o c C O N G N G H E S I N H H O C TOAN QUOC 2013
HOAT TiNH K H A N G K H U A N . C H 6 N G OXY HOA VA (fC CHE SINH T R U I N G THyC VAT CCiA DICH CHltT VCTI METHANOL TLf CAC BO PHAN K H A C NHAU C O A C A Y C R O {ACANTHUS ILICIFOUUS L.)
Bko Van TSn'", O^o Thj Sen\ Nguyen Thj Thu Huyen\ Hokng Van Chinh"
'Khoa Sinh Hgc, Tnrdng Dai hgc Saph^m Hi Ndi 'B^I Hgc Hdng DOc, Thanh Hoa
TOM T A T
Ciy d ro (hp Acanthaceae) la loai tfayc vat n g ^ man mpc pb6 biSa 6 viing nuoc lg Vift Nam, dugc nguoi dan dia phuong sii dyng nhu mgt cay thuoc nam de chdt^ viem. Trong b ^ cao nay, chiing toi dai hanh khao sat m$t s6 boat tioh sinh hgc cua c ^ mau re, than, li, hoa va qui ciy d ro lay tii viing rimg ng|p mSn Vuon Quoc Gia Xuan Thiiy, Nam Djnh. Djdi chiit voi dung moi medianol tii cac bS ph3n ciia cdy dirge tien h^nh blngSoxhlct Kit qua thii tinh khang khuan tren 5 chung vi kbuln (E.coli, V.
paraiiaemofyticus, V. ovan,B. subtilis va S. aureus) cho thay dich chill tir hoa va qua co tinh khang voi 3 chiing vi sinh v3t thii nghidm, trong do djch chiet tir Ik co kha nSng khing v6i 2 chiing vi sinh vft thii nghiSm trong khi dicb chiSt tir than va r§ cbi khang voi 1 cbiing vi sinh vat thu o g h i ^ Boat tinh chong oxy hda dugc danh gia thong qua kha nang qu6t goc tu do DPPH ciia cac djch CMIL B § ph?n CO ho3t tinh qii6t g6c tv do DPPH manh nhit 14 i l gji tri ICjo la 52,5 pg/mt), bg phan co hofil tinh nay t h ^ nhit li hoa (gia tri ICso I& ^37,5 fig/ml). Cic dich chigt vdi methanol ciia cac b$ pt^n & ro d6u co kha nang uc chl sinh trudng ri mam hat cai cti, til 65-72% % tung bp p h ^ .
Tv khoa: Acandms ilicifolius; khing khuan, chdng oxy boa, iic chl stub tniong thvrc v|t, djch chilt ven methanol.
MdDAU
Theo Xb chirc FAO (2004) kho3ng 80% dSn so cr cac nu'oc dang phat bien si> dyng nguSn du^c liSu buy^n th6ng d^
chOa trj b^nh ban dau. Nhi>ng thuoc chQa trt benh cho ngiKri cd ngu6n goc h> cke san pham tif ntiien dif^c sv dgng 0\hu trj gan 90% so b§nh Oirgc bill den (Newman va Cragg, 2007). Wl^t Nam Ik mOt nu«c c6 di§n tfch rimg ng#p m$n Id'n, trSn 400 nghin ha vd nhiJng hieu biit vk nhung ddc tinh Oagc li$u o k cay ng#p m3n gin day da du^c biet den. Vi v$y, nghien cuu cac d^c tinh sinh hpc se co vai trt Idn d6i vdi ngu*i d§n dja phu-ang cung nha tiem nang khai thac san phim duoc ligu mi>i cCia cay ng^p m#n, m6t nguon cung cap kha Idn d Vi^t Nam.
cay 0 rd (Acanthus Ilicifolius) la lodi cky ng^p m^n ph6 biln bong vCing ni/6c lo cua Vi#t Nam(H6ng et al, 1999). Day id toki cSy Oagc nhkn Okn mpt s6 nude frSn t h i gioi nhif An O0(Singh et al. 2009) va Vi^t Nam si> dung trj nhi>ng vit viSm (H6ngetal,1999vd nhu-ng nghien ci>u v l mpt so hogt tfnh sinh hpc bong cay a ro frSn t h i gi6i dang dtrac quan t§m gin Oky. Bobbarala vk dong tkc gik (2009) da thi> nghigm 50 lodi cSy ng^p m^n d l kilm ba tinh khang doi voi vi khuan Pseudomonas syringe va cung chl ra Id cay 6 rd cd hogt tinh khang duvng(Bobbara1a et al, 2009).NhDng dan ligu lign quan din hogt tinh khdng khuin, khdng nam dirgrc Oaa ra bdi Khajurevd dong tac gia (2010), Ganesh vd dong tac gid ndm (2010); hay hogt tinh ching viem loet (Kumar et al, 2012), chong ung thir (Khajure vd Rathod, 2011) cung Oagc ddnh gid gan ddy. CQng theo hu'd'ng ddnh gid hogt b'nh sinh hpc cua cdy 6 rd, chung toi tien hanh khao ^ t khd ndng khdng khuin ben nhirng dli h/ong v< sinh vgt khac vdi nhOng tac gia cong b6 tnrac day cung nhu- hoat tinh ch6ng oxy h6a. hogt tinh i>c chl sinh tradng thi/c v^t cua djch chiet th6 vcri methanol bing phu'O'ng phap chilt Soxhiet nhdm bo sung thdng tin v l tiem ndng duvc ligu cOa lodi cay ngap m|n nay.
NGUYEN Ll^U VA PHlfONG PHAP NGHIEN COU Nguydn lieu
Cdc miu tu-oi r l , than. Id, hoa qua cua 5 ro (Acanthus ilicifolius) thu thap tir Vifcrn Quic Gia Xudn Thuy, Nam Dinh; hgt cdi cu (Raphanus sailvus L.); cdc chung vi sinh vat kiem ^nh: Escherichia coli. Bacillus subiilis, Staph^ocoixus aureus.
Vibrio ovan. Vibrio parahaemolyficus dupe phong thi nghigm Cong nghg Sinh hpc - Vi sinh hpc, Khoa Sinh hpc, Tnrcmg Dgi hpc Su" phgm Hd N0i cung d p , cac chOng nay Oirgc mua tir Vign Ve sinh Dich te Tnjng (tang.
Phirang phdp nghien c i ^ Thu vk xd'If mau
Cdc miu r l , thdn, la hoa vd qua thu dvpc bao qudn lanh, dem v l phong thi nghigm rira sgch bang nirdc mdy, sau dd sly khfi o nhigt d$ OO^C trong thdi gian 72 gift.
Chiit rut mau
30 g miu khd dd nghien nho dvcrc chilt nit 10 lln vtn 750 ml methanol 98% sO dung may chiet Soxhiet 500ml ciia Qire.
Sau at. dich chiet dvpc cho bay hoi, cfl egn ttiu dvgc keo. H6a tan keo da cd can v6i nvcrc vd din the tich djch d i i l t din 20ml. D l ling, djch hoa tan thu dvpc gpi Id djch chiet thd.
Thir hopt tinh Ifhing khuan
Hogt tinh khdng khuin dvpc xac dmh theo phvcrng phdp khuich tdn ben mdi trudrng ttigch theo Murray va ding tdc gil(1995)di,ra vao dvdng kinh vung v6 khuan (D, mm).Dijdng kinh viing vd khuin dvprc xdc djnh bdng dvdng kinh vong bdn vd khuin (bao gom vOng cd thach vd khdng cd thach) tn> di vong frdn l5 thach (khdng co thach).Cac thf nghifm dviTc nhdc lgi tir 2-3 lln 100 pi nvdc cat vd b^ng dung lam d l i chimg dm va 0,1 ml chloramfenicol 0,04% lam doi chimg dvong.
Hpl NGHI KHOA HQC CONG NGHf SINH HVU
Thir hoat Unh ch6ng oxy hoa „ . . . . ^ . . - „ - ,. . Tl,Lr host Unh ch6na oxy h6a bar» phirong phiP <!"« g6c W * Z ^ - ^ H ^ > ' j , ^ 1 „ , j ^ j ^ j , . ^ ^ dSn cOa Vi«n D w o ISu - M Y r f (2006).Dung d|oh ^ ^ ' ' " " • " / ^ o ' ^ h i ? g ™ m i i i chSng oxy hoa d w do t r * mi, hole iJ6i diiJng theo H l« 19:1 * nhiet <K phOng trong thfti gran 30 Phut. l-ac ' ^ • ' ^ . '^,„^ ^ dgnj ISm d i Zm Ph4 S^dSu dec 96 giing cOa hing Biotek, MJ ''^^?°XZZJ^'tC^S«,^H 0,112 m g * n l ^ chuSn. nmirc o4t dm,c s i dgng ISm miu dS, ohLmg. <^„^J^S,iroSi^S% (Scavenging capacity) theoca,, n g h i ^ dove nhic lai hai iSn. KhS ning quSt g6c ti,> do d w c xac oinn vao g,a u i
thi>csau:
SC% = 100 0D„ - OOBI , ODct
Trong do OD.p gia til hSp thy quang ciia mSu thi nghi$m;
ODct: giS til hdp thu quang cOa mSu d6i chimg.
ODei: giS tli hip thg quang cua mSu tring
khd nSng qudt glc tg do,
Thif ho^t tfnh dc chi sinh tradng Oige v$t
Hgt cdi CO {Raphanus saiivus L.) cho ndy mim 24 gid d dilu kign nhigt dg I n djnh (30=C) d l tgo cdy mim Sau khi cho ndy m l m n L g cdy mim od r l ding diu nhau. cd kich thvde trung binh 0,85 ± 0,15 cm Oagc chpn d l ben hdnh ft.
nghi$m Chia ^ y mim thanh cdc Id thi nghigm, m8i Ifl c6 ti; 10-15 cdy mim. Dich chilt tho dvpc si/dyng dl&«
S i m khd ndng'^dc chl sinh birdng cua mim r l c^\ cu. Diu r l ^ y mim dvpc nhung ^ " 9 v^o c^c dich ch.lt thfl b « j thdi gian 1 gid. Cdy mim dli chdng thay dich chilt thd bing nirdc cit. Sau dd, dva cdy mim vdo dilu ki#n ndy mk ban diu bong 24 gid, bin hdnh do chilu ddi r l mim. Mli cdng thdc thi nghipm dvpc nhdc Igi 3 idn.
Khd ndng dc chl sinh birdng r l dvpc tinh theo cdng thdc:
l % = (Le-L,)x100/U Trong dd:
I %: phin trdm dc chl
U: DO ddi bung binh (cm) ciia cdc r l d miu dli chvng U: Dd ddi trung binh (cm) cua cdc r l d miu thi nghigm Xiy iys6 Iliu
s l ligu dvpc XVII bfln frfiln mim Excel vd SPSS.
K £ T Q U A V A TH A O LUJjM Ho^t tfnh khkng khuin
Tit cd cdc djch chilt cua cdc bi} phdn diu c6 hogt tinh khdng khuan Cedng 1). Djch chilt cua hoa vd qua t h i hign linfi khdng mgnh hon so vdi djch chilt tir thdn, r l , Id. Djch chiet cOa hoa vd qua cd tinh khang dvong dli vdi vi khuin E. cof trong khi djch chilt Id, thdn, r l thi khflng phdt hign thiy tinh khdng vdi chiing vi sinh ndy. T i t cd cdc djch chilt cOa ck bO phgn diu cd hogt tinh khdng vi khuin V. Parahaemolyticus. Hoat tinh khang cao nhit d djch chilt ciia hoa vdi OudK kinh viing vfl khuin quan sat dvpc la 20 mm va thip nhat d thdn, vdi dvdng kinh viing vfl khuan la 8,8 mm. Djch chia methanol cOa qud, hoa vd la thi hign hogt tinh khdng ddi vdi vl khuin B. subtilis.
Bing 1. Ho^l tinh khiiig khuin (D, mm) ciia djch chiet methanol ciia cac bp ph|n ciia 6 ro B9 ph^n
R l Than
Ld Hoa O u i
£ CO*/
10 ± 2 , 4 - 13,312,3*"
Chiing vi khuin V. parahaemolyticus
13,3 ± 2 , 3 ' 8,7 ± 2,3"
7.3 ±1,2**
20,0 ± 2,0**' 15,3 ± 2 . 3 * '
V, ovan S. aureus B subtilis
2.0 ± 0,0**' 11,0 ±1.4*" I 1 7 , 0 ± 1 , 4 " ° "^
(SiH^uthi hi^n dudi dang gia tr) Irung binh ± rfO l^ch chuin. S6 ll$u dtrqc so sknh Iheo kiim dinh student, bing phumg phip LD& ^ mdc y nghTa li 0,05. Cic kl hi$u * vi " bieu thj so sanh cic gii trj theo hing ngang, cac ki hieu a, b, c biiu thi so sinh gilra cic so W (rong ciing cpt.)
Khajure vd ding tac gia {2010)vd cflng sa Ok nghidn ciru v l kha nang khdng vi sinh vgt cua cdc djch chilt khdc nha cua Id vd r l cdy d rd trong dd cd dich chilt methanol (Bobbarala et al, 2009). K i t qua nghidn ci>u cua cdc tdc gia W cho thiy d|ch chiet methanol cOa la khdng S. subtilis va S. aureus vdi dvdng kinh vilng vfl khuin Id 17 mm vd 12 IT:"
PC C 6 N G N G H ^ S I N H H O C TOAN QUOC 2013
vd d|ch chilt cua r l cdy fl rfl ^ vdi tiai vi khuin nay l l n hrpt la Id 15 nvn vd 14 mm. Trong nghien ciru ndy cita chi/ng tdi, djch chilt cua Id c6 ho^t tiitfi khdng y ^ vdi vi khuin B. subtilis, khdng bilu hign tinh khdng d l i vdi vi khuin S.
aureus. Tuy ntiien,djch chilt methanol cua r i cay d rd khdng the hien hoat tinh khang khuin vdi tdt cd cdc chung vi khuin thd nghigm tiir V. parat}aemolyticus.So sanh vdi cac lodi thgc vgt nggp man ididc (Singh et al, 2009, Bobtiarala et al. 2009, Khajure el al, 210. Kumar et al, 2012. Ganesh vd Jannet 2010), khd ndng khdng khuin t^a cdy fl rfl Ididng
Ho$t tinh ching oxy fi6a
oh nghidn ciru hogt b'nh chong oxy hda cua djch chiet methanol b> cac phin khdc nhau cua cdy 6 rd, chOng tfli khdo sat ben day ning dd dich chiet vdi cdc mirc do pha lodng: 4,10. 20, SO. 100 lln. Gia fai ICsodvcrc b'nh todn dva vdo hdm hrang quan nong dfl chit tan va kha nang quet g l c tv do DPPH.Ket qud thu dvcrc the hign trong bang 2.
Bang 2. Ho^t tinh ch6ng oxy hoa cua (Qch chiet tho mtihanol ti> cac b$ ph|n ciia cay 6 rd B«pl4n
R l Thdn Ld Hoa Qud
I C M ( ( I 9 ' R > Q 52.5 207 445.5 537,5 219.5
% s o v d i acid ascorbic 3.99
1.01 0.40 0.33 0.82
hk>at tfnh ching oxy hda mgnh nhit I d d r l c d y d r d v d i ^ t i ICso Id 52.5 pg/ml tvcmg dvong 3,99% hoat tinh chong oxy hda ciia add ascorbk;, sau dd d i n thdn 6 rfl vdi gia tri ICso la 207pg/rnl (tirong dvcmg 0,82% hogt tfnh cda add ascortNc), yeu nhit Id d hoa vdi gid bj ICso Id 537,5pg/ml (tvong dvong 0.33% hogt tinh cua add asovbic). So sdnh vdi hogt tinh chong oxy tioa ciia add ascortMC, cd t h i ttily ring djdi chilt methanol cua cdc bfl phan cOa cdy 6 rfi cd hogt tfrdi ching oxy hda y l u .
Banefjeevd vd ding tac gid(2008) da ben hdnh nghien ciru v l kha nang ching oxy hda dia 10 loai cdy ngdp mgn trong dd cd djch chilt methanol 20% tir la vd r l ciia cdy 6 rd. Cdc gia tri 'Cso ciia djch chilt b> Id vd r l dvpc cdc tdc gid ndy ch?
ra tvong dng la 2501,53 pg/ml va 1319,66 pg/ml. Nhv vgy, hogt tinh quet glc hr do cOa r l bong nghien cdu cOa chung tdi m^nh horn so vdi nghi&i cihi tnrdc ddy. Asha va ding tac ^ ( 2 0 1 2 ) Igi dan ra s l ligu v l b'nh chong oxy hda cOa cdy d r d v a ^ c h l r a g i a b l ICso cua dtch chilt methanol cOa cdy fl rfl Id 27,6 pg/ml. Tuy nhifln, tdc gid ndy khflng phan tdch rfl ho^t tinh qudt g l c tg do DPPH theo Kmg bp p h ^ . Nhv vdy, cd si/ sai khdc v l hogt tinh chong oxy hda ciia cdy d rd giva cdc tdc gia vdi nliau. Sg sai khdc ndy cd the glai thich bdi sg khdc nhau v l cdc dilu kign thf nghigm cung cd t h i do ngudn vdt li$u liy tir cdc vdng khac ntiau.
Ho9t tinh ire chi sinh tnrdng ri mim cai cQ
Sau khi XVII F I vdi dK^ chilt bid 1 gid, dva re mim v l dilu kien ban diu 24 gid, tien hanh do chieu dai r l cua cdc Id thi nghigm. So sanh gid bi trung binh chilu dai r l gida Id tht nghiem vd d l i chirng cd sai khac cd •j/ nghTa thi djdi chilt thd dv^c coi la cd hogt tinh dc d i l sinh budng. Kit qud so sanh chieu ddi tmng binh cua chilu dai re mam ciia mau dli chdng va m i u thi nghigm sau khi xir li bang cdc djch chilt tir cdc t>d phdn khac nhau cd sg> sai khdc cd ^ nghTa ( P £ 0.001). Nhv vdy, t i t ca cdc dich chiet vdi dung mdi methanol deu gdy ire c h l sinh bvdng r l mim cai cii. Kha nang ire chl sinh bvdng r l mim dirpc danh gid qua gia hi I (%).
Bdng 3. Kha nang d c c h l sirdi t n r d n g cOa (Qch chiet c i c t>fi phan cua cdy 6 r d [I, %) B d p h d n
Rl Thdn
Ld Hoa Oud
G i d t r i l ( % ) 67.1 ± 3,8 67,0 ± 3,4 72.6 ± 3,6 68.1 15.4 65.0 ± 5.9 (S6 S$u thi hi$n dudi dang ga trj tnmg binh ± dp 1^ chuin)
Djdi chilt cdc t>p phgn ciia cay d rd vdi dung mdi mettianol diu dvpc quan sat cd hogt tinh vc c h l sinh trvdng cOa Uivc v$L Kit q u i so sdnh cdc gid tri trung binh khdng ttily sir khdc nhau cd y nghTa (P>0.05) ttieo tieu chuan Student gida cdc djch chilt tir cdc bp phan khdc nhau v l kha nang vc c h l sinh bvdng thi/c vaL So vdi kha nang dc c h l sinh tnrdng thi/c vgt cua cdy cdc ken (Derris trifc^iata) [14]. kha ndng ire c h l ^nh trvdng thvc vdt ciia djch chiet cua cay d rd deu mgnh hon.
K ^ L U A N
Cdc bfl phgn cOa cdy d rfl dvpc chilt vdi dung mfli methand diu the hign hogt tinh khdng khuan. hogt tinh chong oxy hda vd kha nang vc die smh tnrdng r l mim cdi cii. Djch chilt td hoa va qua the hign b'nh khdng vdi nhilu vi sinh vdt thd nghigm nhat vd muc dfl khang vdi cdc vi sinh vgt ndy cung cao hon so vdi cdc bp phdn cdn lai. ChOng vi sinh vgt V.
parahaemolyticus nhay cam nhit vdi b'nh khdng cua cdc dich chilt t v cac bfl phgn ciia cay fl rfl.
HOI NGHI KHOA HOC CONG NGH? SINH HOC
. , . . , . , . , J , , , , ri„ D P P H ciia dich c h i i t l i r cac b O p h S n c S y 6 l f i i i h a n g c a o . R l S o v o i chSt chuSn a a d d ascortjic, hoat t i n h q u « ^ " f "J^iu IC«, li 5 2 5 u g / m l , t h a p h o n 4 d e n 1 0 iSn g i a t n n i y
IS bO p h « n t h i hi«n k h a nang q u e t g 6 c W d o manh nhSt vfti gia tn 11,50 B O A = pa/ ^ c u a c d c t)d p h a n k t i a c . , . . , . ^ .
. , . „ „ eat ihAv CO kha n a n g l i e c h l s i n h t r u d n g l i m a m c a i c i i v^
T i t cS cac dich c h i i t t i r cac b o p h a n cay a ro d i u dinjrc q u a n sat thay co kna n a n s Sir k h a c biat gitra v i kha n a n g nay cua cac bO phan khang ro.
T A I u e u T H A M K H A O
J h™,nii,. mmnniinds in two mangrove species and ttieir antioxKlanI Asha K.K. Malhew.S and L a k s l m v r a i F T . (2012). Flavonoids and phenolic compounds i « o m.na i~v properly./rKfen J Geo^*anneSci'.41 (3): 25^.264. . , ,. . B , ™ , e e D ChaKraban, S. H a ^ ^K Baned-e S, Ray J and K . „ K h = , . . B, (2008). A n , l . . d a n t . c , » » y and total phenolics o, s o , , m a i o o v e s in Sundarbans. Afr J Bk>lechnol.7(6): 805-810.
B o l * , r , , a V , S „ n , . a s a R a o G , A r , . r n i , h , , D , N a i d u K C . R a o G . S ( 2 0 0 9 ) B a c , . n = i . l a c t i v i t y o , » y m « , i c , n a l p l a n t s m . a , a n „ , i c , « « against Pseudomonas syringae pv- Syringae. BPJ. 2(1) 61-66,
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Ganesh S and Jannet J (2010). Vennlla screening for Antmk^robial Activity in ^ c a n f h u . i t o f o / , . a ^
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Khajure P.V and Rathod J.L. (2011). Potential anticancer activity of Acanthus ilicrhllus axtracted from the mangroves forest of Kan™.
west coast of India. WJST. 1(1):01-06.
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Mun^y P,R. Baron E. J . Pfaller M A Tenover F.C and Yolken H.R (1995). Manual of clinical microbiohgy 6^. The American Societyft Microbiology Press . 1482 pages
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SinghA, Duggal S, Suttee A (2009),>*can(/7us ilicifolius linn -lesser known medicinal plants witii significant phamiacological activities.
Na[Prod,1-1-3.
Ddo v a n T i n Ddo Thj Sen VQ Thi Hien (2012). Khdo sdt mot s6 ho^t tfnh sinh hpc cua dich c h i i t t d hat cdy coc ken (Derris ftrfoW Lour) d Virdii Q u i c gia Xudn TTiiiy, Nam Ojnh. Bao cio khoa hoc ve nghlSn cOu vi day hoc sinh hpc d Vi$l Nam. Nhd XuSl bin Nfln nghiOp. B70-676-
Vi^n Duvc lieu - B$ Y Te (2006). Phtrong phip nghiin cdu tic dung dugc ly cda thudc tir DiriTC thio, NXB Khoa hpc Ky thugt HS m tr. 279-292.
ANTIBACTERIAL AND ANTIOXIDATIVE ACTIVITIES AND PLANT-GROWTH INHIBITION BY METHANOLIC EXTRACTS FROM VARIOUS PARTS OF THE MANGROVE PLANT >AC>!\A/THUS ILICIFOLIUS L.
Dao V a n T a n ' , Dao T h i S e n ' , N g u y e n T h i T h u H u y e n ' , H o a n g V a n C h i n h ^
^Faculty of Oology, Hanoi National University of Education i
'Hong Due University, Thanh Hoa - I
SUMMARY
Acaniiius ilicifolius (Acanthaceae), a common mangrove plant in brackish water areas, is widely used as an anti-inflammi.
medicmal herb by the local peoplcln this study, methanolic extracts from vanous paitsof Acanilius tbcifolous collected fromlt Thuy National Pait, Nam Dinh Province, including roots, stems, leaves, flowers and fruits, were evaluated for their antibaclensli antioxidative activities and inhibition of plant growth. Five bacterium strains, including E.coli, V. parahaemolyticus, V. ow Subtilisand S. aureus, were used for testing antibacterial activities ofthe extracts. The exttacts from flowers and fhiits were obiB effective against the 3 bactenal strains E cob, B, subiilis and V. parahaemolyticus, the exhact fiwn leaves showed antiba*
activities against the 2 sU^nsK parahaemolyticus and B. subtilis. Antibacterial activities of stems and roots were observed S baclenal suainf^ parahaemolyticus DPPH free radical scavenging activities on crude methanol extracts were evaluated' maximum radical scavenging activity was observed for Ihe extract from roots (IC50 value, 52.5 jig/ml) and the minimum Bi scavanging acuvity was shown by the extract from flower (IC50 value, 537.5 (ig/ml). All parts ofthe plants showed inhibition ii growth of seedling roots, ranging from 65% to72%, depending on parts of Ihe plants.
Keywords: Acanthus ilicifolius. antibaterial activity, antioxidative activity, plant growth inhibition, methanolic extract.
Author for conBsspondence Mobe: +84-936696931; Email, [email protected]
