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Lampiran 1 Luas infeksi virus tungro pada tanaman padi tahun 2007-2011
di beberapa kabupaten di Jawa
Kabupaten 2007 2008 2009 2010 2011 T P T P T P T P T P Bogor 256 1 156 - 90 - 151 - 105 - Pandeglang 378 28 516 - 256 18 630 - 229 1 Subang 315 20 220 - 169 - 81 - 186 - Batang 340 5 255 - 253 - 300 - 251 - Klaten 65 - 96 - 54 - 65 - 41 - Sleman 36 - 110 - 44 - 77 - 127 - Jember - - 145 10 145 10 371 3 41 8 Banyuwangi - - 159 1 159 1 245 2 173 - Keterangan: T: Terinfeksi (satuan dalam hektar)
P: Puso (satuan dalam hektar)
- : Menyatakan tidak ada infeksi atau luas infeksi kurang dari 1 hektar Sumber: Direktorat Perlindungan Tanaman Pangan, Direktorat Jenderal Tanaman
Lampiran 2 Luas serangan wereng batang coklat pada tanaman padi tahun 2007-2011 di beberapa kabupaten di Jawa
Kabupaten 2007 2008 2009 2010 2011 T P T P T P T P T P Bogor 12 - 4 - - - 96 - 4 - Pandeglang 2 444 - 624 - 345 - 6 092 330 2 895 14 Subang 4 168 - 2 057 - 1 681 - 22 688 1 167 344 - Batang 75 2 113 1 543 1 194 - 1 584 42 Klaten 809 - 28 - 803 - 4 831 584 4 237 2 Sleman 19 - 1 - 71 - 566 - 6 503 1 976 Jember - - - - 1 101 85 3 770 199 2 154 7 Banyuwangi - - - - 516 48 644 29 1 588 64 Keterangan: T: Terinfeksi (satuan dalam hektar)
P: Puso (satuan dalam hektar)
- : Menyatakan tidak ada infeksi atau luas infeksi kurang dari 1 hektar Sumber: Direktorat Perlindungan Tanaman Pangan, Direktorat Jenderal Tanaman
Lampiran 3 Hasil amplifikasi gen CP RTBV dan RGSV dari sampel tanaman padi dari beberapa kabupaten di Jawa.
Kabupaten Dusun/Desa/Kecamatan Hasil Amplifikasi RTBV RGSV Subang Cijambe + - Sukasari - - Tanjung Siang + - Patok Besi - + Pabuaran - + Pamanukan - - Ciasem - + Blanakan - - Pandeglang Sukamanah - + Cening - + Muruy - - Munjul - + Panarayan - + Sampang Bitung - + Kuronji - + Cimanuk - +
Bogor Situ Gede + -
Cikarawang + + Muara + - Ciherang + + Sukamaju Cibungbulang - - Pamijahan + - Ciampea - + Gunung Bunder + - Klaten Plawian - - Granting - - Prawatan - - Kraguman - - Joton - - Ngemplak - - Tambak Watu - - Joton 1 - +
Keterangan: +: Hasil amplifikasi yang menunjukkan sampel tanaman positif terinfeksi RTBV atau RGSV
-: Hasil amplifikasi yang menunjukkan sampel tanaman negatif terinfeksi RTBV atau RGSV
Lampiran 3 Lanjutan
Keterangan: +: Hasil amplifikasi yang menunjukkan sampel tanaman positif terinfeksi RTBV atau RGSV
-: Hasil amplifikasi yang menunjukkan sampel tanaman negatif terinfeksi RTBV atau RGSV
Kabupaten Dusun/Desa/Kecamatan Hasil PCR
RTBV RGSV
Batang Sidomulyo - +
Luwung - -
Kali Salak Timur - +
Boja - +
Kali Batik - -
Harjowi Barat - +
Sembung + -
Kali Salak selatan - +
Sleman Jetak 2 Barat - +
Mandungan - + Prenggan - + Ngawen - + Semarangan - + Jetak 2 Utara - + Jetis Timur - + Jetis Barat - +
Jember Sumber Kejayan - +
Sumber Bulus Barat - +
Sumber Salak Tengah - -
Sumber Salak Timur + -
Sumber Salak Barat + -
Garahan Barat - -
Sumber Bulus Utara - +
Garahan Timur + - Banyuwangi Songgon - + Balak - + Parang Harjo - - Gumirih - - Sumber Kencono + - Singo Juruh - - Sragi - + Benelan Lor - -
Lampiran 4 Varietas, umur, dan gejala umum sampel tanaman padi dari beberapa kabupaten di Jawa.
Sampel Tanaman
Varietas Padi Fase/Umur Tanaman*
Gejala Umum Kabupaten Desa/Kecamatan Warna
Daun
Tinggi Tanaman
Jumlah Anakan Subang Cijambe IR 64 14 HST KO Kerdil Sedikit Sukasari Ciherang Primordia KO Normal Sedikit Tanjung Siang IR 64 14 HST KO Kerdil Sedikit Patok Besi Ciherang Singgang KO Normal Normal Pabuaran Ciherang 14 HST KO Normal Sedikit Pamanukan Ketan Bunting KO Normal Sedikit Ciasem Ciherang 14 HST KO Normal Normal Blanakan Ciherang Primordia KO Normal Sedikit Pandeglang Sukamanah Ciherang Singgang KO Normal Normal Cening Ciherang 56 HST KO Normal Sedikit Muruy Ciherang Primordia KO Kerdil Sedikit Munjul Ciherang 28 HST KO Normal Sedikit Panarayan Ciherang Primordia Kuning Normal Sedikit Sampang Bitung Ciherang 56 HST KO Normal Normal Kuronji Ciherang 56 HST KO Kerdil Sedikit Cimanuk Ciherang 56 HST KO Normal Sedikit Bogor Situ Gede Ciherang 42 HST KO Kerdil Sedikit Cikarawang Ciherang 42 HST KO Kerdil Sedikit Muara IR 64 28 HST Kuning Kerdil Sedikit Ciherang Ciherang Singgang KO Kerdil Sedikit Sukamaju IR 64 Primordia KO Kerdil Sedikit Pamijahan IR 64 42 HST KO Kerdil Sedikit Ciampea TDK 56 HST KO Kerdil Sedikit Gunung Bunder IR 64 42 HST Kuning Kerdil Sedikit Klaten Plawian Situ Bagendit 20 HST KO Kerdil Sedikit Granting Inpari 13 50 HST Kuning Kerdil Sedikit Prawatan Situ Bagendit 45 HST KO Kerdil Sedikit Kraguman Ciherang 20 HST KO Kerdil Sedikit Joton Ciherang 25 HST Kuning Kerdil Sedikit Ngemplak Situ Bagendit 45 HST KO Kerdil Sedikit Tambak Watu IR 64 21 HST Kuning Kerdil Sedikit Joton 1 Ciherang 50 HST KO Kerdil Sedikit *Keterangan: HST : Hari Setelah Tanam
Primordia: Pembungaan
Singgang : Tanaman yang tumbuh dari tanaman setelah dipanen KO : Kuning sampai oranye TDK: Tidak diketahui
Lampiran 4 Lanjutan Sampel Tanaman Varietas Padi Fase/Umur Tanaman * Gejala Umum Kabupaten Desa/Kecamatan Warna Daun Tanaman Tinggi Anakan Jumlah Batang Sidomulyo IR 64 35 HST KO Normal Sedikit Luwung IR 64 14 HST KO Normal Sedikit Kali Salak Timur IR 64 21 HST KO Normal Normal Boja IR 64 Primordia KO Kerdil Sedikit Kali Batik IR 64 70 HST KO Normal Sedikit Harjowi Barat IR 64 21 HST KO Kerdil Sedikit Sembung IR 64 21 HST KO Kerdil Sedikit Kali Salak Selatan IR 64 21 HST KO Kerdil Sedikit Sleman Jetak 2 Situ Bagendit 50 HST KO Kerdil Sedikit Mandungan Inpari 1 80 HST KO Kerdil Sedikit Prenggan Situ Bagendit 50 HST KO Normal Sedikit Ngawen Inpari 8 35 HST KO Normal Sedikit Semarangan Situ Bagendit 30 HST KO Normal Sedikit Jetak 2 Utara Situ Bagendit 70 HST KO Kerdil Normal Jetis Timur Situ Bagendit 35 HST KO Normal Sedikit Jetis Barat Situ Bagendit 30 HST KO Normal Sedikit Jember Sumber Kejayan Ciherang 45 HST KO Kerdil Sedikit Sumber Bulus Barat Ciherang 45 HST KO Kerdil Sedikit Sumber Salak Tengah Ciherang 45 HST KO Kerdil Sedikit Sumber Salak Timur Ciherang 40 HST KO Kerdil Sedikit Sumber Salak Barat Ciherang 45 HST KO Kerdil Sedikit Garahan Barat Ciherang 35 HST KO Kerdil Sedikit Sumber Bulus Utara Ciherang 40 HST KO Kerdil Sedikit Garahan Timur Ciherang 60 HST KO Kerdil Sedikit Banyuwangi Songgon Bondoyudo 30 HST KO Kerdil Sedikit Balak Bondoyudo 50 HST KO Kerdil Sedikit Parang Harjo Bondoyudo 30 HST KO Kerdil Sedikit Gumirih Inpari 13 40 HST KO Kerdil Sedikit Sumber Kencono Inpari 13 45 HST KO Kerdil Normal Singo Juruh Inpari 10 30 HST KO Normal Sedikit Sragi Inpari 13 75 HST KO Kerdil Sedikit Benelan Lor Ciherang 50 HST KO Kerdil Normal *Keterangan: HST : Hari Setelah Tanam
Primordia: Pembungaan
Singgang : Tanaman yang tumbuh dari tanaman setelah dipanen KO : Kuning sampai oranye
Lampiran 5 Runutan basa nukleotida gen protein selubung isolat-isolat RTBV dari beberapa kabupaten di Jawa dan Asia (GenBank). Tanda * (bintang) menunjukkan basa nukleotida yang identik
Isolat
2420
Cijambe T-CAGAAATT AGGCCAACC- AAACGCCCTA AAGAAGAACA AACAGCCTAT Cikarawang T-CAGAAATT AGGCCAACT- AAGCGTCCTA AAGAGGAACA AACAGCCTAT G.Bund Tmr --- --- --- --- --- Sembung T-CAGAAATT AGGCCAACA- AAACGACCTA AAGAAGAACA AACAGCCTAT S. Kencono A--AAAAGTT T--TCAATAT CTTCGTCATC TGATGCTATA GATA--CTAT Tj. Siang TTCAGAAATT TGGCCCACCC AAACGACCTA AAGAAGAACA AACAGCCTAT