012 cijng eho thay ca khoang c6 cho an MOS ciing c6 ty le ehu vi thanh rupt tang rat nhieu so voi 16 doi chiing (Kien va cs 2012).
Ham lugng protein trong ea la mpt trong nhiJng ehi thi quan trpng danh gia dieu kien dinh dudng ya siic khoe cua ea [13]. Dinh duang can bang giup ca sinh truong nhanh va ham lugng protein trong co ciing cao han. Ket qua phan tich ham^ lugng protein trong co ca nemo khi cho an b6 sung cac ham lugng MOS khong sai khac nhau co y nghTa thong ke. Ket qua nay tuong tu nhu cac ket qua nghien ciiu tren ca tam, Huso huso (Razeghi va cs., 2012), ca trap vang, Spams aurata (Dimitroglou va cs., 2010) [10], ca trap mom ngan, Diplodus puntazzo (Piccolo va cs., 2013). Cac nghien cuu nay eho thay viee bo sung MOS khong lam thay doi ham lugng protein cua ca. Tuy nhien, mgt so c6ng trinh khac ket luan rang ham lirgng protein trong co ca cho an MOS cao hon ban so voi ca khong cho an nhu tren ca chep canh [2], ca hoi [Oncorhynchus mykiss) va ea 10 phi lai (Oreochromis niloticiis x O. aureus) [9], ca chem (Dicenlrarchus labrax) (Torrecillas va es., 2007). D6i vai ca h6i Dai Tay Duong, Salmo sarla., khi b6 sung MOS vao thirc an thi ham lugng protein trong ca lai giam hon 5% (Grisdale-Helland va cs., 2008). Theo nhan dinh eua Grisdale-Helland va es. (2008) anh huong ciia MOS len ham lugng protein ciia ca CO the tuy thupc vao dac tinh ciia tiing loai.
Te bao mau dupc xem la mgt chi tieu quan trgng de danh gia sue khoe ciia ca nuoi [4]. Te bao mau cua vat nuoi co the bi anh huong tuy thuoc vao tinh trang dinh duong, sue khoe, bign dong cac yeu t6 moi truong va kich thuoc sinh vat Ket qua budc dau nghien CUTI anh huong ciia MOS tren ea khoang c6 nemo trong bao
cao nay cho thay MOS co the lam thay doi s6 lugng te bao mau ca nemo. Tuy nhien, so liju con nhieu dao dgng Ion. Miic do anh huong bdi ham lugng MOS bo sung qua cac m6c thoi gian kh6ng CO xu the ro rang. Nguyen nhan khac co the do kich thuoc ca co kich thiroc nho, the tich mau thu dugc rat it lam anh huong den sai s6 khi dpc the tfch mau thu dugc.
Ket qua nhien ciiu ciia chiing t6i cho th%
mat dp te bao mau ciia ca nemo chiu anh boi ham lupng MOS bo sung. Ket qua nay phu hop vai nhieu nghien ciiu tren cac loai ca khac nhu ca chep [1, 2], ca tam [3], ca tr6i (Andrews va cs., 2009). Tuy nhien, mpt s6 cong trinh nghien ciiu cho thay viec bo surig MOS khong anh huong den cac cac chi s6 huy6t hpc doi vai cac loai khac. Taati va cs. (2011) nhan dinh rang suthay doi cac chi s6 te bao mau co the chiu anh huong boi nhieu nhan to khac nhau nhu dac tinh eua timg loai, ham lugng MOS bo sungva thai gian eho an b6 sung.
Ket qua buoc dau cho thay bo sung MOS lam tang dien tfch be mat ciia thanh rugt ciia khoang e6 nemo sau 10 tuan nuoi, lam thay doi ham lupng te bao mau, tuy nhien ham lugng piotein trong co ca khong khac nhau co y nghTa khi bo sung cac n6ng do MOS khac nhau. Mac dil nghien cuu ciia chung toi buoc dau cho thay tinh tich cue khi bo sung MOS vao thiic an cua ca khoang c6 nemo, mpt so van de van can nghien cuu tiep nhu: anh huong cua MOS len ca nuoi o tinh trang sinh ly va benh tat, kha nang ch6ng chgi vai su thay d6i cua cac yeu to moi Iruang va cac sinh vat gay benh. Nhin chung nen bo sung 0,15% mannan oligosaccharide vao thirc an cho ca khoang co giai doan con non (~2 cm).
Loi cam ffn: Ket qua nghien ciiu la mgt phan ciia de tai nghien ciru anh huong MOS len ci khoang e6 nemo, tai Vien Hai duong hpc. Cac tac gia xin ehan thanh cam an su giiip da ciia lanh dao Vien va nhirng y kien dong gop quy bau cLia cac dong nghiep de hoan chinh bao cao nay.
TAI LIEU THAM KHAO
I. Akrami. /?., Chttsaz. H, Hezarjaribi, A. and Ziaei, R., 2012a. Effect of Dietary Mannan Oligosaccharide (MOS) on Growth
Performance and Immune Response of Gibel Carp Juveniles {Carassiiis auratus gibelio). J Vet Adv. 2, 507-513.
2. Akrami, R., Mansour, M. R., Chitsaz, H., Ziaei. R. and Ahmadi, Z, 2012b. Effect of Dietary Mannan Oligosaccharide on Growth Performance, Survival, Body Composition and Some Hematological Parameters of Carp Juvenile (Cyprinus Carpio). J. Anim. Sci. Adv. 2, 879-885.
3. Akrami, R., Razeghi Mansour, M., Ghobadi, S., Ahmadifar, E., Shaker Khoshroudl, M. and Moghimi Haji. M.S..
2013. Effect of prebiotic mannan oligosaccharide on hematological and blood serum biochemical parameters of cultured juvenile great sturgeon (Huso huso Linnaeus, 1754). Journal of Applied Ichthyology, doi: 10.111 l/jai.l2245.
4. Blaxhall, P. C and Daisley. K. W., 1973.
Routine haematological methods for use with fish blood. Journal of Fish Biology. 5, 771-781.
5. Daniels, C. L., Merrifield. D. L, Boolhroyd, D. P., Davies. S ' J., Factor. J R and Arnold. K. E.. 2010. Effect of dietary Bacillus spp. and mannan oligosaccharides (MOS) on European lobster {Homarus gammarus L.) larvae growth performance, gut morphology and gut mierobiota.
Aquaculture. 304, 49-57.
6. Dimitroglou. A.. Davies, S. and Sweetman, J., 2008. The effect of dietary mannan oligosaccharides on the intestinal histology of rainbow trout (Oncorhynchus mykiss) Comparative Biochemistry and Physiology, Part A. 150, Abstract.
7. Dimitroglou, A.. Moale. R., Janssens, T, Spring, P., Sweetman, J. W. and Davies, S.
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Improves Health Status of the D i g e s t i v e
EFFECTS OF DIETARY MANNAN OLIGOSACCHARIDE ON MUSCLE PROTEIN, INTESTINAL MORPHOLOGY AND BLOOD CELL COUNT
OF CLOWNFISH, AMPHIPRION OCELLARIS
Do H u u H o a n g , H o a n g D u e L u , P h a m X u a n K y , D a n g T r a n T u T r a m , N g u y e n Thi Kim B i c h , H o S o n L a m , T r a n V a n H u y n h , D a o \ ' i e t H a , N g u y e n T h u H o n g , P h a n Bao Vi
Institute of O c e a n o g r a p h y , V A S T
ABSTRACT: Clownfish, Amphiprion ocellaris (2 4 cm) were cultured in recirculation system, volume of 50 L and density of 20 fish per tank. Six levels of mannan oligosacharide (MOS), including 0 05% 0 10% 0 15% 0 20%. 0.25%. and 0.30% were supplemented to the control diet and fed to the fishfor 10 weeks. Collected data included ratio of internal and external intestinal perimeter of the gut (PR), muscle protein content and total blood cell count. Result showed that the PR was highest in the fish fed by 0.10%, and 0 15% MOS and lowest in two groups of fish fed by control and 0 3% MOS in their diet (P < 0.05). After 4 weeks of diet feeding, protein content m the fish among the treatments ranged from 12.85%P to 15 53%P Those values seemed lo increase at week 10 in compared to u'eek 4, however; there was no significant difference in protein content among the treatments al either week 4 or week 10 measurements. Total blood cell count offish at week 10 were highest in the group offish fed on 0.05% MOS (26.22^I(f ± 2.80-^l(f cells/mn^), followed by the groups of fish fed 0 25% and 0.1% with blood count being 2I.54^1(f ± S.eS-"!^
cells/mm^ and 20.73 >^ I (f ± 7.88 ^ICi cells/mm'. Respectively the lowest blood cell count was in the fish fed by 0.15% MOS (II 02^1(f ± 2.00 xl(^ cells /mm^j. There was a stalislically significant difference between blood cell count offish fed by 0.05 % MOS in compared ta fish fed by control diet and 015% MOS (P
< 0.05).
Keywords: Clownfish. Amphiprion ocellaris. mannan oligosaccharide, gui morphology, protein, blood cell
