KHOA HOC CbNG NGHl
IMHAIM DAIMG CAC LOAI CLIA XAIMH [Scyiia s p ) d VIET IMAIVI B A I M G G I A I TRIIMH TIJ ADIM TY THE VIJIMG
GIEIM COI
Thii Thanh Bmh\ Nguydn Van Vidt', Christopher Austin^, Trin Thi Trang^
TOMTAT
Cua xanh la loai co gia tri kinh te cao va dang trd thanh dcii lucrng nuoi quan trong cr cac viing ven bien Viet Nam. Hang nam khoang 100 Irieu cua gicing ducrc san xuat tir 150 trai gidng. Mac dii cua xanh ducrc nuoi lau nam d Viet Nam, nhimg nhung nghien ciai vc nhan dang cac loai cua xanh van con han ch^ do chu yeu dua vao hinh thai ben ngoai. Nghien ciru nay trinh bay ket qua nhan dang cua xanh dua tren trinh tu ADN a ty the. 33 miu cua xanh duoc thu tir 10 tinh ven bien cV Viet Nam va Uc ducx- giai ma trinh tu ADN viing gien cytochrome oxidase subunit I (COI - ciu triic sieu phan tir men oxy hoa sic tri t^ bao). Trinh tu ADN ciia cua xanh \'iet Nam cung ducrc so sanh vcri trinh tu ADN cua cua xanh a cac nucrc nhu Trung Qucrc, Uc va Indonexia. Ket qua nghien ciru cho thay vimg gien COI rat hiru hieu trong viec nhan dang cac loai cua xanh. Viet Nam co 4 loai cua xanh: Siyi/a pa/Tuiiamosian, S. serrata, S. olivacea va 5. tranquebaria. Trong do loai S. paraiuamosia bit g«ip phd bien nhit. Xc?t ve khoang each di truyen, loai S. paramamosian gan gui voi 2 loai 5. tranquebaria va .s'. serrata, khac xa vcM loai 5. olivacea. Ket qua nghien ciru gop phan quan trong trong vice xay di.mg he thong phan loai, dinh hucrng lua chon vat lieu cho chcpn gidng va bao ton cac loai cua xanh d \'iet Nam.
Tir khoa: Cua xanh, phan loai, S. olivacea, S. paramamosian, S. serrata, S. tranquebaria.
i. DAT VAN BE hgc da cd g i n g dua vio c i c chi tidu hinh thii nhir Cua xanh (Scylla sp) l i ddi tugng nudi thuy s i n hinh dang v i chidu cao cua gai thiiy trin; gai cd tay CO gia tri xuit khau v i gia tri kinh te cao d nhieu v i gai trudc cua cing; binh dang v i kich thudc ciia nuoc trong khu vuc Dong Nam A. Cua co ham lugng gai giao cau de phan loai cua (Thii Thanh Duong vi mo thap, phong phii k h o i n g chat v i c i c vitamin. Viet ctv, 2003). Nhung theo Ling- Bo (2006) hinh thii cua Nam da cho cua smh s i n thanh cdng tir nam 2000, cua r i t dd bidn ddi theo moi trudng sdng.
cho den nay s i n lugng cua gidng n g i y mgt tang cao. Hidn nay vide sir dung chi thi phan tir (markers) Theo Nguydn Ca Thach (2003), h i n g nam tidn 100 trong p h i n loai smh vat rat phd bien, dac bidt l i trieu cua gidng dugc s i n x u i t tir hon 150 trai s i n nhirng loii co hinh thii thay ddi theo moi trudng cao xuat gidng. Cua ducic nuoi d k h i p c i c tinh ven bien v i gianh gidi giira c i c loai mong manh (Liu v i Viet Nam, dac bidt d c i c viing cira song chau thd Cordes, 2004). Giai trinh tu ADN c i c viing gien d ty song Hdng v i song Md Kdng. Didn tich nuoi cua the cho ket qua nhanh, chinh x i c ; thu mau khong nam 1999 la 5.813 ha, den nam 2005 tang Idn 25.355 gay hai den dgng vat v i r i t huu hidu trong phan loai ha, nang suit dat 1000 k g / h a / v u . San lugng cua sinh vat. Mgt sd nghidn cuu phan loai ve cua dua vio xanh dugc xuat khau nam 2004 l i 6.000 tan. thu ve sd hdu hinh thii v i di truyen phan tir da dugc cong kim ngach ban 25 tridu USD (Luidner, 2005). bd. Keenan vi ctv (1998) dua vio sd lieu hinh thii v i Mac dii cua xanh ducrc nuoi d Viet Nam nhidu Allozyme da chia gidng cua xanh (Scylla) t h i n h bdn nam. nhung nghien cuu vd n h i n dang cua xanh vin loii: Scylla serata, S. olivacea, S. tranquebarica va S.
han che v i cdn nhieu tianh cai vd sd lugng loii v i paramanosain. Lmg-Bo v i ddng t i c gia (2006) phan loai dugc nuoi phd bien hidn nay. Vide dua ra c i c chi tich giai trinh tu ADN ty tiie vung gien COI ciia cua tidu hinh thii de l i m can cu cho p h i n loai n h i m t i c h xanh d Trung Qudc v i cho r i n g 5. paramanosain l i rieng cic loii trong gidng cua xanh het sire kho loii k h i c bidt vdi 3 loii: 5. olivace, S. tranquebarica, khan, phirc tap v i k h d n g ro r i n g , dac bidt d giai doan 5. serrata v i dugc nuoi phd bidn d nude nay.
cua chua trudng t h i n h . Tuy nhien c i e n h i p h i n loai Klinbunga vi ctv (2000) su dung RAPD da dua ra marker dd p h i n bidt cho ba loii eua Scylla serata, S.
oceanica, S. tianquebaricabThkiTan.
^ Tnrong Cao dang Thuy san Trong nhtmg nam gin diy, phuong phip niy da Khoa Khoa hpc, Tmdng Dai hgc Monash, phan hi?u ^.^ ^ . ^ ^ ^ ^ ^^ j ^ ^ ^^^ ^^^ ^^.. ^ . bunway, Malaysia . t- . o x-
Dai hc?c Su pham Ha Ngi
NONG NGHIEP VA PHAT TRIEN NONG THON KY 1 THANG 4/2012 77
thiiy san d Viet Nam. Ngo Si Van va Tliai Thanh Binh (2005) da hoan thidn dinh loai c i rci tdng trudng va ca trau tidn vua bing each giai ma 16S rRNA trdn ADN ty the va RAGl tidn ADN cua nhin td bio. Tliii Thanh Binh vi cdng tac vidn (2004, 2006) phin bidt ducpc su khic nhau giOra c.i chep tring VicM Nam va cic dcmg ca chep triMi Uid gicri bang phucmg phip giai trmh ADN d ty thd viing gien 1 )-Ix)op, ATP6 vi ATP8. Tliii Ilianh Binh iv/ ctv (2007) da i)lian loai cic loii c i ti"ong ho c i chep dua ticii giai ma trinh lu ADN cua ty tlic vimg gien 16S ArRN, D-Loop vi Cytochrome (sic td td ban) b. Tuy nhidn chua co nghidn cim nhan dang cic loii cua xanh bang chi thi phan tir. Trong nghidn cim nay cac loii cua xanh cV Viet Nam ducrc nhan dang bing gi;ii trinh tu ADN d ty the va duge so sanh vcri cic loai cua xanh d mgt so nucrc trdn tlid gicri nhu Uc, Trung QUCK, Indcindxia nhim gdp phin lira chgn vat lieu ban dau cho chgn gidng vi bao tdn gidng cua xmih cua Vii-t Nam.
L VAT UEU VA PHUONG PHAP NGHEN CUU
320 mau cua xanh dugc thu tir 10 tinh d Viet Nam (30 mau /tinh) vi thinh phd Darwin - Uc (20 mau) phuc vu cho nghien cim. O mdi mgt tinh 3 mau cua xanh duge lua chgn de giii trinh tu ADN. Dia diem thu mau va ky bidu mau dugc trinh biy d bing 1. Mau eua dugc bio quin trong edn 90%. Gidng cua xanh duge phan bidt dua theo phuang phip cua Keenan vi ctv (1998). Tdng sd ADN dugc tich chidt theo Bing 1. Cic quin thd
phuang phip cua Crandall vi ctv (1999). Vimg gien Cytochrome oxidase subunit I (COI - cau tnic sieu phin tii men oxy hda sic td td bao) d ty the dupe khudch dai bang cap mdi mtillO: 5 T TGA TTTTTT (iGT CAT CCA GAAGT 3' (Roehrdanz, 1993) va C/N2769: 5' TT AAG TCC TAGAAA ATG TTG RGG GA 3' (Gopuneko et al, 1999). Thinh phin cho 25 y.1 phan img PCR gc'm: 1 X buffer, 2 mM dNTPs, 1,5 niM MgCl^, 0,5 pM mdi, 0,1 units Tac/polymeraza va khoang 200 ng ADN mau. Kliudch dai doan COI cua ly the cua bang may PCR - Corbett PC 960 vcri chu ky nhiit 94"C 3 phut, 34 chu ky vdi 94"C 30 giay, 50"C 20 giay, 72"C 30 giay v i cudi cimg 72"C 5 phiil.
S;in pham PCR dugc kiem tra bing phuong phip chin di vcri marker DNA/Hac III. San pham PCR dugc lim sach bing bd kit ciia Hang QIAGEN.
Trinh lu ADN ty thd (mtADN) eua cua dugc giai ma trdn miy ABI/3130XL. Trinh tu ADN ty the cua cic loii cua xanh Trung Qudc, Inddndxia vi Uc (co sin tiong ngin hing gien) dugc sir dung de so sinh vcri cua xanh Viet Nam bing phin mem BioEdit
(Hall, 1999). Trinh tu ADN cua ghe xanh cd quan he gin gOi vcri cua xanh dugc su dung so sinh gidng nhu nhom ddi chimg. He sd da dang haplotyp Otieu don) ciia cua xanh dugc tinh dua trdn phin mem DNASP (Roza et al, 2004). Mdi lidn be giiia cic loai cua xanh ducrc xay dung trdn phin mem vi MEGA
(Jamura e/'a/., 2 0 n ) . eua xanh dugc thu miu STT
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
Quin the Hii Phdng
Quing Ninh Thii Binh Ninh Binh Quang Ngai Khinh Hoi CiMau Bac Lidu Sdc Trang Binh Dmh Darwin 5. serrata
S. paramamosian S. olivacea S. tranquebarica
Portunus trituberculatus
Ky hidu mau HP QN TB ND QN KH CM BL BT BD DA SSE SP OL STR GHE
Dia diem thu mau Kidn Thuy - Dd Scm - Hii Phdng Hoing Tin - Yen Hung - Quing Nmh Dong Minh - Tidn Hai - T h i i Binh Kim Tin - Kim Son - Ninh Binh Phd Trach - Due Phd - Quing Ngai Ninh Lgc - Van Nmh - Khinh Hoi Mui Dit - Nggc Hien - Ci Mau Ginh Hio - Ddng Hii - Bac Lidu Tin Phii - Sdc Trang
Qui Nhon - Binh Dinh
Cang Danvm - Darwin - Austialia Queensland - Austialia
Trung Qudc Bali - Inddndxia Bali - Inddndxia
Hdng Kdng - Trung Qudc
Sd mau giii trinh tu
3 3 3 3 3 3 3 3 3 3 3 AF097002*
AY750930*
AY373355*
AY373353* _ AP082732* _ ' Sdtruy cap ngin hing gien
KHOA HOC CbNG N G H l
in. KET QUA VA THAO LUAN
1. Da dang di truydn vi nhan dang ciia cua xanh Tdng sd 490 bp viing gien COI dugc giai ma cho timg mau eua thu tir 10 tinh d Viet Nam. Khi so sinh 38 trinh tir ADN (33 timh tu ADN cua xanh ciia nghien cuu niy vi 5 tiinh tu ADN cua xanh ducrc lay til ngan hing gien) ed 106 vi tri sai khic va 24 haplotyp (Kieu don bdi) dugc phat hidn. Cac haplotyp khic nhau tir 5 - 12 bp. He sd da dang ciia haplotyp li 0,964 ± 0,007. Chi tinh ridng cua xanh Viet Nam cd 61 vi tri bidn di, 17 haplotyp va he da dang cua haplotyp li 0,943. So vdi cua xanh Trung Qudc he sd bidn di cua cua xanh Viet Nam cao gip 1,2 lan (Lin Bo, 2006). So sinh trinh tix ADN cua cua xanh d Viet Nam vdi cic loai cua xanh trdn the gicri bing bidn phip sir dung phin mem BLAST cho thiy gidng nhau tir 98 - 99%.
Cay phi he Neighbour joining (NJ kdt ndi dong loai) vi Minimum Evolution (ME tidn boa thap nhit) dugc xiy dung trdn sd lieu trinh tu ADN ciia 30 miu cua xanh d Viet Nam, 4 miu eua xanh cua Uc. 2 mau eua xanh eua Trung Qudc vi 2 mau cua xanh cua Inddndxia bieu didn mdi quan be giira cac loai cua. Ci bai ciy phi he cho ket qui gidng nhau vi chia cic loii eua thinh 4 nhdm ridng bidt vdi tri sd ung hg cao (tir 95-99%). Cic nhdm niy khic nhau tir 2,1-3,62% (Hmh 1).
Nhdm tiiii nhit gdm 23 mau cua tiong dd 21 mau cua Viet Nam vi 2 mau cua Trung Qudc; hai miu niy da dugc xic dinh li loai S. paramamosian, do do nhom thu nhit dugc nhan dang li loii 5.
paramamosian, nhom thir hai li loii 5. tranquebarica vi trong nhdm niy cd miu cua eua Inddndxia (STR) da duge xic dinh li S. tianquebariea. Nhdm 3 duge xac dinh li loii 5. serrata, bao gdm cua d Khinh Hda, Binh Dinh vi toin bd mau cua d Uc. Cua xanh cua Uc chu ydu li loii 5. serrata. Nhdm thir 4 gdm mau cua d Quing Ninh, Ci Mau vi 2 man cua d Indonexia. Cic miu cua d Inddndxia da dugc dinh danh li S. olivace, do dd nhdm niy thugc loii 5.
olivace. Qua kdt qui nghidn cim cho thay Viet Nam : CO 4 loii eua xanh. Trong 30 miu cua xanh d Viet J Nam duge phin tich cd den 21 mau duge xic dinh li ', loai 5. paramamosian, do dd loai cua xanh 5.
. paramamosian duge xic dinh la loii phan bd pho ' bien nhit d cic tinh ven bien Viet Nam hidn nay.
Theo Starobogatov (1972) loii cua xanh d Vinh Bic bd Vidt Nam li loii S. oceaniea. Khi thu mau cua d ddng bang song Md Kong, nhi phin loai hgc Keenan vi ddng tic gia (1998) kdt luin loii cua xanh sdng d khu vue cira song Md Kong li loii cua 5.
paramaniosain. Theo Macintosh vi ddng tic gia (2002) viing ha luu sdng Md Kong cd hai 2 loii cua li .S". pramaniosain va S. olivace. Tuy nhidn hiu bet cic cong trinh nghidn cim vd phin loai cua chu yeu dua vio hinh thai bdn ngoii vi pham vi thu mau hep. Kdt qua nghic-n cim niy cho thay ba luu song Md Kong cd it nhai 3 loii: S. paramamosian, S. olivace vi S.
serrata. 6 Vinh Bic bg co 3 loii: 5. paramamosian, S.
tranquebarica vi 5. serrata.
99/99 98/95
73/78
99/99
^
95/99
CM2 BL1 KH3 GND TB3 TB2 ,BD3 I H ^
CM3 ST1 -NB2 iNBI I N B 3 - B L 3
BD2 ST2 BL2 ST3 SP2 if-l-P1
|TB1 I Q N 2 SP1 STR KHI I-P2
I—BDI
" ~ I K H 2 DAI DA2 DA3 SSE
99/99
A
S. paramamosian
J
0L1 • 0L2 QN1 CMl GHE
S. tranquebarica
S. serrata
S olivacea
0.02
Hmh 1. cay phi h0 Neighbour-joining dugc xSy dung tir vung gien COI ciia ty thd cua xanh Tri sd tin ciy cua cic nhdm dupe nit ra tir so sinh cic tiinh tu ADN vdi 1.000 lan lip; model Kimura 2- parameter Gii tii cua Neigh-joining a bin trin, Minimum Evolution a bin dudi. Ky hiiu mau eua xem a bing 1.
2. Khoang cich di truydn ciia cic loii cua xanh Khoang cich di truyen cua cie loii cua xanh Viet Nam duge tiinh biy d bang 2. Kdt qua nghidn
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 1 - THANG 4/2012 79
cim mdi quan hd (Khoang c i c h di truydn) giua c i c loai cua xanh d Viet Nam cho thay loai cua xanh S.
paramamosian cd mdi quan hd gan nhat vcVi loai S.
Tranquebariaca, tidp ddn la loai .S". serrata va k h i c xa vcri loai S. olivace (0.172). Ket qu.i nghien cim nay
B i n g 2. K h o i n g c i c h di truydn gi&a c i c loii cua Loai
S. paramamosimi S. seirata
S. ti-anquebarica S. oliv:icca
Portun us trituberculatus
cung triing hgp vb\ n h i n dinh cua Keenan vi ctv (1998) khi nghidn ciiu phan loai cua xanh d bien Hdng Hai T r u n g Qudc, Hdng Kong. Indondxia. Thai Lan bang bi^n p h i p sir d u n g tiinh tu ADN viing gien
1(>S ArRN.
d Vi^t Nam di;a tidn trinh t u ADN vimg gien COI
^V. jKiraniamosian 0.109
0,081 0,172 0,186
S. .serrata
0,091 0.1.58 0,170
S. tianquebarica
0,134 0,173
S. olivacea
0,214 IV. KET LUAN
Kdt qua nghidn ciai buc're dau cho thay viing gien COI (cau triic sidu phan tir men oxy hc)a s i c td id bio) rit hiru hidu trong vii-c- nhan dang cac loai cua xanh. V\c\ Nam co 4 loai cua xanh: S. seirata, S.
Tranquibarica, S. paiamariiosiari, va S. olivace. Ijojii cua 5. paramamosian dugc b i t gap nhidu n h i t .
Hidn nay nghd nuoi cua xanh dang p h i t tridn manh me, vide trung chuyen cua giiira c i c vimg nuoi anh hudng ddn da dang cua cua. Chat lugng gidng cua n g i y c i n g giam sut. Dd phuc vu cho bao tdn v i chgn gidng cua xanh c i n cd c i c nghidn ciru d i n h g i i da dang q u i n the cua xanh v i so s i n h da dang di truydn eiia cua xanh trong trai s i n x u i t v i ngoii tu nhidn.
TAI UEU THAM KHAO
1. T h i i Thanh Duong. T r i n Manh T u i n . Nguydn Kun Phuc. Nguyen Van Chung, Pham Dinh Trgng.
2003. Mgt sd loii g i i p x i c thudng gap d Viet Nam.
Trung tim Thdng tin Bd Thuy sin. Trang 81.
2. Crandall K. A., Fetzner J. W.. Lawler S. H..
Kmnersley M. and Austin C. M., 1999.
Phylogenetic relationships a m o n g the Australian and New Zealand genera of 6-eshwater crayfishes
(Decapoda: Parastaeidae). Austialian Journal of Zoology^: 199-214.
3. Gopuneko D., H u g h e s M. J., Keenan P. C , 1999. Mitochondrial DNA evidence for rapid colonisation tiie Indo - West Pacific by tiie mud crab Sylla serrata. Marine Biology \9^ 134: 227-233.
4. Hall T. A , 1999. BioEdit: a user fi-iendly biological sequence alignment editor and analysis program for Windows 9 5 / 9 8 / N T . Nuel Acids.
5JTO/7. 5 e r 4 1 : 9 5 - 9 8 .
5. Kec-nan C. P., Davie P F., Mann D. L , 1998. A revision of the g e n u s Scylla dc Haan. 1833. The Raffles Bulletin of Zoology 46:217 245.
6. Keenan C. P.. Davie P F & Mann D. L . 1998.
A revision of the g e n u s Scylla de Haan. 1833. The Raffles Bulletin of Zoology \^: 217-245.
7. Klinbunga S., Boonyapakdee A.. Pratoomchat B.. 2000. Genetic diversity and species diagnostic markers of mud crabs (Genus Scylldj in eastern Thailand determined by RAPD analysis. Mar.
Biotechnol. 2: 180-187.
8. Lindner B.. 2005. Impacts of mud crab hatchery technology in Vietnam. In: Impact Assessment Series Report No.36, p. 66. ACIAR, Austialia.
9. Un Bo M.. Feng Ying M.. Chun Yan M..
Zhen Guo G., 2006. Scylla paramaniosain (Eslampaton) the most common mud crab (Genus Scylla) in China: evidence from mtDNA. Aquaculture Research 37:1694 -1698.
10. Macintosh D. J., Overton J. L , Thu H. V. T , 2002. Confirmation of two common mud crab species (genus Scylla) in the mangrove ecosystem of tiie M e k o n g Delta, Vietiiam. Journal of Shellish Research 21:2^9-2^.
11. U u Z. J., Cordes F. J., 2004. DNA marker technologies and t h e u applications in aquaculture genetics. Aquaculture 238:1-37.
12. Macintosh D. J., Overton J. L , Thu H. V. T , 2002. Confirmation of two common mud crab species (genus Scylla) in the mangrove ecosystem of the M e k o n g Delta, Vietnam. Journal of ShellSsh Research 21:25^265.
KHOA HOC CbNG NGHl
13. Roehrdanz R. L , 1993. An unproved primer for PCR amplication of mitochondrial DNA in a variety of insect species. Insect molecular Biology 2:
89-91.
14. Rozas J., Bibrado P., Sanchez - Delbario C. J., MeseguerX., Rozas R.. 2004. DNASP version 5.00.07.
University Barcelona.
15. Starobogatov Y. L.. 1972. The Fauna of the Tonking Gulf and condition of Life in it.
Exploratation of tiie fauna of the sea. Acad. USSR.
Zool. Instil. 35: 95-415.
16. Tamura K.. Peterson D.. Peterson N., Slecher G., Nei M., and Kumar S., 2011. MEGA5:
Molecular Evolutionary Genetics Analysis using Maximum Ukelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution 28 (10): 2731-2739.
17. Thach N. C , 2003 Status of marine crab aquaculture in \'ietnam: In: Mud crab aquaculture in Australia and Soutiieast Asia. Proceedings of the ACIAR crab aquaculture scoping study and
workshop 28 29 April 2003 (Geoff, A & Don, F.
eds.), pp. 45 46. Australian Centie for International Agriculture Research, Bribie Island, Brisbane, QLD, Austialia.
18. Thai B. T., Burridge C. P., Pham T. A, Austin C. M., 2004. Using mitochondrial nucleotide sequences to investigate diversity and genealogical relationships within common carp (Cyprinus carjyio L.). Anuiial Genetics 36: 23-28.
19. Thai B. T., Pham A. T.. Austin C. M.. 2006.
Genetic diversity of common carp in Vietnam using direct sequencing and SSCP analysis of the mitochondrial DNA control region. Aquaculture 258:
228-240.
20. ITiai B. T.. Si N. V.. Phan D. P., Austin M. C , 2007. Phylogenetic evaluation of subfamily classification of the Cyprinidae, focusing on Vietnamese species. Aquatic Living Resource 20:
143-153.
21. Ngo SI Van, Thii Thanh Binh, 2005. Bio cao xic dinh tdn chinh thuc bai loii c i Triu tidn vua va Ro tdng trudng tai Ninh Binh. Bg Thiiy sin.
IDENTIFY MUD CRAB SPECIES (ScyUa sp) IN VIETNAM USING MITOCHONDRIAL DNA SEQUENCING COI REGION
Thai Thanh Bmh, Nguyen Van Viet, Christi)pher Austin, Tran Thi Trang Summary
Mud crab is high-value species and become importance aquaculture species in coastal provinces in Vietnam. AnnuaUy, approximate 100 milUon of juvenile crabs are produced from over 150 hatcheries.
Although mud crab has been cultivated in Vietnam a long time but researches about taxonomy of mud crab are limited because of mainly basing on morphometric. This study present results of identifying mud crab species using mitochondrial DNA sequencing. Thirty three mud crab samples collected from 10 provinces in Vietnam, AusUaUa were sequenced in COI region. The Vietnam mud crab sequencing also were compared with DNA sequences of mud crab in China, Australia and Indonesia. Results showed COI region is useful for mud crab identification. Four mud crab species was found in Vietnam: Scylla paramamosian, S.
serrata, S.olivacea and 5. tranquebaria in which 5. paramamosia is the most popular Phylogenetic supports the 5. paramamosian closer relationship 5. serrata and 5. tranquebaria than S.oUvacea species.
TTiese results have made the remarkable contribution to developing taxonomy systems as well as selecting materials for selective breeding programs and conservation of these species in Vietnam.
Keywords: Mudcrab, S. olivacea, S.paramamosian, S. serrataandS. tranquebaria, taxonomy Ngudi phan bi^n: TS. Nguyen Thi Thanh Thuy
