DAFTAR PUSTAKA - Species Identification of Meloidogyne on Carrot in Cianjur, West Java

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Lampiran 1 Susunan nukleotida M. hapla

Mh_Magelan : Mh_Cianjur : Mh_Hawai : Mh_Austral : Mh_Cina : Mh_Inggris : Mh_Amerika : Mh_Swiss : * 20 * 40 * 60 * 8 TGAAGGAAAAATCTTGGGAGAGTTACAGCGCTTCGCTATAATGAAATAAAATGCTAGTTATTTTGAAGCGAACTAAAAA TGAAGGAAAAATCTTGGGAGAGTTACAGCGCTTCGCTATAATGAAATAAAATGCTAGTTATTTTGAAGCGAACTAAAAA TGAAGGAAAAATCTTGGGAGAGTTACAGCGCTTCGCTATAATGAAATAAAATGCTAGTTATTTTGAAGCGAACTAAAAA TGAAGGAAAAATCTTGGGAGAGTTACAGCGCTTCGCTATAATGAAATAAAATGCTAGTTATTTTGAAGCGAACTAAAAA TGAAGGAAAAATCTTGGGAGAGTTACAGCGCTTCGCTATAATGAAATAAAATGCTAGTTATTTTGAAGCGAACTAAAAA TGAAGGAAAAATCTTGGGAGAGTTACAGCGCTTCGCTATAATGAAATAAAATGCTAGTTATTTTGAAGCGAACTAAAAA TGAAGGAAAAATCTTGGGAGAGTTACAGCGCTTCGCTATAATGAAATAAAATGCTAGTTATTTTGAAGCGAACTAAAAA TGAAGGAAAAATCTTGGGAGAGTTACAGCGCTTCGCTATAATGAAATAAAATGCTAGTTATTTTGAAGCGAACTAAAAA TGAAGGAAAAATCTTGGGAGAGTTACAGCGCTTCGCTATAATGAAATAAAATGCTAGTTATTTTGAAGCGAACTAAAAA : 79 : 79 : 79 : 79 : 79 : 79 : 79 : 79 Mh_Magelan : Mh_Cianjur : Mh_Hawai : Mh_Austral : Mh_Cina : Mh_Inggris : Mh_Amerika : Mh_Swiss : 0 * 100 * 120 * 140 * 1 TTGTGAGAAGAACAGGAGGATTTGAAAAGATTTTTCAAAAGTTCGAAAGATTACATGATTAATGTAAATTTTGGATTTC TTGTGAGAAGAACAGGAGGATTTGAAAAGATTTTTCAAAAGTTCGAAAGATTACATGATTAATGTAAATTTTGGATTTC TTGTGAGAAGAACAGGAGGATTTGAAAAGATTTTTCAAAAGTTCGAAAGATTACATGATTAATGTAAATTTTGGATTTC TTGTGAGAAGAACAGGAGGATTTGAAAAGATTTTTCAAAAGTTCGAAAGATTACATGATTAATGTAAATTTTGGATTTC TTGTGAGAAGAACAGGAGGATTTGAAAAGATTTTTCAAAAGTTCGAAAGATTACATGATTAATGTAAATTTTGGATTTC TTGTGAGAAGAACAGGAGGATTTGAAAAGATTTTTCAAAAGTTCGAAAGATTACATGATTAATGTAAATTTTGGATTTC TTGTGAGAAGAACAGGAGGATTTGAAAAGATTTTTCAAAAGTTCGAAAGATTACATGATTAATGTAAATTTTGGATTTC TTGTGAGAAGAACAAGAGGATTTGAAAAGATTTTTCAAAAGTTCGAAAGATTACATGATTAATGTAAATTTTGGATTTC TTGTGAGAAGAACAgGAGGATTTGAAAAGATTTTTCAAAAGTTCGAAAGATTACATGATTAATGTAAATTTTGGATTTC : 158 : 158 : 158 : 158 : 158 : 158 : 158 : 158 Mh_Magelan : Mh_Cianjur : Mh_Hawai : Mh_Austral : Mh_Cina : Mh_Inggris : Mh_Amerika : Mh_Swiss : 60 * 180 * 200 * 220 * CTCGCTGGTTAATCTTAATTCATTACAACTACTGGTAGCATGATGAATCATCAGGAATTTATGCCAGTACTCTGTTAGA CTCGCTGGTTAATCTTAATTCATTACAACTACTGGTAGCATGATGAATCATCAGGAATTTATGCCAGTACTCTGTTAGA CTCGCTGGTTARTCTTAATTCATTACAACTACTGGTAGCATGATGAATCATCAGGAATTTATGCCAGTACTCTGTTAGA CTCGCTGGTTAATCTTAATTCATTACAACTACTGGTAGCATGCTGAATCATCAGGAATTTATGCCAGTACTCTGTTAGA CTCGCTGGTTAATCTTAATTCATTACAACTACTGGTAGCATGATGAATCATCAGGAATTTATGCCAGTACTCTGTTAGA CTCGCTGGTTAATCTTAATTCATTACAACTACTGGTAGCATGATGAATCATCAGGAATTTATGCCAGTACTCTGTTAGA CTCGCTGGTTARTCTTAATTCATTACAACTACTGGTAGCATGATGAATCATCAGGAATTTATGCCAGTACTCTGTTAGA CTCGCTGGTTAATCTTAATTCATTACAACTACTGGTAGCATGATGAATCATCAGGAATTTATGCCAGTACTCTGTTAGA CTCGCTGGTTAaTCTTAATTCATTACAACTACTGGTAGCATGaTGAATCATCAGGAATTTATGCCAGTACTCTGTTAGA : 237 : 237 : 237 : 237 : 237 : 237 : 237 : 237 Mh_Magelan : Mh_Cianjur : Mh_Hawai : Mh_Austral : Mh_Cina : Mh_Inggris : Mh_Amerika : Mh_Swiss : 240 * 260 * 280 * 300 * AGTTGGTGAAGTGATTAATTTTCAATGAGATTAAAGCATAATTTGTCTTGTGCAAAGGAGATTATAATTTGCTGGCTTG AGTTGGTGAAGTGATTAATTTTCAATGAGATTAAAGCATAATTTGTCTTGTGCAAAGGAGATTATAATTTGCTGGCTTG AGTTGGTGAAGTGATTAATTTTCAATGAGATTAAAGCATAATTTGTCTTGTGCAAAGGAGATTATAATTTGCTGGCTTG AGTTGGTGAAGTGATTAATTTTCAATGAGATTAAAGCATAATTTGTCTTGTGCAAAGGAGATTATAATTTGCTGGCTTG AGTTGGTGAAGTGATTAATTTTCAATGAGATTAAAGCATAATTTGTCTTGTGCAAAGGAGATTATAATTTGCTGGCTTG AGTTGGTGAAGTGATTAATTTTCAATGAGATTAAAGCATAATTTGTCTTGTGCAAAGGAGATTATAATTTGCTGGCTTG AGTTGGTGAAGTGATTAATTTTCAATGAGATTAAAGCATAATTTGTCTTGTGCAAAGGAGATTATAATTTGCTGGCTTG AGTTGGTGAAGTGATTAATTTTCAATGAGATTAAAGCATAATTTGTCTTGTGCAAAGGAGATTATAATTTGCTGGCTTG AGTTGGTGAAGTGATTAATTTTCAATGAGATTAAAGCATAATTTGTCTTGTGCAAAGGAGATTATAATTTGCTGGCTTG : 316 : 316 : 316 : 316 : 316 : 316 : 316 : 316 Mh_Magelan : Mh_Cianjur : Mh_Hawai : Mh_Austral : Mh_Cina : Mh_Inggris : Mh_Amerika : Mh_Swiss : 320 * 340 * 360 TCATTTTAATCTTTAATCATATTTTCGCAAAGGGATTAAAATGGTGGATT TCATTTTAATCTTTAATCATATTTTCGCAAAGGGATTAAAATGGTGGATT TCATTTTAATCTTTAATCATATTTTCGCAAAGGGATTAAAATGGTGGATT TCATTTTAATCTTTAATCATATTTTCGCAAAGGGATTAAAATGGTGGATT TCATTTTAATCTTTAATCATATTTTCGCAAAGGGATTAAAATGGTGGATT TCATTTTAATCTTTAATCATATTTTCGCAAAGGGATTAAAATGGTGGATT TCATTTTAATCTTTAATCATATTTTCGCAAAGGGATTAAAATGGTGGATT TCATTTTAATCTTTAATCATATTTTCGTAAAGGGATTAAAATGGTGGATT TCATTTTAATCTTTAATCATATTTTCGcAAAGGGATTAAAATGGTGGATT : 366 : 366 : 366 : 366 : 366 : 366 : 366 : 366

Lampiran 2 Susunan nukleotida M. javanica

Mj_Cianjur : Mj_Iran : Mj_Spain : Mj_Spain2 : Mj_Brazil : Mj_Brazil2 : Mj_USA : Mj_USA2 : Mj_China : Mj_China2 : Mj : * 20 * 40 * 60 * 80 * 100 *

CCTAAAAAAAAAAAAAAAAATAAACACGCGAAAAACCTTTAAAAAACCGCCGGTTTTATTAGCCCCCCCTTTTTAAAAGGCCCCCCTATAAAAGACCCCCCTTTCAAAAAACCCC

CTTTATGTGATGTTCAAATTTGAATTCGCAATGAAATGATCGTTGTGAAACGGCTGTCGCTGGTGTCTAAGTGTTGCTGATACGGTTGTGAACGTCCGTGGCTGTATATGTGGTG

CTTTTTGTGATGTTCAAATTCGAATAGTCTCAACGTTTATCGTTGTGAAACGGCTGTCGCTAGTGTCTAGGTGTTGCTGATTCAGCTGTCAACGTCCGTGGCTGAATATGAGGTG

CTTTATGTGATGTTCAAATTTGAATTCGCAATGAAATGATCGTTGTGAAACGGCTGCCGCTGGTGTCTAAGTGTTGCTGATACGGTTGTGAACGTCCGTGGCTGTATATGTGGTG

CCTACTAAAAAATACAAAATTAAATACGCGTAAAAGCTCTAATTAAAGCCCGGTTCTATTAGATGCCCATGTCTAATAGATGCCCTTCTAATAGAAGCCCATCTCTAATATAAGC

CTTTATGTGATGTTCAAATTTGAATTCGCAATGAAATGATCGTTGTGAAACGGCTGTCGCTGGTGTCTAAGTGTTGCTGATACGGTTGTGAACGTCCGTGGCTGTATATGTGGTG C T t A t cAAA tt AAt cgC aa T tt CGG T t g tg C a gT T Gat C tT T Aa G cc t a At t

: 115 : 115 : 115 : 115 : 115 : 115 : 115 : 115 : 115 : 115 : 115 Mj_Cianjur : Mj_Iran : Mj_Spain : Mj_Spain2 : Mj_Brazil : Mj_Brazil2 : Mj_USA : Mj_USA2 : Mj_China : Mj_China2 : Mj : 120 * 140 * 160 * 180 * 200 * 220 * CCCCTCAAGGCGGCCCAAAAAAATAACACCCCCCCCCCCCCCCCCATGAGCAAGTTGATACGAAACAGTGTCTTTTTTTTTAGCTAAGGCCACCGTAACAGAAACATAATCAATA ACATGTTAGGACTCTAATGAGTTTAAGACCTAATGAGCCTCTTAAGTGAGGCCGCCAGCAACCTTTTTTTTCTCTACATTTTAAAAAAAAAACTAAAATTCTATCCTTATCGGTG

ACATGTTAGGACTCTAATGAGTTTAAGACCTAATGAGCCTCTTAAGTGAGGCCGCCAGCAACCTTTTTTTTCTCTACATTTTAAAAAAAAAACTAAAATTCTATCCTTATCGGTG

ACATGTTAGGACCTTAATGGGTTTAAGACTTAATGAGCCTCTTAAGTGAGGACGCCAGCAATATTTTTTTCAACTATTTTTTTTAAAAAACGATAAATTTTTATCCTTATCGGTG

ACATGTTAGGACTCTAATGAGTTTAAGACCTAATGAGCCTCTTAAGTGAGGCCGCCAGCAACCTTTTTTTTCTCTACATTTTAAAAAAAAAACTAAAATTCTATCCTTATCGGTG

CCACTCAAGGAGGCCAATGAAAATATAAACGCCTAGGACTTTAATTAGAGCATTTTCGATAAATTTATTTATTAGGTTTTCTGTTACGGTGGCGTTAGCTATATAAAAGACACTG

ACATGTTAGGACTCTAATGAGTTTAAGACCTAATGAGCCTCTTAAGTGAGGCCGCCAGCAACCTTTTTTTTCTCTACATTTTAAAAAAAAAACTAAAATTCTATCCTTATCGGTG

Ca AGGa c aAtga TAa Acc t gcCtct a tGAG g g aa ttt tTt t t TTtt Aa c A t tAtc t atC Tg : 230 : 230 : 230 : 230 : 230 : 230 : 230 : 230 : 230 : 230 : 230 Mj_Cianjur : Mj_Iran : Mj_Spain : Mj_Spain2 : Mj_Brazil : Mj_Brazil2 : Mj_USA : Mj_USA2 : Mj_China : Mj_China2 : Mj : 240 ATTTATCGAAAATGCTGAT GATCACTAGGCTCGTGGAT GATCACTAGGCTCGTGGAT GATCACTAGGCTCGTGGAT GATCACTCGGCTCGTACGT GATCACTCGGCTCGTACGT GATCACTCGGCTCGTGGAT GATCACTAGGCTCGTGGAT TCGTATCAAACTTGTAGAT TCGTATCAAACTTGTAGAT GATCACTAGGCTCGTGGAT t A ct Gt gaT : 249 : 249 : 249 : 249 : 249 : 249 : 249 : 249 : 249 : 249 : 249

Lampiran 3 Pembuatan bufer ekstrak SDS (untuk nematoda puru akar betina)

Bahan Konsentrasi akhir buffer Volume (ml)

1M Tris HCl pH 8.5 200 mM 1.0

5M NaCl 250 mM 2.5

0.5 M EDTA pH 8.0 25 mM 2.5

Dalam dokumen Species Identification of Meloidogyne on Carrot in Cianjur, West Java (Halaman 42-50)