DAFTAR PUSTAKA

PEMBAHASAN UMUM

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Lampiran 1 Ikan koridoras (Corydoras aeneus)

Corydoras aeneus disebut juga bronze catfish merupakan ikan hias air tawar yang berasal dari Amerika Selatan, di Mato Grosso, Brasil, dan Rio Paraguay. Ikan ini termasuk golongan catfish yang berukuran relatif kecil, yaitu berukuran maksimal 8 cm, memiliki warna coklat hijau keemasan dan kemilau seperti tembaga, memiliki varietas albino dengan warna putih. Ikan koridoras ini hidup pada suhu 24-30 °C, pH 6-8, kesadahan karbonat maksimal 2 dan kesadahan total maksimal 12 atau 150-180 mg/L CaCO3, alkalinitas netral atau sedikit alkalin (50-150 mg/L), serta bersifat omnivora yakni memakan semua jenis pakan baik pakan buatan maupun pakan alami (Axelrod et al. 1988, Petrovicky 1988, Satyani 2005 dan Alderton 2008). Ikan hias Corydoras aeneus

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Lampiran 2 ANOVA pada penelitian intensifikasi budidaya ikan hias koridoras ANOVA pada penelitian budidaya ikan koridoras dengan perlakuan padat tebar dan ganti air

LPH panjang Sumber Jumlah Kuadrat db Kuadrat Tengah F p Model Terkoreksi .103a 5 .021 92.270 .000 Intersep 1.059 1 1.059 4.738E3 .000 Padat tebar .044 2 .022 99.299 .000 Ganti air .040 1 .040 180.772 .000 pdtbar*gantiair .018 2 .009 40.990 .000 Galat .003 12 .000 Total 1.165 18 Total Terkoreksi _.106 ₁₇ a. R2= ,975 (Adjusted R Squared = ,964) LPH bobot Sumber Jumlah Kuadrat db Kuadrat Tengah F p Model Terkoreksi .153a 5 .031 3.634 .031 Intersep 12.937 1 12.937 1.534_E3 .000 Padat tebar .080 2 .040 4.754 .030 Ganti air .031 1 .031 3.665 .080 pdtbar*gantiair .042 2 .021 2.499 .124 Galat .101 12 .008 Total _13.192 ₁₈ Total Terkoreksi _.254 ₁₇ a. R2 = ,602 (Adjusted R Squared = ,437)

Sumber Jumlah Kuadrat

d b

Kuadrat

Tengah F p

Model Terkoreksi _244.149a ₅ _48.830 _14.933 _.000

Intersep _160.449.795 _{1 160.449.795} _4,91E+07 _.000 Padat tebar _226.676 ₂ _113.338 _34.662 _.000 Ganti air _9.476 ₁ _9.476 _2.898 _.114 pdtbar*gantiair _7.997 ₂ _3.999 _1.223 _.329 Galat _39.238 ₁₂ _3.270 Total _160.733.183 ₁₈ Total Terkoreksi _283.387 ₁₇ a. R2= ,862 (Adjusted R Squared = ,804)

ANOVA pada penelitian budidaya ikan koridoras dengan perlakuan padat tebar dengan teknologi bioflok

LPH panjang

Sumber Jumlah Kuadrat db Kuadrat Tengah F p

Model Terkoreksi _.001a ₂ _.001 1.58 1 .281 Intersep _.853 ₁ _.853 2475 .129 .000 Perlakuan _.001 ₂ _.001 1.58 1 .281 Galat .002 6 .000 Total _.856 ₉ Total Terkoreksi _.003 ₈ a. R2 = .345 (Adjusted R Squared = .127)

LPH bobot

Sumber Jumlah Kuadrat db Kuadrat Tengah F p

Model Terkoreksi _.111a ₂ _.056 2.5 94 .15 4 Intersep 8.343 1 8.343 _.999388 .00₀ Perlakuan .111 2 .056 2.5₉₄ .15₄ Galat .129 6 .021 Total _8.583 ₉ Total Terkoreksi _.240 ₈ a. R2= .464 (Adjusted R Squared = .285) SR

Sumber Jumlah Kuadrat db Kuadrat Tengah F p

Model Terkoreksi _456.717a ₂ _228.358 41.6 16 .00 0 Intersep 32383.32 2 1 32383.3 22 590 1.544 .00 0 Perlakuan _456.717 ₂ _228.358 41.6 16 .00 0 Galat 32.924 6 5.487 Total 32872.96 3 9 Total Terkoreksi 489.640 8 a. R2= .933 (Adjusted R Squared = .910)

Lampiran 3 Nilai DNA, RNA dan Rasio RNA/DNA pada budidaya ikan koridoras dengan perlakuan padat tebar dan ganti air

No. Sampel Abs Protein (µg/µl) Purity (%) DNA (µg/µl) Pengenceran DNA x 80 (µg/µl) DNA ratio Bobot Hati (µg) [DNA]/µg Ratio RNA/dna

1 3000A1 0,538 0,1 94 26,9 80 2152 1,703 40000 0,054 2,0929 2 3000A2 1,534 0 118 76,7 80 6136 2,133 42000 0,146 0,2151 3 3000A3 1,019 0 108 50,9 80 4072 1,59 41000 0,099 0,5167 4 4500A1 0,51 0,1 94 25,5 80 2040 1,694 40000 0,051 1,9725 5 4500A2 1,495 0 98 74,75 80 5980 2,235 39000 0,153 0,7706 6 4500A3 2,481 0 129 124 80 9920 2,325 44000 0,225 0,1637 7 6000A1 2,202 0 127 110,1 80 8808 2,298 42000 0,210 0,3324 8 6000A2 1,689 0 118 84,4 80 6752 2,142 40000 0,169 0,3483 9 6000A3 2,033 0 123 101,6 80 8128 2,229 40000 0,203 0,4783 10 3000B1 2,019 0 126 101 80 8080 2,272 40000 0,202 0,2901 11 3000B2 0,66 0,1 101 33 80 2640 1,82 40000 0,066 1,3939 12 3000B3 2,401 0 108 120 80 9600 2,31 42000 0,229 0,5550 13 4500B1 1,809 0 145 90,4 80 7232 2,625 30000 0,241 0,5354 14 4500B2 2,62 0 125 131 80 10480 2,259 41000 0,256 0,6183 15 4500B3 1,344 0 117 67,2 80 5376 2,106 41000 0,131 0,4196 16 6000B1 1,154 0 109 57,7 80 4616 1,971 42000 0,110 0,3518 17 6000B2 1,577 0 119 78,8 80 6304 2,146 42000 0,150 0,9810 18 6000B3 1,511 0 117 75,6 80 6048 2,116 30000 0,202 0,4048

No. Sampel Abs Protein (µg/µl) Purity (%) RNA (µg/µl) Pengenceran RNA x 80 (µg/µl) RNA ratio Bobot Hati (µg) [RNA]/µg

1 3000A1 1,407 0,1 103 56,3 80 4504 1,855 40000 0,1126 2 3000A2 0,412 0 99 16,5 80 1320 1,794 42000 0,0314 3 3000A3 0,658 0,1 99 26,3 80 2104 1,786 41000 0,0513 4 4500A1 1,258 0,1 99 50,3 80 4024 1,796 40000 0,1006 5 4500A2 1,44 0,2 98 57,6 80 4608 1,77 39000 0,1182 6 4500A3 0,707 0,1 99 20,3 80 1624 1,791 44000 0,0369 7 6000A1 0,915 0,1 100 36,6 80 2928 1,801 42000 0,0697 8 6000A2 0,733 0 107 29,4 80 2352 1,93 40000 0,0588 9 6000A3 1,216 0,1 98 48,6 80 3888 1,765 40000 0,0972 10 3000B1 0,731 0,1 97 29,3 80 2344 1,761 40000 0,0586 11 3000B2 1,151 0,1 103 46 80 3680 1,864 40000 0,0920 12 3000B3 1,664 0,2 97 66,6 80 5328 1,756 42000 0,1269 13 4500B1 1,21 0,1 100 48,4 80 3872 1,81 30000 0,1291 14 4500B2 2,024 0,2 101 81 80 6480 1,822 41000 0,1580 15 4500B3 0,706 0,1 101 28,2 80 2256 1,825 41000 0,0550 16 6000B1 0,506 0 104 20,3 80 1624 1,887 42000 0,0387 17 6000B2 1,932 0,2 102 77,3 80 6184 1,841 42000 0,1472 18 6000B3 0,766 0,1 99 30,6 80 2448 1,782 30000 0,0816

Lampiran 4 Nilai DNA, RNA dan Rasio RNA/DNA pada budidaya ikan koridoras pada perlakuan padat tebar dengan teknologi bioflok

No Nama Sampel RNA Purity Ratio Protein Absorban RNA/mg Ratio

1 3000BFT1 75,1 104 1,880 0,1 1,877 1,502 3,0405 2 3000BFT2 71 105 1,897 0,1 1,776 1,420 2,3826 3 3000BFT3 64 118 2,100 0 1,620 1,280 3,2000 4 4500BFT1 70,5 106 1,920 0,1 1,763 1,410 4,3519 5 4500BFT2 73,4 106 1,923 0,1 1,835 1,468 1,6758 6 4500BFT3 85 106 1,913 0,1 2,125 1,700 2,1411 7 6000BFT1 94 102 1,839 0,2 2,351 1,880 1,1046 8 6000BFT2 56 102 1,934 0,1 1,408 1,120 2,4034 9 6000BFT3 75,3 104 1,824 0,1 1,883 1,506 2,2147

No Nama Sampel DNA Purity Ratio Protein Absorban DNA/mg

1 3000BFT1 24,7 99 1,800 0,1 0,495 0,494 2 3000BFT2 29,8 106 1,919 0 0,597 0,596 3 3000BFT3 20 97 1,754 0 0,400 0,400 4 4500BFT1 16,2 89 1,614 0,1 0,324 0,324 5 4500BFT2 43,8 104 1,873 0,1 0,876 0,876 6 4500BFT3 39,7 103 1,862 0,1 0,794 0,794 7 6000BFT1 85,1 123 2,215 0 1,703 1,702 8 6000BFT2 23,3 100 1,802 0 0,465 0,466 9 6000BFT3 34 101 1,831 0,1 0,680 0,680

Lampiran 5 Skema perhitungan jumlah molase yang diberikan pada perlakuan dengan teknologi bioflok ( De Schryver et al. 2008)

Bobot rata-rata ikan koridoras :A g

Biomassa : jumlah total ikan x A = B g

Kadar air cacing : 82,29 % (bk 17,71%) : Jumlah pakan: 100/17,71 x C = D g/hari

(cacing basah/hari)

Ekskresi : 75 % x F g = Gg N

Molase (C =45,27%) :100/45,27 x H g = I g

Pakan 5 % / hari dari biomassa : 5% x B = Cg/hari (berat kering cacing)

C/N (12) : 12 x G g = H gN Protein : 8,12 % x D = E g

Dalam dokumen Intensifikasi Budidaya Ikan Hias Koridoras (Corydoras Aeneus) (Halaman 82-98)