HQI NGHI KHOA HOC T O A N Q U O C V ^ SINH THAI V A TAI N G U Y £ N SINH VAT LAN THLf BA
UTVG DVNG CAC CHlTONG TRINH MAY TINH VA DtT LIEU NGAN HANG GEN DE TIM HIEU M 6 I QUAN HE GAN GUI CO THE CUA CHI SAM
NAM - CYCLEA V6fl CAC CHI KHAC THUQC HQ TIET DE - MENISPERMACEAE
VU TIEN CHINH, BUI HONG QUANG, TRAN THE BACH Vi^n Sinh thdi vd Tdi nguyen sinh vdt JOONGKU LEE Vi?n Nghien ciru sinh hgc vd Cong ngh? sinh hgc Hdn Quoc Ngay nay, ciing vdi tien bp cua khoa hpc va cdng nghf, vifc tim hiiu mdi quan hf gan gui cua cic taxon dang ngay cing phd bien. Cic die dilm la cac nucleotide da cung cap mpt_lugng thdng tm khdng lo, cic chuong frmh miy tinh se la cdng cy quan trpng khdng the thieu de phan tich du lifu dd. Q Vift Nam, phuong phip kit hgp giira ihig dyng cac chuong frinh iniy tmh yi dvt lifu ngin hing gen dl tim hiiu moi quan hf gin giii cd thl cua chi Cyclea vai cac chi khac thudc hp Menispermaceae chua dugc nghien cuu. Chinh vi vay, chiing tdi da chpn dl^tai "Ung dyng cac chuong trmh miy tmh va dir lifu ngan hang gen d l tim hieu mdi quan hf gin giii cd thl cua chi Sam nam - Cyclea vdi cac chi khic thupc hp Tilt de - Menispermaceae".
L PHU^ONG PHAP NGHIEN CUtJ
Tap hgp thdng tin trinh ty gen thu dugc cua cic taxon thupc hp Tilt de (Menispermaceae):
Thu thap dir lifu trinh ty nucleotide cua gen matK (day la gen rit quan trgng dugc ling dung trong tim hiiu moi quan hf gin gui cd thl va moi quan he phit sinh chimg loai cua thye vat cd hoa) tren trang http://www.ncbi.nlm.nih.gov, dimg chucmg trinh CLC de thu thap dir lifu nhanh tu Intemet. Gidng hang frinh ty bing phan mem ClustalX2.
LTng dyng chuong trinh miy tinh PAUP (phuorng phip khoing each va phucmg phip Parsimony) v i chuong frinh Mr Bayes dk tim hiiu moi quan hf gan giii cd the cua cic taxon.
iTng dyng chuong trinh miy tinh TreeView va Mega 4.0 d l thl hifn cay quan hf gan giii cd the.
n . KET QUA NGHIEN CU*U
1. Danh sich cac loai l\ra chpn nghien ciru v^ ma so tren ngan hang gen STT
1 2 3 4 5 6 7 8 9 10 11 12 13
Lo^i Akebia quinata Albertisia laurifolia Anamirta cocculus Arcangelisia gusanlung Aspidocarya uvifera
Cissampelos pareira Cocculus laurifolius Cocculus orbiculatus Cyclea barbala Cyclea hypoglauca Cyclea polypelala Cyclea tonkinensis Cyclea waltii
M2 so tren ngan h&ng gen EF143878
EF143849 EF143856 EF143852 EF143853 DQ401349 EF143859 AB069852 EF143861 EF143862 EF143863 EF143864 EF143865
Chii thich Chi khong co d Vift Nam Chi CO d Vift Nam Chi CO d Vift Nam Chi cd d Vift Nam Chi khong co d Vift Nam Chi c6 d Vift Nam Chi cd d Vift Nam Chi c6 d Vift Nam Chi cd d Vift Nam Chi CO d Viet Nam Chi c6 d Vift Nam Chi CO d Vift Nam Chi CO d Vift Nam
46
HOI NGHI KHOA HOC TOAN QUOC V £ SINH THAI VA TAI NGUYEN SINH VAT LAN THU BA
14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32
Diploclisia ajfinis Diploclisia glaucescens Hypserpa nitida Pachygone valida Parabaena sagittata Pericampylus glaucus Pycnarrhena lucida Sargentodoxa cuneata Sinofranchetia chinensis Sinomenium acutum Stephania brachyandra Stephania cephalantha Stephania chingtungensis Stephania delavayi Stephania elegans Stephania longa Stephania succifera Stephania tetrandra Tinospora sinensis
EF143866 EF143867 EF143868 EF143850 EF143854 EF143869 EF143851 DQ401351 EF143879 EF143870 • EF143871 EF143872 EF 143873 AF542589 EF143874 EF143875 EF143876 EF143877 EF143855
Chi CO d Viet Nam Chi CO d Viet Nam Chi CO d Viet Nam Chi CO d Viet Nam Chi CO d Vift Nam Chi CO d Viet Nam Chi CO d Viet Nam Chi CO d Vift Nam Chi khong co d Viet Nam Chi khong co d Viet Nam Chi CO d Viet Nam Chi CO d Viet Nam Chi CO d Viet Nam Chi CO d Viet Nam Chi CO d Viet Nam Chi CO d Viet Nam Chi CO d Vift Nam Chi CO d Viet Nam Chi CO d Vift Nam
2. Ung dyng chirong trinh PAUP
2.1. Phuong phap khoang each: Sau khi nhap dir lifu vao chuong trinh Paup, ket qua the hifn bang chuong trinh TreeView qua hinh 1: So dd chi ra cic loai ciia chi Cyclea gan giii vdi chi Cissampelos. Hai chi gdp thanh nhdm cd quan hf gin gui vdi chi Stephania.
S r j i r t o c a u :un4«j Akiiu i^ui-iitj SinohxiOiilia c h i r f i i i * Sinoi'injjnr icuum Amr-mt ;eccjlj«
M i n o l t a guiirjuig TnDi93<3 lintniit AiftOactr/i UvifH) PitlbtrM lif KAt Difitcdiii'i giiLCKCtnt Ci^cditi} itTfiii HyptNTi n u l l
Al u n i t 13 liurtfdil Pycnwrhtna \u:A»
Ccccului IU=KUll(jt Ccccului \KlM\tt Pichyscnf «td»
Ciii>Tp«<ii pirwr*
Cychu barbali C/cl4((n(/F«til) C | c l « a h ^ ? c ^ j c i
C'/dUl3Hirf1MtJ|
31 epfian.! longa St-^^sndlftnncki
SUftitn a lucciferI Sl9fttn^ ctfttttiOv Slt^hm ( chnjtunBtnui
47
HOI NGHI KHOA HOC T O A N Q U O C V £ SINH THAI V A TAI N G U Y £ N SINH VAT LAN THU' BA
2.2. Phuong phap Parsimony: Ket qui the hif n bing chuong trinh TreeView va Mega qi hhih 2: so dd chi ra cic loai cua chi Cyclea gin giii vdi chi Cissampelos. Hai chi gpp thai nhdm cd quan he gin gui vdi chi Stephania.
Siir£;<^iitodn\u c u n c a i a A k c h U i (.{uiniitit S n i o t r n n c h e t i i i c h u t c r u i b A i i a n i i r t u coc<:ulut<
Arx:iirt^i^]ijiKi tjusunhinjjt 'Cino.'^porji «ini^i.sln A - s p k l o c i i r v u u v i f e r a I ' a r u b u c i u i s u g i l l u t u S i n o i i i c n i i u i i a c u l i u i i Dij:>ktclis>i» KljtiJceMctais D i p l o c l l s i u ufTbiis I ly[>»i;:rpfi n i l i t l a F c r i c - u i i i p y l u s {IUIUCUN C t t c c i i l i i s o r b i c i i h i t i i s C o c c u l u s lHuriri>liuA ^ P u c l i y ^ o n c vulidci A t b c n i s u i lituriri)ltti F>'\:nurrhciiii hickJu C!tH!ii:tnipt:liis p n r c b i t t'yclc-a bori>ntn t,"yck.'ii p o l y p c u i U i C y c l e a h y p n t i U i u c i i C y c l e a vvatlii C y c l e * ! toiikiiieiLsbi S t c p l i n n i u i i u c c H e r u S t e p h i i n U i c h i n ^ t i u i g e i i s i s S t c p h i u i i t i b i - u f l i y i u u l r « Slcphuniia c e p h n l u n i h i i S i e p l t u n i u t l t f t i v i i y i Sturpluiriia UHmiidra S t e p h a n i a k>ny4i S t e p h i i i i i i t cleiziinh
Hinh 2: Quan hf gan gui co the cua 30 loai, 17 chi cua hp Menispermaceae va 2 loai, 2 chi cua Lardizabalaceae tren ca sd irng dung phvong phap Parsimony
cua chuong trinh PAUP
23, Chutmg trinh Mr Bayes: Ket qua the hi?n Mng chuang trinh TreeView qua hinh 3: sa do chi n cac Ioai cua chi Cyclea gan gui \6i chi Cissampelos. Hai chi gpp thanh nhom co quan hf gan gui voi chi Stephania.
Sarpertodort cineitd
' Sindf«nchrta chinensis i — r ^ ^/lamirt* coccLkiS ' — ' ~ Arcingefiafl gusartuig 1 Tinospora s;r»nsr5
~ 1 ' f - H b A s p i d o c a ^ f l w t e i ' Parabaena sa^^aaU
Stnomeniim ioisn
-b'..
^ ^ - ^ .
Pef^UTC'ytjs giauQS r r p j j Di[>odsiftflaLCesc«ns ' — — Dipoctsia eliris
^—'— Pycnarhena lucida J C o c o l j s oTOicjtaftis 1 ^ f - T T v , Coc:iijslainloSus ' — ^ Pachygone val da
Cissampekn pareiia C^c«a bait)ala C)'C«i pot>p«tila C)fr.«a fiyocflbuca Cyc«a watUi C « : « tonkinerss Siephtnii s t c c f w i _ i — J P I J Slaphfiria c*p^»aJarth«
' Siaphania delavar' r ^ j i ^ SlephaTiachngtLn(«risi ' Stepha-na bractrfandri J — — — aephama tejanaa
1 — J o u Siep'*'**fcnga aephamt eegans
Hinh 3: Quan hf gan gui c6 the cua 30 loai, 17 chi cua hg Menispermaceae va 2 loai, 2 chi cua Lardizabalaceae tren ca sd irng dung chuong trinh Mr Bayes.
48
HOI NGHI KHOA HOC TOAN Q U O C V £ SINH THAI V A TAI N G U Y £ N SINH VAT LAN THU BA
III. KET LUAN
Ca 3 so dd deu chi ra chi Cyclea gan giii vdi chi Cissampelos. Hai chi gdp thanh nhdm cd quan hf gin gui vdi chi Stephania.
TAI LIEU THAM KHAO
1. Nguyen Tien Ban, 2003: Danh luc cic loai thuc vat Vift Nam, 2: 140-151. NXB Ndng nghifp, HaNpi.
2. Vu Tien Chinh, Vu Xuan Phuong, 2004: Tap chi Sinh hoc 26 (4A): 46-48.
3. Vu Tien Chinh, Vu Xuan Phuong, Tran Ninh, 2005: Nhung vin de nghien cuu co bin.
Trong khoa hoc sy sdng: 84-87. NXB. Khoa hpc va ky thuat.
4. Knudsen B. et al., 2008: CLC Sequence Viewer.
5. Larkin, M.A. et al., 2007: Clustal W and Clustal X version 2.0. Biomformatics, 23:2947- 2948.
6. Pham Hoang H9, 1991: Cay cd Vift Nam, 1(1), tr. 417-419, Montreal.
7. RonquistF., John P . H . «& Paul IVI., 2005: MrBayes 3.1 program.
8. Swofford, D. L., 1998: PAUP*. Phylogenetic Analysis Using Parsimony (and Other Methodsj, Version 4. Sinauer. Associates, Sunderland, Massachusetts. (PAUP'" version 4.0 blO computer program).
9. Tamura K. et al., 2007: MEGA4, Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24:1596-1599.
10. http://www.ncbi.nlm.nih.eov
USE COMPUTER PROGRAMMES AND GEIVBANKTO DETERiVUNE PHYLOGENfETIC RELATIONSHIPS O F GENUS CYCLEA AND O T H E R GENERA
O F MENISPERiVIACEAE IN VIETNAM
VU TIEN CHINH, BUI HONG QUANG, TRAN THE BACH -•* ^ SUMMARY "• . "
We have used modem computer programmes such as PAUP, Mr. Bayes, TreeView, Mega, ClustalX2, CLC Sequence Viewer to find relationship of genus Cyclea and other genera of Menispermaceae in Vietnam. We collected information of 32 species in Genbank from the website: http://www.ncbi.nlm.nih.gov and CLC Sequence Viewer programme. We use PAUP and Mr. Bayes to find phylogenetic relationships among taxa, TreeView and Mega to present diagrams showing relationship. Using distance and parsimony methods in PAUP program, Cyclea & Cissampelos formed a group related to Stephania. Representative genera were selected: Akebia, Albertisia, Anamirta, Arcangelisia, Aspidocarya, Cissampelos, Cocculus, Cyclea, Diploclisia, Hypserpa, Pachygone, Parabaena, Pericampylus, Pycnarrhena.
Sargentodoxa, Sinofranchetia, Sinomenium, Stephania, Tinospora. Important results of methods showed that Cyclea related to Cissampelos. The results of this paper will contribute to further studies on taxonomy of Menispermaceae m Vietnam.
49
