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LAMPIRAN
Lampiran 1 Data Gonado Somatik Indeks(GSI) belut sawah setiap minggu selama penelitian
Perlakuan
Minggu NaCl PMSG hCG AD PMSG+hCG PMSG+AD hCG+AD
M0 0.1 0.1 0.1 0.1 0.1 0.1 0.1 M1 0.15 0.16 0.33 0.15 0.13 0.17 0.54 M2 0.12 1.56 0.96 0.08 0.87 0.67 1.59 M3 0.17 0.38 0.59 0.23 1.4 3.98 0.68 M4 0.1 0.25 0.43 0.09 0.74 4.36 1.25 Total 0.642 2.453 2.408 0.648 3.245 9.282 4.156 Rata-rata 0.128 0.491 0.482 0.130 0.649 1.856 0.831 Stdev 0.032 0.607 0.321 0.062 0.545 2.128 0.591
Lampiran 2 Data dan analisis Gonado Somatik Indeks (GSI) belut sawah pada minggu ke-4
Perlakuan
Ulangan NaCl PMSG hCG AD PMSG+hCG PMSG+AD hCG+AD 1 0.135 0.123 0.431 0.142 1.020 4.358 1.038 2 0.096 0.249 0.262 0.128 0.278 3.498 1.248 3 0.329 0.659 0.147 0.202 0.743 2.215 0.531 4 0.157 1.696 0.927 0.274 1.652 2.925 0.713 Total 0.718 2.727 1.767 0.745 3.693 12.995 3.531 Rata-rata 0.180 0.682 0.442 0.186 0.923 3.249 0.883 Stdev 0.103 0.714 0.344 0.067 0.574 0.907 0.321 Software: MINITAP 16
Welcome to Minitab, press F1 for help.
One-way ANOVA: C1 versus C2
Source DF SS MS F P C2 6 27.181 4.530 16.70 0.000 Error 21 5.696 0.271
Total 27 32.877
S = 0.5208 R-Sq = 82.68% R-Sq(adj) = 77.73%
Individual 95% CIs For Mean Based on Pooled StDev
Level N Mean StDev ---+---+---+---+--- AD 4 0.1861 0.0666 (----*---) hCG 4 0.4418 0.3438 (----*---) hCG+AD 4 0.8828 0.3215 (---*----) NaCl 4 0.1796 0.1031 (---*----) PMSG 4 0.6818 0.7141 (----*---) PMSG+AD 4 3.2488 0.9067 (---*----) PMSG+hCG 4 0.9232 0.5743 (----*---) ---+---+---+---+--- 0.0 1.2 2.4 3.6 Pooled StDev = 0.5208
Grouping Information Using Tukey Method
C2 N Mean Grouping PMSG+AD 4 3.2488 A PMSG+hCG 4 0.9232 B hCG+AD 4 0.8828 B PMSG 4 0.6818 B hCG 4 0.4418 B AD 4 0.1861 B NaCl 4 0.1796 B
Lampiran 3 Data Hepatosomatik Indeks belut sawah setiap minggu selama penelitian
Perlakuan
Minggu NaCl PMSG hCG AD PMSG+hCG PMSG+AD hCG+AD
M0 1.73 1.73 1.73 1.73 1.73 1.73 1.73 M1 2.13 0.98 1.64 2.37 1.20 2.07 1.43 M2 1.08 2.92 5.78 0.71 3.17 2.87 2.61 M3 1.38 1.13 0.98 1.36 2.48 4.98 0.85 M4 1.63 2.24 3.02 0.64 1.07 3.52 1.53 Total 7.950 8.991 13.145 6.801 9.648 15.164 8.142 Rata-rata 1.590 1.798 2.629 1.360 1.930 3.033 1.628 Stdev 0.391 0.803 1.910 0.724 0.889 1.292 0.639
Lampiran 4 Data dan analisis nilai Hepatosomatik Indeks (HSI) belut sawah pada minggu ke-4
Perlakuan
Ulangan NaCl PMSG hCG AD PMSG+hCG PMSG+AD hCG+AD
1 1.76 1.72 3.02 0.85 1.28 3.52 1.14 2 1.63 2.24 3.28 0.89 2.36 3.19 1.53 3 1.81 2.31 1.62 0.60 1.07 2.56 2.39 4 1.57 2.75 2.67 3.38 1.27 2.92 2.50 Total 6.78 9.01 10.58 5.72 5.98 12.19 7.56 Rata-rata 1.69 2.25 2.64 1.43 1.49 3.05 1.89 Stdev 0.11 0.42 0.73 1.30 0.59 0.41 0.66 Software: MINITAP 16
One-way ANOVA: C1 versus C2
Source DF SS MS F P C2 6 8.921 1.487 3.09 0.025 Error 21 10.099 0.481 Total 27 19.020 S = 0.6935 R-Sq = 46.90% R-Sq(adj) = 31.73%
Individual 95% CIs For Mean Based on Pooled StDev
Level N Mean StDev -+---+---+---+--- AD 4 1.4309 1.3029 (---*---) hCG 4 2.6446 0.7311 (---*---) hCG+AD 4 1.8888 0.6610 (---*---) NaCl 4 1.6944 0.1100 (---*---) PMSG 4 2.2532 0.4206 (---*---) PMSG+AD 4 3.0466 0.4073 (---*---) PMSG+hCG 4 1.4950 0.5851 (---*---) -+---+---+---+--- 0.80 1.60 2.40 3.20 Pooled StDev = 0.6935
Grouping Information Using Tukey Method C2 N Mean Grouping PMSG+AD 4 3.0466 A hCG 4 2.6446 A B PMSG 4 2.2532 A B hCG+AD 4 1.8888 A B NaCl 4 1.6944 A B PMSG+hCG 4 1.4950 A B AD 4 1.4309 B
Means that do not share a letter are significantly different.
Lampiran 5 Data konsentrasi estradiol-17β belut sawah setiap minggu selama penelitian
Perlakuan
Minggu NaCl PMSG hCG AD PMSG+hCG PMSG+AD hCG+AD M0 22.53 22.53 22.53 22.53 22.53 22.53 22.53 M1 31.49 33.83 51.63 20.58 43.05 235.02 22.52 M2 52.66 39.77 37.58 39.51 31.43 36.3 13.63
M3 26.39 22.5 69.52 48.9 27.27 55.25 31.62
M4 58.8 16.52 33.9 29.52 42.15 42.53 95.49
Lampiran 6. Data diameter telur belut sawah setiap minggu selama penelitian
Perlakuan
Minggu NaCl PMSG hCG AD PMSG+hCG PMSG+AD hCG+AD
M0 0 0 0 0 0 0 0
M1 0 0.45 0.51 0.0 0.55 0.48 0.66
M2 0 0.71 0.80 0.0 0.64 0.86 0.45
M3 0 0.69 0.00 0.0 2.69 1.12 0.74
M4 0 0.55 0.87 0.24 2.24 3.07 0.89
Lampiran 7 Data pertambahan bobot dan panjang setiap perlakuan selama penelitian
Data pertambahan bobot tubuh belut sawah setiap minggu selama penelitian Minggu
ke-
Perlakuan
NaCl PMSG hCG AD PMSG+hCG PMSG+AD hCG+AD
M1 1.99 0.44 0.92 1.66 0.32 2.68 1.43 M2 0.22 0.24 2.23 0.64 3.24 2.42 1.41 M3 0.67 0.3 0.01 0.16 2.6 0.87 2.33 M4 1.95 0.38 0.83 1.2 1.17 1.38 0.81 Total 4.83 1.36 3.99 3.66 7.33 7.35 5.98 Rata-rata 1.21 0.34 1.00 0.92 1.83 1.84 1.50 Stdev 0.90 0.09 0.92 0.65 1.33 0.86 0.63
Data pertambahan panjang tubuh belut sawah setiap minggu selama penelitian
Minggu ke-
Perlakuan
NaCl PMSG hCG AD PMSG+hCG PMSG+AD hCG+AD
M1 0.4 0.5 0 0 0 0 0 M2 0 0.3 0 0.9 0 0 0.5 M3 0.8 0 0.3 0.6 0 0 0 M4 0 0 0 0.3 0.2 0 0 Total 1.20 0.80 0.30 1.80 0.20 0.00 0.50 Rata-rata 0.30 0.20 0.08 0.45 0.05 0.00 0.13 Stdev 0.38 0.24 0.15 0.39 0.10 0.00 0.25
Lampiran 8 Data dan analisis pertambahan bobot tubuh belut sawah minggu ke-4
Perlakuan
NaCl PMSG hCG AD PMSG+hCG PMSG+AD hCG+AD
Rata-rata 0.66 0.48 0.77 0.60 0.99 1.89 0.79
Stdev 0.86 0.21 0.49 0.51 0.64 0.91 0.27
Analisis pertambahan bobot tubuh belut sawah pada minggu ke-4 Software: MINITAP 16
One-way ANOVA: Bobot tubuh versus Perlakuan
Source DF SS MS F P Perlakuan 6 5.346 0.891 2.40 0.063 Error 21 7.787 0.371
Total 27 13.134
S = 0.6089 R-Sq = 40.71% R-Sq(adj) = 23.77%
Individual 95% CIs For Mean Based on Pooled StDev
Level N Mean StDev --+---+---+---+--- AD 4 0.5975 0.5097 (---*---) hCG 4 0.7725 0.4890 (---*---) hCG+AD 4 0.7925 0.2704 (---*---) NaCl 4 0.6575 0.8643 (---*---) PMSG 4 0.4775 0.2066 (---*---) PMSG+AD 4 1.8875 0.9051 (---*---) PMSG+hCG 4 0.9850 0.6441 (---*---) --+---+---+---+--- 0.00 0.70 1.40 2.10 Pooled StDev = 0.6089
Grouping Information Using Tukey Method Perlakuan N Mean Grouping
PMSG+AD 4 1.8875 A PMSG+hCG 4 0.9850 A B hCG+AD 4 0.7925 A B hCG 4 0.7725 A B NaCl 4 0.6575 A B AD 4 0.5975 A B PMSG 4 0.4775 B
Lampiran 9 Data dan analisis pertambahan panjang belut sawah pada minggu ke-4
Perlakuan
NaCl PMSG hCG AD PMSG+hCG PMSG+AD hCG+AD
Rata-rata 1.20 0.58 0.48 0.58 0.35 0.27 0.43
Stdev 0.97 0.33 0.05 0.51 0.31 0.30 0.33
Software: MINITAP 16
One-way ANOVA: Panjang tubuh versus Perlakuan
Source DF SS MS F P Perlakuan 6 2.242 0.374 1.64 0.186 Error 21 4.788 0.228
Total 27 7.030
S = 0.4775 R-Sq = 31.90% R-Sq(adj) = 12.44%
Individual 95% CIs For Mean Based on Pooled StDev
Level N Mean StDev ----+---+---+---+--- AD 4 0.5750 0.5058 (---*---) hCG 4 0.4750 0.0500 (---*---) hCG+AD 4 0.4250 0.3304 (---*---) NaCl 4 1.2000 0.9661 (---*---) PMSG 4 0.5750 0.3304 (---*---) PMSG+AD 4 0.2750 0.2986 (---*---) PMSG+hCG 4 0.3500 0.3109 (---*---) ----+---+---+---+--- 0.00 0.50 1.00 1.50 Pooled StDev = 0.4775
Grouping Information Using Tukey Method
Perlakuan N Mean Grouping NaCl 4 1.2000 A PMSG 4 0.5750 A AD 4 0.5750 A hCG 4 0.4750 A hCG+AD 4 0.4250 A PMSG+hCG 4 0.3500 A PMSG+AD 4 0.2750 A
Lampiran 10 Biaya pembuatan hormon perlakuan pada penelitiaan No Perlakuan Dosis/kg Harga 10 cc (10 kg) Harga 1cc (belut panjang 22±2 cm, 60-100 ekor) 1 NaCl 0.95% 1 ml Rp. 1000 Rp. 100 2 PMSG 20 IU Rp. 101000 Rp. 10100 3 hCG 20 IU Rp. 21000 Rp. 2100 4 Antidopamin 0.01 mg Rp. 10000 Rp. 1000 5 PMSG+hCG 20 IU + 10 IU Rp. 111000 Rp. 11100 6 PMSG+AD 20 IU + 0.01 mg Rp. 101500 Rp. 10100 7 hCG+AD 20 IU + 0.01 mg Rp. 21500 Rp. 2150 Keterangan: Harga PMSG 500IU/10 ml = 250000 Harga hCG 1500IU/5 ml = 150000 Harga Antidopamin 0.01 mg = 1000 Harga NaCl = 7000
Lampiran 11 Mekanisme kerja hormon perlakuan induksi maturasi belut sawah
Mekanisme kerja hormon perlakuan induksi maturasi belut sawah pada penelitian ini seperti di bawah ini:
OTAK Hipotalamus Pituitari Perkembangan Oosit (maturation/ rematuration) GONAD Sel Teka GnRH Gonadotropin Sel Granulosa T P450 arom E Vitellogenesis Anti dopamin, AD GnRH, FSH-RH endogenous FSH FSH PMSG hCG (Sudrajat, 2010)